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Weighted gene coexpression correlation network analysis reveals a potential molecular regulatory mechanism of anthocyanin accumulation under different storage temperatures in ‘Friar’ plum

BMC Plant Biology volume 21, Article number: 576 (2021) Cite this article

Abstract

Background

Flesh is prone to accumulate more anthocyanin in postharvest ‘Friar’ plum (Prunus salicina Lindl.) fruit stored at an intermediate temperature. However, little is known about the molecular mechanism of anthocyanin accumulation regulated by storage temperature in postharvest plum fruit.

Results

To reveal the potential molecular regulation mechanism of anthocyanin accumulation in postharvest ‘Friar’ plum fruit stored at different temperatures (0 °C, 10 °C and 25 °C), the fruit quality, metabolite profile and transcriptome of its flesh were investigated. Compared to the plum fruit stored at 0 °C and 25 °C, the fruit stored at 10 °C showed lower fruit firmness after 14 days and reduced the soluble solids content after 21 days of storage. The metabolite analysis indicated that the fruit stored at 10 °C had higher contents of anthocyanins (pelargonidin-3-O-glucoside, cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and quercetin-3-O-rutinose), quercetin and sucrose in the flesh. According to the results of weighted gene coexpression correlation network analysis (WGCNA), the turquoise module was positively correlated with the content of anthocyanin components, and flavanone 3-hydroxylase (F3H) and chalcone synthase (CHS) were considered hub genes. Moreover, MYB family transcription factor APL (APL), MYB10 transcription factor (MYB10), ethylene-responsive transcription factor WIN1 (WIN1), basic leucine zipper 43-like (bZIP43) and transcription factor bHLH111-like isoform X2 (bHLH111) were closely related to these hub genes. Further qRT–PCR analysis verified that these transcription factors were specifically more highly expressed in plum flesh stored at 10 °C, and their expression profiles were significantly positively correlated with the structural genes of anthocyanin synthesis as well as the content of anthocyanin components. In addition, the sucrose biosynthesis-associated gene sucrose synthase (SS) was upregulated at 10 °C, which was also closely related to the anthocyanin content of plum fruit stored at 10 °C.

Conclusions

The present results suggest that the transcription factors APL, MYB10, WIN1, bZIP43 and bHLH111 may participate in the accumulation of anthocyanin in ‘Friar’ plum flesh during intermediate storage temperatures by regulating the expression of anthocyanin biosynthetic structural genes. In addition, the SS gene may play a role in anthocyanin accumulation in plum flesh by regulating sucrose biosynthesis.

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Background

Anthocyanin is a kind of water-soluble flavonoid that is derived from the branch of flavonoids, and it gives flowers and fruits various and graceful colours [1, 2]. As an antioxidant, anthocyanin can effectively remove free radicals such as reactive oxygen species (ROS) when plants suffer environmental stress, protecting plants from damage [3]. In addition, it has been shown that anthocyanin intake is beneficial to human prevention of cardiovascular diseases and cancer [4]; thus, anthocyanin has been widely studied recently.

The biosynthetic pathway of anthocyanins in higher plants is conserved, and anthocyanins are synthesized from phenylalanine catalysed by a series of enzymes. The enzyme-associated genes involved in anthocyanin synthesis are divided into early biosynthesis genes (EBGs) and late biosynthesis genes (LBGs) [5]. EBGs include chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H) and flavonoid-3′-hydroxylase (F3’H), and they are common to different flavonoid synthesis branches [6, 7]. LBGs mainly include dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase/anthocyanin synthetase (LDOX/ANS) and UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT), and they contribute to the production of various anthocyanin components by catalysing flavanonol and its subsequent derivatives [8]. Anthocyanin biosynthesis-related genes are regulated by many transcription factors, among which MYB-bHLH-WD40 (MBW) has been widely studied. The MBW complex positively regulates the expression of structural genes by binding to cis-acting elements on the promoter regions of genes (such as DFR, LDOX/ANS, UFGT, etc.) and then facilitates the accumulation of anthocyanin in plants [7, 9,10,11,12]. In addition, transcription factors such as COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1), JAZ (JASMONATE ZIM-DOMAIN), NAC (NAM, ATAF1/2, CUC2), SPL (SQUAMOSA promoter-binding protein-like) and WRKY have been considered to regulate anthocyanin biosynthesis by interacting with the MBW complex [13,14,15,16,17,18].

