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Comprehensive collection of genes and comparative analysis of full-length transcriptome sequences from Japanese larch (Larix kaempferi) and Kuril larch (Larix gmelinii var. japonica)
BMC Plant Biology volume 22, Article number: 470 (2022)
Japanese larch (Larix kaempferi) is an economically important deciduous conifer species that grows in cool-temperate forests and is endemic to Japan. Kuril larch (L. gmelinii var. japonica) is a variety of Dahurian larch that is naturally distributed in the Kuril Islands and Sakhalin. The hybrid larch (L. gmelinii var. japonica × L. kaempferi) exhibits heterosis, which manifests as rapid juvenile growth and high resistance to vole grazing. Since these superior characteristics have been valued by forestry managers, the hybrid larch is one of the most important plantation species in Hokkaido. To accelerate molecular breeding in these species, we collected and compared full-length cDNA isoforms (Iso-Seq) and RNA-Seq short-read, and merged them to construct candidate gene as reference for both Larix species. To validate the results, candidate protein-coding genes (ORFs) related to some flowering signal-related genes were screened from the reference sequences, and the phylogenetic relationship with closely related species was elucidated.
Using the isoform sequencing of PacBio RS ll and the de novo assembly of RNA-Seq short-read sequences, we identified 50,690 and 38,684 ORFs in Japanese larch and Kuril larch, respectively. BUSCO completeness values were 90.5% and 92.1% in the Japanese and Kuril larches, respectively. After comparing the collected ORFs from the two larch species, a total of 19,813 clusters, comprising 22,571 Japanese larch ORFs and 22,667 Kuril larch ORFs, were contained in the intersection of the Venn diagram. In addition, we screened several ORFs related to flowering signals (SUPPRESSER OF OVEREXPRESSION OF CO1: SOC1, LEAFY: LFY, FLOWERING Locus T: FT, CONSTANCE: CO) from both reference sequences, and very similar found in other species.
The collected ORFs will be useful as reference sequences for molecular breeding of Japanese and Kuril larches, and also for clarifying the evolution of the conifer genome and investigating functional genomics.
Coniferous tree species are often dominant in boreal forests where they play an important role in the ecology of forest ecosystems. The genetic and evolutionary characteristics of conifers have been widely studied and numerous comparative studies with other seed-producing plants have been conducted . Conifers are frequently selected for breeding, as they provide important building materials and fuel resources for humans. Further, afforestation also increases carbon storage in forests [2, 3], and is therefore regarded as an effective mitigation measure against climate change .
The coniferous genus Larix contains ten species and seven varieties with wind-mediated pollen and seed dispersal that are often dominant in the cool-temperate and subarctic forests of the northern hemisphere . Japanese larch (Larix kaempferi) is among the most important forestry tree species in northern Japan. More than 500 Japanese larch plus trees were selected from both natural and man-made larch forests, which together cover an area of 1 million ha and account for approximately 10% of the artificial forests in Japan . Japanese larch has suitable characteristic for forestry and was introduced to Hokkaido from the central mountainous region of Honshu since the early part of the last century . However, shortly after Japanese larch was introduced to Hokkaido, the species was adversely affected by vole grazing and dieback disease [7,8,9]. On the other hand, Kuril larch (Larix gmelinii var. japonica), which was introduced to Hokkaido from the Kuril Islands and Sakhalin, showed higher resistance to vole grazing than Japanese larch, but the growth was slower than that of Japanese larch. To overcome the disadvantages of the two species, hybrid seedlings of Kuril larch and Japanese larch were produced by artificial interspecific crossing. The hybrid larch, which exhibits heterosis in the form of rapid juvenile growth and high resistance to vole grazing [7,8,9], is currently one of the most important species in Hokkaido.
Recent advances in sequencing technology and bioinformatics have enabled researchers to perform genome-wide surveys of various economically and/or ecologically important crops (e.g., rice , tomato , soybean , and maize ). However, the amount of reference genome information that is available for coniferous species is currently limited to several species (e.g., Picea abies , Pinus taeda , Pinus lambertiana , Pseudotsuga menziesii var. menziesii , and Pinus tabuliformis , Larix sibilica , Sequoia sempervirens , Larix kaempferi ), mainly because their large genomes and complex genomic structures have hindered the precise elucidation of their genomes. Although a reference genome sequence for the Japanese larch has been published  and deposited in GenBank/DDBJ/EMBL (Accession No.: WOXR00000000), genetic information is not available. Compared to crop species, reference genome information for coniferous species has not been used effectively. Although numerous examples of polymorphisms and transcriptome information have been collected for coniferous species based on expressed sequence tag (EST) data, correlations between various traits for molecular breeding and the geographical structure of species have been performed mainly without reference genome sequences (e.g., Cryptomeria japonica [22, 23], Picea glauca [24, 25], P. abies [26, 27], Pinus thunbergii , P. taeda [29,30,31]).
RNA sequencing (RNA-Seq) has been widely used to construct transcript sequences, such as unigenes, for numerous of plant species, especially, for plant species with large genome size, such as forest trees. Consequently, a large amount of transcriptome data for coniferous species that do not require a reference genome has been collected for various research applications [1, 32,33,34,35,36]. RNA-Seq facilitates the accurate and large-scale sequencing of cDNA and is effective for characterizing genetic models without reference genomes . However, RNA-Seq short-reads are often not full-length transcripts, which means that they require large-scale computational assemblies to reconstruct transcript sequences; there is thus a trade-off between sampling depth and data integrity [38, 39]. Further, constructing full-length transcript sequences using short-reads is often complicated by factors such as mis-assemblies and low coverage of reads. Since short-reads can cause mis-assembly of transcripts, long-read sequencing techniques are considered preferable for reconstructing full-length transcripts. The single-molecule real-time (SMRT) sequencing technology developed by Pacific Biosciences (PacBio Inc.) has facilitated the elucidation of highly accurate long-reads, which can overcome the problems introduced by the short-read approach. In addition, the SMRT sequencing technology has the advantage of producing full-length cDNA sequences and can be used to characterize the structural variation of isoforms derived from alternative splicing [35, 40]. However, due to low coverage, obtaining all of the transcripts using only long-read data would be difficult. Therefore, to efficiently construct exhaustive cDNA sequences, it is desirable to perform a combination of short- and long-read sequencing. To do this, short-read sequences are assembled de novo before being merged with long-read sequences using a clustering approaches.
The objective of this study was to establish a comprehensive collection of full-length transcriptome sequences for Japanese larch and Kuril larch which can be applied to molecular breeding of both species and hybrids. To this end, we identified the Japanese larch and Kuril larch reference transcriptome sequences using a combination of long-read (isoform sequence with PacBio RS ll isoform (Iso-Seq)) and short-read (RNA sequence with Illumina (RNA-Seq)) approaches, and the obtained reference transcriptome sequences of both species, which were then compared to clarify their similarities. Moreover, in order to verify the usefulness of the constructed full-length transcriptome sequences, ORFs related to flowering-related signal genes were screened using reference sequences. Then, the sequence similarities of these candidate ORFs and the phylogenetic relationships with closely related species were compared. Larix species, they show considerable year-to-year variation in flower production and few consecutive flowering events are typically observed . In addition, mast seedling makes it difficult for forestry managers to formulate efficient tree breeding programs for these species. As a result, we need to elucidate the genetic processes responsible for controlling flowering need to be elucidated using molecular methods, but information on the flowering genes of these species is scarce. The reference sequences constructed in this study shows that these sequences contained the background information that could be used to elucidate the flowering mechanisms of the genus Larix.
