- Research article
- Open Access
Direct purification of detergent-insoluble membranes from Medicago truncatularoot microsomes: comparison between floatation and sedimentation
© Guillier et al.; licensee BioMed Central Ltd. 2014
- Received: 27 May 2014
- Accepted: 20 September 2014
- Published: 30 September 2014
Membrane microdomains are defined as highly dynamic, sterol- and sphingolipid-enriched domains that resist to solubilization by non-ionic detergents. In plants, these so-called Detergent Insoluble Membrane (DIM) fractions have been isolated from plasma membrane by using conventional ultracentrifugation on density gradient (G). In animals, a rapid (R) protocol, based on sedimentation at low speed, which avoids the time-consuming sucrose gradient, has also been developed to recover DIMs from microsomes as starting material. In the current study, we sought to compare the ability of the Rapid protocol versus the Gradient one for isolating DIMs directly from microsomes of M. truncatula roots. For that purpose, Triton X-100 detergent-insoluble fractions recovered with the two methods were analyzed and compared for their sterol/sphingolipid content and proteome profiles.
Inferred from sterol enrichment, presence of typical sphingolipid long-chain bases from plants and canonical DIM protein markers, the possibility to prepare DIMs from M. truncatula root microsomes was confirmed both for the Rapid and Gradient protocols. Contrary to sphingolipids, the sterol and protein profiles of DIMs were found to depend on the method used. Namely, DIM fractions were differentially enriched in spinasterol and only shared 39% of common proteins as assessed by GeLC-MS/MS profiling. Quantitative analysis of protein indicated that each purification procedure generated a specific subset of DIM-enriched proteins from Medicago root microsomes. Remarkably, these two proteomes were found to display specific cellular localizations and biological functions. In silico analysis of membrane-associative features within R- and G-enriched proteins, relative to microsomes, showed that the most noticeable difference between the two proteomes corresponded to an increase in the proportion of predicted signal peptide-containing proteins after sedimentation (R) compared to its decrease after floatation (G), suggesting that secreted proteins likely contribute to the specificity of the R-DIM proteome.
Even though microsomes were used as initial material, we showed that the protein composition of the G-DIM fraction still mostly mirrored that of plasmalemma-originating DIMs conventionally retrieved by floatation. In parallel, the possibility to isolate by low speed sedimentation DIM fractions that seem to target the late secretory pathway supports the existence of plant microdomains in other organelles.
- Detergent insoluble membrane
- Plant microdomain
- Medicago truncatula
Biological membranes that compartmentalize cells into organelles or form a barrier to the outside environment are composed of lipids as well as a variety of trans-membrane, lipid-modified and lipid-associated proteins essentially involved in transport, signaling, differentiation and stress adaptation processes. Aside from the fluid mosaic model that refers to a homogenous distribution of lipids and proteins within the plasma membrane (PM) , a large body of evidence supports the microdomain hypothesis , stating that membranes are also compartmentalized by uneven distributions of specific lipids and proteins into microdomains termed membrane rafts. Originally characterized in animal and yeast cells, membrane rafts are defined as plasma membrane  nano- or microdomains enriched in sphingolipids and sterols, which act as platforms initiating signaling events in diverse physiological situations, including inflammation processes and apoptotic cell death . The main hypothesis relative to the functional significance of these domains relies on the lateral segregation of membrane proteins that creates a dynamic scaffold to organize particular cellular processes . In plants, sphingolipid- and sterol-enriched membrane microdomains were also isolated from PM. Characterization of their protein content revealed their enrichment in proteins involved in signaling and response to biotic/abiotic stresses -, suggesting that plant membrane microdomains may exert similar signaling functions to their animal counterparts.
Main literature background to microdomain preparations as related to initial fractions
DIM recovery process
Root callus cultures
Leek and Arabidopsis
GA, ER, PM
Oat and Rye
Whether this result also holds true for plants of agronomic has not been investigated yet, despite the recognized importance of membrane microdomains during plant-microbe interactions (reviewed in ,). Although Medicago truncatula has been retained more than ten years ago as the model for studying legumes and root symbiotic interactions with fungi and bacteria , only one report has been dedicated to the analysis of DIM fractions in barrel medic . The study showed that membrane raft domains corresponding to Triton X-100 insoluble membranes could be obtained from M. truncatula root PM. Additionally, evidence was given for their enrichment in proteins associated with signaling, cellular trafficking and redox processes. A raft protein termed Symbiotic REM (MtSYMREM1, or MtREM2.2)  was also found to control Sinorhizobium meliloti infection as well as rhizobial release into host cell cytoplasm within root symbiotic structures, the so-called nodules . Likewise, Haney and Long  identified two microdomain-associated plant flotillins required for infection by nitrogen-fixing bacteria. These data raise the possibility that rafts may be involved in molecular events leading to successful nodule onset, and it is tempting to speculate that additional symbiotic associations like mycorrhiza may also require proper raft structures for their establishment and functioning. Elucidating microdomain function(s) in symbiosis and legume physiology thereby implies increasing knowledge about their cellular distribution coupled to fast and efficient methods dedicated to their isolation.
