Molecular dissection of Secale africanum chromosome 6Rafr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust
BMC Plant Biology volume 20, Article number: 134 (2020)
Introgression of chromatin from Secale species into common wheat has for decades been a successful strategy for controlling the wheat diseases. The wild Secale species, Secale africanum Stapf., is a valuable source for resistance to foliar disease of wheat. A wheat-S. africanum chromosome 6Rafr substitution line displayed resistance to both powdery mildew and stripe rust at the adult-plant stage.
Wheat-S. africanum chromosome 6Rafr deletion and translocation lines were produced and identified by sequential non-denaturing fluorescence in situ hybridization (ND-FISH) using multiple Oligo-based probes. Different ND-FISH patterns were observed between S. cereale 6R and S. africanum 6Rafr. With reference to the physical map of the draft genome sequence of rye inbred line Lo7, a comprehensive PCR marker analysis indicated that insertions and deletions had occurred by random exchange between chromosomes 6R and 6Rafr. A survey of the wheat- S. africanum 6Rafr lines for disease resistance indicated that a powdery mildew resistance gene(s) was present on the long arm of 6Rafr at FL0.85–1.00, and that a stripe rust resistance gene(s) was located in the terminal region of 6RafrS at FL0.95–1.00. The wheat-S. africanum 6Rafr introgression lines also displayed superior agronomic traits, indicating that the chromosome 6Rafr may have little linkage drag in the wheat background.
The combination of molecular and cytogenetic methods allowed to precisely identify the chromosome rearrangements in wheat- S. africanum 6Rafr substitution, deletion and translocation lines, and compare the structural difference between chromosomes 6R and 6Rafr. The wheat- S. africanum 6Rafr lines containing gene(s) for powdery mildew and stripe rust resistance could be used as novel germplasm for wheat breeding by chromosome engineering.
Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici (Bgt), and stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), are important diseases that occur in most wheat-growing regions. The areas affected by powdery mildew and stripe rust epidemics have increased under both rain-fed and irrigated high-input conditions in China, and also in many other countries in recent years [1, 2]. Cultivation of resistant cultivars is economical and environmentally friendly compared to chemical control. Considerable numbers of powdery mildew and stripe rust resistance genes have been identified and used in wheat breeding [3,4,5]. However, the effectiveness and commercial usefulness of these genes is often reduced due to emergence of new pathogen races. Race non-specific resistance and adult plant resistance (APR) genes, such as the multipathogen APR genes Lr34/Yr18/Sr57/Pm38  and Lr67/Yr46/Sr55/Pm46  are considered more durable than race specific resistance. The APR genes originated from the related species to wheat were also taken into consideration .
Cultivated rye (Secale cereale L.) has long been a valuable source of potentially useful genes for wheat improvement, which provided rich diversity for disease resistance gene introgression [8, 9]. Two of the most successful translocations were 1BL.1RS and 1AL.1RS, which carry powdery mildew genes Pm8 and Pm17, respectively. Further molecular clarification of Pm8 and Pm17 revealed that they were orthologous resistance genes with different evolutionary history [10, 11]. Friebe et al.  localized the powdery mildew resistance gene Pm20, which was derived from the chromosome 6RL of S. cereale cv. Prolific, which is currently still effective in China . Chromosome 6RL derived from S. cereale cultivars German White , JZHM , and Kustro [15, 16] also possess gene(s) for powdery mildew resistance. Another new powdery mildew resistance gene Pm56, which originated from Chinese native rye cultivar (cv.) QL , was recently mapped to the subtelomeric region of chromosome 6RS. However, the reports on the occurrence of stripe rust resistance genes derived from Secale species are limited. Chromosome 1RS in many wheat cultivars grown worldwide carry stripe rust resistance gene Yr9 ; however, that gene is no longer effective in most locations. Recently, a new stripe rust resistance gene Yr83 from 6R was reported , and chromosomes 1R and 4R with effective stripe rust resistance have been also incorporated into wheat in China [19, 20]. The potentially novel Yr genes on these chromosomes from different rye donors need further evaluation.
The wild species of Secale and related genera are considered potentially new sources of variation for wheat breeding purposes . In order to introduce novel disease resistance genes from the wild Secale species S. africanum into common wheat, we initiated a cross between a Triticum turgidum–S. africanum amphiploid and cultivated wheat [22, 23] and developed a large number of wheat-S. africanum introgression lines . Wheat-S. africanum substitution lines involving chromosomes 1Rafr , 2Rafr [26, 27], 5Rafr  and 6Rafr  with desirable agronomic traits were developed. These lines permitted the localization of genes on specific S. africanum chromosomes.
In the present study, we identified wheat - S. africanum chromosome 6Rafr substitution, deletion and translocation lines and physically localized gene(s) for powdery mildew and stripe rust resistance. The results also provided the opportunity to identify chromosomal rearrangements in the Secale genus by comparative molecular and cytogenetic analyses.
