Skip to main content
Download PDF

Ostkpr1 functions in anther cuticle development and pollen wall formation in rice

BMC Plant Biology volume 19, Article number: 104 (2019) Cite this article

Abstract

Background

During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function of each of these components in different species remains unclear.

Results

In this study, we characterized the function of OsTKPR1, a rice orthologue of Arabidopsis TKPR1. Loss of function of OsTKPR1 delayed tapetum degradation, reduced the levels of anther cuticular lipids, and impaired Ubisch body and pollen exine formation, resulting in complete male sterility. In addition, the phenylpropanoid pathway in mutant anthers was remarkably altered. Localization studies suggest that OsTKPR1 accumulates in the endoplasmic reticulum, while specific accumulation of OsTKPR1 mRNA in the anther tapetum and microspores is consistent with its function in anther and pollen wall development.

Conclusions

Our results show that OsTKPR1 is indispensable for anther cuticle development and pollen wall formation in rice, providing new insights into the biochemical mechanisms of the conserved sporopollenin metabolon in flowering plants.

Background

Pollen grains of land plants are surrounded by a complex multilayered cell wall, the so called pollen wall, which offers protection from biotic and abiotic environmental stresses and plays an important role during the interaction between male and female organs [1, 2]. The typical pollen wall in flowering plants is composed of two distinct layers: the outer exine and inner intine, with the pollen coat deposited on the surface of the exine [2, 3]. The exine can be further divided into the sexine (outer) and nexine (inner), and the sexine itself also contains two layers: an outer tectum layer and an inner layer of vertical regular arrested bacula between the tectum and the nexine [4]. Mutants with defective pollen wall structures often show reduced levels or complete loss of fertility [5, 6].

The highly sculptured exine is mainly composed of sporopollenin, whose chemical composition is still an enigma [1]. In general, sporopollenin is a complex biopolymer consisting of fatty acids and phenolic compounds, covalently connected by ester or ether linkages [5, 7]. Precursors of sporopollenin are known to be produced and secreted from the tapetal cells to the surface of pollen grains [7]; however, some evidence also demonstrates that pollen grains themselves contribute to sporopollenin biosynthesis and exine formation [8]. In tapetal cells, the de novo biosynthesis of sporopollenin precursors occurs in plastids, and the deposition of sporopollenin precursors begins soon after the completion of meiosis when the temporary callose wall is degraded and the primexine is formed (stage 9). The deposition of sporopollenin precursors continues until microspore vacuolation, and is largely completed by the time of pollen mitosis (stage 12) [9]. Sporopollenin biosynthesis and deposition coincides with tapetum programmed cell death (PCD), which occurs from stage 8a until stage 10 [4], suggesting a possible relationship between these two events. Immature or delayed tapetum PCD usually disrupts pollen wall formation and causes defects in male fertility [10,11,12].

Recent genetic and biochemical studies in plants, mainly from two model plants Arabidopsis and Oryza sativa (rice), have significantly increased our understanding of pollen wall formation [6, 13]. Because the biopolymer sporopollenin is found to be conserved in flowering plants, moss, ferns and even fungi [14, 15], the biosynthesis and deposition of sporopollenin monomers therefore represents one of the most ancient biochemical pathways in flowering plants [16,17,18]. Consistent with this, related orthologous genes involved in pollen wall formation are found in dicots, monocots, gymnosperms, and even the moss, such as MS2 (Male Sterile 2)/DPW (Defective Pollen Wall) [19, 20], CYP703A2/CYP703A3 [18, 21], CYP704B1/CYP704B2 [22, 23], ABCG26/OsABCG15 (PDA1) [24, 25], ACOS5 (Acyl-CoA Synthetase 5)/OsACOS12/NtACOS1 [12, 26, 27], LAP6 (Less Adhesive Pollen 6)/PKSA (Polyketide Synthase A)/OsPKS1/NtPKS1, LAP5/PKSB/OsPKS2 [16, 17, 27,28,29,30], TKPR1 (Tetraketide α Pyrone Reductase 1)/NtKPTR1/OsTKPR1 [27, 31], and AMS (Aborted Microspores)/TDR (Tapetum Degeneration Retardation) [10, 11]. The fact that most of these reported orthologous genes are mainly expressed in tapetum and/or microspores suggests that there is a conserved male organ tissue-specific regulatory network controlling pollen wall formation in flowering plants.

In Arabidopsis, the plastid de novo synthesized fatty acids are first activated by ACOS5 to generate fatty acyl CoAs [26], and the resulting fatty acyl CoAs are then condensed with malonyl-CoA by PKSA/LAP6 and/or PKSB/LAP5 to produce α-pyrone products [16, 17], which are further reduced by TKPR1 and TKPR2 to yield hydroxylated tetraketide compounds [31]. All intermediate and final metabolites in this pathway, including fatty acyl CoAs, α-pyrones and hydroxylated tetraketides, serve as important resources for sporopollenin precursors. Genetic and biochemical studies reveal that ACOS5, PKSA/B (LAP6/LAP5), and TKPR1 form a sporopollenin metabolon in the Arabidopsis tapetum for pollen wall formation [32]. Although this metabolon is also found to be present in tobacco [27], rice [27], moss [33], Hypericum perforatum [34] and canola [35], detailed genetic and biochemical evidence is needed to elucidate the specific or common roles of the components in pollen wall formation in various plant species.

The rice genome contains single copies of genes encoding ACOS5 (OsACOS12), PKSA/LAP6 (OsPKS1), PKSB/LAP5 (OsPKS2), and TKPR1 (OsTKPR1). Mutation of any one of these genes significantly affects male fertility in rice; for example, osacos12 [12, 36, 37], ospks2 [28, 30], and ostkpr1 [27] are completely male sterile, while ospks1 is partially fertile [27]. This suggests that the ACOS5-PKSA/B-TKPR1 sporopollenin metabolon is likely to be conserved between rice and Arabidopsis. Nevertheless, some reports also indicate that each component of this metabolon may have divergent functions in different species. For example, unlike ACOS5 in Arabidopsis, OsACOS12 affects tapetum PCD and anther cuticle formation in rice [12, 36]. Moreover, PKSB/LAP5 and OsPKS2 have different effects on anther phenylpropanoid metabolism [16, 28]; pksb/lap5 in Arabidopsis are partially male sterile, while ospks2 in rice is completely male sterile [28]. A previous study reported that the osptkpr1 mutant (carrying a T-DNA insertion in the second exon of LOC_Os09g32020) lacks mature pollen grains and does not produce seeds [27], however, the mechanism by which OsTKPR1 controls fertility remains unresolved.

In this study, we characterized a novel mutant of OsTKPR1 in rice, namely ostkpr1–2, which was completely sterile, displaying delayed tapetum degradation, impaired Ubisch body patterning, reduced anther cuticular profiles, and aborted pollen grains with defective exine. The phenylpropanoid profile of ostkpr1–2 anthers was remarkably altered, which was distinct from that in oslap5/ospks2 anthers. Our studies suggest that OsTKPR1 functions in anther cuticle development and pollen wall formation, and reveal some interesting putative differences compared to TKPR1 from Arabidopsis.