Although DIM fractions have been successfully prepared from M. truncatula root tissues using PM as starting material , this protocol requires a huge amount of root tissues. Additionally, purifying PM fractions turns out to be somehow labor-intensive and time-consuming. To overcome these technical limitations together with enlarging the coverage of DIM populations in legume roots, we investigated in the current study an alternative that relies on the possibility to skip the PM fractionation step, to isolate DIM fractions directly from microsomes, as previously described in other animal and plant model systems (Table 1). This work was thus intended to purify microdomains directly from M. truncatula root whole cell membranes by comparing two fast protocols previously described for DIM purification ,. Using roots of soil-grown M. truncatula plants as starting material, we first analyzed the impact of detergent final concentration and detergent/protein ratio on lipid and protein patterns of DIM fractions. We then selected specific experimental conditions and used a GeLC-MS/MS proteomic approach, where biological samples are separated by SDS-PAGE, sliced, digested in-gel and analyzed by LC-MS/MS, on the DIM fractions retrieved from the two distinct protocols. Respective DIM protein populations were further contrasted with regard to their functional and cellular distributions.
Purification of DIMs from M. truncatula root microsomes
Sterols, but not sphingolipids, are differentially enriched between R- and G-DIM fractions
As long-chain base (LCB) represent a common backbone to all sphingolipids, they were quantified by GC-MS  as a way to access the total enrichment in sphingolipids in R- and G-DIM fractions. Whatever the method used for DIM preparation, the resulting total LCB composition (Figure 2C) was consistent with previously data reported for M. truncatula, even though there was evidence for additional minor dihydroxylated LCB (d18:0, d18:1 and d18:2), the detection of which was previously ascribed to the high sensitivity of GC-MS . Interestingly, both R- and G-fractions were highly enriched in trihydroxylated LCB (c.a. 6-fold increase in t18:0 and t18:1 when compared to Mic). These compounds are mainly found amidified in the sphingolipid class of glycosyl-inositolphosphoryl-ceramides . Additionally, R- and G-samples also exhibited a very similar LCB profile with identical enrichment-folds whatever the TX-100 concentration used (Additional file 1: Additional A1B), strongly suggesting that sphingolipid content is not dependent on the method used for DIM isolation. Overall, the lipid composition of R- and G-DIMs confirmed their enrichment in sphingolipids and sterols relative to the microsomal fraction.
DIM protein composition is impacted by the extraction method
To go further in analyzing and comparing the proteins co-extracted with sterol-enriched DIM fractions, a shotgun proteomic approach was performed using the original setup for TX-100 concentrations, namely R3:1 and G3:2 conditions after admitting the detergent-independence of DIM lipid and protein composition over this range. Due to the limitations of two-dimensional electrophoresis to resolve integral membrane proteins , 1D gel coupled to liquid chromatography-tandem mass spectrometry (GeLC-MS/MS) was chosen to investigate the protein composition of DIM fractions. This workflow that combines a size-based protein separation to an in-gel digestion of the resulting fractions proved to be successful in expanding the coverage of membrane proteins in M. truncatula roots ,, and is also amenable to relative protein quantification methods such as spectral counting .
GeLC-MS/MS was thus conducted on two independently-extracted sets of R- and G-DIMs and the initial root microsomal fraction. Using a probability of peptide misidentification inferior to 0.05, a total of 874 non redundant proteins were overall identified in the microsomal and DIM fractions when retaining only those co-identified in the two replicates of each DIM, as listed in additional data (Additional file 2: Table A1). The Venn diagram distribution of microsomal, R- and G-DIM proteins, displayed in Figure 3B, indicated that relative to the 821 accessions initially identified in the microsomal fraction, R- and G-DIMs encompassed a rather similar number of proteins corresponding to 234 and 219 accessions, respectively. Although most of DIM-associated proteins (84%) were as expected also present in the original microsomal fraction, 53 accessions (16%) were uniquely identified in DIMs, indicating that the experimental procedure has enabled the identification of minor proteins that have escaped detection during mass analysis of whole membranes but are revealed upon fractionation. Noticeably, comparison of R- and G-DIMs showed that a common pool of 126 proteins was shared between both fractions, thereby defining a conserved core-set of DIM-associated proteins that overall represented 15% of the root microsomal proteome of M. truncatula.
