Comparative analysis of the transcriptome during single-seed formation of Castanea henryi: regulation of starch metabolism and endogenous hormones
BMC Plant Biology volume 23, Article number: 90 (2023)
In seed plants, the ovule is the precursor to the seed. The process of ovule development and differentiation is regulated by multiple factors, including starch metabolism and endogenous hormones. Castanea henryi produces nuts with high nutritional value. However, the high proportion of empty buds restricts the commercial use of the tree. Previous studies have shown that the empty bud phenotype is closely related to ovule abortion. If none of the ovules in the ovary expand rapidly and develop in 7–8 weeks after pollination, an empty bud will form. Therefore, we studied the development and molecular mechanisms underlying single seed formation in C. henryi.
We found that 49 days after pollination (DAP) is a critical period for the formation of fertile and abortive ovules. The morphology and starch distribution of the fertile and abortive ovules differed significantly at 49 DAP. The fertile ovules were smooth and round in appearance, with a large amount of starch. In contrast, abortive ovules were smaller with only a small amount of starch. The embryo sac of the abortive ovule proceeded to develop abnormally, and the entire ovule lacked starch. We identified 37 candidate genes involved in metabolism with potential roles in the regulation of starch levels. Three ADP-glucose pyrophosphorylase (AGPase) genes, one granule-bound starch synthase (GBSS) gene, and two beta-amylase genes could affect starch accumulation. The levels of auxin, cytokinins, gibberellins, and jasmonic acid in fertile ovules were higher than those in abortive ovules. In addition, the levels of endogenous abscisic acid and salicylic acid in abortive ovules were higher than those in fertile ovules of the same age, consistent with the expression patterns of genes related to the synthesis of abscisic and salicylic acid and signal transduction. We identified and mapped the differentially expressed genes associated with hormone synthesis and signal transduction.
These results improve our general understanding of the molecular mechanisms underlying single seed development in C. henryi and the phenomenon of empty buds, providing directions for future research.
Ovules carry the female gametophyte, which develops into a seed after fertilization. This is indispensable for the formation of seeds and the normal growth of angiosperms . In addition to being consumed by humans, seeds also have a high economic value, as they are used for the production of animal feed and raw chemical materials, and the research and development of medicine . Crop yield often depends on the quantity and quality of the seeds, while the quality of each seed depends largely on the number, size, and proper development of the ovules . Although each ovule has the potential to be fertilized until it forms viable embryos, obstacles arising during sexual reproduction can prevent some ovules from developing. The occurrence of ovule abortion may be affected by physiological, biochemical, embryological, environmental, and molecular factors, including male sterility , female sterility , poor pollination or fertilization , temperature , and water . Consequently, understanding the mechanisms of ovule development is of great importance from both scientific and economic perspectives. The ovule development process is regulated by several factors, including starch metabolism and endogenous hormone regulation. Starch actively participates in the metabolic processes of embryonic development and regulates the dynamic balance of carbohydrate metabolism in plants. Moreover, starch is the most significant means of carbohydrate storage and therefore the primary energy store in some seeds, particularly in Castanea.
Starch metabolism is an essential part of seed development, and has a direct impact on grain yield and quality. Starch metabolism in plants is aided by a variety of complex and coordinated mechanisms governed by several enzymes [9, 10]. The key processes and pathways involved in starch metabolism in higher plants include starch synthesis and degradation. In storage tissues, sucrose is hydrolyzed into monomeric sugars by invertase (InVS) and sucrose synthase (SUSY) to form uridine diphosphate glucose (UDP-glucose) and fructose . Subsequently, these products are transformed into glucose-1-phosphate (G-1-P) by UDP-glucose pyrophosphorylase. G-1-P forms adenosine diphosphate glucose (ADPG) through the action of ADP-glucose pyrophosphorylase (AGPase). ADPG is a direct precursor of starch synthesis and synthesizes amylose under the action of ADP-Glc pyrophosphorylase (AGPase) and granule-bound starch synthase (GBSS) [11, 12]. ADPG synergizes with AGPase, soluble starch synthase (SS), SBE (starch branching enzyme), and the starch debranching enzyme (DBE) to synthesize amylopectin. Synthesized starch is mainly degraded by alpha-amylases (AMY), beta-amylases, or through the phosphorylation pathway . AMY hydrolyzes the alpha-(l,4) glycosidic bonds of amylose and amylopectin in the substrate molecule . Beta-amylase cleaves α-(1,4) glycosidic bonds from the non-reducing ends of polysaccharides to generate maltose .
Endogenous hormone regulation also plays an important role in embryonic development . Among them, auxins play an important role in the formation of female gametophytes and sporophyte organization. l-Tryptophan aminotransferase of Arabidopsis1(TAA1) encodes a key protein involved in auxin synthesis, which is under-expressed in developmentally deficient ovules . Cytokinins (CKs) negatively regulate cell proliferation in the sporophyte tissue surrounding the developing female gametophyte. Therefore, a lack of CKs leads to an increase in the number of cells in the ovule, which in turn leads to an increase in seed size . Gibberellins (GAs) regulate ovule development. In a previous study, treating flowers with an exogenous GA spray significantly increased the proportion of sweet cherries with nacreous ovules, prolonged the embryo sac life span, and increased the seed setting rate . Abscisic acid (ABA) plays an important role in embryonic development, promoting the synthesis of storage proteins during seed maturity; consequently, a sharp increase in ABA is an important factor leading to embryo abortion . Jasmonic acid (JA) regulates ovule senescence, and the JA signal response factor PbMYC2 can directly activate the transcription of senescence-related genes, thereby inducing the death of pear ovules. Salicylic acid (SA) also contributes to ovule abortion . These studies demonstrate the synergistic effect of multiple hormones in regulating embryonic abortion in plant species.
Castanea henryi is a tall deciduous tree of the Fagaceae family. The nuts of C. henryi have a smooth and attractive appearance, and their sweet taste and nutritional value are making them increasingly popular among consumers [22, 23]. The production of C. henryi has become a pillar industry allowing farmers to increase their income in certain mountainous areas of China. However, the high rate of empty chestnut buds leads to low production and reduced income . Considering the increasing demand for chestnuts in China, studying the key factors affecting the formation of empty buds and finding a way to artificially increase chestnut yield are urgent problems to be solved. Previous studies have shown that the rate of empty bud production is closely related to ovule abortion [25, 26]. Currently, research focusing on the development of C. henryi ovules is limited, and there have been no studies on macromolecule dynamics during single seed formation. In this study, we studied the external morphology of ovule development, starch distribution, metabolic enzyme activity, and endogenous hormone levels during the development of fertile and abortive ovules. Candidate genes related with starch biosynthesis metabolism and hormone synthesis were identified by sequencing the transcriptomes of selected developmental stages.
Microscopic observation on the formation of fertile and abortive ovules
Castanea henryi has a compound pistil with a multi-carpel ovary and congruent style. The ovaries are wrapped with closed or semi-closed thorn buds. The ovules are all born at the ventral suture of the ovarian wall and are surrounded by many placental hairs. There are many ovules in one ovary. However, no matter what way of pollination (self pollination, outcrossing and natural pollination), only one seed will be produced (Fig. 1). The transverse diameter of the C. henryi ovary was always larger than the longitudinal diameter, and the growth rate of the transverse diameter was also higher than that of the longitudinal diameter at 35–70 days after pollination (DAP). The ovary diameter had a growth peak at 42–49 DAP, and the growth rate suddenly slowed at 49–56 DAP (Fig. 2I). In light of the ovary anatomy, there were usually 16–24 ovules in each ovary. Except for one fertile ovule that could develop normally, the rest of the ovules were aborted (Fig. 2II). At the outset, the size of each ovule in the same ovary was relatively consistent with no significant difference. At 49 DAP, it can be seen that one ovule being slightly larger than other ovules is the fertile ovule. The mean transverse and longitudinal diameters of the aborted ovules reached their maximum values at 49 DAP (Fig. 2I). Subsequently, the fertile ovules gradually become rounded in shape and milky white in color, full, and plump. In contrast, the abortive ovules begin to shrink and shrivel at the micropylar end, have a yellowish brown and gradually extend downward; indicating that 49 DAP describes a critical period for the formation of fertile and abortive ovules (Fig. 2II).