Identification of S. africanum 6Rafr and S. cereale 6R using ND-FISH
Non-denaturing FISH using probes Oligo-pSc119.2 and Oligo-pTa535 was conducted to characterize mitotic metaphase spreads of the wheat-S. cereale 6R (6D) line DS6R  and wheat-S. africanum 6Rafr (6D) line HH41 . FISH of line DS6R showed that telomeric region of chromosome 6RS had a single strong Oligo-pSc119.2 signal, whereas the 6RL had four prominent signal sites, two interstitial and two sub-telomeric (Fig. 1a). The S. africanum 6Rafr in HH41 had a strong Oligo-pSc119.2 signal at the telomeric region of the short arm, two signals in the terminal and sub-terminal regions of the long arm, and a faint hybridization site close to the centromere (Fig. 1b). FISH of hybrids between HH41 and DS6R revealed a length difference between 6Rafr and 6R (Fig. 1c); 6RL was about 15% longer than 6RafrL based on observations of 20 cells. Furthermore, a strong hybridization Oligo-pSc200 signal was present on 6RS, and two Oligo-pSc200 sites were located between the interstitial and sub-telomeric regions of 6RL, whereas chromosome 6Rafr was devoid of Oligo-pSc200 signals (Fig. 1d). Chromosomes 6Rafr and S. cereale 6R can thus be easily distinguished in the wheat background. The comparative FISH maps of the probes Oligo-pSc119.2 and Oligo-pSc200 on chromosomes 6R and 6Rafr are shown in Fig. 1e. It is probable that the presence or absence of pSc200-like sequences may cause the length differences between 6R and 6Rafr.
Identification of a 6Rafr deletion and translocations using FISH
A deletion line, translocation lines involving chromosome 6Rafr and wheat chromosomes identified among progenies of irradiated line HH41, and in progeny from the hybrid between HH41 and wheat line MY11 were screened by ND-FISH using Oligo-pSc119.2 and Oligo-pTa535. Four structural variants of 6Rafr were identified among 75 M4 lines derived from the irradiated HH41 line and named M26-B, r522-D, r1870-C and r600-E (Fig. 2). All four lines contained two copies of a 7B-2D reciprocal translocation. Line M26-B contained a pair of normal 6Rafr chromosomes substituting for chromosome 6D, but a reciprocal 7BS.2DS and 2DL.7BL was present in the wheat background (Fig. 2a). The FISH physical map of 6Rafr following hybridization with Oligo-pSc119.2 (Fig. 1e) showed that line r522-D had a deletion of the distal end of 6RafrS (named 6Rafr-1, Fig. 2b) and line r1870-C contained a deletion of the terminal end of 6RafrL (named 6Rafr-2, Fig. 2c). Line r600-E had a pair of modified 6Rafr chromosomes on which the strong terminal Oligo-pSc119.2 region was re-located to the end of the long arm, suggesting that a large inversion had occurred (named 6Rafr-3, Fig. 2d). In addition, a monosomic 6RafrS.6DL translocation (line H136, Fig. 2e), and a disomic 6DS.6RafrL translocation (line H86, Fig. 2f) were recovered among 286 plants derived from F4 families from the HH41 × MY11 cross. Lines disomic for 6RafrL (H536), monosomic for 6RafrS (H316), deleted for 6RafrS (H320), isochromosomal iso6RafrS (H297), iso6RafrL (H384), and iso6Rafr-2 L (H183) were also identified from these families (Fig. 3a-f). The FISH karyotypes of the broken and rearranged 6Rafr chromosome were further detected with a Oligo-(CAA)7 probe (Fig. 3g). All types of the 6Rafr deletion and translocation lines (Fig. 2 and Fig. 3) were confirmed by sequential FISH using the Secale-specific probe Oligo-1162 .
Physical localization of 6RafrS specific markers
Li et al.  localized 20 specific length amplified fragment (SLAF) markers to S. cereale cv. Kustral 6RKuS in a wheat background. These markers were tested on the 6Rafr deletion lines, but only 12 showed identical lengths of 6R-specific amplification products from M26-B, r522-D, r1870-C and r600-E. The 6RafrS.6DL line H136 showed amplification of the same 12 primer pairs, indicating that those markers were physically located in the S2-S3 region of 6RafrS (Fig. 1). Markers KU555 and KU226 on 6RKuS were amplified in line H86 with 6DS.6RafrL and are therefore located on 6RafrL. These results indicated that the centromeric regions were structurally rearranged between 6RKu and 6Rafr.
