- Research article
- Open Access
Transcriptome characterization of moso bamboo (Phyllostachys edulis) seedlings in response to exogenous gibberellin applications
BMC Plant Biology volume 18, Article number: 125 (2018)
Moso bamboo (Phyllostachys edulis) is a well-known bamboo species of high economic value in the textile industry due to its rapid growth. Phytohormones, which are master regulators of growth and development, serve as important endogenous signals. However, the mechanisms through which phytohormones regulate growth in moso bamboo remain unknown to date.
Here, we reported that exogenous gibberellins (GA) applications resulted in a significantly increased internode length and lignin condensation. Transcriptome sequencing revealed that photosynthesis-related genes were enriched in the GA-repressed gene class, which was consistent with the decrease in leaf chlorophyll concentrations and the lower rate of photosynthesis following GA treatment. Exogenous GA applications on seedlings are relatively easy to perform, thus we used 4-week-old whole seedlings of bamboo for GA- treatment followed by high throughput sequencing. In this study, we identified 932 cis-nature antisense transcripts (cis-NATs), and 22,196 alternative splicing (AS) events in total. Among them, 42 cis-nature antisense transcripts (cis-NATs) and 442 AS events were differentially expressed upon exposure to exogenous GA3, suggesting that post-transcriptional regulation might be also involved in the GA3 response. Targets of differential expression of cis-NATs included genes involved in hormone receptor, photosynthesis and cell wall biogenesis. For example, LAC4 and its corresponding cis-NATs were GA3-induced, and may be involved in the accumulation of lignin, thus affecting cell wall composition.
This study provides novel insights illustrating how GA alters post-transcriptional regulation and will shed light on the underlying mechanism of growth modulated by GA in moso bamboo.
Gibberellins (GA), are one of the most important class of growth-promoting phytohormones, play crucial roles in many aspects of plant growth and development, especially in growth promotion and flower induction [1,2,3]. GA regulate growth by suppressing a group of DELLA nuclear repressor proteins , and display extensive cross-talk with other hormone signaling pathways, including auxin and brassinosteroids (BR) [4,5,6]. Moso bamboo, native to China and Taiwan, is now grown worldwide for commercial purposes thanks to its very fast growth [7, 8]. Transcriptome analysis in the shoots of moso bamboo revealed hormone-related genes are involved in both growth directions, that is in height and in thickness [9, 10]. A later proteomics study showed seven protein spots involved in hormone biosynthesis during shoot development . The concentration of endogenous GA3, one of most common bioactive forms of gibberellic acid , displays bimodal variation when the above-ground heights grow from 0.05 m to 12 m in moso bamboo [11, 13, 14]. The extensibility of moso bamboo cell walls is a key factor in moso bamboo’s growth, as they allow grow to great heights due to the two primary components of bamboo walls, cellulose and lignin . Lignin, a main resource for biofuels, functions as a transport vessel to maintain the structural and mechanical integrity of the entire plant . Cellulose fibrils maximize longitudinal elasticity while lignification primarily increases the transversal rigidity of the fibrils , which overall provides structural support. Proteins involved in cell wall biosynthesis are reported to be related to rapidly elongating bamboo culms . Although endogenous hormone studies have been conducted in the shoots of moso bamboo , the direct link between hormone activity and post-transcriptional regulation remains unknown. In addition to post-transcriptional regulation by alternative splicing (AS), natural antisense transcripts (NATs) also play important roles. NATs take part in post-transcriptional regulation by partial or complete complementation of other transcripts [19,20,21]. Cis-NATs predominantly regulate the same genomic loci with perfect complementarity, which can be categorized into three types: head-to-head, tail-to-tail, and fully overlapping . Genome-wide identification of NATs in mouse, human, rice, and Arabidopsis thaliana revealed that natural antisense transcription is a widespread phenomenon [23,24,25,26,27,28,29]. In this study, we provide the genome-wide analysis of cis-NATs in moso bamboo using PacBio single-molecule real-time sequencing (SMRT) . In combination with RNA-Seq in this study, we revealed differential cis-NATs and AS events upon GA induction, which provides novel insight into GA-mediated post-transcriptional regulation in moso bamboo.
