Table 7 Statistics and completeness of merged ORFs from Iso-seq and RNA-Seq short-reads
| Â | Larix kaempferi | Larix gmelinii var. japonica | ||
|---|---|---|---|---|
|  | Merged ORFs (Isoseq + RNA seq) | Merged ORFs after clustering (Accession No.: ICRN01000001-1050690) | Merged ORFs (Isoseq + RNA seq) | Merged ORFs after clustering (Accession No.: ICRM01000001-1038684) |
Number of ORFs | 107,687 | 50,690 | 87,539 | 38,684 |
Total length (bases) | 136,244,546 | 53,804,534 | 101,368,555 | 39,053,584 |
Average (bases) | 1,265 | 1,061 | 1,158 | 1,010 |
Maximum (bases) | 14,493 | 14,493 | 14,493 | 14,493 |
Minimum (bases) | 145 | 145 | 146 | 146 |
N50 (bases) | 1,665 | 1,473 | 1,539 | 1,413 |
G + C% | 44.8 | 46.4 | 44.5 | 45.1 |
A | 39,163,057 | 15,014,796 | 29,209,051 | 11,107,507 |
T | 35,977,224 | 13,798,508 | 27,009,101 | 10,313,639 |
G | 33,156,639 | 13,227,788 | 24,804,297 | 9,554,898 |
C | 27,947,626 | 11,763,442 | 20,346,106 | 8,077,540 |
Category of ANGEL | ||||
 Confident | - | 14,264 | - | 120,23 |
  Confident- complete | - | 8,933 | - | 8,315 |
  Confident-5' partial | - | 5,174 | - | 3,589 |
  Confident-3' partial | - | 122 | - | 105 |
  Confident- internal | - | 35 | - | 14 |
 Likely-NA | - | 4,143 | - | 3,142 |
 Suspicious-NA | - | 6,991 | - | 6,611 |
 Dumb-complete | - | 7,126 | - | 5,644 |
 Dumb-5'partial | - | 6 | - | 3 |
 Dumb-3'partial | - | 80 | - | 67 |
Category of Transdecorder | ||||
 Complete | - | 10,099 | - | 6,489 |
 5' Prime_partial | - | 2,946 | - | 1,552 |
 3' Prime_partial | - | 1,885 | - | 1,023 |
 Internal | - | 3,150 | - | 2,130 |
BUSCO v3 (odb10; 1375) | ||||
 Complete (%) | 90.6 | 90.5 | 92.1 | 92.1 |
  Complete and single-copy (%) | 39.8 | 83.3 | 32.8 | 84.8 |
  Complete and duplicated (%) | 50.8 | 7.2 | 59.3 | 7.3 |
 Fragmented (%) | 3.1 | 3 | 1.5 | 1.5 |
 Missing (%) | 6.3 | 6.5 | 6.4 | 6.0 |