The cotton GhWIN2 gene activates the cuticle biosynthesis pathway and influences the salicylic and jasmonic acid biosynthesis pathways
BMC Plant Biology volume 19, Article number: 379 (2019)
Metabolic pathways are interconnected and yet relatively independent. Genes involved in metabolic modules are required for the modules to run. Study of the relationships between genes and metabolic modules improves the understanding of metabolic pathways in plants. The WIN transcription factor activates the cuticle biosynthesis pathway and promotes cuticle biosynthesis. The relationship between the WIN transcription factor and other metabolic pathways is unknown. Our aim was to determine the relationships between the main genes involved in cuticle biosynthesis and those involved in other metabolic pathways. We did this by cloning a cotton WIN gene, GhWIN2, and studying its influence on other pathways.
As with other WIN genes, GhWIN2 regulated expression of cuticle biosynthesis-related genes, and promoted cuticle formation. Silencing of GhWIN2 resulted in enhanced resistance to Verticillium dahliae, caused by increased content of salicylic acid (SA). Moreover, silencing of GhWIN2 suppressed expression of jasmonic acid (JA) biosynthesis-related genes and content. GhWIN2 positively regulated the fatty acid biosynthesis pathway upstream of the JA biosynthesis pathway. Silencing of GhWIN2 reduced the content of stearic acid, a JA biosynthesis precursor.
GhWIN2 not only regulated the cuticle biosynthesis pathway, but also positively influenced JA biosynthesis and negatively influenced SA biosynthesis.
Plants are constantly stimulated by environmental signals, some of which inhibit growth and development. Plants have developed many structures, such as the cuticle, that increase adaptation or tolerance to these stresses [1, 2]. The plant cuticle is a ubiquitous and chemically heterogeneous lipophilic layer composed of biopolymers, mainly comprising waxes, and cutin, a lipid polymer . The cutin matrix consists mainly of esterified 16/18-carbon hydroxy and epoxy-hydroxy fatty acids (FAs) . The waxes are formed by very-long-chain fatty acid (VLCFA) derivatives , and they cover or are embedded in the cutin matrix. The wax components are produced in three steps. First, 16/18-carbon long-chain acyl-coenzymeAs (C16/C18-acyl-CoAs) are produced from 16/18-carbon long-chain FAs that are catalyzed by long-chain acyl-CoA synthetases (LACSs) in the plastids of epidermal cells . Second, the FAs are extended from C16/C18-acyl-CoAs to VLCFA-acyl-CoAs (>C18, with more than 18 C atoms), catalyzed by fatty acid elongases (FAEs) on the endoplasmic reticulum membrane . FAE enzyme complexes consist of β-ketoacyl-CoA synthase (KCS), β-ketoacyl-CoA reductase (KCR), 3-hydroxyacyl-CoA dehydratase (HCD), and enoyl-CoA reductase (ECR) [8,9,10,11,12] Finally, VLCFA-acyl-CoAs further react to form wax components, mediating the alcohol- or alkane-forming pathways [7, 13,14,15,16]. Many genes encoding enzymes involved in these pathways have been studied.
WIN1 (wax inducer 1) was first reported to transcriptionally activate epidermal wax biosynthesis in Arabidopsis . The barley WIN/SHN gene, Nud, controls grain with adhering hulls by regulating a lipid biosynthesis pathway . The tomato (Solanum lycopersicum) SlWIN3/SHN3 gene regulates cuticle formation in fleshy fruits . WIN transcription factors function redundantly to regulate the epidermal patterning of flower organs in Arabidopsis . Most of the studies about WIN genes have focused on the regulation of cuticle biosynthesis. In addition, overexpression of an Arabidopsis WIN gene in rice activates cellulose biosynthesis and represses lignin biosynthesis . Beyond that, there have been no reports about the influence of WIN on other metabolic pathways.