Anthocyanin accumulation can be affected by light, temperature, hormones and mineral nutrition, and favourable low-temperature conditions are one of the important factors that induce the biosynthesis of anthocyanins [16, 19,20,21,22,23,24]. In grape fruit, cold treatment (4 °C) enhanced the expression levels of VvF3H, VvPAL, VvCHS3, VvCHS2 and VvLDOX in the peel and subsequently led to anthocyanin accumulation [25]. Blood orange stored at 4 °C exhibited 3 times higher anthocyanin content in the flesh than that stored at 25 °C [20]. Compared with the 27 °C treatment, the apple fruit under a lower temperature (17 °C) showed a higher anthocyanin content and reddened quickly. Further study showed that the transcription factor MdbHLH3 played a crucial role by interacting with MdMYB1 to upregulate the expression of MdDFR and MdUFGT by binding their promotor regions [23]. In peach, the anthocyanin content in the flesh increased significantly when the fruit was stored at 16 °C, while the transcript levels of anthocyanin biosynthesis-associated genes were enhanced [26]. The flesh of ‘Friar’ plum fruit is prone to reddening when it is stored at 5–15 °C, and it was found that the main anthocyanin component is cyanidin-3-O-glucoside [27, 28]. However, the molecular regulation mechanism of the intermediate temperature causing flesh reddening in ‘Friar’ plum fruit is less well known. In the present study, flesh colouration in postharvest ‘Friar’ plum fruit stored at different temperatures (0 °C, 10 °C and 25 °C) was observed, and subsequently, metabolite profile and comparative transcriptome analysis in the flesh was performed. By constructing a coexpression network through WGCNA, hub genes and candidate regulatory transcription factors were identified. These findings provide new insights into the mechanism of intermediate temperature-induced anthocyanin accumulation in postharvest plum fruit.

Results

Effect of different storage temperatures on the fruit quality of ‘Friar’ plum

Within 28 days of storage, there were no obvious colour changes in the flesh of fruit stored at 0 °C and 25 °C, while the flesh of fruit stored at 10 °C turned red on the 14th day of storage (Fig. 1a). Among the three different storage temperatures, the firmness of the fruit stored at 10 °C decreased the fastest, while the fruit stored at 0 °C decreased the slowest (Fig. 1b). After 21 days of storage, the soluble solids content (SSC) in the fruit stored at 10 °C appeared significantly lower than that in the fruit stored at 0 °C and 25 °C (Fig. 1c).

Fig. 1
figure 1

Quality changes of ‘Friar’ plum fruit under different storage temperatures. Flesh colour (a), firmness (b), and soluble solids content (SSC) (c). Different lowercase letters above the bars indicate significant differences (P < 0.05), which were obtained based on one-way ANOVA by LSD and DUNCAN tests

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Identification of the anthocyanin and phenolic metabolite profile related to flesh reddening

The UPLC–MS/MS results showed that the anthocyanins in the flesh of ‘Friar’ plum fruit mainly contained pelargonidin 3-O-glucoside, cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, and quercetin-3-O-rutinose, and their contents were much higher in fruit stored at 10 °C after 21 days than in those stored at 0 °C and 25 °C (Fig. 2a-d). Moreover, the contents of cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were much higher than pelargonidin 3-O-glucoside and quercetin-3-O-rutinose, indicating that cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were the main ingredients contributing to flesh reddening in ‘Friar’ plum fruit. In this work, polyphenolic components were also studied. Only the quercetin content in the flesh of fruit stored at 10 °C accumulated markedly, and the other polyphenolic components, such as neochlorogenic acid, chlorogenic acid, epicatechin, catechin hydrate, and quinic acid, were ubiquitous and less changed among all storage conditions (Fig. 2e-j), suggesting that quercetin was also related to the process of flesh reddening in ‘Friar’ plum fruit.

Fig. 2
figure 2

Changes in anthocyanin and polyphenolic components in ‘Friar’ plum flesh under different storage temperatures. a Pelargonidin-3-O-glucoside, b Cyanidin-3-O-glucoside, c cyanidin-3-O-rutinoside, d Quercetin-3-O-rutinose, e Quercetin, f Neochlorogenic acid, g Chlorogenic acid, h Epicatechin, i Catechin acid, and j Quinic acid. Different lowercase letters above the bars indicate significant differences (P < 0.05), which were obtained based on one-way ANOVA by LSD and DUNCAN tests. Nd stands for non-detectable

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Transcriptome analysis

To explore the regulatory mechanism of anthocyanin accumulation in the flesh of ‘Friar’ plum fruit, the samples at five detection time points (0, 7, 14, 21, 28 d) from the three different storage temperatures were used for deep RNA-seq analysis. After sequencing quality control, a total of 307.14 Gb of clean data were obtained, and the Q30 base percentage of each sample was not less than 91.47%. Then, the clean data were mapped to the European plum reference genome, with the mapping ratio varying from 84.98 to 93.00%. A total of 129,821 annotated genes were obtained. Differentially expressed genes (DEGs) were identified based on their expression levels in different samples, and functional annotation and enrichment analysis were performed. A total of 40,417 genes were differentially expressed under storage temperatures of 10 °C, 25 °C, and 0 °C (Fig. 3a). To screen the candidate genes related to anthocyanin biosynthesis, our study mainly focused on the DEGs at 10 °C vs. 0 °C and 10 °C vs. 25 °C. There were 2807, 4008, 4450, and 4234 genes that were differentially expressed on Days 7, 14, 21, and 28, respectively, at 10 °C vs. 0 °C and 10 °C vs. 25 °C (Fig. 3b). Among them, 467 genes were consistently differentially expressed (Fig. 3b).