Observation of starch distribution in ovule during single seed formation
To investigate the changes in starch metabolism during single-seed formation, we observed the distribution of starch. Although fertile and infertile ovules cannot be distinguished by assessing the external morphology at 35–49 DAP, they can be classified based on the paraffin section. The starch distribution in fertile ovules washigher than that in abortive ovules at 35–70 DAP. The starch distribution in fertile ovules showed an increasing trend, with a sudden decrease at 49–56 DAP and a rapid increase at 56–70 DAP (Table 1). Initially, starch grains were mainly distributed in the inner and outer integuments and chalazal ends of the fertile ovules. With the continuous development of the ovule, starch grains were greatly reduced, with only sporadic starch distribution on both sides of the fertile ovule integument (Fig. 3I). At 63–70 DAP, starch distribution greatly increased. The starch grains were almost entirely distributed over the fertile ovule, with a tight distribution and large starch grains (Fig. 3II).
In aborted ovules, the starch distribution was greatest at 42 DAP and then gradually decreased until no starch distribution was observed (Table 1). First, starch grains were mainly distributed in the outer integument and chalazal end of the aborted ovules, while a small amount of starch was distributed in the inner integument. This was followed by the gradual decrease of starch distribution in the aborted ovules (Fig. 3I). Although, the abortive ovule develops normally, the embryo sac fails to do so and shows a long and narrow gap structure, and the entire ovule is completely free of starch grains (Fig. 3II).
Based on these results, three representative developmental stages (35 DAP, 49 DAP, 63 DAP) were selected for the comparative analysis of transcriptome data to better understand the starch metabolism molecules and adjustive mechanisms of ovules during the single-seed setting of C. henryi.
Changes in the contents of endogenous hormones during the formation of single seeds
Several key endogenous hormones, including indoleacetic acid (IAA), trans-Zeatin (tZ), cis-Zeatin (cZ), isopentenyladenine (iP), isopentenyladenosine (iPR), GA (1,3,4,7), abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), were measured during seed formation. The contents of CK and IAA in fertile ovules were higher than those in abortive ovules and those in O (ovules at 35 DAP) (Fig. 4). The levels of ABA, SA, and JA in aborted ovules were higher than those in fertile ovules at the same period (Fig. 4). GA1 and GA4 are biologically active GAs that play major roles in plants , and were more abundant in fertile ovules than in abortive ovules. Moreover, the content of GA4 was substantially higher than that of GA1 (Fig. 4B).
RNA-seq of the C. henryi ovule transcriptome
Fifteen cDNA libraries were generated and sequenced spanning the selected three stages (35 DAP, 49 DAP, 63 DAP) of ovule formation, three repetitions for each stage. After eliminating the adaptor sequences, ambiguous reads, and low-quality reads, with a total of 967.86 GB reads were mapped to the C. henryi genome. And the GC contents ranged from 44. 22–45. 94% and the Q30 values were all greater than 90%. Supplementary Fig S2 depicts the length distribution of the C. henryi reads. Following that, all clean reads were subjected to a de novo assembly, resulting in 254, 102 transcripts. This indicates that the amount and quality of transcriptome sequencing data from C. henryi ovules are relatively high, and that these data can be used to provide a raw data basis for subsequent data assembly.
Unigene assembly, annotation and analysis
Unigene sequences were annotated using BLAST software in the following five databases: NR (NCBI non-redundant protein sequences), GO (gene ontology), KEGG (Kyoto Encyclopedia of Genes and Genomes), Swiss-Prot, and egg NOG (evolutionary genealogy of genes) for comparison. Annotations were made for 59,521 genes. A total of 29,815 genes had been mapped to specific GO term, which were further classified into 50 terms falling under three categories: biological process (23 terms), cellular component (16 terms), and molecular function (11 terms) (Supplementary Fig S3). To discover active biological pathways involved in ovule formation, the collected genes were linked to the KEGG protein database. These genes were classified into 19 terms (Supplementary Fig S4). The top three most enriched KEGG pathways were 'transcription', 'carbohydrate metabolism', and 'folding, sorting, and degradation'.
Analysis of the expression levels and differentially expressed genes
Differentially expressed genes (DEGs) were analyzed to identify starch metabolism candidate genes. The DEG-seq program was used to investigate the expression levels of genes involved in C. henryi ovule development. After filtering, a transcriptome analysis was performed on 4162, 4728, 4907, 4590, 2073, 1153, 5560, and 5724 genes, and comparisons were made between the ovule (O) VS the fertile ovule (BO1), O VS the abortive ovule (SO1), O VS the fertile ovule (BO2), O VS the aborted ovule (SO2), BO1 VS BO2, SO1 VS BO1, SO1 VS SO2, and SO2 VS BO2. The SO1 VS SO2 comparison produced the fewest DEGs (696 genes up-regulated and 456 genes down-regulated), while the SO2 VS BO2 comparison produced the most DEGs (2785 genes up-regulated and 2939 genes down-regulated) (Fig. 5I). A total of 1058 DEGs were common in all ovule developmental comparisons. Only 170 DEGs were identical during the development of fertile and abortive ovules (Fig. 5II). The significant DEGs are depicted by red dots on volcano plots, which were created to identify the transcripts that were significantly changed throughout ovule development (Fig. 5III).
Validation of the selected DEGs by qRT-PCR
Quantitative real-time PCR (qRT-PCR) was used to validate some of the DEGs and ensure that the RNA-seq data was reliable. Nine genes were chosen at random and subjected to qRT-PCR analysis. The fact that the qRT-PCR data were mostly consistent with the high-throughput results, implies that the high-throughput sequencing data were trustworthy (Fig. 5IV).
Identification of genes critical for starch metabolism in C. henryi ovules
Many key genes involved in the metabolism of starch and sucrose have been identified. AGPase, GBSS, SBE, and nucleotide pyrophosphatase (NPPS) are some of the enzymes involved in plant starch metabolism. During ovule development, genes encoding key enzymes took part in starch metabolism displayed distinct expression patterns. According to the known starch and sucrose metabolism pathways [12, 28], ten classes of 37 functional genes were identified in our transcriptome database (Supplementary Table S3).
In the initial step of starch synthesis, AGPase catalyzes the conversion of glucose-1-phosphate into ADP-glucose by consuming ATP and participates in the synthesis of amylose and amylopectin [29, 30]. Through bioinformatic analyses, we identified nine AGPase homologs in the annotated C. henryi transcriptome gene dataset. Among these AGPases homologs, we found that three genes were highly expressed in fertile ovules (BO1 and BO2), with much higher levels than those in aborted ovules (AGPS1, AGP31, and AGP20). The FPKM values were > 900 for AGP31 in BO2 and > 100 for AGPS1 and AGP20 in fertile ovules. The expression levels of MGPX2 and IDD2 in abortive ovules were higher than those in fertile ovules at the same time (Fig. 6).
