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Figure 2 | BMC Plant Biology

Figure 2

From: Direct purification of detergent-insoluble membranes from Medicago truncatularoot microsomes: comparison between floatation and sedimentation

Figure 2

Comparison of sterol and long-chain base (LCB) contents in R-DIMs(R) and G-DIMs (G) relative to the initial microsomal (Mic) fraction of M. truncatula roots. All experiments were performed with 100 μg protein equivalents (A) Representative GC profile of total extracted sterols in the three fractions. Epichoprostanol (O) was used as an internal standard (10 μg) to quantify major sterol peaks (*) and added to the Mic, R and G fractions but not to the Mic minus Std (Mic-Std) sample. (B) Sterol enrichment from 100 μg protein equivalents. Results are expressed as the means ± SE (vertical bars) of at least three independent preparations. (C) Representative distribution of LCB in the three fractions; Abbreviations used: t18:1(8Z): 4-hydroxysphing-8(Z)-enine, t18:1(8E): 4-hydroxysphing-8(E)-enine, t18:0: 4-hydroxysphinganine (phytosphingosine), d18:1(8Z): sphing-8(Z)-enine, d18:1(8E): sphing-8(E)-enine, d18:0: sphinganine (dihydrosphingosine), d18:2(4E,8E,Z): sphinga-4(E),8(E,Z)-dienine.

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