Fig. 3
figure 3

Summary of differentially expressed genes under different storage temperatures in ‘Friar’ plum. a Number of DEGs in different DEG sets. T0–7 vs. T10–7 represents the DEG set in which samples stored at 0 °C for 7 days versus samples stored at 10 °C for 7 days. b Venn diagram shows DEGs in both 10 °C vs. 0 °C and 10 °C vs. 25 °C at different storage time points. DAT represents days after treatment

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Construction of a WGCNA and coexpression network

To obtain hub genes related to anthocyanin accumulation, the relationships of DEGs, anthocyanin components and storage temperature for each sample were analysed by constructing a WGCNA (Fig. 4). Sample clustering showed that the three biological replicates of each treatment were very good (Fig. 4a). Ten coexpression modules were identified by WGCNA (Fig. 4b), among which the turquoise module was positively correlated with the contents of pelargonidin-3-O-glucoside (r = 0.67, p value = 3e-06), cyanidin-3-O-glucoside (r = 0.80, p value = 8e-11), cyanidin-3-O-rutinoside (r = 0.75, p value = 4e-08), and quercetin-3-O-rutinose (r = 0.83, p value = 6e-11). In addition, the turquoise module was positively correlated with the storage temperature of 10 °C, and the correlation coefficient was 0.90 (p value = 6e-15) (Fig. 4c). According to GO and KEGG enrichment of the candidate genes in the turquoise module (1416 genes in total), 33 genes were mapped to the flavonoid metabolism pathway, and 31 genes were mapped to the starch and sugar metabolism pathway (see Additional file 1: Figs. S1 and S2).

Fig. 4
figure 4

Weighted gene coexpression network analysis of ‘Friar’ plum fruit under different storage temperatures. a Sample clustering. A01–03 (initial sample); B01–03, C01–03, D01–03 (sample stored at 0 °C, 10 °C, and 25 °C for 7 days, respectively); B04–06, C04–06, D04–06 (sample stored at 0 °C, 10 °C, and 25 °C for 14 days), B07–09, C07–09, D07–09 (sample stored at 0 °C, 10 °C, and 25 °C for 21 days), B10–12, C10–12, D10–12 (sample stored at 0 °C, 10 °C, and 25 °C for 28 days). b Hierarchical clustering showing modules of coexpressed genes. c Module/trait correlations and corresponding p values. The right panel shows a colour scale for module/trait correlations from −1 to 1

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Identification of candidate genes involved in anthocyanin biosynthesis

A total of 43 structural genes involved in anthocyanin biosynthesis were obtained in the turquoise module, and a heatmap of their expression profiles in the flesh of ‘Friar’ plum fruit was drawn based on their FPKM value (log10(FPKM+1)) (Fig. 5). The 43 structural genes from all major steps of the anthocyanin biosynthesis pathway were distributed as follows: four phenylalanine ammonia-lyase genes (PAL), one 4-coumarate: coenzyme A ligase (4CL), fourteen chalcone synthase genes (CHS), four chalcone isomerase genes (CHI), one flavonoid-3′-hydroxylase gene (F3’H), four flavanone 3-hydroxylase genes (F3H), three dihydroflavonol 4-reductase genes (DFR), five leucoanthocyanidin dioxygenase/anthocyanin synthase genes (LDOX/ANS) and seven UDP-glucose: flavonoid 3-O-glucosyltransferase genes (UFGT).

Fig. 5
figure 5

Analysis of genes related to anthocyanin biosynthesis in the turquoise module. a Anthocyanin biosynthetic pathway. The bold font indicates the genes obtained in the turquoise module and the anthocyanin components detected in the flesh of ‘Friar’ plum fruit. b Heatmap of the expression levels of differentially expressed genes (DEGs) involved in anthocyanin biosynthesis. c qRT–PCR detection of anthocyanin synthesis-related structural genes, PsPAL (Pd.00 g835470), PsCHS (Pd.00 g300780), PsCHI (Pd.00 g402750), PsF3H (Pd.00 g891590), PsF3’H (Pd.00 g637710), PsDFR (Pd.00 g1089860), PsLDOX (Pd.00 g746630), and PsUFGT (Pd.00 g247850). d Correlation analysis of the expression profiles in qRT–PCR (qPCR) and transcriptome data (RNA-seq) (N = 13, |r| > 0.55 represents a significant correlation between the two sets of data)

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Furthermore, the expression patterns of eight representative structural genes involved in anthocyanin biosynthesis, PsPAL (Pd.00 g835470), PsCHS (Pd.00 g300780), PsCHI (Pd.00 g402750), PsF3H (Pd.00 g891590), PsF3’H (Pd.00 g637710), PsDFR (Pd.00 g1089860), PsLDOX (Pd.00 g746630) and PsUFGT (Pd.00 g247850), were studied via qRT–PCR, and the transcripts of these genes were significantly higher in the flesh of plum fruit stored at 10 °C than in that stored at 0 °C and 25 °C (Fig. 5c), which was consistent with the results of transcriptome analysis based on the correlation analysis (Fig. 5d).

