- Research article
- Open Access
Silencing of DND1 in potato and tomato impedes conidial germination, attachment and hyphal growth of Botrytis cinerea
BMC Plant Biology volume 17, Article number: 235 (2017)
Botrytis cinerea, a necrotrophic pathogenic fungus, attacks many crops including potato and tomato. Major genes for complete resistance to B. cinerea are not known in plants, but a few quantitative trait loci have been described in tomato. Loss of function of particular susceptibility (S) genes appears to provide a new source of resistance to B. cinerea in Arabidopsis.
In this study, orthologs of Arabidopsis S genes (DND1, DMR6, DMR1 and PMR4) were silenced by RNAi in potato and tomato (only for DND1). DND1 well-silenced potato and tomato plants showed significantly reduced diameters of B. cinerea lesions as compared to control plants, at all-time points analysed. Reduced lesion diameter was also observed on leaves of DMR6 silenced potato plants but only at 3 days post inoculation (dpi). The DMR1 and PMR4 silenced potato transformants were as susceptible as the control cv Desiree. Microscopic analysis was performed to observe B. cinerea infection progress in DND1 well-silenced potato and tomato leaves. A significantly lower number of B. cinerea conidia remained attached to the leaf surface of DND1 well-silenced potato and tomato plants and the hyphal growth of germlings was hampered.
This is the first report of a cytological investigation of Botrytis development on DND1-silenced crop plants. Silencing of DND1 led to reduced susceptibility to Botrytis, which was associated with impediment of conidial germination and attachment as well as hyphal growth. Our results provide new insights regarding the use of S genes in resistance breeding.
Botrytis cinerea, a necrotrophic pathogen which causes grey mold disease, can infect a broad range of plant species including potato and tomato. Every year, global expenses of Botrytis control surmount €1 billion . Hence, B. cinerea has become the most extensively studied necrotrophic fungal pathogen. Necrotrophic pathogens damage plant cells by secreting toxic compounds, lytic enzymes and an array of pathogenicity factors which can subdue host defences . In order to penetrate the plant cuticle and epidermal cells, B. cinerea produces cutin esterases, pectinases and hemicellulases during the early infection stages, between 8 and 16 h post inoculation (hpi) [3,4,5,6]. At later stages, between 16 and 40 hpi, multiple phytotoxic metabolites such as botrydial, botcinic acid and oxalic acid, as well as several phytotoxic proteins are produced and secreted into epidermal and mesophyll tissue to kill the host cells [2, 6].
The infection process in susceptible potato and tomato leaf tissue starts when conidia land on the leaf. Prior to germination, conidia adhere weakly to the plant surface by weak hydrophobic interactions [7, 8]. When inoculated in nutrient solution, conidia generally germinate within 3–4 h and form one or two germ tubes that can elongate to 20–40 μm, and are covered with a polysaccharide glucan matrix [9, 10] that increases the adhesion to the plant surface. Around 6 hpi, the tip of the germ tube develops a swollen, non-melanized appressorium  that is tightly attached to the plant surface by a mucilage. In the absence of melanin, the appressorium does not exert high physical pressure, but it uses hydrolytic enzymes to penetrate the plant cuticle around 8 hpi. Fungal hyphae subsequently grow inside host tissue over a limited distance, with no visible signs of host cell death until ~14–16 hpi. The infection zone may at this time point develop water-soaked pinpoint lesions that result from degradation of host cell walls, however, the infection droplet is colourless. From 16 to 18 hpi onwards, plant cells begin to collapse and turn necrotic, and the infection droplet turns in colour by the accumulation of brown (phenolic) compounds . Typical scanning electron microscopy (SEM) images of the different stages of infection on broad bean are provided in Tenberge (2007) .
No major genes (R genes) for complete resistance to B. cinerea have been identified in plants. However, quantitative trait loci (QTLs) reducing the outgrowth of B. cinerea have been reported in several plant species, first in Arabidopsis . In the wild tomato accession LYC4 from Solanum habrochaites, ten major QTLs have been described for reduced lesion growth and for reduced disease incidence . More recently, QTLs have been identified for resistance to B. cinerea in chickpea and Brassica rapa [16, 17].
