ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance
- Oumaya Bouchabke-Coussa†1, 6,
- Marie-Luce Quashie†2, 6,
- Jose Seoane-Redondo†3, 6,
- Marie-Noelle Fortabat6,
- Carine Gery6,
- Agnes Yu4, 6,
- Daphné Linderme5, 6,
- Jacques Trouverie6,
- Fabienne Granier6,
- Evelyne Téoulé6 and
- Mylène Durand-Tardif6Email author
© Bouchabke-Coussa et al; licensee BioMed Central Ltd. 2008
Received: 08 September 2008
Accepted: 07 December 2008
Published: 07 December 2008
Drought is a major social and economic problem resulting in huge yield reduction in the field. Today's challenge is to develop plants with reduced water requirements and stable yields in fluctuating environmental conditions. Arabidopsis thaliana is an excellent model for identifying potential targets for plant breeding. Drought tolerance in the field was successfully conferred to crops by transferring genes from this model species. While involved in a plant genomics programme, which aims to identify new genes responsible for plant response to abiotic stress, we identified ESKIMO1 as a key gene involved in plant water economy as well as cold acclimation and salt tolerance.
All esk1 mutants were more tolerant to freezing, after acclimation, than their wild type counterpart. esk1 mutants also showed increased tolerance to mild water deficit for all traits measured. The mutant's improved tolerance to reduced water supply may be explained by its lower transpiration rate and better water use efficiency (WUE), which was assessed by carbon isotope discrimination and gas exchange measurements. esk1 alleles were also shown to be more tolerant to salt stress.
Transcriptomic analysis of one mutant line and its wild-type background was carried out. Under control watering conditions a number of genes were differentially expressed between the mutant and the wild type whereas under mild drought stress this list of genes was reduced. Among the genes that were differentially expressed between the wild type and mutant, two functional categories related to the response to stress or biotic and abiotic stimulus were over-represented. Under salt stress conditions, all gene functional categories were represented equally in both the mutant and wild type. Based on this transcriptome analysis we hypothesise that in control conditions the esk1 mutant behaves as if it was exposed to drought stress.
Overall our findings suggest that the ESKIMO1 gene plays a major role in plant response to water shortage and in whole plant water economy. Further experiments are being undertaken to elucidate the function of the ESKIMO1 protein and the way it modulates plant water uptake.
Understanding plant response to abiotic stress is of interest to both basic and applied research. Recently, our knowledge of the mechanisms developed by plants to sense and transfer stress signals, and then orchestrate gene expression in order to protect and/or repair tissues and cells, made rapid progress . Nevertheless, many questions regarding these mechanisms, which are of great importance in biology, remain to be answered. At the same time, maintaining agricultural supply in a fluctuating environment is a major challenge for the XXIst century. Crop yield losses induced by environmental stress are estimated to reach 60–70% [2, 3]. A major challenge over the coming decades is to develop plant varieties with reduced requirements for water and other inputs and which also maintain stable yields in diverse environmental conditions.
The overall response by plants to environmental constraints has been well characterised and extensively reviewed [1, 4–8]. Stress from the environment leads to both specific and common effects and responses. Drought is particularly complex because it leads to simultaneous physiological responses at the whole plant, cellular and molecular levels. For example, drought induces mechanical stress on roots due to soil hardness , osmotic stress because of cell dehydration and removal of water to the extra-cellular space , and oxidative stress by the accumulation of reactive oxygen species (ROS) . During cold and salt stress the physiological response is similar to that caused by drought [12, 13], meaning that the effects of different environmental stresses are tightly interconnected.
Stress sensing is still an unknown process: the nature of the first physical or chemical signal remains hypothetical . Signal transduction is better understood, but remains complex because of the crosstalk between different signalling pathways . It involves diverse molecular mechanisms such as protein phosphorylation , modifications to membrane phospholipids which affect membrane fluidity and release signal molecules such as inositol triphosphate (IP3) and changes Ca2+concentration in the cytosol ... Drought and salt stress trigger ABA production, which in turn induces the expression of a number of responsive genes. Many but not all stress response genes respond to ABA [18, 19]. ROS can also be important signalling molecules [11, 20, 21], and stimulate Ca2+, ABA and MAPK cascades.
Genes induced by stress can be roughly classified into two groups: genes coding for regulatory proteins, mainly transcription factors, and genes encoding proteins involved directly in response mechanisms; genes from both classes are of interest. Variations in the expression of regulators could lead to a protective status before the emergence of stress and have multiple effects. Genes involved in protection or repair mechanisms could be new targets for the improvement of plant plasticity and adaptive responses to stress . The unraveling of general stress responses in the model species Arabidopsis thaliana helped to identify potential targets for plant breeding. Arabidopsis genes involved in tolerance to abiotic stress were transferred, by genetic engineering, to many crops and tolerance was successfully conferred in the field, despite the complexity of plant responses to environmental stress [23–28]. Thus, finding new key genes responsible for abiotic stress tolerance phenotypes is of great importance not only for a better understanding of stress responses, but also for promising future crop improvement.