Identification of genes involved in carbohydrate metabolism

Carbohydrates are considered substrates for anthocyanin synthesis, and the change in soluble sugar content was detected by HPLC. The contents of glucose, fructose and sorbitol showed downward trends at three different storage temperatures. The sucrose content decreased in the flesh of plum fruit during 0 °C storage but increased during 10 °C and 25 °C storage; moreover, it was higher at 0 °C than at 25 °C (Fig. 6a). In the turquoise module, which was related to anthocyanin synthesis, five genes were involved in starch and sugar metabolism, including two hexokinases (HXKs) and three sucrose synthases (SSs) (Fig. 6b). Correlation analysis showed that the expression patterns of the SS genes were positively correlated with sucrose content and anthocyanin content (Fig. 6c), suggesting that higher expression levels of these genes were beneficial to carbohydrate metabolism, which contributed to anthocyanin accumulation in the flesh under storage at 10 °C.

Fig. 6
figure 6

Soluble sugar content and expression pattern of carbohydrate metabolism-related genes under different storage temperatures. a Glucose, fructose, sorbitol and sucrose contents in ‘Friar’ plum fruit under different storage temperatures. b Heatmap of the expression levels of carbohydrate metabolism-related genes in the turquoise module. c Correlation analysis of anthocyanin content, soluble sugar content and expression profiles of carbohydrate metabolism-related genes (N = 13, |r| > 0.55 represents a significant correlation between the two sets of data). Different lowercase letters above the bars indicate significant differences (P < 0.05), which were obtained based on one-way ANOVA by LSD and DUNCAN tests

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Screening potential transcription factors that regulate anthocyanin synthesis

To further explore the molecular regulatory mechanism of anthocyanin biosynthesis in the flesh of ‘Friar’ plum fruit, a coexpression network was constructed based on the genes present in the turquoise module. In the network, three F3H genes (Pd.00 g799840, Pd.00 g891590 and Pd.00 g617550) and two CHS genes (Pd.00 g276460 and Pd.00 g113960) were identified as hub genes (Fig. 7a), and five transcription factor genes, MYB10, APL, WIN1, bHLH111 and bZIP43, were found to be coexpressed with anthocyanin biosynthesis-related genes. Among the five transcription factor genes, MYB10 and WIN1 were closely related to CHS, F3H, DFR and LDOX, APL was closely related to CHS, F3H and DFR, bHLH111 was only closely related to F3H, and bZIP43 was closely related to CHS and F3H. In addition, there was a positive correlation between APL and WIN1 (Fig. 7b).

Fig. 7
figure 7

Coexpression network analysis of the potential key genes in turquoise modules based on WGCNA. a Coexpression network of the genes (weight > 0.5). b Coexpression network of transcription factors and anthocyanin biosynthesis-related structural genes. c Heatmap of the expression levels of the candidate transcription factors. d qRT–PCR detection of candidate transcription factor genes PsAPL (Pd.00 g301130), PsWIN1 (Pd.00 g307540), PsMYB10 (Pd.00 g623010), PsbHLH111 (Pd.00 g1033540), and PsbZIP43 (Pd.00 g297380). e Correlation analysis of the expression profiles in qRT–PCR (qPCR) and transcriptome data (RNA-seq) (N = 13, |r| > 0.55 represents a significant correlation between the two sets of data)

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The heatmap based on the transcriptome data showed that the expression levels of APL, WIN1, MYB10, bHLH111 and bZIP43 were obviously upregulated in the fruit stored at 10 °C, while there was little change in the fruit stored at 0 °C and 25 °C (Fig. 7c). Meanwhile, the qRT–PCR results indicated that the transcripts of these five detected genes in the fruit stored at 10 °C were markedly higher than those in the fruit stored at 0 °C and 25 °C (Fig. 7d), which was consistent with the transcriptome analysis results based on the correlation analysis (Fig. 7e). Furthermore, the expression patterns of the five transcription factor genes were positively correlated with the changes in the expression levels of the structural genes and the anthocyanin content (see Additional file 1: Fig. S3). It was proposed that APL, WIN1, MYB10, bHLH111 and bZIP43 might be involved in anthocyanin accumulation by regulating the expression of structural genes associated with anthocyanin biosynthesis.

