- Research article
- Open Access
Domestication-driven Gossypium profilin 1 (GhPRF1) gene transduces early flowering phenotype in tobacco by spatial alteration of apical/floral-meristem related gene expression
BMC Plant Biologyvolume 16, Article number: 112 (2016)
Plant profilin genes encode core cell-wall structural proteins and are evidenced for their up-regulation under cotton domestication. Notwithstanding striking discoveries in the genetics of cell-wall organization in plants, little is explicit about the manner in which profilin-mediated molecular interplay and corresponding networks are altered, especially during cellular signalling of apical meristem determinacy and flower development.
Here we show that the ectopic expression of GhPRF1 gene in tobacco resulted in the hyperactivation of apical meristem and early flowering phenotype with increased flower number in comparison to the control plants. Spatial expression alteration in CLV1, a key meristem-determinacy gene, is induced by the GhPRF1 overexpression in a WUS-dependent manner and mediates cell signalling to promote flowering. But no such expression alterations are recorded in the GhPRF1-RNAi lines. The GhPRF1 transduces key positive flowering regulator AP1 gene via coordinated expression of FT4, SOC1, FLC1 and FT1 genes involved in the apical-to-floral meristem signalling cascade which is consistent with our in silico profilin interaction data. Remarkably, these positive and negative flowering regulators are spatially controlled by the Actin-Related Protein (ARP) genes, specifically ARP4 and ARP6 in proximate association with profilins. This study provides a novel and systematic link between GhPRF1 gene expression and the flower primordium initiation via up-regulation of the ARP genes, and an insight into the functional characterization of GhPRF1 gene acting upstream to the flowering mechanism. Also, the transgenic plants expressing GhPRF1 gene show an increase in the plant height, internode length, leaf size and plant vigor.
Overexpression of GhPRF1 gene induced early and increased flowering in tobacco with enhanced plant vigor. During apical meristem determinacy and flower development, the GhPRF1 gene directly influences key flowering regulators through ARP-genes, indicating for its role upstream in the apical-to-floral meristem signalling cascade.
Modern crop species are the incredible outcome of the selection force applied on wild plant species by millennia of human-mediated selection, termed as plant domestication. Such evolutionary processes entail events and phenomenon at morphological and genetic levels those have led to certain morpho-transitions in the crop species. Such traits consist of a reduction in grain shattering and seed dormancy in cereals [1, 2]; increased apical dominance in maize ; increase in seed and pod size, day- neutral flowering in pulses [4, 5]; increased fiber length and quality in cotton [6–8]; increased fruit size in tomato ; shorter stolons and larger tubers of potato  and many others. Comparative genomics of such acquired phenotypes had increased our understanding of the key genes and trans-factors underlying morphological variations in the antecedents and their descendants. For example, Ghd7 transcription factors in rice for grain number, plant height, flowering time ; tb1 transcriptional regulator and ZmCCT gene in maize for plant architecture [12, 13]; Vrs1 gene in barley for inflorescence architecture ; Sh1 transcriptional regulator in Sorghum for non-shattering traits  and HaFT1 transcriptional regulator in Sunflower for improved flowering time . Such information has provided obvious clues for the evolutionary signatures of such morphological characters evolved under crop domestication.
To understand the genetic basis of plant domestication, system-wide comparative gene expression analyses have been performed at different developmental stages of cotton fiber cells harvested from wild and domesticated forms of modern allopolyploid species (Gossypium hirsutum L.) . A large number of genes showed differential up- or down-regulation during fiber development. Interestingly, prolonged fiber growth in the domesticated cotton was associated with enhanced hormone signalling genes, delayed stress-responsive gene expression and predominantly modulation of cell-wall structural genes . The latter has drawn much scientific attention to their role in the cell-wall organization, cellular growth and plant development . The dynamic rearrangement of actin filaments is a prerequisite for proper cell wall development because different cell wall proteins work in concordance to maintain stability between filamentous and monomeric actin. It is evident that cell-wall structural protein, especially members of the profilin gene family show up-regulation of at least 400 folds in the domesticated diploid and allotetraploid cotton species than their respective ancestral wild counterpart.
Profilin genes belong to a multigene family extensively diversified across plant species [20–23]. There are five profilin genes PRF1, PRF2, PRF3, PRF4, and PRF5 present in Arabidopsis [20, 24, 25]; three members in tobacco ; five members in maize [21, 23]; five members in parsley ; six members in cotton . Plant profilin is a small cytosolic protein composed of 129–133 amino acids with a low molecular mass of 12-15 kDa that binds to actin monomer in 1:1 complex . Various studies have revealed conserved functions of profilins ranging from lower to higher eukaryotes. In yeast, the profilin genes are involved in cell wall maintenance via actin sequestering, nucleation and cytokinesis [28, 29].
In response to the endogenous or external signals, the cytosolic actin protein undergoes profilin-mediated polymerization and/or depolymerization in a synchronized manner resulting in the cytoskeleton modulation. Profilin promotes the actin-filament formation upon polymerization of sequestered actin monomers present in the cell . In Drosophila, profilins are necessary for actin polymerization and its localization throughout stages of development [31, 32]. Profilin genes have also been characterized for their essential roles in plants, such as in pollen formation in maize  and tomato ; in root nodule development of bean ; in cell elongation, cell shape maintenance, and flowering of Arabidopsis [24, 35] and in initiation and elongation of cotton fiber . At the molecular level, profilin proteins contribute to the assembly and activity of macromolecular complexes such as polyphosphoinositides [36, 37], Arp2/3 complex , annexin , prolin-rich ligands  and regulating the cell signalling in vivo . So, it determines the key morphological and anatomical traits during plant growth and development.
Several genes are involved in floral induction and flower development. This extraordinarily complex mechanism of flowering is controlled by a number of parallel and/or overlapped pathways governed by diverse genetic networks. Floral meristem identity genes such as AP1 , AP2 , LFY ; floral pathway integrators such as SOC1 [45, 46], FT1  and FLC1 gene  strongly influence the flowering mechanism in plants. The FT m-RNA/proteins are synthesized in leaves and get transferred to shoot apex that in result induces expression of downstream flowering genes at apex mainly through binding with FD transcription factors . Eventually, it converts the apical shoot meristem into flowering meristem and induces flowering [49, 50]. The FT gene is repressed by EARLY FLOWERING6 (ELF6) gene and delays the flowering process in Arabidopsis . In general, the floral transition is repressed by flowering locus C (FLC) gene that negatively regulates the genes involved in floral pathway integrators . The RELATIVE OF EARLY FLOWERING 6 (REF6) suppresses the expression of FLC gene and promotes flowering .
However, information is scarce about the involvement of profilin in the flowering mechanism. Profilin is a multifunctional protein and its overexpression in plants results into longer roots and root hair, expanded leaf surface area, accelerating the commencement of flowering in Arabidopsis [35, 54, 55], elongated cells in transgenic tobacco culture  and early progression of developmental phase in the cotton fiber . Whereas under-expression of profilin gene exhibits smaller phenotype, with at least 40 % reduction in the number of leaves. Reduced expression levels of profilin in Arabidopsis delayed initial germination rate and development of seedlings . Defects in rosette leaf morphology and inflorescence stature were reported in response to the lack of PRF1/PRF2 gene expression in Arabidopsis . However, details of the profilin interaction with other genes and trans-factors during flowering are to be explored hitherto.