Jasmonic acid (JA) is an important plant hormone. It is needed for plant growth and development, survival under stress, and throughout the life-cycle. JA is biosynthesized from α-linolenic acid . Linolenic acid is catalyzed by lipoxygenase (LOX) to produce 13-hydroperoxyoctadecatrienoic acid (13-HPOT) . Stearic acid is converted to oleic acid, linoleic acid, and further to α-linolenic acid through a desaturation reaction . Stearic acid has a role into the cuticle biosynthesis pathway . The JA biosynthesis pathway competes with the cuticle biosynthesis pathway for precursors.
Salicylic acid (SA) is an important hormone that is involved in plant immune responses. It regulates the expression of many pathogenesis-related proteins (PRs) . SA is involved in plant defense against Verticillium dahliae [26,27,28,29]. Two pathways of SA biosynthesis in plants have been reported. First, in Arabidopsis, SA appears to be synthesized primarily through an isochorismate-utilizing pathway. Second, phenylalanine forms a substrate in the SA biosynthesis pathway . Both pathways begin with shikimic acid. Lignin is a phenolic heteropolymer that constitutes an important component of plant secondary cell walls, and shikimic acid is the precursor of lignin biosynthsis . The shikimate pathway is responsible for the biosynthesis of tryptophan, tyrosine, and phenylalanine . Phenylalanine is involved in the lignin pathway. AtWIN1 negatively regulates lignin biosynthesis in transgenic rice plants . The biosynthesis of lignin and SA share part of the same pathway . Therefore, WIN has a role in lignin biosynthesis and may also influence SA accumulation.
Ideker  proposed the concept of systems biology in 2001. Briefly, systems biology is the study of living systems not only in terms of separate mechanistic and molecular-level components, but considering many components simultaneously [34, 35]. Metabolic pathways are interconnected, and yet they are relatively independent. For example, various hormone metabolic pathways exert their biological functions synergistically or antagonistically by forming complex and intersecting networks of regulatory pathways . The WIN transcription factor positively regulates the cuticle biosynthesis pathway [17, 37]. Aside from its effects on lignin biosynthesis, it is unknown how the WIN transcription factor influences other metabolic pathways related to the cuticle biosynthesis pathway . Here, we cloned a WIN gene, GhWIN2, from cotton (Gossypium hirsutum), and aimed to determine its role in the regulation of cuticle development and the influence on SA and JA accumulation.
Characterization of GhWIN2
Many WIN/SHN orthologs have been reported to affect various aspects in plant physiological processes [17, 37]. However, less is known about the cotton WIN/SHN orthologs. By using the full-length AtWIN1 amino acid sequence to perform a Blast query against the G. hirsutum genome database (https://cottonfgd.org/), we found eleven sequences of WIN/SHN orthologs in the cotton genome. Those sequences encode six putative amino acid sequences (Additional file 1: Figure S1). Next, primers were designed for full length amplification of those SHN orthologs. Unfortunately, we only cloned Gh_A07G036100 (XP_016720718) from two-week-old cotton seedlings. No expression of other homologous sequences was detected by RT-qPCR in seedings. Phylogenetic analysis revealed that this sequence was closely related to SlSHN2/WIN2 (Fig. 1a) . Thus, we named this gene GhWIN2.
We detected subcellular localization in the transgenic Arabidopsis plants that stably expressed GhWIN2 (Additional file 1: Figure S2 and Figure S3). We selected transgenic Arabidopsis line 4, which had the highest expression levels among the transgenic lines, for analyzing the subcellular localization. DAPI staining was used to stain the nucleus. GhWIN2-GFP and DAPI fluorescence were colocalized in the nucleus (Fig. 1b).
To explore the expression pattern of GhWIN2, we analyzed the GhWIN2 promoter sequence obtained from the G. hirsutum genome database (Table 1). This promoter sequence contains cis-acting elements that are involved in ABA and drought response. The expression of GhWIN2 was strongly induced by abscisic acid (ABA) and sodium chloride (NaCl) treatment in cotton seedlings (Fig. 1c and d).
