SiLEA14, a novel atypical LEA protein, confers abiotic stress resistance in foxtail millet
© Wang et al.; licensee BioMed Central Ltd. 2014
Received: 30 May 2014
Accepted: 15 October 2014
Published: 18 November 2014
Late embryogenesis abundant (LEA) proteins are involved in protecting higher plants from damage caused by environmental stresses. Foxtail millet (Setaria italica) is an important cereal crop for food and feed in semi-arid areas. However, the molecular mechanisms underlying tolerance to these conditions are not well defined.
Here, we characterized a novel atypical LEA gene named SiLEA14 from foxtail millet. It contains two exons separated by one intron. SiLEA14 was expressed in roots, stems, leaves, inflorescences and seeds at different levels under normal growth conditions. In addition, SiLEA14 was dramatically induced by osmotic stress, NaCl and exogenous abscisic acid. The SiLEA14 protein was localized in the nucleus and the cytoplasm. Overexpression of SiLEA14 improved Escherichia coli growth performance compared with the control under salt stress. To further assess the function of SiLEA14 in plants, transgenic Arabidopsis and foxtail millet plants that overexpressed SiLEA14 were obtained. The transgenic Arabidopsis seedlings showed higher tolerance to salt and osmotic stress than the wild type (WT). Similarly, the transgenic foxtail millet showed improved growth under salt and drought stresses compared with the WT. Taken together, our results indicated that SiLEA14 is a novel atypical LEA protein and plays important roles in resistance to abiotic stresses in plants.
We characterized a novel atypical LEA gene SiLEA14 from foxtail millet, which plays important roles in plant abiotic stress resistance. Modification of SiLEA14 expression may improve abiotic stress resistance in agricultural crops.
Environmental stresses, such as drought and high salinity, can cause severe damage to plants, leading to considerable reduction in their productivity. To survive under such conditions, plants have developed a series of defense responsive pathways. Among them, Ca2+-dependent signaling leads to the activation of late embryogenesis abundant (LEA)-type genes, which may function in protection and damage repair of plants .
LEA proteins were first identified in cotton seeds . The proteins accumulated to high levels in the late stages of seed development ,. Subsequently, they were found to be expressed in vegetative tissues , and could be induced by abscisic acid (ABA) and various abiotic stresses, such as drought and cold -. With the development of deep sequencing technology, an increasing number of LEA proteins have been identified. On the basis of their amino acid sequence similarities and conserved motifs, LEA proteins are categorized into different groups -. In this work, we adopt the classification introduced by Battaglia’s group, in which LEA proteins are categorized into seven distinct families . Groups 1, 2, 3, 4, 6 and 7, which share specific motifs within each group, are considered to be hydrophilic or “typical” LEA proteins. Conversely, group 5 corresponds to atypical LEA proteins. This group includes all LEA proteins with higher content of hydrophobic residues than typical LEA proteins. On the basis of their sequence similarity, group 5 LEA proteins are divided into the subgroups 5A, 5B, and 5C, corresponding to the first described proteins D-34, D-73, and D-95 , in this group, respectively. Physicochemical properties show that group 5 LEA proteins are not soluble after boiling, suggesting that they may adopt a globular conformation and are not heat stable ,,. Subsequent reports show that subgroup 5C LEA proteins are natively folded and have more β-sheets than α-helixes ,, which is different from subgroups 5A and 5B LEA proteins that are intrinsically unstructured -. For example, Arabidopsis LEA14 has an αβ-fold consisting of one α-helix and seven β-strands that form two antiparallel β-sheets as determined by nuclear magnetic resonance spectroscopy . Moreover, subgroup 5C LEA proteins have other outstanding characteristics, such as lower instability index, narrower range of GRAVY values, and lower proportion of polar (hydrophilic) and small residues, but higher proportion of non-polar residues than subgroup 5A and 5B members . All of these differences indicate that subgroup 5C LEA proteins may function differently from other LEA proteins in plants.
