- Research article
- Open Access
Micronutrient content drives elementome variability amongst the Symbiodiniaceae
BMC Plant Biology volume 22, Article number: 184 (2022)
Elements are the basis of life on Earth, whereby organisms are essentially evolved chemical substances that dynamically interact with each other and their environment. Determining species elemental quotas (their elementome) is a key indicator for their success across environments with different resource availabilities. Elementomes remain undescribed for functionally diverse dinoflagellates within the family Symbiodiniaceae that includes coral endosymbionts. We used dry combustion and ICP-MS to assess whether Symbiodiniaceae (ten isolates spanning five genera Breviolum, Cladocopium, Durusdinium, Effrenium, Symbiodinium) maintained under long-term nutrient replete conditions have unique elementomes (six key macronutrients and nine micronutrients) that would reflect evolutionarily conserved preferential elemental acquisition. For three isolates we assessed how elevated temperature impacted their elementomes. Further, we tested whether Symbiodiniaceae conform to common stoichiometric hypotheses (e.g., the growth rate hypothesis) documented in other marine algae. This study considers whether Symbiodiniaceae isolates possess unique elementomes reflective of their natural ecologies, evolutionary histories, and resistance to environmental change.
Symbiodiniaceae isolates maintained under long-term luxury uptake conditions, all exhibited highly divergent elementomes from one another, driven primarily by differential content of micronutrients. All N:P and C:P ratios were below the Redfield ratio values, whereas C:N was close to the Redfield value. Elevated temperature resulted in a more homogenised elementome across isolates. The Family-level elementome was (C19.8N2.6 P1.0S18.8K0.7Ca0.1) · 1000 (Fe55.7Mn5.6Sr2.3Zn0.8Ni0.5Se0.3Cu0.2Mo0.1V0.04) mmol Phosphorous-1 versus (C25.4N3.1P1.0S23.1K0.9Ca0.4) · 1000 (Fe66.7Mn6.3Sr7.2Zn0.8Ni0.4Se0.2Cu0.2Mo0.2V0.05) mmol Phosphorous -1 at 27.4 ± 0.4 °C and 30.7 ± 0.01 °C, respectively. Symbiodiniaceae isolates tested here conformed to some, but not all, stoichiometric principles.
Elementomes for Symbiodiniaceae diverge from those reported for other marine algae, primarily via lower C:N:P and different micronutrient expressions. Long-term maintenance of Symbiodiniaceae isolates in culture under common nutrient replete conditions suggests isolates have evolutionary conserved preferential uptake for certain elements that allows these unique elementomes to be identified. Micronutrient content (normalised to phosphorous) commonly increased in the Symbiodiniaceae isolates in response to elevated temperature, potentially indicating a common elemental signature to warming.
Symbiodiniaceae are a taxonomically  and functionally [2, 3] diverse family of dinoflagellate microalgae considered a model organism to study phylogenetic and phenotypic responses to environmental change . Species within the Symbiodiniaceae thrive across a broad range of marine environments  underpinned by their diverse life histories [5,6,7]. Ubiquitous distribution and varied habitat preferences signifies how Symbiodiniaceae have evolved to diverse abiotic and biotic conditions that ultimately govern their functional diversity [3, 4]. With a few exceptions , Symbiodiniaceae are autotrophic, making them important primary producers in coastal ecosystems and essential symbiotic partners , particularly to scleractinian corals. Differences in photosynthetic performance have proved central in determining Symbiodiniaceae ecological success [2, 9]. Further, in identifying “ecotypes” adapted to particular factors regulating growth, such as light [2, 10,11,12], temperature [13,14,15], and CO2 . However, the complex interplay between taxonomically dependent and independent traits [2, 4] challenges identification of the most suitable traits defining Symbiodiniaceae functional diversity.
For higher plants [17,18,19] and phytoplankton [20,21,22], decades of research on intercellular elemental quotas (their elementome) has transformed knowledge on both phylogenetic  and functional diversity [23, 24]. Elementomes are predicted to be species-specific, reflecting the quantity and stoichiometry of elements required by an organism to survive and grow within any given environment. Elementomes are comprised of both macro- and micro-nutrients, and are determined by traits (unique set of characteristics ) of elemental acquisition, storage, and efflux [17, 20, 26, 27]. The ultimate set of structural and functional adaptations required by a species is predicted to result in an optimum elementome (at maximum fitness) – and therefore an “elemental phenotype”  governing and defining their biogeochemical niche . It has been postulated that elements which contribute a smaller proportion to cell biomass have greater variability in accumulation potential between species  and thus, we hypothesise could be good predictors of species-specific elementomes. In phytoplankton, cell size and elemental stoichiometry often respond in a predictable way to abiotic conditions because of biophysical rules that link growth rates, food web interactions, and biogeochemical cycling . Higher growth rates have been postulated to result in reduced nitrogen-to-phosphorous ratios due to the allocation of phosphorous to RNA synthesis required for protein synthesis (The growth rate hypothesis [30, 31]). Furthermore, the ‘specialised’ photosynthetic apparatus of microalgae, such as reaction centres, and photosynthetic pigment that contributes to functional ecotypes , may contribute to unique and predictable elemental content [32, 33]. Elementomes however are not only shaped by ecological function but also by environmental history. Thus, for Symbiodiniaceae maintained long-term in a common culturing environment, it is unknown whether resource requirements are evolutionarily conserved, and identifiable in unique elementomes.
Characterising marine phytoplankton elementomes have led to transformations in the understanding of ocean biogeochemistry [30, 34], as well as species functional responses to stress [35, 36]. However, comparatively little is known of Symbiodiniaceae elementomes and whether elemental phenotypes exist. Isolates within Symbiodiniaceae have immense phylogenetic diversity . They have well described differences in architecture  and photosynthesis functioning [38,39,40], including resource acquisition (e.g., light , carbon , and iron ) that would suggest they likely have evolutionary conserved elemental requirements [17, 26, 30]. Even the most commonly studied stoichiometric relationships, the Redfield ratios (carbon, nitrogen, phosphorus ratios; C:N:P [42, 43]), are rarely reported for Symbiodiniaceae. Only recently has fundamental information on trace metal requirements for Symbiodiniaceae species been reported [41, 44, 45], suggesting that Symbiodiniaceae trace metal requirements vary by ecological guild , light , and thermal history . Such studies have highlighted how a collective description of cellular elemental quotas can advance our understanding on Symbiodiniaceae physiology and stress responses. The extent of adherence to stoichiometric hypotheses as well as how evolutionarily conserved species requirements are for a broad suite of elements remains untested.
Here, we investigated six key macronutrients and nine micronutrients as part of the elementomes for ten Symbiodiniaceae isolates, spanning eight species. We established whether Symbiodiniaceae isolates have unique elementomes despite long-term maintenance under common nutrient replete conditions. Furthermore, we tested whether Symbiodiniaceae conform to the (i) growth rate hypothesis ; (ii) elemental-allometric scaling , (iii) photobiological ecotypes having unique elementomes , and (iv) whether elements that contribute less to biomass have greater variability between isolates  and thus are good predictors of species-specific elementomes. Finally, we assessed how elementomes of three isolates identified as having unique photobiological phenotypes change under a different thermal regime and hence plasticity beyond the fundamental biogeochemical niche.