Recently, plant genes (susceptibility or S genes) that are recruited by pathogens to promote diseases have become the focal point of research attention . In Arabidopsis and tomato, 16 plant genes have been reported that participate in disease development by B. cinerea (Additional file 1: Table S1). Examples of these are the tomato genes, LePG and LeEXP1, which contribute to fruit cell wall softening during ripening and thereby facilitate penetration and colonization by B. cinerea , as well as the Arabidopsis CESA4, CESA7 and CESA8 genes, encoding subunits of the cellulose synthase complex .
Evidence is accumulating that loss-of-function mutations of certain plant S genes can result in resistance to pathogens [21,22,23,24,25]. In tomato, the ABA deficient mutant sitiens is highly resistant to B. cinerea [26,27,28]. In Arabidopsis, the invasion of B. cinerea was arrested in the bre1 mutant . The BRE1 gene, also known as the long-chain acyl-CoA synthetase2 (LACS2) gene, is essential for cutin biosynthesis. Furthermore, the Arabidopsis dnd1 (defense no death) mutant showed enhanced resistance against a range of fungal, bacterial and viral pathogens, including B. cinerea [30,31,32]. In our previous study, we reported that silencing of potato orthologs to DND1, DMR6, DMR1 and PMR4 resulted in resistance to late blight . These experiments demonstrated that plant S genes are conserved across plant species with respect to their function as a susceptibility factor to certain types of pathogens.
In this study, we aimed to test whether impairing the functionality of S genes in potato would confer resistance to B. cinerea. Potato RNAi transformants, in which orthologs of DND1, DMR6, DMR1 and PMR4 were silenced, were examined for reduced susceptibility to B. cinerea. In addition, we extended the analysis to DND1-silenced tomato plants and performed microscopy to follow the development of B. cinerea germlings on leaf surfaces.
StDND1 well-silenced potato plants show reduced susceptibility to Botrytis cinerea
In potato, silencing of the genes DND1, DMR1, DMR6 and PMR4 resulted in reduced susceptibility to late blight. In order to assess whether silencing these S gene candidates also leads to reduced susceptibility to B. cinerea, RNAi well-silenced transformants of StDMR1, StDMR6, StDND1 and StPMR4 were inoculated with the wild type strain B05.10 in a detached leaf assay (DLA). In all these transformants, except DND1A-6, the expression level of the corresponding target gene is significantly down-regulated (> 60% reduced S gene expression ). The leaves of cv Desiree were used as susceptible control. Lesion diameters on infected leaves were measured daily from 1 to 3 days post inoculation (dpi) (Fig. 1). Desiree and the DND1A-6(−) (RNAi weak-silenced transformant) showed similar B. cinerea lesions (Fig. 1). Compared to cv Desiree, significantly smaller B. cinerea lesions were observed on StDMR6 RNAi well-silenced transformants at 3 dpi and on StDND1 RNAi well-silenced transformants at all three time points (Fig. 1, Additional file 2: Figure S1 and Additional file 3: Figure S2).
B. cinerea conidial surface attachment and germling growth are reduced on leaves of StDND1 well-silenced potato plants
In order to get insight into the mechanism of reduced susceptibility, a histological study was performed on the fungal spore germination and outgrowth of germlings in B. cinerea-infected leaves of StDND1-silenced potato plants and of cv Desiree. A B. cinerea reporter strain was used that contains a GUS gene under control of the promoter of the cutinase gene BccutA, which is inactive in spores but induced from the onset of germination onwards . GUS activity and accumulation of H2O2 were monitored microscopically following histochemical staining and the number of spores per inoculation droplet was counted (Fig. 2). At 0.5 hpi, only few blue stained conidia could be observed on the leaf surfaces of all genotypes, partly because the BccutA promoter was at that moment inactive  and partly because conidia were washed from the leaf surface in the staining procedure due to their weak attachment to the cuticle . At 3 hpi, the majority of conidia had germinated on cv Desiree and the germ tubes increased in length at subsequent time points, 6 and 10 hpi. Both conidia and germ tubes stained blue due to the induction of the BccutA promoter during infection (Fig. 3). The total number of blue-stained conidia on the leaf surfaces at the three time points tested (3, 6, and 10 hpi) did not significantly differ between cv Desiree and the weakly-silenced plant DND1A-6 (Fig. 2). However, on the leaves of two StDND1 well-silenced plants the number of blue-stained conidia was two- to three-fold lower than on cv Desiree at all-time points analysed (Fig. 2). Fig. 3 shows that the blue GUS stain of fungal hyphae decreased to some extent at 10 hpi on cv Desiree, but vanished almost entirely in the StDND1 well-silenced potato plants (see also Additional file 4: Figure S3). DAB staining showed that H2O2 accumulated on the surface of fungal conidia and germ tubes (Fig. 3), as a result of the activity of glucose oxidase and superoxide dismutase, enzymes that are produced by B. cinerea and excreted to the fungus-host interface [35, 36]. The generation of H2O2 on the fungal matrix  is not harmful to B. cinerea and the fungus does not appear to experience intracellular oxidative stress during infection . In most cases, there was a correspondence between the GUS staining and DAB staining in fungal tissue, with one exception. Notably, at 10 hpi on StDND1 well-silenced potato plants, DAB staining detected clear accumulation of H2O2 on the fungal germ tube surfaces, even though fungal structures were barely detected following the GUS staining (Fig. 3). This observation suggested that fungal hyphae were damaged, possibly to such an extent that cytoplasmic content leaked out and GUS activity vanished from the hyphae, while H2O2 generating enzymes on the hyphal surface remained active.