Our team is involved in a plant genomics programme where a series of candidate genes was analysed for their role in environmental stress responses, using Arabidopsis thaliana insertional mutants . A list of candidate genes and corresponding mutants was compiled by an in silico search for Arabidopsis genes with homology to maize and/or wheat genes which showed modified expression in response to water deficit, salt or cold stress http://urgi.versailles.inra.fr/GnpSeq. A mutant line in the ESKIMO1 gene was retained both in the cold and drought screens because it responds to stress differently to wild type. Initially Xin and Browse  identified the eskimo1 mutation as conferring freezing tolerance without cold acclimation. They observed that a significantly high proline content accumulates in esk1 mutants as a mechanism to balance the osmotic stress. Ghars et al.  observed a similar proline content in wild type and esk1 mutant, but proline accumulation was higher in esk1 in response to salt stress. Xin et al.  identified the eskimo1 mutation by positional cloning. The gene product belongs to an uncharacterised plant-specific protein family containing 48 members. Bioinformatics analysis of genes whose expression was modified by the eskimo1 mutation showed that a large number were previously reported to be induced by salt, osmotic stress and the stress hormone ABA, however Xin et al. did not consider that the mutant is drought or salt tolerant.
In this article, we describe the response by ESK1 allelic mutants to different abiotic stresses in two genetic backgrounds (WS and Col-0). We found that the mutant lines have a clear advantage in response to drought and salt stress, but at the cost of biomass production. Nevertheless, this cost could be compensated by the maintenance of growth over a large range of environmental conditions. Based on physiological tests and transcriptomic analysis we could formulate a hypothesis regarding ESKIMO1 function. Our results are discussed in relation to those reported by Xin and coworkers .
Characterisation of the esk1-6mutant line
• Without abiotic constraint
General characterization of the esk1 mutant lines
• Response to monitored mild water-deficit
Monitored stress: experimental system on propagation plugs
At time 0, propagation plug saturation was 100%
(SMWC = Substrate maximum water content)
Propagation plug saturation
Mild water deficit
Severe water deficit
All the plants were watered daily to reach 60% SMWC with a volume based on the average weight of a subset of propagation plugs
All the plants were watered daily to reach 30% SMWC with a volume based on the average weight of a subset of propagation plugs
Not done (threshold effect)
Each plant was watered daily to reach 60% SMWC based on the actual weight of the propagation plug
Each plant was watered daily to reach 30% SMWC based on the actual weight of the propagation plug
Each plant was watered daily to reach 20% SMWC based on the actual weight of the propagation plug
Salt stress: individual determination
Each plant was watered daily to reach 60% SMWC based on the actual weight of the propagation plug. Once at 60%, salt stress was applied with 0.5× a nutritive solution supplemented with 150 mM NaCl.
Not done (combined water deficit and salt stress not applied)
Not done (combined water deficit and salt stress not applied)
Total Leaf Area analysis of the segregating esk1-6 T3 population
• Response to cold
Viability of the wild-type genetic background and respective mutants after freezing.
% viability mutant
(wild type) % viability wt
• Response to osmotic stress
We observed that the treatment and genotype had a significant effect on the TLA and PRL (p < 0,001) but a significant genotype × treatment interaction was only seen for the TLA (p < 0,001).
Analysis of esk1alleles in the Col-0 genetic background
• Without abiotic constraint
• Response to cold
Homozygous esk1-4 and esk1-5 lines were subjected to the freezing test described in the Methods section and the viability was scored. Both mutants exhibited higher tolerance than Col-0, when exposed to freezing after acclimation (Table 4). However without previous acclimation, all the plants of both the mutant lines and wild type died.
• Response to mild water deficit
TLA was calculated for the homozygous mutant lines and wild type after 7 days of averaged mild water deficit and control conditions (Table 2). The TLAs of the esk1-4 and esk1-5 mutant lines were reduced by 42% and 46% respectively compared to the TLA in standard conditions whereas the wild type TLA was reduced by 16% (Figure 2B).
• Response to salt
We observed germination and plantlet growth in vitro on control medium and medium supplemented with 100 mM, 150 mM and 200 mM NaCl. The NaCl concentration had no effect on germination but did have a significant effect on root and leaf growth (treatment effect), but the mutant lines and Col-0 responded in the same way and there was no genotype × treatment interaction (data not shown).
• Response to osmotic stress
Osmotic stress was applied in vitro. Col-0, esk1-4 and esk1-5 were grown on standard medium or medium supplemented with 75 mM mannitol. TLA and PRL were assessed on plantlets (Figure 4B and 4D). We observed a treatment effect in all the analysis. There was no significant genotype effect on PRL, in control or osmotic stress conditions. There was a genotype effect on TLA for esk1-5 only, in control and osmotic stress conditions.
• Water starvation
• Water consumption and Water Use Efficiency (WUE)
Carbon isotopes discrimination for esk1-4, esk1-5 and wild type
• Transcriptome analysis of the esk1-5mutant line and Col-0
The Col-0 and esk1-5 transcriptome was analysed by individually determined control, severe drought and salt stress conditions (Table 2). The experimental conditions were the same as those used for WUE assessment (Figure 11). Two biological replicates were used. Each replicate was a pool of three plants. For this study, data were normalized and the p-value was adjusted using the Bonferroni method, with a 0.05 threshold. Among the Gene Sequence Tags (GST) probes present on the CATMA array, only those corresponding to nuclear genes annotated at TIGR were used in this study (21 788 uniques genes). Among these, only genes which had the same expression profile between the two biological replicates were considered.