At present, only a small number of subgroup 5C LEA genes have been characterized. Their transcripts can be upregulated in response to diverse stresses, as reported for cotton LEA14-A , Craterostigma plantagineum PcC27-45 , soybean D95-4 , tomato ER5 , hot pepper CaLEA6 , Arabidopsis LEA14  and At2g44060 , and sweetpotato IbLEA14 . Overexpression of CaLEA6 in tobacco improves tolerance to dehydration and NaCl but not to low temperature . Transgenic sweetpotato non-embryogenic calli that overexpress IbLEA14 show increased tolerance to drought and salt stress by enhancing lignification . Recently, rice OsLEA5 has been reported to enhance resistance against diverse abiotic stresses in recombinant Escherichia coli cells. In vitro analysis showed that OsLEA5 was able to protect lactate dehydrogenase from aggregation under different abiotic stresses . All these results suggest that subgroup 5C LEA proteins are closely associated with resistance to multiple abiotic stresses.
Foxtail millet (Setaria italica (L.) Beauv.), a member of the Poaceae family, has a long history in cultivation of about 7000 years. It has been widely planted in northern China and other Asian countries. Recently, a draft genome sequence for foxtail millet has been completed , which enables foxtail millet to be a tractable experimental grass model . As a diploid C4 panicoid crop species, foxtail millet is well known for its remarkable drought resistance. However, the molecular mechanisms underlying this tolerance are not well defined.
In this study, we isolated and functionally characterized a novel member of the atypical subgroup 5C LEA gene, SiLEA14, from foxtail millet. The expression of SiLEA14 was induced by ABA, polyethylene glycol (PEG) and NaCl. Overexpression of SiLEA14 resulted in enhanced resistance to abiotic stresses in E. coli, Arabidopsis and foxtail millet. The SiLEA14 promoter mediated remarkable induction of β-glucuronidase (GUS) expression in transgenic Arabidopsis under various stresses. Cis-acting regulatory elements in the SiLEA14 promoter were also predicted. These data reveal the potential application of SiLEA14 in the genetic engineering of other crops.
SiLEA14 is an atypical LEA protein
The full-length sequence of SiLEA14 was determined by 5′ and 3′ rapid amplification of cDNA ends (RACE) [GenBank: KJ767551]. The sequence is 821 bp in length, with a 100 bp 5′ untranslated region (UTR) and a 208 bp 3′ UTR (with polyA tail) (Additional file 1A). SiLEA14 harbors two exons separated by an intron (Additional file 1B), and encodes an open reading frame of 170 aa with a predicted molecular mass of 18.77 kD and pI of 5.56. It is rich in Ser (10.6%), Lys (8.8%), and Ile (8.2%), but contains low quantities of Trp (1.2%), Asn (1.8%), Cys (1.8%), and Gln (1.8%). Three hydrophobic regions (I, II, and III) were identified in the SiLEA14 protein (Additional file 1C). A motif search of the SiLEA14 protein in InterProScan revealed that it contains a “LEA_2” motif (PF03186, 3.4e-20), which was classified into subgroup 5C (D-95) according to Battaglia’s classification of LEA proteins . Further analysis showed that SiLEA14 contains a lower percentage of polar amino acids (47.2%) and higher percentage of non-polar amino acids (25.8%) than other group LEA proteins (Additional file 1D) . The GRAVY value and the instability index of SiLEA14 is −0.155 and 34.68, respectively (Additional file 1D). All these characteristics were in accordance with those of other members of subgroup 5C .
Subcellular localization of SiLEA14
SiLEA14 expression profiles under normal and stress conditions
The expression level of SiLEA14 was also examined by qRT-PCR under various stresses. Under ABA treatment, SiLEA14 transcription abruptly reached its highest level (six-fold) after 1 h treatment, and then decreased gradually and reverted almost to the control level at 24 h (Figure 3B). Under PEG stress, SiLEA14 transcription was rapidly induced within 0.5 h and increased to a peak level (8-fold) at 12 h (Figure 3C). Salt treatment resulted in increased accumulation of SiLEA14 to a maximum level (11-fold) only after 3 h (Figure 3D). These results indicated that SiLEA14 might play important roles in the responses to salt and drought stresses.