To investigate whether there might be a difference in the quantitative distribution of these common proteins between R- and G-DIMs, protein abundance was estimated using spectral counting, which is based on the cumulative sum of recorded peptide spectra that can match to a given protein . Following the calculation of a normalized spectral abundance factor (NSAF) value for each protein across the four replicates, only six proteins displayed a significant (p < 0.05) differential accumulation between R- and G-DIMs (Figure 3C). Namely, a mitochondrial import receptor subunit TOM40 homolog, a fasciclin-like arabinogalactan protein and a hexokinase displayed a higher abundance in R-DIMs than in G-DIMs, whereas an elongation factor 1-alpha, a V-type proton ATPase subunit H and an asparagine synthetase over-accumulated in G-DIMs relative to R-DIMs. As a result, the 126 proteins shared between both fractions largely corresponded to a quantitatively conserved set of DIM-associated proteins irrespective of the extraction method, in which the top 10 major abundant proteins included transmembrane porins (aquaporins, OMP), respiratory chain related proteins (ATP synthases, flavoprotein), beta-glucosidase G1 and ubiquitin, as very often described in plant DIM fractions (Additional file 3: Table A2) ,. On the opposite, the Venn diagram also showed that out of the 234 and 219 proteins identified in R- and G-fractions, 108 (46%) and 93 (42%) proteins were unique to R- and G-DIM, respectively (Figure 3D). This pointed out that 61% (201 proteins) of the 327 DIM-associated proteins in M. truncatula roots underwent a differential partition according to the purification procedure. Consequently, even though both approaches had equivalent protein extraction efficiencies, as inferred from the similar number of accessions identified in R-and G-fractions, they nonetheless displayed a differential selectivity toward microsomal proteins.
DIM-enriched proteins differ between R- and G-fractions
To further assess the extent to which protein composition of R- and G-DIMs quantitatively differed from that of initial microsomes, an abundance ratio between NSAF values of DIM and Mic fractions was calculated for each protein. On this basis, accessions that reproducibly displayed at least a 2-fold higher abundance in R- and G-DIMs than in microsomes were considered as DIM-enriched proteins according to Borner’s sensu. Among them, 65 were unique to R-DIMs (fraction termed “R2xspecific”) and 46 were unique to G-DIMs (fraction termed “G2xspecific”), whereas 42 were shared between R- and G-DIMs (fraction termed “RG2xcore”) (Figure 3D). From these results, it was thus concluded that each extraction procedure generated a specific subset of DIM-enriched proteins from Medicago root microsomes, which accounted for 7.4 and 5.3% of the initial 874 identifications, for R- and G-protocols, respectively. The rest of study was thus essentially dedicated to the comparison of these two specific proteomes and the core subset, relative to the microsomal fraction.
When investigating the representation of previously published plant DIM-associated proteins within our proteomic data by using identification mapping tools and homology search against the protein listed in ,,,,, and , 152 proteins already described in plant microdomains were identified within the total 327 R- and G- proteins, including 33 proteins usually referred to as canonical plant DIM markers in the literature such as remorin (Additional file 3: Table A2). Noticeably, 14 DIM markers were overall identified within DIM-enriched Medicago proteins, which encompassed fasciclin-like arabinogalactan proteins, hedgehog-interacting protein, receptor-like kinases, 14-3-3 like protein, phospholipase D, dynamins, and flotillin. However, their distribution remarkably differed between R2xspecific and G2xspecific subsets (Figure 3E), thereby comforting the view that R- and G-approaches displayed a differential selectivity toward certain classes of proteins.
Finally, to address whether known or putative non-membrane proteins might be enriched in R- and G- DIM fractions, we used, as a point of reference for M. truncatula, the rationale described by Daher and co-workers  that favors similarity search on the basis of which homologous proteins share the same location in many organisms, a strategy recognized more confident than the use of in silico algorithmic predictors for protein localization . Consequently, DIM-enriched proteins obtained from R- and G-protocols were first compared with BLASTP to TAIR database accessions and were considered as membrane M. truncatula proteins when homologous sequences displaying at least 70% pair-wise identity and a cut-off expectation value of e-40 were experimentally demonstrated to have a membrane localization, including core integral or subunits of membrane complexes, on the basis of direct assays . In the absence of TAIR homologues, LegumIP annotations that overall agreed up to 80% with Arabidopsis-inferred cellular components, even though largely less detailed, were used to address protein localization (Additional file 3: Table A2). In the absence of confident membrane homologues, DIM-enriched proteins were retained as non-membrane proteins unless predicted to display at least one of the following criteria: to form an alpha helical TM domain or a beta barrel embedded in the membrane lipid bilayer, to be anchored to the membrane owing to hydrophobic tails, and/or to be targeted to the secretory pathway, as previously described ,. Using this design, 10 accessions mainly of cytosolic origin, out of the total 227 proteins previously recorded as DIM-enriched were identified as potential contaminants of membrane fractions (Additional file 3: Table A2). However, when considering their known or putative functional relevance in microdomain formation with special regard to role in mediating hydrophobic interactions and/or responses to microbial ingress/accommodation at the interface of plant-microbe interactions that largely depend on exocytocis, endocytosis, or local secretion of defense compounds , we made the deliberate choice not to discard them from R- and G-DIM fractions. Namely, patellin-5 binds to hydrophobic molecules such as phosphoinositides and promotes their transfer between different cellular sites. The PLAT/LH2 family domain of lipase/lipoxygenase is found in a variety of membrane or lipid associated proteins, and dynein transports various cellular cargo by walking along cytoskeletal microtubules. Ubiquitin, linkage of which to PM proteins is known to induce endocytosis and/or proteasome-dependent degradation , whereas caffeic acid 3-O-methyltransferase is involved in the reinforcement of the plant cell wall and in the responding to wounding or pathogen challenge by the increased formation of cell wall-bound ferulic acid polymers. Major latex proteins belong to cytokinin-specific binding proteins that also have role in pathogen defense responses. Sorting and assembly machinery component 50 (cell division protein FtsZ homolog) is part of a ring in the middle of the dividing cell that is required for constriction of cell membrane/cell envelope and localizes to very-long chain fatty acids-containing phospholipids that have an important role in stabilizing highly curved membrane domains ,. Finally, glycoprotein-binding proteins (lectins) have been suggested to contribute to stimulus-dependent microdomain assemblies via cross-linking of PM-resident proteins ,.