Plant profilins have their conventional role in actin polymerization and depolymerization in vivo. Notwithstanding striking discoveries of the genetics of the cell-wall organization in plants , limited information is available on the molecular function of profilin and its corresponding network during cell-signalling in the developing apical/floral-meristem. In the present study, we aim to investigate the role of profilin and its molecular interaction considered important in the apical meristem identity and differentiation. This is possible through the dynamic expression interactions among key positive and negative regulators of flowering time phenotypes. Our approach was to use transgenic stocks for up- and down-expression of profilin gene in tobacco; and functional characterization of profilin during flower induction and development. Expression dynamicity of key genes/factors was noted, illuminating possible changes induced in the developmental programs in response to altered profilin levels of the cell. This approach is very useful as a preamble to identify candidate genes interacting with profilin structural protein determining the apical meristem architecture, and governing several other important phenotypic traits during plant development.
Constitutive overexpression of cotton profilin 1 (GhPRF1) in tobacco shows early flowering
Previously, we had explored the evolution of global gene expression patterns under cotton domestication through comparative transcript profiling experiments performed on the fiber cells harvested at three developmental stages from wild and domesticated accessions of allopolyploid Gossypium barbadense and G. hirsutum, using a microarray platform that interrogates 42,429 unigenes [17, 18]. Notably, cell-wall related profilin gene family is one of the structural gene families that has been highly up-regulated parallelly and independently in the domesticated accessions of both allopolyploid species in contrast to their respective wild counterparts. To study the role of cotton profilin structural genes in other plant phenotypes, the spatial expression analysis was performed in ectopically expressed profilin transgenic tobacco lines. The full-length GhPRF1 gene (accession number EF143832) consisting of 402 bp was constitutively expressed under the control of Cauliflower Mosaic Virus 35S (CaMV35S) promoter with double enhancer region (35Sde) in tobacco (Nicotiana tabacum cv. Xanthi), along with nos:nptII:pA gene cassette as a plant selection marker (Fig. 1a). The 35Sde comprises of a repeat of the −90 to −343 region of the 35S promoter upstream of the wild- type 35S promoter that functions as an enhancer . Along with two more binary constructs having GhPRF1 gene and gus-gene were developed for the generation of gus-reporter lines and RNAi lines, respectively having nptII gene as plant selection marker (Fig. 1b, 1c). Using leaf explants, Agrobacterium-mediated genetic transformation of tobacco was performed and twenty seven independent transgenic lines with 35Sde-GhPRF1 gene were developed. These independent lines were screened for the transgene integration through PCR using nptII specific primers. The PCR positive transgenic lines along with in vitro regenerated control Xanthi plants were simultaneously transferred to the controlled growth conditions.
In semi-quantitative expression screening of PCR positive lines, significant over-expression of GhPRF1 gene were recorded in several transgenic lines including two lines Pf-Ox4 and Pf-Ox17 that showed a significant increase in the profilin transcript level compared to the control plants (Fig. 2a, 2b). Transgenic lines Pf-Ox4 and Pf-Ox17 showed more than 20 % increase in the GhPRF-transcript level in the leaf tissues and up to 17 % up-regulation in the reproductive organs (Fig. 2c, 2d). The two high GhPRF1-transgene expression lines exhibited developmental phenotypes assessed at different stages of plant development (Fig. 2e). Such qualitative phenotypic changes were similar in both the transgenic lines.
Ectopic expression of GhPRF1 leads to the hyperactivation of apical meristem and alters spatial expression of meristem-related CLAVATA1 gene
The complimentary DNA of GhPRF1 was expressed under the control of CaMV35S promoter in Xanthi. After transgene-based screening of putatively transformed lines, one of the most significant changes examined in both transgenic lines Pf-Ox4 and Pf-Ox17 was the hyperactivation of apical meristem after at least 50 days of vegetative growth post-transplantation (dpt) in the soil and its further differentiation into floral meristem at 99-100 dpt than control plants at 110-112 dpt (Fig. 2f, 2g). To ascertain if the enhanced apical growth and its early conversion into floral meristem were due to faster apical-meristematic cellular division and differentiation, elongation of internodal regions and conversion rate of apical-to-floral meristem were determined for representative Pf-Ox4 and Pf-Ox17 overexpression lines. The overexpression lines resulted in the significant increase in the organ (leaf) differentiation and expansion than control lines (Fig. 2h, 2i). The apical shoot meristem was converted into floral meristem earlier in Pf-Ox4 and Pf-Ox17 overexpression lines than control plants resulting into increased number of fully developed flowers. These differences in the developmental conversion of apical meristem can be attributed, at least in part, to the overexpression of trans-GhPRF1.
Further, to identify temporal alterations in the regulation of a complex genetic-network of apical meristem activity in GhPRF1 overexpression lines, leaf tissues were harvested at the similar developmental time points avoiding physiological variations between transgenic and control lines. Similarly, floral bud tissues were harvested from both transgenic and control lines. Comparative expression analyses of selected genes CLAVATA1 (CLV1) and WUS were performed in both Pf-Ox4 and Pf-Ox17 transgenic lines along with control plant. These genes/trans-factors have been reported for their direct role in the regulation of meristem identity, maintaining a balance between cell proliferation and organ formation at shoot/flower meristems (Table 1).
In semi-quantitative expression study, the endogenous transcript level of CLV1 receptor-kinase gene varied enormously across developmental stages. CLV1 gene encodes a putative receptor-like kinase which plays an important role in signal transduction during shoot induction. Expression of CLV1 results into the formation of shoot primordia and increases the proliferation of undifferentiated mass of cells destined for shoot formation. Therefore, it was decided to examine the expression levels of CLV1 receptor-kinase gene in both vegetative and floral tissues. Reverse transcription-PCR was performed to determine if constitutive overexpression of CLV1 receptor-kinase gene transcript is detectable in both Pf-Ox4 and Pf-Ox17 transgenic lines. Interestingly, the expression of CLV1 receptor-kinase gene showed continuous spatial alteration in its expression levels across tissues and developmental stages of both Pf-Ox4 and Pf-Ox17 overexpression lines (Fig. 3a, 3b). Up to 14 % increase in the expression level of CLV1 receptor-kinase gene in the vegetative tissue of Pf-Ox4 line was observed in comparison to control lines. On the contrary, more than 30 % reduction in the expression level of CLV1 receptor-kinase gene was observed in the floral tissues of Pf-Ox4 and Pf-Ox17 lines than vegetative tissues (Fig. 3c, 3d). Based on these findings, profilin-mediated functional polymerization of proteins combined with CLV1 receptor-kinase is adequate for the activation and determinacy of apical meristem that accomplishes the apical-to-floral meristem conversion. Further, to explore if CLV1 receptor-kinase is a functional contributor to such an extraordinarily complex process of meristem growth, development and differentiation, in vitro organogenesis experiments were performed in tobacco explants cultured on MS medium  supplemented with auxin (NAA; 0.1 mg/l) and cytokinin (BAP; 1.0 mg/l) (Fig. 4a). Previously, in our laboratory the effect of micronutrient Boron (B) has been reported on the magnitude of organogenesis in tobacco when cultured on minimal (<0.1 mM), optimal (0.1 mM) and maximal (1.0 mM) B-concentrations . It was evident that explants expansion and growth was more in minimal B-concentration than other concentrations, however, in vitro shoot induction was the highest at an optimal concentration of B-supplementation (Fig. 4b, 4c). To determine if CLV1 gene has a certain role in the initiation and progression of shoot development (during organogenesis), the temporal CLV1 expression was analysed under different B-concentrations in 7 days and 15 days old cultured explants.