SiLEA14 enhances salt tolerance in transformed E. coli
SiLEA14 improves the abiotic stress resistance of transgenic Arabidopsis
Under non-stress conditions, no significant differences in the fresh and dry weights were observed between the WT and transgenic plants (Figure 5C and D). However, when grown on Murashige and Skoog (MS) medium supplemented with 125 mM NaCl, the transgenic seedlings showed significantly larger cotyledons and longer roots than those of the WT, even though growth of these organs of the transgenic and WT plants was inhibited compared with normal conditions (Figure 5C). Consistently, the fresh and dry weights of transgenic seedlings were significantly higher than that of the WT (Figure 5E). Similar results were obtained when seedlings were treated with 250 mM mannitol (Figure 5C and F). These results suggest that SiLEA14 may enhance the osmotic stress resistance of transgenic Arabidopsis.
Overexpression of SiLEA14 increases the salt tolerance of transgenic foxtail millet
First, we examined the salt tolerance of transgenic foxtail millet during germination, and the results were shown in Figure 6. Compared with the WT plants, transgenic lines showed better growth performance (Figure 6C) with enhanced shoot and root growth (Figure 6E and G) when germinated in water for 4 days. Under salt stress for 4 days, the transgenic lines showed significantly longer shoot and root than the WT (Figure 6E and G), even though the growth of both WT and transgenic lines were seriously suppressed (Figure 6C). Similar results were obtained for 9 days salt stress treatment (Figure 6D, F and H).
Electrolyte leakage always occurs following membrane damage under salinity stress . Furthermore, plants accumulate several metabolites, such as amino acids (e.g., proline), sugars, and sugar alcohols (e.g., mannitol and trehalose), to prevent detrimental changes caused by severe osmotic stress ,. Therefore, we measured the electrolyte leakage and change in free proline and soluble sugar contents in control and SiLEA14 transgenic lines with or without salinity stress, respectively. Under optimal or salt-stress conditions, the amount of electrolyte leakage in the transgenic lines was significantly lower than that of the WT plants (Figure 7B). This finding is in agreement with the result that the SiLEA14 transgenic lines showed enhanced germination and growth compared with the WT under both normal and salt-stress conditions (Figures 6C h, 7A). No significant differences in free proline and soluble sugar contents were observed between the WT and the transgenic lines under normal conditions. Under 150 and 250 mM NaCl stress, the free proline and soluble sugar contents increased in all plants. However, this increase was significantly more pronounced in the transgenic lines than in the WT plants (Figure 7C, D). All of these increased osmotic protectants are beneficial for protection of the plants against salt stress. Moreover, we noted that the transgenic line L68 demonstrated better salt tolerance than the other two transgenic lines (L76 and L78), which correlated with the higher accumulation of SiLEA14 transcripts in L68. All of these results indicated that SiLEA14 overexpressing foxtail millet is more tolerant to salt stress compared with the WT.
Overexpression of SiLEA14 increases drought resistance of transgenic foxtail millet
We also examined the drought resistance of transgenic foxtail millet during germination. When germinated in water for 4 days, the transgenic lines showed better growth performance than WT as described above (Figure 6C, E and G). Compared with in water, no obvious suppression under 10% PEG stress but serious suppression under 20% PEG stress for 4 days were observed (Figure 6C). The shoot and root length of transgenic lines was longer than that of the WT no matter germinated in water, 10% or 20% PEG (Figure 6E and G). When germinated in water or PEG for 9 days, similar results were obtained (Figure 6D, F and H).
The change in free proline and soluble sugar contents in control and SiLEA14 transgenic lines with or without drought stress was examined. No significant differences in free proline and soluble sugar contents were observed between the WT and the transgenic lines under non-stress conditions. Under drought stress for 3 days, the free proline and soluble sugar contents increased in all WT and transgenic plants. However, a significantly higher increase in free proline content was observed in the transgenic lines L68 and L78 when compared to the WT. In addition, compared with the WT, the soluble sugar content increased significantly in the transgenic lines L68 and L76 (Figure 8C, D). Taken together these results indicate that SiLEA14 overexpressing foxtail millet showed improved drought resistance.
