Fig. 6
Genetic manipulation of profilin gene in tobacco. a Transgenic plants are developed with ectopic constitutive overexpression line, constitutive down-expression line, and control line, respectively. All three lines are photographed at the same age. The inset picture shows the relative expression of proflin gene in control, overexpression, and RNAi silencing line. b Flower number per plant is shown for Pf-Ox4 line. c Flower number per plant is shown for the Pf-Si23 line. d Flower number per plant is shown for control line. e A number of flowers produced by Pf-Ox4 and Pf-Si23 transgenic lines along with control plant. One way ANOVA analysis was performed for statistical analysis of differences using Graphpad Prism resulted in R2 value 0.9467. f The onset of flowering in Pf-Ox4 and Pf-Si23 transgenic lines along with control plant after transplantation. Unpaired t-test was performed (p < 0.05) to analyze significant differences in flowering time