Interestingly, no significant change in the basal expression level of CLV1 gene across B-treatment was observed at 7 days old cultured explants, however, significant alterations were observed at 15 days old tissues (Fig. 4d, 4e). CLV1 expression was substantially increased in the shoot-buds cultured on optimal B-supplementation than minimal or maximal concentrations. This suggests that CLV1 expression in shoot-buds is proportionate with the magnitude of organogenesis (shoot induction/formation). This may be assumed that in 7 days old cultures, initially the explants undergo callogenesis followed by organogenesis, after 15 days of the culture period (Fig. 4b). At this stage of development, the callus-foci are converted into shoot primordia and important genes/factors such as CLV1 gene considered to be accountable for organogenesis are most likely to be expressed at this shoot-bud stage.
GhPRF1 overexpression shows enhanced organogenesis in vitro
Given that the CLV1 expression was radically increased in the emerging shoot buds in vitro, what would be the magnitude of organogenesis in the shoot buds transformed with GhPRF1 gene intended to enhance CLV1 expression? Since in vitro genetic transformation experiments were carried out on kanamycin (100 mg/l) antibiotic selection marker that may negatively influence organogenesis, genetic transformation experiments with 35S-gus construct were performed parallelly, therefore avoiding detrimental effects of kanamycin on the magnitude of shoot induction in 35Sde-GhPRF1 transformed explants (Fig. 1b). This was bolstered in the genetic transformation experiments using GhPRF1 transgene where shoot formation was observed up to 37 % in 35Sde-GhPRF1, 23 % in 35S-gus transformed explants and 29 % in optimal B-supplemented media, of the inoculated leaf explants (Fig. 5). This observation suggested that GhPRF1 overexpression directed the up-regulation of CLV1 expression during apical meristem initiation.
RNAi of GhPRF1 showed delay in flowering and reduced flower number than overexpression lines
To determine, if early flowering time phenotype as shown by Pf-Ox4 and Pf-Ox17 transgenic lines was attributed to profilin overexpression, down-expression of GhPRF1 gene through RNAi was experimented. For this purpose, a GhPRF1-RNAi gene construct was developed using pHANNIBAL cloning vector  having an intron of 742 bp flanked by inverted and palindromic sequences of full-length profilin gene (Fig. 1c). Using GhPRF1-RNAi construct, Agrobacterium-mediated genetic transformation of tobacco leaf explants was performed. Twenty-seven transgenic lines were developed and screened for transgene presence with the help of PCR. All PCR positive lines were screened for % GhPRF1 silencing using RT-PCR; and six independent transgenic lines were identified with substantial down-regulation of profilin expression. In particular, line Pf-Si23 showed maximum 36 % down-expression of GhPRF1 transcript level compared to Pf-Ox4 line. At phenotypic level, the plant height of down-expression Pf-Si23 line was significantly lower than Pf-Ox4 line but similar to the control plants (Fig. 6a). The average number of flowers per Pf-Si23 plant was lesser than both Pf-Ox4 line and control plants (Fig. 6b, 6c, 6d, 6e). The flowering initiation time of transgenic line Pf-Si23 was recorded at least 10 days later than Pf-Ox4 line and similar to the control plants (Fig. 6f). Also, delayed conversion of apical shoot meristem to floral meristem and with reduced number of flowers per plant in Pf-Si23 line proved that profilin expression controls apical meristem development, differentiation, floral initiation and development through its interaction with other known genetic factors.
Overexpression of GhPRF1 regulates floral determinacy by arrested expression of WUSCHEL trans-factor
Prompted by an intriguing observation made on the increased expression of CLV1 receptor-kinase gene in response to overexpression of GhPRF1 in transgenic Pf-Ox4 and Pf-Ox17 lines achieving hyperactivation of apical meristem, it was relevant to determine if the expression patterns of other coordinating factor(s) involved in the genetic regulation of floral determinacy show any dynamicity in their expression patterns. As summarized in Table 1, WUSCHEL (WUS) homeodomain transcription factor is involved in stem cell activity in the central zone of apical meristem and controls floral determinacy. WUS controls the stem cell fate and maintain its expression level by up-regulating the CLV gene through feedback regulation . Therefore, the expression analysis of WUS gene in both vegetative and floral tissues of both Pf-Ox4 and Pf-Ox17 lines was performed. It was observed that both GhPRF1 overexpressed lines showed down-regulation of WUS transcripts level in the floral buds than the vegetative tissues. This may be expected that high accumulation of profilin in Pf-Ox4 and Pf-Ox17 lines arrests WUS expression either directly or through coordinated factors, thus adversely influencing the feedback loop during floral determinacy .
Further, to determine if WUS expression varies in comparison to the magnitude of organogenesis and in coordination with CLV1 gene expression, the temporal WUS expression was analysed in 7 days and 15 days old leaf explants cultured under different B-concentrations. Surprisingly, in maximum B-supplemented callus tissues, at least 3 fold increased expression of WUS gene in 7 days old cultures was observed than 15 days old cultures (Fig. 4e). At this stage of culture, the CLV1 expression is examined at basal level (Fig. 4d) and is congruent with established correlation of the two genes under consideration. On the contrary, in 15 days old cultures (shoot-buds) WUS expression level was enhanced up to 1.5 fold in optimal B-supplemented cultures than maximal B-supplemented cultures. This is in concordance with the magnitude of organogenesis reported on optimal B-supplementation in tobacco (unpublished data). At this stage of development, up-regulation of WUS leads to the overexpression of CLV1 gene thus controlling WUS at transcript level through feedback and simultaneous progression of shoot primordia at the phenotypic level. Taken together, our work shows that overexpression of profilin down-regulated WUS expression level via modulated CLV1 transcript levels during floral initiation and development.