Results

Isolation and characterization of the ostkpr1–2 mutant

Screening of a 60Co γ-ray radiated mutant library in the background of 9522, a cultivar of O. sativa ssp japonica, led to the isolation of a male sterile mutant, ostkpr1–2, which was named based on the results presented below. The mutant exhibited normal vegetative development (Fig. 1a) with normal inflorescences and spikelets (Fig. 1b, c), but was completely male sterile with smaller and paler anthers containing neither mature nor viable pollen grains as evidenced by iodine potassium iodide (I2-KI) staining (Fig. 1d-g). All F1 plants of crosses between WT and the mutant using WT pollen were fertile, and resulting F2 plants exhibited a segregation ratio of approximately 3:1 for fertility: sterility (fertile: sterile = 93:29, χ2 = 0.132, P > 0.05, χ2 test used), indicating that ostkpr1–2 is a recessive, fully penetrant male sterile mutant.

Fig. 1
figure 1

Phenotypic comparisons between WT and the ostkpr1–2 mutant. a Plants after bolting. b Rice panicles at the heading stage. c Spikelets at the heading stage. d Spikelets after removal of the lemma and palea. e Anther at stage 13 of WT. f Stage 13 WT pollen grains stained with 2% I2-KI solution. Mature pollen grains are stained. g Anther at stage 13 of ostkpr1–2 mutant. h Contents of stage 13 anthers of ostkpr1–2 stained with 2% I2-KI solution showing no pollen grains. gl, glume; le, lemma; pa, palea; st, stamen. Bars = 2 mm in c, 1 mm in d and e, and 100 μm in f and g

Full size image

Phenotypic analysis of ostkpr1–2

Semi-thin cross sections from anthers of various developmental stages were compared to identify possible reproductive developmental defects in ostkpr1–2. No obvious defects in anther development could be observed in ostkpr1–2 compared to WT at tetrad stage (stage 8b), where typical four anther wall layers and tetrads are formed in both anther locules (Fig. 2a, f). At stage 9, WT microspores were regular in shape (oval), evenly distributed in the anther locule, and tapetal cells became condensed (Fig. 2b), while ostkpr1–2 microspores seemed to be less compact with irregular shape and closely aggregated in the anther locule, and the tapetum cells had not expanded (Fig. 2g). At stage 10, WT microspores expanded and became highly vacuolated, and the tapetum appeared much thinner due to degradation (Fig. 2c), while ostkpr1–2 microspores were shrunken without vacuolation and the tapetum appeared to have persisted without degradation (Fig. 2h). From stage 11 to stage 12, WT mature pollen grains formed in the anther locule after two rounds of mitotic division, and the tapetum had degraded completely into cellular debris (Fig. 2d, e). In contrast, the microspores of ostkpr1–2 had degraded, leaving only debris inside the shriveled anther locules (Fig. 2i, j).

Fig. 2
figure 2

Transverse thin-section comparison of anther development between WT and ostkpr1–2 showing locules from the anther section of WT (a-e) and ostkpr1–2 (f-j). a and f stage 8b. b and g stage 9. c and h Stage 10. d and i early stage 11. e and j stage 12. E, epidermis; En, endothecium; M, middle layer; T, tapetal layer; Msp, microspores; DMsp, degraded microspore; Mp, mature pollen; Deb, debris. Bars = 15 μm

Full size image

At stage 13, scanning electron microscopy (SEM) revealed additional defects in anther and pollen development. The outer surface of WT anthers was typically covered with a thick layer of sculptured nanoridges (Fig. 3a), while the inner surface of WT anthers was covered with plenty of evenly distributed Ubisch bodies (Fig. 3c). In contrast, the outer surface of ostkpr1–2 anthers was covered with a much thinner layer of smooth nanoridges (Fig. 3b), while the inner surface of ostkpr1–2 anthers was covered with fewer and irregularly distributed Ubisch bodies (Fig. 3d). While an elaborate exine pattern appeared on the surface of WT pollen grains (Fig. 3e, g), aggregated irregularly-distributed particles were present on the shrunken ostkpr1–2 pollen surface (Fig. 3f, h). SEM observation indicated that OsTKPR1 is indispensable for anther cuticle and pollen exine formation in rice.

Fig. 3
figure 3

Scanning electron microscopic examination of anthers and pollen in WT and the ostkpr1–2 mutant. a and b, Anther outer surface of WT (a) and ostkpr1–2 (b) at stage 13. c and d, Anther inner surface of WT (c) and ostkpr1–2 (d) at stage 13. e and f, The morphology of a mature WT (e) and ostkpr1–2 (f) pollen. g and h, Enlarged image of the mature pollen surface of WT (g) and ostkpr1–2 (h). Bars = 20 μm in e and f; 10 μm in a to d; 5 μm in g and h

Full size image

To further examine the early morphological changes occurred in ostkpr1–2 anthers and microspores, transmission electron microscopy (TEM) was used. At stage 9, a dark-stained cuticle was present at the surface of the WT anther (Fig. 4a), and typical Ubisch bodies had formed on the WT anther inner surface (Fig. 4b). At this stage, WT microspores with regular pollen walls had been released from tetrads (Fig. 4c), and the sexine together with bacula appeared on the microspore plasma membrane (Fig. 4d). At the corresponding stage in ostkpr1–2 mutants, the cuticle showed weak staining (Fig. 4e), while fewer and smaller Ubisch bodies (Fig. 4f) were present on the outer and inner surfaces of ostkpr1–2 microspores, respectively. The released ostkpr1–2 microspores had a weakly-stained pollen wall (Fig. 4g) with fewer bacula and sexine structures (Fig. 4h). At stage 10, the WT tapetum had degenerated, microspores were highly vacuolated (Fig. 4i), and Ubisch bodies exhibited a characteristic shape with high electron density (Fig. 4j). By contrast, in ostkpr1–2 anthers the tapetum remained present, the microspores did not become vacuolated (Fig. 4m), and Ubisch bodies seemed to be normally shaped but with less electron density (Fig. 4n). At this stage, the exine of the WT pollen was thick (Fig. 4k) with two typical, well-organized electron-dense layers (Fig. 4l), while the exine of ostkpr1–2 pollen was very thin (Fig. 4o) with two thin electron-dense layers (Fig. 4p). The TEM results support the results obtained from semi-thin section and SEM analyses, highlighting the important roles of OsTKPR1 in formation of the rice anther cuticle, Ubisch bodies, and pollen exine, and for the timely tapetum degradation.

