- Research article
- Open Access
The grapevine R2R3-type MYB transcription factor VdMYB1 positively regulates defense responses by activating the stilbene synthase gene 2 (VdSTS2)
BMC Plant Biology volume 19, Article number: 478 (2019)
Resveratrol is a naturally occurring plant stilbene that exhibits a wide range of valuable biological and pharmacological properties. Although the beneficial effects of trans-resveratrol to human health and plant protection against fungal pathogens and abiotic stresses are well-established, yet little is known about the molecular mechanisms regulating stilbene biosynthesis in plant defense progress.
Here, we cloned and identified the Chinese wild grape (Vitis davidii) R2R3-MYB transcription factor VdMYB1, which activates defense responses against invading pathogen. VdMYB1 transcripts were significantly upregulated after inoculation with the grapevine powdery mildew fungus Erysiphe necator (Schw.) Burr. Transient expression analysis using onion epidermal cells and Arabidopsis thaliana protoplasts showed that VdMYB1 was localized in the nucleus. Yeast one-hybrid assays revealed that VdMYB1 acts as a transcriptional activator. Grapevine leaves transiently overexpressing VdMYB1 showed a lower number of fungal conidiophores compared with wild-type leaves. Overexpression of VdMYB1 in grapevine leaves did not alter the expression of genes in salicylic acid- and jasmonate-dependent pathways, but affected the expression of stilbene synthase (STS) genes, key regulators of flavonoid metabolism. Results of electrophoretic mobility shift assays and in vivo transcriptional activation assays showed that VdMYB1 binds to the MYB binding site (MYBBS) in the STS2 gene promoter, thus activating STS2 transcription. In heterologous expression assays using tobacco leaves, VdMYB1 activated STS2 gene expression and increased the accumulation of resveratrol.
Our study showed that VdMYB1 activates STS2 gene expression to positively regulate defense responses, and increases the content of resveratrol in leaves.
Recognition of the invading pathogen is a key requirement for disease resistance. In plants recognition of specific pathogens activates chemical signaling, which trigger diverse cellular defense responses . Many of these responses transmit the signals from the cell membrane to the nucleus, where these signals increases the transcript levels of several defense-related genes , which encode pathogenesis-related (PR) proteins and enzymes that produce antimicrobial secondary metabolites . Thus, transcriptional regulation of plant defense-related genes play a critical role in defense responses and disease resistance.
Transcription factors (TFs) bind to specific promoters, and activate the expression of downstream genes. In Arabidopsis thaliana, plant defense responses involve the activation of more than 1500 TFs belonging to six major families, including ERF, TGA, WRKY, Whirly, NAC (NAM, ATAF, and CUC), and R2R3 MYB. The ERF family TFs bind to the GCC-box, which is found in the promoters of several PR genes . Genomic targets of TGA TFs include PR genes and the gene encoding glutathione S-transferase . WRKY TFs bind the W-box elements in NON-EXPRESSOR OF PR1 promoter and other PR gene promoters. Whirly TFs bind to the PB element in the PR-10a gene promoter . NAC TFs target the promoter of EARLY RESPONSIVE TO DEHYDRATION, and activate the expression of defense genes .
The MYB superfamily is the largest known family of plant TFs, containing approximately 198 members in Arabidopsis and 183 in rice (Oryza sativa) [7, 8]. According to the number of conserved SWI3, ADA2, N-CoR, and TFIIIB (SANT) domains, The MYB TFs are divided into three subfamilies: MYB-related TFs (one SANT domain), R2R3-type TFs (two SANT domains), and R1R2R3-type TFs (three SANT domains) . The MYB proteins exhibit diverse functions in plant development, biotic and abiotic stress responses, primary and secondary metabolism, hormone synthesis, and signal transduction . Most of the R2R3-type MYB TFs function in the response to biotic and abiotic stresses. In Arabidopsis, AtMYB2, AtMYB21, AtMYB24, AtMYB30, AtMYB96, AtMYB102, and AtMYB108 function in stresses included by cold, wounding, salinity, drought, and pathogen infection . Moreover, PnMYB134 in Populus nigra was found in response to biotrophic rust fungus, MYB134 overexpressing and silencing lines accumulated higher and lower amounts of flavan-3-ols, respectively, resulting in altered pathogen infection . In grapevine (Vitis spp.), R2R3-type MYB proteins play positive or negative roles in the production of enzymes involved in the biosynthesis of flavonoids and phenylpropanoids [11,12,13]. However, few documents have reported that the MYBs regulating stilbenes are involved in grapevine defense response.
Grapevine is the most economically important tree fruit crop in the world [14, 15]. The most commonly cultivated species of grapevine, Vitis vinifera, is highly susceptible to the fungal pathogen Erysiphe necator (Schw.) Burr. . The resulting powdery mildew (PM) disease reduces fruit yield and wine quality. Therefore, identification of genetic sources of resistance to PM will be useful for grapevine breeders . China is a major center of origin of Vitis species, and some Chinese wild Vitis species show strong resistance to major pathogen, including pathogens of cultivated Vitis species . For example, the Chinese wild grapevine (V. davidii) shows strong resistance to several fungal pathogens, including E. necator . Although several disease resistance genes have been cloned from the Chinese wild grapevine V. pseudoreticulata, disease resistance genes in V. davidii are yet to be examined.