Discussion

Intermediate temperature is beneficial to promote anthocyanin accumulation in plum fruit

Temperature is an important environmental factor that affects anthocyanin accumulation. Low temperature (4 °C) can significantly induce anthocyanin accumulation in Arabidopsis seedlings in the presence of light [24]. Intermediate temperature (16 °C) effectively leads to reddening in the leaves of apple and begonia [29, 30], and 15 °C treatment promotes anthocyanin accumulation in grape peel [31]. For postharvest fruits, intermediate temperature also promotes the process of anthocyanin accumulation in peach, kiwifruit, sweet orange and plum fruits [26,27,28, 32,33,34,35]. In this work, the flesh significantly accumulated anthocyanins and appeared red on the 14th day of storage when the fruit was stored at 10 °C, while the flesh of the fruit stored at 0 °C and 25 °C showed lower anthocyanin contents and did not redden within 28 days of storage (Fig. 1a). This was similar to the findings of a previous report [27], in which the colour of flesh rapidly changed to red once the ‘Friar’ plum fruit was stored at 5 °C and 15 °C but reddened slowly at 0 °C or did not turn red at 25 °C. It also suggested that intermediate temperature was prone to accumulate anthocyanin in the flesh of ‘Friar’ plum fruit.

Anthocyanidins are widely distributed among diverse plant species. These are flavonoids that are usually present in the form of six common anthocyanidins, pelargonidin, cyanidin, delphinidin, peonidin, petunidin and malvidin. Anthocyanidins are very unstable; they can integrate with monosaccharides and disaccharides by glycosylation and then form various stable water-soluble anthocyanins [36, 37]. In this work, four anthocyanin components were identified in the flesh of ‘Friar’ plum fruit, and moreover, their contents significantly increased as the flesh reddened in plum fruit (Fig. 1 a and Fig. 2 a-d). This result was consistent with previous findings in plum fruit [27], indicating that flesh reddening under 10 °C storage was the result of the accumulation of these four anthocyanin components. The upregulation of structural genes resulted in anthocyanin accumulation [23, 24, 34, 38]. To understand the regulation of anthocyanin biosynthesis in ‘Friar’ plum fruit under intermediate temperature storage, 43 differentially expressed structural genes were found based on the transcriptome data. All of the candidate genes (PAL, 4CL, CHS, CHI, F3’H, F3H, FLS, DFR, LDOX/ANS and UFGT) were completely mapped to the anthocyanin biosynthesis pathway and covered almost all of the key genes of this pathway (Fig. 5a). Moreover, the flesh of fruit stored at 10 °C showed rapid reddening and demonstrated higher expression levels of structural genes, which were positively correlated with the accumulation of quercetin and anthocyanin (Fig. 2 and Fig. 5 c and Fig. S3). Therefore, it was confirmed that intermediate temperature (10 °C) storage could promote the red colour development of flesh by increasing the transcripts of anthocyanin synthesis-related structural genes. Carbohydrates are an important substrate for anthocyanidin transformation into anthocyanins, and they have been considered a signal for regulating anthocyanin biosynthesis [39]. Galactose is the main substrate for anthocyanin synthesis in apple, and glucose can activate the activity of hexokinase (MdHXK1), which subsequently phosphorylates the MdbHLH3 transcription factor to regulate fruit reddening [40, 41]. In grapes, carbohydrates can induce the expression of F3H and then stimulate anthocyanin accumulation [42]. Among the detected carbohydrates in this study, only the sucrose content significantly increased under 10 °C storage once the flesh appeared to redden at Day 14 (Fig. 1a and Fig. 6); in addition, the sucrose synthase (SS) genes showed higher expression levels. Thus, sucrose might be involved in anthocyanin synthesis as a substrate or signal in ‘Friar’ plum fruit stored at 10 °C.