Fig. 4
figure 4

Transmission electron microscopy (TEM) analysis of anthers, microspores and pollen exine in WT and ostkpr1–2. a and e Anther at stage 9 of WT and ostkpr1–2. Bars: 5 μm. b and f Tapetal cells at stage 9 of WT and ostkpr1–2. Bars: 1 μm. c and g Microspores at stage 9 of WT and ostkpr1–2. Bars: 2 μm. d and h, Pollen exine at stage 9 of WT and ostkpr1–2. Bars: 0.5 μm. i and m Anther at stage 10 of WT and ostkpr1–2. Bars: 5 μm. j and n Tapetal cells at stage 10 of WT and ostkpr1–2. Bars: 0.5 μm. k and o Microspores at stage 10 of WT and ostkpr1–2. Bars: 2 μm. l and p Pollen exine at stage 9 of WT and ostkpr1–2. Bars: 0.5 μm. Ba, Bacula; C, cuticle; E, epidermis; En, endothecium; Ex, exine; Ml, middle layer; Msp, microspores; Ne, Nexine; T, tapetal layer; Te, Tectum; Ub, Ubisch body

Full size image

Map-based cloning of OsTKPR1

The OsTKPR1 gene was first mapped between two indel molecular markers chr9–3485 and chr9–6308 on chromosome 9 and eventually mapped between 69.5 and 92.1 centimorgans (CMs) with molecular markers chr9–4979 and chr9–6308 (Fig. 5a). Using a high throughput re-sequencing technique and PCR, a 15 nucleotide deletion was identified in the fourth exon of LOC_Os09g32020, which is predicted to result in the removal of 5 amino acids (serine, leucine, serine, histidine and glutamic acid) and the change of one amino acid from proline into glutamine (Fig. 5b). LOC_Os09g32020 was previously annotated as OsDFR2 (Dihydroflavonol-4-reductase-like protein) and identified as an anther-specific gene down-regulated in a thermo-sensitive male sterile mutant in rice [38]. An allelism test with a homozygous ostkpr1 T-DNA insertion mutant PFG_2B-00257 confirmed that the mutation in LOC_Os09g32020 almost certainly causes the developmental defects in ostkpr1–2 (Additional file 1: Table S1). The ostkpr1 mutant displayed similar reproductive defective phenotypes to that of ostkpr1–2, namely, complete male sterility with a defective anther cuticle and abnormal pollen exine (Additional file 2: Figure S1).

Fig. 5
figure 5

Molecular identification of OsTKPR1. a Fine mapping of the OsTKPR1 gene on chromosome 9. Names and positions of molecular markers and the recombinants are indicated. The OsTKPR1 locus was mapped to a 22.6 centimorgan region between two molecular markers (chr9–4979 and chr9–6308). b Schematic representation of the exon and intron organization of OsTKPR1. The mutant sequence has a 15-nt deletion in the fourth exon (indicated by red colored characters in the wild-type sequence and a red arrow in the schematic figure below), causing the deletion of five amino acids (Ser, Leu, Ser, His, Glu) and alteration of one amino acid (from Pro to Gln). Blue boxes indicate exons, intervening lines indicate introns

Full size image

Expression pattern of OsTKPR1

In silico analysis using a publically available microarray database (http://ricexpro.dna.affrc.go.jp/data-set.html) revealed that OsTKPR1 is specifically expressed in anthers at early developmental stages (Additional file 3: Figure S2). This digital expression pattern of OsTKPR1 was confirmed by quantitative RT-PCR with anthers at different developmental stages and other tissues of WT plants, which revealed that OsTKPR1 expression could only be detected in anthers at stage 9 and 10 (Fig. 6a). The precise spatial and temporal expression pattern of OsTKPR1 was further confirmed by in situ hybridization using WT anther sections, in which OsTKPR1 signal was observed mainly in the microspores and, to a less extent, in the tapetum (Fig. 6b, c). These expression results confirm that OsTKPR1 is closely associated with the development of anther and pollen in rice at early stages.

Fig. 6
figure 6

Expression analysis of OsTKPR1. a qPCR analysis of the OsTKPR1 transcript. St 7 to St 13 correspond to anthers at stages 7 to stage 13, respectively. The result is shown as the mean ± standard derivation of three biological replicates. b-e In situ localization of OsTKPR1 expression with digoxigenin-labelled antisense (b and c) and sense (d and e) RNA probe in the WT anther sections at stage 9 (b and d) and stage 10 (c and e). Bars = 20 μm

Full size image

Subcellular location of OsTKPR1 protein

Arabidopsis TKPR1, the orthologous protein of OsTKPR1, was previously reported to be an important part of the sporopollenin metabolon, acting in the ER of tapetal cells to influence sporopollenin biosynthesis and pollen formation [31]. Given the similarity of these two proteins (63% identity revealed using Clustal X; Additional file 4: Figure S3) and the comparable deficiencies in pollen exine formation in both ostkpr1–2 and tkpr1 mutants, we considered whether OsTKPR1 protein might also localize to the ER. To test this hypothesis, confocal microscopy was used to observe Nicotiana benthamiana (tobacco) leaves that were transiently transformed with an OsTKPR1-GFP construct under the control of the CaMV35S promoter. The results confirmed that the GFP signal of OsTKPR1-GFP was mainly observed in ER-like structures (Fig. 7d). The ER localization of OsTKPR1 protein was further verified by Agrobacterium mediated co-transformation, which showed OsTKPR1-GFP signal overlapping with that of the ER-marker CD3–959 [39] tagged with mCherry (Fig. 7f).

Fig. 7
figure 7

Confocal images showing the subcellular localization of the OsTKPR1-GFP protein in tobacco leaf epidermal cells. Images taken from the GFP fluorescence (Green; a and d), bright field (b), mcherry fluorescence (Red; e) and the merged image (c and f). a-c Localization of the control GFP protein in the nucleus and cytoplasm. d and f Localization of the OsTKPR1-GFP protein in the ER. e Co-localization of OsTKPR1-GFP with the ER-marker of mcherry. Bars = 50 μm

Full size image

Reduced cuticular lipid components in ostkpr1–2 anthers

The abnormal anther cuticle and pollen exine in ostkpr1–2 anthers suggested the mutant may be defective in the biosynthesis of lipidic precursors for sporopollenin and cutin. To assess this, waxes and cutin monomers in both WT and ostkpr1–2 anthers at stage 12 were measured by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) as previously described [28]. The results indicated that the levels of both wax and cutin monomers were significantly reduced in ostkpr1–2 anthers as compared with those of the WT (Fig. 8a). The total amount of wax measured in ostkpr1–2 was 0.061 μg/mm2, a reduction of approximately 30% compared to WT (0.093 μg/mm2) (Fig. 8a). This reduction in total wax content in ostkpr1–2 anthers was attributed mainly to a significant reduction of the major rice anther wax components, such as fatty acids and saturated alkanes, although amounts of alkenes (C29:1, C31:1, C35:1) and sterols (campesterol and stigmasterol) were increased (Fig. 8c; Additional file 5: Table S2). The total amount of cutin monomers detected in WT was 0.372 μg/mm2, which also reflected a 30% reduction of the levels detected in ostkpr1–2 (0.265 μg/mm2) (Fig. 8a). The reduction of cutin monomers was due to a significant reduction in the levels of fatty acids and ω-hydroxylated fatty acids, although levels of other 2-hydroxylated fatty acids (2HFAs, including length of C21:0, C22:0, C24:0, C25:0) and ferulic acid were increased (Fig. 8b; Additional file 6: Table S3). Given that the rice tapetum utilizes a common biosynthetic pathway to provide precursors of the anther cuticle and sporopollenin [6, 10, 40], our chemical analysis suggests that OsTKPR1–2 plays an essential role in synthesizing lipidic precursors for the formation of both the anther cuticle and pollen exine in rice.