GBSS is a key enzyme in amylose synthesis. It can add glucose residues in the adenosine diphosphate glucose to the non-reducing end of glucan through the α-1, 4-D-glycosidic bond, and extend the linear chain of glucan . In our results, a GBSS gene (WAXY) was differentially expressed that the expression levels of BO2 were significantly higher than those of SO2. Nucleotide phosphodiesterases (NPPS) can catalyze the hydrolytic breakdown of pyrophosphate and phosphodiester bindings of many nucleotides and nucleotide sugars . In our dataset, three NPPS genes were shown, one of which was tremendously expressed in SO1 and SO2 (PAP27-1). The expression of PAP27-2 was the highest in O. In addition, the expression of the starch branching enzyme (SBE) gene (SBEI) in BO2 was higher than that in other ovules. SBE can cleave the α-1,4-glycosidic bond of the α-1,4-glucan linear donor and simultaneously catalyze the α-1,6-glycosidic bond between the cleaved short chain and the acceptor chain form, resulting in branches .
In this research, five AMY genes, six beta-amylase genes and two genes encoding MAL were identified in our predicted database. Two out of the five genes encoding AMY were highly expressed in SO1 (AMYX3 and RASI1). Except for AMYX3, whose expression levels in SO1 and SO2 were higher than 100, the remaining AMY genes were expressed at low levels. The expression levels of these two MAL genes in the O samples were higher than 100, which was significantly higher than that in the other samples (Fig. 6). Almost all beta- amylase genes showed higher expression levels in abortive ovules than in fertile ovules. The expression levels of β- amylase-related genes in aborted ovules were higher than those in fertile ovules, and the expression levels of BAM9 and BAM4 in SO1 were significantly higher than those in other ovules. The BAM9 expression in SO2 was 18 times higher than that in fertile ovules (BO2) at the same time. These results indicated that beta-amylase might be a vital starch-degrading enzyme during the formation of fertile and abortive ovules.
Sucrose synthase (SuSy) mainly functions to decompose sucrose into UDP-glucose and fructose, providing precursors and substrates for polysaccharides, cell walls, and starch synthesis. In addition, SuSy can catalyze the reversible conversion of UDP-glucose and fructose to sucrose [27, 34]. Sucrose is hydrolyzed into glucose and fructose by invertase (INV). Sucrose phosphate synthase (SPS) converts UDP-glucose and fructose-6-phosphate to sucrose-6-phosphate . We identified seven SuSy genes in the transcriptome gene dataset. The expression level of SUS1 was similar in fertile ovules and abortive ovules at all time, and showed a gradual downward trend. Only one of these genes was highly expressed during the BO2 stage (SuS2-2). The INV gene (INH1) was also highly expressed in abortive ovules, especially in SO1. In this study, we also annotated two SPS genes. However, all SPS genes expression levels were very low in all ovule samples (Fig. 6).
Changes in the activities of key starch enzymes during the formation of single seeds
Considering the results of the differential gene analysis, we selected four representative key enzymes (AGPase, SUSY, α-amylase, and beta-amylase) with high activities for content determination. At 35–70 DAP, the changes of AGPase, SUSY, and AMY were roughly the same and all showed an overall upward trend. Enzyme activities in fertile ovules were higher than those in abortive ovules (Fig. 7A-C). The change in beta-amylase activity was different from that of the above three enzymes. Beta-amylase activity in aborted ovules showed an overall upward trend and increased rapidly after 56 DAP. In contrast, the rate of beta-amylase activity in fertile ovules at 49–56 DAP abruptly decelerated with little change. The beta-amylase activities of aborted and fertile ovules were very similar at 56 DAP (Fig. 7D). We found that the changes in beta-amylase activity were consistent with the changes in starch distribution in the fertile ovules. This further suggests that beta-amylase plays an important role in starch metabolism during the development of the ovule of C. henryi.
Identification of genes critical for hormone biosynthesis and signal transduction in C. henryi ovules
Ovule development is regulated by a complex network of hormones. To better understand hormonal regulation during single seed formation in C. henryi, we focused on the DEGs associated with IAA, CK, GA, ABA, JA, and SA synthesis and signaling. We identified 81 DEGs involved in hormone biosynthesis and signal transduction (Supplementary Table 5). Expression trends for a number of DEGs in red border were consistent trends with corresponding trends in hormone contents in ovules (Fig. 8). Most of these differentially expressed genes were associated with IAA and ABA. Four auxin response factor (ARF) genes (ARF8, 6, 6.1, 18), one IAA gene (IAA14), and six SUAR genes (SUAR36, 36.1, 23, 24, 32, 50) were found to be upregulated in abortive ovules (SO1 and SO2). Among these, ARF6 was highly expressed in abortive ovules (SO1 and SO2), with three times higher expression than that of fertile ovules at the same time. Two IAA genes (IAA11, 29), PIN(3,1b), ARF18.1, and YUCCA5 were upregulated during the formation of fertile ovules (BO1 and BO2) (Fig. 8A).
The main physiological functions of CKs are the promotion of cell division and regulation of cell differentiation, delaying aging, and the degradation of proteins and chlorophyll. CKX (5, 1), AHK (1, 3, 1.1), and ARR17 were upregulated in SO1 and SO2. CKX5 was highly transcribed in SO1 and SO2 (FPKM value of > 1300). Similarly, ARR22, CKX6, and LOG3 were upregulated during the formation of fertile ovules (BO1 and BO2); however, the expression levels were not high.
With regard to GAs, GA20X2, three GASA (1.1, 1.2, 1.3), GID1B, and GAIP were strongly activated in the GA signaling pathway in SO1. GAI and GA20OX were consistently upregulated in aborted ovules. The expression level of GASA4 in SO2 was as high as 1500. The GASA family had the highest expression in ovules compared to other families (Fig. 8). A total of 47 DEGs associated with the ABA signaling pathway were identified. Four PP2C genes, two BZIP genes and CCD1 were highly expressed in abortive ovules. The expression levels of differential genes related to ABA biosynthesis in aborted ovules were higher than that those in fertile ovules. These results suggest that ABA may plays a negative regulatory role in the formation of single seeds (Fig. 8).
With regard to JA, TIY8 and MYC2.2 were upregulated in fertile and abortive ovules, respectively. All DEGs related to SA biosynthesis were up-regulated in aborted ovules (SO1 and SO2) (Fig. 8F).
Screening of key genes in the hormone biosynthesis pathway
To explain the relationship and interaction between the changes in endogenous hormones and the expression of genes related to hormone synthesis and signal transduction during the formation of single seeds of C. henryi, we analyzed the correlation between hormone concentration and its metabolism-related genes. The results showed that the concentrations of GA20OX1, KAO, KAO2, KAO2.1, GA3OX3, and GA were significantly positively correlated. PP2C24 and BZIP9 were significantly positively correlated with ABA concentration while AHP4 and CYP735A2 were positively correlated with CK. TIR16 was significantly negatively correlated with IAA concentrations (Table 2). MYC2.1, MYC2.2, and JMT were significantly positively correlated with the expression level of JA. SABP2.1 was significantly positively correlated with SA concentration, and its expression level was substantially higher in abortive ovules than in fertile ovules. The above genes may be the key genes of hormone synthesis during the formation of single seed of C. henryi.
The cone chestnut fruit has important economic value and is rich in nutrients. Ovule development plays an important role in fruit production. Previous research has mostly focused on the anatomical study of ovule development and the mechanism of ovule abortion. However, little is known about molecular mechanism during single seed formation. In particular, the underlying mechanisms in the metabolism of starch and hormone regulation during C. henryi ovule development remain unknown, and the expression levels of the genes involved in starch metabolism and hormone regulation have not been published.