Taken together, the above data confirmed that both the Rapid (R) and Gradient (G) protocols enabled the isolation of microdomain fractions directly from M. truncatula root microsomes, as inferred from sterol enrichment, presence of typical sphingolipid long-chain bases from plants, enrichment in membrane proteins including well-known plant DIM reporters, but also showed that the method used for DIM extraction, namely low-speed centrifugation versus floatation, qualitatively impacted the composition of the proteome enriched in DIM fractions relative to initial microsomes. Consequently, to get a deeper insight regarding the processes by which DIM-enriched proteins may preferentially partition to either R- or G-DIM fraction, the corresponding M. truncatula proteins were further characterized with regard to their subcellular localization, functional relevance and features known to drive membrane association.
R- and G-DIM-enriched proteins differ in their cellular location
Keeping in mind that each frequency does not refer to an exclusive subcellular component and that frequencies may be biased toward the most studied Arabidopsis and legume organelles, it nonetheless appeared from Figure 4A that plasma membrane had the highest rate of occurrence within the RG2xcore proteome, a result that substantiates the view according to which the PM largely contributes to microdomain-enriched proteins . However, although proteins ascribed to mitochondrion were largely depleted in this core fraction relative to initial microsomes, as previously observed by Zheng et al., those located to other cellular components such as cell wall and non-green plastids happened to be enriched in Medicago root DIMs. Consequently, the subcellular profile obtained for this core fraction agreed with the idea that besides the plasma membrane, DIMs can be extracted from several other cellular compartments, as essentially demonstrated before for endomembrane systems when analyzing organelle-enriched fractions (Additional file 2: Table A1). In this respect, whereas the presence of plant cell wall-related proteins within DIM fractions has been widely reported in the literature ,, the retrieval of plastidial component in microdomains is far less documented. Nonetheless, Arabidopsis TOC75 protein, a component of the plastid outer membrane, was found in a fraction of detergent-insoluble membranes , supporting the idea that specific proteins might be included in microdomains of plastid membranes. In the current study, a beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ (Medtr2g008620), experimentally ascribed to the chloroplast envelope and the cell wall and reminiscent of the beta-hydroxyacyl-(acyl-carrier-protein) dehydratase precursor previously identified in M. truncatula DIMs , was enriched more than 50 fold in both R- and G-DIM fractions, relative to microsomes, Because this enzyme displayed no chloroplast transit peptide (cTP), but may be plastid-encoded according to HAMAP prediction (data not shown), it is likely that this protein that has role in lipid biosynthesis may serve specific function(s) at the plastid membrane. Likewise, phospholipase D alpha, a noticeable plant DIM marker that participates to the metabolism of phosphatidylcholines, which are important constituents of cell membranes, lipase/lipoxygenase, and patellin-5 (see above), also belonged to those lipid-related proteins co-enriched in R- and G-DIMs that can localize to non-green plastids (Additional file 3: Table A2). Regarding plastids, it is worth noting that these organelles are specialized, among other features, for the synthesis of fatty acid precursors that are either directly assembled within their own membranes, exported to the ER for extraplastidial lipid assembly, or reimported for the synthesis of plastidial lipids .
