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Fig. 8 | BMC Plant Biology

Fig. 8

From: The grapevine R2R3-type MYB transcription factor VdMYB1 positively regulates defense responses by activating the stilbene synthase gene 2 (VdSTS2)

Fig. 8

Stable transgenic tobacco plants overexpressing VdSTS2 and VdMYB1 show increased resistance to R. solanacearum. a Schematic representation of constructs used for tobacco transformation. B, right border; LB, left border; CaMV 35S, CaMV 35S promoter; 35S polyA, CaMV 35S polyA terminator. b Transgenic tobacco plants show enhanced resistance to R. solanacearum. The phenotype of WT and transgenic tobacco plants is shown in the top panel, and disease symptoms on the leaves of WT and transgenic lines at 6 dpi are shown in the bottom panel. c Identification of transgenic lines by PCR amplification of genomic DNA. d Growth of bacterial population in R. solanacearum-inoculated transgenic and WT plants at 48hpi. e Chlorophyll content in WT and transgenic plants at 0dpi and 6 dpi. f Measurement of trans-resveratrol content in WT and transgenic tobacco plants after R. solanacearum infection using HPLC. Error bars represent SD. Asterisks indicate statistically significant differences (**P < 0.01; Student’s t-test)

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