Dynamicity of coordinated expression patterns of key flowering genes in GhPRF1 overexpression and silencing lines
Since early flowering time phenotype was observed in both Pf-Ox4 and Pf-Ox17 lines, it is to determine if key positive or negative flowering regulators show any dynamicity in their expression patterns? Coordinated profilin interaction network with key flowering control genes was predicted in silico using STRING 10 online tool . The prediction based interaction map suggested distinct protein clusters of key flowering genes and cytoskeleton- related genes (Fig. 7a). High interaction is observed within meristem-related CLV1, WUS and key flowering genes such as LFY, AP1 and cytoskeleton proteins. However, the low interaction was evident between the two protein clusters (Fig. 7a). Further to determine the dynamicity of such profilin interaction network with flowering regulators predicted in silico, the spatial expression of positive flowering regulators FT4, SOC1 and AP1 genes [42, 45–47, 49, 50] and negative regulators FLC and FT1 gene [48, 52, 67] was examined in the vegetative and the floral bud tissues of both overexpression and down-expression transgenic lines. The class ‘A’ flowering AP1 gene showed 3.5 fold increased expression in the vegetative tissue of Pf-Ox4 line than control plants or Pf-Si23 line (Fig. 7a). This AP1 gene expression showed at least 700 folds increase in 100 dpt old floral bud tissue than vegetative tissue of Pf-Ox4 line, which was similar to 110 dpt old reproductive tissue of control plant (Fig. 7a). This data highlighted the mechanistic link between profilin overexpression and early flower primordium initiation via up-regulation of AP1 gene. Similarly, FT4 gene, a positive flowering regulator which is initially synthesized in the leaves and travels to apical meristem showed more than 16 folds increase in its expression in the vegetative tissues of Pf-Ox4 line than Pf-Si23 line and control plants (Fig. 7b). Whereas in the reproductive tissues of Pf-Ox4 line and control plant, FT4 expression decreased drastically, highlighting its role in the up-regulation of flowering regulators SOC1, LFY and AP1 genes. These genes are responsible for the activation of class ‘B’ genes during floral development. In response to elevated FT4 expression in the vegetative tissue of Pf-Ox4 line, homeodomain SOC1 transcription factor showed at least 1.5 fold increase in its expression level than control plant (Fig. 7b). However, SOC1 expression in Pf-Ox4 line was similar to the Pf-Si23 line which showed that the up-regulation of SOC1 transcription was intermediate to the flowering mechanism for up-regulation of AP1 gene. Whereas no significant difference in SOC1 expression was observed in the reproductive tissues of Pf-Ox4 lines and control plant (Fig. 7b). Therefore, early flowering time phenotype is controlled by the molecular interaction of up-regulated FT4 gene with SOC1 and AP1 genes. This is further confirmed by the Pf-Si23 line which showed flowering similar to control plants despite the up-regulation of positive regulator SOC1 gene. This observation confirmed the central role of profilin in early flower primordium formation via up-regulation of AP1 gene. Conversely, negative flowering regulators FLC and FT1 genes in the vegetative tissues of overexpression line in comparison to the control plant showed at least 2.5 and 1.18 fold decrease in their respective expression levels. Whereas FT1 gene which is a negative regulator of flowering in tobacco  showed 1.5 fold reduced expression level in the reproductive tissues of Pf-Ox4 line compared to the control plant (Fig. 7c). This coordinated expression patterns of both negative and positive regulators in the vegetative tissues are contemplated to advance the cellular milieu for flower induction. Since Pf-Si23 line showed flowering time similar to control plants, the expression patterns of positive regulators AP1, SOC1 and FT4 were analysed in the floral buds of Pf-Ox4 and Pf-Ox17 lines. FT4, SOC1 and AP1 genes showed coordinated overexpression in both Pf-Ox4 and Pf-Ox17 lines, whereas key negative regulators FLC and FT1 genes exhibited significant down-regulation across tissues and stages (Fig. 7c). Thus, on the acquisition of flower induction in both Pf-Ox4 and Pf-Ox17 lines, the overall coordinated regulation of positive and negative flowering regulators in vegetative and floral buds is remarkable.
GhPRF1 acts upstream to the apical-to-floral meristem signalling cascade via coordinated expression of ARP4 and ARP6 genes
To further examine the role of profilin upstream to the coordinated spatial regulation of flowering regulators in the overexpression lines, we investigated the cellular and molecular effectors including ABP1, PIP, and ARP genes involved in the apical-to-floral meristem signalling cascade. The expression patterns of ABP1, PIP, and ARP genes (especially ARP4 and ARP6 known for their defined function in flower development; Table 2) were analysed in the vegetative and floral bud tissues of both the overexpression and down-expression transgenic lines. Remarkably, ARP4 gene showed 2.3 fold reduced expression in the vegetative tissue of Pf-Ox4 line than RNAi line and control plant (Fig. 8a). Subsequently, ARP4 gene expression showed at least 4-fold decrease in floral bud tissues of Pf-Ox4 line than the similar tissues of control line which was at least 2 fold less than the respective vegetative tissue (Fig. 8a). Consistently, ARP6 gene, another profilin-associated flowering regulator showed 2.5 fold down-expression in the vegetative tissue of Pf-Ox4 line; and 1.2 fold increased expression in Pf-Si23 RNAi line than the control plant (Fig. 8b). Whereas in reproductive tissues of the Pf-Ox4 line, more than 2 fold down-expression of ARP6 gene was observed than the control plant (Fig. 8b). Overall, ARP4 and ARP6 genes showed down-expression in their expression level exclusively in the overexpression lines. However, no such variations in the spatial gene expression pattern of ABP1 and PIP genes were observed among transgenic lines. This data showed that overexpressed profilin up-regulates flowering genes expression cascade by regulating ARP4 and ARP6 gene expression which in result influence FLC1 gene and flower induction. Further, coordinated profilin interaction network including key cytoskeletal genes such as actin, and ARPs was predicted in silico using STRING 10 online tool . The prediction map suggested significant interaction among profilin, actin, and ARPs especially with ARP4 and ARP6 with high confidence limits (>0.900) (Fig. 8c). The ARP4 and ARP6 genes are important for their function in the flowering time phenotype as also highlighted by in silico analysis. This suggests that apical-to-floral meristem signalling cascade is controlled by the interaction of up-regulated profilin gene with the coordinated regulation of ARP4 and ARP6 genes. This data provided a mechanistic link between GhPRF1 gene expression and induction of flowering genes’ expression via ARP4 and ARP6 genes and provides an insight into the functional characterization of GhPRF1 gene acting upstream to the flowering mechanism. This observation was further strengthened by the analysis of Pf-Si23 line which showed ARP4 and ARP6 expression patterns and flowering time similar to control plants.
Overexpression of GhPRF1 promotes early flowering without yield penalty
GhPRF1 was overexpressed in Xanthi to evaluate the function of this gene influencing different physiological and metabolic processes. Upon vegetative growth up to 99-100 dpt, transgenic Pf-Ox4 and Pf-Ox17 lines showed most apparent flowering time phenotypes exhibiting early flower induction compared to flowering time in the control plants after 112 dpt. (Fig. 6a). Remarkably, overexpression of GhPRF1 resulted into early flowering time phenotype in tobacco. The flowering time of transgenic lines Pf-Ox4 and Pf-Ox17 was advanced up to 10–12 days than the untransformed control plants. The overexpression of GhPRF1 gene led to an increase in the flower number per plant of Pf-Ox4 and Pf-Ox17 lines compared to control plants (Fig. 6b). Such early flowering phenotype with a significant increase in flower number per plant provided obvious clues for up-regulation of genes involved in possible flowering pathways and processes. This observation was further strengthened by the coordinated expression patterns of certain negative and positive regulators involved in the flowering stimulations. Therefore, overexpression of GhPRF1 certainly led to early flowering by the up-regulation of the coordinated expression cascade during flower induction.