GUS activity is induced by various stresses in proSiLEA14::GUS transgenic Arabidopsis
SiLEA14 promoter contains stress-associated cis-elements
Putative cis- acting regulatory elements associated with ABA and various stresses in SiLEA14 promoter region
Cis -element name
−793, −792, −133, −78, −77
ABA-mediated regulation of transcription; Required for etiolation-induced expression of erd1 in Arabidopsis
ABRE element involved in Arabidopsis dehydration-responsive gene rd22
−792, −382, −132, −77
Involved in ABA response; Required for etiolation-induced expression of erd1 in Arabidopsis
DBF1 and DBF2 binding site in the maize rab17 gene promoter involved in ABA induction
DRE/CRT regulatory element involved in dehydration, cold or sanility responsiveness
Binding site of barley CBF1 and CBF2 involved in cold acclimation
MYC binding site in rd22 gene of Arabidopsis; ABA-induction
−1034, −867, −793, −666, −588, −553, −485, −468, −433, −78
MYC recognition site in rd22, CBF3 and many other genes in Arabidopsis; Binding site of ICE1 that regulates the transcription of CBF/DREB1 genes in the cold in Arabidopsis
MYC recognition sequence necessary for expression of erd1 in dehydrated Arabidopsis; Binding site of NAC which is stress-inducible
−468, −465, −360
MYB binding site involved in regulation of genes that are responsive to water stress in Arabidopsis
MYB recognition sequence found in the promoters of rd22 and many other genes in Arabidopsis
Typical LEA proteins can retain water molecules and protect other proteins from aggregation or desiccation because of their highly hydrophilic properties . Conversely, atypical LEA proteins have higher content of hydrophobic residues than typical LEAs. The latter have been speculated to be involved in diverse stress tolerances, although few studies have been carried out to characterize their functions ,,. Here, we reported the identification and characterization of SiLEA14, a novel atypical LEA member, in foxtail millet.
As a key phytohormone, ABA plays an important role in plant stress responses. Osmotic stress-regulated genes can be activated through both ABA-dependent and ABA-independent pathways . However, it is considered that stress-signalling pathways for the activation of LEA-like genes completely independent of ABA may not exist . In our study, SiLEA14 accumulation was remarkably induced by ABA and peaked rapidly after 1 h treatment (Figure 3). In addition, SiLEA14 promoter-driven GUS activity was distinctly stimulated by ABA (Figure 9). Compared with the WT, transgenic foxtail millet seeds showed better germination in ABA solution (Figure 6C–H). All of these results indicate that activation of SiLEA14 under salt and drought stresses may be dependent on ABA.
Yeast and E. coli heterologous systems have been widely used to investigate LEA gene functions ,,. In the present study, overexpression of SiLEA14 protected E. coli cells from damage caused by salt stress (Figure 4). Overexpression of SiLEA14 in Arabidopsis imparted increased tolerance to salt and mannitol stresses (Figure 5). This result suggests that SiLEA14 from foxtail millet, a monocot, can function properly in the dicot Arabidopsis. Interestingly, transgenic foxtail millet overexpressing SiLEA14 exhibited superior germination and subsequent growth in soil compared with the WT even under normal conditions. Under salt and drought stresses, these differences were more remarkable, which were indicative of the key roles of SiLEA14 in foxtail millet (Figures 6, 7 and 8). Consistent with these findings, both SiLEA14 transcription and SiLEA14 promoter-driven GUS activity were remarkably induced by NaCl and PEG (Figures 3, 9 and Additional file 5). However, it should be noted that some discrepancies were observed between the expression pattern of the endogenous SiLEA14 upon abiotic stress and ABA treatment (Figure 3) and the SiLEA14 promoter-driven GUS transcript accumulation responsive to abiotic stress and ABA driven by SiLEA14 promoter region in transgenic Arabidopsis (Figure 9). For example, the SiLEA14 transcripts accumulated to the highest level after 250 mM NaCl treatment for 3 h (Figure 3), whereas the GUS transcripts reached the peak after 6 h treatment (Figure 9). Additionally, the histochemical analysis showed strong GUS signals in the petioles of transgenic Arabidopsis (Additional file 5), whereas the endogenous SiLEA14 was highly expressed in roots (Figure 3). This is probably due to that SiLEA14 is from the monocot foxtail millet and the GUS expression driven by SiLEA14 promoter is performed in the dicot Arabidopsis.