Transcription factors involved in anthocyanin accumulation in plum fruit

Anthocyanin biosynthesis can be regulated by various transcription factors, and the MBW complex, which regulates anthocyanin accumulation by triggering structural genes, is well studied [43,44,45,46]. MYB10 has been proven to be involved in anthocyanin synthesis by regulating DFR, LDOX and UFGT in apple, pear, nectarine, apricot, sweet cherry, strawberry and mangosteen [22, 47,48,49,50,51], and PsMYB10.1 can participate in anthocyanin accumulation by regulating PsANS, PsUFGT and PsGST in the pericarp of the postharvest ‘Akihime’ plum (Prunus salicina Lindl.) under 20 °C/light treatment [32]. In addition, transient overexpression of PaMYB10 increased anthocyanin content in the peel of apricot fruit [52]. Heterologous overexpression of PpMYB10.1/PpbHLH3 and PpMYB10.3/PpbHLH3 activated anthocyanin production in tobacco leaves by upregulating NtCHS, NtDFR and NtUFGT [18, 53]. Two bHLH transcription factors, MdbHLH3 and MdbHLH33, have been confirmed to regulate DFR to promote anthocyanin biosynthesis by interacting with MYB10 in apple [54], and MdbHLH3 could regulate low temperature-induced anthocyanin synthesis by binding to the LTR (low temperature response) element of the promotor of MdMYBPA1 [55]. Moreover, bHLH3 could directly regulate the expression of structural genes to facilitate anthocyanin accumulation [23, 49]. In the present study, both transcriptome analysis and qPCR quantitative detection indicated that the expression level of MYB10 significantly increased once anthocyanin accumulated in the flesh of ‘Friar’ plum fruit under 10 °C storage, and this was coordinated with the expression patterns of structural genes (Fig. 7). Thus, the transcription factor MYB10 was involved in regulating anthocyanin accumulation in ‘Friar’ plum fruit under intermediate temperature storage. Except for MYB10, the expression profiles of APL and bHLH111 were also found to be closely related to anthocyanin accumulation and changes in the structural genes, and moreover, the expression patterns of APL and bHLH111 were similar to MYB10, indicating that the transcription factors APL and bHLH111 were also involved in regulating anthocyanin synthesis in ‘Friar’ plum fruit. The proposed function of APL is that it participates in the response to phosphorus and nitrogen deficiency and promotes flowering in Arabidopsis thaliana [56, 57]. bHLH111 has been reported to determine the competence of the pericycle for lateral root initiation in Arabidopsis thaliana [58]. However, their roles in regulating anthocyanin accumulation in postharvest fruit are unclear and need to be studied further.

The ethylene-responsive transcription factor WIN1 has been reported to be involved in wax biosynthesis and defence responses in Arabidopsis thaliana [59, 60]. In this study, 10 °C storage induced higher expression of WIN1, which was positively correlated with anthocyanin accumulation and the expression patterns of structural genes (Fig. 7); thus, WIN1 participated in the regulation of anthocyanin biosynthesis. Previous studies have shown that MdEIL1 (EIN3-LIKE1) and MdERF1b (ETHYLENE RESPONSE FACTOR1b) promote anthocyanin synthesis by regulating transcription factors such as MdMYB1, MdMYB9 and MdMYB11 in apple [61, 62]. It was found that WIN1 was closely related to APL based on the analysis of the coexpression network in the present study, so it was hypothesized that WIN1 might regulate anthocyanin synthesis by interacting with APL. The transcription factor bZIP43 was found to be associated with somatic embryogenesis induction and involved in stress responses by interacting with bHLH109 in Arabidopsis [63]. Considering that anthocyanin accumulation is one of the responses to environmental stress in plants, the transcription factor bZIP43 probably plays a role in regulating anthocyanin synthesis in ‘Friar’ plum fruit under intermediate storage temperatures.

Conclusions

This study found that an intermediate storage temperature (10 °C) accelerated the process of anthocyanin accumulation in ‘Friar’ plum fruit. Cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside contributed to the red colour of flesh. Based on the transcriptome and metabolite profile and subsequent WGCNA, 43 anthocyanin biosynthesis genes and two carbohydrate metabolism-associated genes were identified. According to the gene coexpression network constructed by WGCNA, F3H and CHS were recognized as hub genes, and five transcription factors, APL, WIN1, MYB10, bZIP43 and bHLH111, were proposed to be involved in anthocyanin biosynthesis by regulating the structural genes.

Methods

Materials and treatments

‘Friar’ plum fruit was harvested at commercial maturity from Yi County, Baoding City, Hebei Province. The plum fruit (average single fruit weight 110 g ± 9.25 g; soluble solids content 10.4% ± 0.96% and firmness 77.94 N ± 6.44 N) without visible defects were divided into three groups and each group (approximately 450 plums) were averagely placed in three cardboard boxes (44.5 × 31.5 × 20.5 cm3), and then stored in the dark at 25 ± 1 °C (25 °C), 10 ± 0.5 °C (10 °C), and 0 ± 0.5 °C (0 °C) with 85–90% relative humidity. The quality assays were performed at 7 d intervals during storage, with three repetitions of each treatment with 5 fruits per repetition, and the flesh samples were quickly frozen in liquid nitrogen and stored at −80 °C until use.

Determination of fruit quality

Firmness at two equidistant points on the equatorial region of fruit with skin removal was determined using a GY-4 digital fruit hardness metre (TOP Instruments Co., Hangzhou, Zhejiang, China), and firmness was calculated and expressed in Newtons (N). The soluble solids content (SSC) was measured by a PAL-1 pocket digital refractometer (ATAGO CO., LTD., Tokyo, Japan).