To explore the function of MYB family TFs in defense responses, we identified and cloned the MYB1 gene from V. davidii (VdMYB1). We show that VdMYB1 transcripts respond to pathogen infection, and VdMYB1 TF regulates the defense response by activating the stilbene synthase 2 (STS2) gene, which encodes a key enzyme in the stilbenoids biosynthesis pathway. Thus, our findings suggest that VdMYB1 play a key role in the defense response of grapevine to invading pathogens.
Identification of VdMYB1
Full-length cDNA of VdMYB1 was obtained by rapid amplification of cDNA ends (RACE) -PCR (GenBank accession: MK188872). Alignment of the genomic DNA of VdMYB1 with the whole genome sequence of V. vinifera cultivar Pinot Noir showed that VdMYB1 is located on chromosome 7 (Fig. 1a). By aligning the genomic and cDNA sequences of VdMYB1, we found that VdMYB1 contains two introns and three exons (Fig. 1a). The VdMYB1 open reading frame (ORF) encodes a predicted protein of 272 amino acids, with a theoretical isoelectric point (pI) of 6.51 and a deduced molecular mass of 30.437 kDa. Analysis using SMART and PROSITE programs revealed an R2R3-type MYB domain at the N-terminus of VdMYB1 (Fig. 1a, b). Phylogenetic analysis showed that the R2R3-type MYB genes from related species clustered into two subgroups (Fig. 1c).
VdMYB1 expression is induced by various defense signals
To investigate whether VdMYB1 is involved in defense responses, we analyzed VdMYB1 transcript levels in detached leaves of V. davidii cv. Tangwei after inoculation with PM pathogen E. necator using quantitative real-time PCR (qRT-PCR). The level of VdMYB1 transcripts was significantly increased in response to PM infection, as soon as 8 h post-inoculation (hpi), reaching a peak at 16 hpi, and VdMYB1 expression was maintained at a high level until 24–48 hpi (Fig. 2a). To determine whether VdMYB1 also responds to plant hormones, V. davidii cv. Tangwei leaves were treated with SA, methyl jasmonate (MeJA), and the VdMYB1 expression level was investigated by qRT-PCR. Results showed that VdMYB1 transcripts can quickly response to SA, and the expression level reached the first peak at 24 h post treatment (hpt), and declined at 36 hpt, then surged to its second peak at 48 hpt. However, VdMYB1 transcripts showed no significant change under MeJA treatment (Fig. 2b).
VdMYB1 encodes a functional transcription factor
To study the subcellular localization of VdMYB1, we generated a construct encoding a fusion of VdMYB1 with the green fluorescent protein (GFP) under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Introduction of the construct into onion epidermal cells and Arabidopsis protoplasts revealed GFP signal in the nuclei (Fig. 3) By contrast, introduction of the GFP gene alone under the control of CaMV 35S promoter into onion epidermal cells showed fluorescence throughout the cell (Fig. 3a). In Arabidopsis protoplasts, the VdMYB1-GFP fusion protein was also localized to the nucleus (Fig. 3b). Together, these observations indicate that VdMYB1 localizes to the nucleus.
To test whether VdMYB1 functions as a transcriptional activator, we fused the VdMYB1 ORF to the GAL4 DNA binding domain in the pGBKT7 vector, and introduced the construct into yeast (Saccharomyces cerevisiae) strain AH109. Transformed yeast cells harboring the pCL construct (expressing full-length GAL4), pGBKT7 empty vector, or pGBKT7/VdMYB1 grew well on synthetic defined (SD) medium lacking tryptophan (SD/−Trp) (Fig. 3c). Yeast cells transformed with pGBKT7/VdMYB1 or pCL grew better on SD medium lacking Trp, adenine (−Ade), and histidine (−His) (SD/−Trp-Ade-His), and also showed β-galactosidase activity (Fig. 3c). Cells harboring the negative control plasmid pGBKT7 showed no growth on SD/−Trp-Ade-His medium and no β-galactosidase activity (Fig. 3c). These data suggest that the R2R3-type MYB protein VdMYB1 functions as a transcriptional activator in yeast.