Exogenous gibberellin applications result in increased internode elongation in moso bamboo
To determine whether GA is involved in bamboo growth, 2-week-old bamboo seedlings were treated daily with GA3 or the paclobutrazol (PAC, a specific inhibitor of GA biosynthesis inhibitor) for two weeks, and the effects on moso bamboo’s growth were then observed (Fig. 1a). Internode and stem lengths significantly increased in moso bamboo treated with exogenous GA3 applications. In contrast, PAC significantly retarded stem elongation of moso bamboo as previously reported from many other species [31,32,33], and exogenous GA3 could reverse the inhibition of PAC (Fig. 1b). Leaf width was also sensitive to GA3, whereas root length was not altered by GA3 applications. The results showed that GA3 promoted stem growth of bamboo mainly by affecting internode elongation.
Transcriptome-scale analysis of GA-responsive genes
To better understand the underlying molecular mechanism, we used RNA-Seq to profile the transcriptomes of GA-treated and non-treated moso bamboo. In total, we obtained 130,988,922 and 153,580,552 reads with three biological repeats from GA-treated and non-treated control libraries, respectively. The mapping rate for each library was above 88%. We obtained 47,927,346 and 56,985,350 uniquely mapped reads for further analysis (Additional file 1: Table S2). By comparative analysis, we identified 5148 significantly differentially expressed genes (DEGs), of which 3025 were GA-induced and 2123 were GA-repressed (Fig. 2a, Additional file 2: Table S3). To verify the results from RNA-Seq, 14 genes were selected for further confirmation by qRT-PCR, and 10 of these genes showed expression profiles consistent with the RNA-Seq results, including 6 GA-induced and 4 GA-repressed genes, indicating that the RNA-Seq data was reliable (Fig. 2b,Additional file 3: Table S1). Gene Ontology (GO) analysis showed that these DEGs are involved in photosynthesis, lignin metabolic process, response to chemical stimulus and others (Fig. 2c).
GA response and biosynthetic genes in response to exogenous GA
From GO enrichment analysis, 61 up-regulated DEGs were assigned to the functions of “hormone-mediated signaling pathway” and “cellular response to hormone stimulus” (Additional file 4: Table S4). In total, 28 genes involved in “GA biosynthetic process” and “responding to GA” significantly changed in response to the application of exogenous GA (Fig. 3a). For example, the expression of GA20OX1, a GA-biosynthesis gene , was reduced upon exogenous GA treatment in moso bamboo. Additionally, the GA receptors GID1A and GID1B were down-regulated, and the GA repressor genes SLR1, SLN1, and GAI were up-regulated. Since the concentration of exogenous GA was saturated in our study, the observed repression of GA biosynthesis and signaling genes may be due to the negative feedback regulation of endogenous GA biosynthesis in response to the high concentrations of exogenous GA (Fig. 3a).
Other hormone-related signaling pathways upon exogenous GA applications
Interestingly, genes associated with other hormone signaling pathways were also significantly overrepresented upon GA3 treatment (Fig. 3b, Additional file 4: Table S4). For example, GO terms associated with responses to auxin were also enriched. These results support the idea that the GA signaling pathway crosstalks with the auxin signaling pathway, which is consistent with other previously well-studied plant species . In moso bamboo, 883 hormone-related genes were identified from the search for homologs of the Arabidopsis horone database . Among them, 188 were significantly differentially expressed, and they were mainly involved in the eight principal classes of plant hormones: abscisic acid (ABA), auxin, BR, cytokinins (CK), ethylene, GA, jasmonates (JA) and salicylic acid (SA) (Fig. 3c, Additional file 5: Table S5). This study indicates that, at least in moso bamboo, exogenous GA applications can affect other hormone-related pathways, which is consistent with previous studies [36, 37].
Exogenous GA treatment influences photosynthesis
Among the identified GA-repressed genes, functions related to photosynthesis were most enriched (Fig. 4a, Additional file 4: Table S4), prompting us to hypothesize that GA treatment may affect photosynthesis in GA3-treated bamboo. To test this, leaf chlorophyll content was measured in GA3, H2O, both GA3 and PAC, and PAC treated seedlings (Fig. 4b). The results showed that the chlorophyll content was increased in the PAC treated seedlings, while reduced in exogenous GA3 treatments, providing additional evidence that GA3 can affect photosynthetic capacity.
To further strengthen the connection between GA and photosynthesis, the leaf photosynthetic rate was also measured, and the results showed that exogenous GA3 treatment reduced the photosynthetic rate in comparison with H2O-treated seedlings (Fig. 4c). PAC reduced the photosynthetic rate, and subsequent exogenous GA3 treatment partially reversed the PAC-induced repression. These results suggested that exogenous GA treatment may affect the regulation of photosynthesis to adjust the carbon cycle to the development of seedlings.