Determination of soluble sugar content

Five grams of flesh powder ground in liquid nitrogen was weighed, and then 5 mL of 85% ethanol solution was added for ultrasonic extraction at 40 °C for 1 h, followed by centrifugation at 8000 g for 10 min. The supernatant was collected. The extraction was repeated once more, and the supernatant was combined. After filtration through a 0.45 μm PTFE microporous membrane, the combined supernatant was collected for sugar analysis by HPLC (LC-20AT, SHIMADZU CORP., Kyoto, Japan). The measurement conditions were as follows: chromatographic column: Bio–Rad Aminex HPX-87H, detector: differential RID, mobile phase: 5 mM sulfuric acid, flow rate: 0.3 mL/min, column temperature: 40 °C, injection volume: 10 μL.

Determination of phenolic acid and flavonoid contents

Phenolic acid extraction

Phenolic acid extracts were carried out according to Wang et al. [64] with minor modifications. Briefly, 2 g of freeze-dried powder was extracted with 10 mL of 80% methanol containing 0.5% hydrochloric acid. Afterwards, the mixture was ultrasonicated for 30 min and then centrifuged at 10000 × g at 4 °C for 10 min. Finally, the supernatant was collected. The extraction was repeated twice, and the combined supernatants were evaporated until almost dry at 50 °C under a gentle nitrogen stream. The resultant dry residues were redissolved in 5 mL of 50% (v/v) methanol/ultrapure water and filtered through a 0.22 μm PTFE membrane filter (Pall, MI, USA).

Flavonoid extraction

Flavonoid extracts were performed according to the method of Gao et al. [65] with some modifications. Two grams of the freeze-dried powder of plum flesh was extracted with 30 mL of 80% methanol in the dark for 24 h at −20 °C. Afterwards, the resultant mixture was centrifuged at 10000 × g at 4 °C for 10 min, and then the supernatant was immediately collected for analysis. The flavonoid contents were determined using UPLC–MS/MS after filtration through 0.22 μm PTFE membrane filters (Pall, MI, USA).

UPLC–MS/MS analysis

Phenolic and flavonoid analyses were performed as described previously by Gao et al. [65]. The samples were analysed by using an Acquity UPLC system (Waters, Milford, MA) with a triple quadrupole mass spectrometer (TQ-S, Waters Micromass, Manchester, UK). An acquity HSS C18 column (1.8 μm particle size; 2.1 × 150 mm; Waters, Milford, MA, USA) was used to perform chromatographic separation. The column and sample managers were maintained at 40 °C and 10 °C, respectively. The mobile phase used for the separation consisted of (A) 0.1% (v/v) formic acid in water and (B) 0.1% (v/v) formic acid in acetonitrile. Samples were eluted according to a linear gradient: 0.5–4.5 min, 5–30% B; 4.5–9.0 min, 30–90%; 9.0–10.0 min, 0.5% B, and a flow rate of 0.3 mL min−1.

The mass spectrometer was operated in both positive and negative ionization modes, depending on the structure and properties of the compounds. The parameters were as follows: capillary voltage, + 2.5 kV/−1.0 kV; source temperature, 150 °C; desolvation temperature, 500 °C; cone gas flow, 150 L h−1; and desolvation gas flow, 1000 L h−1. Detection was carried out in multiple reaction monitoring (MRM) mode. All analyte-dependent parameters were conducted according to our previously published study [65]. MassLynxTM 4.1 software (Waters) was used for data acquisition and processing. Quantitative determinations were performed using the standard curves generated from individual compounds in serial dilutions (1–500 ng mL−1).

Transcriptome sequencing and WGCNA

Total RNA was extracted from ‘Friar’ flesh samples stored at 0 °C, 10 °C and 25 °C for 7 days, 14 days, 21 days and 28 days (three biological repetitions), and RNA concentration and purity were measured using a NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg of RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample. Library quality was assessed with the 2100 Bioanalyzer system. After the library inspection was qualified, different libraries were pooled according to the target data volume and sequenced by the Illumina platform. The off-board data were filtered to obtain clean data, compared with the reference genome of European plum [66] to obtain mapped data, and evaluated for library quality, such as insert length tests and randomness tests. Then, structural-level analysis, such as alternative splicing analysis, new gene discovery and gene structure optimization, was carried out. The expression levels of differentially expressed genes, functional annotation and functional enrichment of differentially expressed genes were analysed. Finally, the transcriptome data and phenotypic data were analysed by WGCNA using R language.

RNA extraction and qPCR analysis

Total RNA from flesh was extracted by the CTAB method [67]. RNA (0.8 μg, OD260:OD280 between 1.80 and 2.0, OD260:OD230 > 1.5, no obvious degradation by electrophoresis) was used for reverse transcription by PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara Biomedicals, Dalian, China). The products were diluted 15 times with nuclease-free water and then subjected to real-time fluorescence quantitative PCR (qPCR) with a TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit (TaKaRa Biomedicals).