Transient expression of VdMYB1 in grapevine affects defense responses
To examine the function of VdMYB1 in plant defense, VdMYB1 was transiently overexpressed in grapevine leaves using the agroinfiltration method. Leaves infiltrated with Agrobacterium tumefaciens cells not carrying any vector served as a mock treatment. Approximately 24 h after transformation, VdMYB1-overexpressing leaves and mock leaves were inoculated with E. necator at 6 day post-inoculation (dpi). Trypan blue staining revealed greater development of PM hyphae on mock leaves than on VdMYB1-overexpressing leaves (Fig. 4a). Next, to quantify fungal reproduction, we determined the number of conidiophores on leaves at 4 and 6 dpi (Fig. 4b). The VdMYB1 overexpressing leaves showed fewer conidiophores than mock leaves both at 4and 6 dpi (Fig. 4b). After PM infection, fungal reproduction was much faster on mock leaves than on VdMYB1-overexpressing leaves (Fig. 4b). Since reactive oxygen species (ROS) play a major role in plant defense against pathogen attack, we analyzed the level of ROS in VdMYB1-overexpressing leaves and mock leaves after inoculation with E. necator. We found that the ROS were strongly induced at 20 min after inoculation in VdMYB1-overexpressing leaves, which reached the peak at 38 mi (Fig. 4c). By contrast, mock leaves did not show crease in the level of ROS after inoculation with E. necator (Fig. 4c). These findings indicate that VdMYB1 enhances resistance to fungal pathogens in grapevine leaves.
Next, we investigated whether transient overexpression of VdMYB1 in grapevine leaves affects transcript levels of defense-related genes. We first monitored transcript levels of VdMYB1 in, three independently transformed leaves showed higher expression level than mock in difference time point (Additional file 2: Figure S1). After challenging the leaves with E. necator, VdMYB transcripts were upregulated (Additional file 2: Figure S1). Salicylic acid-dependent gene PR1 and jasmonate-dependent gene PDF1.2 was also detected. Results showed that there was no difference in VdMYB1 expression level between VdMYB1-overexpressing leaves and mock leaves (Fig. 4d, e).
In grapevine, STS enzymes mediate the biosynthesis of stilbenes and exhibit broad-spectrum resistance to various pathogens . To explore the molecular mechanisms of V. davidii cv. Tangwei resistance to PM, transcriptome sequencing was preformed after challenge with PM. Among of these 10 candidate genes, only one encode stilbene synthase, which showed high homolog with VvSTS2 (GenBank accession no. XM_003634020) (data not published). To investigate whether VdMYB1 regulate VdSTS2, we measured the expression of VdSTS2 in VdMYB1-overexpressing leaves and mock leaves. Transcripts of VdSTS2 increased rapidly following inoculation with E. necator, and peaked at 24 h both in VdMYB1-overexpressing leaves and mock leaves, although VdSTS2 transcripts were more abundant in transgenic VdMYB1-overexpressing leaves than in the mock leaves at all time points (Fig. 4f). Additionally, resveratrol contents in VdMYB1-overexpressing leaves was higher than in mock leaves at 24 and 48 dpi (Fig. 4g). Based on these data, we speculate that VdMYB1 TF activates the expression of VdSTS2.
VdMYB1 targets the STS2 gene promoter
To confirm whether VdMYB1 functions as a TF and targets the promoter of VdSTS2, we conducted in vitro and in vivo assays. Based on the reference genome sequence of V. vinifera cv. Pinot Noir, VdSTS2 promoter region (− 1500–0 bp) was cloned by the homolog clone method. Analysis of the VdSTS2 promoter sequence revealed numerous MYB TF binding sites (MYBBSs) (Fig. 5a). To investigate whether VdMYB1 targets the promoter of VdSTS2 in vitro, we generated a set of serial deletions of the VdSTS2 promoter, and fused these with the GUS reporter gene (Fig. 5a). We also constructed a VdMYB1 overexpression vector as an effector. We then co-transformed the effector and reporter constructs into Arabidopsis leaf protoplasts and measured GUS activity as an indicator of promoter activation. Constructs ProSTSful and ProSTSdel1 containing full-length and deleted variant of the VdSTS2 promoter, respectively, showed higher GUS activity than the ProSTSdel2 construct, which lacked the sequence from --1500 to 500 bp (Fig. 5b). These results indicate that VdMYB1 directly targets the VdSTS2 promoter between − 1500 to − 500 bp.
To further investigate whether VdMYB1 directly binds to the VdSTS2 promoter between − 1500 to − 500 bp in vitro, we performed electrophoretic mobility shift assays (EMSA) with recombinant proteins and nine overlapping oligonucleotide probes encompassing the region from − 1400 to -590 bp of the VdSTS2 promoter (Fig. 5c). The recombinant GST-VdMYB1 protein bound probes containing MYBBSs, but did not bind the probe containing zero MYBBSs, the binding activity of the recombinant protein was correlated with the number of MYBBSs (Fig. 5d). To further verify the transcriptional activation function of VdMYB1, we performed yeast one-hybrid (Y1H) assays with the VdSTS2 gene promoter. Results of Y1H showed that the yeast strain with the bait reporter gene driven by the VdSTS2 promoter grew well on media containing the AbAi antibiotic when co-transformed with the VdMYB1 gene (Fig. 5e). These results show that VdMYB1 function as an R2R3-type MYB transcriptional activator, which binds to the MBSBS in the VdSTS2 promoter and activates the transcription of VdSTS2 gene in grapevine.