Physiological diversity of Symbiodiniaceae isolates
Assessment of division rates, cell volume and photobiological traits confirmed that the 10 Symbiodiniaceae were physiologically diverse. Symbiodiniaceae division rates varied across isolates (F(20)= 3.669, p= 0.007; SI Table 1, Fig. 1a), where SCF082 exhibited the lowest mean (± SE) division rate of 0.19 ± 0.014 μ d−1, which differed from the two highest division rates for isolates SCF055-06 (0.24 ± 0.003 μ d−1) and CCMP2464 (0.23 ± 0.005 μ d−1) (p= 0.004, p= 0.039 respectively; SI Table 1, Fig. 1a). Division rates were within previously reported ranges for these isolates in nutrient replete medium [2, 37]. Cell volume varied across isolates (F(20)= 23.272, p< 0.001, SI Table 1), and within previously reported ranges [3, 37, 47], ranging from 599 ± 98.9 μm3 to 211 ± 38.3 μm3 for Symbiodinium (CCMP2548) and Durusdinium (SCF082) respectively (Fig. 1b). No commonalities were found across genera for the various photobiological traits assessed (Fig. 1c,e-h); however, two putative functional groups emerged when dynamic photochemical quenching (1-C) versus non-photochemical quenching (1-Q) were considered (Fig. 1d). Specifically, isolates CCMP3420, CCMP2548, CCMP2463, and CCMP2464 had different strategies of investing in 1-C versus 1-Q than compared to the other six isolates. Of the remaining photobiological parameters considered (σLHCII, τ1, τ2, τ2/PQOX), species-specific differences were only observed for τ2/PQOX (Fig. 1g; F(20)= 7.449, p< 0.001; SI Table 1), Fv/Fm (Fig. 1h; F(20)= 4.878, p< 0.002; SI Table 1) and σLHCII (Fig. 1c; F(20)= 10.631, p< 0.001; SI Table 1). Neither of the two electron transfer rates, τ1 or τ2 (Fig. 1e, f; p> 0.050; SI Table 1), differed between isolates.
The elementome of the ten Symbiodiniaceae isolates
Absolute Symbiodiniaceae elemental content – Across all isolates, macronutrient content ranged from 0.09 ± 0.02pg cell-1 for Ca to 31.48 ± 5.75pg cell-1 for C (SI Fig. 1). Micronutrients were within reported ranges for Symbiodiniaceae , with Fe the highest concentration (37.51 ± 6.85 fg cell-1), and V the lowest concentration (0.03 ± 0.01 fg cell-1) (SI Fig. 1). No notable trends of absolute elemental content were observed at genus level. However, at the isolate level, CCMP2464 exhibited the highest and most variable content for all micronutrients measured except for Cu (which was the highest concentration and most variable for CCMP2463). Thus, no phylogenetic trends were evident for any one element treated in isolation of others. Our results continue in the element normalised to phosphorus ratio format (E:P; see methods) unless otherwise noted, following Redfield ratios, and to allow us to test key stoichiometric hypotheses [21, 22, 27, 41, 44]. Variability in P between isolates (F(19)= 116.082, p< 0.001) can impact E:P, thus, the following results are valid for P-normalised data as per [21, 41]. Across isolates, all elements were in excess in the media relative to the cellular elemental content (SI Fig. 2), demonstrating nutrient replete growth conditions.
Isolate-specific differences of Symbiodiniaceae elementomes (27.4 °C) – PCA visualisation of the elementomes (E:P) revealed Symbiodiniaceae isolate-specific differences, but with some overlap predominately from larger variance amongst replicates for SCF058-04 and CCMP2548 (Fig. 2b, SI Tables 2, 3). All three Cladocopium isolates clustered together, driven by relatively high Ni:P and Se:P (SI Table 4). Collectively, the first two principal components accounted for 47.8% of the total Symbiodiniaceae elementome variance. The first principal component (PC1) accounted for 26.9% of the total elemental variance, with S:P, Ni:P, Sr:P and Fe:P contributing the largest loadings. ANOVA on the extracted ordination axes for PC1 confirmed differences between isolates (F(19)= 9.485, p< 0.001; SI Table 2). PC2 accounted for 20.9% of the total elementome variance, with V:P, Ca:P, Mn:P, and Zn:P contributing the largest loadings (SI Table 3). ANOVA on the extracted ordination axes for PC2 confirmed differences between isolates (F(19)= 3.706, p= 0.008; SI Table 2), with post-hoc Tukey identifying most differences were due to separation of CCMP2464 to the other isolates (SI Table 2). CCMP2464 exhibited higher Ca:P, Sr:P and V:P than most other isolates (Fig. 3), which explained its separation on the PCA. Collectively, the PCA demonstrates that several isolates can be resolved based on differences in certain E:P ratios; however, some isolates, e.g., SCF058-04, had large elementome variance across replicates that prohibited differentiation from other isolates here.
Symbiodiniaceae Redfield ratio (27.4 °C) – All Symbiodiniaceae isolates were characterised by C:N:P all well below the Redfield ratios of 106:16:1 (Fig. 2A). Both the largest (28:5:1; SCF055) and smallest (12:2:1; SCF055-06) C:N:P ratios were observed for isolates of Cladocopium. Differences were observed between isolates for N:P (F(19) = 2.449, p= 0.048) but not C:P (p> 0.050: SI Table 4). We also assessed C:N ratios as an elemental signature for cellular storage, where higher C:N suggests elevated storage . C:N was largest for CCMP2464 at 9.43, and lowest for SCF055-06 at 5.12 (Fig. 2A). Overall, C:N:P ratios remained relatively conserved across isolates.
Expanding beyond Redfield (27.4 °C) – Other macronutrient E:P ratios across all Symbiodiniaceae isolates, revealed S:P, K:P and Ca:P of 18.8, 0.7 and 0.1, respectively. As for C:N:P, these other macronutrient E:P ratios were highly variable between isolates, with no common trends observed across genera (Fig. 3). S:P and Ca:P were both significantly different across isolates (F(19)= 26.696, 8.771 respectively, p< 0.001; SI Table 4), but K:P remained relatively conserved (p> 0.050; SI Table 4). Micronutrient-to-P ratios for Ni:P, Se:P, Sr:P, Fe:P, V:P, and Cu:P were all significantly different across isolates (F in SI Table 4, p< 0.050) and, despite their low concentrations, micronutrient E:P ratios were strong drivers of isolate elementome separation, whereby micronutrient E:P ratios contributed to three of the four highest loadings in both PC1 and PC2 (see SI Table 3). Micronutrient E:P ratios were isolate-specific, but notably all Cladocopium isolates exhibited higher Ni:P content than other isolates. Isolate CCMP2464 exhibited higher and the most variable Sr:P, V:P, Mo:P, and Zn:P and CCMP2463 exhibited a higher Cu:P than all other isolates. These observations are consistent with those made on the absolute cellular concentrations of fg cell-1. Collective assessment of the elementome of Symbiodiniaceae under nutrient replete conditions, 27.4 ± 0.4 °C, and downwelling irradiance of 150.0 ± 4.05 μmol photons m-2s-1 defined the average elemental stoichiometry as: (C19.8N2.6 P1.0S18.8K0.7Ca0.1) · 1000 (Fe55.7Mn5.6Sr2.3Zn0.8Ni0.5Se0.3Cu0.2Mo0.1V0.04) mmol P-1. Overall, micronutrient E:P are greater contributors to isolate specific differences than macronutrient ratios for these Symbiodiniaceae isolates.
Symbiodiniaceae conformed to some, but not all stoichiometric hypotheses
Accumulation capacity for nutrients relative to proportion of cell biomass. Collectively across isolates, there was less than a seven-fold range in the maximum variability (maximum minimum-1) in macronutrient E:P ratios, and the contribution to cell biomass (median E:P) was always greatest for the macronutrients (SI Fig. 3). In general (except for C:P and S:P) maximum minimum-1 in E:P across all isolates declined with increasing contribution to cell biomass (median E:P; R2= 0.8, p < 0.001; SI Figs. 3 and 4), suggesting that Symbiodiniaceae generally conform to observations from other marine algae of decreasing variability in elemental content with increasing contribution to cell mass .