GhWIN2 activates the cuticle biosynthesis pathway
To examine the functional similarity between GhWIN2 and other known WINs, we detected the expression level of several cuticle-related genes in the wild-type (WT) and transgenic Arabidopsis plants (lines 4 and 10). The selection of those genes was based on previous studies in which the WIN proteins that regulate genes were characterized [17, 38, 39]. The expression of the detected cutin biosynthesis-related genes AtGPAT6 (encoding glycerol-3-phosphate acyltransferase 6), AtGPDHc1 (encoding cytosolic G-3-P dehydrogenase), AtCYP86A4 (encoding cytochrome P450 enzymes), and AtCYP86A7 was greater in lines 4 and 10 than in the WT (Fig. 2a). Expression of the wax biosynthesis-related genes AtKCS1 (encoding 3-ketoacyl-CoA synthase 1), AtCER1 (encoding ECERIFERUM1), and AtCER2 (ECERIFERUM2) was greater in lines 4 and 10, whereas that of AtCER3 (ECERIFERUM3) was less, and that of AtCER6 (ECERIFERUM6) was unchanged, relative to expression in the WT. Expression of the cutin and wax biosynthesis-related gene AtLACS2 (encodes long-chain acyl-coenzyme A synthetase) was greater in lines 4 and 10 than in the WT.
We detected expression levels of the putative cotton orthologs of known Arabidopsis cuticle biosynthesis-related genes in GhWIN2 silenced in cotton plants (hereafter referred to as “TRV:GhWIN2 plants”; Additional file 1: Figure S4 and Figure S5). Expression of the detected cutin biosynthesis-related genes GhGPAT6, GhGPDHc1, GhCYP86A4, and GhCYP86A7 was lower in TRV:GhWIN2 plants than in the TRV:00. Expression of wax biosynthesis-related genes GhCER1 and GhCER6 were lower, whereas that of GhCER3 was unchanged, relative to expression in the TRV:00. Expression of GhLACS2 was also lower in TRV:GhWIN2 plants than in the TRV:00 plants (Fig. 2b). Overall, GhWIN2 up-regulated the expression of the most cuticle biosynthesis-related genes in plants.
Scanning electron microscopy revealed that the transgenic Arabidopsis plants had greater wax crystal accumulation in the stem and a thicker abaxial cuticle layer in the leaf than the WT plants (Fig. 2c, d, and e). These results indicate that GhWIN2 activates the cuticle biosynthesis pathway.
GhCYP86A4 is the target gene of GhWIN2
To explore the role of GhWIN2 in transcriptional activation, we cloned the promoters of GhLACS2, GhCYP86A4, and GhCYP86A7 genes. Using an in vivo transient gene expression assay, we found that only co-infiltration of 35S:GhWIN2 and GhCYP86A4pro-LUC constructs resulted in transcriptional activation (Fig. 3a). Dehydration-responsive element-binding (DREB) and WIN transcriptional factors all belong to the subgroup of AP2/EREBP family that contains an AP2/EREBP domain involved in DNA-binding. Sequence alignment showed that GhWIN2 shares significant sequence identity with other AP2/EREBP proteins at the AP2/EREBP domain (Fig. 3b). From previous studies of DREB proteins, the single amino acid substitution of valine to alanine was sufficient to nullify the interaction between protein and DNA [40,41,42]. The corresponding residues are conserved in WIN proteins (Fig. 3b). Thus, we generated GhWIN2V-A with alanine substitution at the corresponding position. Co-infiltration of 35S:GhWIN2V19A and GhCYP86A4pro-LUC constructs nullified the transcriptional activation (Fig. 3a). In addition, qPCR analysis showed that co-infiltration of 35S:GhWIN2 and GhCYP86A4pro-LUC activated the expression levels of the LUC gene, while co-infiltration of 35S:GhWIN2V19A and GhCYP86A4pro-LUC did not (Fig. 3c and d).