So far, there is only one report on the functional mechanism of the atypical LEA protein in plants . Overexpression of IbLEA14, a homologous gene of SiLEA14, enhanced tolerance to drought and salt stress in the sweetpotato calli. The contents of lignin in the IbLEA14-overexpressing calli were increased under normal conditions. The authors inferred that IbLEA14 may be involved in these functions as a consequence of regulating increased lignin production. In our study, we found that overexpression of SiLEA14 in Arabidopsis and foxtail millet obviously improved the osmotic stress resistance of transgenic plants. Meanwhile, the proline and sugar accumulated at a higher level in transgenic lines, especially in L68 which accumulated higher SiLEA14 transcripts, than WT after osmotic and NaCl stress. These results implied that overexpression of SiLEA14 might up-regulate these metabolites. However, further study is needed.
The identification of cis-acting elements in the SiLEA14 promoter may help to provide insight into the molecular mechanism of SiLEA14 function. As a major cis-acting element, ABRE has been identified in the promoters of many ABA-inducible genes of plants such as the cotton LEA gene D-113 . The bZIP transcription factors AREB/ABF can bind to ABRE and activate ABA-inducible gene expression -. In the present study, ten cis-elements containing the ABRE ACGT-core were identified in the −793 to −77 bp region of the SiLEA14 promoter. The DRE element was first identified in the promoter of rd29A, a gene responsive to dehydration stress in Arabidopsis . In plants, the AP2 transcription factors DREB/CBF specifically bind to the DRE element to regulate the expression of the downstream stress-responsive genes . There are interactions between ABRE and DRE in Arabidopsis rd29A gene expression in response to dehydration and high-salinity stresses . One DRE-like element was predicted in the SiLEA14 promoter. In Arabidopsis, an MYC transcription factor, AtMYC2 and an MYB transcription factor, AtMYB2, have been shown to bind the cis-elements CANNTG and C/TAACNA/G, respectively, to regulate expression of the dehydration-responsive gene RD22 ,. Ten MYC and three MYB-like sequences were identified in the SiLEA14 promoter. Proteins that bind to these elements remain to be isolated. It is necessary to further elucidate the detailed functions of these putative regulatory cis-elements. In addition, pathogen-related elements were also identified. However, whether SiLEA14 participates in biotic stress responses needs to be investigated.
In conclusion, this study characterizes a novel atypical LEA gene SiLEA14 from foxtail millet. SiLEA14 is responsive to ABA, PEG and NaCl and the SiLEA14 is localized in the cytosol. SiLEA14 improves the salt tolerance of E. coli transformant and transgenic Arabidopsis. Furthermore, overexpression of SiLEA14 significantly enhances the salt and drought tolerances of transgenic foxtail millet. SiLEA14 plays important roles in plant abiotic stress resistance and could be used in crops genetic engineering with the aim of improving stress tolerance.
Plant materials and growth conditions
Seeds of foxtail millet (Setaria italica cv. Jigu11), kindly provided by Prof. Xianmin Diao of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, China, were geminated on moist gauze for 24 h at 30°C, and then grown in pots filled with nutrient soil and vermiculite mixed at 1:1 (v/v) in the controlled chamber (25–26°C, humidity 60-70%, under 16-h light/8-h darkness). Foxtail millet leaves, stems, roots, inflorescences and seeds at 5, 15 and 25 days after pollination were harvested and stored at −80°C after frozen in liquid nitrogen.