The growth rate hypothesis. N:P ratios have been suggested as a proxy for growth, with lower N:P denoting higher growth rates . However, the lowest and highest N:P was 1.82 and 5.09 for CCMP2463 and SCF055, respectively, and no correlation was observed between N:P ratios and growth rates across isolates (p> 0.050; SI Fig. 5).
Elemental allometric scaling. The absolute cellular content of any element did not correlate – and therefore did not allometrically scale – with cell volume (p> 0.050; SI Table 5). However, when carbon was plotted it was observed that SCF055-06 was an outlier, and if removed from the analysis, a strong significant positive correlation was observed (R2= 0.71, p= 0.005; SI Fig. 6). Variability in cell volume measured for SCF055-06 may explain why it did not correlate with C. With the exceptions of Mo and N that had a positive correlation (p< 0.002 and p= 0.043 respectively; SI Table 6), intracellular elemental content did not correlate with division rate (p> 0.050; SI Table 6). The RDA analysis conducted on E:P data did however identify cell volume as a significant predictor of Symbiodiniaceae elementomes (Table 2).
Photobiological ecotypes having unique elementomes. As discussed above, two putative functional groups of Symbiodiniaceae ecotypes were identified based on strategies of investing in 1-C versus 1-Q. The RDA revealed that investment into photochemical quenching (1-C) was a significant predictor of Symbiodiniaceae elementomes (Table 2). Isolates that had very rapid employment of 1-C over 1-Q (right-hand cluster; Fig.1d) had lower Ca:P and Sr:P and higher S:P, Ni:P and Se:P than the isolates with slower employment of 1-C over 1-Q (left-hand cluster; Fig. 1d, F values in SI Table 7, p< 0.010).
Homogenisation of Symbiodiniaceae isolate elementomes under elevated temperature
Three isolates (CCMP3420, SCF082, and CCMP2464) that are taxonomically diverse and demonstrated differences in their photobiological strategies (different strategies of investing in 1-C versus 1-Q; Fig. 1C) were grown under 30.7 °C to further assess how higher growth temperature influenced elementomes. Only SCF082 and CCMP3420 experienced a decline in Fv/Fm (p< 0.050; SI Table 8) and a slight reduction in division rates (p= 0.049; SI Table 9) under the higher temperature. Cell volume was unchanged across all isolates (p> 0.050; SI Table 9). At 30.7 °C, C:N:P was not significantly different to that at 27.4 °C (p> 0.050; SI Table 10), despite C:P being elevated, across isolates (Fig. 4A). C:N ratios increased across isolates at 30.7 °C (Fig. 4A) indicative of increased cellular storage . CCMP2464 had elevated Ca:P relative to the other two isolates (p< 0.05; SI Table 10) and ~3-fold increase in Cu:P at 30.7 °C compared to 27.4 °C (Fig. 4C). PCA revealed changes in elementomes resulting from growth temperature and driven by loadings contributing to PC2 (Fig. 4B), V:P, Ca:P, Mn:P, and Zn:P. Two-way ANOVA on the extracted ordination axes for PC2 confirmed differences across temperatures (F(12)= 5.854, p= 0.032; SI Tables 11, 12) but not isolates (p> 0.050). For PC1, there were differences in the extracted ordinations for each isolate (F(12)= 28.812, p< 0.001; SI Table 11), but no observed differences across temperature treatments (p> 0.050). PC1 accounted for 32.2% of the total elemental variance, while PC2 accounted for 24.0%. The resulting elemental stoichiometry of Symbiodiniaceae under elevated temperature (30.7 ± 0.4 °C) was: (C25.4N3.1P1.0S23.1K0.9Ca0.4) · 1000 (Fe66.7Mn6.3Sr7.2Zn0.8Ni0.4Se0.2Cu0.2Mo0.2V0.05) mmol P-1. Thus overall, under an elevated temperature a convergence (homogenisation) of isolate elementomes was observed (Fig. 4B).
Efforts to resolve the functional diversity of Symbiodiniaceae predominantly focus on their photosynthetic performance [2, 39, 49], thermal responses [15, 50, 51], and master traits of growth rate and cell size [2, 37] – only more recently have efforts begun to consider nutrient acquisition [3, 41, 52]. No study has yet determined the elementome of Symbiodiniaceae, to ascertain whether isolates have unique elemental content that would be an important functional trait of Symbiodiniaceae. In this study, the elementome was defined for Symbiodiniaceae maintained under long-term nutrient replete (luxury uptake) conditions to ascertain whether elementomes are evolutionarily conserved traits. We tested whether, under a different temperature regime “elemental phenotypes” exist for Symbiodiniaceae, and whether Symbiodiniaceae grown in replete medium adhere to postulated stoichiometric principles.
The isolate-specific elementomes of the Symbiodiniaceae
Elementomes could explain just under 50% of variation between the 10 Symbiodiniaceae isolates, and several isolates (although not all) could be uniquely identified based on their elemental signature. Macronutrient E:P was relatively conserved across isolates (except for S:P), with differences in micronutrient E:P primarily responsible for isolate-specific elementomes (for both E:P and absolute data), a phenomenon documented for other marine algae [21, 22]. There is generally less storage capacity for micronutrients making changes in stoichiometry more identifiable and our observations support recent suggestions that expansion beyond C:N:P is required to resolve a species’ biogeochemical niche . Some isolates, such as CCMP2464 had large variability in elementomes between biological replicates. A recent study advanced single-cell elementomic methods to allow quantification of the C content per cell in a Symbiodiniaceae culture, revealing significant heterogeneity within an isolate . Whether this corresponds to associated bacteria , viruses  or may even represent elemental phenotypes within a given isolate requires further study.
Despite common long-term (typically exceeding a decade) growth conditions, Symbiodiniaceae isolates have divergent elementomes. This suggests that isolate-specific preferential elemental acquisition is a conserved evolutionary trait . Differences in phylogeny did not resolve elementomes, but there were some commonalities in genera, for example, all Cladocopium exhibited elevated Ni:P. We did not observe elementome separation based on ecological guild . Instead, our data suggests that Symbiodiniaceae elementomes result from a complex interplay of phylogeny, host origin, environmental histories, and isolate-species traits. Examining more isolates is required to test this notion comprehensively given the broad ecological and geographic distribution of the Symbiodiniaceae. Further, this study used a common growth temperature for the ten Symbiodiniaceae isolates to allow comparison of their elementomes. However, the selected temperature is unlikely to reflects the optimal growth conditions for all isolates and was the rational to explore whether a different temperature induced a change in the isolate elementomes. Further work is needed to first establish optimal growth conditions for the Symbiodiniaceae isolates and then to assess how this impacts their elemental composition. Exploring how isolate-specific elementomes adjust under different environmental conditions, e.g., different temperatures, light intensities and nutrient conditions will provide knowledge on the plasticity of species elementomes and thus their biogeochemical niche .
Symbiodiniaceae confirm to some, but not all, proposed stoichiometric hypotheses
Some major elemental stoichiometric hypotheses that have been proposed for marine algae were considered for Symbiodiniaceae:
Symbiodiniaceae possess greater accumulation capacity for nutrients constituting smaller portions of cell biomass. Fogg & Thake  hypothesised greater accumulation capacity for nutrients that contribute a smaller proportion of cell biomass. We observed a significant correlation between the log-median E:P in biomass and the multiplicative range supporting this hypothesis and corroborating results from other marine phytoplankton studies [22, 34, 56]. This particularly held true for the micronutrients, with Fe:P and Mn:P contributing the greatest biomass and having the smallest multiplicative range (SI Fig. 3). For the macronutrients, the two highest E:P ratios (C:P and S:P) fell outside the 95% confidence interval for this correlation, highlighting how other processes besides allocation into cell biomass, including assimilation, and excretion , likely influence elemental acquisition.