ABA regulates the expression of cuticle biosynthesis genes mediated by GhWIN2
We found that exogenous ABA strongly induced the expression of GhWIN2 (Fig. 1c). To further study the relationship between ABA and GhWIN2, we silenced GhPYL1 and GhNCED1, two key ABA biosynthesis genes in cotton [43, 44], and detected the expression levels of GhWIN2 in TRV:GhPYL1 and TRV:GhNCED1 plants (Additional file 1: Figure S6). Expression of GhWIN2 in TRV:GhPYL1 and TRV:GhNCED1 plants was suppressed after silencing of GhPYL1 and GhNCED1 (Fig. 4a).
To further identify the role of GhWIN2 in ABA-cuticle pathway, expression levels of GhCYP86A4, GhCYP86A7, and GhLACS2 was detected in TRV:00 and TRV:GhWIN2 plants. We found that exogenous ABA induced expression of these three genes in TRV:00 and TRV:GhWIN2 plants, whereas the inducible multiple was lower in TRV:GhWIN2 plants (Fig. 4b-d). These results suggest that ABA induces the expression of cuticle-related genes mediated by GhWIN2.
GhWIN2 negatively regulates plant resistance to fungal pathogens
Previous studies have shown that WIN orthologs positively or negatively regulate plant immune responses, depending on the plant species studied [19, 38]. Here, we were interested in finding out how GhWIN2 functions in cotton immune response. To examine this, we challenged TRV:GhWIN2 and TRV:00 plants with V. dahliae. We detected greater resistance to V. dahliae in TRV:GhWIN2 plants than in the TRV:00 plants (Fig. 5a). An analysis of relative fungal biomass showed that there was less fungus in TRV:GhWIN2 plants (Additional file 1: Figure S7). JA is involved in plant immune response to V. dahliae [45, 46]. Surprisingly, expression of the putative or identified JA biosynthesis-related genes was suppressed and content of JA decreased in TRV:GhWIN2 plants (Fig. 5b and c). However, the content of SA and expression of the SA-responsive genes GhPR1 and GhPR5 was significantly greater in TRV:GhWIN2 plants than in TRV:00 plants (Fig. 5d and e). To determine whether SA enhanced the immune response, we decreased the content of SA by silencing the putative SA biosynthesis gene GhICS2 (TRV:GhICS2) . Challenging with V. dahliae resulted in lower SA content in the TRV:GhICS2 plants and the TRV:GhWIN2/TRV:GhICS2 two-gene-silenced plants than in the TRV:00 and TRV:GhWIN2 plants (Additional file 1: Figure S8). Resistance to V. dahliae was lower in the two-gene-silenced plants than in the TRV:GhWIN2 plants (Fig. 5a; Additional file 1: Fig. S7). These results indicate that SA enhanced resistance to V. dahliae in TRV:GhWIN2 plants.
Next, we detected expression of genes involved in the fatty acid biosynthesis pathway upstream of the cuticle and JA biosynthesis pathway, and assessed the stearic acid content (Fig. 6). We found that expression levels of GhFATA, GhFATB, GhSAD3, GhSAD7, and GhKASII [48,49,50,51] were lower in TRV:GhWIN2 plants than in TRV:00 plants. Content of the stearic acid was also lower in TRV:GhWIN2 plants than in TRV:00 plants.
The aerial parts of land plants are covered by a cuticle, a hydrophobic layer that prevents the epidermal cells from having direct contact with the environment . Many studies about the cuticle have been reported. However, the relationship between the cuticle biosynthesis pathway and other metabolic pathways has rarely been reported. To obtain deeper insight into the cuticle biosynthesis pathway, we cloned GhWIN2 from cotton. We have identified GhWIN2 as a positive regulator of the cuticle synthesis pathway. Our results provide evidence that reduced expression of GhWIN2 negatively regulates JA accumulation and positively regulates SA accumulation, and further confers resistance against V. dahliae.