We set up a screen to identify a list of the genes that were either not expressed at all or weakly expressed (around the background) in wild type but over-expressed or highly repressed in the esk1-5 mutant, in the three conditions (Additional file 1). The experimental background was set at around 7.5 and an intensity of less than 9 corresponded to low expression. In the following section, we only refer to genes that can be discussed in an eskimo1 context.
Among the genes that are strongly over-expressed in esk1-5 in control conditions and weakly expressed in wild type, we selected: GSTF12, a member of glutathione S-transferase gene family among which each gene shows a particular inducibility by stress ; CAX3 (Calcium Exchanger-3) involved in ion homeostasis ; DFR (Dihydroflavonol Reductase) which is involved in the flavonoid biosynthetic pathway and also responds to environmental conditions ; ATHB-7 (Homeobox Leucine Zipper-7) a transcription factor induced by water deficit and by ABA ; PR2 a Pathogenesis-Related gene involved in the acquisition of systemic resistance : all these genes are potentially involved in general defence responses. Other genes identified are noteworthy for their implication in development, such as MBP2 (Mirosinase-binding protein-2 ), or metabolism, such as MAM-3. (Methylthioalkylmalate-3 ). RDR-2, a RNA-dependant-RNA-polymerase-2, is involved in chromatin modifying via small-interfering RNA pathway . NIA-1, the Nitrate reductase-1 and NCED-4, a nine-cis-epoxy-carotenoid-dioxygenase (or CCD4, Carotenoide Cleavage Dioxygenase) obtained lower scores (respectively r = 1.92 and 1.60) but are also worth mentioning.
Among the genes that are under-expressed in the esk1-5 mutant in control conditions, GLP-3, a germin-like protein obtained a very high score (r = 6.29). Scores were lower but still significant for potentially interesting metabolism genes: KCS-8, a 3-ketoacyl-CoA synthase; a FLS or Flavonol synthase and CSD-2, a superoxide dismutase. Two genes might be involved in signal transduction: FLA2, a fasciclin-like arabinogalactan which shows a rapid decrease in response to ABA  and PRP4 which is a structural Proline-rich protein.
It is striking that under drought conditions only 11 genes were seen to be over-expressed or repressed in the esk1-5 mutant and none of these are expressed more than 5 times. Nevertheless, NIA1 appears to be over-expressed. A gene encoding XTR3, which belongs to a Xyloglucan endotransglucosylase/hydrolase family , is repressed in the mutant but there is no evidence that this particular member plays a role in the cell wall construction and we did not observed any difference between the cell wall composition of wild type vs. esk1-5 and esk1-4, based on Fourier-Transform Infrared microspectroscopy profiles  (data not shown). APT3, SAD1 and/or KAT5 (one GST hybridises with SAD1 and KAT5) are also repressed in esk1-5. A mutation in SAD1 (Super Sensitive to ABA and Drought) led to hyper-reactivity to drought stress and ABA . KAT5 encodes a putative 3-ketoacyl-CoA thiolase. APT3 encodes an Adenine phosphoribosyltransferase and may contribute to cytokinin metabolism .
The situation is more complex under salt stress: 61 genes were over-expressed and 107 genes are under-expressed in the esk1-5 mutant. NIA1 is strongly over-expressed in the three conditions. We also noticed some genes that are known to be induced by low temperature, dehydration and ABA: LTI30 (previously called XERO2) belongs to the dehydrin or LEA (Late Embryogenesis Abundant) family [46, 47], RD29B or Responsive to Dehydration29B is also known to be induce by salt [48, 49]; COR78 or Cold Regulated78 or RD29A . DREB2A (Dehydration Responsive Element-Binding protein2A) which is not induced by ABA is also over-expressed in the mutant . Among the genes repressed in esk1-5 compared to wild type under salt stress, some were also repressed under control conditions: GLP3 obtained a very high ratio (r = -7.06); AT2G10940 and AT2G15090 are annotated as being involved in the storage and metabolism of lipids, respectively; AT1G04800 is annotated as being involved in N-terminal protein myristoylation, a mechanism that could play a role in regulating signals produced by salt stress . Several other interesting genes are repressed in the mutant: βCA1, a carbonic anhydrase-1 (in plants, Carbonic anhydrases are involved in the fixation of inorganic carbon); UBC6 contains an Ubiquitin conjugating (UBC) domain and Plasma membrane intrinsic protein1;5 (PIP1;5) and Tonoplast intrinsic protein2;2 (TIP2;2) are both aquaporins. Aquaporins are involved in water uptake from the soil and root hydraulic conductivity . ABA2 encodes a xanthoxin dehydrogenase involved in the synthesis of ABA .