Fig. 8
figure 8

Analysis of anther wax and cutin in WT and ostkpr1–2. a Total anther cutin and wax content. b Profile of anther cutin monomers c Profile of anther wax constituents. The results are expressed as mean ± standard derivation of four biological replicates. Compound names are abbreviated as follows: C16:0 FA, palmitic acid; C18:0 FA, stearic acid; C18:1 FA, oleic acid; C18:2 FA, linoleic acid; C18:3 FA, linolenic acid; C20:0 FA, eicosanoic acid; C20:1 FA, gadoleic acid; C22:0 FA, docosanoic acid; C16:0 ω-HFA, 16-hydroxy-hexadecanoic acid; C18:1 ω-HFA, 18-hydroxy-oleic acid; C18:2 ω-HFA, 18-hydroxy-linoleic acid; C18:2 ω-HFA(2), 18-hydroxy-linoleic acid(2); cis-9,10-epoxy C18:0 ω-HFA, cis-9,10-epoxy 18-hydroxy-stearic acid; cis-9,10-epoxy C18:1 ω-HFA, cis-9,10-epoxy 18-hydroxyoleic acid; C26 FA, cerotic acid; ALK, alkane; C20:2 ω-HFA, 20-hydroxy-eicosadienoic acid; 9,10,16 Tri-OH C16 FA, 9,10,16-hydroxy-hexadecanoic acid; C20:0 2HFA, 2-hydroxyeicosanoic acid; C21:0 2HFA, 2-hydroxyheneicosanoic acid; C22:0 2HFA, 2-hydroxydocosanoic acid; C24:0 2HFA, 2-hydroxytetracosanoic acid; C25:0 2HFA, 2-hydroxypentacosenoic acid; ALK, alkane. UI, unknown chemical. *P < 0.05; **P < 0.01 (Student’s t test)

Full size image

Altered phenylpropanoid metabolism in ostkpr1–2 anthers

Previous studies reported that components of sporopollenin metabolon, such as PKSB/LAP5 and OsPKS2, are also involved in phenylpropanoid metabolism, contributing phenolic precursors for pollen wall formation [16, 28]. To investigate whether the loss of OsTKPR1 function also has effects on phenylpropanoid metabolism, phenolic profiles were generated for extracts of stage 11 anthers from wild type and ostkpr1–2 using an untargeted UHPLC-MS/MS approach. This analysis identified 238 metabolites in total; among them, 67 were flavonoids and 22 were hydroxycinnamyl derivatives.

When focusing on the phenylpropanoid pathway, levels of phenylalanine (the precursor for phenylpropanoids), naringenin chalcone and naringenin (important intermediate metabolites in the phenylpropanoid pathway), kaempferol-3-Oglucoside and tricin-O-hexoside-deoxyhexoside (sugar-conjugated derivatives of downstream metabolites) were decreased in mutant anthers. In contrast, levels of luteolin and dihydroquercetin (sugar-conjugated derivatives of downstream metabolites of apigenin and quercetin, respectively) were significantly increased in ostkpr1–2 mutant anthers (Fig. 9). The inhibitory effect of OsTKPR1 on the anther phenylpropanoid pathway is distinct from that observed in OsPKS2 [28], but similar to that of PKSB/LAP5 or PKSA/LAP6 [16], suggesting that disruption of individual sporopollenin metabolon components might have different effects on phenylpropanoid metabolism in flowering plants.

Fig. 9
figure 9

Analysis of the phenylpropanoid pathway in WT and ostkpr1–2 anthers. Red and Green colors denote increased and decreased abundance of compounds. PAL, phenylalanine ammonia-lyase; C4H, cinnamic acid 4-hydroxylase; 4CL, 4-coumarate ligase; CHS, chalconesynthase; CHI, chalcone isomerase; F3H, flavanone 3-hydroxylase; F3’H, flavonoid 3′-hydroxylase; FLS, flavonol synthase; FNS, flavone synthase. Numbers in the bracket show the altered fold changes of corresponding compounds. The # symbol in some compounds means isomerides

Full size image

Discussion

In Arabidopsis, the “sporopollenin metabolon” consists of ACOS5-PKSA/B-TKPR1 and plays essential roles in pollen wall formation [32]. Although this ancient metabolon is found to be present in many plants, including tobacco and rice [27], increasing evidence highlights both conserved and diversified functions of each component between dicots and monocots [12, 28, 30, 37]. In this study, we characterized OsTKPR1, the orthologue of Arabidopsis TKPR1, and revealed its vital roles in male reproduction in rice. Our results confirm the conserved function of OsTKPR1 in pollen wall formation [27] and highlight potentially additional functions in tapetum PCD, anther cuticle patterning and Ubisch body formation.

OsTKPR1–2 is indispensable for anther cuticle development and pollen exine formation

The anther-specific TKPR1 gene in Arabidopsis was first thought to be involved in flavonoid pathway, but was later found to act downstream of PKS, catalyzing the reduction of the carbonyl group of tetraketides generated by PKS to secondary alcohols, thus functioning in sporopollenin formation [27, 31]. In Arabidopsis, TKPR1 is important for male fertility, and the tkpr1 mutant displays a completely disorganized thin exine without bacula and tectum but a normal anther surface [31, 41]. In rice, OsTKPR1 exhibits the same enzyme activity as tobacco TKPR1 proteins, participating in the reduction of tetraketides, and ostkpr1 mutants produce no mature pollen or seeds (Additional file 2: Figure S1) [27]. In this study we found that in addition to defective exine (Fig. 4g, h), ostkpr1–2 mutants also display defective anther cuticle and Ubisch bodies (Fig. 3b, d; Fig. 4f), indicating that OsTKPR1 may contribute to the formation of the anther surface and pollen wall. Although stage 9 and 10 anthers were not directly analyzed due to difficulties in tissue collection, chemical analyses of stage 11 anthers were consistent with cytological observations, which revealed that levels of anther cuticular lipids (Fig. 8) and anther phenolic profiles were significantly altered in mutants (Fig. 9). These results are consistent with previous findings in rice that both the anther cuticle and pollen exine share common lipid and phenolic metabolic pathways [6, 20, 40]. The spatio-temporal expression pattern of OsTKPR1 in the tapetum and microspores (Fig. 6 b, c), and its similar ER localization (Fig. 7) to that of TKPR1 or NtTKPR1 [27, 31] provide further support for a role of OsTKPR1 in both anther and pollen development.