Seeds of Arabidopsis thaliana (Col-0), after stratification, were plated on ½MS medium with 2% sucrose and 0.7% agar for three weeks at 21–22°C with a 16 h/8 h (day/night) photoperiod and 60-70% relative humidity. Then, the young seedlings were planted on fertilized soil and grown in the same conditions.
For SiLEA14 expression profile in response to ABA, NaCl and PEG, two-week-old foxtail millet seedlings were carefully removed from soil and washed. The cleaned plants were fixed in plastic foam, and grown hydroponically in water for 1 d. Then, the seedling roots were immersed separately in water containing 100 μM ABA, 250 mM NaCl and 20% (m/v) PEG 6000 for the indicated time in Figure 3, respectively. Six seedlings were used in each treatment. After drying on the filter paper, the seedlings were harvested, and then immediately frozen in liquid nitrogen and stored at −80°C until use.
For GUS expression in response to ABA, NaCl and PEG, about 20 one-week-old proSiLEA14::GUS transgenic Arabidopsis plants were carefully removed from the plates and immersed in water containing 100 μM ABA, 250 mM NaCl and 20% (m/v) PEG 6000 for 0, 3, 6 and 18 h, respectively. For histochemical GUS staining, three-week-old proSiLEA14::GUS transgenic Arabidopsis plants were used.
Cloning and sequence analysis of SiLEA14
The full-length SiLEA14 was amplified by RACE in accordance with the manufacturer’s protocol (GeneRacer™ Kit, Invitrogen, Carlsbad, CA, USA). The products were cloned into the pMD19-T vector (Takara, Shiga, Japan) and sequenced. Primer sets used are listed in Additional file 4. The isoelectric point and molecular mass predictions were estimated using the compute pI/Mw tool (http://expasy.org/tools/pi_tool.html). Analysis of protein hydropathy was done by constructing hydropathy plots with the Kyte and Doolittle algorithm (http://ipsort.hgc.jp/) . Motif analysis was performed using the Pfam program (http://www.ebi.ac.uk/Tools/InterProScan/). The grand average of hydropathy (GRAVY) and instability index of deduced proteins were predicted using the ProtParam program (http://au.expasy.org/tools/protparam.html). Sequence similarities were determined using the BLAST program and the GenBank database on the NCBI web server. The complete amino acid sequences of subgroup 5C LEA proteins were used to construct a phylogenetic tree. Sequence alignment was performed with ClustalW and adjusted manually. A phylogenetic tree was constructed with the neighbor-joining method using the MEGA4.0 program . Sequence logos for subgroup 5C LEA14 were obtained with the WebLogo website http://weblogo.berkeley.edu/logo.cgi .
RNA extraction, semi-quantitative PCR and qRT-PCR
Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and first-strand cDNA was prepared with SuperScript III Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) after digestion with RNase-free DNase I (Takara, Shiga, Japan). The semi-quantitative RT-PCR were conducted as follows: 95°C for 3 min, then 25°Cycles of 95°C for 30 s, 54°C for 30 s, and 72°C for 30 s for both SiLEA14 and Actin2. For qRT-PCR, 100 ng of cDNAs were used as template in a 20 μL reaction system, containing 10 μL 2× SYBR Premix Ex Taq II (TaKaRa, Shiga, Japan), and 0.5 μM each specific forward and reverse primer (Additional file 4). Amplification was performed using the Bio-Rad CFX96 Real-Time PCR System C1000 Thermal Cycler (Bio-Rad, USA) as follows: 95°C for 30 s, 35°Cycles of 95°C for 10 s, 60°C for 10 s, and 72°C for 10 s. Arabidopsis Actin2 (accession number: NM_180280) and foxtail millet actin7 (accession number: NM_001280818) were used as the endogenous references. Primers used were listed in Additional file 4.