The Eskimo1 mutation was first identified as a mutation conferring frost survival without an acclimation period . We did not observe this type of freeze tolerance in our experimental system, i.e. with plantlets grown in soil, either with the original esk1-1 mutant line, or three independent insertional mutant lines. Xin and Browse, however, carried out frost tests in vitro and we did them in soil, which might explain the reason for the phenotypic differences observed. We applied abiotic stress to plants in soil rather than in vitro because it is closer to field conditions. Our experimental system and results are more similar to those of Reyes-Diaz et al. , who worked with plants in pots, at the 10–15 leaf development stage and did not observe any difference in freezing tolerance without acclimation between the esk1-1 mutant line and its wild type genetic back-ground. They reported that both the wild type and the esk1-1 mutant can tolerate freezing only after a cold acclimation period and that without acclimation, the two genotypes avoid freezing by delaying or preventing frost damage. Here, we also clearly showed that ESKIMO1 mutants are more tolerant to freezing but only after acclimation (Table 4).
Drought and salt responses
In a recent article, Xin and collaborators found that the esk1-1 mutation was not involved in drought and salt stress responses . Originally, we selected esk1-6 as a candidate gene after an in silico analysis because it has sequence similarities with a maize EST that changes expression in response to cold treatment. We screened for drought and cold response independently and selected the ESKIMO1 mutant in both screens. We observed significant differences in the response to mild drought, water starvation and cold stress between soil grown wild type and esk1-6 at the 6th leaf stage (Figures 2, 3; table 1, 2, 3). No differences in root growth were observed in vitro following salt and osmotic stress (Figure 4). The two independent mutant lines esk1-4 and esk1-5 showed similar phenotypes (Figure 2, 3, 4, 5, 6, 7, 8, 9, Table 1, except for the PRL in vitro, Figure 4) and responded to stress the same way. Therefore, the phenotype differences can be confidently assigned to the ESKIMO1 mutation. The phenotype of the esk1-6 mutant which has an insertion in the promoter region is slightly different (Figure 2, Figure 3). Progeny tests showed that the esk1-6 mutation is recessive (Table 2). Thus the slight differences observed between esk1-6 and esk1-4 and esk1-5 are most likely due to the different genetic background and/or changes in ESKIMO1 expression. In summary, the general characteristics observed for the three mutant lines were highly similar and can be clearly attributed to the mutation in ESKIMO1.
We showed that in standard and drought conditions, the mutants' transpiration rate was lower than that of the wild type. We suspect that stomatal conductance is lower in the mutant which is supported by the result showing slower "Cut Rosette Water Loss". However, we also determined that the transpiration results cannot be explained by reduced stomatal density, which was actually higher in the mutant.
Water Use Efficiency
Since the esk1 mutants are smaller than wild type plants, their water needs are expected to be lower, but the parameter which is of biological relevance is water required per biomass unit. WUE was assessed by measuring CO2 consumption and H2O release with a portable gas exchange system. Our results clearly show that the WUE of the esk1-5 mutant is higher than the wild type. Due to the small size of the mutant leaves, it was not possible to assess to the gas exchange under stress conditions with the previous system. In addition, this type of measurement is taken at selective time point so that the results can vary depending on the metabolic state of the leaf at the measurement time. Thus we choose to use an alternative method based on carbon isotope discrimination (δ13C). Carbon fixation during photosynthesis discriminates against the heavy carbon isotope (13C) . Because WUE is highly correlated to carbon isotope discrimination, δ13C can be measured as a reliable indicator of WUE. This correlation has been observed in wheat  and in Arabidopsis thaliana . The results showed that the two allelic mutants have a higher WUE (Table 5) than the wild type. We also observed that the WUE of both wild type and mutant plants improved slightly following drought treatment but that salt treatment does not seem to affect WUE. Because δ13C reflects the isotope discrimination signature for the life-time of the plant, it is not surprising that a three day stress did not affect this measure. It is more surprising, however, that we observed a general tendency for an improvement in the WUE, in the three genotypes, under drought conditions, after only 4 days of reduced soil water content. All together, these results show that the esk1 mutant has an improved WUE and a higher photosynthetic rate. In a review article, Parry et al.  postulated that this is achieved in three possible ways: a CO2 concentrating mechanism, increased mesophyll conductance or increased performance of rubisco (D-ribulose 1,5-bisphosphate carboxylase/oxygenase).
Transcriptomic analysis showed that under control conditions 985 genes are differentially expressed between wild type and the esk1-5 mutant (Figure 12) but only 57 of these genes are still differentially expressed in drought conditions. It can be clearly seen in figures 12, 13, 14 and 15 that the transcriptomes of the wild type and mutant are similar under mild water deficit stress, but not in control conditions. We hypothesise that the mutation in the ESKIMO1 gene leads to a physiological response preparing the plant for drought stress, explaining why some genes involved in stress responses were already expressed during the watering regime. In line with this theory, a large proportion of genes which are differentially expressed between the wild type and mutant were assigned to functional categories related to defence and environmental interactions. We propose that the other functional categories differentiating the wild type from the mutant are a consequence of a perturbed metabolism in the mutant. The proportion of differentially expressed genes is larger under salt stress than under control condition. Even if there is a lot of crosstalk between abiotic stresses like drought, cold, osmotic and salt stress, the ESKIMO1 gene appears to specifically mimic water depletion. Both drought and salt stress sensed by the plant will progressively lead, depending on their intensity, to osmotic stress caused by cellular dehydration . Drought also has a mechanical stress component due to soil hardening , and salt stress has an ionic component which may be toxic and induce specific genes. The fact that the "structural molecule activity" category (ribosomal proteins) is repressed in the mutant may also mimic abiotic stress: down-regulation of genes involved in protein synthesis was described in Populus euphratica in response to salt stress  and in maize in response to osmotic stress . Also of note, the "protein biosynthesis" category is down-regulated and "carbon utilisation" is up-regulated in citrus in response to gibberellins . Genes related to abiotic stress, mainly water response, were differentially expressed in this study.