The collected genetic information will also be used as a transcriptome reference for future research on the genus Larix.
ORF prediction for the transcriptomes obtained by isoform sequencing (Iso-Seq)
Bulked RNA extracted from three tissue types (cambium, needles, and shoots) was sequenced to achieve wide coverage of the transcriptome using PacBio isoform sequencing for each of the two Larix species. A total of 501,286 and 459,268 reads of inserts (ROIs) were generated, with 1,566,511,985 and 1,224,443,307 nucleotides obtained from nine (insert size: 1–2 kb, 2–3 kb, 3–6 kb, 5–10 kb) and ten (1–2 kb, 2–3 kb, 3–4 kb, 4–10 kb) SMRT cells from Japanese and Kuril larches, respectively (Table 1). For Japanese and Kuril larches, the mean read lengths were 3,125 and 2,666 bp, respectively, with 246,070 (49.1%) and 248,940 (54.2%) full-length (FL) ROIs and 242,646 (48.4%) and 246,043 (53.6%) FL non-chimeric ROIs, respectively. After using the Iso-Seq clustering algorithm, (iterative clustering for error correction (ICE)), a total of 91,714 and 83,026 high-quality, polished isoforms and 40,693 and 50,225 low-quality polished isoforms of FL non-chimeric ROIs were obtained by Quiver polishing, respectively. Finally, high-quality, non-redundant isoforms were obtained comprising 79,832 and 66,002 sequences, with lengths ranging from 300–8,880 bp (mean 2,715 bp) and 307–10,117 bp (mean 2,446 bp), respectively. BUSCO analysis revealed that 65.1% and 71.9% of the 1,375 BUSCOs in embryophytes (odb10) were found with completeness in Japanese larch and Kuril larches, respectively (72.9% and 78.6% when fragmented BUSCOs were included).
ORF prediction was conducted to obtain high-quality non-redundant isoforms using ANGEL software. For the Japanese and Kuril larches, 80,557 and 67,332 ORFs were predicted, ranging from 145–7,956 bp (mean 1,356 bp) and 146–6,609 bp (mean 1,172 bp), respectively (Table 2). Among the total predicted ORFs, 37,508 (46.6%) and 29,372 (43.6%), were confident ORFs (confident-complete, 5’ partial, 3’ partial, internal), respectively. In the BUSCO analysis, the completeness of the confident ORFs in Japanese and Kuril larches was 46.8% and 52.3%, respectively (Table 3).
ORF prediction of the transcriptomes obtained by de novo assembly of short-read sequences (RNA-Seq)
The RNA-Seq short-reads sequenced from the two of Larix species were assembled to compare the differences between the transcript sequences and their expression. A total of approximately 1 billion raw reads for the two species were generated and the Q20 and Q30 quality score cutoffs and GC contents (%) are shown in Table 4. The in silico normalized reads for the Japanese and Kuril larches (182,578,084 reads and 209,039,560 reads, respectively) were applied to the first de novo assembly by using Trinity v2.8.5, and 912,369 and 1,133,931 contigs with N50 length of 1,112 bp and 1,016 bp were obtained for the Japanese and Kuril larches, respectively (Tables 4, 5). The trimmed reads were mapped against the contigs, and fragments per kilobase per million fragments mapped (FPKM) values > 1 were selected as “unitranscripts” to remove low-quality transcripts including mis-assemblies. Next, the longest unitranscripts from each gene locus were selected as unigenes. Finally, 58,396 and 36,972 unigene sequences with lengths ranging from 188–17,703 bp (mean 1,171 bp) and 185–18,429 bp (mean 1,334 bp), and N50 lengths of 2,246 bp and 2,474 bp were obtained for Japanese and Kuril larches, respectively (Table 5).
ORF predictions based on the unigenes were conducted using the TransDecoder program for the two larches. A total 27,130 and 20,207 ORFs with lengths ranged from 255–14,493 bp (mean 996 bp) and 255–14,493 bp (mean 1,111 bp) and N50 lengths of 1,341 bp and 1,500 bp were predicted for the Japanese and Kuril larches, respectively (Table 6). In the BUSCO analysis, the completeness of the predicted ORFs was 82.0 and 87.5 complete (%) in the Japanese and Kuril larches, respectively.
Integration of ORFs obtained by Iso-Seq and RNA-Seq analyses
To examine the entire full-length transcriptome sequences, the ORF sequences (high-quality full-length isoforms) obtained by Iso-Seq analysis were merged with ORFs obtained by RNA-Seq analysis. As a result, 107,687 and 87,539 ORFs were generated for the Japanese and Kuril larches, respectively (Table 7). Finally, the merged ORFs were subjected to cluster analysis using cutoffs of 90% identity and 90% length coverage with CD-HIT-EST. The longest ORF was then selected from each cluster as a representative transcript. As a result, 50,690 and 38,684 ORFs were obtained for Japanese and Kuril larches, respectively. The number of ORFs derived from Iso-Seq and RNA-Seq in Japanese larch were 32,610 (64.3%) and 18,080 (35.7%), respectively, and those in Kuril larch were 27,490 (71.1%) and 11,194 (28.9%), respectively. The total lengths of the merged ORFs were 53.8 and 39.0 Mb, and N50 lengths, GC content and BUSCO completeness values were 1,473 bp and 1,413 bp, 46.4% and 45.1%, and 90.5% and 92.1% in the Japanese and Kuril larches, respectively (Table 7). The obtained Japanese larch ORFs were then mapped to the genome sequence of Japanese larch isolate RF27, which was obtained from the NCBI database (Accession No.: WOXR00000000). As a result, 40,607 ORFs (80.1%) were mapped to 52,341 loci, and of these ORFs, 32,877 were uniquely mapped to specific loci. The 52,054 ORFs were extracted from the mapped regions, and a BUSCO analysis generated completeness values of 73.0% and fragmented values of 13.0%. Based on these findings, it is considered that 86.0% of the ORFs constructed in this study correspond to protein-encoding regions in the Japanese larch genome.
Comparison of ORFs between Japanese and Kuril larches
To clarify the interspecific relationship between the Japanese and Kuril larches, we compared 50,690 and 38,684 ORFs from the two species using OrthoFinder (Fig. 1), and searched for sequences in the NCBI’s non-redundant protein (NR) database using DIAMOND software with the more sensitive mode. The results are shown in the Venn diagram in Fig. 1 and are summarized in Table 8. A total of 19,813 clusters, comprising of 22,571 Japanese larch ORFs and 22,667 Kuril larch ORFs, were contained in the intersection of the Venn diagram. The BUSCO completeness values for the ORFs from the Japanese and Kuril larches were 88.5% and 90.1%, respectively. Among these ORFs, the number of ORFs with significant (E-value ≤ 1e-10) matches against plant entries in GenBank nucleotide divisions (gbpln) in the NR database was 20,335 (90.1% of all ORFs) and 20,464 (90.3% of all ORFs) for Japanese and Kuril larches, respectively. A total of 28,119 ORFs (including seven clusters, which consisted of 33ORFs) were unique to Japanese larch, with a BUSCO completeness of 16.5% and 18,919 ORFs matches against gbpln. For the Kuril larch, a total of 16,017 ORFs (including five clusters, which consisted of 15 ORFs) were species-specific, with a BUSCO completeness of 9.9% and 11,534 ORFs matches against gbpln. In the intersection of the Venn diagram, approximately 90% of ORFs from the two species were annotated in gbpln (Additional File 1). However, in the species-specific region of the Venn diagram, the proportion of “no hits” against the NR database was relatively high, and the proportion of gbpln “hits” was relatively low (Additional File 1). To assign functional characteristics to the ORFs from the two species, a gene ontology (GO) analysis was performed. After classifying the ORFs into the GO categories (BP: biological process, CC: cellular component, MF: molecular function), the distribution of the GO terms was found to be similar between the two species (Additional File 2).