With special interest in those proteins specifically enriched in R- and G-DIMs relative to microsomes, the most remarkable differences recorded between the subcellular patterns of these two fractions included enrichment in proteins ascribed to cytosol/cell wall/undefined membrane components and a depletion of nuclear proteins in the R-specific subset, whereas plasmodesma- and nucleus-associated proteins were enriched in the G-specific fraction (Figure 4A). Among the 22 cytosolic proteins recorded as specifically enriched in R-DIMs, only 6 didn’t display any feature driving association to membranes and were exclusively assigned to cytosol according to experimental annotations, indicating that association of cytosolic proteins to R-DIM was not driven in the majority by non-membrane proteins. Likewise, all the 13 proteins located to the cell wall that were exclusively enriched in R-DIMs were predicted to have a membrane signature, as illustrated by germin-like protein, alpha-D-xylosidase, alpha-1,4-glucan-protein synthase, cysteine proteinase inhibitor 5, pectinesterase, beta-D-glucosidase, beta xylosidase, xylan 1,4-beta-xylosidase (Additional file 3: Table A2). It was also noticeable that R-procedure generated a DIM fraction largely depleted in nuclear proteins opposite to what observed for the gradient-based method, as previously depicted by Adam et al.. Although mainly consisting of ribosomal proteins, most of the nucleus-ascribed proteins specifically enriched during G-DIM isolation displayed at least a membrane-related feature, which overall minimizes the likelihood that free ribosomes may have stricken to the lipid fraction during our extraction procedures . Finally, plasmodesma-located proteins happened to be selectively enriched in G-DIMs, as inferred from the presence of 22 accessions ascribed to this compartment, although not exclusively, among which the DIM-marker flotillin belongs to (Additional file 3: Table A2). In plants, plasmodesmata correspond to membranous channels that allow intercellular communication. Embedded in the cell wall, they are defined by specialized domains of the endoplasmic reticulum and the plasma membrane, which may explain the large representation (Additional file 3: Table A2) of transporters and receptor-like kinase within the plasmodesma proteins enriched in G-DIMs, similar to what observed in the proteomic studies recently dedicated to plasmodesmata ,. Due to the relative specialization of each organelle toward protein sorting and/or particular metabolic pathways, we thereby anticipated that the differential distribution of R- and G-DIM-enriched proteins over distinct cellular compartments might be of functional relevance.
R- and G-DIM-enriched proteins differ in their functional relevance
Taken together, the above-data showed that despite the existence of a conserved core of proteins, R- and G-protocols each resulted in the enrichment of a particular DIM-proteome displaying specific cellular localizations and biological functions. Differences in protein and/or lipid associated with DIM fractions have been reported earlier, but essentially as dependent upon extraction parameters such as temperature, concentration and type of detergent ,. However, in the current study, DIM extraction procedures (temperature, duration, and detergent) were identical for both R and G protocols, which consequently only differed at DIM isolation process, namely sedimentation versus floatation on sucrose gradient. In this context, it was reasonable to assume that the selectivity displayed by each method could have arisen from some particular membrane compatible characteristics displayed by these differentially-enriched proteins.
Membrane-associative features as related to R/G protein partitioning
Among the features favoring protein embedment in the hydrophobic lipid bilayer are membrane spanning protein domains that include typically alpha-helices or beta-sheets with hydrophobic surfaces serving as the interface to the hydrocarbon core of the lipid bilayer. In addition to, signal peptides of nascent proteins can also mediate protein translocation across or integration to membranes along the secretory pathway . Protein association to membranes can also be driven by lipidic anchors, which can either be permanent co-translational additions or posttranslational modifications. These lipid modifications include glycophosphatidylinositol (GPI) anchors, N-terminal myristic acid tails (myristoylation), and cysteine acylation (palmitoylation) . Consequently, we further monitored and compared the putative presence of trans-membrane (TM) spanning domains, signal peptide (SP) sequences, and lipid modifications and within R- and G-enriched proteins, relative to microsomes, as inferred from the corresponding online predictor tools (see Methods).
In the current study, DIMs were prepared for the first time directly from M. truncatula root microsomes that consist of a complex membrane mix relative to the PM conventionally used as starting material for microdomain isolation. We clearly established that both long-lasting sucrose gradient centrifugation (G protocol) and rapid microfuge sedimentation at low speed (R protocol) enable the recovery of membrane fractions that meet the criteria of DIMs, as inferred from sterol enrichment, presence of typical sphingolipid long-chain bases from plants, and enrichment in membrane proteins including canonical DIM markers. Proteomic analysis of the corresponding fractions also show that, despite the existence of a conserved core of proteins, R- and G-protocols result in the enrichment of a particular DIM-proteome displaying specific cellular localizations and biological functions. Collectively, even though microsomes were used as initial material, we show that the composition of the G-DIM fraction still mostly mirrored that of PM microdomains conventionally retrieved by floatation. In parallel, the possibility to isolate by rapid differential centrifugation a DIM fraction that seems to target the late secretory pathway opens new avenues to study plant microdomains. Finally, with regard to our initial questioning addressing the intracellular distribution of plant DIMs, current results obtained in M. truncatula roots clearly support the existence of microdomains not only in PM and the late secretory pathway, but also in additional membrane organelles, including non-green plastids.