It is known that WIN transcription factors transcriptionally activate the expression of cuticle synthesis-related genes [13, 52], resulting in cuticle accumulation. In barley, the Nud gene, which is homologous to the Arabidopsis WIN1/SHN1, is responsible for the control of a lipid biosynthesis pathway, generating organ adhesion . We found that GhWIN2 belongs to the WIN protein family; first, it is localized at the nucleus (Fig. 1b); second, its overexpression in Arabidopsis increased the amount of wax crystals on the stem and thickness of abaxial cuticle in the leaf (Fig. 2c, d, and e); third, it transcriptionally regulated the expression of cuticle-related genes (Fig. 2a and b; Fig. 3).
Previous studies have shown that WIN orthologs transcriptionally activate target genes [19, 37, 53]. Here, to explore the role of GhWIN2 in transcriptional activation, we cloned the promoters of GhLACS2, GhCYP86A4, and GhCYP86A7 genes; we selected these genes based on previous studies in which WIN orthologs target genes were identified [19, 37, 53]. We found that GhWIN2 only activates the transcription of GhCYP86A4 promoter (Fig. 3a).
The DREB/ERF-type transcription factor belongs to the AP2/EREBP family . DREB proteins contain two conserved amino acids in the AP2/EREBP domain, of which valine is especially important for DNA-binding [40,41,42]. Consistent with those findings, we found that GhWIN2 is a subfamily in the AP2/EREBP family, and contains a conserved AP2/EREBP domain (Fig. 3b). These analyses point to the potential role of GhWIN2 in transcriptional activation of the corresponding conserved valine. We observed that the single amino acid substitution of valine to alanine was sufficient to nullify the transcriptional activation of GhWIN2 (Fig. 3a).
In Arabidopsis, it has been shown that the expression of many cuticle-related genes is suppressed or induced in ABA biosynthesis mutants, depending on the mutant used and the genes studied . Exogenous ABA inhibits expression of cuticle biosynthesis-related genes in Physcomitrella patens, whereas those orthologs were induced in Arabidopsis . These results led us to detect how ABA regulates expression of cuticle-related genes in cotton. Here, we found that exogenous ABA strongly induced expression of GhWIN2 (Fig. 1c). This is consistent with previous reports that TdWIN1 was strongly induced by ABA in wheat . In addition, expression of GhWIN2 was suppressed in TRV:GhPYL1 and TRV:GhNCED1 plants (Fig. 4a). These results indicate that GhWIN2 is an ABA-responsive transcription factor. In Arabidopsis, the genes MYB16, MYB94, MYB96 and DEWAX are involved in the ABA-cuticle regulatory pathway . There have been no reports that AtWIN1/SHN1 is involved in ABA-cuticle pathway. Here, we found that ABA induced some cuticle biosynthesis genes and that this was mediated, or partially mediated, by GhWIN2 (Fig. 4b-d). Thus, the mechanism of cuticle biosynthesis regulated by ABA is conserved and sophisticated in various species. As previously reported, although ABA generally induces expression of the transcription factors that positive regulate of cuticle biosynthesis, it also suppresses expression of one of these positive regulators, HDG1 .
Previous studies have shown that WIN orthologs positively or negatively regulate immune responses, depending on the plant and pathogen species studied. In Arabidopsis, overexpression of AtWIN1/SHN1 caused reduced expression of PDF1.2, which compromised resistance to Botrytis cinerea and Alternaria brassicicola . However, tomato SlWIN3/SHN3 conferred resistance in fruit against the fungus Colletotrichum coccodes, by causing a thickened cuticle that prevents fungal penetration . Here, we found that GhWIN2 conferred sensitivity to V. dahliae (Fig. 5a). The thickened cuticle may contribute to the increased immune response, but its efficacy against V. dahliae was not great. Expression of PDF1.2 in AtWIN1/SHN1 overexpressed Arabidopsis plants was dramatically lower than in wild-type Arabidopsis plants . In addition, the JA and cuticle biosynthesis pathways share the same precursor, stearic acid [57,58,59,60]. Therefore, reduced expression of GhWIN2 may lead to increased biosynthesis of JA, and this could explain why we the detected expression of JA biosynthesis-related genes (Fig. 7). Expression of putative or identified JA biosynthesis-related genes and content of JA was lower in TRV:GhWIN2 plants than in TRV:00 plants (Fig. 5b and c), probably because that the precursor of JA biosynthesis reduced caused by decreased expression of GhWIN2. Thus, GhWIN2 may regulate the fatty acid biosynthesis pathway upstream of the cuticle biosynthesis pathway and the JA biosynthesis pathway simultaneously. As we predicted, the expression of genes related to stearic acid biosynthesis and stearic acid metabolism was reduced in TRV:GhWIN2 plants (Fig. 6). These results indicated that GhWIN2 regulated not only the cuticle biosynthesis pathway, but also the upstream pathways.