VdMYB1 enhances disease resistance by activating the VdSTS2 gene
Next, we performed transient expression assay in tobacco (Nicotiana tabacum) leaves to verify that VdMYB1 activates VdSTS2 expression in a heterologous system. The expression of VdSTS2 under the control of its native promoter enhanced resistance against to Ralstonia solanacearum compared with the mock treatment (grapevine leaves infiltrated with Agrobacterium not carrying any plasmid) (Fig. 6a-c). Tobacco leaves co-expression of VdMYB1 and VdSTS2 were more resistant to R. solanacearum than those expressing only VdSTS2 (Fig. 6a-b). Although there was no difference of the chlorophyll content between the co-expression of VdMYB1 and VdSTS2 leaves and expression VdSTS2 leaves after R. solanacearum infection within 3 days (Fig. 6c). After 6 days, the only expression VdSTS2 leaves showed significant lower chlorophyll content than the co-expression of VdMYB1 and VdSTS2 leaves (Fig. 6c). To explore the basis of this resistance, we examined the transcript level of genes involved in defense responses in transgenic and mock tobacco leaves after inoculation with R. solanacearum. Expression of PR1 and PDF1.2 did not differ between transgenic and mock tobacco leaves (Fig. 7a, b). However, co-expression of VdMYB1 and VdSTS2 produced higher STS2 transcript levels and higher content of resveratrol compared with expression of VdSTS2 alone (Fig. 7c, d). These results indicate that VdMYB1-mediated activation of VdSTS2 increases the content of resveratrol, which enhances defense responses against pathogens.
Transgenic tobacco plants co-expression VdMYB1 and VdSTS2 exhibit resistance to R. solanacearum
To confirm that VdMYB1 enhances disease resistance by activating VdSTS2, we generated stable transgenic tobacco lines co-expressing VdMYB1 and VdSTS2 (Fig. 8a) using Agrobacterium-mediated transformation. Transformed plants were selected by screening for kanamycin resistance and PCR. primers specific to the kanamycin selectable marker gene and VdSTS2 coding sequence yielded a 2, 967 bp fragment in the evaluated T0 transformants (Fig. 8a, c). No similar sized PCR product was obtained from untransformed lines, thus confirming the specificity of PCR primers (Fig. 8c). Transgenic tobacco lines showed higher expression level of VdMYB1 and VdSTS2 than the WT (Additional file 3: Figure S2). Leaves of 7-week-old plants of two transgenic lines and WT plants were inoculated with R. solanacearum. At 48 hpi, VdMYB1 and VdSTS2 transcripts were highly upregulated in transgenic tobacco lines. At 6 dpi, WT plants showed severe wilting symptoms, whereas both transgenic lines displayed less severe disease symptoms (Fig. 8b). To evaluate the progression of disease in R. solanacearum-infected plants, we quantified bacterial population in infected leaves of WT and transgenic tobacco plants at 48 hpi. Significantly lower bacterial growth was observed in transgenic tobacco plants compared with WT plants (Fig. 8d). We also examined chlorophyll content in WT and transgenic leaves inoculated with R. solanacearum. Infection did not change the leaves chlorophyll content of the two transgenic lines (Fig. 8e). However, in WT leaves chlorophyll content showed significant difference (Fig. 8e). Additionally, measurement of resveratrol content showed that transgenic plants were able to synthesize resveratrol, unlike WT plants (Fig. 8f). After inoculation with R. solanacearum, the level of resveratrol significantly higher in transgenic plants than that before inoculation (Fig. 8f).
The MYB TFs play important roles in various the regulatory networks affecting biotic and abiotic stresses, metabolism, and plant development . In grapevine, MYB TFs regulate the flavonoids biosynthesis pathway [12, 13]. However, little is known about how MYB TF mediated transcriptional regulation affects grapevine responses to pathogen. Here, we showed that VdMYB1, an R2R3-type MYB TF from the disease-resistant wild grapevine V. davidii cv. Tangwei, regulates the expression of the VdSTS2 gene by directly binding to its promoter, thus contributing to enhanced resistance against pathogens.
