Fig. 6

Relative transcript levels of the Botrytis polygalacturonase gene Bcpg1 and the cutinase gene BccutA in cv Desiree and StDND1-silenced potato transformants during infection with B. cinerea (strain SAS56 with a pCutGUS). Infected leaves were sampled at 0.5, 3, 6, 10, 24, and 48 h post-inoculation (hpi) for RNA extraction. For each sample, the transcript level of Botrytis gene Actin was used as reference, the relative expression level of the target gene in cv Desiree leaves at time point 0.5 hpi was defined as 1. Data represent the mean of three biological replicates with error bars displaying the standard error. Three technical replicates of each repeat were analysed, which showed similar results. Asterisks indicate degree of significance compared to cv Desiree plants (*p < 0.05, **p < 0.01, ***p < 0.001)