Comparison of ORFs against other species
The ORFs were searched against the NR database using the DIAMOND program with more sensitive mode (Additional File 3, 4). For the Japanese and Kuril larches, a total of 39,661 ORFs (78.2% of all ORFs) and 31,336 ORFs (81.0% of all ORFs) had significant BLAST matches (E-value ≤ 1e-10), respectively (Additional File 3, 4, 5). Similarities between the 50,690 and 38,684 ORFs from Japanese and Kuril larches were searched by BLASTP with an E-value cutoff of 1e-10, respectively. The finding showed that 41,301 ORFs (81.5% of all ORFs) from Japanese larch had significant BLAST matches with the ORFs from Kuril larch (Additional File 3, 4, 5). Conversely, 34,933 ORFs (90.3% of all ORFs) from Kuril larch were matched to ORFs from Japanese larch (Additional File 3, 4, 5). When we compared the protein sequence similarities of the genes among A. sachalinensis, P. lambertiana, Populus trichocarpa, Arabidopsis thaliana, and C. japonica using a BLASTP searches with an E-value cutoff of 1e-10 (Additional File 3, 4, 5), the number ORFs shared by each of these species with Japanese and Kuril larches was 38,513 and 32,794 for Abies; 36,156 and 30,821 for Pinus; 36,149 and 30,739 for Cryptomeria; 32,820 and 27,978 for Populus; 32,351 and 27,572 for Arabidopsis, respectively.
Phylogenetic analysis of flowering-related genes
Twenty-five candidate ORFs that were similar to the Type II MADS-box gene were founded in Japanese (9 ORFs) and Kuril larches (16 ORFs). A phylogenetic tree was constructed together with previously identified MADS-box genes sequence from Larix, Pinus, Picea, Cryptomeria, Gnetum, Arabidopsis, Coleochaete, and Chara (Fig. 2, Additional File 6). Among the 25 Larix ORFs identified in this study, 16 were shared by the members of a subgroup in the Soc1 clade (TM3 clade) including Pinus and Picea sequences. Among the 16 Larix ORFs, five sequences (LG_I_c04727_02447, LG_I_c22080_12952, LG_I_c04996_02610, LG_I_c19639_11427, and LG_T_003842_c00_g01_i09.p1) were similar to the previously reported sequences in the Japanese larch, but the other 11 sequences were located in different clades and would thus be novel candidate TM3-like genes. The four ORFs were annotated as the LEAFY/NEEDLY gene by the BLASTP searches. The amino acid sequences were identical in the two larch species, and similar to those previously reported for Japanese and Kuril larches. In the phylogenetic tree, the detected LEAFY/NEEDLY genes were similar to the orthologous sequences in Pinus and Picea species, which belonged to the same clade (Fig. 3, Additional File 7). Based on the BLASTP searches, three ORFs that were similar to FLOWERING Locus T-like genes/Mother of FLOWERING Locus T-like (MFT-like) genes were annotated in both of the larches. In the phylogenic tree, four out of six of the Larix ORFs were assigned to the clades FTL1 and FTL2. Each of the Larix FTL1 and FTL2 sequences were accompanied by orthologous sequences of Pinus and Picea species within the clade (Fig. 4, Additional File 8). As for MFT-like genes, two amino acid sequences (LK_I_c16100_79332 and LG_T_009821_c00_g01_i01.p1) were identical between the two larch species and the sequences were grouped in the same cluster as Pinus_MFT, Picea_MFT1 in the phylogenetic tree (Fig. 4, Additional File 8). Four ORFs of CONSTANCE (CO)-like genes were annotated from the BLASTP searches. Two of the four ORFs annotated as CO genes were located in the coniferous tree COL1 gene clade. The other two ORFs were located in the COL2 gene clade with the Picea_COL2 in the phylogenetic tree (Fig. 5, Additional File 9). These results showed that the Japanese larch ORFs were similar to the previously reported sequences in the Japanese larch, and that the Kuril larch ORFs contained novel candidate genes.
Full-length (FL) transcriptome collection
In this study, we successfully sequenced 107,687 and 87,539 transcripts (Iso-Seq + RNA-seq) derived from total-RNA collected from the shoots, cambium, and needles of Japanese and Kuril larches, respectively (Table 7). To remove any redundancy in the unigenes sequences derived from alternative splicing or heterozygosity in the Japanese and Kuril larches, the ORFs were clustered using a minimum identity of 90% and a high coverage of at least 90% using CD-HIT-EST. Based on these sequences, a total of 50,690 and 38,684 ORFs were predicted for Japanese and Kuril larches, respectively. The number of the ORFs obtained by Iso-Seq was approximately three-fold that obtained by RNA-Seq; however, the gene coverages estimated by BUSCO for Iso-Seq data was lower than that estimated for RNA-Seq data (Table 2,6). These results could be attributed to the following factors. First, since the Iso-Seq data are derived from long-reads, they reflect full-length sequences, which enables more accurate ORF estimation, and can also provide isoform information; however Iso-Seq are less comprehensive than a short-read data. Second, while short-read data have a relatively higher gene coverage than Iso-Seq data, primarily due to the large amount of sequence data obtained, instances of miss-assembly during the assembly process can reduce the accuracy of ORF prediction from unigenes . Indeed, the likelihood of mis-assembly is particularly high in genomes of coniferous trees because the genomes are large, complex and have numerous repetitive sequences [42, 43]. Therefore, we attempted to construct the ORFs using the Iso-Seq and RNA-Seq reads by a clustering approach. As a result, we constructed unigenes including approximately 90% of the single core genes defined by BUSCO analyses in both the Japanese and Kuril larches.
Transcript sequencing has been reported for various coniferous species, and the number of unigenes has varied among species. For example, C. japonica has 34,731 unigenes ; P. bungeana has 88,092 unigenes ; A. sachalinensis has 158,542 transcripts ; P. taeda has 50,172 gene models (15,653 high confidence) ; P. abies has 28,345 high-confidence genes ; P. menziesii var. menziesii has 22,257 high-quality full-gene models ; P. lambertiana has 85,053 gene models (13,936 high confidence) ; P. tabuliformis has 80,495 genes ; S. sempervirens has 118,906,495 genes  and Larix kaempferi has 299,637 assembled transcripts , 85,446 unigenes . In this study, the number of ORFs predicted using Iso-Seq and RNA-Seq data was comparable to values reported in these previous studies, especially when the sequences obtained in this study are compared with previously published Japanese larch data; for example, the assembly statistics were similar except that the number of ORFs was approximately 1/6 this study compare to previous study . The BUSCO completeness values estimated in this study were higher than those for the P. lambertiana and P. taeda genomes . These findings indicate that the approach used to construct the unigenes by merging Iso-Seq and RNA-Seq sequences was very effective for developing a comprehensive and extensive collection of full-length transcriptome sequences.