Starch metabolism during single-seed formation
As one of the major carbohydrates, starch is actively involved in metabolic processes in embryos. Ovule development is accompanied by the accumulation or disappearance of starch and these changes often result in a new developmental stage [36,37,38]. With regard to the ovule starch metabolism, some plants, such as sweet cherries  and hazelnuts , have less starch accumulation in aborted ovules, possibly attributed to insufficient starch content that leads to ovary abortion. In the present study, the starch distribution in fertile ovules showed a sudden decrease at 49–56 DAP, while there was no starch distribution in the abortion ovules from 56 DAP. This contrasts with the regularity of carbohydrate changes in many plant ovules, such as those in rice, where the amount of starch in the grain increases along with the increasing sugar content from the early stages of growth . It is speculated that this contradiction may be attributed to embryonic differentiation and development, accelerated cell division, starch utilization exceeding accumulation, and starch amount gradually decreasing or even disappearing . It is also possible to convert starch into a form that is more stable for the development of stored ovules. A possible hypothesis supports the notion that in the critical stage of the formation of fertile ovules and abortive ovules, a large amount of storage material needs to be consumed, and starch is first used as the main nutrient for ovule growth and development . Additionally, starch may be connected with the double fertilization and subsequent zygotic development of fertile ovules, which consume many nutrients, including starch .
The expression levels of essential enzymes involved in starch and sucrose metabolism were substantially linked with the changes in starch distribution in the C. henryi ovules. Ovule development is a complicated process that needs the coordination and cooperation of many critical factors, especially enzymes. All enzymes in the starch synthesis pathway play crucial roles. Three AGPase, three NPPS genes, one SBE gene, and one GBSS gene were highly expressed during the starch synthesis. The AGPase family showed the highest expression among the five samples. We believe that AGPase may be a key enzyme in starch synthesis. ADPG pyrophosphate acidulase isa key rate-limiting enzyme in the starch anabolic pathway and plays an influential role in the starch synthesis process of maize and various plants .
Starch can be broken down by various enzymes. In this study, BAM was an important starch-degrading enzyme in the single-seed setting process of C. henryi. Its expression level in abortive ovules is much higher than that of other gene families. In other plants, BAM has been confirmed to play an important role. Genome-wide analysis identified 9 BAM genes in Arabidopsis, 13 in maize, and 10 in rice. AtBAM4 acts as a chloroplast regulator in Arabidopsis, is potentially responsive to maltose concentration, and can fine-tune the rate of starch degradation [46, 47]. In potato, StBAM9 interacts with StBAM1 on the surface of the starch grains for further starch degradation [48, 49]. In tobacco, the PrtBAM1 gene increases the amylase activity and promotes starch degradation .
Previous studies have shown that beta-amylase is the main degrading enzyme responsible for the hydrolysis of stored starch and the degradation of transition starch. Beta-amylase hydrolyzes starch to beta-limiting dextrin and beta-maltose [51, 52]. In the present study, the beta-amylase activity of fertile ovules decreased sharply at 56 DAP. It is speculated that the activity of the 49–56 DAP abortive ovules decreased rapidly due to the rapid decrease in the amount of starch and substrates for beta-amylase hydrolysis . Interestingly, the beta-amylase activity was consistent with the starch distribution in fertile ovules, which gradually increased and decreased with the increasing and decreasing beta-amylase activity, respectively. Therefore, it is considered that critical enzymes, such as beta-amylase, are closely related to starch synthesis in fertile and abortive ovules, which are directly related to starch accumulation. However, the specific role of beta-amylase requires further exploration.
Hormonal regulation during single-seed formation
Endogenous hormones are an important class of plant growth regulators, and the interaction of a variety of endogenous hormones regulates the ovule development of plants. In this study, we mainly elucidate the regulatory mechanism of GAs, CKs, ABA, and auxin in the formation of single seeds of C. henryi. In addition, we further explain the relationship and interaction between single seed formation in C. henryi and the key genes involved in hormone biosynthesis and signal transduction (Fig. 9). Multiple studies have shown that GAs play an important role in ovule formation. In a previous study, grapes were sprayed with GA before flowering, and it was found that one of the ways through which GA halted seed development was by damaging the redox system in flowers or berries, which resulted in oxidative damage of seeds and affected the expression of genes related to embryo development . Overexpression of GASA4 in Arabidopsis has a positive effect on seed size, weight, and yield . In pea, mutations in the GA biosynthesis genes LH1 and LH2 greatly affect embryogenesis and lead to the production of sterile seeds . Overexpression of the GA2OX gene in pea and Arabidopsis, which is responsible for inactivating active of GAs, resulted in seed abortion . In this study, the content of gibberellin in fertile ovules was significantly higher than that in abortive ovules, and the content in fertile embryos of 63 DAP was as high as 467 ng/g. Among them, the content of GA4 is the highest, which may play a leading role in gibberellin that promotes the formation of single seed of C. henryi. The GA20X1 was significantly correlated with the gibberellin content during single seed formation in C. henryi. The GASA4 was highly expressed in aborted ovules.
Auxin is mainly involved in regulating cell elongation, differentiation, and division. Auxin has been shown to be involved in regulating the development of the embryo, endosperm, embryo, and seed coat before and after fertilization. The YUCCA gene family is a key gene in the process of IAA biosynthesis. In Arabidopsis, the auxin biosynthesis enzymes YUCCA10 and TARI are expressed in the endosperm . PIN1 plays an important role in both ovule initiation and pistil development . A strong loss-of-function allele of PIN1 induces flower formation without ovules and empty pistils with malformed styles . Previous studies have shown that SAUR is involved in auxin-regulated cell expansion . The expression level of the YUCCA gene family was not particularly high in the transcriptome of chestnut ovule. However, PIN1 was highly expressed in fertile ovules.
Increased pistil CK content can increase pistil morphology and ovule number. CK affects the expression of PIN1 by promoting the expression of SPL and AG genes, thereby affecting the development of pistil ovules . In addition to CKX5, which is significantly expressed in abortive ovules, other CK genes are expressed in fertile and abortive ovules; however, their expression levels are not high. Interestingly, the content of auxin and cytocatenin reached the highest level at 49 DAP of fertile ovules, which may be in a critical period of growth and development, when cell division and elongation are accelerated.
ABA and GA play antagonistic roles in seed development . Comparing the differential expression of genes at different stages of ovule development between seeded and seedless grapes, it was found that the genes related to ABA synthesis and transduction pathway are upregulated expression in 'seedless' grapes . The content of ABA in aborted ovules was higher than that in fertile ovules, which was consistent with the expression levels of key differentially expressed genes involved in ABA synthesis. Similarly, the content of SA in abortive ovules was substantially higher than that in fertile ovules, and the results were consistent with the expression levels of key differentially expressed genes related to SA biosynthesis. SA may have the same effect as ABA, both of which promote the abortion of ovules and the formation of single seeds.
In this study, external morphological observations indicated that 49 DAP is a critical period for the formation of fertile and abortive ovules. We investigated the molecular mechanism involved in starch metabolism and the regulation of endogenous hormones in the ovules of C. henryi by a transcriptome analysis at three developmental stages. Critical genes related to starch metabolism were further confirmed by enzyme activity assays. We identified ten classes of 37 functional genes related to starch and sucrose metabolic pathways. Among these, beta-amylase may be a crucial starch-degrading enzyme, and the BAM gene may also be an important determinant of the regulation of starch degradation. The contents of auxin, CKs, GAs, and JA in fertile ovules were higher than those in abortive ovules. We also identified and mapped the DEGs associated with hormone synthesis and signal transduction during the formation of single seeds. In summary, we evaluated endogenous hormones and starch metabolism during single seed formation in C. henryi, providing a preliminary theoretical basis for explaining the reasons for single seed formation in C. henryi.