Genes involved in the formation of cell walls
Based on transverse sections of the first elongated internode of stems, more intense staining was found in the vessels and fibers after the application of bioactive GA3, thus indicating increased lignification. In contrast, PAC treatment dramatically reduced lignification (Fig. 5a). In addition, exogenous GA3-treatment rescued the effects of PAC. These histological observations were further confirmed by the lignin content determination (Fig. 5b). Together, these results showed that GA treatment significantly increased the lignification of stems (P < 0.001), similarly to findings with winter wheat, dwarf pea and tobacco [38,39,40,41].
Given that the cell wall architecture is a key determinant for plant growth, we focused on 66 cell-wall-related genes, which are clustered into three groups: cellulose synthase genes (Ces A), cellulose synthase-like genes (Csl) and lignin biosynthesis related genes . Among them, we identified 12 DEGs. Two members of CesA gene family, CESA6 and CESA7, were dramatically up-regulated upon GA treatment (Fig. 5c). CSLF8, CSLG1, CSLA1 and CSLF2 were repressed in response to GA applications, while CSLE1 was GA-induced (Fig. 5c). Cinnamoyl-CoA reductase (CCR) catalyzes the first step in the monolignol biosynthetic pathway, and a higher concentration of CCR protein corresponds to increase in lignification [42, 43]. In moso bamboo, GA-induced CCR gene suggested that they might take part in the regulation of lignification biogenesis. Considering the significant up-regulation of CCR, we propose that the accumulation of lignin after GA3 treatment may result from the transcriptional activation of lignin biosynthesis related genes.
GA affects the AS
During shoot growth, 60% of genes display alternatively spliced isoforms in moso bamboo . According to cufflink assembler, we identified 233,495 unique coordinates for introns, which represented splice-junction regions. Among these splicing junction sites, we identified 22,196 AS events in seedlings using RNA-Seq. Among them, there were 442 differential AS events classed into four distinct types: 113 intron retention (IR), 120 exon skipping (ES), 135 alternative acceptor (AltA), 74 alternative donor (AltD) (Additional file 6: Table S6). For example, RCA (PH01003146G0230), which is involved in the response to light stimulus , was found to generate different isoforms by AltD. RT-PCR and qRT-PCR with isoform-specific primers further validated several selected AS events, such as PH01000276G0360, ATAD3, PAE9, RAB6A and NOT2A, which further suggested that RNA-Seq was reliable (Fig. 6, Additional file 3: Table S1). Longer isoforms of ATAD3, PAE9 and RAB6A were up-regulated, and NOT2A was down-regulated upon exogenous GA3 treatment (Fig. 6). ATAD3, a mitochondrial membrane ATPase, is highly conserved from human to plants [46, 47]. ATAD3 generated two isoforms with an alternative exon skipping, and GA treatment repressed the exon skipping and generated longer isoform (Fig. 6b). The additional exon encoded a potential 39 aa in the coiled-coil region by a homology search in C.elegans (UniProt, Q20748). Further experiments are needed to study whether exon skipping of ATAD3 affects its subcellular localization or function.
In total, 19 genes with GA-responsive splice isoforms, were related to hormone signaling pathway (Additional file 7: Table S7). For example, PIF1 (IR, PH01001389G0560) is involved in GA signaling pathway, which directly regulates DELLA (GAI and RGA) (Additional file 7: Table S7) . Additionally, GRF1 (IR, PH01001291G0040) takes part in the gibberellin signaling pathway; it is reported to associate with stem elongation in rice  and UV-B repression in maize . In addition, we identified 9 genes encoding splicing factors, generating different isoforms after GA treatment (Additional file 7: Table S7). It is important to note that two splicing factor genes, RNP_N1 (PH01000290G0450:ES), RSP40/RSP35 (PH01000347G1190:ES) are also hormone-related , and have differentially expressed AS events. Overall, these results strongly suggest that AS accommodates to GA3 action in moso bamboo.