Medicago truncatula cv. Jemalong 5 seeds were surface-sterilized and germinated at 27°C in the dark onto 0.7% sterile agar . Two-day old seedlings were then transferred on soil and grown into 400 ml plastic pots containing a mix of sterile soil of Epoisses (neutral clay loam from Domaine d’Epoisses, INRA Dijon France) and sand (1:2, v/v) supplemented twice a week with a nitrogen-enriched nutrient solution (Long Ashton ) under controlled conditions (16 h photoperiod, 220 μE.m-2.s-1 light irradiance). After 4 weeks, roots were collected, gently rinsed with deionized water to get rid of soil, deep frozen in liquid nitrogen and stored at -80°C until further use.
Microsomal protein purification
All steps of microsome preparation were carried out at 4°C according to . Microsomes of M. truncatula roots were obtained by differential centrifugation as previously described for tobacco cells . Briefly, frozen roots (about 100 g fresh weight) were homogenized using a Waring Blender in grinding buffer (50 mM Tris-MES, pH 8.0, 500 mM sucrose, 20 mM EDTA, 10 mM DTT and 1 mM PMSF). The homogenate was successively centrifuged at 12.000 g and 16.000 g for 20 min. After centrifugation, supernatants were collected, filtered through two successive meshes (63 and 38 μm), and centrifuged at 100.000 g for 1 h. Microsomal pellets were resuspended in buffers according to the DIM fraction isolation procedure used (see below), homogenized with a glass pestle. Protein contents were quantified using the RCDC (bicinchoninic acid) Protein Assay Kit (BioRad) to avoid TX-100 interference, using BSA as standard.
Detergent-insoluble-membrane fraction isolation
For both the Rapid (R) and the Gradient (G) protocol (Figure 1), three independent extractions of DIMs were performed, each from a 6 mg protein equivalent of microsomes. Anotations R/G x:y were used to refer to the R or G protocol, the x detergent/protein ratio (w/w), and the y (%, v/v) final detergent concentration, respectively.
The rapid protocol was performed as described in . The microsomal fraction was first resuspended in 10 mM Tris-MES buffer pH 7.3, containing 250 mM sucrose, 1 mM EDTA, 10 mM DTT, 1 mM PMSF, 10 μg/ml aprotinin and 10 μg/ml leupeptin. To increase solubilisation, resuspended microsomal proteins were aliquoted into 1 mg fractions that were further diluted (270 μl final volume) with 25 mM Tris-MES pH 6.5, containing 150 mM NaCl. Then, 30 μl of 10% (v/v) Triton X-100 was added to reach a final detergent-to-protein ratio of 3:1 (w/w) and a 1% final detergent concentration (v/v). Incubation was performed on ice under gentle shaking for 30 min. Samples were centrifuged at 16.000xg for 20 min. The resulting Triton X-100-insoluble fraction (DIM) was homogenized (using a micropestle) in 200 μl of beta-octylglucoside-containing buffer (60 mM beta-octylglucoside, 10 mM Tris HCl pH7.5, 150 mM NaCl) and incubated on ice under gentle shaking for 30 min. A last centrifugation step (16.000 g for 20 min) was performed to separate soluble DIM fraction (R-DIM) from both Triton X-100 and beta-octylglucoside-insoluble pellet. Aliquoted DIM fractions were pooled and protein amount was measured using the RCDC Protein Assay Kit (BioRad).
For the gradient protocol, the microsomal pellet was resuspended in TNE buffer (25 mM TrisHCl pH 7.5, 150 mM NaCl, 5 mM EDTA) according to Borner et al.. The microsomal fraction (6 mg protein equivalent) was treated with 1% (w/w) Triton X-100 for 30 min at 4°C under gentle shaking. After solubilization, membranes were brought to a 1.8 M sucrose final concentration (using a 2.4 M sucrose/TNE buffer), overlaid with 2 ml of 1.6 M, 1.4 M, 1.2 M and 0.15 M sucrose in TNE buffer, and then spun for 16 h at 200.000 g at 4°C. DIMs were collected at the 1.2-1.4 M sucrose interface, washed with an excess of TNE buffer and centrifuged at 100.000 g for 1 hour to remove residual sucrose. The DIM pellet (G-DIMs) was homogenized in 1 ml of beta-octylglucoside containing buffer (60 mM beta-octylglucoside, 10 mM Tris HCl pH7.5, 150 mM NaCl). Protein concentration was determined with the RCDC Bio-Rad protein assay.