The growth rate hypothesis is not universally applicable to the Symbiodiniaceae. It predicts a decrease in N:P with increased growth due to allocation of P to RNA synthesis required for protein synthesis [30, 57]. We did not find a decrease in N:P with increasing growth rate, adding to work that refutes the general applicability of the growth rate hypothesis , particularly for photosynthetic organisms , when P is not a limiting element  and can be stored when in excess as compounds such as polyphosphate .
Cell volumes are consistent predictors of Symbiodiniaceae elementomes. It is well understood that changes in growth rates impact nutrient requirements  and cell size has historically been used as a predictor for nutrient acquisition [60, 61]. Within our study, all but three isolates had similar growth rates, and thus, it is unsurprising that elemental concentrations generally did not (except Mo and N) correlate with growth rates. Cell volume was variable across isolates, but cellular C concentrations correlated with cell volume/size . Larger cells have greater biomass and storage capacity that can be utilised during luxury uptake conditions  and can have reduced solute leakage . However, allometric scaling does not always occur because growth depends on other factors such as nutrient source and organism physiology . Furthermore, organisms that are mixotrophic, which has been reported for Symbiodiniaceae , can use organic compounds as well as inorganic nutrients that would cause allometric scaling rules to break down . Biological substitution of elements will also cause allometric scaling on an element-by-element basis to break down. Indeed, our results did reveal that when the collective assessment of E:P (the elementome) was considered, cell volume was a good predictor of isolate elemental content. Such findings support the use of elemental ratios in resolving species functional traits since they account for fundamental stoichiometric relationships, and for species-specific bio-elemental substitution .
Elementomes of the Symbiodiniaceae reflect investment into photochemical (1-C) versus dynamic non-photochemical energy dissipation (1-Q). Dissipation of excitation energy by photochemical quenching (1-C) and cell volume were good predictors of isolate elementomes. These factors have previously been identified  as predictive factors of PSII electron transfer rates and in turn growth of Symbiodiniaceae, and thus identification of ecotypes. Our observations support the assertion that larger cells accommodate more photosynthetic machinery for light harvesting and utilisation  that allows greater photochemical quenching (1-C)  and we postulate that this is further reflected in Symbiodiniaceae nutrient acquisition and ultimate elementomes. Whilst no consistent E:P trace metal ratios were elevated in the isolates that had different strategies of investing into 1-C versus 1-Q, it seems plausible that isolate-specific differences in elemental uptake capacity, preferential elemental substitution  and differences in storage capacity  explain this result, but requires verification.
The elementome of Symbiodiniaceae grown under nutrient replete conditions
The elementome for ten Symbiodiniaceae isolates at 27.4°C was (C19.8N2.6 P1.0S18.8K0.7Ca0.1) · 1000 (Fe55.7Mn5.6Sr2.3Zn0.8Ni0.5Se0.3Cu0.2Mo0.1V0.04) mmol P-1. Thus, C:N:P is lower, and S:P and Fe:P higher, compared to elementomes for other marine algae [22, 66]. Caution is noted in direct comparisons between elementomes across studies, due to differences in normalisation (e.g. per cell, E:P or E:C), growth medium and abiotic conditions that can impact organism physiology, as well as the oxidation state of elements and their associated complexes. Even so, such comparisons are valuable in identifying major differences or similarities between isolates.
Symbiodiniaceae isolates examined here had C:N:P ratios below that of the Redfield ratios [42, 43], and lower than previous reports for cultured marine phytoplankton [21, 27, 66], including dinoflagellates  and in hospite Symbiodiniaceae . Values were however within ranges reported for marine algae cultured in nutrient replete media . Our cultures were sampled during exponential growth where there is greater allocation to P-rich assembly machinery and therefore a lower N:P ratio is expected . Our data corroborates research evidencing marine algae commonly exhibit broader C:N:P than the Redfield ratios [52, 56, 69, 70]. Compared to C:P and N:P, C:N (7.3) was similar to the expected Redfield value of 6.6. Stability in C:N versus C:P and N:P has been reported for other marine algae in situ  and in cultured nutrient replete conditions  and likely stems from both C and N comprising major constituents of cellular metabolic processes that account for a large proportion of cellular mass . In contrast, P generally contributes less biomass, is readily substituted [34, 71] and can be highly variable as several species can store excess amounts in large quantities [59, 72]. C:N appears to be well conserved across Symbiodiniaceae  and dinoflagellates  suggesting consistent essential cellular requirements of C and N.
All Symbiodiniaceae isolates examined here exhibited high S:P (18.8:1) compared to other marine phytoplankton [22, 27, 66]. Sulphur is required by algae for S-containing amino acids  and is commonly present in lipid fractions . Symbiodiniaceae also utilise S in forming the compound dimethylsulphoniopropionate in high concentrations compared to other microalgae [75, 76], potentially explaining the high S:P ratios in our study. Fe:P (55.7:1) across our isolates was elevated relative to previous values for Symbiodiniaceae (25-35:1 , ~5-25:1 ; see SI Fig. 7), as well as other dinoflagellates (~1.8-14:1 ). Higher Fe:P in our study likely reflects the high Fe medium content in IMK, with recent work  documenting increased intracellular Fe:P with higher Fe medium content. However, Rodriguez & Ho  recently demonstrated that the Fe:P of Fugacium kawagutii can vary 5-fold depending on the different light regimes at replete conditions (~13-69:1). Therefore, the magnitude of variation between our and previous Fe:P could also be attributed to light conditions (SI Fig. 7). The directionality of differences between the metal:P observed here and among the Symbiodiniaceae varied on an isolate- and metal- basis (; SI Fig. 7) and there were instances where metal:P measured in our study were less than conspecifics reared in low trace metal conditions. Overall, the within-isolate and within-species variability observed within our study and relative to others reflects the broad ranges of microalgal elemental phenotypes that exist ([41, 66], SI Fig 7).
Elevated temperature converges the elementome of Symbiodiniaceae isolates
All three isolates continued to grow at the elevated temperature, but in some cases with reduced growth rates and Fv/Fm, where the elementomes became more similar, primarily due to all isolates having increased uptake of most micronutrients. Reich et al.  recently found elevated trace metal content for a more thermally stable Symbiodiniaceae. Under elevated temperature, detoxification of oxygen radicals [15, 77, 78] and possible increased rates of cellular repair and respiration  require upregulation of metalloenzymes and thus a greater requirement of trace metals to meet the changing biogeochemical and cellular activities . Interestingly, Symbiodinium microadriaticum (CCMP2464) did not exhibit a reduction in growth rate or Fv/Fm but did result in higher Ca:P than the other two isolates. Recent work on other microalgae has revealed that increased cytosolic Ca2+ alleviated H2O2-induced oxidative stress by signalling a series of proteins  including the heat shock protein calmodulin that has been found to be conserved across Symbiodiniaceae clades . Calcium- and calcium/calmodulin-dependent protein kinases are also important in intercellular signalling [81, 82] that is possibly upregulated during the change in temperature regime and may have facilitated the physiological stability of S. microadriaticum at the elevated temperature. Such notions warrant more targeted investigation.