Sequence analyses showed that the VdMYB1 protein is an R2R3-type MYB TF. VdMYB1 localized in the nuclei of Arabidopsis protoplasts and onion epidermal cells and functioned as a VdMYB1 transcriptional activator in yeast. Both these results are typical characteristics of a TF. Most TFs directly target a specific cis-element. The MYB TFs can bind to the MYBBS with the core sequence ACCTACC . In grapevine leaves overexpressing VdMYB1, we found that the expression of key genes in the salicylic acid or jasmonate pathways did not change; however, the transcripts of VdSTS2 gene were more abundant. This surprising finding prompted us to speculate that VdMYB1 targets the promoter of VdSTS2 gene. Several lines of evidence collected in this study support this hypothesis. First, the VdSTS2 promoter contains several variants of the MYBBS, such as MYB2CONSENSUSAT, MYBCORE, MYBGAHV, MYBPLANT, MYBPZM, and MYBST1. Second, overexpression of VdMYB1 in grapevine leaves upregulated VdSTS2 transcripts, and also resulted in increased content of resveratrol. Third, VdMYB1 activated GUS reporter gene expression driven by the VdSTS2 promoter in Arabidopsis protoplasts GUS activity showed that the region with the VdSTS2 promoter with the highest recognition efficiency was between − 1500 and − 500 bp. Analysis of the VdSTS2 promoter using PLACE showed that this core region contains two classic MYBBS. Fourth, EMSAs showed that the VdMYB1 recombinant protein binds to the probe that containing an MYBBS, and the binding activity was correlated with the number of MYBBSs. Lastly, transformation of tobacco leaves showed that STS2 transcripts are more abundant and produce higher contents of resveratrol in leaves co-transformed with VdMYB1 and STS2 under its own promoter.
In grapevine, MYB TFs participate in the regulation of the flavonoid biosynthetic pathway. In cultivated grapevine (V. vinifera), VviMYB5a and VviMYB5b are two homologous R2R3-type MYB TFs that regulate flavonoid biosynthesis [11, 21]. The MYB TFs VviMYBPA1 and VviMYBPA2 also act during seed development for regulating the proanthocyanidin pathway [22, 23]. Another R2R3-type MYB TF,VviMYBF1 regulates the biosynthesis of flavonols . However, there is little information showing that MYB TFs act in defense responses in grapevine. By contrast, MYB TFs in Arabidopsis and other plants have been shown to play important roles in defense responses . In Arabidopsis, the expression of BOS1, an R2R3-type MYB TF, is significantly induced following infection with Botrytis cinerea , and the bos1 mutant exhibits increased susceptibility to B. cinerea, Alternaria brassicicola, Pseudomonas syringae pv tomato, and Peronospora parasitica . Another R2R3-type MYB-related protein and a putative TF in Arabidopsis thaliana, MYB30, positively regulates programmed cell death associated with hypersensitive response . In this study, we showed that transcript levels of VdMYB1 were significantly increased after inoculation with the PM fungus, E. necator. Transient overexpression of VdMYB1 in grapevine leaves resulted in resistance to PM and the production of abundant ROS within 38 min of infection. More recent studies showed that SA promoted the biosynthesis of flavan-3-ol and PAs in Poplar by activating the MYB-bHLHWD40 complex against rust proliferation and infection . The present finding also support author study which concluded that SA increased flavonoid accumulation in plant by inducing MYB TFs.
The STS enzyme participates in the biosynthesis of stilbenes, which function as antimicrobial compounds. Expression of VdMYB1 in grapevine leaves led to the accumulation of more STS transcripts and production of high levels of resveratrol. Moreover, infection of VdMYB1-overexpressing grapevine leaves with PM resulted in significantly higher STS compared with mock. Furthermore, transient expression of the VdSTS2 under the control of its native promoter showed that the transformed tobacco leaves were more resistant to R. solanacearum compared with the mock. The resistance to R. solanacearum was even higher in leaves co transformed with VdMYB1 and VdSTS2. Reactive oxygen species (ROS) play a central role in plant defense against various pathogens . By analyzing the ROS accumulation in VdMYB1-expressing plants, we found that the contents of H2O2 were induced upon PM infection, while the control plants were maintained at lower levels. These findings indicate that VdMYB1 may play a role in the regulation of ROS production. ROS was accumulated at the pathogen attack site in plants, which called oxidative burst, is can lead to a hypersensitive response (HR) that prevents biotrophic pathogens further spread . Recent research has indicated that ROS not only function as toxic compounds that lead HR, but also act important early signal molecules . Duan et al., proposed that ROS are necessary for the induction of stilbene synthase, and resulted in production of proanthocyanidin . Jiao et al., reported that the accumulation of trans-resveratrol, trans-piceid and cis-piceid markedly increased after treatment with H2O2 . We proposed that overexpression VdMYB1 resulted in plant accumulation ROS to prevent pathogen invasion.