GA affects the NATs
Cis-NATs regulate transcripts from the same genomic loci with perfect complementarity. Using PacBio full-length transcripts, we revealed genome-wide NATs and identified 932 cis-NATs classed into three types: head-to-head (5′-5′), tail-to-tail (3′-3′) and fully overlapping (Additional file 8: Table S8). Genome-wide analyses showed that most NATs are generated from long noncoding RNA (lncRNA) in both mammals  and plants . Among the 932 cis-NATs in moso bamboo, there were 492 pairs of cis-NATs including long noncoding RNAs, which represented long noncoding natural antisense transcripts (lncNATs). In total, there were 42 differentially expressed cis-NATs (Additional file 9: Table S9). We selected 6 pairs of cis-NATs for further validation by RT-PCR, and all of them could be amplified with the expected band (Fig. 7), demonstrating the advantage of the identification of NATs using PacBio full-length transcripts. Among them, the changes of 4 pairs were apparently identical to in silico analysis, while the other two were not obvious by RT-PCR. It is possible that this discrepancy is due to the limited sensitivity of agarose gel electrophoresis, so real-time PCR was carried out. qRT-PCR results showed expression profiles consistent with RNA-Seq results except for one pair of cis-NATs (Fig. 7), which suggested the ability of GA to regulate NATs.
In total, there are 3 and 21 cis-NATs targeted genes involved in “hormone metabolic process” and “response to endogenous stimulus”, respectively. Four genes are part of “cell wall biogenesis”, and 43 genes were assigned to the function of “oxidation reduction” (Additional file 10: Table S10). In this study, we revealed that ABAR (PH01000056G0440) and AMY1 (PH01001272G0030) in moso bamboo were regulated by cis-NAT PH01000056G0430 and PH01001272G0020, respectively (Fig. 8), and it has been reported that ABAR and AMY1 are associated with hormone receptor  and gibberellin . Our data showed that the kinase gene STN8 (PH01003235G0120) is involved in phosphorylation of photosystem II core protein, and could be targeted by cis-NATs, which are located in the intron of STN8. The transcript abundance of STN8 was dramatically down-regulated upon GA-induced growth (Fig. 8). These results indicated that cis-NATs may participate in reducing photosynthetic capacity in response to GA3 treatment. LAC4 is involved in constitutive lignification of stems together with LAC17 . It is important to point out that in bamboo, GA-induced LAC4 (PH01001798G0410) and its corresponding cis-NATs are both GA-inducible and affect cell wall biogenesis (Fig. 7a). The data shows that cis-NATs may be another way to regulate moso bamboo’s growth upon GA treatment.
Transcript and post-transcriptional regulation of TFs
A previous study showed that GA signaling regulates the activity and protein stability for TFs of PIF3 and HY5 . HY5 acts as a convergence point of light and multiple hormone signaling pathways [58,59,60]. According to the homologs search, we identified 678 TFs. Among them, 132 TFs are DEGs, including HY5 (PH01000075G0960), a bZIP transcription factor (Fig. 8 and Additional file 11: Table S11). TFs affect AS by influencing the transcription elongation rate of RNA polymerase II . Reciprocally, AS also affects the splicing of TFs, and 13 out of 678 TFs have differential AS events (Additional file 12: Table S12). For example, PIF1 (PH01001389G0560), an important hub gene in both GA and light signaling pathway , and it is regulated by IR (Fig. 8). In addition, ILR3 (PH01000773G0410) and BIM1 (PH01004969G0010) have complementary loci to cis-NATs, which imply that TFs may also be under the regulation of NATs (Fig. 8), though more evidence is required to elucidate this.
Gibberellin affects photosynthesis
Earlier published studies revealed a contradictory relationship between GA and photosynthesis [62, 63]. Some studies reported a positive effect of GA on photosynthesis [64, 65], while others showed a decreased rate of photosynthesis after exogenous GA applications , and one study reported no difference . This study uncovered that exogenous GA3 significantly reduced chlorophyll content, in agreement with the decreased photosynthetic rate, which was similar to the findings with the the slow-growing inbred lines of Plantago major L. . A study in Populus showed that the same concentration of GA3 plays a positive role in regulating photosynthesis . The difference may be due to the different conditions of the treatment or different developmental stages. This study used GA3 to treat two-week-old seedlings daily for two weeks. However, the study in Populus used one year old plants with weekly GA treatments for one month . At present, the effect of PAC on photosynthetic efficiency is also not clear. It is reported that PAC has different effects, including no effect on photosynthetic rate in ‘delicious’ apples , negative effect in wheat and sweet orange [70, 71], and positive effect in radish and Catharanthus roseus [32, 72]. In this study, PAC reduced the photosynthetic net rate slightly, which may be due to the PAC concentration being slightly high and resulting in a phytotoxia reaction. A series of treatments of different concentration of GA with a time-course should be carried out to elucidate the exact relationship between GA, PCA and photosynthesis.