In summary, we show that evolutionary diverse Symbiodiniaceae retained in culture are characterised by very different elementomes, likely reflective of highly conserved isolate-specific nutrient acquisition and allocation strategies [3, 4, 83]. Further, Symbiodiniaceae elementomes (based on E:P) appear unique compared to other marine microalgae, and only adhere to some of the classical stoichiometric hypotheses derived from diverse microalgal taxa, including those elements (typically micronutrients) contributing least to cell biomass have greatest variability between isolates. Micronutrients appeared key in discriminating these Symbiodiniaceae isolate-specific differences in elemental composition and thus essential to resolving elemental phenotypes and environmental responses (to elevated temperature). Together, these outcomes suggest that quantifying elemental content, in particular micronutrients, likely enables greater understanding of how Symbiodiniaceae functioning is shaped by their environments, thereby potentially providing a powerful – yet unexplored - tool to better understand the impact of future environmental change.
Culturing and growth conditions
Ten Symbiodiniaceae isolates (defined by internal transcribed spacer two (ITS2) designation) were sub-cultured from a long-term laboratory stock (over a decade in nutrient replete medium except for PVB18B which was in culture for ca. 3 years) maintained under nutrient replete conditions at the University of Technology Sydney (UTS). Isolates were from five genera (Breviolum, Cladocopium, Durusdinium, Effrenium, Symbiodinium) and eight species originating from a range of geographic locations, host organisms, and lifestyle (see Table 1) [2, 15]. Genotyping (using the ITS2 region) of the Symbiodiniaceae cultures to re-verify identity was conducted as per [ref. 51].
Isolates were cultured in sterile 0.2 μm filtered artificial seawater enriched with Daigo’s IMK medium (Nihon Pharmaceutical, Tokyo, Japan)  to assess intercellular elemental quota differences across isolates when using a common nutrient replete culturing medium. A literature search using key terms “temperature”, “heat stress”, “thermal stress”, “thermal performance” and “stress response”, of “Symbiodiniaceae”, “Symbiodinium” and “Zooxanthellae” between 1/01/1980-to-present (23/05/2020) returned 1,379 papers. Of the studies that looked at ex situ culturing of Symbiodiniaceae and reported culturing conditions, 42% used nutrient replete medium (IMK, F2, ASP-8A), demonstrating that Symbiodiniaceae are frequently grown in luxury uptake conditions.
Initial elemental concentrations of the culture medium (filtered artificial seawater enriched with Daigo’s IMK medium) were determined by ICP-MS and are provided in SI Table 13. IMK medium also contained thiamine, biotin, and vitamin B12 (753, 6, 1 nM receptively), as well as 33 nM ethylenediaminetetraacetic acid (EDTA) to regulate elemental bioavailability [85, 41]. Culturing was undertaken in a clean bench (class 100 laminar flow) , and plasticware washed following the method of [ref. 44]. Cultures were maintained in 300 mL tissue culture flasks (Corning, NY, USA), grown in an incubator under a temperature (mean ± standard error, S.E.) of 27.4 ± 0.4 °C measured using a HOBO Pendant® data logger set to log every 30 min. Light was provided by fluorescent tubes (Philips cool white, 4000 K) with a downwelling irradiance of 150.0 ± 4.1 μmol photons m-1s1 on a 12:12h light:dark cycle, with intensity measured using a 4π LI-190SA Quantum Sensor (LI-COR, Lincoln, NE, USA). Four of the cultures (Symbiodinium microadriaticum CCMP2464, Effrenium voratum CCMP3420, Durusdinium trenchii SCF082 and Cladocopium goreaui SCF058-04) identified to have different photophysiological traits (see Fig. 1) were also grown under 30.7 ± 0.01 °C to assess the impact of a different temperature regime on their elementome. However, C. goreaui (SCF058-04) did not grow at this temperature and was therefore omitted from analysis.
Cultures (triplicates per isolate) were maintained in steady state growth phase through regular dilutions to prevent them from becoming optically thick or nutrient starved . All analyses were undertaken with cells in the exponential growth phase and each culture was grown for at least eight generations before sampling .
Growth and cell size
Growth was determined from cell quantification throughout culture monitoring to define the exponential division rate per day (μ, d-1) as per [ref. 11]. Cell quantification was determined using a haemocytometer (Neubauer Haemocytometer, Fisher Scientific, Loughborough, UK) as per [ref. 87]. Average cell volume for each isolate was determined from a minimum of 50 cells from images captured using NIS-Elements AR software (v.4.30.000) and an Eclipse Ni-U optical microscope coupled with a DS-Fi2 colour camera (Nikon, Tokyo, Japan). Images were processed on the NIS-Elements AR software to calculate spherical volume from cell diameter, of all cells present in each image. Elemental measurements were subsequently considered relative to both division rate and volume as both can influence nutrient acquisition .
On the days of elemental analyses, a 2 mL aliquot of culture was also collected for Light Induced Fluorescence Transient-Fast Repetition Rate fluorometry (LIFT-FRRf; Soliense Inc.) to obtain a suite of targeted photo-physiological parameters that have previously been identified as potential drivers of Symbiodiniaceae functional performance [2, 11]. To assess photosynthetic performance of the Symbiodiniaceae cultures, LIFT-FRRf parameters were selected that have previously been shown to govern functional differences amongst Symbiodiniaceae , including: maximum photochemical efficiency (Fv/Fm), absorption coefficient for the PSII light harvesting complex (σLHCII), electron transfer capacity from plastoquinone pigment PQA to plastocyanin (τ1), the plastoquinone re-oxidation rate (τ2), the plastoquinone re-oxidation rate normalised by the oxidised portion of the plastoquinone pool (τ2/PQOX), photochemical dissipation (1-C), and dynamic non-photochemical dissipation (1-Q). All parameters except for 1-C and 1-Q were collected in darkness. Values for 1-C and 1-Q were from 750 μmol photons m-2s-1. Calculation of all parameters are provided in SI.
Aliquots of 100 mL and 150 mL from each replicate were pelleted for each isolate for trace elemental analysis and for total C and N analysis, respectively. The supernatant was discarded, and the pellet washed three times in TRIS buffer prior to being freeze dried and weighed. ICP-MS analysis on the wash steps (see Method below) verified that three wash steps has cleaned the cells of excess medium and externally sorbed ions, and thus, reported values represent the intercellular metal quotas. At the time of elemental sample collection, 1 mL of culture was collected to determine cell density, and 2 mL for photobiological parameter characterisation (as described above). Samples (approximately 40 mg of pellet) were analysed for total C and N by dry combustion with a Trumac® CN-analyser (Leco® Castle Hill, Australia) following manufacturer methods for soil and plant material with a furnace temperature at 1200 °C and a soil calibration standard.
Remaining elements were analysed via ICP-MS/(MS). Approximate 12 mg of the pellet were digested with a mixture of 100 μL of HNO3 (67-69% w/w, Choice Analytical, Australia) and 100 μL of H2O2 (30-32% w/w, Seastar Chemicals, Canada) and incubated overnight. Samples were subsequently diluted to a final volume of 2 mL with Milli Q water (18.2 MΩ; Merk Millipore) and filtered using 0.2 μm syringe filters (Captiva Econofilters, Agilent Technologies, Australia). Sample containers were polypropylene to avoid adsorption effects. High purity ICP-MS standard calibration solutions for external calibration (Choice Analytical, Australia) were diluted in aqueous solution of 3.3% HNO3 and 1.5% H2O2. Procedural blank samples (TRIS) were also run to check for potential contamination in the methodological process and came back negligible (< 1%). Quantitative analysis of elements was carried out using flow injection analysis (FIA) employing an Agilent 1200-Series HPLC system coupled with an 8900-series ICP-MS/MS instrument (Agilent Technologies, Australia). The interface was equipped with s-lenses and Pt sampler and skimmer cones. The ICP-MS/MS instrument was operated in MS/MS mode using oxygen as cell gas (BOC, 99.995%, grade 4.5, Australia). Performance of the ICP-MS instrument was tuned daily with a solution containing 1μgL-1 Li, Y, Tl and Ce to optimise sensitivity. The elements S and P are commonly interfered in ICP-MS and were therefore analysed via mass shifting (31P➔31P16O and 32S➔32S16O). Targeted isotopes are listed in SI Table 14. Limits of detection were calculated according to the 3-sigma criterion.