At 24 hpi, necrotic lesions could be observed on the inoculation sites of cv Desiree leaves (Fig. 4a) which had the size of the initial inoculation droplet. Hyphae of B. cinerea could be observed that were extending below the plane of focus, indicating that hyphae might be underneath the leaf cuticle (Fig. 4b). On the leaves of StDND1 well-silenced plants, necrotic lesions were not visible (Fig. 4d) and only a small number of B. cinerea hyphae were observed that appeared to be growing mostly on the leaf surface and displayed very weak GUS staining (Fig. 4e). Accumulation of H2O2, as shown by dark DAB staining, was observed beyond the inoculation sites on cv Desiree plants (Fig. 4c). In contrast, DAB staining was limited to the inoculation sites on the StDND1 well-silenced plants and the intensity was less pronounced (Fig. 4f).
At 48 hpi, lesion development of B. cinerea was observed by naked eye on both cv Desiree and StDND1 well-silenced potato plants (Fig. 5, a and b). Necrotic spots, surrounded by a water-soaked lesion, developed on inoculated leaves of cv Desiree. Much smaller necrotic spots were observed on the StDND1 well-silenced potato plants and they were surrounded by chlorotic tissue rather than a water-soaked area typically observed in the control. Leaf samples of the inoculation sites were taken from both cv Desiree and StDND1 well-silenced potato plants after inoculation with B. cinerea or after mock-treatment (Potato Dextrose Broth, PDB) and were stained with GUS and DAB (Fig. 5c). GUS staining resulted in deep blue spots on leaves of cv Desiree, reflecting a dense growth of B. cinerea hyphae inside the necrotic lesion, but not in the water-soaked area. Leaves of StDND1 well-silenced potato plants exhibited much smaller blue spots, surrounded by a yellow/brownish ring, indicating a restricted extent of fungal growth surrounded by plant tissue undergoing chlorosis and cell death (yellow/brownish colour). No fungal growth was observed on leaves mock-inoculated with only PDB (Fig. 5). DAB staining showed that the brown colour in the wild type (cv Desiree) control extended well beyond the borders of the lesion, where the fungus could not be detected by GUS staining, suggesting that an oxidative burst occurred in plant tissue at a significant distance from the front of the expanding fungal hyphae. In contrast, the inside of the lesion lost most of its brown colour, as the plant tissue was entirely colonized by the fungus and no longer capable of mounting an oxidative response to the presence of the fungus. In StDND1 well-silenced potato plants, however, the brown colour seemed to be restricted to the necrotic zone surrounding the fungal hyphae, and the entire brown region was smaller than in the control. Moreover, the central part of the inoculation site in StDND1 well-silenced plants was deep brown and not discoloured like in the control (Fig. 5), suggesting a pronounced oxidative burst in the plant tissue in the vicinity of the invading fungal hyphae.