Quantitative real-time (qRT)-PCR assays were conducted using an Applied Biosystems 7500 Fast Real-Time PCR System. The reaction system was 20 μL, including 10 μL of 2X Green Premix Ex Taq II (Tli RNaseH Plus), 0.8 μL each of gene specific upstream primer and downstream primer, 0.4 μL of ROX Reference Dye II (50X), 2 μL of diluted cDNA, and 6 μL of nuclease-free water. The running program was set as follows: 30 s at 95 °C for one cycle, 5 s at 95 °C and 34 s at 60 °C for 40 cycles. PsACTIN7 was used as the internal reference. The primers used in this paper are listed in Additional file 1: Table S1. The relative expression levels of genes were calculated according to the 2-△△Ct method.

Statistical analysis

Each experiment was performed in three replicates. Experimental results were analysed using GraphPad Prism 8, Origin 2021, IBM SPSS Statistics 23, RStudio, and Cytoscape 3.7.1 software. Error bars denote standard deviations. Different lowercase letters above the bars indicate significant differences (P < 0.05), which were obtained based on one-way ANOVA by LSD and DUNCAN tests using IBM SPSS Statistics 23 software.

Availability of data and materials

The datasets supporting the conclusions of this article are included within the article (and its additional file(s).

Abbreviations

WGCNA:

Weighted gene co-expression correlation network analysis

ROS:

Reactive oxygen species

EBGs:

Early biosynthesis genes

LBGs:

Late biosynthesis genes

PAL:

Phenylalnine ammonialyase

CHS:

Chalcone synthase

CHI:

Chalcone isomerase

F3H:

Flavanone 3-hydroxylase

F3’H:

Flavonoid-3′-hydroxylase

DFR:

Dihydroflavonol 4-reductase

LDOX/ANS:

Leucoanthocyanidin dioxygenase/Anthocyanin synthetase

UFGT:

UDP-glucose: flavonoid 3-O-glucosyltransferase

MBW:

MYB-bHLH-WD40

COP1:

CONSTITUTIVE PHOTOMORPHOGENIC 1

JAZ:

JASMONATE ZIM-DOMAIN

NAC:

NAM, ATAF1/2, CUC2

SPL:

SQUAMOSA promoter-binding protein-like

HXK:

Hexokinase

SS:

sucrose synthases

APL:

MYB family transcription factor APL

MYB10:

MYB10 transcription factor

WIN1:

Ethylene-responsive transcription factor WIN1

bZIP43:

Basic leucine zipper 43-like

bHLH111:

Transcription factor bHLH111-like isoform X2

LTR:

Low temperature response

MdEIL1:

EIN3-LIKE1

MdERF1b:

ETHYLENE RESPONSE FACTOR1b

DAT:

Days after treatment

SSC:

Soluble solids content

FPKM:

Fragments per kilobase of exon model per million mapped fragments

DEGs:

Differentially expressed genes

UPLC–MS/MS:

ultra-performance liquid chromatography–tandem mass spectrometry

HPLC:

High performance liquid chromatography

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Acknowledgements

We would like to acknowledge all the teachers and students from the laboratory of the College of Life Science of Hebei Normal University and the Agriculture Institute of Biotechnology and Food Science of Hebei Academy of Agriculture and Forestry Sciences for their help.

Funding

This work was supported by the HAAFS Agriculture Science and Technology Innovation Project (2019-2-1), the National Natural Science Foundation of China (315018012) and the Young Talents Fund of Hebei province.

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  1. Xueling Li and Yudou Cheng are co-first authors.

Authors and Affiliations

  1. College of Life Science, Hebei Normal University, Shijiazhuang, Hebei Province, 050024, People’s Republic of China

    Xueling Li & Sujuan Cui

  2. Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, Hebei Province, 050051, People’s Republic of China

    Xueling Li, Yudou Cheng & Junfeng Guan

  3. Plant Genetic Engineering Center of Hebei Province, Shijiazhuang, Hebei Province, 050051, People’s Republic of China

    Xueling Li, Yudou Cheng & Junfeng Guan

  4. Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agricultural and Forestry Sciences, Beijing, People’s Republic of China

    Meng Wang

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Contributions

J.F.G and Y.D.C conceived the project and designed the experiments. X.L.L performed the experiment and write the manuscript. M.W performed the phenolic acid and flavonoid detection. S.J.C, J.F.G and Y.D.C guided the operation of the experiment and assisted with writing. The author(s) read and approved the final manuscript.

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Correspondence to Junfeng Guan.

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Supplementary Information

Additional file 1: Figure S1

. GO enrichment of the genes in turquoise module. Figure S2. KEGG enrichment of the genes in turquoise module. Figure S3. Correlation analysis of expression profiles of anthocyanin biosynthesis related genes, anthocyanin components and quercetin content. Table S1. The primers used in this study.

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Li, X., Cheng, Y., Wang, M. et al. Weighted gene coexpression correlation network analysis reveals a potential molecular regulatory mechanism of anthocyanin accumulation under different storage temperatures in ‘Friar’ plum. BMC Plant Biol 21, 576 (2021). https://doi.org/10.1186/s12870-021-03354-2

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