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Fig. 3 | BMC Plant Biology

Fig. 3

From: The grapevine R2R3-type MYB transcription factor VdMYB1 positively regulates defense responses by activating the stilbene synthase gene 2 (VdSTS2)

Fig. 3

Subcellular localization and transcriptional activation function of VdMYB1. VdMYB1 expression in onion epidermal cells (a) and Arabidopsis protoplasts (b). The expression of VdMYB1-GFP fusion protein was detected usingconfocal laser -scanning microscopy 16 h after transformation. c Transcriptional activation function of VdMYB1. The constructs pGBKT7/VdMYB1, pCL1 (positive control) and pGBKT7 (empty vector; negative control) were expressed in the yeast strain AH109. Transformants were incubated on SD/−Trp, SD/−Trp/−His/−Ade, and SD/−Trp/−His/−Ade/+X-α-gal media, and the growth of yeast cell and tβ-galactosidase activity were measured

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BMC Plant Biology

ISSN: 1471-2229

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