Two genes annotated as transcription factors were reported to be highly over-expressed in the esk1-1 and esk1-4 mutants in Xin et al.'s article and were also identified in the esk1-5 mutant in our control conditions. Plants in Xin's experiment were grown in vitro and harvested at 14 days. In our conditions plants were grown on propagation plugs and harvested a week after bolting. As a consequence, we can postulate that these two genes, AT1G18710 and AT2G46680, are major contributors to the expression of the phenotype in the eskimo1 background.
We found that one of the two nitrate reductase genes, NIA1, is highly over-expressed in the mutant in the three conditions. The other NR gene, NIA2 is expressed in both esk1-5 and the wild type, but its expression is slightly higher in the mutant under control and drought stress conditions. This may reflect improved carbon assimilation in the esk1 context, regardless of the environmental conditions, because this process has been correlated with NR activity . Elsewhere, NR was found to be required for stomatal closure in an ABA-dependant pathway, by generating the signalling molecule nitric oxide . This mechanism could maintain the stomata closed in the esk1-5 mutants depending on nitric oxide signalling.
We also observed that a number of genes that play or that may play a role in general defence responses are over-expressed in esk1-5 in control conditions. These genes are listed in the supplementary material and are described in results section. Nevertheless, none of the known key players in stress response such as the transcription factors DREB2A, DREB2B and CBF4, responsive genes RD29A and RD29B, or genes involved in salt response from the Salt Overly Sensitive family... were found to be differentially expressed. Thus the low evapo-transpiration stress symptom of the esk1-5 mutant under control conditions may reflect a different mechanism than that typically induced by the bulk of stress responsive genes. We observed that three aquaporins are repressed in the mutant: a Tonoplast Integral Protein (AT3G16240 or DELTA-TIP), and two Plasma membrane Intrinsic Proteins (AT4G23400 or PIP1;5, AT3G54820 or PIP2;5). One PIP is over-expressed in the mutant in control conditions (AT3G61430 or PIP1A). Thus, an overall hydraulic disruption in the mutant genotype might be a signal for stomatal closure. One aquaporin (AQN1) in Nicotiana tabacum is located in the chloroplast membranes and facilitates CO2 diffusion and assimilation . However, more experiments are needed to pinpoint the precise role of the aquaporins differently expressed between wild type and esk1-5 mutant. Several genes that are usually associated with drought stress are differentially expressed between wild type and the esk1-5 only under salt stress: RD29A and RD29B, DREB2A and LTI30. These four genes also gave much higher hybridisation signals on the CATMA microarray under drought stress than under control conditions (the background noise was around 7.5 and the four genes showed signals between 10.07 and 13.40 under drought stress). This suggests that their expression is affected by drought stress but they are highly induced by salt only in the esk1-5 mutant background.
Our results can be discussed in light of those of Xin and coworkers, who carried out water starvation tests on wild type and mutant plants (esk1-1) growing in the same pots, and concluded that the mutation was not associated with an increased ability to survive drought or salt stress. We observed that wild type consumes more water than the mutant lines (esk1-4 and esk1-5), so it is not surprising that in the same pot, the wild type would first use up the available water, exhausting the substrate for all the plants. Once a critical soil water potential is reached, eskimo1 mutants are not different from wild type. We propose that the eskimo1 mutants take more time to exhaust the water from a given substrate and convert it to biomass more efficiently. Another significant difference between our findings and those of Xin and collaborators is that they did not observe a difference in the effect of salt on wild type and mutant plants (esk1-1). Again, the experimental conditions were very different in the two studies. Their salt response experiment was carried out in vitro with seedlings three days after germination, and indeed, we also failed to observe any difference in the response to salt by young plants in vitro. We hypothesise that the results we obtained with mature plants in response to salt, i. e. the wild-type but not the mutant leaves presented lesions close to the meristem, is a consequence of differences in the plant water economy. It is likely that, in wild type the water-salt solution was pumped from the soil faster than in the mutant and caused damage to the plants.
It also seems that the phenotype we observed in our large mutant screen was not, in the strict sense, a response to drought: the eskimo1 mutant uses less water which means that the substrate will dry more slowly. Therefore, rather than being tolerant to drought per se, the mutant can overcome a water deficit period more easily than wild type. Nevertheless, our phenotype screen is accurate because we selected the eskimo1 mutant due to its severely disturbed response to drought stress and it would not have been selected by observing in vitro responses to osmotic or salt stress (our results).