Elemental concentrations were correlated against cell volume (arcsine transformed) and growth rate to examine for allometric and growth dependent elemental scaling. All subsequent analyses were conducted on element-to-phosphorous (E:P) ratios, following Redfield ratios  and because P is analysed simultaneously with the micronutrients [22, 27, 41, 43]. Macronutrient E:P is in mol:mol P-1 while micronutrient E:P is in mmol:mol P-1. E:P ratios were transformed to homogenise variance, and herein E:P refers to log-transformed data.
Six data analysis steps were employed: 1) Differences in traits (cell volume, division rate, σLHCII, τ1, τ2, τ2/PQOX, 1-C, 1-Q) between Symbiodiniaceae isolates was assessed by Analysis of Variance (ANOVA) with post-hoc Tukey, or Kruskal-Wallis test with post-hoc Dunn, depending on whether parametric test assumptions were fulfilled. Levene’s test was applied to assess for equal variance, and Shapiro-Wilk for normality combined with manual inspections of QQ-plots of the model residuals. Cell size, division rates, and photobiological traits were transformed to homogenise variance based on the bestNormalise package in R (Transformations in SI Table 15) ; 2) Elementomes amongst the 10 Symbiodiniaceae isolates was assessed using a principal component analysis (PCA) on zero-mean standardised E:P values, and the extracted ordination axes (PC1 and PC2) were compared across isolates using ANOVA and post-hoc Tukey. One replicate for SCF055-06 was omitted from the PCA since the C and N sample was lost. PCA loadings of E:P (visualised as vectors) were scaled to PCA eigenvalues; 3) Redundancy analysis (RDA) was next used to assess which Symbiodiniaceae traits are predictive of the elementome. Multicollinearity between traits was assessed through variance inflation scores ; 4) We then assessed whether variability in nutrient content decreased proportionately with cell mass . The multiplicative range of log E:P (maximum E:P variability) was determined by calculating the maximum x minimum-1 for each E:P over all Symbiodiniaceae isolates and temperature treatments and correlating this to log-median E:P (function of total contribution to cellular biomass) . Zero values were removed from the analysis; 5) Differences in E:P ratios across isolates were examined by ANOVA with post-hoc Tukey with Holm’s sequential correction; and finally, 6) The influence of an elevated temperature regime on the elementome of isolates CCMP2464, CCMP3420, and SCF082 was examined using PCA as described above. Differences in E:P ratios under temperature were compared with a two-way ANOVA with post-hoc Tukey. All data analysis was conducted in R (version 4), and code to reproduce these analyses are available online (https://github.com/nitschkematthew/Elementome_Symbiodiniaceae).
Availability of data and materials
Data generated or analysed during this study are included in this published article and its supplementary information files or can be obtained from the corresponding author on reasonable request. All raw sequence data are accessible under NCBI’s BioProject (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA812073).
LaJeunesse TC, Parkinson JE, Gabrielson PW, Jeong HJ, Reimer JD, Voolstra CR, et al. Systematic Revision of Symbiodiniaceae Highlights the Antiquity and Diversity of Coral Endosymbionts. Curr Biol. 2018;28(16):2570–80.e6.
Suggett DJ, Goyen S, Evenhuis C, Szabó M, Pettay DT, Warner ME, et al. Functional diversity of photobiological traits within the genus Symbiodinium appears to be governed by the interaction of cell size with cladal designation. New Phytol. 2015;208(2):370–81.
Wong JCY, Enríquez S, Baker DM. Towards a trait-based understanding of Symbiodiniaceae nutrient acquisition strategies. Coral Reefs. 2021;40(2):625–39.
Suggett DJ, Warner ME, Leggat W. Symbiotic Dinoflagellate Functional Diversity Mediates Coral Survival under Ecological Crisis. Trends Ecol Evol. 2017;32(10):735–45.
Cunning R, Yost DM, Guarinello ML, Putnam HM, Gates RD. Variability of Symbiodinium Communities in Waters, Sediments, and Corals of Thermally Distinct Reef Pools in American Samoa. PLoS One. 2015;29;10(12):e0145099.
Fujise L, Suggett DJ, Stat M, Kahlke T. Unlocking the phylogenetic diversity, primary habitats, and abundances of free-living Symbiodiniaceae on a coral reef. Molecular Ecology. 2020;30:343–60.
Nitschke MR, Davy SK, Cribb TH, Ward S. The effect of elevated temperature and substrate on free-living Symbiodinium cultures. Coral Reefs. 2015;34(1):161–71.
Jeong HJ, Du Yoo Y, Kang NS, Lim AS, Seong KA, Lee SY, et al. Heterotrophic feeding as a newly identified survival strategy of the dinoflagellate Symbiodinium. Proc Natl Acad Sci U S A. 2012;109(31):12604–9.
Camp EF, Edmondson J, Doheny A, Rumney J. Mangrove lagoons of the Great Barrier Reef support coral populations persisting under extreme environmental conditions. Mar Ecol. 2019;625:1–14.
Iglesias-Prieto R, Beltrán VH, LaJeunesse TC, Reyes-Bonilla H, Thomé PE. Different algal symbionts explain the vertical distribution of dominant reef corals in the eastern Pacific. Proc Biol Sci. 2004;271(1549):1757–63.
Hennige SJ, Suggett DJ, Warner ME, McDougall KE, Smith DJ. Photobiology of Symbiodinium revisited: bio-physical and bio-optical signatures. Coral Reefs. 2009;28(1):179–95.
Wangpraseurt D, Pernice M, Guagliardo P, Kilburn MR, Clode PL, Polerecky L, et al. Light microenvironment and single-cell gradients of carbon fixation in tissues of symbiont-bearing corals. ISME J. 2016;10(3):788–92.
Oakley CA, Schmidt GW, Hopkinson BM. Thermal responses of Symbiodinium photosynthetic carbon assimilation. Coral Reefs. 2014;33(2):501–12.
Díaz-Almeyda EM, Prada C, Ohdera AH, Moran H, Civitello DJ, Iglesias-Prieto R, et al. Intraspecific and interspecific variation in thermotolerance and photoacclimation in Symbiodinium dinoflagellates. Proc Biol Sci. 2017;284(1868).
Goyen S, Pernice M, Szabó M, Warner ME, Ralph PJ, Suggett DJ. A molecular physiology basis for functional diversity of hydrogen peroxide production amongst Symbiodinium spp. (Dinophyceae). Mar Biol. 2017;164(3):46.
Brading P, Warner ME, Davey P, Smith DJ, Achterberg EP, Suggett DJ. Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae). Limnol Oceanogr. 2011;56(3):927–38.
Penuelas J, Sardans J, Ogaya R, Estiarte M. Nutrient stoichiometric relations and biogeochemical niche in coexisting plant species: effect of simulated climate change. Pol J Ecol. 2008;56(4):613–22.
Göthlich L, Oschlies A. Phytoplankton niche generation by interspecific stoichiometric variation. Global Biogeochem Cycles. 2012;26(2).