Comparing the ORFs collected from the two larch species, 19,813 clusters comprising 22,517 and 22,667 ORFs for Japanese and Kuril larches were located in the intersection of the Venn diagram shown in Fig. 1. Only seven clusters were unique to Japanese larch and five to Kuril larch; these clusters contained 33 and 15 ORFs, respectively, and 28,086 and 16,002 ORFs were not clustered, respectively. The BUSCO completeness for the common ORFs among Japanese and Kuril larches was 88.5% and 90.1%, respectively, whereas that for the species-specific ORFs was 16.5% and 9.9%, respectively (Fig. 1). These findings suggest that highly comprehensive and reliable ORFs were found in the intersection of the Venn diagram. Conversely, in the specific regions of the Venn diagram (i.e., the species-specific regions to the sides of the intersection) may contain species-specific sequences, and the possibility of contamination cannot be unfortunately, completely ruled out. In addition, the proportion of ORFs in this region that could not be annotated is relatively high, and while these ORFs may be novel sequences or long non-coding RNAs, they are among the factors that decrease the BUSCO score (Fig. 1). The ORFs that we obtained and characterized provide a valuable resource for a molecular breeding and comparative study of functional genetics in coniferous species.
Phylogenetic analysis of flowering signal genes
In A. thaliana, flowering control signals are induced by multiple external environmental and internal physiological factors, such as long days, autonomous vernalization, age, and gibberellins, and multiple control pathways are involved in transmitting changes in these factors . Moreover, several key transcription factor genes, such as SOC1, LFY, FT and CO, that integrate multiple control pathways based on these environmental factors have been identified . In Larix species, there is considerable year-to-year variation in flower production and, few consecutive flowering events are typically observed ; indeed, this is one of the problems that needs to be addressed in breeding. In this study, the key transcription factor genes, SOC1, FT, and LFY, and CO candidate ORFs involved in the regulation of flowering onset in Japanese and Kuril larches were screened from the collected ORFs using A. thaliana annotation information, and a phylogenetic tree was generated using similar genes found in other coniferous tree species.
When SOC1 is induced at the top of the shoot, SOC1, along with AGL24, directly activate the flower meristem identity gene LFY in A. thaliana . SOC1-like genes have been isolated from several gymnosperms (e.g., G. gnemon , P. abies [48, 49], P. radiata , and L. kaempferi ). Further, gene expression and transgenic studies of SOC1-like genes isolated from Japanese cedar revealed that these genes play important roles in development of male and female strobili . However, in Japanese larch and P. tabuliformis, the expression of some SOC1 homologs was reported to increase with age, implying that SOC1 plays a different role in Arabidopsis [51, 53, 54]. In the phylogenetic tree generated in this study, many Japanese and Kuril larch ORFs were located in polyphyletic clades of the TM3-related gene group to which SOC1 of Arabidopsis and AGL24-like ORFs from the two larch species were grouped together with the outgroup of AGL24 in A. thaliana (Fig. 2); however, both ORFs are reflected the phylogenetic relationships among these species (Fig. 2). Additionally, in this study, the SOC1 homologs of Kuril larch were more similar to the Japanese larch SOC1 homolog previously reported than the one obtained from Japanese larch in this study. Presently, reasonable explanations were unfortunately unable to be found. This could be clarified by some verifications (for example, comparison of gene expression patterns of each homolog) in the future.
In angiosperms, LEAFY/FLORICAULA (LFY/FLO) is regulated directly by SOC1, which regulates downstream MADS-box B-Class and C-class genes involved in floral meristem determinacy [46, 55, 56]. All major groups of existing gymnosperms are known to carry two paralogous LFY-like genes (LFY/NEEDLY) [57,58,59] which contribute directly to reproductive structure formation . In P. abies, it has been clarified that NEEDLY is a potential mediator in the transition from vegetative shoots to female cones . In P. taeda, the NEEDLY gene encodes a functional ortholog of the FLORICAULA/LEAFY genes of angiosperms . In this study, the ORFs were screened in the two larch species, and the sequences were almost identical (Fig. 3, Additional file 7). The LFY-like genes in the genus Larix reflected the phylogenetic relationships of both LFY-like and NEEDLY genes, which is consistent with previously reported results .
FLOWERING LOCUS T (FT) which belongs to the CENTRORADIALIS/TERMINAL FLOWER 1/SELF-PRUNING (CETS) gene family, is an important floral integrator that is induced by long-distance signals that contributes to the activation of the meristem identity gene class A (AP1:APETALA1) in Arabidopsis [63,64,65]. In P. abies, genes of the CET gene family were isolated (MOTHER OF FT AND TFL1 (MFT)-like clade, PaMFT1 and PaMFT2, and FT and TERMINAL FLOWER 1(TFL1) clades, PaFTL1 and PaFTL2); when overexpressed in A. thaliana, the PaFTL1 and PaFTL2 genes suppress flowering, but PaMFT1 and PaMFT2 have no effect . In particular, PaFTL2 has been shown to control growth cessation and bud set in response to short day (SD) length, and bud burst in response to elevated temperatures [67, 68]. A Pinus sylvestris homolog to PsFTL2 employs a similar mechanism for regulating the timing of growth cessation in conifers . In the phylogenetic tree estimated in the present study, the ORFs related to FTL1, FTL2 and MFT were screened in the two larch species. The amino acid sequences for each ORF were the same and were located in the Picea and Pinus subgroup on the phylogenetic tree (Fig. 4, Additional file 8). Therefore, the FTL2 genes in genus Larix may have functions that are similar to those reported previously [67,68,69].
CONSTANS (CO) is involved in the photoperiod flowering pathway . Specifically, in Arabidopsis, CO senses the photoperiod and integrates the circadian clock and light signals to induce downstream photoperiod-specific FT transcription . In gymnosperms, a CO-like gene has been isolated and identified in several species (P. sylvestris, AFV78277.1, P. radiata, AAD22518.1, P abies, [68, 70], L. kaempferi: AHA4436). In P. abies, two CO-like genes, PaCOL1 and PaCOL2, were isolated, and transcription levels of these genes in shoots and needles were significantly reduced under SD prior to growth cessation and bud formation, suggesting their involvement in the photoperiodic control of shoot elongation . In the phylogenetic tree generated in the present study, the ORFs that were similar to the CO-like gene in the genus Larix accurately reflected the phylogenetic relationships of these species and formed a subgroup with the genus Picea (Fig. 5). In both of the larches, the obtained COL2 ORFs were similar to a previously registered sequence (AHA44836) (Fig. 5, Additional file 9). Since larches are deciduous, the detection of SD prior to growth cessation is important, and a clear SD response mechanism may exist in larch species.
However, further research is needed to identify and clarify the role of screened flowering signal-related ORFs in Japanese and Kuril larches, and information about the ORFs could be used to elucidate flowering mechanisms and to achieve stable flower production in future studies.
In this study, we obtained 50,690 and 38,684 ORFs from cambium, needle, and shoot samples of Japanese and Kuril larches. By collecting ORFs using Iso-Seq and RNA-Seq, we constructed comprehensive reference genes for the two species. These genes were supported by high BUSCO completeness (90.5% in Japanese larch and 92.1% in Kuril larch). Our interspecific comparison revealed that the shared sequences were generally highly comprehensive and comprised of reliable ORFs. Comparisons with previously published larch genome sequences showed that the sequence collection of Japanese larch genes was comprehensive and increased our knowledge of the larch genome. The flowering signal-related ORFs were screened from the obtained ORF sequences of the two species. Furthermore, comparisons with closely related species revealed that the sequence of the flowering signal-related genes showed higher homologies to previously identified sequences from coniferous trees, indicating that the amino acid sequences of important domains are well conserved depending on their phylogenetic relationships. The isoform and RNA-Seq short-read data obtained in this study would also be useful for detecting gene loci and constructing gene models for larch genome sequences. In addition, the obtained reference sequences will provide a reference for the molecular breeding of the two larch species, and for future conifer genome evolutionary and functional genomics research.