Expression of B. cinerea endopolygalacturonase gene Bcpg1 and cutinase gene BccutA are altered in StDND1 well-silenced potato
For B. cinerea, successful penetration of the cell wall requires enzymes capable of degrading pectin, including endopolygalacturonases (endo-PGs) and pectin methylesterases (PMEs). In order to assess whether the expression of one of the endo-PG genes was affected in StDND1 well-silenced potato plants during infection, the transcript levels of Bcpg1 were investigated from 0.5 to 48 hpi (Fig. 6a). The expression level of Bcpg1 in leaves of cv Desiree and the DND1 weak-silenced potato DND1A-6(−) increased 5–10 fold at 3 hpi and continued to rise until the last time point at 48 hpi. The transcript levels of Bcpg1 in the two StDND1 well-silenced potato transformants DND1A-5(+) and DND1A-17(+) only started to increase somewhat at 10 hpi, while at 24 hpi they reached values that were comparable to the 6 hpi sample on the control plants. Only at 48 hpi did the Bcpg1 transcript levels in StDND1 well-silenced potato plants approach the level in both cv Desiree and potato transformant DND1A-6(−), but the difference in transcript level was still significant.
To assess whether the expression of the B. cinerea cutinase gene was altered in DND1 well-silenced potato plants, the transcript levels of BccutA were also investigated in cv Desiree and StDND1-silenced potato transformants from 0.5 to 48 hpi (Fig. 6b). Similar to the transcript pattern of Bcpg1, significantly lower transcript levels of BccutA were observed in StDND1 well-silenced potato transformants. These results are in agreement with GUS staining (Fig. 3) indicating that the growth of B. cinerea is reduced in StDND1 well-silenced potato plants.
SlDND1 well-silenced tomato plants show reduced susceptibility to Botrytis cinerea
In order to investigate whether silencing DND1 can reduce B. cinerea infection also in tomato, the second generation (T2) of RNAi::SlDND1 tomato plants obtained after transformation of cv Moneymaker (MM)  were used in a DLA with B. cinerea strain B05.10. T2 plants were screened for the presence or absence of the RNAi construct. Compared with the susceptible control MM, similar sizes of necrotic spots were found on non-silenced tomato T2 plants (not carrying the RNAi construct). In contrast, smaller necrotic spots were observed at the inoculation sites on the SlDND1 well-silenced tomato T2 plants (carrying the RNAi construct) and the partially resistant control genotype, Solanum habrochaites accession LYC4 (Fig. 7a). From 1 to 3 dpi, MM and non-silenced T2 plants showed a similar pattern of lesion development (Fig. 7b). The lesion development on DND1 well-silenced tomato plants was slower than that observed on the partially resistant control LYC4. In order to monitor the conidial adhesion, germination and hyphal outgrowth, we performed a histological study with the B. cinerea reporter strain containing a GUS gene under control of the promoter of BccutA, as discussed above for potato. Compared to the susceptible MM plants, a lower number of conidia, delayed germination and reduced germ tube growth of B. cinerea were observed at 12 hpi on SlDND1 well-silenced tomato plants (Fig. 7c). At 16 hpi, B. cinerea hyphae were branching and forming multiple penetration sites on MM plants. At the same time point, on inoculated leaves of SlDND1 well-silenced tomato plants, the growth of hyphae was severely delayed and they were just in the stage of producing appressoria, visible as swellings at the tips of germ tubes. At 22 hpi, B. cinerea further developed expanding necrotic lesions on MM plants, but not on SlDND1 well-silenced tomato plants.
In this study four candidate S genes, DND1, PMR4, DMR6 and DMR1, of which the silencing was previously shown to provide resistance to P. infestans, were investigated via RNAi in potato for their role in susceptibility to B. cinerea. Of these four genes, only DND1 was tested earlier in relation to B. cinerea, and the dnd1 mutant of Arabidopsis showed increased resistance [30,31,32]. Here, it turned out that silencing the DND1 ortholog in potato provided a high level of resistance to B. cinerea. Furthermore, silencing the tomato DND1 ortholog in cv Moneymaker also resulted in reduced susceptibility of tomato to B. cinerea. Based on the microscopic observations of the infection process of B. cinerea on DND1 well-silenced tomato and potato plants, we discuss here the potential mechanisms by which the silencing of the DND1 gene confers reduced susceptibility to B. cinerea.