Most MYB TFs directly target the promoters of downstream genes. TRANSPARENT TESTA 2 (TT2), an Arabidopsis R2R3 MYB domain protein, provides target specificity to the MYB-bHLH-WD complex, which activates various phytoalexin biosynthetic genes, including DFR, TT12, AHA10, and BAN . The MYB TF MYB134 in poplar shows strong sequence similarity to Arabidopsis TT2, and overexpression of MYB134 activates the phytoalexin biosynthesis pathway and significantly increase phytoalexin levels in poplar . Results of EMSA show that recombinant MYB134 protein binds to the promoter regions of genes in the phytoalexin pathway . In grapevine, VviMYB5a and VviMYB5b activate the expression of VviCHI, VviF3’5’H, and VviANS, which encode enzymes of the general flavonoid biosynthesis pathway and participate in synthesis of phytoalexins, anthocyanins, and flavonols, respectively [12, 21]. In addition, VviMYB5b activates the expression of VviLAR1 and VviANR, whereas VviMYB5a is implicated only in the regulation of VviLAR1 expression [12, 13]. Two MYB proteins in grapevine, VviMybPA1 and VviMybPA2, appear to be the closest orthologues of Arabidopsis TT2 [22, 23]. VviMybPA1 or VviMybPA2 significantly upregulate the expression of genes encoding enzymes of the flavonoid biosynthesis pathway, including ANR and LAR1 [22, 23]. Additionally, VviMYBF1 activates the expression of the flavonol-specific gene VviFLS1 but not that of VviUFGT or VviANR . The MYB TF VviMYB14 and its homolog VviMYB15 co-express with VviSTS in leaf tissues under biotic and abiotic stresses and in berries during maturation . Transient expression of VviMYB14 and VviMYB15 results in enhanced transcript levels of VviSTS . In the grapevine hairy root system, overexpression of VviMYB15 results in higher VviSTS transcript levels and the accumulation of glycosylated stilbenes .
Together, these data confirm that VdMYB1 TFs regulate the stilbenoids biosynthesis pathway at the transcriptionally level. Our results showed that VdMYB1 binds to the VdSTS2 promoter both in vivo and in vitro, and overexpression of VdMYB1 in grapevine and tobacco leaves resulted in increased accumulation of VdSTS2 transcripts. High levels of VdSTS2 transcripts, and consequently increase in resveratrol content, resulted in greater resistance to E. necator in grapevine leaves and to R. solanacearum in tobacco leaves.
V. davidii cv. Tangwei were cultivated in the experimental vineyard of the germplasm repository for grapes in the Institute of Botany, Chinese Academy of Sciences in Beijing.
RNA extraction and VdMYB1 cDNA isolation
Total RNA was extracted from the leaves of V. davidii cv. Tangwei, as described previously by Yu et al. . Total RNA was treated with RNase-free DNase I for 30 min to remove traces of contaminating genomic DNA, and used for cDNA synthesis using SMARTer RACE cDNA Amplification Kit (Clontech, Japan), according to the manufacturer’s instructions. The VdMYB cDNA was isolated by PCR using degenerate primers designed based on the partial MYB sequence of V. vinifera and Ex Taq HS DNA polymerase (Takara, Dalian, China). Subsequently, the sequence of the amplified partial cDNA fragment was used for designing gene specific primers (Additional file 1: Table S1), which were used for performing RACE PCR according to the manufacturer’s instructions. PCR products obtained from all successful amplifications were cloned into the using pGEM-T Easy vector (Promega, Beijing, China) and five independent clones has sequenced.
Total RNA was extracted from treated grapevine leaf and all tissues as described above. To generate first-strand cDNA, 0.5 μg of DNase I-treated total RNA was reverse transcribed in 10 μL volume using PrimeScript Reverse Transcriptase Kit according to the manufacturer’s instructions (TaKaRa, Dalian, China). Subsequently, qRT-PCR was performed on a Bio-Rad IQ5 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) using SYBR Premix Ex Taq II, according to the manufacturer’s instructions (TaKaRa, Dalian, China). Primers used for qRT-PCR are listed in Additional file 1: Table S1. PCR reactions were prepared in 96-well plates (Bio-Rad, USA), and each reaction contained 1 μL of diluted cDNA (100 ng/μL), 12.5 μL of SYBR Green PCR Master Mix, 1 μL of each primer (at 250 nM) in a final volume of 25 μL. The qRT-PCR was performed using the following conditions: initial denaturation at 94 °C for 3 min, followed by 40 cycles of denaturation at 94 °C for 15 s, annealing at 58 °C for 30 s, and extension at 72 °C for 30 s, and lastly melting curve analysis at 60–94 °C. The VdGAPDH gene was used as a reference gene for data normalization according to the 2-ΔΔc(t) method . All reactions were conducted in three technical replicates. Mean values of three independent experiments were analyzed using the Student’s t-test.
Subcellular localization and transcriptional activation assays
The VdMYB1 ORF minus the stop codon was cloned into the pBI221-GFP vector using XbaI and KpnI restriction sites. In the resulting vector pBI221-GFP/VdMYB1, the GFP-VdMYB1 fusion was driven by the CaMV 35S promoter. Primers used for cloning are listed in Additional file 1: Table S1. Sequence analysis was used to verify the final construct. Gene gun bombardment and polyethylene glycol (PEG) transformation methods were used to introduce the constructs (including the vector-only negative control) into onion epidermal cells and Arabidopsis protoplasts as described previously . Transformed cells were incubated in a growth chamber at 24 °C for 16–18 h. GFP signals were detected by confocal laser scanning microscopy (Zeiss LSM 510, Oberkochen, Germany). At least three replicates were performed for all transformations.