Plant materials and growth conditions
All methods were carried out in accordance with relevant guidelines. The Chinese chinquapin (Castanea henryi) cultivar ‘Huali 4’(XiangS-SC-SH-010–2015) was used in this study, and the pollinated cultivar was ‘Huali 2’(XiangS-SC-SH-008–2015). These plants were planted on the western campus of the Central South University of Forestry and Technology in Changsha, Hunan Province, China. The C. henryi fruits were obtained from ten 10-year-old trees at six stages of development (35, 42, 49, 56, 63, and 70 days after pollination, DAP). Twenty seeds were selected for each stage to remove the shell and skin for sampling, fixed in FAA fixative [70% ethanol: glacial acetic acid: formalin (18:1:1, v/v/v)] for 24 h, and then stored in 70% alcohol at 4 °C.
Three representative stages (35 DAP, 49 DAP, 63 DAP) were selected for RNA extraction. The seeds were removed from the fruits, and the shell and skin were removed from the collected seeds. The samples were wrapped in foil, frozen in liquid nitrogen, and kept at -80 °C until they were used for RNA extraction analyses.
Dynamic growth of fruit and ovule
The developing fruits were measured in the above six stages using a Vernier caliper (Mitutoyo 530, Japan). Twenty fruits were used to measure transverse and longitudinal diameters at each stage. Subsequently, the mean measures were calculated for each period. Using a stereomicroscope (Olympus SZX16, Japan), we photographed and observed the morphology and size of ovules and ovaries.
Observation of starch distribution
The distribution of starch in the ovules was observed using the paraffin section method combined with periodic acid-Schiff (PAS) staining. Previous research by our group found that the starch grains of C. henryi ovules were stained by PAS and their starch grains were purple-red (Supplementary Fig S1 A-C). An inverted microscope (Leica S/M432299, Germany) was used to observe the green fluorescence (460–550 nm). The imaging position of the starch grains was consistent with the position under non-fluorescence imaging, and its color formation effect was better than that of non-fluorescence imaging (Supplementary Fig S1 a–c). Thus, we used an inverted microscope with green fluorescence to photograph and observe ovule starch.
Measurements of hormone contents
In the present study, ovules with three representative stages (35 DAP, 49 DAP, 63 DAP) were selected for hormone extraction and determination following the methodology established in previous studies . Phytohormones were extracted from approximately 0.5-mg (fresh weight) samples. The GAs, indoleacetic acid (IAA), ABA, c-Zeatin (cZ) and trans-zeatin riboside (tZ), isopentenyladenine (iP), and isopentenyladenosine (iPR) contents of the samples were determined using high performance liquid chromatography (HPLC). Hormone levels was measured using a high performance liquid chromatograph (LC-20AT) with a Hypersil BDS C18 chromatographic column. The flow rate was 1.0 mL·min−1.
RNA extraction and cDNA library construction
Total RNA was extracted from the ovules of the three developmental stages (35 DAP, 49 DAP, 63 DAP) of the C. henryi, namely ovule (O) at 35 DAP, fertile ovule (BO1), abortive ovule (SO1) at 49 DAP, fertile ovule (BO2), and aborted ovule (SO2) at 63 DAP. For the cDNA library construction and transcriptome sequencing, the total RNA was extracted using the Plant Total RNA Isolation Kit (Omega, China). We performed extraction with the Microto-Midi Total RNA Purification System according to the manufacturer’s instructions. RNA purity was checked using agarose gel electrophoresis and a NanoDrop ND1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) . The RNA integrity number (RIN) and rRNA ratio were used to assess RNA concentration and integrity using an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA).
First, qualified samples were taken as total RNA and the fragmentation buffer was poured into mRNA containing oligo (dT) magnetic beads, to fragment the samples. Second, the fragmented mRNA was used as a template. We applied six base random primers to synthesize the first strand of cDNA, and a mixture buffer, dNTPs (A, U, G, C), RNase H, and DNA polymerase I were used to synthesize the second strand of the cDNA chain. Following this step, the cDNA was purified using magnetic beads and eluted using the EB buffer. The fragments were end-repaired and A-tailed . Finally, by using PCR to purify and enrich double-stranded cDNA, a cDNA library was created. Completing the above steps indicated that the preparation of the entire library was complete.
Unigene assembly, annotation and analysis
On an Illumina NovaSeq 6000 Sequencing System, cDNA sequencing was completed using the paired-end sequencing method. We evaluated the measured raw read data. After the reads’ assembly, we obtained unigene samples of ovules, which were the basis for the gene structure annotation, functional annotation, and expression. Simultaneously, data generated through the RNA-Seq sequencing experiment were compared with the reference genome based on the species' reference genome and gene information. To determine the sequence orientation, we compared and annotated all detected unigene sequences with data samples that contained the NR, GO, KOG number, and KEGG databases [68,69,70].
Identification and analysis of differentially expressed genes
The differential expression analysis was performed using the EBSeq software . To analyze the differences in gene expression between different samples, we introduced the FPKM values. The Poisson distribution (parameters: fold change 2 and adjusted p-value 0. 001) is used in the DEG seq method. The Q-values were calculated after the P-values were corrected. We identified genes with larger than twofold differences and a Q-value ≤ 0.05 and filtered them as significantly differentially expressed genes to increase DEGs accuracy [72, 73]. Because of the limitations of using the database for unigene annotation, a keyword search was performed to identify other differentially expressed genes related to starch metabolism.
Quantitative real time PCR (qPCR) analysis
To validate the RNA-seq results, nine genes were chosen. The HiScript II Q RT Super Mix for qPCR (+ g DNA wiper) Kit was used to generate the first cDNA after extracting total RNA from five samples subjected to RNA-seq, following the manufacturer’s instructions. Primer 5.0 (Premier, Canada) was used to create gene-specific primers, and the primer sequences are listed in Supplementary Table S4. In a total reaction volume of 20 L, ChamQ Universal SYBR qPCR Master Mix (10 L; Vazyme, Nanjing, China) was mixed with gene-specific primers, sterilized water, and synthesized cDNA. Quantitative PCR was performed using a CFX96 Real-Time System (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions. Because GAPC1 expression is stable throughout ovule development, the internal control gene GAPC1 was used as the reference gene. To acquire relative mRNA expression data, the data were analyzed using the 2−∆∆Ct method. The qRT-PCR analysis was performed with three biological and three technical replicates.
Determination of starch metabolism-related enzyme activities
Likewise, at six developmental stages (35, 42, 49, 56, 63, and 70 DAP), the ovules were removed from the fruits and immediately stored in liquid nitrogen. We collected various samples of ovules (1 g) at each stage, added the pre-cooled extraction buffer, and homogenized the samples and centrifuged them in an ice bath. The supernatant was collected as the enzyme extract, which was used for the assessment of the α-amylase, beta-amylase, adenosine glucose diphosphate pyrophosphorylase (AGP), and starch synthase (SUSY) activities. The activities of AMY and beta-amylase were homogenized by adding distilled water and extracted according to the method described by Duke et al. . The activities of adenosine diphosphate glucose pyrophosphorylase (AGP) and starch synthase (SUSY) were determined using the Nakamura et al. Method . The activity of each enzyme was measured and thrice.