OsTKPR1–2 is possibly involved in tapetum degradation

The successful formation of anther cuticle and pollen wall depends largely on the timely degradation of the tapetum, and disruption of tapetum degradation usually leads to male sterility with defective anther cuticle and pollen wall [10,11,12, 21, 25, 42]. Although the function of TKPR1 on tapetum degradation is not reported in Arabidopsis or tobacco [27, 31], our data suggests that OsTKPR1 contributes to tapetum degradation in rice (Fig. 2), which is consistent with the spatial and temporal expression pattern of OsTKPR1 mRNA (Fig. 6) in the initial stage of tapetum degradation [4]. Additional evidence suggests that other components of the sporopollenin metabolon may impact tapetum PCD in rice. For example, a recent study revealed the role of OsACOS12 in tapetum PCD by both cytological and TUNEL analyses [12], which is clearly different from its ortholog ACOS5 in Arabidopsis [26] or NtACOS1 in tobacco [27]. Conversely, there is no report of the effect of OsPKS2 or OsPKS1 on tapetum PCD in rice [28,29,30].

The mechanism underlying OsTKPR1’s function on tapetum degradation was not investigated in this study. It is possible that OsTKPR1 fulfils a similar role in Arabidopsis and tobacco, but it has been overlooked in previous studies; alternatively, the role of sporopollenin metabolon components in tapetum PCD might be more prominent in monocots. Nevertheless, without additional evidence, it is also possible that the delayed tapetum degradation in ostkpr1–2 is an indirect effect.

Diverse interactions between sporopollenin metabolon components influence rice male fertility

In the sporopollenin metabolon, fatty acids synthesized de novo in plastids are modified by CYP703A2/OsCYP703A3 [18, 21] to produce hydroxylated fatty acids, which are then activated by ACOS (ACOS5/OsACOS12/NtACOS1) to generate fatty acyl CoAs [12, 26]. Fatty acyl CoAs are condensed first by PKS (PKSA/LAP6/OsPKS1 and PKSB/LAP5/NtPKS1/OsPKS2) [16, 17, 28], and the resultant triketides and tetraketides (also called α-pyrones) are then reduced by TKPR (TKPR1/OsTKPR1/NtTKPR1) to produce sporopollenin precursors, such as hydroxylated tetraketides and secondary alcohols [27, 31]. In Arabidopsis, ACOS5 directly interacts with PKSA, PKSB and TKPR1 in the ER of the tapetum to form a multi-enzyme association [32]. ACOS5 also interacts with CYP703A2 [32], a conserved enzyme involved in the biosynthesis of precursors for anther cuticle and pollen exine formation [18]. In Brassica napus, BnPKSB directly interacts with BnPKA and BnACOS5, but does not interacts with BnTKPR [35]. Notably, the interaction between BnPKSA and BnPKSB has not been reported for their orthologues in Arabidopsis. Similarly, interactions among these individual metabolon components have not been reported in rice and tobacco [29]. Although further studies are required, these differences may indicate there is a degree of flexibility in the interactions between different metabolon components in different plant systems.

Genetic evidence also suggests a degree of specialization in the role of metabolon components. The acos5, osacos12 and ntacos1 mutants all produce pollen that apparently lack exine [12, 26, 27, 36], but unlike acos5 and ntacos1, osacos12 displays additional defects including smooth anthers with a reduced anther cuticle [12, 36], abnormal Ubisch bodies and delayed tapetum degradation [12]. Expression of OsACOS12 under the control of the ACOS5 promoter partially restores male fertility of acos5 mutants [36], suggesting at least some degree of functional conservation. In addition, while pksa/lap6 and pksb/lap5 single mutants produce fertile pollen with irregular exine patterning, the double mutant is completely male sterile with pollen lacking exine deposition [16, 17]. By contrast, tobacco plants expressing NtPKS1-RNAi [27] and the single mutant of ospks2 [28, 30] are complete sterile, producing aborted pollen with disorganized exine and abnormal anther surfaces. OsPKS2 driven by the PKSB/LAP5 promoter can partially rescue the abnormal exine patterning of pksb/lap5 [28].

Additional evidence supporting functional diversity is derived from the in vitro activity of metabolon components. ACOS belongs to the 4-coumarate CoA ligase (4CL) family, however, in vitro enzyme assays indicate that ACOS5, NtACOS1 and OsACOS12 are not 4CL, but rather fatty acyl-CoA synthetases. The preferential substrates of these enzymes are hydroxylated fatty acids (C8 to C18), fatty acids (C10 to C16) and fatty acid (C18:1), respectively [12, 26, 27, 43]. Plant specific type III PKS is thought to have chalcone synthase (CHS) activity, but an in vitro CHS activity test confirms that PKSA/B (LAP6/5), NtPKS1 and OsPKS1 are not CHS enzymes, but rather are true polyketide synthases. These enzymes have preferential substrates of fatty acyl-CoAs with different carbon length, C6 to C12, C10 to C16, and C16 to C18, respectively, generating corresponding α-pyrone lipids [16, 27, 28]. Although ACOS and PKS in Arabidopsis, tobacco and rice have similar functions, they differ clearly in substrate preference. Unfortunately, in vivo activity of ACOS5/NtACOS1/OsAOC12 has yet to be reported and metabolic analysis failed to identify pyrenes in developing anthers [16], meaning that in planta substrates of ACOS and PKS remain to be determined. Previous studies revealed that neither NtTKPR1 nor OsTKPR1 has DFR activity, because typical substrates including dihydrokaempferol and dihydroquercetin can’t be metabolized by NtTKPR1 or OsTKPR1 [27]. However, the significant accumulation of dihydroquercetin in ostkpr1–2 anthers (Fig. 9) could be a clue that OsTKPR1 has in vivo DFR activity in rice. Alternatively, given the significant accumulation of several 2-hydroxylated fatty acids and ferulic acid in anther cutin profile (Fig. 8), it is plausible that both 2-hydroxylated fatty acids and ferulic acid could be the in vivo substrates of OsTKPR1. Our previous study implies that ferulic acid is important for the polymerization of anther cutin, and pollen sporopollenin [3]. Upon the loss of OsTKPR1 activity, the conjugates between aliphatic 2-hydroxylated fatty acids and aromatic ferulic acid in mutant anthers can’t be reduced to corresponding secondary alcohols, thus, after de-polymerization, corresponding individual monomers accumulate significantly. At this point in time we can’t provide additional evidence to distinguish above hypotheses, which merits further investigations in future studies.

The final evidence for distinct functions of metabolon components comes from the different effects of these proteins on phenylpropanoid metabolism. Results from this study and others [16, 28] suggest that phenylpropanoid metabolism is equally important as lipid metabolism in plant male fertility. The inhibition of flavonoid metabolism in ostkpr1–2 anthers was also reported in pksa/lap6 and pksb/lap5 mutants [16]. In contrast, flavonoid metabolism in ospks2 is significantly activated [28]. Therefore, although ostkpr1–2 is male sterile, similar to that of ospks2 [28], ostkpr1–2 showed a different pollen surface and anther inner surface to those of ospks2 [28] (Fig. 3), which may be the consequence of the different effects of these two proteins on the flavonoid pathway (Fig. 9) [28]. In addition, different proteins differentially impact ferulic acid metabolism. It is reported that the level of free aromatic lipid ferulic acid is increased in ospks2 [28], while the level of polymerized ferulic acid was found to be increased in ostkpr1–2 (Fig. 8 b). This difference could contribute to the different phenotypes observed in ospks2 and ostkpr1–2 (Fig. 4). Our previous study implies that ferulic acid is important for the polymerization of anther cutin, and pollen sporopollenin [3].