The DND1 gene encodes a protein that belongs to the family of cyclic nucleotide-gated ion channel proteins (CNGCs; . CNGCs have a role in conducting Ca2+ into plant cells and are involved in various physiological processes . In the Arabidopsis dnd1 mutant AtCNGC2 is disrupted. This mutant exhibits a broad-spectrum resistance in absence of hypersensitive response (HR) to several biotrophic and necrotrophic pathogens including B. cinerea [41, 42]. HR is one of the most effective ways to impede growth of biotrophic pathogens, however it is considered to facilitate the growth of necrotrophic pathogens like B. cinerea . Thus, the resistance of the dnd1 mutant to B. cinerea may be logically explained by the cell death suppression. However, in spite of a deficient HR, a dnd1ein2 double mutant exhibited susceptibility to B. cinerea , showing that the ethylene pathway also plays an important role in the elevated resistance to B. cinerea.
Our results showed that silencing the DND1 ortholog in both potato and tomato led to reduced susceptibility to B. cinerea. The number of attached B. cinerea spores to the plant surface was significantly lower on DND1 well-silenced potato and tomato plants than on wild type plants. In literature, two steps of conidial attachment were described for B. cinerea. The first is the weak adhesion to the plant surface via conidia and germ tubes (between 0 to 6 hpi) and the second is a stronger adhesion through appressoria (between 6 to 10 hpi). In our study, similar numbers of blue-stained conidia were found on all potato genotypes at 0.5 hpi (Fig. 2), suggesting germination rate is comparable among these genotypes. At 3, 6 and 10 hpi, significantly lower numbers of conidia was observed on DND1 well-silenced potato plants, suggesting the adhesion or attachment was impaired. However, in tomato, conidial germination was delayed on RNAi silenced plants compared to that on the control MM plants. Although this is not the case in potato, we cannot rule out the possibility that the conidial germination might be impaired on DND1 silenced potato plants. Further experiments, such as using B. cinerea strains expressing fluorescent proteins and stains like WAG Alexa 488, are helpful to monitor conidial germination, attachment and hyphal penetration on DND1 silenced tomato and potato plants.
Although it is uncertain, our data may indicate that cuticles of DND1 well-silenced potato and tomato plants are chemically or physically altered. The cuticle consists of a complex polymeric network of esterified hydroxylated fatty acids covered with a wax layer, and it serves, amongst others, as a structural barrier that protects epidermal cells against pathogens . Several cutin-defective Arabidopsis mutants have been identified showing resistance to B. cinerea, including sma4 (also known as bre1 or lacs2), bodyguard (bdg) and lacerate (lcr) (Additional file 1: Table S1). Spore germination and hyphal elongation of B. cinerea were inhibited on the leaves of the sma4 mutant . All these mutants are defective in cuticle integrity and have an increased cuticular permeability. The latter may allow the diffusion of toxic compounds with growth-inhibiting activities, as shown in the bdg and lacs2 mutants [29, 45]. In our study, GUS staining of B. cinerea indicated that at 10 hpi, the hyphae appeared to be dead on the DND1 well-silenced potato plants (Fig. 2). This would be in favour of the hypothesis of diffusion of fungitoxic compounds through a defective cuticle.
There is evidence that cuticular permeability can be uncoupled from resistance to B. cinerea. For example, the Arabidopsis Resurrection 1 (rst1) mutant exhibits normal cuticular permeability although it has an altered cuticle composition . This mutant shows resistance to B. cinerea, which is associated with attenuated SA-dependent responses and enhanced JA-dependent defence. This resistance was compromised by defective JA and ethylene signalling pathways.
In addition, plant cell walls have a surveillance system. Alteration of cell wall integrity can lead to specific activation of novel defence pathways as in the Arabidopsis myb46 mutant . The transcription factor MYB46, a key player in regulating secondary cell wall biosynthesis, functions as a susceptibility gene to B. cinerea by downregulation of CesA genes .
We cannot rule out the possibility that the SA signalling pathway plays a role in the weakened B. cinerea infection on leaves of DND1 well-silenced potato and tomato plants. In StDND1 well-silenced potato plants, constitutively elevated PR1 expression was observed . In Arabidopsis, it has been shown that both JA- and SA-mediated signalling are required for local resistance to B. cinerea . The Arabidopsis dnd1 mutant displays elevated SA levels, which could be required for its resistance to a broad range of pathogens including B. cinerea. It is, therefore, worthwhile to investigate relative expression of SA, ET and JA pathway marker genes in DND1 well-silenced potato and tomato plants in order to understand the impact of DND1 silencing on the pathogen’s infection process.