Hu M, Peñuelas J, Sardans J, Sun Z, Wilson BJ, Huang J, et al. Stoichiometry patterns of plant organ N and P in coastal herbaceous wetlands along the East China Sea: implications for biogeochemical niche. Plant Soil. 2018;431(1):273–88.
Sunda WG, Huntsman SA. Processes regulating cellular metal accumulation and physiological effects: Phytoplankton as model systems. Sci Total Environ. 1998;219(2):165–81.
Quigg A, Finkel ZV, Irwin AJ, Rosenthal Y, Ho T-Y, Reinfelder JR, et al. The evolutionary inheritance of elemental stoichiometry in marine phytoplankton. Nature. 2003;425(6955):291–4.
Finkel ZV, Quigg A, Raven JA. Irradiance and the elemental stoichiometry of marine phytoplankton. Limnology and Oceanography. 2006;51(6):2690–701.
Bartrons M, Sardans J, Hoekman D, Peñuelas J. Trophic transfer from aquatic to terrestrial ecosystems: a test of the biogeochemical niche hypothesis. Ecosphere. 2018;9(7):e02338.
Urbina I, Sardans J, Beierkuhnlein C, Jentsch A, Backhaus S, Grant K, et al. Shifts in the elemental composition of plants during a very severe drought. Environ Exp Bot. 2015;111:63–73.
MacArthur RH. Geographical Ecology: Patterns in the Distribution of Species: Princeton University Press; 1984. p. 269.
Peñuelas J, Fernández-Martínez M, Ciais P, Jou D, Piao S, Obersteiner M, et al. The bioelements, the elementome, and the biogeochemical niche. Ecology. 2019;100(5):e02652.
Quigg A, Irwin AJ, Finkel ZV. Evolutionary inheritance of elemental stoichiometry in phytoplankton. Proc Biol Sci. 2011;278(1705):526–34.
Jeyasingh PD, Cothran RD, Tobler M. Testing the ecological consequences of evolutionary change using elements. Ecol Evol. 2014;4(4):528–38.
Fogg GE, Thake B. Algal Cultures and Phytoplankton Ecology. Univ of Wisconsin Press. 1987:269.
Sterner RW, Elser JJ. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere: Princeton University Press; 2002. p. 439.
Elser JJ, Acharya K, Kyle M, Cotner J, Makino W, Markow T, et al. Growth rate-stoichiometry couplings in diverse biota. Ecol Lett. 2003;6(10):936–43.
Raven JA, Evans MCW, Korb RE. The role of trace metals in photosynthetic electron transport in O2-evolving organisms. Photosynth Res. 1999;60(2/3):111–50.
Raven JA. The iron and molybdenum use efficiencies of plant growth with different energy, carbon and nitrogen sources. New Phytol. 1988;109(3):279–87.
Moore CM, Mills MM, Arrigo KR, Berman-Frank I, Bopp L, Boyd PW, et al. Processes and patterns of oceanic nutrient limitation. Nat Geosci. 2013;6(9):701–10.
Singh SP, Singh P. Effect of temperature and light on the growth of algae species: A review. Renewable Sustainable Energy Rev. 2015;50:431–44.
Diehl N, Karsten U, Bischof K. Impacts of combined temperature and salinity stress on the endemic Arctic brown seaweed Laminaria solidungula. J. Agardh. Polar Biol. 2020;43(6):647–56.
Ros M, Camp EF, Hughes DJ. Unlocking the black-box of inorganic carbon-uptake and utilization strategies among coral endosymbionts (Symbiodiniaceae). Limnology and Oceanography. 2020;65(8):1747–63.
Chang SS, Prézelin BB, Trench RK. Mechanisms of photoadaptation in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum. Mar Biol. 1983;76(3):219–29.
Iglesias-Prieto R, Trench RK. Acclimation and adaptation to irradiance in symbiotic dinoflagellates. I. Responses of the photosynthetic unit to changes in photon flux density. Mar Ecol Prog Ser. 1994;113:163–75.
Roberty S, Bailleul B, Berne N, Franck F, Cardol P. PSI Mehler reaction is the main alternative photosynthetic electron pathway in Symbiodinium sp., symbiotic dinoflagellates of cnidarians. New Phytol. 2014;204(1):81–91.
Reich HG, Rodriguez IB, LaJeunesse TC, Ho T-Y. Endosymbiotic dinoflagellates pump iron: differences in iron and other trace metal needs among the Symbiodiniaceae. Coral Reefs. 2020;39(4):915–27.
Redfield AC. Biological control of chemical factors in the environment. Am Sci. 1958;46(3):230A–221.
Redfield. On the proportions of organic derivatives in sea water and their relation to the composition of plankton. In James Johnstone Memorial Volume (Daniel, RJ, editor). 1934.
Rodriguez IB, Lin S, Ho J, Ho T-Y. Effects of Trace Metal Concentrations on the Growth of the Coral Endosymbiont Symbiodinium kawagutii. Front Microbiol. 2016;7:82.
Reich HG, Tu W-C, Rodriguez IB, Chou Y, Keister EF, Kemp DW, et al. Iron Availability Modulates the Response of Endosymbiotic Dinoflagellates to Heat Stress. J Phycol. 2021;57(1):3–13.
Rodriguez IB, Ho T-Y. Interactive effects of spectral quality and trace metal availability on the growth of Trichodesmium and Symbiodinium. PLoS One. 2017 30;12(11):e0188777.
Fujise L, Nitschke MR, Frommlet JC, Serôdio J, Woodcock S, Ralph PJ, et al. Cell Cycle Dynamics of Cultured Coral Endosymbiotic Microalgae (Symbiodinium) Across Different Types (Species) Under Alternate Light and Temperature Conditions. J Eukaryot Microbiol. 2018;65(4):505–17.
Szmant AM, Ferrer LM, FitzGerald LM. Nitrogen excretion and O:N ratios in reef corals: Evidence for conservation of nitrogen. Mar Biol. 1990;104(1):119–27.
Nitschke MR, Gardner SG, Goyen S, Fujise L. Utility of photochemical traits as diagnostics of thermal tolerance amongst great barrier reef corals. Front Mar Sci. 2018;5:45.
Robison JD, Warner ME. Differential impacts of photoacclimation and thermal stress on the photobiology of four different phylotypes of Symbiodinium (pyrrhophyta)1. J Phycol. 2006;42(3):568–79.
Camp EF, Kahlke T, Nitschke MR, Varkey D, Fisher NL, Fujise L, et al. Revealing changes in the microbiome of Symbiodiniaceae under thermal stress. Environ Microbiol. 2020;22(4):1294–309.
Carnicer O, Irwin AJ, Finkel ZV. Traits influence dinoflagellate C:N:P. Eur J Phycol. 2021;1–12.
Hofmann P, Clark A, Hoffmann P, Chatzinotas A, Harpole WS, Dunker S. Beyond nitrogen: phosphorus - estimating the minimum niche dimensionality for resource competition between phytoplankton. Ecol Lett. 2021;24(4):761–71.
Gonzalez de Vega R, Goyen S, Lockwood TE, Doble PA, Camp EF, Clases D. Characterisation of microplastics and unicellular algae in seawater by targeting carbon via single particle and single cell ICP-MS. Anal Chim Acta. 2021;7:338737.
Levin RA, Voolstra CR, Weynberg KD, van Oppen MJH. Evidence for a role of viruses in the thermal sensitivity of coral photosymbionts. ISME J. 2017;11(3):808–12.
Geider R, La Roche J. Redfield revisited: variability of C:N:P in marine microalgae and its biochemical basis. Eur J Phycol. 2002;37(1):1–17.