Free sterol extraction and analysis
Total lipids were extracted from microsomal and DIM fractions (R and G; 100 μg equivalent proteins per sample, previously diluted in 0.37 M KCl to reach a final volume of 500 μl) according to Folch et al. with chloroform/methanol (2:1, v/v). The extraction of lipids was carried out in 25 ml glass tubes with Teflon lined screw caps. Sterol internal standard (10 μg epichoprostanol) was added to Mic, R and G samples but not into one Mic sample (Mic-Std). All solutions were then mixed with MetOH/chloroform 2:1 (v/v), shaken and left overnight at 4°C. The next day, chloroform was added to reach a final ratio MetOH/chloroform 2:4 (v/v) and the mixture was centrifuged at 320 g for 8 min. The sterol-containing lower phase was collected, dried under nitrogen flux, washed once with EtOH and dried again. Lipids were saponified using ethanolic KOH (1 ml EtOH, 100 μl KOH (11 N)). Upon warming at 80°C for 1 h, 2 ml h20 and 2 ml hexane were added and the lipids were recovered by centrifugation (320 g, 8 min). The upper phase was evaporated to dryness under nitrogen flux. Then, the residue was derivatized by adding 100 μl of the silylating agents (BSTFA/TMCS mixture (5:1 v/v)) and warmed at 80°C for 1 hour. Samples were finally analyzed for their sterol content by gas chromatography after addition of 400 μl hexane.
Gas chromatography analyses were carried out on Agilent 7890 GC instrument equipped with a with a flame ionization detector, on a Varian Factor Four VF-5 Ms capillary column (15 M, 0.32 mM i.d. × 0.25 μm film thickness). Sample manual injection (1 μl) was performed in splitless mode (split vent at 30’seconds; injector temperature 240°C). Helium was the carrier gas at 1.5 ml/min in constant flow mode. Temperature program was programmed from 120°C to 240°C at 9°C/min. Data were processed using the Agilent EZ Chrom Elite software providing retention time and area for each compound of interest.
Quantification of sphingolipid long-chain bases
LCB content of DIM and microsomal fractions were determined as previously described Cacas et al. from three individual experiments. Briefly, LCB were released from fractions by direct overnight incubation at 110°C in 1 ml dioxane and 1 ml 10% (w/v) Ba(OH)2 solution prepared in water. Before incubation, standard LCB (d14:1, d17:1 and d20:0) used for quantification were directly added to the dioaxane/barium mixture (10 μg for each standard LCB/sample). Upon cooling and addition of 6 ml distilled water, LCB were extracted twice with 4 ml diethylether. Pooled diethylether phases were dried under nitrogen flux. Dry residues were dissolved in 1 ml methanol containing 100 μl of a freshly prepared 0.2 M metaperiodate (NaIO4) solution. Oxidation of extracted LCB into aldehydes was then carried out in the dark for 1 hour at room temperature and under mild shaking, as described by Kojima et al.. LCB-derived long-chain aldehydes were extracted into 1 ml hexane following addition of 1 ml water. To concentrate samples, the aldehyde-containing hexane phase was dried under nitrogen flux, and aldehydes were finally resuspended in 100 μl hexane to be injected into GC-MS.
For the separation of LCB-derived fatty aldehydes, a 30 M × 250 μm HP-5MS capillary column (5% phenyl-methyl-siloxane, 0.25 μm film thickness, Agilent) was used with helium carrier gas at 2 ml/min; injection was in splitless mode; injector and MS detector temperatures were set to 250°C (Agilent 6850 coupled to a mass analyzer Agilent 6975); the oven temperature was held at 50°C for 1 min, then programmed with a 25°C/min ramp to 150°C (2 min hold), a 10°C/min ramp to 210°C, and 75°C/min ramp to 320°C (5 min hold). Upon separation by GC and detection by MS, fatty aldehydes were identified based on their retention time and fragmentation . The ion current of each molecular species of interest was determined and further used for calculating the amount of molecules by comparison with the appropriate internal standards. These results were expressed in micrograms. Taking into account the molecular weight of individual LCB-derived aldehydes, the quantity in moles for each molecular species was calculated and expressed as mole%.
One-dimensional SDS-PAGE and nano-LC-MS/MS analysis
Microsomal and DIMs samples (20 μg protein equivalent) were mixed at a ratio of 1 to 1 with Laemmli buffer  without any heating denaturation step. Samples were separated onto small 12% polyacrylamide gels with 4.5% stacking gel and proteins were stained with colloidal blue (G250). Proteins were separated along a short (1°Cm)-migration. Individual gel lanes were sliced in 7 pieces for in-gel digestion and LC-MS/MS analysis. Each section was washed in water and completely destained using 100 mM NH4CO3 in 50% acetonitrile (ACN). A reduction step was performed by addition of 100 μl of 50 mM NH4CO3, pH 8.9, and 10 μl of 10 μm TCEP (Tris(2-carboxyethyl) phosphine HCl) at 37°C for 30 min. The proteins were alkylated by adding 100 μl of 50 mM iodoacetamide and allowed to react in the dark at 20°C for 40 min. Gel sections were first washed in water, then ACN, and finally dried for 30 min. In-gel digestions were performed with trypsin in the Progest system (Genomic Solution, East Lyme, CT, USA) according to a standard protocol. Gel pieces were washed twice by successive baths of 10% (v/v) acetic acid, 40% (v/v) ethanol and ACN. They were then washed twice with successive baths of 25 mM NH4CO3 and ACN. Digestion was subsequently performed for 6 h at 37°C with 125 ng of modified trypsin (Promega) dissolved in 20% (v/v) methanol and 20 mM NH4CO3. Peptides were extracted successively with 2% (v/v) TFA and 50% (v/v) ACN and then with pure ACN. Peptide extracts were dried and suspended in 20 μl of 0.05% (v/v) TFA, 0.05% (v/v) HCOOH, and 2% (v/v) ACN.