All of the plant materials used in this study were shown in Additional File 10. Those materials were of breeding material (plus-tree clone) or genetic resources genus Larix in Japan, and clonally propagated by grafting. The differences in age of the trees shown in Additional File 10 indicate differences in the number of years elapsed since the original trees were propagated by grafting. All of the materials employed in this study are preserved as clonally propagated trees at either the Tohoku or Hokkaido Regional Breeding Office, Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization in Iwate or Hokkaido prefectures, Japan. For the sampling method, we sampled from those grafted trees to collect as many genes as possible from various organs at different timings for the comprehensiveness of the gene collection. The timing of sampling and each organs are shown in Additional File 10.
Full-length (FL) isoform sequencing
To construct the EST libraries, we sampled the branches, cambium region, and needles throughout the annual season of a 56-year-old Japanese larch plus-tree clone from Takizawa, Iwate Prefecture (Additional File 10). Cambium tissue samples were collected from the trunk at breast height (approximately 1.2–1.3-m height) and branches were randomly sampled from the tree crown. Needle samples were collected from short shoots on the branches. The same tissues were collected from a 57-year-old Kuril larch plus-tree clone from Ebetsu, Hokkaido Prefecture (Additional File 10). Total RNA was isolated using an RNeasy Plant Mini kit (QIAGEN, Gaithersburg, MD, USA) and Maxwell® RSC plant RNA kit (Promega, Madison, WI, USA). The amount and quality of total RNA were assessed using a NanoDrop 2000 (Thermo Scientific, USA) and Agilent Bioanalyzer 2100 system (Agilent Technologies, Palo Alto, CA, USA). Only high-quality total RNA extracts with RNA integrity numbers (RINs) > 7.0 were selected for analysis and extracted RNA from each organ was bulked for RNA library construction. The sequencing libraries were then prepared for isoform sequencing (Iso-Seq™) using a Clontech SMARTer PCR cDNA Synthesis Kit and a BluePippin™ Size-Selection System (Sage Science, Inc., Beverly, MA, USA) according to the manufacturer’s instructions. Briefly, the cDNA for the library was constructed as per the Clontech SMARTer-PCR cDNA Synthesis Sample Preparation Guide. Libraries of 1–2 kb, 2–3 kb, 3–6 kb, and 5–10 kb from Japanese larch and 1–2 kb, 2–3 kb, 3–4 kb, and 4–10 kb from Kuril larch were selected using the BluePippin™ Size-Selection System (Sage Science, Inc.), purified, and end-repaired before the blunt-end SMRTbell adapters were ligated. The libraries were quantified using Quant-IT PicoGreen (Invitrogen, Waltham, MA, USA) and qualified using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies, USA). Subsequent sequencing was performed in 9 and 10 SMRT Cells using P6C4 in PacBio RSII for Japanese and Kuril larches, respectively. In the obtained ROIs, full non-chimeric ROIs were clustered using the ICE software package and polished with non-full, non-chimeric ROIs using the Quiver software package . Using CD-HIT-EST v4.6.5 , the polished full non-chimeric ROIs were clustered to build collapsed redundant sequences (Additional File 11).
Construction of unigenes by short-read sequences
Cambium, needles, and branch tissues were sampled from seven trees each of Japanese and Kuril larches for RNA sequencing (Additional File 10). To collect gene sequences involved in flowering, the branch samples of GFE32200, GFE32203 (in Japanese larch), and GFF08127 (in Kuril larch) during the flower bud differentiation period, namely from June to September, were included [75, 76]. It had previously been observed that the three clones produced flowers in consecutive years. Therefore, the branch samples of these clones were thought to contain flower buds in an early development stage.
Total RNA extraction and assessments of RNA amount and quality were performed by the same procedures described above for the isoform sequences. Only RNA with RIN > 7.0 from each tree was used and extracted RNA from each organ was bulked for library construction. Using a TruSeq RNA Sample Prep kit (Illumina, Inc., San Diego, CA, USA), cDNA synthesis from the bulked RNA samples from each tree, nebulization, adaptor ligation (including index tagging for individual recognition), bridge PCR, and 101 bp paired-end sequencing were performed on Illumina HiSeq 2500/4000 platforms. The quality and adaptor trimming were performed by PRINSEQ v0.20.4  and FastX_clipper in the FASTX toolkit (http://hannonlab.cshl.edu/fastx_toolkit/), and the resultant reads were used for de novo assembly by Trinity v2.8.5 . The trimmed reads were then mapped against the contigs using Bowtie2 v184.108.40.206 , and the contigs with FPKM > 1 were filtered as “unitranscripts” to select intrinsic genes and to exclude possible contaminants. Finally, the longest unitranscripts were selected from the splicing variants in the unitranscript and defined as unigenes (Additional File 11). In the unigene assembled by short-read sequencing (RNA-Seq), the potential coding region and corresponding encoded proteins of Larix genes were identified and retrieved using TransDecoder v5.5.0 (https://github.com/TransDecoder/TransDecoder).
ORF prediction from Iso-Seq sequences
The full-length ORFs were predicted from the high-quality, non-redundant Iso-Seq sequences using the ANGEL software (https:/gthub.com/PacificBiosciences/ANGEL). Briefly, a training dataset was created using outputs from the longest ORF in all frames (Dumb ORF prediction) and training was performed using a coding potential classifier based on the obtained training data (ANGEL classifier training). Then, using this training data, ORF prediction was performed from the Iso-Seq data. The data generated by the ANGEL software were classified according to nine criteria: (i) confident-complete, where a full-length (FL) single-ORF was generated; (ii) confident-5’ partial, where a single-ORF was generated without a 3’ terminal sequence; (iii) confident-3’ partial, where a single-ORF was generated without a 5’ terminal sequence; (iv) confident-internal, where a single-ORF was generated without a start and stop codon; (v) likely-NA, where multi-ORFs were generated, and the length of only one ORF was above the threshold; (vi) suspicious-NA, where multi-ORFs were generated, and the length of some ORFs were above the threshold; (vii) dumb-complete, where a training set was generated with FL ORFs; (viii) dumb-5’ partial, where a training set was generated without a 3’ terminal sequence; (ix) dumb-3’ partial, where a training set was generated without a 5’ terminal sequence.
Consensus transcriptome construction and characterization
To construct the consensus transcriptome, the ORF sequences predicted by ANGEL and the ORFs predicted from the unigenes derived from the RNA-Seq were clustered using CD-HIT-EST v4.6.5  (Additional File 11). A metric of gene completeness for these assemblies was estimated using BUSCO v3.0  (https://busco.ezlab.org/) with the embryophyta odb10 dataset. The resulting Japanese larch ORF sequences were mapped to a published genomic sequence of Japanese larch isolate RF27 (Accession No.: WOXR00000000) using GMAP v2020.06.01 .
ORFs comparison between two larches
We used OrthoFinder v2.2.3 [82, 83] to identify orthologous and species-specific genes for the ORFs between Japanese and Kuril larches using default parameters.