Availability of data and materials
The data of this project were available at NCBI Sequence Read Archive (SRA): PRJNA833559 (https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA833559).
Wilkinson LG, Tucker MR. An optimised clearing protocol for the quantitative assessment of sub-epidermal ovule tissues within whole cereal pistils. Plant Methods. 2017;13(1):67. https://doi.org/10.1186/s13007-017-0217-z.
Zhang Z, Yin Y, Liu F, Wu J, Fu T. Current situation and development countermeasures of Chinese rapeseed multifunction development and utilization. Chin J Oil Crop Sci. 2018;40(5):618. https://doi.org/10.7505/j.issn.1007-9084.
Khan SU, Yangmiao J, Liu S, Zhang K, Khan MHU, Zhai Y, Olalekan A, Fan C, Zhou Y. Genome-wide association studies in the genetic dissection of ovule number, seed number, and seed weight in Brassica napus L. Ind Crops Prod. 2019;142:111877. https://doi.org/10.1016/j.indcrop.2019.111877.
Zhang M, Li W, Feng J, Gong Z, Yao Y, Zheng C. Integrative transcriptomics and proteomics analysis constructs a new molecular model for ovule abortion in the female-sterile line of Pinus tabuliformis Carr. Plant Sci. 2020;294:110462. https://doi.org/10.1016/j.plantsci.2020.110462.
Thilges KA, Chamberlin MA, Albertsen MC, Horner HT. Microscopic characterization of a transposon-induced male-sterile, female-sterile mutant in Glycine max L. Int J Plant Sci. 2017;178(8):629–38. https://doi.org/10.1086/693857.
Mesejo C, Yuste R, Martínez-Fuentes A, Reig C, Iglesias DJ, Primo-Millo E, Agustí M. Self-pollination and parthenocarpic ability in developing ovaries of self-incompatible Clementine mandarins (Citrus clementina). Physiol Plant. 2013;148(1):87–96. https://doi.org/10.1111/j.1399-3054.2012.01697.x.
Downes R, Gladstones J. Physiology of growth and seed production in Lupinus angustifolius LI Effects on pod and seed set of controlled short duration high temperatures at flowering. Aust J Agric Res. 1984;35(4):493–9. https://doi.org/10.1071/ar9840493.
Tang W, Li R, Zhang Z, Baskin CC, Nan Z. Mulching affects seed set, provisioning, and offspring performance of Vicia unijuga (Fabaceae). Agron J. 2019;111(3):1341–57. https://doi.org/10.2134/agronj2018.09.0561.
Shaik SS, Obata T, Hebelstrup KH, Schwahn K, Fernie AR, Mateiu RV, Blennow A. Starch granule re-structuring by starch branching enzyme and glucan water dikinase modulation affects caryopsis physiology and metabolism. PLoS ONE. 2016;11(2):e0149613. https://doi.org/10.1371/journal.pone.0149613.
Wang J, Xu H, Zhu Y, Liu Q, Cai X. OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm. J Exp Bot. 2013;64(11):3453–66. https://doi.org/10.1093/jxb/ert187.
Stitt M, Zeeman SC. Starch turnover: pathways, regulation and role in growth. Curr Opin Plant Biol. 2012;15(3):282–92. https://doi.org/10.1016/j.pbi.2012.03.016.
Nazarian-Firouzabadi F, Visser RG. Potato starch synthases: functions and relationships. Biochem Biophys Rep. 2017;10:7–16. https://doi.org/10.1016/j.bbrep.2017.02.004.
Beck E, Ziegler P. Biosynthesis and degradation of starch in higher plants. Ann Rev Plant Biol. 1989;40(1):95–117. https://doi.org/10.1146/annurev.pp.40.060189.000523.
He HY, Tang ZP, Yang X, Fan WJ, Tan GN, Li LS, He XM. Research progress on potato starch synthesis and degradation. Biotechnol Bull. 2019;35(4):101. https://doi.org/10.13560/j.cnki.biotech.bull.1985.2018-0829.
Wang G, Shen Z, Ai L, Yu L, Song X, Xia Y. Research advance in amylolytic enzymes and hydrolysis products from amylose. Acta Agric Jiangxi. 2015;27(7):111–4. https://doi.org/10.19386/j.cnki.jxnyxb.2015.07.030.
Barro-Trastoy D, Dolores Gomez M, Tornero P, Perez-Amador MA. On the way to ovules: the hormonal regulation of ovule development. Crit Rev Plant Sci. 2020;39(5):431–56. https://doi.org/10.1080/07352689.2020.1820203.
Nole-Wilson S, Azhakanandam S, Franks RG. Polar auxin transport together with AINTEGUMENTA and REVOLUTA coordinate early Arabidopsis gynoecium development. Dev Biol. 2010;346(2):181–95. https://doi.org/10.1016/j.ydbio.2010.07.016.
Cheng CY, Kieber JJ. The role of cytokinin in ovule development in Arabidopsis. Plant Signal Behav. 2013;8(3):929–40. https://doi.org/10.4161/psb.23393.
Beppu K, Suehara T, Kataoka I. Embryo sac development and fruit set of’Satohnishiki’sweet cherry as affected by temperature, GA3 and paclobutrazol. J Jpn Soc Hortic Sci. 2001;70(2):157–62. https://doi.org/10.2503/jjshs.70.157.
Wang J, Liang H, Liu M. The relationship between endogenous hormone changes and embryo abortion during fruit development of Chinese jujube male sterile germplasm. J Plant Genet Resour. 2008;9(3):367–71. https://doi.org/10.2503/jjshs.70.157.
Wang H, Zhang S, Qu Y, Gao R, Xiao Y, Wang Z, Zhai R, Yang C, Xu L. Jasmonic acid and ethylene participate in the gibberellin-induced ovule programmed cell death process in Seedless Pear ‘1913’(Pyrus hybrid). Int J Mol Sci. 2021;22(18):9844. https://doi.org/10.3390/IJMS22189844.
Zhang CL, Zhang XH, Hang N, Jiang Y. Priminary study on analyses of nutrient ingredients in nuts of different chinquapin (Castanea henryi) cultivars. Subtrop Plant Sci. 2002;31(04):5. https://doi.org/10.3969/j.issn.1009-7791.2002.04.002.
Wu GL, Zhu ZJ, Qiu Q, Fan XM, Yuan DY. Transcriptome analysis reveals the regulatory networks of Cytokinin in promoting floral feminization in Castanea henryi. Int J Mol Sci. 2022;23(12):6389. https://doi.org/10.3390/IJMS23126389.
Liu Z, Yuan D, Zou F, Zhu Z, Wang G, Lu J. Effects of spraying borax at flowering stage on photosynthesis and empty shell rate in Castanea henryi. Acta Agric Univ Jiangxi. 2017;39(3):485–91. https://doi.org/10.13836/j.jjau.2017063.
Botta R, Vergano G, Me G, Vallania R. Floral biology and embryo development in chestnut (Castanea sativa Mill). HortScience. 1995;30(6):1283–6. https://doi.org/10.21273/HORTSCI.30.6.1283.
Gouhan D, Liangliu Z, Zhongwen X. Mechanism of formation of empty-shell chestnut for chestnut tree. J Fruit Sci (China) 1995. https://doi.org/10.13925/j.cnki.gsxb.1995.01.002.
