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Fig. 3 | BMC Plant Biology

Fig. 3

From: Molecular dissection of Secale africanum chromosome 6Rafr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust

Fig. 3

FISH of wheat-S. africanum 6Rafr lines among F4 progenies of HH41 × MY11 hybrids. Oligo-pSc119.2 (green) and Oligo-pTa535 (red) probes were used for FISH a to f. Line H536 a contains a pair of 6RafrL telosomes, H297 b has a monosomic iso-6RafrS chromosome, H183 c contains a monosomic iso-6Rafr-2 L chromosome in which 6RafrL lost the two terminal Oligo-pSc119.2 sites, H384 d contains a iso-6RafrL monosome, H316 e contains a 6RafrS telosome, and H320 f contains a deleted 6RafrS telosome. FISH karyotypes g of chromosome 6Rafr and deletion chromosomes are shown after hybridization with probes Oligo-pSc119.2 (left) and Oligo-(CAA)7 (right), respectively. Arrows indicate the S. africanum chromosomes. Bar, 10 μm

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BMC Plant Biology

ISSN: 1471-2229

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