Due to a significant increase in the number of flowers in Pf-Ox4 and Pf-Ox17 lines than the control plants, we further investigated whether overexpression of profilin had any adverse effect on floral morphology/anatomy or on pollen formation and pollen viability.
Flowers of the overexpression Pf-Ox4 and Pf-Ox17 lines and control plants were analyzed for their morphological features at the initiation and developmental stages. The average flower length varied little between transgenic lines and control plants measuring 53.4 mm and 52.7 mm, respectively (Fig. 9a). Exterior floral parts such as corolla and calyx exhibited minimal differences between transgenic lines and control plants measuring up to 19 mm/15 mm and 50.78 mm/51.3 mm, respectively (Fig. 9b, 9c, 9d; Additional file 1). Among interior floral parts, stamens were present in 4 + 1 orientation and showed no variation in their respective lengths (Fig. 9e). The average height of gynoecium was measured 46.35 mm in transgenic lines whereas 46.31 mm in control plants (Fig. 9f; Additional file 1). Other parameters such as ovary diameter, style length, stigma shape/colour and number of ovules were quantified but no significant variations were observed between transgenic lines and control plants (Additional file 2). Further, to analyze if overexpression Pf-Ox4 and Pf-Ox17 lines show any anatomical alterations during floral development, the magnitude of organ measurements and cross-sections were compared by microscopy. No significant changes could be observed between transgenic lines and control plants (Fig. 10). Also, no differences in the anthesis-period were recorded between transgenic lines and control plants. This data suggested that overexpression of GhPRF1 in tobacco does not influence floral organ development.
To ensure if the overexpression of GhPRF1 had not influenced the pollen physiology and viability, aniline blue test of pollen grains of Pf-Ox4 and Pf-Ox17 lines was performed . Generally, pollen grains are considered viable if they had absorbed aniline blue after incubation at room temperature. One-way ANOVA test of pollen grains of both Pf-Ox4 and Pf-Ox17 lines showed the mean value of staining results equivalent to the pollen grains of control plants (p < 0.05) (Fig. 9g, 9h). Data suggest that overexpression of profilin in tobacco does not influence the pollen development and their viability. In result, healthy fruits were observed on both Pf-Ox4 and Pf-Ox17 transgenic lines without any yield penalty. The external morphology of maturing fruit of these lines along with the number of seeds per fruit was similar to the control plant. However, the number of maturing fruits per plant was higher in Pf-Ox4 and Pf-Ox17 lines than control plants (Fig. 9i, 9j).
Overexpression of GhPRF1 promotes plant height and leaf lamina expansion
It was examined that changes in profilin expression level in transgenic plants produced increased plant height via elongation of internodal regions (Additional file 3). The plant height was recorded up to 53–54 in. in the overexpression Pf-Ox4 and Pf-Ox17 lines than an average of 38.5 in. in the control plants (Fig. 2e). An average number of leaves in both transgenic lines and control plants were similar counting 34 and 32, respectively. This data indicate that the significant increase in the trait of plant height among transgenic plants was due to increased internode length than the number of nodes produced per plant. It was noted that elongation of internodal regions of both the transgenic Pf-Ox4 and Pf-Ox17 lines was not consistent across nodes, and the elongation was recorded utmost between 13th and 14th nodes compared to other nodal regions (Additional file 3). The leaf lamina of the two transgenic lines was more expanded in its length and width than to the control plants, but without any changes in the leaf thickness (Additional file 4). Comparative anatomical studies were performed to examine if the expanded leaf lamina of the overexpression Pf-Ox4 and Pf-Ox17 lines was a result of enhanced cell division, or cellular expansion during leaf growth. Transverse sections of leaves were prepared from transgenic Pf-Ox4 and Pf-Ox17 lines and control plants. But no radical change in the cell number was recorded in the epidermal layer or parenchymatous tissues. This indicates that expanded leaf lamina is not a result of enhanced cell division but the increased cellular aspect ratio during leaf development. The latter was confirmed by assessing the trichome density on the abaxial surface of the transgenic leaf and control leaf tissues. In typical microscopic view of leaf margin and the midrib regions, significant decrease in the trichome density was observed on the abaxial surface of the transgenic leaf tissues than the control plants (Additional file 5). Since trichome is an extension of epidermal layer, decrease in the trichome density suggested for epidermal cellular expansion rather than increased cell division during leaf development. Increase in plant height and expansion of leaf lamina in Pf-Ox4 and Pf-Ox17 lines could be the result of profilin-mediated actin polymerization of cell wall that may have led to the cellular expansion.
Expression evolution of profilin genes under cotton domestication
Genes and trans-factor evolved in response to the changing environmental conditions and stress factors influence selective phenotypes and most often leads to plant speciation . In crop plants, the applied selection pressure has been primarily through human-mediated artificial selection (=domestication) underlying morphological transitions in the wild antecedents of modern cultivars. These includes characters such as crop yield, fruit size, reduced seed dormancy, perennial to annual habit, enhanced apical dominance, photoperiodism, and long spinnable natural fiber [2, 6–9, 12].
The domesticated diploid and allotetraploid species of the genus Gossypium, have acquired an economically important trait of having long, spinnable fiber that had been evolved under domestication from the wild short fuzz. So, the modern crop having longer fiber is a cumulative outcome of recurrent selection during domestication and recent breeding exercises. Comparative study of such evolutionarily important characters with a morphologically variable ancestor and descendants provided a deep insight into basic principles of selection [1, 12, 14]. Previously, we explored the domestication driven temporal gene expression changes in the elongating fiber cells of the wild and domesticated allotetraploid cotton species Gossypium barbadense (AD2) . Comparative expression profiling of fiber cells at three developmental stages was performed using a microarray platform which interrogates more than forty- two thousand unigenes. Global gene expression analysis revealed the dynamicity of extraordinarily complex transcriptome of single tetraploid elongating fiber cells. Several differentially expressed gene families constituting various biological functions have been examined at different developmental time-points and between accessions. Three major class of genes have been identified as i) hormone-signalling genes ii) antioxidant genes, and iii) cell-wall structural genes .
The latter has drawn more attention where RNAseq data revealed differential overexpression of important cell wall structural protein family of profilin genes up to 400 folds in diploid and tetraploid domesticated forms than their wild ancestors . If so, does the up-regulation of profilin gene family in multiple species reflect directional selection or a co-ordinated stimulus by other important factors? In this direction, present study emphasizes the novel functions of profilins controlling flowering and plant development beyond their traditional role in cell-wall organization. In the current study, profilin1 (PRF1) gene of the genus Gossypium was characterized for its function considering the genetic diversity of this gene family among cotton homoeologs (present in co-resident A- and D-genomes in allotetraploids) and other homologous sequences (Additional files 6, 7). The distribution of exons and introns in profilin genes across species (Additional file 8), and their homologous sequence comparisons (Additional file 9) highlighted for their conserved genetic design among plant taxa .