Subcellular localization of SiLEA14
The coding sequence of SiLEA14 without the terminating codon was amplified and inserted into the XbaI/SmaI sites of pROK219-GFP to generate the construct pROK219-SiLEA14-GFP. Onion epidermal cells were bombarded with the constructs pROK219-GFP and pROK219-SiLEA14-GFP, which were validated by sequencing, using a particle gun-mediated system PDS-1000/He (Bio-Rad, Hercules, CA, USA). Foxtail millet protoplast isolation and transfection were carried out according to the procedure described by Zhai et al. . Root tissues from 7-day-old seedlings were sliced and then incubated in a solution containing 1.5% Cellulase RS, 0.75% Macerozyme R10, 0.6 M mannitol, 10 mM MES, 0.1% BSA and 1 mM CaCl2 for 4–5 h at 28°C in the dark with gentle swirling (50 rpm). The constructs pROK219-SiLEA14-GFP and pROK219-GFP were incubated with protoplasts and 40% PEG 4000 for 20 min at room temperature for transient transformation, respectively. GFP signals were observed with a confocal laser scanning microscopy (LSM 510, Carl Zeiss MicroImaging GmbH, Jena, Germany).
Assay for salt-stress tolerance of E. colitransformants
The coding sequence of SiLEA14 without the stop codon was amplified and cloned into the EcoRV/XhoI sites of pET30a(+) to construct the expression vector pET30a-SiLEA14, which was then transformed into E. coli host strain BL21. The pET30a(+) empty vector was used as the control. The expression of SiLEA14 in the recombinant cells was confirmed by SDS-PAGE analysis (Additional file 6). Transformed E. coli BL21 cells carrying pET30a-SiLEA14 or pET-30a (+) were grown in LB liquid medium supplemented with 100 μg/ml ampicillin overnight at 37°C, respectively. The bacterial cultures were diluted 100-fold using fresh liquid LB, and allowed to incubate for 2–3 h at 37°C until OD600 = 0.5–0.6. Isopropylthio-β-D-galactoside was then added to the cultures to a final concentration of 1 mM, and the bacteria were cultured for a further 4 h at 30°C to induce expression of the inserted gene. All induced cultures were adjusted to OD600 = 0.6 using fresh liquid LB medium with 100 μg/ml ampicillin. To measure responses to salt stress, the samples were diluted by 200-, 500-, 1000-, 2000- and 4000-fold with fresh LB medium supplemented with 100 μg/ml ampicillin. Five microliters of each diluted sample were plated on LB agar plates, LB agar plates supplemented with 600 mM KCl and 600 mM NaCl, respectively. After incubation for 12 h on LB agar plates or 24 h on LB agar plates supplemented with 600 mM KCl and 600 mM NaCl at 37°C respectively, the numbers of colonies were calculated. Growth was measured at least three times.
Generation of transgenic plants
For SiLEA14 overexpression in Arabidopsis, the coding region of SiLEA14 was amplified and ligated into the HindIII/SpeI sites of pSB1300, which was kindly provided by Prof. Shuhua Yang of the College of Biological Sciences, China Agricultural University, China, to generate the construct pSB1300-SiLEA14 in which SiLEA14 was controlled by Super promoter. For SiLEA14 promoter assay, the putative SiLEA14 promoter was isolated from the foxtail millet genome using PCR and cloned into the SalI/EcoRI sites of pCAMBIA1391-GUS to generate the construct pCAMBIA1391-proSiLEA14-GUS. After validation by sequencing, the constructs were introduced into Agrobacterium tumefaciens strain GV1301, and transformed into Arabidopsis by the floral dip method . Seeds were obtained following self-pollination.
For SiLEA14 overexpression in foxtail millet, the coding region of SiLEA14 fused with a flag tag at the 3′end was amplified and ligated into the SacI/KpnI sites of the binary vector pCOU , which includes the ubiquitin promoter to drive transgene expression. After confirmation by sequencing, the recombinant plasmid pCOU-SiLEA14-flag was introduced into A. tumefaciens strain LBA4404. Transgenic foxtail millet plants were obtained by Agrobacterium-mediated transformation as described previously ,. Seeds were obtained following self-pollination.