Functional annotation of the ORFs
The ORFs were compared against the NCBI non-redundant protein sequence (NR) database (ftp://ftp.ncbi.nih.gov/blast/db/FASTA/nr.gz) using DIAMOND software  with the more sensitive mode. The similarities among Larix kaempferi and Larix gmelinii var. japonica were compared using BLASTP. Similarities to the protein sequences of Arabidopsis thaliana (Araport11 201606pep, https://www.araport.org), Abies sachalinensis (TodoFirGenes [http://plantomics.mind.meiji.ac.jp/todomatsu/]), Pinus lambertiana (TreeGenes PILA.1_5 peptides), Populus trichocarpa (Ensembl v4.1 proteins), and Cryptomeria japonica (MSSID: IABU01000001-01034731) were identified using BLASTP (Additional File 3, 4) with an E-value cutoff of 1e-10. The similarity search against the NR database (ftp://ftp.ncbi.nih.gov/blast/db/FASTA/nr.gz) in xml format was applied to GO assignments by BLAST2GO v4.0 . The ORFs annotated to MADS-box, CO, FLO/LFY-like, and FTL/MFT genes in Japanese and Kuril larches were aligned against those of the other plant species obtained from NCBI’s GenBank using the ClustalW module implemented in the Geneious software program  (https://www.geneious.com) with the sequences of other species retrieved from NCBI’s GenBank. The phylogenetic relationships among these genes were inferred using the neighbor-joining method with bootstrap analysis (10,000 replicates) to assess the support for each branch.
Availability of data and materials
The datasets generated in this study are available at the INSDC (DDBJ, EMBL, and GenBank) under the BioProject, PRJDB11621 (Iso-Seq and RNA-Seq; DRA011937, ORFs; ICRN01000001-01,050,690 (Japanese larch) and ICRM01000001-01,038,684 (Kuril larch)) (https://www.ncbi.nlm.nih.gov/bioproject/PRJDB11621, https://www.ncbi.nlm.nih.gov/nuccore/). The protein sequences of MADS-box, LEAFY/NEEDLY, FT/FT-like, MFT, and CONSTANS applied to the phylogenetic analyses were downloaded from the protein database in NCBI (https://www.ncbi.nlm.nih.gov/protein/). All of the materials employed in this study are preserved in the Tohoku and Hokkaido Regional Breeding Office, Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization in Iwate and Hokkaido prefectures, Japan.
National Center for Biotechnology Information
Open reading frames
Read of inserts
Ojeda DI, Mattila TM, Ruttink T, Kujala ST, Kärkkäinen K, Verta JP, et al. Utilization of tissue ploidy level variation in de Novo Transcriptome Assembly of Pinus sylvestris. G3. 2019;9:3409–21.
Nave LE, Swanston CW, Mishra U, Nadelhoffer KJ. Afforestation effects on soil carbon storage in the united states: a synthesis. Soil Sci Soc Am J.2012. https://doi.org/10.2136/sssaj2012.0236.
Chen LF, He ZB, Zhu X, Du J, Yang JJ, Li J. Impacts of afforestation on plant diversity, soil properties, and soil organic carbon storage in a semi-arid grassland of northwestern China. CATENA. 2016;147:300–7.
Doelman JC, Stehfest E, van Vuuren DP, Tabeau A, Hof AF, Braakhekk MC, et al. Afforestation for climate change mitigation: Potentials, risks and trade-offs. Glob Change Biol. 2020;26:1576–91.
Pinaceae FA. Drawings and descriptions of the genera Abies, Cedrus, Pseudolarix, Keteleeria, Nothotsuga, Tsuga, Cathaya, Pseudotsuga, Larix and Picea. Germany: Koeltz Scientific Books; 1990. p. 193–219.
Forest Agency, Ministry of Agriculture, Forest and Fisheries, Japan. Annual report on forest and forestry in Japan: Fiscal year 2019 (summary). p33. Edited by: Forestry agency. https://www.rinya.maff.go.jp/j/kikaku/hakusyo/r1hakusyo/attach/pdf/index-1.pdf
Sato M, Seki K, Kita K, Moriguchi Y, Hashimoto M, Yunoki K, et al. Comparative analysis of diterpene composition in the bark of the hybrid larch F1, Larix gmelinii var. japonica × L. kaempferi and their parent trees. J Wood Sci. 2009;55:32–40.
Kita K, Sugai T, Fujita S, Koike T. Breeding effort on hybrid larch F1 and its responses to environmental stresses. Shinrinidenikusyu. 2018;7:107–14 (in Japanese with English abstract).
Seki K, Orihashi K, Saito N, Kita K, Nakata K. Relationship between the composition and distribution of nutritional substances, secondary metabolites, and internal secretory structures in the bark tissues of Larix gmelinii var. japonica, L.kaempferi, and their F1 hybrid and susceptibility to vole herbivory. J For Res. 2019;24:292–302.
International Rice Genome Sequencing Project. The map-based sequence of the rice genome. Nature. 2005;436:793–800.
The tomato genome Consortium. The tomato genome sequence provides insights into fleshy fruit evolution. Nature. 2012;485:635–41.
Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, et al. Genome sequence of the palaeopolyploid soybean. Nature. 2010;2010(463):178–83.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, et al. The B73 Maize Genome: Complexity, Diversity, and Dynamics. Science. 2009;326:1112–5.
Nystedt B, Street NR, Wetterbom A, Zuccolo A, Lin YC, Scofield DG, et al. The Norway spruce genome sequence and conifer genome evolution. Nature. 2013;497:580–4.
Neal DB, Wegrzyn JL, Stevens KA, Zimin AV, Puiu D, Crepeau MW, et al. Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies. Genome Biol. 2014;15:R59.
Stevens KA, Wegrzyn JL, Zimin A, Puiu D, Crepeau M, Cardeno C, et al. Sequence of the Sugar Pine Megagenome. Genetics. 2016;204:1613–26.
Neale DB, McGuire PE, Wheeler NC, Stevens KA, Crepeau MW, Cardeno C, et al. The Douglas-Fir genome sequence reveals specialization of the photosynthetic apparatus in Pinaceae. G3. 2017;7:3157–67.
Niu S, Li J, Bo W, Yang W, Zuccolo A, Giacomello S, et al. The Chinese pine genome and metylome unveil key features of conifer evolution. Cell. 2022;185:1–14.
Kuzumin DA, Feranchuk SI, Sharov VV, Cybin AN, Makolov SV, Putintseva YA, et al. Stepwise large genome assembly approach: a case of Siberian larch (Larix sibirica Leded). BMC Informatics. 2019;20:37.
Neal DB, Zimin AV, Zaman S, Scott AD, Shrestha B, Workman RE, et al. Assembled and annotated 26.5Gbp coast redwood genome: a resource for estimating evolutionary adaptive potential and investigating hexaploid origin. G3. 2022;12(1):jkab380.
Sun C, Xie YH, Li Z, Liu YJ, Sun XM, Li JJ, et al. The Larix kaempferi genome reveals new insights into wood properties. J Integr Plant Biol. 2022;64:1364–73.
Hiraoka Y, Fukatsu E, Mishima K, Hirao T, Teshima MK, Tamura M, et al. A Potential of genome-wide studies in unrelated plus trees of a coniferous species, Cryptomeria japonica (Japanese cedar). Front Plant Sci. 2018;9:1322.
Mishima K, Hirao T, Tsubomura M, Tamura M, Kurita M, Nose M, et al. Identification of novel putative causative genes and genetic marker for male sterility in Japanese cedar (Cryptomeria japonica D.Don). BMC Genomics. 2018;19:277.
Beaulieu J, Doerksen T, Clément S, MacKay J, Bousquet J. Accuracy of genomic selection models in a large population of open-pollinated families in white spruce. Heredity. 2014;113:343–52.
Pavy N, Gagnon F, Rigault P, Blais S, Deschenes A, Boyle B, et al. Development of high-density SNP genotyping arrays for white spruce (picea glauca) and transferability to subtropical and nordic congeners. Mol Ecol Res. 2013;13:324–36.
Baison J, Vidalis A, Zhou L, Chen ZQ, Li Z, Sillanpää MJ, et al. Genome-wide association study identified novel candidate loci affecting wood formation in Norway spruce. Plant J. 2019;100:83–100.