Taken together, our studies suggest that OsTKPR1 is a conserved sporopollenin metabolon component but with possibly unique biochemical functions important for male fertility in rice. Some of these unique functions could form the basis of future comparative studies of pollen development between monocot and dicot species.

Conclusions

Our functional characterization results suggested that OsTKPR1, an orthologue of Arabidopsis TKPR1, is indispensable for male fertility in rice. OsTKPR1 affected not only the formation of pollen but also the formation of anther cuticle, which provided new insights into the conserved sporopollenin metabolon in flowering plants.

Methods

Plant materials and molecular cloning of OsTKPR1–2

WT and ostkpr1–2 (9522 background, japonica) heterozygous rice seeds were sourced from our lab seeds stock while T-DNA insertion line PFG_2B-00257 (ostkpr1) heterozygous seeds were a gift from Professor Clive Lo (The University of Hong Kong). All rice plants, including wild-type (WT), ostkpr1, and ostkpr1–2 plants, were grown in the paddy field of Shanghai Jiao Tong University (31.03°N, 121.45°E), Shanghai, China. Map-based cloning was performed as previously described [44] with F2 populations generated from crosses between ostkpr1–2 and Guang Lu Ai 4 (indica). All primers used for mapping are listed in Additional file 7: Table S4.

Phenotypic observation

Images of the whole plant and reproductive organs were captured with a Nikon E995 digital camera and a Leica M205A microscope, respectively. Routine analysis of I2-KI staining, semi-thin cross sections, SEM (scanning electron microscopy) and TEM (transmission electron microscopy) were performed as described previously [3, 10]. The classification of various stages of developing rice anthers were carried out as described [4].

Quantitative RT-PCR analysis

Total RNA was isolated from various rice tissues using Trizol reagent (Tiangen, Beijing, China), and analyzed with a Nanodrop 1000 spectrophotometer (Thermo Scientific, UT) for assessment of quality and quantity. For RT-PCR, first-strand cDNA was reverse transcribed from total RNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time; TaKaRa). Real-time RT-PCR was performed with iQ SYBR Green Supermix (Bio-rad), using the real-time PCR system (Bio-Rad C1000 CFX96). The rice actin gene was used as an internal control. Primers used to quantify the expression of OsTKPR1 are listed in Additional file 7: Table S4.

In situ hybridization

RNA hybridization and immunological detection of the hybridized probes were performed according to the protocol as previously [45]. The primers used for generation of in situ hybridization probes are listed in Additional file 7: Table S4.

Subcellular localization of OsTKPR1

For transient expression in tobacco (Nicotiana benthamiana) leaves, full-length OsTKPR1 CDS was PCR amplified using OsTKPR1CDS-F and OsTKPR1CDS-R (Additional file 7: Table S4), and introduced into the 1301-GFP vector digested with Bgl II and Spe I to generate 1301-35Spro:OsTKPR1CDS-GFP. The GFP construct was transformed into Agrobacterium strain GV3101 and infiltrated into leaves of 4-week-old tobacco plants. After two day’s dark culture, the tobacco leaf epidermis with 1301-35Spro:OsTKPR1CDS-GFP was mounted in water and observed under a confocal microscope (Leica TCS SP5).

Analysis of anther wax and cutin

Anther wax and cutin were analyzed as described previously [3]. The measurement of the anther surface was carried out by plotting the calculated anther surface area against anther dry weight as described previously (Additional file 8: Figure S4) [20].

Metabolite profiling and data analysis

Four biological replications of rice anthers at stage 11 were collected, immediately frozen in liquid nitrogen, and lyophilized for metabolomics analysis. Methanol extracts from 10 mg of anther powder per sample were analyzed by UHPLC-MS/MS as described previously [46]. Metabolite identification and data analysis were performed as described previously [46].

Abbreviations

GC-FID:

gas chromatography-flame ionization detection

GC-MS:

gas chromatography-mass spectrometry

PCD:

programmed cell death

SEM:

scanning electron microscopy

TEM:

transmission electron microscopy

References

  1. Ariizumi T, Toriyama K. Genetic regulation of sporopollenin synthesis and pollen exine development. Annu Rev Plant Biol. 2011;62:437–60.

    Article  CAS  Google Scholar 

  2. Scott RJ, Spielman M, Dickinson HG. Stamen structure and function. Plant Cell. 2004;16(suppl 1):S46–60.

    Article  CAS  Google Scholar 

  3. Xu D, Shi J, Rautengarten C, Yang L, Qian X, Uzair M, et al. Defective Pollen Wall 2 (DPW2) encodes an acyl transferase required for rice pollen development. Plant Physiol. 2017;173(1):240–55.

    Article  CAS  Google Scholar 

  4. Zhang D, Luo X, Zhu L. Cytological analysis and genetic control of rice anther development. J Genet Genomics. 2011;38(9):379–90.

    Article  CAS  Google Scholar 

  5. Quilichini TD, Grienenberger E, Douglas CJ. The biosynthesis, composition and assembly of the outer pollen wall: a tough case to crack. Phytochemistry. 2015;113:170–82.

    Article  CAS  Google Scholar 

  6. Shi J, Cui M, Yang L, Kim Y-J, Zhang D. Genetic and biochemical mechanisms of pollen wall development. Trends Plant Sci. 2015;20:741–53.

    Article  CAS  Google Scholar 

  7. Ahlers F, Thom I, Lambert J, Kuckuk R, Wiermann R. 1H NMR analysis of sporopollenin from Typha Angustifolia. Phytochemistry. 1999;50(6):1095–8.

    Article  CAS  Google Scholar 

  8. Piffanelli P, Ross JH, Murphy D. Biogenesis and function of the lipidic structures of pollen grains. Sex Plant Reprod. 1998;11(2):65–80.

    Article  CAS  Google Scholar 

  9. Scott RJ. Pollen exine-the sporopollenin enigma and the physics of pattern. In: Scott RJ, Stead AD, editors. Molecular and cellular aspects of plant reproduction. Cambridge, UK: Cambridge University Press; 1994. p. 49–81.

    Chapter  Google Scholar 

  10. Li N, Zhang D, Liu H, Yin C, Li X, Liang W, et al. The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. Plant Cell. 2006;18(11):2999–3014.

    Article  CAS  Google Scholar 

  11. Xu J, Yang C, Yuan Z, Zhang D, Gondwe MY, Ding Z, Liang W, Zhang D, Wilson ZA. The ABORTED MICROSPORES regulatory network is required for postmeiotic male reproductive development in Arabidopsis thaliana. Plant Cell. 2010;22(1):91–107.

    Article  CAS  Google Scholar 

  12. Yang X, Liang W, Chen M, Zhang D, Zhao X, Shi J. Rice fatty acyl-CoA synthetase OsACOS12 is required for tapetum programmed cell death and male fertility. Planta. 2017;246(1):105–22.