Based on our findings, we conclude that the ESKIMO1 gene plays a major role in whole plant water economy. We determined that the eskimo1 mutation leads to a loss in fitness, but in drought conditions most of the wild type died whereas the mutant lines keep producing seeds. We are currently generating transgenic lines in which the ESKIMO1 gene will be inactivated in response to abiotic stress in order to minimise this fitness cost of the mutation but maximise survival and WUE under drought stress. We are also searching for natural alleles of ESKIMO1 that could change the expression of the gene and/or the functionality of the protein. Condon et al. reported the release of new varieties from breeding selection for δ13C to improve WUE and grain yield in wheat . ESKIMO1 has homologous genes in numerous species. It is tempting to speculate that allele selection or manipulation of ESKIMO1 in crops could improve WUE.
Plant response to abiotic stress is a complex trait divided among distinct but cross talking pathways. Expression of the regulators of the genes involved in the response is itself tightly regulated [5, 67]. We are particularly interested to know if the ESKIMO1 gene is a negative regulator of stress response as postulated by Xin et al.  or if the induction of abiotic stress genes in the mutant line(s) is a secondary consequence of the plant water status due to a water uptake deficiency.
Mutant lines in the AT3G55990 gene were obtained either from the INRA Resource Centre for Arabidopsis thaliana Genomics http://dbsgap.versailles.inra.fr/portail/: esk1-6 in the WS genetic background , or from The Salk Institute in the Col-0 genetic background: SALK_078275 (esk1-4) and SALK_089531 (esk1-5) . The WS and Col-0 lines used were from the INRA Versailles Resources Centre: 530AV and 186AV.
Drought, cold and salt treatments
• Monitored stress applied in propagation plugs
Arabidopsis plants were grown following standard procedures established by Loudet et al. . Seeds were stratified for 4 days in a 0.1% (w/v) agar solution at 4°C in the dark. Germination occurred 2 days after sowing on propagation plugs (4 cm height × 4 cm radius – 70% blond peat, 20% perlite and 10% vermiculite, Fertiss®). Plants were grown under long day conditions with a 16 h photoperiod, in a controlled environment chamber (22°C, 70% RH, PPFD approximately 150 μmol m2 s-1) and watered with nutritive solution as described in Bouchabke et al. . The relationship between soil volumetric water content and soil suction was previously assessed .
During plant growth prior to starting the stress experiments the propagation plugs were saturated with nutritive solution (100% at t0). During the stress experiments, however, propagation plugs were weighed daily from t0. Once the target saturation was reached, this was then maintained for the duration of the experiment. For controls, soil water content was fixed at 60% of substrate maximal water content (SMWC). The mild water-deficit treatment was fixed at 30% SMWC whereas severe water-deficit corresponded to 20% SMWC. Two approaches were used to control the saturation level, either an averaged or an individually monitored stress, as indicated in Table 2. For the averaged determination method, the weight of ~10% of the propagation plugs was measured and all the plugs adjusted with the average volume calculated to reach the targeted saturation. For the individual determination method each propagation plug, within a set watering regime, was maintained at the same saturation level based on its actual weight. In this way, the substrate saturation of all the genotypes within a watering regime was identical regardless of their water consumption. The individual determination, which is more labour intensive was employed for the experiments where result reproducibility was most likely to be affected by slight differences in the stress imposed, namely the transcriptome and carbon isotope discrimination analyses (Table 2, Figure 11). To measure integrative parameters such as TLA or CRWL, stress was monitored using the averaged determination of the propagation plugs. Experimental start points varied depending on the phenotype examined; for TLA and CRWL t0 was when leaf number 6 emerged (growth stage 1.6 according to Boyes et al. ), For transcriptome and carbon isotope discrimination, treatments were applied from floral bud emergence onwards (growth stage 5.10 according to Boyes et al. ).
Salt stress experiments were conducted in parallel to those for drought stress. The propagation plugs for treated plants were first reduced to 60% saturation before the salt stress was applied by watering with 0.5× nutritive solution supplemented with 150 mM NaCl. In this way, plants were subjected to each stress for the same time period.
• Water starvation
Plants were cultured in peat moss in pots (length 60 mm, width 65 mm, height 60 mm), filled equally with a homogeneous non-enriched compost (Terf® Substrat: 37% blond peat, 60% brown peat, 10% volcanic sand). The pH of this compost was stabilised between 5.5 and 6.1.
Plants were grown in the same environmental conditions as described above. Progressive drought was applied on 9 randomly selected one-month-old plants of each line (stage 5.10 according to Boyes et al. ) by stopping watering. As a control, the same number of plants of each line was grown under standard irrigation conditions and watered twice a week. Pictures of the canopy were taken at day 0, 6 and 10 of stress exposure to calculated the Photosynthetic Leaf Area. PLA is equal to TLA minus chlorotic areas.
• Cold treatments
Seeds were stratified as described earlier (Monitored stress applied in propagation plugs). Then a large-scale screen to test cold tolerance was performed as follows: rows of plants were sown in square pots containing organic substrate and irrigated with mineral nutrient solution once a week and watered every four days. Plants were grown in the greenhouse for 14 days at which time they had reached the 6–8 leaf stage (stage 1.04 according to Boyes et al. ). Plants were then transferred to a growth chamber at 5°C under 12 h photoperiod, 70 μM m-1s-1 light intensity and 70% relative humidity for 7 days. Acclimated plants were then exposed to freezing temperatures of -8°C for 48 h. After this cold treatment, plants were put back in the greenhouse. Tolerance to freezing was determined by evaluating the percentage of viability after freezing exposure: viable and dead plants were counted and the percentage viability was calculated. Four rows of the mutant and two rows of the reference strain were put in each square pot to optimise viability comparisons by reducing undesirable environmental variation. At least 400 plants were tested per line. Parallel experiments were carried out without the acclimation period.