Abiotic stress-tolerance assay of SiLEA14 transgenic Arabidopsis
Seeds of WT and T3 transgenic Arabidopsis plants were surface sterilized by the vapor-phase method , and sown on MS, MS + 125 mM NaCl and MS + 250 mM mannitol media, respectively. After 3 days vernalization at 4°C, they were cultured in a controlled chamber (22°C, humidity 40-50%, 120–150 μmol/m−2 s−1 under 16-h light/8-h darkness). Photographs were taken after 7 days culture. Fresh and dry weights of each sample were calculated based on the average weight of 20 individual plants.
Abiotic stress tolerance assay of SiLEA14 transgenic foxtail millet
For analysis in the germination stage, 30–40 seeds of both WT and T2 transgenic millet were geminated on the filter paper in a Petri dish wet with water (as control) or water containing 150 mM NaCl, 250 mM NaCl, 10% PEG, 20% PEG and10 μM ABA for 1 day at 30°C, respectively. Photographs were taken after 4 and 9 days, respectively. Shoot and root lengths were measured.
For salt tolerance assay in soil, two-week-old seedlings were irrigated with water, 150 and 250 mM NaCl solution every 3 days, respectively. After 6 days, the phenotypes of the transgenic lines and WT were investigated. Six to eight plants grown in one plot were used in each experiment. Two fully expanded young leaves from each foxtail millet plant per plot were harvested and cut into 1 cm segment for electrolyte leakage measurement.
For drought tolerance assay in soil, two-week-old seedlings were deprived of water for 7 days. Subsequently, the plants were irrigated with water and grown for 3 days. Six to ten plants grown in one plot were used in each experiment. The survived plants were counted. Above-ground parts of treated seedlings were collected and used to measure proline and soluble sugar contents.
Electrolyte leakage assay
Leaf tissue (0.1 g) from each sample was washed and immersed in 20 mL deionized water with 150 rpm shaking for 16 h. The initial electrical conductivity (L1) of the sample was detected using a FE-30 conductivity meter (Mettler-Toledo, Columbus, OH, USA). Then, the samples were autoclaved at 121°C for 10 min and cooled to room temperature. The ultimate conductivity (L2, maximum conductivity of tissues) was measured. Relative electrical conductivity (L) was calculated as the ratio of L1/L2.
Proline content measurement
Free proline content of foxtail millet plants was measured using the method described by Bates et al. . Leaf tissue (0.1 g, dry weight) was used to extract free proline in 3% sulphosalicylic acid at 95°C for 15 min. Then, 2 mL of supernatant was transferred to a new tube and reacted with 2 mL acetic acid and 2 mL acidified ninhydrin reagent for 30 min at 95°C. Next, 5 mL of toluene was added to the tube with full shaking. The absorbance of the toluene layer was determined at 520 nm.
Soluble sugar content measurement in foxtail millet leaves
Soluble sugar content of foxtail millet plants was examined using the method of Yemm and Willis . Leaf tissue (0.1 g, dry weight) was used to extract soluble sugar in 7 mL of 80% ethanol with constant stirring at 80°C for 2 h. Ethanol was evaporated in the boiled water bath. Then, 1 mL water and 5 mL of 0.15% anthrone solution was added. After incubation at 95°C for 15 min and cooling to room temperature, the absorbance of the reaction solution was determined at 620 nm. Glucose was used as a standard.
Histochemical GUS staining
Histochemical GUS staining was performed as described by Jefferson et al. . After the GUS staining, Arabidopsis seedlings were treated with 70% ethanol to remove chlorophyll from the GUS-stained tissue.
The survival rate, fresh/dry weights, relative electrolyte leakage rate, proline content and soluble sugar content data were subjected to Student’s t-test analyses using GraphPad Prism 5. All of the experiments were repeated three times.
Availability of supporting data
The SiLEA14 sequence was deposited in GenBank with an accession number of KJ767551. The data supporting the results of this article are included within the article and its additional files.
MW, PL and JY conceived and designed the experiments. MW, PL, CL, YP and XJ performed the experiments. QZ and DZ participated in the coordination of the experiments. MW and JY wrote the original manuscript. JY thoroughly revised the manuscript and finalized the manuscript. All authors read and approved the final manuscript.
This work was supported by the National Basic Research Program of China (Grant No. 2012CB215301 to YJ).
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