Gibberellin affects lignification
In this study, we found that GA3 treatment increased lignification, which was consistent with previous studies in other plant species [38,39,40,41]. At present, we only investigated the short-term effects of GA3 treatments on lignification. In the future, it will be interesting to observe the long-term effects of GA3 on the biosynthetic pathway of lignification, which has been reported to differ from the short-term effects .
The higher expression of LAC4 results in accumulation of lignin, which is also observed in C.sinensis . As mentioned above, LAC4 is a GA-induced lignification related gene, which was found to be regulated by cis-NATs in moso bamboo. It is also reported to be regulated by transcription factors, for example MYB58 and MYB63 . In moso bamboo, MYB58 (PH01000386G0660) was GA-repressed and MYB63 (PH01000030G0050) was GA induced. In addition, microRNAs may also be involved in regulating the expression of LAC4. For example, miR397 is predicted to target the fifth exon of LAC4 and act as a negative regulator of laccases transcripts . Recent studies show that miR397a and miR397b regulate lignin content by regulating the laccase genes, for example LAC4 [73, 76]. Thus, we propose that up-regulation of LAC4 in moso bamboo results from the action of multiple players, such as TFs, cis-NATs and even microRNA, suggesting the existence of diversity in the regulation network of lignin upon GA treatment.
Gibberellin affects AS
Though previous studies showed that HAB1 generates two spliced isoforms, HAB1.1 and HAB1.2, which function as opposite regulators in ABA sensitivity , there is a lack of genome-wide analysis of AS upon hormone treatment. The comparison of AS changes between mock and GA treatment samples allowed us to identify several hundreds of differential AS events. In this study, we identified 442 differential AS events. Experimental validation showed that qRT-PCR with isoform-specific primers was more sensitive than RT-PCR to quantify the expression of the isoforms. In this study, the longer isoform of ATAD3 was GA induced (Fig. 6b). Searches of public database revealed that ATAD3 is relatively conserved in Arabidopsis, Oryza sativa and Brachypodium distachyon, but is not yet well studied in other plants. Further bioinformatics analysis showed that the skipped exon encoded 39 aa within the coiled-coil region, which suggested that exon skipping functions in the interaction with partners . More experiments are needed to study whether the GA-affected exon skipping of ATAD3 affects its subcellular localization or functions. ATAD3 may be a good case study to investigate the links between the different isoforms and distinct function, and will provide a better understanding of how GA-induced AS affects the growth and development of moso bamboo.
Gibberellin affects NATs
In this study, we took advantage of the full-length transcripts from PacBio sequencing to profile 932 cis-NATs, which avoids likely error-containing transcriptome assembly. GA regulates growth throughout the plant life cycle. Thus, the number of cis-NATs is underestimated and more differential cis-NATs will be revealed using more time points or tissues. In this study we investigated the expression of 932 cis-NATs using strand-specific library, and revealed part differential cis-NATs with experimental validation using both RT-PCR and qRT-PCR. A well-defined natural antisense gene pair is P5CDH and SRO5, which overlaps with each other by the tail-to-tail model and generates endogenous nat-siRNA related with salt stress . Our data revealed that many GA-responsive genes in moso bamboo, such as ABAR, AMY1, STN8 and LAC4, may be regulated by cis-NAT. However, there is no adequate experimental evidence to show whether they generate differential nat-siRNAs. In the future, the nat-siRNA from the complementary regions of overlapping transcripts can be investigated using small RNA sequencing methods, which is optimized for the identification of the potential mechanism of cis-NATs. Except for nat-siRNA, the expression of antisense RNA induces the silencing and methylation of corresponding genes . It will be interesting to reveal the role of NAT-mediated DNA methylation in response to GA in the future.
FLC (FLOWERING LOCUS C) acts as a repressor of flowering, which is a well-characterized example for lncNATs. The antisense transcripts of FLC are induced by cold and the non-coding transcripts act to silence FLC transcription transiently . In this study, we found that more than 50% pairs of cis-NATs included lncNATs. However, the mechanism of lncNATs remains unknown in moso bamboo. At present, a method of regeneration and transformation is still lacking in moso bamboo. Overexpressing antisense transcripts in protoplasts might reveal stage-dependent regulation of lncNATs in the corresponding sense transcription.
