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Fig. 5 | BMC Plant Biology

Fig. 5

From: Comparative analysis of the transcriptome during single-seed formation of Castanea henryi: regulation of starch metabolism and endogenous hormones

Fig. 5

I Differentially expressed genes (DEGs) at three selected stages of ovule development. The blue and red columns indicate up-regulated and down-regulated DEGs, respectively. II Venn diagrams comparing significantly DEGs that overlap between different selected stages of the ovule development of the C. henryi. (A) O VS BO1, O VS BO2, O VS SO1 and O VS SO2. (B) BO1 VS BO2, SO1 VS SO2, SO1 VS BO1, and SO2 VS BO2. III Volcano plots of the transcriptome between O VS BO1 (A), O VS BO2 (B), O VS SO1 (C), O VS SO2 (D), BO1 VS BO2 (E), SO1 VS SO2 (F), SO1 VS BO1 (G), and SO2 VS BO2 (H). The statistical significance (log10 of p-value; Y-axis) was plotted against log2-fold change (X-axis). The center of the volcano represents a fold change of zero, while the sides indicate down-regulation and up-regulation. IV Validation of the expression patterns of differentially expressed genes selected from the RNA-seq analysis by qRT-PCR. The selected gene expression levels derived by the fragments per kilobase per million reads method are shown on the left Y-axis, while the relative gene expression levels determined by qRT-PCR are shown on the right Y-axis. O: ovule at 35 DAP; BO1: fertile ovule at 49 DAP; SO1: abortive ovule at 49 DAP; BO2: fertile ovule at 63 DAP; SO2: aborted ovule at 63 DAP. DAP: days after pollination

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