Figure 6

Assay for NaCl, PEG and ABA stress on SiLEA14 overexpression foxtail millet germination. (A) Diagram of the T-DNA region of the binary vector pCOU-SiLEA14-flag. LB, left border; RB, right border; 35S-Pro, cauliflower mosaic virus 35S promoter; Hpt, hygromycin B phosphotransferase gene; Ubi-pro, maize Ubiquitin promoter; SiLEA14-flag, SiLEA14 gene fused with a flag tag; NOS, nopaline synthase terminator. (B) QRT-PCR analysis of SiLEA14 in transgenic foxtail millet lines (L68, L76 and L78). Actin7 was used as an internal control. (C-D) The phenotype of transgenic and WT foxtail millet under various abiotic stress treatment during the germination stage. The T₂ seeds soaked in water (as control) or in water containing 150 mM NaCl, 250 mM NaCl, 10% PEG, 20% PEG and 10 μM ABA for 1 day at 30°C and then placed on the filter paper in a Petri dish wet with the same solutions mentioned above for 4 and 9 days, respectively. This experiment had three replicates, and each experiment comprised 30–40 plants. Bar = 1 cm. (E-F) The shoot length of transgenic and WT foxtail millet germinated under above conditions for 4 and 9 days, respectively. (G-H) The root length of transgenic and WT foxtail millet germinated under above conditions for 4 and 9 days, respectively. Data in B, E-H represent means and standard errors for three biological replicates. Statistical significance was determined by Student’s t-test. *P < 0.05; **P < 0.01.