Markers GRM964  and 6VS-Bd1  were amplified in M26-B, r1870-C, r600-E and H136, but there was no amplification in r522-D and 6RafrL lines. Therefore these two markers are probably located in the telomeric S3 to S4 region of 6Rafr (Fig. 4 and Supplementary Table 1). In the assembled pesudo-molecules of S. cereale cv. Lo7, marker GRM964 was genetically mapped to the telomeric region of 2RS . Therefore, the marker and FISH data suggested that the telomeric Oligo-pSc119.2-rich region of S3 to S4 in 6RafrS (Fig. 1) was syntenic to the distal region of 2RLo7S.
Localization of molecular markers on chromosome 6RafrL
Qiu et al.  localized 190 markers specific for 6RKuL in Kustro rye. These markers were used to amplify DNA from the present 6Rafr derivatives. Only 66 of the 6RKuL-specific markers generated identical amplicons in 6Rafr(6D) substitution line HH41; the remaining 124 markers failed to amplify. Qiu et al.  physically located the 66 markers to three regions of 6RKuL; 35 (59%) of 60 markers in region I, 16 (35.6%) of 45 in region II, and 15 of 85 (17.6%) in region III showed specific amplification for 6RafrL. This suggested that regions close to the centromere in both 6RKu and 6Rafr are closely homologous, whereas the interstitial to sub-telomeric regions were more divergent. The greatest divergence occurred in the telomeric regions.
The DNA sequences of the 66 markers for 6RKuL were compared using the BLAST algorithm to the pseudo-molecules of 6RLo7, and 16 of these markers were physically located in the region covered by the pseudo-molecules of the Lo7 genome (Supplementary Table 1). To add more markers to the physical map of 6Rafr, 190 PLUG markers  and 321 CINAU markers  were assayed in amplified DNA from the 6Rafr deletion and translocation lines. The M26-B, r1870-C and H72 lines were also used for the amplification of the markers to determine the physical locations of these markers in chromosome 6RafrL. Thirty nine markers were physically mapped to L2 to L4 regions of 6RafrL (Supplementary Table 1 and Fig. 4). Three markers, KU-810, KU-10 and KU-340 mapped to the L4 to L5 regions of 6RafrL (Fig. 1).
Twenty four PLUG and CINAU markers corresponding to syntenic regions of wheat homoeologous chromosome groups 6, 3, 7 were physically located in regions corresponding to the pseudo-molecule in the draft Lo7 sequences (Supplementary Table 1). The results were consistent with the physical locations in the assembled genes in 6RLo7L  and homologous regions of the wheat genome . The distribution of pSc119.2 repeats was also predicted on the pseudo-molecule of 6RLo7 based on data from the B2DSC web server at http://mcgb.uestc.edu.cn/b2dsc . The pSc119.2 repeats were physically located on three regions of 71-72 Mb, 104-105 Mb and 128-129 Mb on chromosome 6RLo7 (Fig. 4). A comparison between the physical locations of the molecular and cytogenetic markers clearly indicated structural changes among 6RLo7L, 6RKuL and 6RafrL.
RNAseq of 6Rafr lines
In order to further characterize the deleted segment of 6RafrL in line r1870-C, a transcriptome analysis was performed to compare lines M26-B, r522-D, r1870-C and wheat parent MY11. A total of 42,572,656, 41,584,320, 44,426,580, and 44,932,204 reads were obtained for M26-B, r522-D, r1870-C and MY11, respectively. A sequence comparison revealed that about 80.59–81.66% reads were mapped on CS genome. Many fewer reads (34.9–36.8%) mapped to chromosome 6D in the 6Rafr lines than to 6D (81.9%) in MY11 indicating a lack of 6D in lines M26-B, r522-D, and r1870-C. The unigenes expressed in r26B, r522-D and r1870-C from the transcriptome data were also submitted to a search for genes annotated in pseudo-molecules of chromosome 6RLo7 . A total 2919 annotated transcripts covering 172 Mb specific for 6RLo7 were identified. The missing transcripts (with FPKM value = 0) were compared among the lines M26-B, r522-D and r1870-C (Fig. 5a) and 112 transcripts were missing in r1870-C. Based on the distribution of the non-expressed transcripts in the physical map of 6RLo7 (Fig. 5b), the corresponding regions of ~ 132–150 Mb of 6RLo7 were missing transcripts in r1870-C. Since the missing transcripts could be due to silencing or deletion of these regions, the results suggested that the deletion of r1870-C chromosome 6Rafr might be homologous to the genomic region of 132–150 Mb in 6RLo7 (Fig. 5).
Powdery mildew and stripe rust reactions
All 6Rafr lines were susceptible to Bgt isolates at the three-leaf seedling stage with infection types 3–4. The wheat parent MY11 was highly susceptible at all stages of growth. However, HH41 (IT = 0;, DS = 5) was fully resistant from the five-leaf stage and was considered to have adult-plant resistance (APR). Lines r1870-C and H136 were highly susceptible (IT = 4, DS = 85–90). The low infect type (IT = 0–1) and mean disease severity (DS = 18.6) of lines M26-B, r522-D, r600E, and H86 were characterised by necrotic areas without sporulation (Table 1, Fig. 6). This result indicated that the L4 to L5 segment (~FL 0.85–1.00) of S. africanum 6RafrL carried a gene(s) for powdery mildew resistance.
