Expression of GUS gene in pro SiLEA14 ::GUS transgenic Arabidopsis upon various stresses determined by qRT-PCR. One-week-old seedlings were treated with 100 μM ABA, 250 mM NaCl and 20% (m/v) PEG 6000 solution for 0, 3, 6 and 18 h, respectively. Total RNA was extracted from at least 30 whole seedlings per treatment. The expression of GUS in proSiLEA14::GUS transgenic Arabidopsis was determined by qRT-PCR using GUS-specific primers (Additional file 4). Actin2 was used to normalize the gene expression. Data represent means and standard errors for three biological replicates.