Content of SA and SA-responsive marker genes was substantially lower in TRV:GhWIN2 plants than in TRV:00 plants (Fig. 5d and e). This is consistent with the finding that overexpression of AtWIN1/SHN1 in rice caused a 45% reduction in lignin content . Biosynthesis of SA and lignin share part of the same pathway, and shikimic acid is a precursor to SA and lignin (Fig. 7). Thus, it is likely that WIN regulates biosynthesis of SA and lignin via the same mechanism. This is probably because abnormal expression of GhWIN2 alters metabolic flux redirection. The greater resistance to V. dahliae that we observed in the TRV:GhWIN2 plants was probably caused by the higher SA content in these plants. To evaluate this, we subjected the putative gene GhICS2 involved in SA biosynthesis to functional analysis . VIGS constructs for GhWIN2 and GhICS2 were used together to generate two-gene silenced plants, TRV:GhWIN2/TRV:GhICS2. Content of SA and resistance to V. dahliae was lower in TRV:GhWIN2/TRV:GhICS2 plants than in TRV:GhWIN2 plants. Thus, we conclude that SA conferred resistance to V. dahliae in TRV:GhWIN2 plants.
We obtained eleven sequences of WIN/SHN orthologs in the cotton genome. However, we only cloned GhWIN2 from cotton seedings and did not detect the expression of other sequences, indicating that other orthologs may not be expressed at the seedling stage, or it may be just that we have not detected them. If the latter, silencing of GhWIN2 could have silenced other orthologs simultaneously considering their high homology. Thus, it is worth noting that the observed phenotypes may be the results of silencing of WIN/SHN orthologs.
From the point of view of systems biology, there are intricate connections among different metabolic pathways: they compete for substrates, have different metabolic kinetics, and their products can activate or inhibit other pathways . Studying the connections between different metabolic pathways in plants is important in bioenergy and synthetic biology, and can expand our understanding of whole plant systems. The cuticle is related to plant drought tolerance and disease resistance. It acts as a barrier limiting non-stomatal water loss [4, 61, 62]. Few studies have examined the relationship between the cuticle biosynthesis pathway and other metabolic pathways. Here, we have provided evidence that GhWIN2 not only regulates cuticle biosynthesis pathway, but also positively influences JA biosynthesis and negatively influences SA biosynthesis. The other WIN/SHN orthologs may also be involved in regulation of these physiological processes.
Plant and fungal cultivation
The state cotton variety 2,006,001 (original strain no. GK44) was kindly provided by the Cotton Research Institute, Chinese Academy of Agricultural Sciences. Germination was accelerated before sowing in soil. The cotton seedlings were grown for 2 weeks at day/night temperatures of 26 °C/23 °C in an incubator, using a 16 h light/8 h dark photoperiod cycle.
Arabidopsis thaliana Col-0 wild type (WT) and transgenic Arabidopsis plants were grown in soil in an incubator at 23 °C, 70% relative humidity, with a 16 h light / 8 h dark photoperiod. Seedlings were grown on agar plates containing 1% sucrose and 0.8% agar. Seeds were sterilized before being planted on the plate.