The 6Rafr lines were resistant when challenged with a Pst race mixture (CYR32, CYR33, and CYR34) in the field. In the field tests, HH41, M26-B, r1870-C, and r600-E were resistant, which had scores of IT 0–1, mean DS = 4.08 (0–10), and the average rAUDPC with mean 27.6%,(22.1 to 33.1%). The wheat parent MY11 and lines r522-D and H86 were susceptible with scores of IT = 4 and mean DS = 89.5 (80–95) (Table 1, Fig. 6b). It suggested that S. africanum 6RafrS carries a gene(s) for stripe rust resistance in the S3 to S4 segment (~FL 0.90–1.00).
Evaluation of agronomic traits
Agronomic traits of the wheat- S. africanum lines were evaluated under field conditions during the 2015 to 2018 growing seasons. As shown in Fig. 5c, lines HH41, M26-B, r600-E, r1870-C had increased spike length, plant height, and spikelet number relative to parent MY11 (Table 1). The data indicated that 6Rafr had little linkage drag on agronomic traits in the wheat background. However, line r522-D which lacked of the terminal pSc119.2-rich region of 6RafrS was significantly inferior in spike length and spikelet number. These results suggested that beneficial genes related to spike development might be located in the deleted segment of chromosome 6RafrS in r522-D. Line r1870-C displayed higher 1000-kernel weight than MY11 and the other 6Rafr lines, indicating that regions L4 to L5 in 6RafrL might have a negative effect on grain weight.
Molecular markers covering the entire rye genome and distributing evenly across different chromosome regions is helpful for detection of introgressed alien chromatin in wheat . Qiu et al.  developed 578 rye-specific PCR markers and Li et al.  produced 300 6RKuL-specific markers by using high-throughput SLAF-seq technology. The aforementioned markers were used for identification of chromosomes 1RKu to 7RKu and their specific arms in wheat. Bauer et al.  obtained a de novo assembled 1.68 Gb of gap-free sequence representing 21.26% of the 7.9 Gb genome of inbred winter rye line Lo7. The assembled pseudo-molecules of the Lo7 genome provide relative physical locations of rye-specific markers. Du et al.  developed 16 specific markers for a 6RKuL mini-chromosome and found that 14 of the 16 sequences had 91–100% similarity with nine scaffolds derived from chromosome 6RLo7 . In the present study, 66 SLAF markers specific for rye 6RKu were mapped on S. africanum 6Rafr. Of these markers 16 (24%) were physically located on the 6RLo7 pseudo-molecule maps. The PCR markers (Fig. 4) and RNAseq data (Fig. 5) blasted against the Lo7 genome sequences provided evidence for localization of deletion breakage points on the 6RafrL in line r1870-C. A complete, high-resolution genomic sequence of rye would be an invaluable resource for generating more chromosome-region-specific markers for targeting introgressed chromatin from different cultivated and wild rye genomes.
Well-conserved genome collinearity among cultivated and wild rye species is evident from the presence of large syntenic blocks that were disrupted by chromosomal rearrangements [31, 39, 40]. In the present study, a length differences between 6R and 6Rafr was evident in a hybrid between HH41 and DS6R. This length difference might be due to large accumulations of rye-specific repeats like the pSc200 sequence, in both the terminal region of 6RS and sub-terminal region of 6RL. Chromosome 6Rafr lacked all Oligo-pSc200 signals. A molecular marker analysis confirmed the complex segmental rearrangement from chromosome 6RS to 2RS in cultivated rye [40, 41], whereas S. africanum 6RafrS appears to have maintained close collinearity to the short arms of homoeologous group 6 chromosomes from the common ancestor of rye and wheat. Moreover, the peri-centromeric regions between 6RKuL and 6RafrL appeared well conserved, but the sub-telomeric regions showed higher structural variation. The greatest divergence of 6RKuL and 6RafrL was in the telomeric regions (Fig. 5). Data from comparative chromosome-specific PLUG and CINAU marker analysis suggested that the syntenic regions among wheat homoeologous group 6, and proximal sections of 6R and 6Rafr of rye were conserved, but the sub-terminal and telomeric regions of 6RL and 6RafrL were largely divergent.