To generate the pGBKT7/VdMYB1 fusion construct, the VdMYB1 ORF minus the stop codon was cloned into the pGBKT7 vector using NdeI and BamH I restriction site. Full-length GAL4 gene sequence from the pCL1 plasmid was cloned into the pGBKT7 vector to serve as the positive control (pGBKT7/GAL4), and the pGBKT7 vector was used as the negative control. Primers used for PCR are listed in Additional file 1: Table S1. The final constructs were verified by sequencing. The control plasmid and the pGBKT7/VdMYB1 were transformed into yeast (strain AH109). After transformation, yeast cells were cultured on SD/−Trp medium at 28 °C for 3 days. Three transformants were then cultured on SD/−His/−Ade/−Trp medium with X-α-gal at 28 °C for 3 days.
Agrobacterium-mediated transient expression assay
The VdMYB1 ORF was cloned into pBI121 binary vector to generate the construct pBI121/VdMYB1 for transient expression assay. The VdSTS2 promoter sequence (− 1500 to 0) was amplified from genomic DNA. A series of nested 5′ deletions of the VdSTS2 promoter was generated by PCR using sequence-specific primers (Additional file 1: Table S1). Three STS promoter sequences (ProSTSful, ProSTSdel1, and ProSTSdel2) were cloned into the pC0390GUS vector using PstI and EcoRI restriction site to generate ProSTSful::GUS, ProSTSdel1::GUS, and ProSTSdel2::GUS, respectively. The recombinant plasmid was confirmed by sequencing. All constructs were transformed into Agrobacterium tumefaciens strain GV3101 by electroporation. Leaves of 8-week-old V. vinifera cv. Carignane plants or 6-week-old tobacco plants in vitro were detached and used for transformation, according to the methods described by Xu  and Sparkes . GUS activity was measured as described previously [38, 39], and each GUS assay was performed at least three times. The amount of ROS was measured using a luminol-based assay, as described by Keppler et al. . Resveratrol in tobacco leaves was measured by HPLC analysis. Total chlorophyll was extracted using 80% acetone and measured at 645 nm and 663 nm absorbance.
Agrobacterium-mediated transformation of tobacco plants
The ProVdSTS2:VdSTS2 cassette containing SacI and BamHI restriction sites was ligated into pCAMBIA2300 binary vector, to generate the construct pC2300/VdSTS2. The VdMYB1 ORF was cloned into pC2300/VdSTS2, to generate the stable transformation vector pC2300-VdSTS2-VdMYB1. For tobacco transformation, the binary vector pC2300-VdSTS2-VdMYB1 was introduced into Agrobacterium strain LBA4404. Leaf discs (8 mm diameter) collected from fully expanded leaves of tobacco plants were used for Agrobacterium-mediated transformation. Putative transformants were screened by PCR using genomic DNA isolated from WT plants and various transgenic lines as template.
All five MYBBSs in the VdSTS promoter sequence (− 1400 to − 590) were mutated using the MutanBEST Kit (Takara, Dalian, China) to generate MYBBSm1–5 mutant fragments. To perform EMSAs, MYBBS and MYBBSm1–5 probe fragments were obtained by PCR amplification and labeled with [γ-32P] ATP using T4 polynucleotide kinase (New England Biolabs, Hitchin, UK). The VdMYB1 ORF was cloned into the pGEX-6P-1 vector, and transformed into Escherichia. coli strain BL21(DE3). IPTG was added to the bacterial culture to induce expression of the GST-VdMYB1 fusion protein. The expressed fusion proteins were purified using GST SpinTrap columns (GE Healthcare). Labeled probes were incubated with 50 ng of GST-VdMYB1 in binding buffer (10 mM Tris [pH 7.5], 5 mM MgCl2, 50 mM KCl, 100 μg/mL BSA, 2.5% glycerol, 1 mM DTT, and 50 ng/μL poly (dI-dC) for 25 min. The fusion protein-bound probes were separated from unbound probes using PAGE (5%). Gels were dried, and signal was detected by overnight exposure to X-ray film (Kodak).
Yeast one-hybrid assay
The yeast one-hybrid assay was performed using MATCHMAKER Gold Yeast One-Hybrid Library Screening System (Clontech) and YEASTMAKER Yeast Transformation System 2 (Clontech). The amplified promoter regions were cloned upstream of the Aureobasidin A resistance (AUR1-C) reporter gene in the pAbAi vector to generate the following constructs: pAbAi-proVdSTS2ful, pAbAi-proVdSTS2del1, pAbAi-proVdSTS2del2. The VdMYB1 ORF was cloned in frame after the GAL4 transcriptional activation domain (AD) in pGADT7, and the resulting AD-VdMYB1 was co-introduced with other pAbAi vectors into the yeast strain Y1HGold. The transformed yeast cells were cultured on SD medium containing 0 or 200 ng/ml Aureobasidin A at 30 °C for 3 days. The β-galactosidase activity of positive clones was identified according to the manufacturer’s instructions.