CLV1 and WUS expression in response to profilin overexpression
It is apparent that spatial alteration in CLV1 kinase gene is associated with plant development. More than 30 % down-regulation in the transcript level of CLV1 in the floral tissues than the vegetative tissues of both Pf-Ox4 and Pf-Ox17 overexpression lines was observed. This radical alteration in the expression level of such important kinase gene indicates toward its direct role in controlling the apical meristem organization, differentiation and ultimately to the plant phase change. If so, are receptor-kinase genes including CLV1 receptor-kinase expressed in tight coordination with structural proteins such as profilins? Would the organization of apical-meristem in crop plants be altered by manipulating the expression patterns of profilins? In this direction, experimental validation of other important genes earlier reported for their role in the meristem organization and differentiation such as KNOX, LFY, WUS and ABP1 is required to confirm the genetic control and interaction relationships with profilin during plant development.
Yadav et al.  have reported that WUS protein is abundant in the neighboring cells of the apical meristem central zone and directly controls the transcriptional activation of CLV3 through binding to its promoter region. Consecutively, up-regulated CLV3 gene negatively regulates the WUS protein gradient across meristematic zone which is required for the regulation of stem cell number during floral determinacy. Multiple sequence alignment of CLV3 gene sequence of Arabidopsis with CLV1 gene sequence of tobacco showed high sequence homology and has shown congruency in their expression patterns. As shown in Fig. 3, the CLV1 expression level was increased in the vegetative tissues of both Pf-Ox4 and Pf-Ox17 lines than the reproductive tissues, and conversely, WUS expression was reduced in the floral-buds. This reduction in WUS expression is considered important for maintaining the low CLV1 expression level after the formation of floral meristem. It is evident that deletion of CLV3 promoter region containing WUS-binding sites leads to significant reduction in the promoter activity . Therefore, WUS controls the CLV1 expression in GhPRF1 overexpression lines during floral meristem transition, as proposed in Fig. 11. Taken together, this analysis demonstrated that WUS-mediated CLV1 transcription is maintained in the apical and floral meristems, aiding the early flower initiation in the overexpression lines.
Overexpression of GhPRF1 and expression dynamics of key flowering time controlling genes
Profilin genes have been characterized for their contribution to the cell wall organization catalyzing the key step of actin polymerization and depolymerization [71, 72]. Overexpression of profilin in Arabidopsis showed longer roots and root hair, broader leaf and accelerating the instigation of flowering [35, 54, 55]. Transient expression of profilin in tobacco cells exhibited extensive cell wall extensions, whereas developmental phases were radically influenced in the cotton fiber cells . A mutated profilin gene in Arabidopsis has reduced plant height, leaf size and delayed flowering. In particular, a mutant of prf1 or prf2 had defects in rosette leaf morphology and inflorescence architecture, whereas mutant of PRF3 led to plants with slightly elongated petioles. However, when the mutant plants were complemented with profilin, retention of normal phenotype was observed . By comparing the overexpressed profilin showing elongated roots, increased leaf size and accelerated flowering and subsequent reduction of these traits in prf-mutant could reveal valuable information about profilin functioning. However, little is explicit about their role in the complex genetic network which is required for apical meristem activation. In the present study, ectopic overexpression of trans-GhPRF1 in transgenic Pf-Ox4 and Pf-Ox17 lines demonstrated the hyperactivation of apical meristem and developmental reprogramming targeting early flowering time phenotype. Conversely, depletion of profilin in RNAi lines led to delayed flowering time phenotype and also in flower number per plant.
Concurrently, several transcription factors [46, 73, 74], auxin binding factors , peptides [65, 76] and signalling receptor-like kinases  are known for their involvement in meristem identity and maintaining balance between cell proliferation and organ formation at shoot/flower meristems  (Table 1). The process of flower initiation and its regulation is governed by a complex genetic network where important flower transition and flowering time controlling genes interact during flower development. These observations show that profilin-mediated expression alterations of key negative and positive flowering regulators occur in a coordinated manner. Hence, the hyperactivation of apical meristem and its early conversion into floral meristem is mainly due to increased expression level of positive regulators such as AP1, SOC1 and FT4 gene transcription which is a probable outcome of enhanced profilin content in the transgenic overexpression lines. During early onset of flowering, FT4 gene is required to activate AP1 expression which promotes other functioning genes required for floral meristem differentiation . It is clear with transcription data that FT4 gene was up-regulated in the vegetative tissues of the overexpression lines which would be transmitted to shoot apex for its morphological transformation into floral primordium . Apparently, AP1 gene transcription is similar in the 110 dpt and 100 dpt reproductive tissues of both control and Pf-Ox4 lines, respectively (Fig. 7a). But early expression elevation in AP1 gene, perhaps in response to up-regulated FT4 gene, resulted into early flowering in the overexpression lines than control plants. This is attributed to AP1 transcription, without which plants showed delayed flowering in Arabidopsis . Besides, GA pathway is also required for the activation of important trans-factors such as SOC1 gene along with the suppression of negative flowering regulator FLC1 gene , which is consistent with the expression data.
Therefore, with the over-expression of profilin gene in Pf-Ox4 and Pf-Ox17 lines, the early flowering phenotype is induced by ARPs-mediated modulation of key regulators. However, these genes could only promote flowering transition if a simultaneous expression reduction occurred in the negative regulators [79, 80]. As proposed, FLC1 and FT1 genes negatively regulate SOC1 trans-factor and FT4 genes which collaboratively results into early flowering (Fig. 11). The FT-family members are the positive flowering regulators [49, 81], however, FT1 member of tobacco has been shown to down-regulate key flower controlling genes such as SOC1 and others .
Apart from flowering time modulation, leaf size was also increased in the overexpression Pf-Ox4 and Pf-Ox17 lines. The size of leaves is firmly controlled by ecological and genetic factors controlling cell expansion and cell division mechanism in a spatial and temporal manner . The increased leaf dimensions observed in the overexpression lines suggested that profilin up-regulation may have influenced the intermediary genetic switches such as AVP1, GRF5, JAW, BRI1, and GA20OX1 genes . Whereas, overexpression of APC10, led to increased rate of the cell cycle and produced bigger leaves . Overexpression of ARGOS, the homologous protein of ORGAN SIZE RELATED PROTEIN1 increased the leaf size in plants by elongating proliferative phase of development . However, no report supported the interaction of these genes with temporal expression of profilin protein in the cell. Therefore, it will be of interest to perform the experimental validation of such genes responsible for regulating the leaf dimensions. In the current study, trichome density on the leaf surface and mid- rib of Pf-Ox4 and Pf-Ox17 lines highlighted for an extra elongation of epidermal cells contributing to the increased leaf size. Hence, increased leaf size and biomass has commercial aspects for several crop plants where leaves are the major source of human usages, such as tobacco, spinaches, cauliflower and more.