In order to reveal how GA regulates growth in moso bamboo, we applied exogenous gibberellins (GA3) on moso bamboo seedlings, and observed that it could significantly increase internode length. Determination of chlorophyll content and photosynthetic rate revealed that GA had a negative effect on the photosynthetic activity in moso bamboo. In addition, lignin visualization with free-hand sections showed the increased accumulation of lignin in response to GA treatment, implying a functional role of GA in bamboo growth by affecting cell wall composition. Taking full advantage of the PacBio single-molecule real-time sequencing (SMRT), we provided the first genome-wide analysis of cis-NATs in moso bamboo. In combination with strand-specific RNA-Seq, we revealed differential cis-NATs and AS events upon GA induction, which further suggested that post-transcriptional regulation is also involved in the GA response in moso bamboo. This study provides novel insights into GA-mediated post-transcriptional regulation on growth and development in moso bamboo.
For RNA sequencing, three biological replicates of 4-week-old whole seedlings grown under long-day conditions (16 h light/8 h dark) were collected from MS (Murashige and Skoog) medium after being treated with H2O or GA3 (100 μM) for 4 h. The plant materials were snap frozen in liquid nitrogen and stored at − 80 °C for total RNA extraction and library construction. Total RNA was isolated with the RNAprep Pure Plant Kit (Tiangen, Cat. #DP441, China) following the manufacturer’s protocol. RNA quality was assessed using an agarose gel and NanoDrop 2000c UV-Vis Spectrophotometer (ThermoFisher, USA). The integrity of RNA samples was further evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Two-week-old bamboo seedlings were sprayed with H2O or GA3 (100 μM), PAC (200 μM) seven times a week for two weeks, and their phenotypic characteristics were recorded. Chlorophyll content, photosynthetic rate and lignin content were analyzed with the above treatment material.
Determination of chlorophyll and photosynthetic rate
To determine the chlorophyll content, ~ 0.1 g fresh leaf material was cut into pieces and added to 10 ml of an ethanol, acetone and distilled water (4.5:4.5:1, v/v/v) mixture to extract chlorophyll. Then the 15 ml centrifugation tube was wrapped in aluminium foil and placed in the dark for three days, until the sample turned white. Finally, 3 ml of each chlorophyll solution were taken from the tube and added to a 10 mm cuvette to measure optical density at 645 nm and 663 nm with Thermo Fisher Multiskan™ FC microplate photometer. The experiments were performed with at least 6 replicates and chlorophyll content was then calculated using Arnon’s equations :
Net photosynthetic rate of bamboo seedlings was calculated with the LI-6400XT Portable Photosynthesis System (LI-COR Corporate, USA) under normal growing conditions. More than 30 first fully expanded leaves of distinct seedlings were used for each treatment. Each leaf was measured with five repeats to obtain an average value. These leaves were marked sequentially and scanned with a Microtek ScanMaker i800 plus system (WSeen, Hangzhou, China). ImageJ was used to calculate the area of leaves accurately. Finally, the photosynthetic rate was calculated using the formula (CO2R-CO2S*(1000-H2OR)/(1000-H2OS))*fda, where CO2R and CO2S was the CO2 concentration from reference and sample cell, respectively. H2OR and H2OS was H2O concentration from reference and sample cell, respectively. Fda was referred Flow/Area of leaf.
Lignin visualization with hand-cut sections and lignin content measurement
For lignin visualization, free-hand sections of 4-week-old seedlings’ first internode were obtained. Then above material was stained with phloroglucinol-HCl (1% (weight/volume) phloroglucinol in 6 mol/l HCl) for 5 min and then observed under a light microscope.
Lignin of the first elongated internode of stems was quantified by modifying the acetyl bromide spectrophotometric method . Samples were ground into powder, 95% ethanol was added immediately and centrifuged for 5 min at 2600 g. The above material was then washed twice with 95% ethanol, after which the pellet was rinsed twice with ethanol-hexane (1:2, v:v). After air drying the samples, the pellets were dissolved with 25% bromine acetyl acetic solution, and then incubated at 70 °C for 30 min. The staining reaction was stopped with 0.18 mL NaOH, 0.02 mL 7.5 mol/L hydroxylamine hydrochloride, and glacial acetic acid was added to reach a final volume of 3 ml. The supernatant was collected after centrifugation at 1000 g for 7 min, and the lignin content was estimated reading the absorbance values at 280 nm.