Su J, Yao Y, Liu Y, Han Q, Zhang W. Function, structure and catalytic mechanism of sucrose phosphate synthase: a review. Sheng Wu Gong Cheng Xue Bao. 2021;37(6):1858–68. https://doi.org/10.13345/j.cjb.200743.
Olszewski N, Sun T-p, Gubler F. Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell. 2002;14(suppl 1):S61–80. https://doi.org/10.1105/tpc.010476.
Prathap V, Tyagi A. Correlation between expression and activity of ADP glucose pyrophosphorylase and starch synthase and their role in starch accumulation during grain filling under drought stress in rice. Plant Physiol Biochem. 2020;157:239–43. https://doi.org/10.1016/j.plaphy.2020.10.018.
Zhang S, Guo H, Irshad A, Xie Y, Zhao L, Xiong H, Gu J, Zhao S, Ding Y, Liu L. The synergistic effects of TaAGP. L-B1 and TaSSIVb-D mutations in wheat lead to alterations of gene expression patterns and starch content in grain development. PLoS ONE. 2019;14(10):e0223783. https://doi.org/10.1371/journal.pone.0223783.
Datta R, Chourey PS, Pring DR, Tang HV. Gene-expression analysis of sucrose-starch metabolism during pollen maturation in cytoplasmic male-sterile and fertile lines of sorghum. Sex Plant Reprod. 2001;14(3):127–34. https://doi.org/10.1007/s00497-001-0105-5.
Nanjo Y, Oka H, Ikarashi N, Kaneko K, Kitajima A, Mitsui T, Muñoz FJ, Rodríguez-López M, Baroja-Fernández E, Pozueta-Romero J. Rice plastidial N-glycosylated nucleotide pyrophosphatase/phosphodiesterase is transported from the ER-golgi to the chloroplast through the secretory pathway. Plant Cell. 2006;18(10):2582–92. https://doi.org/10.1105/tpc.105.039891.
Wang Z, Li W, Qi J, Shi P, Yin Y. Starch accumulation, activities of key enzyme and gene expression in starch synthesis of wheat endosperm with different starch contents. J Food Sci Technol. 2014;51(3):419–29. https://doi.org/10.1007/s13197-011-0520-z.
Lee SK, Jeon JS. Crucial role of inorganic pyrophosphate in integrating carbon metabolism from sucrose breakdown to starch synthesis in rice endosperm. Plant Sci. 2020;298:110572. https://doi.org/10.1016/j.plantsci.2020.110572.
Winter H, Huber SC. Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. Crit Rev Plant Sci. 2000;19(1):31–67. https://doi.org/10.1080/07352680091139178.
Guo J, Du M, Lu C, Wang B. NaCl improves reproduction by enhancing starch accumulation in the ovules of the euhalophyte Suaeda salsa. BMC Plant Biol. 2020;20(1):1–16. https://doi.org/10.1186/s12870-020-02468-3.
Wakizuka T, Nakajima T. Starch accumulation in integument with respect to ovule culture of petunia hybrida vilm. Jpn J Breed. 1978;28(1):63–70. https://doi.org/10.1270/jsbbs1951.28.6310.1270/jsbbs1951.28.63.
Yang XY, Liu GC, Lu DG, Qin SJ, Du GD. Anatomical study on the multi-ovule development and abortion of Hanfu apple. J Integr Agric. 2014;13(4):770–7. https://doi.org/10.1016/S2095-3119(13)60409-8.
Fadon E, Rodrigo J. Combining histochemical staining and image analysis to quantify starch in the ovary primordia of sweet cherry during winter dormancy. J Vis Exp. 2019;145:e58524. https://doi.org/10.3791/58524.
Liu J, Zhang H, Cheng Y, Wang J, Zhao Y, Geng W. Comparison of ultrastructure, pollen tube growth pattern and starch content in developing and abortive ovaries during the progamic phase in hazel. Front Plant Sci. 2014;5:528. https://doi.org/10.3389/fpls.2014.00528.
Singh R, Juliano BO. Free sugars in relation to starch accumulation in developing rice grain. Plant Physiol. 1977;59(3):417–21. https://doi.org/10.1104/PP.59.3.417.
Nakao Y, Shiozaki S, Ogata T, Kawase K, Horiuchi S. Changes in carbohydrate and water content with ovule growth of Ginkgo biloba L. J Hortic Sci Biotechnol. 1999;74(1):60–3. https://doi.org/10.1080/14620316.1999.11511072.
Bruun L. Histological and semi-quantitative approaches to in vitro cellular responses of ovule, embryo and endosperm in sugar beet, Beta vulgaris L. Sex Plant Reprod. 1991;4(1):64–72. https://doi.org/10.1007/BF00194574.
Fan X, Yuan D, Tang J, Tian X, Zhang L, Zou F, Tan X. Sporogenesis and gametogenesis in Chinese chinquapin (Castanea henryi (Skam) Rehder & Wilson) and their systematic implications. Trees. 2015;29(6):1713–23. https://doi.org/10.1007/s00468-015-1251-y.
Sokolov LN, Déjardin A, Kleczkowski LA. Sugars and light/dark exposure trigger differential regulation of ADP-glucose pyrophosphorylase genes in Arabidopsis thaliana (thale cress). Biochem J. 1998;336(3):681–7. https://doi.org/10.1042/bj3360681.
DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE. The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis. Plant J. 2006;45(1):1–16. https://doi.org/10.1111/j.1365-313X.2005.02592.x.
Monroe JD, Storm AR. The Arabidopsis β-amylase (BAM) gene family: diversity of form and function. Plant Sci. 2018;276:163–70. https://doi.org/10.1016/j.plantsci.2018.08.016.
Hou J, Zhang H, Liu J, Reid S, Liu T, Xu S, Tian Z, Sonnewald U, Song B, Xie C. Amylases StAmy23, StBAM1 and StBAM9 regulate cold-induced sweetening of potato tubers in distinct ways. J Exp Bot. 2017;68(9):2317–31. https://doi.org/10.1093/jxb/erx076.
Wiberley-Bradford AE, Busse JS, Bethke PC. Temperature-dependent regulation of sugar metabolism in wild-type and low-invertase transgenic chipping potatoes during and after cooling for low-temperature storage. Postharvest Biol Technol. 2016;115:60–71. https://doi.org/10.1016/j.postharvbio.2015.12.020.
Tran PT, Citovsky V. Receptor-like kinase BAM1 facilitates early movement of the Tobacco mosaic virus. Communs Biol. 2021;4(1):1–11. https://doi.org/10.1038/S42003-021-02041-0.
Miao H, Sun P, Miao Y, Liu J, Zhang J, Jia C, Wang J, Wang Z, Jin Z, Xu B. Genome-wide identification and expression analysis of the β-amylase genes strongly associated with fruit development, ripening, and abiotic stress response in two banana cultivars. Front Agric Sci Eng. 2017;3(4):346–56. https://doi.org/10.15302/J-FASE-2016127.
Zhang DL, Wang Y, Jia BC, Tian XQ, Chu J, Yin HB, Jameson PE, Chen S-H, Guo S-L. Genome-wide identification and expression analysis of the β-amylase gene family in chenopodium quinoa. DNA Cell Biol. 2021;40(7):936–48. https://doi.org/10.1089/DNA.2020.5911.
Chang Y, Yang J, Jiang L, Ren L, Zhou J. Chain length distribution of β-amylase treated potato starch and its effect on properties of starch nanoparticles obtained by nanoprecipitation. Starch-Stärke. 2019;71(9–10):1800321. https://doi.org/10.1002/star.201800321.