Baison J, Zhou L, Forsberg N, Mörling T, Grahn T, Olsson L, et al. Genetic control of tracheid properties in Norway spruce wood. Sci Rep. 2020;10:18089.
Hirao T, Matsunaga K, Hirakawa H, Shirasawa K, Isoda K, Mishima K, et al. Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii). BMC Plant Biol. 2019;19:424.
Eckert AJ, Wegrzyn JL, Liechty JD, Lee JM, Cumbie WP, Davis JM, et al. The Evolutionary genetics of the genes underlying phenotypic associations for Loblolly Pine (Pinus taeda, Pinaceae). Genetics. 2013;195:1353–72.
Resende MFR Jr, Muñoz P, Resende MDV, Garrick DJ, Fernando RL, Davis JM, et al. Accuracy of genomic selection methods in a standard data set of Loblolly Pine (Pinus taeda L). Genetics. 2012;190:1503–10.
Neves LG, Davis JM, Barbazuk WB, Kirst M. A high-density gene map of Loblolly Pine (Pinus taeda L.) based on exome sequence capture genotyping. G3. 2014;4:29–37.
Cai Q, Li B, Lin F, Huang P, Guo W, Zheng Y. De novo sequencing and assembly analysis of transcriptome in Pinus bungeana Zucc. ex Endl. Forests. 2018;9:156.
Ueno S, Nakamura Y, Kobayashi M, Terashima S, Ishizuka W, Uchiyama K, et al. TodoFirGene: Developing Transcriptome Resources for Genetic Analysis of Abies sachalinensis. Plant Cell Physiol. 2018;59:1276–84.
Zhao YJ, Cao Y, Wang J, Xiong Z. Transcriptome sequencing of Pinus kesiya var. langbianensis and comparative analysis in the Pinus phylogeny. BMC Genomics. 2018;19:725.
Kuang X, Sun S, Wei J, Li Y, Sun C. Iso-Seq analysis of the Taxus cuspidata transcriptome reveals the complexity of Taxol biosynthesis. BMC Plant Biol. 2019;19:210.
Li WF, Yang WH, Zhang SG, Han SY, Qi LW. Transcriptome analysis provides insights into wood formation during larch tree aging. Tree Genet Genomes. 2017;13:19.
Denoeud F, Aury JM, Da Silva C, Noel B, Rogier O, Delledonne M, et al. Annotating genomes with massive-scale RNA sequencing. Genome Biol. 2008;9:R175.
Li B, Fillmore N, Bai Y, Collins M, Thomson JA, Stewart R, et al. Evaluation of de novo transcriptome assemblies from RNA-Seq data. Genome Biol. 2014;15:553.
Feng S, Xu M, Liu F, Cui C, Zhou B. Reconstruction of the full-length transcriptome atlas using PacBio Iso-Seq provides insight into the alternative splicing in Gossypium australe. BMC Plant Biol. 2019;19:365.
Rhoads A, Au KF. PacBio Sequencing and Its Applications. Genomics Proteomics Bioinformatics. 2015;13:278–89.
Yanagihara T, Tochiaki K, Arai K. On the relation between the harvest of Japanese larch seed and meteorological conditions. J Jpn For Soc. 1960;42:347–51 (in Japanese with English abstract).
Morse AM, Peterson DG, Islam-Faridi MN, Smith KE, Magbanua Z, Garcia SA, et al. Evolution of Genome Size and Complexity in Pinus. PLoS One. 2009;4:e4332.
Kovach A, Wegrzyn JL, Parra G, Holt C, Bruening GE, Loopstra CA, et al. The Pinus taeda genome is characterized by diverse and highly diverged repetitive sequences. BMC Genomics. 2010;11:420.
Wegrzyn JL, Liechty JD, Stevens KA, Wu LS, Loopstra CA, Vasquez- Gross HA, et al. Unique Features of the Loblolly Pine (Pinus taeda L.) Megagenome Revealed Through Sequence Annotation. Genetics. 2014;196:891–909.
Li WF, Kang Y, Zhang Y, Zang QL and Qi LW. Concerted control of the LaRAV1-LaCDKB1;3 module by temperature during dormancy release and reactivation of larch. Tree Physiol. 2021;41:1918-37.
Lee J, Lee I. Regulation and function of SOC1, a flowering pathway integrator. J Exp Bot. 2010;61:2247–54.
Winter KU, Becker A, Münster T, Kim JT, Aaedler H, Theissen G. MADS-box genes reveal that gnetophytes are more closely related to conifers than to flowering plants. Proc Natl Acad Sci USA. 1999;96:7342–7.
Tandre K, Albert VA, Sundås A, Engström P. Conifer homologues to genes that control floral development in angiosperms. Plant Mol Biol. 1995;27:69–78.
Uddenberg D, Reimegård J, Clapham D, Almqvist C, von Arnold S, Emanuelsson O, et al. Early Cone Setting in Picea abies acrocona is associated with increased transcriptional activity of a MADS Box transcription factor. Plant Physiol. 2013;161:813–23.
Walden AR, Wang DY, Walte CR, Gardner RC. A large family of TM3 MADS-box cDNAs in Pinus radiata includes two members with deletions of the conserved K domain. Plant Sci. 1998;138:167–76.
Zhang Y, Zang QL, Qi LW, Han SY, Li WF. Effects of cutting, pruning, and grafting on the expression of age-related genes in Larix kaempferi. Forests. 2020;11:218.
Katahata S, Futamura N, Igasaki T, Shinohara K. Functional analysis of SOC1-like and AGL6-like MADS-box genes of the gymnosperm Cryptomeria japonica. Tree Genet Genomes. 2014;10:317–27.
Xiang WB, Li WF, Zhang SG, Qi LW. Transcriptome-wide analysis to dissect the transcription factors orchestrating the phase change from vegetative to reproductive development in Larix kaempferi. Tree Genet Genomes. 2019;15:68.
Ma JJ, Chen X, Song YT, Zang GF, Zhou XQ, Que SP, et al. MADS-box transcription factors MADS11 and DAL1 interact to mediate the vegetative-to-reproductive transition in pine. Plant Physiol. 2021;187:247–62.
Moyroud E, Tichtinsky G, Parcy F. The LEAFY Floral Regulators in Angiosperms: Conserved Proteins with Diverse Roles. J Plant Biol. 2009;52:177–85.
Moyroud E, Kusters E, Monniaux M, Koes R, Parcy F. LEAFY blossoms. Trends Plant Sci. 2010;15:346–52.
Frohlich MW, Parker DS. The mostly male theory of flower evolutionary origins: from genes to fossils. Syst Bot. 2000;25:155–70.
Vázquez-Lobo A, Carlsbecker A, Vergara-Silva F, Alvarez-Buylla ER, Piñero D, Engström P. Characterization of the expression patterns of LEAFY/FLORICAULA and NEEDLY orthologs in female and male cones of the conifer genera Picea, Podocarpus, and Taxus: implications for current evo-devo hypotheses for gymnosperm. Evol Dev. 2007;9:446–59.
Lu Y, Ran JH, Guo DM, Yang ZY, Wang XQ. Phylogeny and divergence times of gymnosperms inferred from single-copy nuclear genes. PLoS One. 2014;9:e107679.
Moyroud E, Monniaux M, Thévenon E, Dumas R, Scutt CP, Frohlich MW, et al. A link between LEAFY and B-gene homologues in Welwitschia mirabilis sheds light on ancestral mechanisms prefiguring floral development. New Phytol. 2017;216:469–81.
