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Fig. 7 | BMC Plant Biology

Fig. 7

From: Silencing of DND1 in potato and tomato impedes conidial germination, attachment and hyphal growth of Botrytis cinerea

Fig. 7

Infection progress of Botrytis cinerea on SlDND1-silenced tomato transformants at different time points after inoculation. a Disease symptoms of B. cinerea on leaves of cv Moneymaker (MM), S. habrochaites genotype LYC4 and SlDND1-silenced tomato plants. b Lesion development in T2 progeny (T2#2 family derived from T1#2, Sun et al. 2016) harbouring a SlDND1 silencing construct (+, n = 8) or their non-transgenic siblings (−, n = 2). Data were collected at 1, 2, and 3 days post inoculation (dpi). For each genotype, five leaflets per plant were inoculated with B. cinerea (strain B05.10). c Infection progress of B. cinerea (strain SAS56 containing pCutGUS) on MM and SlDND1 well-silenced tomato plants at four time points after inoculation (Scale bar = 100 μm). The small black arrows mark hyphae extending below the plane of focus (at 16 hpi in MM and 22 hpi in RNAi DND1 silenced plants). The photos were taken at 0.5, 12, 16, and 22 h post inoculation (hpi), respectively. The photos are representative for the results observed in six preparations per genotype

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