Verticillium dahliae strain Vd991 was cultured on a potato dextrose agar plate for 7 days at 26 °C and then inoculated into Czapek medium for 1 week. The spore suspension (106 spores ml− 1) was then prepared by filtration.
RNA extraction and RT-qPCR
Total RNA was extracted from the transgenic Arabidopsis plants or treated cotton plants using a plant RNA extraction kit (Biomed). Two micrograms of total RNA were reverse transcribed using a Fast Quant cDNA Reverse Kit (Tiangen Biotech Co., Ltd., Beijing, China). RT-qPCR was carried out using a SYBR® Premix Ex Taq (Tli RNaseH Plus) (Takara, Shiga, Japan). The endogenous genes GhUBQ7 (DQ116441) and EF-1-α were used as the control in cotton and Arabidopsis plants, respectively. Reactions were amplified on an ABI7500 thermocycler (Applied Biosystems, Foster City, CA, USA). The transcription levels of GhWIN2 were analyzed by the comparative CT (2-△△CT) method. Relative fungal biomass was detected by RT-qPCR. The V. dahliae specific primers, ITS1-F/ST-Ve1-R, were used (Additional file 1: Table S1).
To obtain GhWIN2-overexpressing Arabidopsis plants, GhWIN2 was amplified using the primers WIN-1300-F/WIN-1300-R (Additional file 1: Table S1), with Pst1 and Spe1 cleavage sites at the 5′ and 3′ ends, respectively. Next, the sequence was inserted into a modified Super-pCAMBIA1300 vector (Additional file 1: Figure S2). The recombinant construct was transformed into Agrobacterium tumefaciens strain GV3101. GV3101 was then used to infect Arabidopsis thaliana to obtain transgenic plants .
Seedlings were grown for five days under standard conditions. To stain nuclei, five day-old transgenic seedlings were submerged in PBS containing 5 μg/mL 4′-6-diamidino-2-phenylindole (DAPI) (Sigma) and then incubated for 15 min before imaging . Imaging of GFP and DAPI was performed with a FLUOVIEW FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan). GFP and DAPI were excited at 405 nm and 488 nm, respectively.
Transient gene expression assay
The promoter sequence of GhCYP86A4 was cloned using primers GhCYP86A4pro-F/GhCYP86A4pro-R (Additional file 1: Table S1). The Gateway cloning system (Invitrogen) was used. The promoter sequence was cloned into vector pGWB435, which contains an LUC reporter gene . The construct GhCYP86A4pro:LUC was then transformed into Agrobacterium tumefaciens strain GV3101. The GV3101 was cultured in LB medium containing 50 mg/mL spectinomycin and 50 mg/mL rifampicin. The coding sequence of GhWIN2 and GhWIN2V-A was cloned into the vector pROK2 to generate 35S:GhWIN2 and 35S:GhWIN2V-A. These two constructs were then transformed into Agrobacterium tumefaciens strain GV3101. GV3101 harboring 35S:GhWIN2 or 35S:GhWIN2V-A was then cultured in LB medium containing 50 mg/mL kanamycin and 50 mg/mL rifampicin. For transient expression assay in tobacco, the Agrobacterium tumefaciens strain GV3101 cells containing GhCYP86A4pro:LUC and 35S:GhWIN2/35S:GhWIN2V-A were mixed and treated with infiltration buffer (200 mM acetosyringone, 10 mM MES, pH 5.6; 10 mM MgCl2) for 3 h. Next, the mixed cultures were injected into the leaf using a needleless syringe. The plants were then grown in the dark for 24 h and then under normal growth conditions for 48 h. The LUC signal was measured using a CCD camera (1300B; Roper) after being sprayed with 1 mM luciferin (Sigma-Aldrich).