• Salt treatments
Plants were grown in pots as described for the water starvation experiment. For 12 days, 3 × 10 plants of each line were watered every two days with a concentrated saline solution (NaCl 200 mM). Every three watering cycles plants were watered with non-saline water. Results were compared with the same number of plants grown with standard irrigation. Pictures were taken at day 0, 6 and 10 of stress exposure.
For culture on agar plates, seeds were sterilised, stratified four days at 4°C in the dark and then transferred onto 3 × 10 plates of solid medium . Plants were cultivated in growth chambers under long-day conditions (16 h/d) at a photon flux density of 120 μmol m-2 s-1. Temperature (21°C) and relative humidity (70%) were constant in the growth chamber. For in vitro salt stress induction, the solid medium was supplemented with 3 different NaCl concentrations (100; 150; 200 mM). The same number of standard media plates was prepared as a control. For leaf development studies, 9 × 3 seeds from each line were randomized at regular intervals inside the plate. For root development analysis, six seeds were placed (from each genotype) on each plate, close to one of the edges. Plates were laid horizontally for 48 hours and then placed vertically in a rack, with the seeds at the top. All plates were collected at day 12 and scanned with a desktop scanner (Epson scan Photo 4990) using the "transparent object" mode at 300 dpi.
• Osmotic treatments
Osmotic stress was induced following the same protocol as for salt stress in solid media, but supplemented with 60 or 75 mM mannitol.
Fresh weight, dry weight, water loss and transpiration
Fresh weight (FW) was obtained by harvesting and weighing freshly cut rosettes (stage 3.70 to 3.90 according to Boyes et al. ).
Rosette dry weight was recorded after 48 h at 75°C in a dry oven.
Relative Water Content (RWC) was calculated according to the formula: [(FW-DW)/DW] × 100.
Cut Rosette Water Loss (CRWL) indicating the amount of water lost from freshly cut tissues during the first 60 minutes, was determined by harvesting and weighing freshly cut rosettes. Rosettes were maintained in the growth chamber conditions then weighed every 10 minutes. CRWL was then calculated as the ratio between water loss and plant initial fresh weight, expressed in %.
To assess transpiration in planta the rosettes of 6 plants per genotype and per soil water treatment were isolated from the soil with a plastic film. The entire propagation plug was also covered with plastic film preventing any soil evaporation. Propagation plugs without rosettes were also included in the experiment to assess water evaporation from empty propagation plugs. Two hours after the beginning of the light period, plants were weighed every two hours for 36 hours. Transpiration per unit of dry weight was then calculated as the ratio between transpiration (weight of the propagation plug at tx time, minus weight of the propagation plug at t0, minus evaporation from empty propagation plugs) and the plant dry weight.
The number of stomata per leaf area was determined on the 10th or 11th leaf of five plants grown in control conditions in short days, in the greenhouse (stage 1.13 to 1.14 according to Boyes et al. ). Leaves were fixed in ethanol/acetic acid (3/1) for one hour, and then washed three times with pure water. After this step, they were bleached in NaOH 8 M for one hour, then washed three times with pure water. The surplus water was wiped away. The leaves were mounted in a 0.1% calcofluor solution and observed with a F.I.S.H (Fluorescent In Situ Hybridization) microscope at a 350 nm wavelengh (UV light), magnification 12.5×. Six pictures of each leaf were taken on the whole leaf surface excluding the central nervure. The stomata were counted with ImageJ software using the "cell counter" plugin.
Gas exchange measurements
Gas exchange was measured from one leaf of three independent plants of each genotype (Col-0 and esk1-5) using a portable gas exchange system (Li-6400; LI-COR) with a standard leaf chamber (6400-40 with red and blue LED light source; LI-COR). Leaf chamber conditions were 400 μmol mol-1CO2, 21% O2, 51.47% relative humidity, 22°C and photosynthetic photon flux density (PPFD) of 500 μmol m-2 s-1 with 10% of blue light. Leaves were kept under this condition for approximately 30 min until parameters were stabilized before recording. Because Col-0 and especially the esk1-5 leaf were too small to fill the entire area of the leaf chamber, the portion enclosed parts of leaves were marked. The leaves were then cut from plants, scanned and the total leaf area (which had been enclosed in the chamber) was evaluated by image analysis using a similar procedure as described in the data processing section below. Net photosynthesis  and transpiration (ET) were calculated by using equations derived by Caemmerer and Farquhar . WUE was estimated by the net photosynthesis/evapo-transpiration ratio (NP/ET).
After 7 days of individual determination of treatments, frozen samples were lyophilized and then ground. For each sample, about 1 mg of powder was transferred into tin cups (Courtage analyse service, Mont Saint-Aignan, France) and analysed in an elemental analyser (NA-1500, Carlo Erba, Milan, Italy) coupled to an Isotope Ratio Mass Spectrometer (VG Optima, Fison, Villeurbanne, France). Carbon isotope compositions were calculated as deviations of the carbon isotope ratio (13C/12C, called R) from international standards (Pee Dee Belemnite) according to Farquhar et al.: δ13C = |103 [(Rsample - Rstandard)/Rstandard]|.