In the present study, the overexpression of GhPRF1 altered the expression of candidate flowering genes, indicating for its role in the intricate mechanism of flower development. However, it is still unclear how profilin regulates the expression of flowering time controlling genes in a coordinated manner? As noted elsewhere, profilin directs several signalling cascades via PIP and PIN genes which affect auxin concentration and its downstream signalling through polar transport of auxin during floral development . It is also clear that polar auxin transport and downstream signalling is essential for instigation of floral primordia and floral development [42, 87].
Profilin-mediated regulation of ARPs upstream to the flowering gene expression cascade
Flowering process in plants is a complex mechanism and profilin seems to influence factors involved in floral development. So its impact on expression alteration of key flowering genes might also be controlled by few unknown intermediary components. These intermediary molecules/pathways downstream to profilin act either independently or in an overlapping fashion. For example- i) AUXIN BINDING PROTEIN1 (ABP1) that acts as binding site for different auxin responses during cellular expansion, cell cycle and cytoskeletal rearrangements, ii) PIP signalling genes controlling vesicle trafficking and membrane-cytoskeleton dynamics, channel protein behaviour and signal transduction, and iii) Actin-Related Proteins (ARPs) which in proximate association with profilins control actin-nucleation and involved in flower induction and development, transcriptional re-programming at cellular level and cytoskeletal processes.
A parallel outlook of profilin function towards flowering is through ARPs, as there are reports suggesting their role in flower development [88, 89]. The ARPs act as epigenetic regulator through chromatin remodelling and promotes histone biosynthesis and modification, hence promoting FLC transcription . Different plant ARPs with their diverse functions as mentioned in Table 2, were considered for the characterization of their role in the observed flowering phenotype of profilin overexpression lines. Interestingly, ARP4 and ARP6 were down-regulated in the floral tissues than the vegetative tissues. These ARPs have been reported for their direct role in the regulation of flower development especially in defining flowering time phenotype. It has been shown that ARP4 gene regulates flower development by the modulation of chromatin structure, as the silencing of ARP4 gene led to early flowering in Arabidopsis . It is also evident that profilin polymerizes actin in association with ARP genes. Different ARPs have their roles in flowering phenomenon, for example, ARP6 induces FLC expression and its accumulation led to repression of flowering [88, 89]. Such coordinated regulation of flowering time has been characterized based on the molecular interaction of ARP4 and ARP6 genes with negative flowering regulator FLC1 gene. The FLC1 gene is positively regulated by ARP4 and ARP6 genes, that in result modulate flowering genes’ expression cascade. Among known ARPs, ARP4 and ARP6 are important for their role in flowering time phenotype as the % silencing of both these genes led to early flowering and flowering time phenotype in proportion [90, 91]. This information was further bolstered by the observation that ARP4 and ARP6 silencing lines exhibited a radical reduction in FLC1 gene transcription levels and uphold their hierarchy in the flowering mechanism . Evidently, ARP4 and ARP6 have direct control over vegetative to floral transition during the inception of flowering. Down-expression of both ARP4 and ARP6 genes’ transcription in profilin overexpression lines highlighted a mechanistic link to the functional aspects of profilins upstream to the apical-to-floral meristematic gene expression cascade. The present study shows the down-regulation of ARP genes in response to overexpressed profilin during developmental phase-change of plants. These findings suggest that profilin is the upstream regulator of ARPs in cellular milieu during floral induction (Fig. 11).
The present study implicates profilin responsive gene network as being involved in the progression of an early flowering phenotype. We provide clues here into flower initiation and developmental genes that may have been up-regulated directly by profilin or via other intermediates in profilin over- and down-expression transgenic lines. Notably, the up-regulation of meristem determinacy CLV1 gene and its regulatory WUS homeodomain trans-factor are enhanced in the vegetative tissue (apical meristem), as a result of the conversion of apical-to-floral meristematic tissue. This suggestion that expression up-regulation of key meristem determinacy floral induction genes through ARPs controlling flowering time and flower number, was primarily concomitant with the profilin-mediated metabolic transformation of the meristematic cell. This information is further bolstered by the remarkable observation that both independent profilin over-expression and RNAi transgenic stocks had conversely influenced these traits. These observations are mostly veritable at phenotypic, or perhaps at metabolic level highlighting their genesis to be congruent with developmental expression re-arrangements. An exciting prospect for future work will be to dissect the physiological dissimilarities generated by the interacting constituent genes into profilin overexpression and RNAi lines, and to learn about their altered regulation or expression. It would also be interesting to investigate if comparable expression patterns of flower controlling genetic network required for meristem conversion and further development are accompanied in other crops and for other traits, for example, enhanced plant vigor and resistance or tolerance to stress conditions.
Maintenance and generation advancement of tobacco plants
Seeds of tobacco (Nicotiana tabacum L.) cultivar Xanthai were sown in 1:1 mixture of soil : soilrite. The germinated healthy seedlings of individual plantlets were grown in sterilized soil mix in the University Green House at 32 ± 1 °C with 16 h light and 8 h dark photoperiodic conditions. Flowers were bagged and tagged for generation advancement in the green house after 110-115 dpt. Along with seeds of Xanthi were grown in vitro by surface sterilization and germination on MS medium  at 28 °C ±1 °C with 16 h light and 8 h dark photoperiodic conditions. The control plants were maintained in vitro by regular sub-culturing of nodal explants on the MS medium. These control plants were subsequently transferred to the 1:1 mixture of soil : soilrite for hardening and growth.
Gene construct design for GhPRF1 overexpression
Full- length GhPRF1 cDNA was amplified by PCR using oligonucleotide primer set (Additional file 10) and Q5 polymerase (New England Biolabs) from the cotton cotyledonary leaf. In brief, total RNA was isolated from cotton leaf tissues using Qiagen RNeasy plant mini kit as per the manufacturer’s recommendations. Isolated RNA sample was quantitatively and qualitatively assessed by Nanodrop (Thermo Scientific). One microgram of total RNA was used for synthesizing the complementary DNA using QuantiTect reverse transcription kit (Qiagen). Using Q5 DNA polymerase (NEB), PCR amplification was performed with synthesized cDNA as template and primers as mentioned in Additional file 10, following standard thermal conditions. Gel electrophoresis of the PCR reaction was performed and the amplified product of profilin gene (402 bp) was eluted using Qiagen Gel extraction kit. This amplicon was first sequenced and then used to develop binary vector construct. The PCR product was cloned into the NcoI and BamHI restriction sites in the pPRT100 cloning vector downstream of the CaMV 35S promoter. The complete cassette of 35S: GhPRF1:pA was excised out as a HindIII fragment and cloned into HindIII restriction site of binary vector pPZP200, along with nos:nptII gene cassette as a plant selection marker. The modified binary vector was electroporated in Agrobacterium tumefaciens strain GV3101.
Gene construct design for GhPRF1 silencing
Full- length sense and antisense strands of profilin gene consisting of 402 bp coding for 133 amino acid protein were amplified from the cotyledonary leaf of cotton using specific primers having restriction sites optimized for its cloning into pHANNIBAL Plasmid  by XhoI, EcoRI and XbaI, BamHI sites, respectively. Following this method, pHANNIBAL plasmid was developed containing profilin sense and inverted antisense strand sequences flanking intronic region. 35S:PRF-intron-FRP region from pHANNIBAL plasmid was digested out using NotI restriction enzyme and the sticky ends were polished using HF-Polymerase enzyme. Subsequently, this region was ligated to pPZP200 binary vector in SmaI restriction site and the pPZP200nos:nptII:pA::35S-PRF-intron-FRP:pA gene construct was developed. This GhPRF1 silencing construct was further electroporated in Agrobacterium strain GV3101 by using GenePulsar (BioRad).