Rye chromosomes with loss of telomeric C-bands or deletion of large segments have been described [42, 43]. Spontaneous redistribution and deletion of telomeric heterochromatin on rye chromosome 6R, and the complex structure of the distal region of 6RL, might have reduced the level of recovery of wheat-rye recombinants . Recently, Hao et al.  found that cultivated rye cv. QL chromosome 6R gave rise to different spontaneous deletions and translocations when it was transferred into wheat. Du et al.  identified a 6RKuL mini-chromosome addition line, MiA6RKuL, among the self-pollinated progeny of a wheat-rye 6RKuL monotelosomic addition line. In the present study, several types of 6Rafr telosomes were also observed among the offspring of the hybrid between HH41 and MY11 (Fig. 3). A strong pSc119.2 accumulated telomeric regions of 6RafrS was deleted in r522-D, whereas an inversion involving the distal region of 6RafrL appeared to have occurred in r600-E (Fig. 2).
Chromosome 6R from different cultivated rye genotypes contains disease resistance genes with potential for wheat improvement. 6RL from rye cv. Prolific [9, 10], JZHM , German White  and Kustro  have powdery mildew resistance genes. Hao et al.  reported powdery mildew resistance gene Pm56 on 6RS from rye cv. QL, and Li et al.  located stripe rust resistance gene Yr83 on 6RL. In our present study the L4 to L5 segment of chromosome 6RafrL conditioned adult plant resistance to powdery mildew, and 6RafrS carried a gene(s) for resistance to stripe rust. It is thus noteworthy that further introgression of chromatin from alternative cultivated and wild rye germplasm may provide additional disease resistance genes for wheat improvement.
In the present study, we developed and identified new wheat-S. africanum chromosome 6Rafr deletion and translocaton lines by using sequential in situ hybridization and molecular markers analysis. The large chromosome structural rearrangement between S. africanum 6Rafr and S. cereale 6R may be occurred during the evolution and speciation of Secale genus. The wheat- S. africanum 6Rafr lines displayed novel resistance to a powdery mildew and stripe rust at adult plant stages. We found that the gene(s) on the long arm of 6Rafr at FL0.85–1.00 were responsible for powdery mildew resistance, while the gene(s) was located in the terminal region of 6RafrS at FL0.95–1.00 were comply for stripe rust resistance. The wheat-S. africanum 6Rafr introgression lines with superior agronomic traits may serve as new bridging germplasm for wheat breeding.
The wheat-S. cereale 6R(6D) substitution line (DS6R) was provided by Dr. Ian Dundas, University of Adelaide, Australia. The S. cereale cv. QL, wheat cv. Mianyang 11 (MY11), wheat - S. africanum 6Rafr(6D) substitution line HH41 , and the T. durum-S. africanum amphiploid (YF) are maintained in the School of Life Science and Technology, University of Electronic Science and Technology of China. Wheat-S. africanum 6Rafr deletions were selected from M4 generation lines induced by 60Co γ-rays irradiation at the Institute of Biological and Nuclear Technology, Sichuan Academy of Agricultural Sciences in Chengdu. The wheat-S. africanum 6Rafr translocation and telosomic lines were obtained from the F4 populations derived from a cross of HH41 and MY11.
Fluorescence in situ hybridization (FISH)
Seedling roots for FISH were collected when they were 2-3 cm long and treated with nitrous oxide gas for 2 h under 1.0 MPa pressure. The treated roots were fixed in 90% acetic acid and washed before they were digested in the solution of 2% cellulase and 1% pectolyase (Yakult Pharmaceutical, Tokyo), which was referred the study of Lang et al. . The digested root sections were washed and the meristematic portions were mashed to form a cellular suspension in 100% acetic acid. The cell suspensions were dropped onto glass slides for chromosome preparation according to Han et al. . The oligo-nucleotide probes Oligo-pSc200, Oligo-pSc119.2 and Oligo-pTa535 were used for identifying the wheat chromosomes following the descriptions of Fu et al. . The production of the labeled oligonucleotide probes and the protocol of non-denaturing FISH (ND-FISH) employing synthesized probes was described by Tang et al. . After ND-FISH, the chromosome squashes for sequential FISH were washed twice to remove hybridization signals . The photography of FISH signals was performed under an fluorescence microscope (BX53, Olympus), and the images were processed according to Lang et al. .
Molecular marker analysis
DNA was extracted from young leaves using a sodium dodecyl sulfate (SDS) protocol . Primers from specific length amplified fragment sequencing (SLAF-seq) based PCR markers in rye  and 6R-specific markers [14, 15] were provided by Dr. Shulan Fu, Sichuan Agricultural University, Chengdu. PCR-based Landmark Unique Gene (PLUG) primers [34, 41], CINAU (Cytogenetics Institute, Nanjing Agricultural University, Nanjing, China) primers , and rye EST-derived SSR primers  were synthesized by Shanghai Invitrogen Biotechnology Co. Ltd. Polymerase chain reaction amplification, restriction enzyme-digestion and electrophoresis were as described by Li et al. . Markers for physical location in chromosomes were obtained by searching the database from the International Wheat Genome Sequencing Consortium whole genome assembly ref. v1.0 , and the whole-genome shotgun sequencing assembly of inbred winter rye line Lo7 .