Virus-induced gene silencing
The fragments of GhWIN2 was amplified and cloned into an improved pTRV2 virus-induced gene silencing (VIGS) vector, pYL192 [65,66,67]. The recombinant plasmid pTRV2:GhWIN2 was then transformed into Agrobacterium tumefaciens strain GV3101. Gene silencing was conducted according to the method described in Li et al. . Agrobacterium cultures harboring pTRV1, pTRV2, or pTRV2:GhWIN2 were grown in LB medium containing 50 μg/mL rifampicin, 50 μg/mL kanamycin, 20 μM acetosyringone, and 10 mM MES until the OD600 reached a value of 1. The cotyledons of 14-day-old cotton seedlings were injected with a mixture of the Agrobacterium cultures harboring the pTRV1/pTRV2 plasmids (1:1 ratio, v/v) as the control and pTRV1/pTRV2:GhWIN2 plasmids (1:1 ratio, v/v) as the experimental group. We also constructed pTRV2:GhCLA1 (Cloroplastos alterados 1; 500 bp) to detect the efficiency of silencing under our experimental conditions.
AtGPAT6, AT2G38110; AtGPDHc1, AT2G41540; AtCYP86A4, AT1G01600; AtCYP86A7, AT1G63710; AtKCS1, AT1G01120; AtCER1, At1G02205; AtCER2, At4G24510; AtCER3, At5g57800; AtCER6, At1g68530; AtLACS2, At1G49430; GhGPAT6, ADK23938.1; GhGPDHc1, XP_016671566.1; GhCYP86A4, XM_016840837.1; GhCYP86A7, XP_016719401.1; GhCER1, XP_016681695.1; GhCER3, XP_016725538.1; GhCER6, KT625616.1; GhLACS2, XP_016707966.1; GhAOC5, KF383427.1; GhAOS, ALG62633.1; GhLOX1, AF361893.4; GhOPR3, NP_001313917.1; GhFATA, XP_016727762.1; GhFATB, XP_016720478.1; GhSAD3, XM_016885870.1; GhSAD7, XM_016843547.1; GhKASII, HM236494.1; GhPYL1, XM_016815548.1; GhNCED1, HM014161.
Availability of data and materials
The data sets during the current study are available from the corresponding author.
16/18-carbon long-chain acyl-coenzyme A
Cytochrome P450 enzymes
Fatty acid elongases
Glycerol-3-phosphate acyltransferase 6
Cytosolic G-3-P dehydrogenase
3-ketoacyl-CoA synthase 1
Long-chain acyl-coenzyme A synthetase
Long-chain acyl-CoA synthetases
Virus-induced gene silencing
Very-long-chain fatty acid
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We thank Prof. Yule Liu (Tsinghua University) for the VIGS vectors and Prof. Jinsong Zhang (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for the vector pGWB435.
This work was sponsored by the ‘Seven Crop Breeding’ National Major Project (grant no. 2016YFD0101006); the National key research and development program “chemical fertilizers and pesticide reduction efficiency integrated technology research and development” (grant no. 2017YFD0201900); the State Key Laboratory of Cotton Biology (grant no. CB2019B04). These funding bodies had no influence on the design of the study, collection, analysis, interpretation of data, or the manuscript writing.
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Supplementary information. Figure S1 Amino acid sequence alignment of cotton WIN orthologs. Figure S2 Schematic representation of the plant expression vector pCAMBIA1300-GhWIN2. Figure S3 Relative expression of GhWIN2 in transgenic Arabidopsis plants. Figure S4 Expression of GhWIN2 in control and silenced cottons. Figure S5 VIGS-mediated silencing of GhCLA1 in cotton. Figure S6 Relative expression of GhPYL1 and GhNCED1 in cotton plants 14 days after agroinfiltration. Figure S7 Relative biomass of V. dahliae in infected cotton plants. Figure S8 VIGS-mediated silencing of GhICS2 in cotton. Table S1 Primers used in this study. (PDF 1037 kb)
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Li, X., Liu, N., Sun, Y. et al. The cotton GhWIN2 gene activates the cuticle biosynthesis pathway and influences the salicylic and jasmonic acid biosynthesis pathways. BMC Plant Biol 19, 379 (2019). https://doi.org/10.1186/s12870-019-1888-6