For the transcriptome analysis, RNA was extracted with the RNeasy extraction kit from Qiagen® including the DNase treatment. Microarray analysis was carried out at the Unité de Recherche en Génomique Végétale (Evry, France), using the CATMA array [76, 77], containing 24,576 Gene-Specific Tags from Arabidopsis. RNA samples from two independent biological replicates were used. For each biological replicate, RNA samples for a condition were obtained by pooling RNA from 3 plants. For each comparison, one technical replicate with fluorochrome reversal was performed for each biological replicate (i.e. four hybridisations per comparison). RT on RNA in the presence of Cy3-dUTP or Cy5-dUTP (Perkin-Elmer-NEN Life Science Products), hybridisation of labelled samples to the slides, and the scanning of the slides were performed as described in Lurin et al. . Microarray data from this article were deposited at Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/; accession No. GSE10384) and at CATdb (http://urgv.evry.inra.fr/CATdb/; Project RA06-02_StayGreen) according to the "Minimum Information About a Microarray Experiment" standards.
Rosette surface measurements were performed as followed: rosette surfaces were selected using Photoshop® software (selection/colour range) and saved as .tif files. Files were then opened with ImageJ software and transformed to 8-bit. The global scale was set with the help of a ruler in the initial picture, the threshold was adjusted to optimise the selection and the area was measured. Statistical analyses were performed with Statgraphics® software. We used the term Total Leaf Area (TLA) for a green rosette and Photosynthetic Leaf Area for a damaged rosette. PLA corresponds to the area of non-damaged leaves.
For the transcriptome analysis, experiments were designed with the statistics group of the Unité de Recherche en Génomique Végétale. Statistical analysis was based on two dye swaps (i.e. four arrays, each containing 24,576 GSTs and 384 controls) . Controls were used for assessing the quality of the hybridisation, but were not included in the statistical tests or the graphic representation of the results. For each array, the raw data comprised the logarithm of median feature pixel intensity at wavelengths 635 (red) and 532 nm (green). No background was subtracted. In the following description, log ratio refers to the differential expression between two conditions. It is either log2 (red/green) or log2 (green/red) depending on the experimental design. Array-by-array normalisation was performed to remove systematic biases. First, we excluded spots that were considered badly formed features. Then, we performed global intensity-dependent normalisation using the LOESS (locally weighted scatterplot smoothing) procedure to correct the dye bias. Finally, for each block, the log ratio median calculated over the values for the entire block was subtracted from each individual log ratio value to correct print tip effects on each metablock. To identify differentially expressed GSTs, we performed a paired t-test on the log ratios, assuming that the variance of the log ratios was the same for all genes. Spots displaying extreme variance (too small or too large) were excluded. The raw p-values were adjusted by the Bonferroni method, which controls the FWER (Family-Wise Error Rate). We considered genes as being differentially expressed with a FWER of 5%. We used the Bonferroni method (with a type I error equal to 5%) in order to keep strong control of false positives in a multiple-comparison context . A manual clustering step was carried out only considering GSTs with the same expression pattern in the two biological replicates. In this manuscript, any differentially expressed GST that hybridises with two genes (genes with an identification number in The Arabidopsis Information Resource or TAIR) is accounted as two distinct genes. A Perl script was developed to select genes which were highly expressed or highly repressed between wild type and mutant, and expressed at around the background level in wild type or mutant (with a high log2 (ratio) range and with a log2 (red or green intensity values) of around 7.5). Results are presented in the Additional file 1. Analysis of the functional categories of genes according to the Gene Ontology and the whole Arabidopsis thaliana genome annotation was made with the TAIR GO annotation tool http://www.arabidopsis.org/tools/bulk/go/index.jsp September 3rd, 2008.
We are especially grateful to our initial Biogemma and INRA partners who performed the in silico screening to establish the list of candidate genes: P. Perez, J. Rouster, N. Sajot, P. Lessard, S. Aubourg and A. Lecharny. We thank C. Lelarge, J. Ghashghaie and G. Tcherkez for carbon isotope discrimination measurements using the equipment of the "metabolism-metabolome" platform IFR87, Orsay and to G. Mouille from the "Plant Chemistry Platform", INRA Versailles, who analysed the cell wall composition. We thank M. Simon and S. Durand who participated in the initial mutant screen. We are grateful to J.-P. Renou (transcriptome), L. Chelysheva (stomatal density), C. Camilleri and O. Loudet (every topic) for scientific discussions and V. Lefebvre, Y. Chupeau and G. Pelletier for their critical reading of the manuscript. MQ was supported by a post-doctoral fellowship from the AUF (Agence Universitaire de la Francophonie). JSR received a fellowship "VERT" from the Marie Curie Host Fellowships for Early Stage Research Training (EST), 6th Framework Programme for Research & Development of the European Union. JT was supported by the ANR07GPLA003 programme (Agence Nationale pour la Recherche/Génoplante).
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