Figure 3

Comparison of the proteins identified in R- and G-DIMs relative the initial microsomal (Mic) fraction of M. truncatula roots. (A) One dimensional profile of the proteins (15 μg per lane) recovered in R (R) and G-DIMs (G) using variable Triton X-100 concentrations: 3:1, 3:2, 6:1 and 6:2 (detergent/protein ratio: final detergent concentration). (B) Venn diagram distribution of the 874 non redundant proteins overall identified using GeLC-MS/MS in the microsomal, R- and G-DIM fractions. (C) List of the proteins that display a differential accumulation (p < 0.05) between R- and G-DIMs. Comparison of protein abundance was performed using the Student’s t-test on arsin square root-transformed normalized spectral abundance factors (NSAF). NSAF ratios of proteins between the two DIM fractions are provided in column 2. (D) Venn diagram distribution of the 227 proteins that reproducibly display at least a 2-fold higher abundance in DIM fractions than in microsomes. Subsets termed “R2xspecific” and “G2xspecific” refer to the proteins uniquely enriched in R-and G-DIMs, respectively, whereas “RG2xcore” designates the proteins enriched in both R- and G-DIMs, relative to microsomes. (E) Representation of previously published plant DIM-associated proteins within the proteins enriched in R- and G-DIMs relative to microsomes, by using identification mapping tools and homology search. Bold characters refer to canonical plant DIM markers.