Cheng C, Xu X, Singer SD, Li J, Zhang H, Gao M, Wang L, Song J, Wang X. Effect of GA3 treatment on seed development and seed-related gene expression in grape. PLoS ONE. 2013;8(11):e80044. https://doi.org/10.1371/journal.pone.0080044.
Roxrud I, Lid SE, Fletcher JC, Schmidt ED, Opsahl-Sorteberg H-G. GASA4, one of the 14-member Arabidopsis GASA family of small polypeptides, regulates flowering and seed development. Plant Cell Physiol. 2007;48(3):471–83. https://doi.org/10.1093/pcp/pcm016.
Swain SM, Reid JB, Kamiya Y. Gibberellins are required for embryo growth and seed development in pea. Plant J. 1997;12(6):1329–38. https://doi.org/10.1046/j.1365-313x.1997.12061329.x.
Singh DP, Jermakow AM, Swain SM. Gibberellins are required for seed development and pollen tube growth in Arabidopsis. Plant Cell. 2002;14(12):3133–47. https://doi.org/10.1105/tpc.003046.
Matilla AJ. Auxin: Hormonal signal required for seed development and dormancy. Plants. 2020;9(6):705. https://doi.org/10.3390/plants9060705.
Marsch-Martínez N, de Folter S. Hormonal control of the development of the gynoecium. Curr Opin Plant Biol. 2016;29:104–14. https://doi.org/10.1016/j.pbi.2015.12.006.
Benková E, Michniewicz M, Sauer M, Teichmann T, Seifertová D, Jürgens G, Friml J. Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell. 2003;115(5):591–602. https://doi.org/10.1016/S0092-8674(03)00924-3.
Zhao T, Cheng L, Chen C-L, Wu Y-X, Wang H, Zhang J-Q, Zhu Y-F, Wang Y-X. Microstructural observation on pistil abortion of ‘Li Guang’apricot and transcriptome reveal the mechanism of endogenous hormones involved in pistil abortion. Sci Hortic. 2022;293:110749. https://doi.org/10.1016/J.SCIENTA.2021.110749.
Bencivenga S, Simonini S, Benková E, Colombo L. The transcription factors BEL1 and SPL are required for cytokinin and auxin signaling during ovule development in Arabidopsis. Plant Cell. 2012;24(7):2886–97. https://doi.org/10.1105/tpc.112.100164.
Li GR, Ji W, Wang G, Zhang JX, Wang YJ. An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes× wild Chinese Vitis species. In Vitro Cell Dev Biol Plant. 2014;50(1):110–20. https://doi.org/10.1007/s11627-013-9543-7.
Wang L, Yao W, Wang Y. The grape ubiquitin ligase VpRH2 is a negative regulator in response to ABA treatment. Planta. 2020;251(4):1–10. https://doi.org/10.1007/s00425-020-03382-6.
Wang J, Shi K, Lu W, Lu D. Effects of post-silking shading stress on enzymatic activities and phytohormone contents during grain development in spring maize. J Plant Growth Regul. 2021;40(3):1060–73. https://doi.org/10.1007/s00344-020-10164-7.
Fan X, Yuan D, Tian X, Zhu Z, Liu M, Cao H. Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in the flowers of Chinese chinquapin (Castanea henryi). J Agric Food Chem. 2017;65(47):10332–49. https://doi.org/10.1021/acs.jafc.7b03755.
Zhang L, Jia B, Tan X, Thammina CS, Long H, Liu M, Wen S, Song X, Cao H. Fatty acid profile and unigene-derived simple sequence repeat markers in tung tree (Vernicia fordii). PLoS ONE. 2014;9(8):e105298. https://doi.org/10.1371/journal.pone.0105298.
Kanehisa M. Toward understanding the origin and evolution of cellular organisms. Protein Sci. 2019;28(11):1947–51. https://doi.org/10.1002/pro.3715.
Kanehisa M, Furumichi M, Sato Y, Ishiguro-Watanabe M, Tanabe M. KEGG: integrating viruses and cellular organisms. Nucleic Acids Res. 2021;49(D1):D545–51. https://doi.org/10.1093/nar/gkaa970.
Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000;28(1):27–30. https://doi.org/10.1093/nar/28.1.27.
Leng N, Dawson JA, Thomson JA, Ruotti V, Rissman AI, Smits BM, Haag JD, Gould MN, Stewart RM, Kendziorski C. EBSeq: an empirical Bayes hierarchical model for inference in RNA-seq experiments. Bioinformatics. 2013;29(8):1035–43. https://doi.org/10.1093/bioinformatics/btt087.
Jin J, Zhang H, Kong L, Gao G, Luo J. PlantTFDB 3.0: a portal for the functional and evolutionary study of plant transcription factors. Nucleic Acids Res. 2014;42(D1):D1182–7. https://doi.org/10.1093/nar/gkt1016.
Wang L. Weighted multiple testing procedure for grouped hypotheses with k-FWER control. Comput Stat. 2019;34(2):885–909. https://doi.org/10.1007/s00180-018-0833-8.
Duke SH, Henson CA. A comparison of barley malt osmolyte concentrations and standard malt quality measurements as indicators of barley malt amylolytic enzyme activities. J Am Soc Brew Chem. 2009;67(4):206–16. https://doi.org/10.1094/ASBCJ-2009-0629-01.
Nakamura Y, Yuki K, Park SY, Ohya T. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol. 1989;30(6):833–9. https://doi.org/10.1093/oxfordjournals.pcp.a077813.
The authors are thankful Weiping Zhong and Xiao Hu from Central South University of Forestry and technology for helping with experiment work.
This research was funded by the Natural Science Foundation of Hunan Province (grant no. 2022JJ30997) and the Education Department of Hunan Province (20A530).
Ethics approval and consent to participate
The plant material used in this study has been identified by the Hunan Forest Tree Variety Approval Committee where Prof. Feng Zou from Central South University of Forestry and Technology (Changsha, Hunan Province, China) and Dr. Shixin Xiao from Central South University of Forestry and Technology (Changsha, Hunan Province, China) are particularly proficient in identification. and also confirms a voucher specimen of this material has been deposited in a publicly available herbarium (Forest Herbarium of the College of Forestry, Central South University of Forestry and Technology (https://www.cvh.ac.cn/ins/info.php?code=CSFI). The plant material of Chinese chinquapin (Castanea henryi) cultivar ‘Huali 4’ (XiangS-SC-SH-010–2015) and ‘Huali 2’ (XiangS-SC-SH-008–2015) were both approved by the Hunan Forestry Variety Approval Committee. All the supporting data are included in Additional files. All methods were carried out in accordance with relevant guidelines.
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Supplementary Figure S1. Comparison of ovule starch imaging under non-fluorescent (A-C) and fluorescent photographs (a-c). Supplementary Figure S2. Distribution of transcripts lengths.. Supplementary Figure S3. GO annotation of genes. Supplementary Figure S4. KEGG annotation of genes.
Supplementary Table S1. Statistical analysis of transcriptome data of 5 samples of 3 developmental stages of the C. henryi ovules. Supplementary Table S2. The length distribution of assembled transcripts. Supplementary Table S3. Highly and differentially expressed genes involved in starch metabolism in C. henryi ovules. Supplementary Table S4. Primers used in this study. Supplementary Table S5. Differentially expressed genes involved in hormonal regulation in C. henryi ovules.
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Qiu, Q., Tian, X., Wu, G. et al. Comparative analysis of the transcriptome during single-seed formation of Castanea henryi: regulation of starch metabolism and endogenous hormones. BMC Plant Biol 23, 90 (2023). https://doi.org/10.1186/s12870-023-04102-4