Determination of Trans-resveratrol content by HPLC method
Trans-resveratrol levels in berry skins and leaves were measured using HPLC method. The standard for trans-resveratrol was purchased from Sigma-Aldrich (USA). Mocked leaves and VdMYB overexpressed leaves inoculated with E. necator for 0 and 24 h. One gram samples were ground to powder using a porcelain mortar and pestle in liquid nitrogen, extracted by 15 mL extraction solution (methanol: ethyl acetate, 1:1 v/v) for 24 h at room temperature in the dark. After centrifugation at 20000 g at 4 °C for 10 min, the supernatant was evaporated at 40 °C until the solvent was volatilized completely and then dissolved in 2 ml methanol. The extract was filtered through a 0.22 μm PTFE membrane filter before resveratrol analysis. Extractable amounts of resveratrol were analyzed using a Waters e2695 HPLC system (USA). Elution was carried out with a mobile phase delivered using a Waters C18 HPLC pump at a flow rate 0.8 mL·min− 1. A Waters 2996 UV detector was used at 306 nm. Mean values and standard deviations were obtained from three biological replicates.
Determination of chlorophyll content
Leaf tissues were ground in liquid nitrogen and 100 mg of leaf powder was used to extract pigments using 80% acetone. Chlorophyll absorbance was analyzed by spectrophotometry at 645 and 663 nm.
Pathogen inoculation and SA, MeJA treatments
The PM pathogen E. necator was maintained on V. vinifera cv. Carignane seedlings. The inoculation of V. davidii cv. Tangwei leaves with E. necator was performed as described previously . To monitor the growth of E. necator, conidiophores were counted as described previously . Ralstonia solanacearum strain BJ1057 was cultured in tetrazolium agar medium at 28 °C. A needleless hypodermic syringe was used to infiltrate bacterial suspensions (104 cfu/ml) into leaves of intact tobacco plants. The growth of R, solanacearum was measured at 5 dpi . Mean values of three independent experiments were used for statistical analysis using Student’s t-test.
For exogenous chemical compounds treatments, 100 μM SA, and 0.5 g/L MeJA were sprayed on the selected grapevine leaves according to the method described , and was repeated three times on three independent plants. Leaves sprayed with sterile water were used as negative controls. After 0, 12, 24, 36, and 48 h of inoculation, the treated grapevine leaves were sampled, immediately frozen in liquid nitrogen, and stored at − 80 °C for further study.
Nucleotide sequence and putative amino acid sequence were analyzed with the basic local alignment search tool (BLAST) at the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov) and Grape Genome website (http://www.genoscope.cns.fr/externe/GenomeBrowser/Vitis/, V2 version). Phylogenetic tree was constructed using neighbor joining method with the bootstrap values of 1000 by MEGAX. Promoter analysis was performed using online analysis software of PLACE and PlantCARE. Protein domain analysis was done by the online software of SMART (http://smart.embl-heidelberg.de/) and ExPASy (https://www.expasy.org/).
Availability of data and materials
All data generated and analyzed during this study are included in this published article.
Auto-inhibited H+-ATPase isoform 10
- ANR :
- CaMV 35S:
Cauliflower mosaic virus 35 s promoter
- CHI :
- DFR :
Electrophoretic mobility shift assay
- F3’5’H :
Flavonol synthase 1
Green fluorescent protein
- GUS :
Leucoanthocyanidin reductase 1
MYB binding site
NAM, ATAF, and CUC
Open reading frame
- PDF1.2 :
Plant defensin 1.2
Rapid amplification of cDNA ends
Reactive oxygen species
SWI3, ADA2, N-CoR, and TFIIIB domains
Simple modular architecture research tool
TRANSPARENT TESTA 2
- UFGT :
Vitis davidii MYB1
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We thank Dr. Jennifer Mach for her language editing and critical reading of the manuscript.
This work was supported by National Natural Science Foundation of China (U1504321, 31701893, 31672106, C150102), China Postdoctoral Science Foundation (2015 M570174), Program for Science &Technology Innovation Talents in University of Henan Province (13HASTIT004) and Innovative Research Team in Henan University of Science & Technology (2015TTD003).
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Table S1. The sequences of the primers used in these experiments. (DOCX 15 kb)
Figure S1. Relative expression level of VdMYB1 in different VdMYB1-overexpressing transgenic lines. (DOCX 555 kb)
Figure S2. VdSTS2 and VdMYB1 expression level in stable transgenic tobacco plants co-overexpressing VdSTS and VdMYB1. (DOCX 377 kb)
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Yu, Y., Guo, D., Li, G. et al. The grapevine R2R3-type MYB transcription factor VdMYB1 positively regulates defense responses by activating the stilbene synthase gene 2 (VdSTS2). BMC Plant Biol 19, 478 (2019). https://doi.org/10.1186/s12870-019-1993-6
- MYB transcription factor
- Defense responses
- Stilbene synthase
- Powdery mildew