RNA-seq and transcriptomic analysis
Leaves of two-week-old seedlings were harvested for RNA extraction and sequencing. Total RNA was isolated and quality tested as previously described . The cDNA library construction and sequencing were performed with a HiSeq2000 analyser (Illumina) according to the manufacturer’s instructions. Transcriptome assembly was accomplished using Trinity  with default parameters. All unigene sequences from RNA-seq that had the highest identity to the wheat and rye chromosome homologous group 6 were selected for differential expression analysis. The reference genome sequence of Chinese spring (CS) was downloaded from IWGSC (http://www.wheatgenome.org/) . The draft genome sequences of rye was from GrainGenes (https://wheat.pw.usda.gov/cgi-bin/seqserve/blast_rye.cgi) . Four RNA-seq datasets from wheat-6Rafr lines and MY11 wheat were compared for genome and gene expression analyses.
Powdery mildew and stripe rust reactions.
Lines were evaluated at the seedling to adult plant stages in a greenhouse at 22 °C and photoperiod of 14 h of light per day. A local mixed Bgt isolates was used for inoculation. Four-leaf stage was found to be suitable for measuring APR, and the APR response of each plant was recorded on infection type (IT) and disease severity (DS) scale at 10, 15 and 20 days post-inoculation Molher et al. . The two superposed leaves below flag leaf showing the highest infestation in the parent MY11 were examined as control.
Stripe rust reactions were observed in field-grown plants at the Sichuan Academy of Agricultural Science Experimental Station. Ten plants were grown 1 m in rows with a 25 cm spacing between rows. Bread wheat MY11 planted on both sides of each experimental row served as an inoculum spreader and susceptible control after inoculation with races CYR32, 33 and 34. Reactions evaluated at the heading and grain-filling stages were scored for adult plant responses based on the infection types (IT) and disease severity (DS), the area under the disease progress curve (AUDPC) and relative AUDPC (rAUDPC) according to the report of Yuan et al. .
Evaluation of agronomic traits.
Each line was evaluated for plant height (cm), spike length (cm), spikelets per spike, and 1000-kernel weight (g). The significance of differences in agronomic traits among the lines was assessed using SPSS 16.0 (IBM, Armonk, NY, USA).
Availability of data and materials
The datasets of raw Illumina sequences generated in the current study were deposited to the National Center for Biotechnology Information (NCBI) and can be accessed in the Short Read Archive (SRA) database (https://www.ncbi.nlm.nih.gov/sra) as accession number PRJNA610716. All plant materials analysed during this study are available from the corresponding author by request.
Area under the disease progress curve
Blumeria graminis f. sp. tritici
Fluorescence in situ hybridization
non-denaturing fluorescence in situ hybridization
Puccinia striiformis f. sp. tritici
Specific length amplified fragment
Sodium dodecyl sulfate
Morgounov A, Tufan HA, Sharma R, Akin B, Bagci A, Braun HJ, Kaya Y, Keser M, Payne TS, Sonder K, McIntosh R. Global incidence of wheat rusts and powdery mildew during 1969-2010 and durability of resistance of winter wheat variety Bezostaya 1. Eur J Plant Pathol. 2012;132:323–40.
Schwessinger B. Fundamental wheat stripe rust research in the 21st century. New Phytol. 2017;213:1625–31.
Tan C, Li G, Cowger C, Carver BF, Xu X. Characterization of Pm63, a powdery mildew resistance gene in Iranian landrace PI 628024. Theor Appl Genet. 2019;132:1137–44.
Nsabiyera V, Bariana HS, Qureshi N, Wong D, Hayden MJ, Bansal UK. Characterisation and mapping of adult plant stripe rust resistance in wheat accession Aus27284. Theor Appl Genet. 2018;131:1459–67.
McIntosh RA, Dubcovsky J, Rogers WJ, Xia XC, Raupp WJ. Catalogue of gene symbols for wheat: 2018-19 supplement. Ann Wheat Newsl. 2018;64:73–93.
Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, et al. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science. 2009;323:1360–3.
Moore JW, Herrera-Foessel S, Lan C, et al. A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nat Genet. 2015;47:1494–8.
Ellis JG, Lagudah ES, Spielmeyer W, Dodds PN. The past, present and future of breeding rust resistant wheat. Front Plant Sci. 2014;5:641.
Mago R, Zhang P, Vautrin S, Šimková H, Bansal U, Luo MC, Rouse M, et al. The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus. Nat Plants. 2015;1:15186.
Hurni S, Brunner S, Buchmann G, Herren G, Jordan T, Krukowski P, et al. Rye Pm8 and wheat Pm3 are orthologous genes and show evolutionary conservation of resistance function against powdery mildew. Plant J. 2013;76:957–69.
