Figure 3

SiLEA14 expression patterns in foxtail millet determined by qRT-PCR. (A) Tissue-specific expression of SiLEA14. Total RNA was extracted from roots (R), stems (S), leaves (L), inflorescences (I) and seeds at 5 (S1), 15 (S2) and 25 (S3) days after pollination, respectively. Foxtail millet Actin7 was used as an internal control. (B-D) SiLEA14 transcripts accumulation in response to various treatments. Two-week-old foxtail millet seedlings were treated with 100 μM ABA (B), 20% (v/v) PEG 6000 (C) and 250 mM NaCl (D) for the indicated times, respectively. Total RNA was extracted from the above-ground parts of ten plants at the indicated times after the treatments. Data represent means and standard errors for three biological replicates.