Mass spectrometry analysis was carried out on 2 independent replicates for each DIM fraction (R and G). Peptide separation was performed using an Eksigent 2D-ultra-nanoLC (Eksigent Technologies, Livermore, CA, USA) equipped with a C18 column (5 μm, 15°Cm × 75 μm, PepMap, LC packing). The mobile phase consisted of a gradient of solvents A 0.1% HCOOH (v/v) in water and B 99.9% ACN (v/v), 0.1% HCOOH (v/v) in water. Peptides were separated at a flow rate of 0.3 μl/min using a linear gradient of solvent B from 5 to 30% in 60 min, followed by an increase to 95% in 10 min. Eluted peptides were online analysed with a LTQ XL ion trap (Thermo Electron) using a nanoelectrospray interface. Ionization (1.5 kV ionization potential) was performed with a liquid junction and a non-coated capillary probe (10 μm i.d.; New Objective). Peptide ions were analyzed using Xcalibur 2.0.7, with the following data-dependent acquisition steps  full MS scan (mass to charge ratio (m/z) 300-2000, centroid mode), (2) MS/MS (qz = 0.25, activation time = 30 Ms, and collision energy = 35%, centroid mode). Step 2 was repeated for the three major ions detected in step 1. Dynamic exclusion was set to 45’s.
Protein identification and quantification
Searches were performed using the Mascot search engine (http://www.matrixscience.com) on the Medicago truncatula pseudomolecule database (http://www.jcvi.org/cgi-bin/medicago/annotation.cgi) version 3.5v3 (47529 entries). Trypsin digest was set to enzymatic cleavage, carboxyamidomethylation of, and oxidation of methionines were defined as fixed and variable modifications, respectively. Precursor mass precision was set to 2.0 Da with a fragment mass tolerance of 0.5 Da. Sequences corresponding to keratins or trypsin were removed by querying a homemade contaminant database as a first step of filtration. Identified proteins were validated according to the presence of at least two peptides with an E value smaller than 0.05. To take redundancy into account, proteins were grouped according to the Legoo server (http://www.legoo.org/).
Quantification of proteomic data was achieved by normalized spectral abundance factor (NSAF) analysis . As NSAF represent percentages, all data were arsin square root-transformed to obtain a distribution of values that could be checked for normality  by using the Kolmogorov-Smirnov test at a 95% confidence interval. The protocol effect on protein abundance was analyzed by the Student’s t-test using the XLSTAT software package.
In silico predictions
Alpha-helical TM spans and signal peptides were predicted according to the Phobius algorithm (http://phobius.sbc.su.se), whereas the online tool (http://biophysics.biol.uoa.gr/PRED-TMBB/input.jsp) was employed to discriminate trans-membrane beta-strand protein domains. N-myristoylation, S-palmitoylation and GPI anchor predictions were inferred from (http://mendel.imp.ac.at/myristate/SUPLpredictor.htm), (http://csspalm.biocuckoo.org/online.php), and (http://gpi.unibe.ch/), respectively. Close homologues of the identified proteins in Medicago were searched against The Arabidopsis Information Resource  (http://www.arabidopsis.org/) database with at least 70% pair-wise identity and a cut-off expectation value of e40. When necessary, ChloroP (http://www.cbs.dtu.dk/services/ChloroP/) was used to predict cTP, and homologies were searched within the HAMAP database (http://www.pdg.cnb.uam.es/cursos/Leon_2003/pages/visualizacion/programas_manuales/spdbv_userguide/us.expasy.org/sprot/hamap/families.html) that takes stock of plastid genome-encoded proteins.
Availability of supporting data
The data sets supporting the results of this article are included within the article and its additional files.
We acknowledge financial support by the French ANR (Agence Nationale de la Recherche) TRANSMUT ANR-10-BLAN-1604-0, the Germaine de Stael program (TRANSBIO 26510SG), the Burgundy Regional Council (PARI Agrale 8), the French ANR PANACEA-ANR-NT09_517917 and the Région Aquitaine. We thank the platform Métabolome-Lipidome- of Bordeaux (http://www.biomemb.cnrs.fr/page_8ENG.html; https://www.bordeaux.inra.fr/umr619/RMN_index.htm) funded by the French program Infrastructure de Recherche, contract MetaboHUB-ANR-11-INBS-0010 for contribution to equipment. We are grateful to Benoît Valot from the PAPPSO platform (Gif/Yvette, France) for having performed mass spectrometry analysis.
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