In tomato, resistance to B. cinerea is observed in the ABA-deficient sitiens mutant by a timely hyper induction of H2O2-dependent defences in the epidermal cell wall . Further, it was suggested that ABA determines susceptibility to B. cinerea by negatively regulating SA signalling . In the tomato sitiens mutant, H2O2 accumulation was observed from 4 hpi in epidermal cell walls in close contact to the fungal germ tubes . In our study, H2O2 accumulation was associated with the lesion growth in the susceptible control cv Desiree, while in DND1 well-silenced potato plants, lesions were surrounded by a ring of chlorotic tissue where H2O2 accumulation was also present (Fig. 4). It is unclear whether H2O2 accumulating in the yellow ring contributes to the resistance mechanism or reflects an early indicator of a pathway to cell death.
In addition to the DND1 gene, silencing the DMR6 ortholog in potato showed a significantly reduced susceptibility only at 3 days after inoculation. While, silencing the DMR1 or PMR4 orthologs in potato did not reduce the susceptibility to B. cinerea. In Arabidopsis, dmr1 mutants showed resistance to obligate (hemi-)biotrophic oomycetes and fungal pathogens, including Hyaloperonospora arabidopsidis, Oidium neolycopercisi, Fusarium culmorum and F. graminearum [50,51,52]. The DMR1 gene encodes a homoserine kinase and the resistance in the Arabidopsis dmr1 mutants was associated with accumulation of homoserine. In pea, it has been reported that homoserine and asparagine trigger the expression of pelD (pectin-inducible pel gene D) to assist the pathogenic process of the necrotrophic root pathogen Nectria hematococca . It is unclear whether accumulating amino acids plays a role in resistance/susceptibility to B. cinerea. In the dmr1 mutants, the SA-dependent defence marker gene PR-1 was not induced . In contrast, the Arabidopsis pmr4 mutants show constitutive activation of SA-dependent defences . The PMR4 gene encodes a callose synthase required for callose deposition in papillae and pmr4 mutants are resistant to powdery mildews [52; 55]. There is evidence showing that SA accumulation rather than lack of pathogen-induced PMR4 callose synthase activity is the cause of resistance in pmr4 mutants to powdery mildews. The DMR6 gene encodes a 2-oxoglutarate Fe (II) dependent oxygenase. It was shown that resistance to Pseudomonas syringae and Phytophthora capsici in Arabidopsis dmr6 mutants was associated with elevated SA levels . Many studies have demonstrated that SA suppresses JA signalling . Since the SA signalling pathway is essential for resistance toward biotrophic organisms, while JA mounts defence against necrotrophic organisms , it is an intriguing question whether SA pathway play a role in reduced susceptibility only to P. infestans by silencing PMR4 and DMR6 in potato.
Our results showed that silencing the DND1 ortholog in both potato and tomato led to reduced susceptibility to B. cinerea (this study) as well as powdery mildew and P. infestans . Although further studies need to be performed to examine whether structural changes of epidermal cells and/or hormone signalling are involved in the reduced susceptibility of DND1 well-silenced potato and tomato plants, our results demonstrate that knocking-down plant susceptibility genes may open a new way for breeding crops with resistance to all kind of pathogens including B. cinerea. The reported Arabidopsis genes (Additional file 1: Table S1) that facilitate B. cinerea infection offer a convenient start for a targeted homology-based approach in finding and modifying their orthologs in crops, as performed in this study. Most of these Arabidopsis genes are involved in the cell wall biogenesis and altered plant morphology is detected in their mutants (Additional file 1: Table S1), which can hamper their application in crops due to fitness costs. The Arabidopsis dnd1 mutant showed dwarfing and auto-necrosis, and also the DND1-silenced potato and tomato plants were obviously affected in their development and fitness under the growth conditions used. However, fitness costs are not always associated with mutants in cell wall architecture. For example, the Arabidopsis rwa2 mutant displayed both a normal plant morphology and resistance to B. cinerea . Moreover, new techniques, such as CRISPR/Cas9 [59, 60] are available to create allelic loss-of-function variants, which combine favourable disease resistance levels with less detrimental side effects. With this technology it is even possible to create tetra-allelic gene disruption in tetraploid potato in one step .