Singh SP, Hurni S, Ruinelli M, Brunner S, Sanchez-Martin J, Krukowski P, et al. Evolutionary divergence of the rye Pm17 and Pm8 resistance genes reveals ancient diversity. Plant Mol Biol. 2018;98:249–60.
Friebe B, Heun M, Tuleen N, Zeller FJ, Gill BS. Cytogenetically monitored transfer of powdery mildew resistance from rye into wheat. Crop Sci. 1994;34:621–5.
An D, Zheng Q, Luo Q, Ma P, Zhang H, Li L, Han F, Xu H, Zhang X, Zhou Y. Molecular cytogenetic identification of a new wheat-rye 6R chromosome disomic addition line with powdery mildew resistance. PLoS One. 2015;10:e0134534.
Wang D, Zhuang LF, Sun L, Feng YG, Pei ZY, Qi ZJ. Allocation of a powdery mildew resistance locus to the chromosome arm 6RL of Secale cereale L. cv. ‘Jingzhouheimai’. Euphytica. 2010;176:157–66.
Li M, Tang ZX, Qiu L, Wang YY, Tang SY, Fu SL. Identification and physical mapping of new PCR-based markers specific for the long arm of rye (Secale cereale L.) chromosome 6. J Genet Genomics. 2016;43:199–206.
Du H, Tang Z, Duan Q, Tang S, Fu S. Using the 6RLKu minichromosome of rye (Secale cereale L.) to create wheat-rye 6D/6RLKu small segment translocation lines with powdery mildew resistance. Int J Mol Sci. 2018;19:3933.
Hao M, Liu M, Luo J, Fan C, Yi Y, Zhang L, Yuan Z, Ning S, Zheng Y, Liu D. Introgression of powdery mildew resistance gene Pm56 on rye chromosome arm 6RS into wheat. Front Plant Sci. 2018;9:1040.
Li J, Dundas I, Dong C, Li G, Trethowan R, Yang Z, Hoxha S, Zhang P. Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. Theor Appl Genet. 2020. https://doi.org/10.1007/s00122-020-03534-y.
Wang C, Zheng Q, Li L, Niu Y, Wang H, Li B, Zhang X, Xu Y, An D. Molecular cytogenetic characterization of a new T2BL.1RS wheat-rye chromosome translocation line resistant to stripe rust and powdery mildew. Plant Dis. 2009;93:124–9.
An D, Ma P, Zheng Q, Fu S, Li L, Han F, Han G, Wang J, Xu Y, Jin Y, Luo Q, Zhang X. Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot. Theor Appl Genet. 2019;132:257–72.
Tang ZX, Ross K, Ren ZL, Yang ZJ, Zhang HY, Chikmawati T. Miftahudin, Gustafson JP. Wealth of wild species: role in plant genome elucidation and improvement - Secale. In: Kole C, editor. Wild crop relatives: genomic and breeding resources cereals. Switzerland.: Springer; 2011. p. 367–95.
Yang ZJ, Li GR, Ren ZL. Identification of amphiploid between Triticum durum cv. Ailanmai native to Sichuan, China and Secale africanum. Wheat Inf Serv. 2000;91:20–4.
Yang ZJ, Li GR, Ren ZL. Identification of Triticum durum–Secale africanum amphiploid and its crossability with common wheat. J Genet Breed. 2001;55:45–50.
Yang ZJ, Li GR, Jia JQ, Zeng X, Lei MP, Zeng ZX, Zhang T, Ren ZL. Molecular cytogenetic characterization of wheat-Secale africanum amphiploids and derived introgression lines with stripe rust resistance. Euphytica. 2009;167:197–202.
Lei MP, Li GR, Liu C, Yang ZJ. Characterization of new wheat- Secale africanum derivatives reveals evolutionary aspects of chromosome 1R in rye. Genome. 2012;55:765–74.
Lei MP, Li GR, Zhang SF, Liu C, Yang ZJ. Molecular cytogenetic characterization of a new wheat Secale africanum 2Ra (2D) substitution line for resistance to stripe rust. J Genet. 2011;90:283–7.
Lei MP, Li GR, Zhou L, Li CH, Liu C, Yang ZJ. Identification of wheat-Secale africanum chromosome 2Rafr introgression lines with novel disease resistance and agronomic characteristics. Euphytica. 2013;194:197–205.
Li G, Gao D, La S, Wang H, Li J, He W, Yang E, Yang Z. Characterization of wheat-Secale africanum chromosome 5Ra derivatives carrying Secale specific genes for grain hardness. Planta. 2016;243:1203–12.
Li G, Zhang H, Zhou L, Gao D, Lei M, Zhang J, Yang Z. Molecular characterization of Sec2 loci in wheat-Secale africanum derivatives demonstrates genomic divergence of Secale species. Int J Mol Sci. 2015;16:8324–36.