Carlsbecker A, Tandre K, Johanson U, Englund M, Engström P. The MADS-box gene DAL1 is a potential mediator of the juvenile-to-adult transition in Norway spruce (Picea abies). Plant J. 2004;40:546–57.
Mouradov A, Glassick T, Hamdorf B, Murphy L, Fowler B, Marla S, et al. NEEDLY, a Pinus radiata ortholog of FLORICAULA/LEAFY genes, expressed in both reproductive and vegetative meristems. Proc Natl Acad Sci USA. 1998;95:6537–42.
Abe M, Kobayashi Y, Yamamoto S, Daimon Y, Yamaguchi A, Ikeda Y, et al. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science. 2005;309:1052–6.
Wigge PA, Kim MC, Jaeger KE, Busch W, Schmid M, Lohmann JU, et al. Integration of spatial and temporal information during floral induction in Arabidopsis. Science. 2005;2005(309):1056–9.
Kaufmann K, Wellmer F, Muiño JM, Ferrier T, Wuest SE, Kumar V, et al. Orchestration of floral initiation by APETALA1. Science. 2010;2010(328):85–9.
Karlgren A, Gyllenstrand N, Källman T, Sundström JF, Moore D, Lascoux M, et al. Evolution of the PEBP gene family in plants: functional diversification in seed plant evolution. Plant Physiol. 2011;156:1967–77.
Gyllenstrand N, Clapham D, Källman T, Lagercrantz U. A Norway Spruce FLOWERING LOCUS T homolog is implicated in control of growth rhythm in conifers. Plant Physiol. 2007;144:248–57.
Karlgren A, Gyllenstrand N, Clapham D, Lagercrantz U. FLOWERING LOCUS T/TERMINAL FLOWER1-Like genes affect growth rhythm and bud set in Norway Spruce. Plant Physiol. 2013;163:792–803.
Avia K, Kärkkäinen K, Lagercrantz U, Savolainen O. Association of FLOWERING LOCUS T/TERMINAL FLOWER 1-like gene FTL2 expression with growth rhythm in Scots pine (Pinus sylvestris). New Phytol. 2014;204:159–70.
Yan J, Mao D, Liu X, Wang L, Xu F, Wang G, et al. Isolation and functional characterization of a circadian-regulated CONSTANS homolog (GbCO) from Ginkgo biloba. Plant Cell Rep. 2017;36:1387–99.
Turck F, Fornara F, Coupland G. Regulation and identity of florigen: FLOWERING LOCUS T moves center stage. Annu Rev Plant Biol. 2008;59:573–94.
Holefors A, Opseth L, Rosnes AKR, Ripel L, Snipen L, Fossdal CG, et al. Identification of PaCOL1 and PaCOL2, two CONSTANS-like genes showing decreased transcript levels preceding short day induced growth cessation in Norway spruce. Plant Physiol Biochem. 2009;47:105–15.
Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods. 2013;10:563–9.
Li W, Godzik A. Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006;22:1658–9.
Hashizume H, Imai M. On developmental process of flower buds in Larix leptolepis. J Jpn For Sci. 1966;48:425–35 (in Japanese with English abstract).
Uciyama K, Kromaru M, Kita K. Flower bud development and prediction of the number of cones in Larix gmelinii var. japonica. For. Tree. Breed. 2008;227: 11–6. (in Japanese with English abstract)
Schmieder R, Edwards R. Quality control and preprocessing of metagenomic datasets. Bioinformatics. 2011;27:863–4.
Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, et al. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature Protoc. 2013;8:1494–512.
Langmead B, Salzberg S. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012;9:357–9.
Simão FA, Waterhouse RM, Ioannidis P, Kriventseva EV, Zdobnov EM. (2015) BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics. 2015;31:3210–2.
Wu TD, Watanabe CK. GMAP: a genomic mapping and alignment program for mRNA and EST sequences. Bioinformatics. 2005;21:1859–75.
Emms DM, Kelly S. OrthoFinder: solving fundamental biases in whole genome comparisons dramatically improves orthogroup inference accuracy. Genome Biol. 2015;16:157.
Emms DM, Kelly S. (2019) OrthoFinder: phylogenetic orthology inference for comparative genomics. Genome Biol. 2019;20:238.
Buchfink B, Xie C, Huson DH. Fast and sensitive protein alignment using DIAMOND. Nat Methods. 2015;12:59–60.
Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005;21:3674–6.
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, et al. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012;28:1647–9.
We thank Dr. Soichiro Nagano for kindly providing helpful comments.
This study was supported in part by grants from the NARO Bio-oriented Technology Research Advancement Institution (a special project on regional development strategy; Forestry C105), MAFF commissioned project study on “Development of efficient breeding technique aiming at forestry trees with superior carbon storage capacity” (Grant Number JPJ009841), and the Kazusa DNA Research Institute Foundation.
Ethics approval and consent to participate
This study did not involve humans, vertebrates and plant species at risk of extinction, and the materials employed in this study are preserved at our institute. All experimental research in this study complied with the IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora. As researchers of the Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization, we are allowed to use these forest trees as research materials.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1.
Stacked percent of the ORFs with top hits against NCBI nr database in each region on the Venn diagram. Blue shows the plant and fungal species (gbpln) in the GenBank nucleotide divisions. Orange shows ratio of “no hit”. Gray showed ratio of other divisions. LK and LG shows Japanese and Kuril larch, respectively.
Additional file 2.
Gene ontology (GO) categories in biological process (BP) that encoded proteins with sequence similarity (E-value ≤ 1e-10) in the NCBI database. a) Japanese larch, b) Kuril larch. Gene ontology (GO) categories in cellular component (CC) that encoded proteins with sequence similarity (E-value ≤ 1e-10) in the NCBI database. a) Japanese larch, b) Kuril larch. Gene ontology (GO) categories in molecular function (MF) that encoded proteins with sequence similarity (E-value ≤ 1e-10) in the NCBI database. a) Japanese larch, b) Kuril larch.
Additional file 3.
Description of Japanese larch used in this study.
Additional file 4.
Description of Kuril larch used in this study.
Additional file 5.
Number of open reading frames (ORFs) similar to NCBI sequeces and other species according to BLASTp E-value cutoff values. a) ORFs obtained from Japanese larch, b) ORFs obtained from ORF Kuril larch.
Additional file 6.
Alignment of known MADS-box genes and a set of other angiosperm and gymnosperm sequences. Japanese larch open reading frames are shown in green. Kuril larch open reading frames are shown in blue.
Additional file 7.
Alignment of known LEAFY and NEEDLY genes and a set of other angiosperm and gymnosperm sequences. Japanese larch open reading frames are shown in green. Kuril larch open reading frames are shown in blue.
Additional file 8.
Alignment of known FT/FT-like and MFT genes and a set of other angiosperm and gymnosperm sequences. Japanese larch open reading frames are shown in green. Kuril larch open reading frames are shown in blue.
Additional file 9.
Alignment of known CONSTANS genes and a set of other angiosperm and gymnosperm sequences. Japanese larch open reading frames are shown in green. Kuril larch open reading frames are shown in blue.
Additional file 10.
List of samples collected in this study.
Additional file 11.
Summary of assembly and characterization.
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Mishima, K., Hirakawa, H., Iki, T. et al. Comprehensive collection of genes and comparative analysis of full-length transcriptome sequences from Japanese larch (Larix kaempferi) and Kuril larch (Larix gmelinii var. japonica). BMC Plant Biol 22, 470 (2022). https://doi.org/10.1186/s12870-022-03862-9
- Larix kaempferi
- L. gmelinii var. japonica
- Isoform sequencing
- Short-read sequences
- Flowering signal-related genes