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Table 1 Main literature background to microdomain preparations as related to initial fractions

From: Direct purification of detergent-insoluble membranes from Medicago truncatularoot microsomes: comparison between floatation and sedimentation

Organism

Organ/Culture

1st fraction

2nd fraction

Detergent

DIM recovery process

References

Tobacco

Leaves

Microsomes

PM

Triton X-100

F

[12]

Tobacco

Leaves

Microsomes

PM

Triton X-100

F

[5]

Arabidopsis

Root callus cultures

Microsomes

None

Triton X-100

F

[[20]]

Tobacco

Cell cultures

Microsomes

PM

Triton X-100

F

[6]

Tobacco

Cell cultures

Microsomes

PM

Triton X-100

F

[7]

Leek and Arabidopsis

Seedlings

Microsomes

GA, ER, PM

Triton X-100

F

[16]

Medicago

Roots

Microsomes

PM

Triton X-100

F

[13]

Human

Cell cultures

Cell pellets

None

Triton X-100

S

[[25]]

Human

Cell cultures

Mitochondria

Mmito

Triton X-114

S

[17]

Arabidopsis

Cell cultures

Microsomes

PM

Triton X-100

F

[14]

Oat and Rye

Leaves

Microsomes

PM

Triton X-100

F

[15]

Red beet

Roots

Vacuoles

Tonoplast

Triton X-100

F

[18]

Arabidopsis

Cell cultures

Vacuoles

Tonoplast

Triton X-100

F

[19]

  1. Organelle versus microsomes and DIM recovery processes: floatation on sucrose gradient (F) versus sedimentation (S). GA, ER, Mmito and PM, and refer to Golgi apparatus, endoplamic reticulum, mitochondrial membrane and plasma membrane, respectively. Bold characters highlight the two protocols used in the current study.