Fu SL, Chen L, Wang YY, Li M, Yang ZJ, Qiu L, Yan BJ, Ren ZL, Tang ZX. Oligonucleotide probes for ND-FISH analysis to identify rye and wheat chromosomes. Sci Rep. 2015;5:10552.
Martis MM, Zhou R, Haseneyer G, Schmutzer T, Vrána J, Kubaláková M, König S, Kugler KG, Scholz U, Hackauf B, Korzun V, Schön CC, Dolezel J, Bauer E, Mayer KF, Stein N. Reticulate evolution of the rye genome. Plant Cell. 2013;25:3685–98.
He H, Zhu S, Sun W, Gao D, Bie T. Efficient development of Haynaldia villosa chromosome 6VS-specific DNA markers using a CISP-IS strategy. Plant Breed. 2013;132:290–4.
Qiu L, Tang ZX, Li M, Fu SL. Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.). Genome. 2016;59:159–65.
Ishikawa G, Nakamura T, Ashida T, Saito M, Nasuda S, Endo T, Wu J, Matsumoto T. Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers. Theor Appl Genet. 2009;118:499–514.
Zhang X, Wei X, Xiao J, Yuan C, Wu Y, et al. Whole genome development of intron targeting (IT) markers specific for Dasypyrum villosum chromosomes based on next-generation sequencing technology. Mol Breeding. 2017;37:115.
Bauer E, Schmutzer T, Barilar I, Mascher M, Gundlach H, Martis MM, Twardziok SO, Hackauf B, Gordillo A, Wilde P, et al. Towards a whole-genome sequence for rye (Secale cereale L.). Plant J. 2017;89:853–69.
Appels R, Eversole K, Feuillet C, Keller B, Rogers J, Stein N, Pozniak CJ, Choulet F, Distelfeld A, Poland J, et al. Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science. 2018;361:eaar7191.
Lang T, Li G, Wang H, Yu Z, Chen Q, Yang E, Fu S, Tang Z, Yang Z. Physical location of tandem repeats in the wheat genome and application for chromosome identification. Planta. 2019;249:663–75.
Naranjo T, Fernandez-Rueda P. Homoeology of rye chromosome arms to wheat. Theor Appl Genet. 1991;82:577–86.
Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD. Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet. 1993;85:673–80.
Li J, Endo TR, Saito M, Ishikawa G, Nakamura T, Nasuda S. Homoeologous relationship of rye chromosome arms as detected with wheat PLUG markers. Chromosoma. 2013;122:555–64.
Gustafson JP, Lukaszewski AJ, Bennett MD. Somatic deletion and redistribution of telomeric heterochromatin in the genus Secale and in triticale. Chromosoma. 1983;88:293–8.
Dille JE, Gustafson JP. Influence of telomeric heterochromatin loss on growth and development of the early embryo and endosperm in triticale. Plant Breed. 1990;104:34–9.
Dundas IS, Frappell DE, Crack DM, Fisher JM. Deletion mapping of a nematode resistance gene on rye chromosome 6R in wheat. Crop Sci. 2001;41:1771–8.
Han FP, Lamb JC, Birchler A. High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proc Natl Acad Sci U S A. 2006;103:3238–43.
Tang ZX, Yang ZJ, Fu SL. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet. 2014;55:313–8.
Yang Z, Zhang T, Lang T, Li G, Chen G, Nevo E. Transcriptome comparative profiling of barley eibi1 mutant reveals pleiotropic effects of HvABCG31 gene on cuticle biogenesis and stress responsive pathways. Int J Mol Sci. 2013;14:20478–91.
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, et al. A fulllength transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotech. 2011;29:644–52.
Mohler V, Stadlmeier M. Dynamic QTL for adult plant resistance to powdery mildew in common wheat (Triticum aestivum L.). J Appl Genet. 2019;60:291–300.
Yuan F, Zeng Q, Wu J, Wang Q, Yang Z, Liang B, Kang Z, Chen X, Han D. QTL mapping and validation of adult plant resistance to stripe rust in Chinese wheat landrace Humai 15. Front Plant Sci. 2018;9:968.
We are thankful to Dr. Ian Dundas at the University of Adelaide, Australia for reviewing the manuscript.
ZY was funded by National Key Research and Development Program of China (2016YFD0102000) and National Natural Science Foundation of China (No. 31971886). The funders had no role in the study design, collection, analysis and interpretation of data, or in the writing of the report or decision to submit the article for publication.
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Li, G., Tang, L., Yin, Y. et al. Molecular dissection of Secale africanum chromosome 6Rafr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust. BMC Plant Biol 20, 134 (2020). https://doi.org/10.1186/s12870-020-02351-1
- Adult plant resistance
- Powdery mildew
- Secale africanum
- Stripe rust