Elser JJ, Sterner RW, Gorokhova E, Fagan WF, Markow TA, Cotner JB, et al. Biological stoichiometry from genes to ecosystems. Ecol Lett. 2008;3(6):540–50.
Ågren GI. The C:N:P stoichiometry of autotrophs - theory and observations. Ecol Lett. 2004;7(3):185–91.
Norici A, Bazzoni AM, Pugnetti A, Raven JA, Giordano M. Impact of irradiance on the C allocation in the coastal marine diatom Skeletonema marinoi Sarno and Zingone. Plant Cell Environ. 2011;34(10):1666–77.
Taguchi S. Relationship between photosynthesis and cell size of marine diatoms1 2. J Phycol. 1976;12(2):185–9.
Tang EPY, Peters RH. The allometry of algal respiration. J Plankton Res. 1995;17(2):303–15.
Flynn KJ. Modelling multi-nutrient interactions in phytoplankton; balancing simplicity and realism. Prog Oceanogr. 2003;56(2):249–79.
Raven A, Finkel ZV, Irwin AJ. Picophytoplankton: bottom-up and top-down controls on ecology and evolution. Vie Milieu Paris. 2005;55(3-4):209–16.
Flynn KJ, Skibinski DOF, Lindemann C. Effects of growth rate, cell size, motion, and elemental stoichiometry on nutrient transport kinetics. PLoS Comput Biol. 2018;14(4):e1006118.
Finkel ZV, Beardall J, Flynn KJ, Quigg A, Rees TAV, Raven JA. Phytoplankton in a changing world: cell size and elemental stoichiometry. J Plankton Res. 2009;28;32(1):119–37.
Ho T-Y, Quigg A, Finkel ZV, Milligan AJ, Wyman K, Falkowski PG, et al. The elemental composition of some marine phytoplankton1. J Phycol. 2003;39(6):1145–59.
Blanckaert ACA, Reef R, Pandolfi JM, Lovelock CE. Variation in the elemental stoichiometry of the coral–zooxanthellae symbiosis. Coral Reefs. 2020;39(4):1071–9.
Geider RJ, La Roche J. The role of iron in phytoplankton photosynthesis, and the potential for iron-limitation of primary productivity in the sea. Photosynth Res. 1994;39(3):275–301.
Klausmeier CA, Litchman E, Daufresne T, Levin SA. Optimal nitrogen-to-phosphorus stoichiometry of phytoplankton. Nature. 2004;429(6988):171–4.
Moorthi SD, Schmitt JA, Ryabov A, Tsakalakis I, Blasius B, Prelle L, et al. Unifying ecological stoichiometry and metabolic theory to predict production and trophic transfer in a marine planktonic food web. Philos Trans R Soc Lond B Biol Sci. 2016;19;371(1694).
Godinot C, Ferrier-Pagés C, Grover R. Control of phosphate uptake by zooxanthellae and host cells in the scleractinian coral Stylophora pistillata. Limnol Oceanogr. 2009;54(5):1627–33.
Raven JA. Protein turnover and plant RNA and phosphorus requirements in relation to nitrogen fixation. Plant Sci. 2012;188-189:25–35.
Deane EM, O’Brien RW. Uptake of sulphate, taurine, cysteine and methionine by symbiotic and free-living dinoflagellates. Arch Microbiol. 1981;128(3):311–9.
Yuyama I, Higuchi T, Takei Y. Sulfur utilization of corals is enhanced by endosymbiotic algae. Biol Open. 2016;5(9):1299–304.
Raina J-B, Tapiolas D, Willis BL, Bourne DG. Coral-associated bacteria and their role in the biogeochemical cycling of sulfur. Appl Environ Microbiol. 2009;75(11):3492–501.
Jones GB. The Reef Sulphur Cycle: Influence on Climate and Ecosystem Services. In: Narchi N, Price LL, editors. Ethnobiology of Corals and Coral Reefs. Cham: Springer International Publishing; 2015. p. 27–57.
Krueger T, Becker S, Pontasch S, Dove S, Hoegh-Guldberg O, Leggat W, et al. Antioxidant plasticity and thermal sensitivity in four types of Symbiodinium sp. J Phycol. 2014;50(6):1035–47.
Levin RA, Beltran VH, Hill R, Kjelleberg S, McDougald D, Steinberg PD, et al. Sex, Scavengers, and Chaperones: Transcriptome Secrets of Divergent Symbiodinium Thermal Tolerances. Mol Biol Evol. 2016;33(11):3032.
Toseland A, Daines SJ, Clark JR, Kirkham A, Strauss J, Uhlig C, et al. The impact of temperature on marine phytoplankton resource allocation and metabolism. Nat Clim Chang. 2013;3(11):979–84.
Qiao T, Zhao Y, Zhong D-B, Yu X. Hydrogen peroxide and salinity stress act synergistically to enhance lipids production in microalga by regulating reactive oxygen species and calcium. Algal Research. 2021;53:102017.
Rosic N, Ling EYS, Chan C-KK, Lee HC, Kaniewska P, Edwards D, et al. Unfolding the secrets of coral-algal symbiosis. ISME J. 2015;9(4):844–56.
Nagamune K, Sibley LD. Comparative genomic and phylogenetic analyses of calcium ATPases and calcium-regulated proteins in the apicomplexa. Mol Biol Evol. 2006;23(8):1613–27.
Baker DM, Andras JP, Jordán-Garza AG, Fogel ML. Nitrate competition in a coral symbiosis varies with temperature among Symbiodinium clades. ISME J. 2013;7(6):1248–51.
Berges JA, Franklin DJ, Harrison PJ. Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades. J Phycol. 2001;37(6):1138–45.
Anderson MA, Morel FMM. The influence of aqueous iron chemistry on the uptake of iron by the coastal diatom Thalassiosira weissflogii1. Limnol Oceanogr. 1982;27(5):789–813.
International Atomic Energy Agency. Clean Laboratories and Clean Rooms for Analysis of Radionuclides and Trace Elements. International Atomic Energy Agency; 2003.
Goyen S, Camp EF, Fujise L, Lloyd A, Nitschke MR, LaJeunensse T, et al. Mass coral bleaching of P. versipora in Sydney Harbour driven by the 2015–2016 heatwave. Coral Reefs. 2019;38(4):815–30.
Peterson RA, Cavanaugh JE. Ordered quantile normalization: a semiparametric transformation built for the cross-validation era. J Appl Stat. 2020;47(13–15):2312–2.
Tabachnick BG, Fidell LS, Ullman JB. Using multivariate statistics, vol. 5: Pearson Boston; 2007.
Thank you to the Technical Staff at the University of Technology Sydney (UTS) for their support with sample processing, in particular Helen Price and Sue Fenech. We are also thankful for the support from Lucia Bennar in maintaining the long term Symbiodiniaceae stocks at UTS. Thanks, is also extended to Emily Scott for her assistance with the experiment and Nicole Dilernia for conducting a literature search on medium used within Symbiodiniaceae studies.
All experimentation was supported by an ARC Discovery Early Career Research Award (DE190100142) and University of Technology Sydney Chancellor’s Postdoctoral Research Fellowship awarded to EFC. The funding bodies were not involved in the study collection, analysis, interpretation of data, or the writing of the manuscript
Ethics approval and consent to participate
Consent for publication
The authors declare no conflicts of interest or competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Camp, E.F., Nitschke, M.R., Clases, D. et al. Micronutrient content drives elementome variability amongst the Symbiodiniaceae. BMC Plant Biol 22, 184 (2022). https://doi.org/10.1186/s12870-022-03512-0
- Elemental phenotyping
- Macronutrients; Micronutrients; Redfield ratio