    Article  CAS  Google Scholar 

  13. Jiang J, Zhang Z, Cao J. Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol. 2013;15(2):249–63.

    Article  CAS  Google Scholar 

  14. Domínguez E, Mercado JA, Quesada MA, Heredia A. Pollen sporopollenin: degradation and structural elucidation. Sex Plant Reprod. 1999;12(3):171–8.

    Article  Google Scholar 

  15. Wellman CH. Origin, function and development of the spore wall in early land plants. In: Hemsley AR, Poole I, editors. The evolution of plant physiology: from whole plants to ecosystems. London, UK: Elsevier; 2004. p. 43–63.

    Chapter  Google Scholar 

  16. Dobritsa AA, Lei Z, Nishikawa S, Urbanczyk-Wochniak E, Huhman DV, Preuss D, Sumner LW. LAP5 and LAP6 encode anther-specific proteins with similarity to chalcone synthase essential for pollen exine development in Arabidopsis thaliana. Plant Physiol. 2010;153:937–95.

    Article  CAS  Google Scholar 

  17. Kim SS, Grienenberger E, Lallemand B, Colpitts CC, Kim SY, de Azevedo SC, Geoffroy P, Heintz D, Krahn D, Kaiser M. LAP6/POLYKETIDE SYNTHASE a and LAP5/POLYKETIDE SYNTHASE B encode hydroxyalkyl α-pyrone synthases required for pollen development and sporopollenin biosynthesis in Arabidopsis thaliana. Plant Cell. 2010;22(12):4045–66.

    Article  CAS  Google Scholar 

  18. Morant M, Jørgensen K, Schaller H, Pinot F, Møller BL, Werck-Reichhart D, Bak S. CYP703 is an ancient cytochrome P450 in land plants catalyzing in-chain hydroxylation of lauric acid to provide building blocks for sporopollenin synthesis in pollen. Plant Cell. 2007;19(5):1473–87.

    Article  CAS  Google Scholar 

  19. Chen W, Yu X, Zhang K, Shi J, De Oliveira S, Schreiber L, Shanklin J, Zhang D. Male Sterile2 encodes a plastid-localized fatty acyl carrier protein reductase required for pollen exine development in Arabidopsis. Plant Physiol. 2011;157(2):842–53.

    Article  CAS  Google Scholar 

  20. Shi J, Tan HX, Yu XH, Liu YY, Liang WQ, Ranathunge K, et al. Defective Pollen Wall is required for anther and microspore development in rice and encodes a fatty acyl carrier protein reductase. Plant Cell. 2011;23(6):2225–46.

    Article  CAS  Google Scholar 

  21. Yang X, Wu D, Shi J, He Y, Pinot F, Grausem B, Yin C, Zhu L, Chen M, Luo Z. Rice CYP703A3, a cytochrome P450 hydroxylase, is essential for development of anther cuticle and pollen exine. J Integr Plant Biol. 2014;56(10):979–94.

    Article  CAS  Google Scholar 

  22. Dobritsa AA, Shrestha J, Morant M, Pinot F, Matsuno M, Swanson R, Moller BL, Preuss D. CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis. Plant Physiol. 2009;151:574–89.

    Article  CAS  Google Scholar 

  23. Li H, Piont F, Sauveplane V, Werck-Reichhart D, Diehl P, Schreiber L, et al. Cytochrome P450 family member CYP704B2 catalyzes the ω-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice. Plant Cell. 2010;22:173–90.

    Article  CAS  Google Scholar 

  24. Choi H, Jin JY, Choi S, Hwang JU, Kim YY, Suh MC, Lee Y. An ABCG/WBC-type ABC transporter is essential for transport of sporopollenin precursors for exine formation in developing pollen. Plant J. 2011;65(2):181–93.

    Article  CAS  Google Scholar 

  25. Zhu L, Shi J, Zhao G, Zhang D, Liang W. Post-meiotic deficient anther1 (PDA1) encodes an ABC transporter required for the development of anther cuticle and pollen exine in rice. J Plant Biol. 2013;56:59–68.

    Article  CAS  Google Scholar 

  26. de Azevedo SC, Kim SS, Koch S, Kienow L, Schneider K, McKim SM, Haughn GW, Kombrink E, Douglas CJ. A novel fatty acyl-CoA synthetase is required for pollen development and sporopollenin biosynthesis in Arabidopsis. Plant Cell. 2009;21(2):507–25.

    Article  Google Scholar 

  27. Wang Y, Lin YC, So J, Du Y, Lo C. Conserved metabolic steps for sporopollenin precursor formation in tobacco and rice. Physiol Plantarum. 2013;149(1):13–24.

    Article  CAS  Google Scholar 

  28. Zhu X, Yu J, Shi J, Tohge T, Fernie AR, Meir S, et al. The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice. J Integr Plant Biol. 2017;59:612–28.

    Article  CAS  Google Scholar 

  29. Zou T, Xiao Q, Li W, Luo T, Yuan G, He Z, et al. OsLAP6/OsPKS1, an orthologue of Arabidopsis PKSA/LAP6, is critical for proper pollen exine formation. Rice. 2017;10:53.

    Article  Google Scholar 

  30. Zou T, Liu M, Xiao Q, Wang T, Chen D, Luo T, et al. OsPKS2 is required for rice male fertility by participating in pollen wall formation. Plant Cell Rep. 2018;37(5):759–73.

    Article  CAS  Google Scholar 

  31. Grienenberger E, Kim SS, Lallemand B, Geoffroy P, Heintz D, de Azevedo SC, Heitz T, Douglas CJ, Legrand M. Analysis of TETRAKETIDE α-PYRONE REDUCTASE function in Arabidopsis thaliana reveals a previously unknown, but conserved, biochemical pathway in sporopollenin monomer biosynthesis. Plant Cell. 2010;22(12):4067–83.

    Article  CAS  Google Scholar 

  32. Lallemand B, Erhardt M, Heitz T, Legrand M. Sporopollenin biosynthetic enzymes interact and constitute a metabolon localized to the endoplasmic reticulum of tapetum cells. Plant Physiol. 2013;162:615–25.

    Article  Google Scholar 

  33. Colpitts CC, Kim SS, Posehn SE, Jepson C, Kim SY, Wiedemann G, Reski R, Wee AG, Douglas CJ, Suh DY. PpASCL, a moss ortholog of anther-specific chalcone synthase-like enzymes, is a hydroxyalkylpyrone synthase involved in an evolutionarily conserved sporopollenin biosynthesis pathway. New Phytol. 2011;192(4):855–68.

    Article  CAS  Google Scholar 

  34. Jepson C, Karppinen K, Daku RM, Sterenberg BT, Suh DY. Hypericum perforatum hydroxyalkylpyrone synthase involved in sporopollenin biosynthesis-phylogeny, site-directed mutagenesis, and expression in nonanther tissues. FEBS J. 2014;281(17):3855–68.

    Article  CAS  Google Scholar 

  35. Qin M, Tian T, Xia S, Wang Z, Song L, Yi B, et al. Heterodimer formation of BnPKSA or BnPKSB with BnACOS5 constitutes a multienzyme complex in tapetal cells and is involved in male reproductive development in Brassica napus. Plant Cell Physiol. 2016;57(8):1643–56.