In this study, we tested whether impairing the functionality of four S genes, DND1, DMR6, DMR1 and PMR4, in potato/tomato would confer resistance to B. cinerea. Our results showed that silencing DND1 significantly reduced susceptibility of potato and tomato to B. cinerea. Microscopic studies showed that conidial germination and attachment as well as hyphal growth of B. cinerea on the leaf surface of DND1-silenced potato and tomato plants were hampered. Further studies need to be performed to elucidate the precise mechanisms for resistance to B. cinerea conferred by silencing DND1 in plants. Our findings demonstrate that knocking-down plant susceptibility genes can open a new way for breeding crops with resistance to B. cinerea.
pHellsgate plasmids with the S gene silencing fragments of StDMR1, StDMR6, StPMR4 and StDND1  were transferred to A. tumefaciens strain AGL1 + virG through electroporation. RNAi transformants of potato cv Desiree for those four S genes were previously described . Three-week old rooted in vitro plants were transplanted into soil and grown in the greenhouse at 75% relative humidity under 16-h light/ 8-h dark conditions. After 4–6 weeks, when the plants were at 9-10th leaf stage, the fourth or fifth fully developed leaf (counted from the bottom) was selected for inoculation. One composite leaf with five leaflets was kept in floral foam in a plastic box covered with a transparent lid.
For StDMR1, StDMR6 and StPMR4, two well-silenced transformants for each gene were used in a DLA. For StDND1, three StDND1 well-silenced transformants (DND1A-5, DND1A-8 and DND1A-17) and one weakly-silenced transformant (DND1A-6) as well as wild type cv Desiree were used for DLA. Transformants DND1A-6, DND1A-5, DND1A-17 and cv Desiree were used for staining of fungal hyphae and analysis of defense responses. Four cuttings from each genotype were used in the analysis. Tomato SlDND1-silenced T2 plants were obtained by selfing primary transformants as described previously . Tomato cultivar Moneymaker (MM) and wild relative Solanum habrochaites accession LYC4, showing reduced susceptibility to B. cinerea , were grown with SlDND1-silenced T2 plants in the greenhouse. The 4-6th leaves were harvested from 4 to 5 week-old tomato plants and used for analysis.
Inoculation and sample collection
The leaflets were inoculated on the adaxial side with 6–8 droplets of 2 μl of the wild type B. cinera strain B05.10 or a GMO reporter strain of B. cinerea (strain SAS56 with a pCutGUS;  spore suspension in PDB (Potato dextrose broth, 12 g/L) at a density of 3 × 105 spores/ml. At the same time, extra plants from each genotype were mock-inoculated with only PDB medium as a control. After inoculation, leaves were incubated in closed plastic trays to obtain a humidity of 100% at 20 °C (16 h light / 8 h dark). In the DLA, the lesion diameter on leaflets was measured using a calliper with digital display (DIGI-MET®, Helios Preisser, Germany) at 1, 2 and 3 days post inoculation (dpi). For GUS (beta-glucuronidase) and DAB (3,3′-diaminobenzidine) staining, leaf discs (including the inoculation sites) were punched out with a 1-cm-diameter cork borer at six time points (0.5, 3, 6, 10, 24, and 48 hpi), from five leaves (three inoculated biological replicates plus two mock-inoculated controls) per time point. Leaf samples were collected for RNA extraction at each time point.
For histochemical localization of GUS activity, leaf discs of potato and tomato were incubated with 0.5 mg/ml X-Gluc (5-bromo-4-chloro-3-indolyl b-D glucuronide, Biosynth AG) in 50 mM phosphate buffer (pH 7.0, 1 mM KFeCN and 0.05% (v/v) Triton-X100) overnight at 37 °C. The staining solution was removed and leaf discs were washed with 96% ethanol until tissue was cleared. The tissue was mounted on microscope slides in 50% (v/v) glycerol.
In order to detect H2O2 generation in detached plant leaves, DAB staining was performed. Leaf discs were placed in a solution of 1 mg/mL 3,3′-diaminobenzidine dissolved in 0.2 M PBS (phosphate buffer) and HCl was used to adjust the pH to 3.8. The leaf samples were placed overnight in light to optimize the staining reaction. The samples were cleared by boiling with 96% ethanol until the leaves were decolorized and transferred to fresh 70% ethanol for storage until microscopic examination.