Gene construct design for GUS gene
The gene sequence of β-glucuronidase (gus) gene was cloned into pPZP200 binary vector plasmid under the control of CaMV35S promoter, along with nos:nptII:pA gene cassette as a plant selection marker. The modified binary vector was electroporated in Agrobacterium tumefaciens strain GV3101.
Development of transgenic tobacco with specific gene constructs
Xanthai leaf explants were used for Agrobacterium-mediated genetic transformation following the standard protocol . Transformed explants tissues were allowed to undergo in vitro organogenesis on MS medium supplemented with auxin (NAA = 0.1 mg/l), cytokinin (BAP = 1.0 mg/l) and selection marker kanamycin (100 mg/l). Several putative transgenic shoots were harvested and inoculated on hormone-free MS medium in the test-tubes supplemented with kanamycin (100 mg/l) for at least three successive sub-culturing in vitro. After 30–35 days of shoot growth in vitro, the independent putative transgenic lines were transferred to soil in the green house for hardening and plants were maintained for their appropriate growth and development under controlled conditions.
Genomic DNAs of putative transgenic lines were extracted from leaf tissues using DNeasy DNA isolation kit (Qiagen). Using nptII gene-specific primers (Additional file 10), PCR was employed to screen the putative transformants carrying nos:nptII and 35S:GhPRF1 gene cassettes. Confirmed transgenic shoots having GhPRF1 transgene were grown for at least three rounds of sub-culturing on selection medium (kan 50 mg/l). Plantlets grown on selection media were successfully hardened in the green house and considered for expression analyses. PCR analysis for the expression pattern of the GhPRF1 gene was performed in 23 transgenic plants. The two lines showing high GhPRF1 expression (Pf-Ox4 and Pf-Ox17) and one RNAi line (Pf-Si23) were selected for analysis.
Expression analyses of transgenic lines
PCR positive transgenic lines were considered for the analysis of profilin expression level through RT-PCR. Total RNA was extracted from leaf and flower-bud tissues of 4-week old Xanthi wild-type seedlings using RNeasy plant kit (Qiagen) according to the manufacturer’s protocol. The RNA samples having a concentration of at least 1.0 μg were reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s protocol. The replicated RT-PCR was performed using profilin gene-specific primers (Additional file 10) in the leaf and flower tissues of wild type Xanthi plant and PCR positive 35S:GhPRF1 overexpression lines. The amplified products were subsequently electrophoresed on 1 % agarose gel and observed under UV illumination for relative quantification of transcripts. Normalization of quantitative gene expression data was performed by using previously optimized GAPDH and L25 genes as an internal reference gene for different tobacco tissues [93, 94].
Morphological analysis of transgenic lines
Transgenic lines confirmed for transgene integration were established in the green house. At regular intervals during vegetative and reproductive phases of plant growth, independent transgenic lines along with control plants were measured for total plant height, internode length, internode number, leaf sheath and leaf lamina length/width, number of flower, size of flower, flowering time, trichome patterning, trichome density and biomass yield. The anatomical features were examined through transverse sections of several vegetative and floral tissues including leaf, stem, petiole, bracts, petals and ovary using Olympus SZ61 microscope.
Aniline blue staining test of pollen grains
The pollen viability of overexpression, down-expression and control plants was assessed by an aniline blue test. In brief, the mature pollen grains were harvest in the morning at least after 2–3 h of anthesis in at least three biological replicates of each transgenic or control plant. The pollen grains were placed on a glass slide and immersed into diluted aniline blue solution (aniline:water = 1:1). The pollen grains were analysed under Olympus SZ61 microscope and counted for the viable and non-viable pollen grains per optical view of different biological replicates.
Ethics approval and consent to participate
Consent for publication
Availability of data and materials
All the data and materials supporting our research findings are contained in the methods section of the manuscript. Also, details are provided in the supplementary data attached with the manuscript.
days post transplantation
milligrams per liter
Murashige and Skoog
micrograms per liter
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Authors are thankful to the Department of Biotechnology (DBT), Government of India for the financial support to carry out this research work. Authors are also thankful to the CSIRO, Australia for providing pHANNIBAL plasmid DNA. Authors are also thankful to Prof. Deepak Pental, University of Delhi South Campus, New Delhi INDIA for providing tobacco seeds, pPRT100 cloning vector, pPZP200 binary vector and Agrobacterium tumefaciens strain GV3101. The technical support of Ms. Payal Nigam is also acknowledged.
This research work was supported by the Department of Biotechnology (DBT), Government of India. Research fellowship of D.K.P. was also supported by the DBT, Government of India.
The authors declare that they have no competing interests.
BC conceived the original research project. BC supervised the experiments; DKP performed all the experiments; BC and DKP wrote the manuscript. Both the authors have approved the final manuscript for publication.
Mr. Dhananjay Kumar Pandey, School of Biotechnology, Gautam Buddha University, Greater NOIDA INDIA.
Dr. Bhupendra Chaudhary, School of Biotechnology, Gautam Buddha University, Greater NOIDA INDIA.
Floral dimensions in transgenic Pf-OX4 line. (PPT 123 kb)
Statistics of flower phenotype of Pf-Ox4 transgenic lines in comparison to control plant. (PPT 184 kb)
Comparative analysis of internode length at 13th and 20th node of Pf-Ox4 and control plant showing differences at 13th -14th internode. (PPT 150 kb)
Comparative analysis of vegetative tissues showed increased leaf dimensions in Pf-Ox4 transgenic plant in comparison to control tobacco plant. (A) Leaf dimensions of 13th leaf of Pf-Ox4 and control. (B) Leaf dimensions of 20th leaf of Pf-Ox4 and control plant. (PPT 160 kb)
Trichome density at the leaf margin and midrib of overexpression line (13th leaf from top). (A) Trichome density on mid-rib of Pf-Ox4; (B) Trichome density on mid-rib of control leaf; (C) Trichome density on the margin of Pf-Ox4 leaf; (D) Trichome density on the margin of control leaf. (PPT 2485 kb)
Multiple sequence alignment of six cotton profilin genes showing high homology among the genes. (PDF 150 kb)
Nucleotide sequence analysis of six profilins in cotton showing occurrence of different nucleotides and their percentage frequencies. (PPT 154 kb)
Gene structure of Nt PRF, At PRF and Gh PRF showing three exonic regions intercalated by intron sequence (Gene structure display server, Ver 2). (PPT 136 kb)
Pairwise sequence alignment of tobacco and cotton native profilin protein using Blosum 62 algorithm with gap and extended penalty 10 and 0.5 respectively showing 88.6 % similarity with score value 589.5. (PPT 177 kb)
Primer sequences used in the study. (PPT 207 kb)