    Article  CAS  Google Scholar 

  36. Li Y, Li D, Guo Z, Shi Q, Xiong S, Zhang C, Zhu J, Yang Z. OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice. BMC Plant Biol. 2016; 16(1):256.DOI https://doi.org/10.1186/s12870-016-0943-9

  37. Zou T, He Z, Qu L, Liu M, Zeng J, Liang Y, et al. Knockout of OsACOS12 caused male sterility in rice. Mol Breeding. 2017;37(10):126.

    Article  Google Scholar 

  38. Yau CP, Zhuang CX, Zee SY, Yip WK. Expression of a microsporocyte-specific gene encoding dihydroflavonol 4-reductase-like protein is developmentally regulated during early microsporogenesis in rice. Sex Plant Reprod. 2005;18(2):65–74.

    Article  CAS  Google Scholar 

  39. Nelson BK, Cai X, Nebenführ A. A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant J. 2007;51(6):1126–36.

    Article  CAS  Google Scholar 

  40. Li H, Zhang D. Biosynthesis of anther cuticle and pollen exine in rice. Plant Signal Behav. 2010;5(9):1121–3.

    Article  CAS  Google Scholar 

  41. Tang LK, Chu H, Yip WK, Yeung EC, Lo C. An anther-specific dihydroflavonol 4-reductase-like gene (DRL1) is essential for male fertility in Arabidopsis. New Phytol. 2009;181(3):576–87.

    Article  CAS  Google Scholar 

  42. Zhao G, Shi J, Liang W, Xue F, Luo Q, Zhu L, et al. Two ATP binding cassette G transporters, rice ATP binding cassette G26 and ATP binding cassette G15, collaboratively regulate rice male reproduction. Plant Physiol. 2015;169(3):2064–79.

    CAS  PubMed  PubMed Central  Google Scholar 

  43. Kienow L, Schneider K, Bartsch M, Stuible H, Weng H, Miersch O, Wasternack C, Kombrink E. Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme a synthetase family from Arabidopsis thaliana. J Experimental Botany. 2008;59(2):403–19.

    Article  CAS  Google Scholar 

  44. Chu H, Liu H, Li H, Wang H, Wei J, Li N, et al. Genetic analysis and mapping of the rice leafy-hull mutant oslh. J Plant Physiol Mol Biol. 2005;31(6):594–8.

    CAS  Google Scholar 

  45. Kouchi H, Hata S. Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development. Mol Gen Genet. 1993;238(1–2):106–19.

    CAS  PubMed  Google Scholar 

  46. Hu C, Tohge T, Chan S, Song Y, Rao J, Cui B, et al. Identification of conserved and diverse metabolic shifts during rice grain development. Sci Rep. 2016. https://doi.org/10.1038/srep20942.

Download references

Acknowledgements

The authors would like to thank Dr. Guorun Qu and Qian Luo for their assistance in anther cuticle analysis; we thank Zhijin Luo and Mingjiao Chen for mutant screening and generation of F2 populations for the mapping.

Funding

This research was supported by grants from China Postdoctoral Science Foundation (2017 M611545); the National Natural Science Foundation of China (Grants 31430009, 31322040, and 31271698); the National Key Research and Development Program of China (Grants 2016YFD0100804 and 2016YFE0101000); National Key Basic Research Development Program of the Ministry of Science and Technology, China (Grant 2013 CB126902); the Innovative Research Team, Ministry of Education, and 111 Project (Grant B14016). The funding organizations played no role in the design of study, choice of enrolled patients, review and interpretation of data, or preparation or approval of manuscript.

Availability of data and materials

All data generated or analyzed during this study are included in this published article and its supplementary information files. The gene sequence of OsTKPR1 is deposited in the Phytozome 12 (https://phytozome.jgi.doe.gov/pz/portal.html) under accession number LOC_Os09g32020.

Author information

Authors and Affiliations

  1. Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Dawei Xu, Shuying Qu, Dabing Zhang, Wanqi Liang & Jianxin Shi

  2. Flow Station of Post-doctoral Scientific Research, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Dawei Xu

  3. School of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, 5064, Australia

    Matthew R. Tucker & Dabing Zhang

Authors
  1. Dawei Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuying Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Matthew R. Tucker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dabing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wanqi Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jianxin Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

DWX, WQL, DBZ and JXS were involved in the design of the experiments and analyzed the data. DWX performed the experiments. SYQ helped for the map based coning of the mutant. DWX, MRT and JXS wrote the manuscript. All authors have read, edited and approved publication of the present paper.

Corresponding author

Correspondence to Jianxin Shi.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Additional files

Additional file 1:

Table S1. Allelism test of ostkpr1–2 with ostkpr1. (DOCX 22 kb)

Additional file 2:

Figure S1. Phenotypic comparison between WT and ostkpr1 T-DNA insertion mutant. a Plants after bolting. b Spikeltes after removal of the lemma and palea. c WT pollen grains stained with 2% I2-KI solution. d Stage 13 pollen grains of ostkpr1 stained with 2% I2-KI solution showing no pollen grains. e-j SEM observation for the WT (e, g, i) and ostkpr1 (f, h, j) anthers and pollens. e, f The epidermal surface of WT (e) and ostkpr1 (f) anthers. g, h SEM observation for the WT (g) and ostkpr1 (h) pollen grains. i, j The enlarged view of the surface of WT (i) and ostkpr1 pollen grains. Bars = 1 mm in b, 200 μm in c, d, 10 μm in e, f, 20 μm in g, h and 5 μm in i, j. (JPG 993 kb)

Additional file 3:

Figure S2. Spatio-temporal expression of OsTKPR1 in rice grown in the field. Data were obtained from an online microarray database http://ricexpro.dna.affrc.go.jp/. (JPG 326 kb)

Additional file 4:

Figure S3. Amino acid sequences alignment of OsTKPR1 and AtTKPR1. Sequences were aligned using Clustal W. (DOCX 45 kb)

Additional file 5:

Table S2. Detailed wax constituents in WT and ostkpr1–2 anthers. (DOCX 18 kb)

Additional file 6:

Table S3. Detailed cutin composition of the WT and ostkpr1–2 anthers. (DOCX 17 kb)

Additional file 7:

Table S4. Primers used in this work. (DOCX 17 kb)

Additional file 8:

Figure S4. Weight/Surface area ratio of WT and ostkpr1–2 anthers. The weight/surface area ratio of the anthers in the WT (blue squares) and ostkpr1–2 (red squares). (JPG 49 kb)

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xu, D., Qu, S., Tucker, M.R. et al. Ostkpr1 functions in anther cuticle development and pollen wall formation in rice. BMC Plant Biol 19, 104 (2019). https://doi.org/10.1186/s12870-019-1711-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12870-019-1711-4

Keywords

  • Anther cuticle
  • Oryza sativa
  • OsTKPR1
  • Pollen exine
  • Sporopollenin

BMC Plant Biology

ISSN: 1471-2229

Contact us