Twelve preparations were observed for each genotype per time point with a bright field microscope (Zeiss, Germany). Pictures were taken with a Canon PowerShot A620 camera mounted on the microscope. The presence of H2O2 is visualized as a brown coloration.
Gene expression analysis
Leaf samples were collected at 6 time points (0.5, 3, 6, 10, 24, and 48 hpi) and stored at −80 °C. Leaf material, when taken from the −80 °C freezer, was kept in liquid nitrogen until grinding by using mortar and pestle. Total RNA was extracted by using the MagMAX-96 total RNA Isolation kit (Ambion). RNA was treated with RNAse-free DNAse (Qiagen). The concentration of isolated RNA was measured by using the Isogen Nanodrop Spectrophotometer ND-1000. cDNA was made by using the iScript cDNA synthesis kit (Bio-Rad). For the determination of relative transcript levels, the iQ SYBR Green supermix (Bio-Rad) and the C1000™ Thermal Cycler PCR system (Bio-Rad) was used. Housekeeping genes EF1α (plant) and actin BcactA (Botrytis) were used for normalization. Relative transcript levels were determined using the 2-ΔΔCt method . Gene-specific primer pairs were designed with Primer 3.0 (http://bioinfo.ut.ee/primer3-0.4.0/primer3/, Additional file 5: Table S2). Three technical replicates were used.
cyclic nucleotide-gated ion channel
Clustered regularly interspaced short palindromic repeats/ CRISPR-associated protein-9 nuclease
detached leaf assay
defence no death
days post inoculation
hour post inoculation
long-chain acyl-CoA synthetase
Potato dextrose broth
quantitative trait loci
- S :
the second generation
5-bromo-4-chloro-3-indolyl b-D glucuronide
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We thank Dirk Jan Huigen and Gerda van Engelenhoven for maintenance of the potato plant material. We thank Danny Schipper, Mathijs Peters, and Anindita Chakraborty for assisting in the microscopic studies.
This research was made possible by a financial grant from TKI Uitgangsmaterialen (project number EZ-2012-07) and a grant from University Fund Wageningen.
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Possible Botrytis cinerea S genes identified in Arabidopsis (according to Van Schie and Takken ). (PDF 84 kb)
Detached leaf assay (DLA) of potato RNAi transformants with Botrytis cinerea (strain B05.10). a-d Lesion diameter on the inoculated leaves of the StDMR1, StDMR6, StPMR4, or StDND1 well-silenced potato RNAi transformants. Two to four independent transformants per gene were used. For StDND1, four independent transformants were used, one weakly-silenced transformant (−), and three well-silenced transformants (+). Susceptible control was cv Desiree. One leaf (the 5th or 6th leaf) with 5 leaflets per plant was drop inoculated (6 to 8 drops per leaf). Data were collected at three time points: 1, 2, and 3 days post inoculation (dpi). An average of all lesion diameters per transformant and per time point was calculated. (TIFF 933 kb)
Detached leaf assay (DLA) of potato RNAi transformants with Botrytis cinerea (strain B05.10). Infection symptoms on cv Desiree, StDMR1, StDMR6, and StPMR4 well-silenced transformants. One leaf (the 5th or 6th leaf) with 5 leaflets per plant was drop inoculated (2 to 4 drops per leaf). Photos were taken at 3 days post inoculation (dpi). (TIFF 1419 kb)
Differences in interaction of B. cinerea with cv Desiree or with StDND1-silenced potato plants at different time points after inoculation (Scale bar = 100 μm). The photos were taken at 0.5 h post inoculation (hpi), 3 hpi, 6 hpi, 10 hpi and 24 hpi, respectively. (TIFF 4981 kb)
Primers used in this study. (PDF 143 kb)
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Sun, K., van Tuinen, A., van Kan, J.A.L. et al. Silencing of DND1 in potato and tomato impedes conidial germination, attachment and hyphal growth of Botrytis cinerea . BMC Plant Biol 17, 235 (2017). https://doi.org/10.1186/s12870-017-1184-2
- Botrytis cinerea
- Infection progress
- Plant disease
- Susceptibility gene