Library construction for RNA sequencing
RNA-Seq libraries for H2O and GA3 treated samples were constructed by the dUTP method according to the manufacturer’s instructions (Illumina, San Diego, CA, USA), as described in our previous study . Paired-end reads (read length = 125 bp*2) were sequenced with Illumina HiSeq™ 2500 (Illumina, San Diego, CA, USA). All the RNA-Seq raw data has been uploaded to NCBI under accession number GSE104596.
Differential expression analysis
TopHat2 was selected as the mapping tool to map these reads to the moso bamboo reference genome [7, 83] with anchor length more than 8-nt for spliced alignments. Only uniquely mapped reads were retained for subsequent analysis. The bigwig files have been uploaded to our website: http://www.forestrylab.org/pub/GA. The normalized expression levels for gene models were measured as fragments per kilobase of transcript per million mapped reads (FPKM) . False discovery rates (FDR) were calculated using edgeR package . Differential gene expression was identified using fold change > 1.5 and FDR < 0.01.
Identification of differential AS events
The genome sequence of moso bamboo is available. Thus the identification of alternative splicing is similar to the reference-based method [86, 87]. All 125 bp mapping RNA-Seq paired-end reads were assembled using Cufflinks v2.1.1  with the following option: -F 0.05 -A 0.01 -I 100000 --min-intron-length 30 to generate all potential transcripts including all the exons in this study. Then all above isoforms were combined into one annotation of transcripts in GTF format using the Cuffmerge utility within the Cufflinks v2.1.1 package with the following option: --min-isoform-fraction 0.01. The above assembled isoforms and the mapping reads from for H2O and GA3 treated samples were loaded into rMATS.3.2.2  to identify differential AS events using the following parameters: -t paired -len 125 -a 8 -c 0.0001 -analysis U. rMATs output includes all the AS events, including intron retention, exon skipping, alternative acceptor, and alternative donor. In total, AS events with P < 0.05 were regarded as the differential AS events.
GO enrichment analysis
Blast2GO optimizes BLAST algorithm to identify homologous sequences and then assigns uncharacterized novel sequences in non-model species with functional annotation, which is represented via the GO vocabulary . In this study, GO terms of each gene in moso bamboo were assigned using BLAST2GO with default option , and the enrichment of GO terms was assessed with the BiNGO plugin  for Cytoscape , using hypergeometric test for statistical analysis. For P-value correction, we selected the FDR correction method. GO terms with corrected P ≤ 0.05 were considered significantly over-represented and shown as colored nodes in the enrichment network.
Identification of NATs using PacBio SMRT
PacBio SMRT dataset from our previous study  was used for the identification of NATs by our reference-based method . Firstly, the full-length transcripts from PacBio SMRT sequencing with overlapping coordinates from the reference genome were clustered together into transcripts units. Then three types of NATs (head-to-head, tail-to-tail, and fully overlapping) were identified by pair-wise comparison of oppositely oriented full-length transcripts according to overlapping coordinates. Read counts from each NATs were calculated using strand-specific RNA-Seq libraries. The edgeR  was used to calculate FDR. Differential NATs were identified using FDR less than 0.01. The lncNATs was predicted using Coding-Non-Coding Index (CNCI) with default option .
Searching for hormone-related and transcript factors
The InParanoid algorithm uses NCBI-Blast as the homology detection tool to calculate pairwise similarity scores . Thus, we searched the homologs in Arabidopsis to acquire all the homologous genes in moso bamboo by InParanoid (version 3.0) using the default options . In total, we obtained 1,4139 pairwise homologs. All the hormone-related genes and transcript factors (TFs) in Arabidopsis were downloaded from http://ahd.cbi.pku.edu.cn  and Steve A. Kay’s study, respectively .
Experimental validation using quantitative real-time PCR
Primers were designed using premier 5 (Premier Biosoft International, Palo Alto, CA, USA) and synthesized at BGI-Tech (Shenzhen, China). All the information for each primer pair can be found in the Additional file 3: Table S1. RNA-Seq results, including differential expression and NATs were validated by qPCR with SYBR® Premix Ex Taq ™ II (TaKaRa, Cat. # RR820A, Japan) on Applied Biosystems QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher, USA). For each experiment, NTB or UBQ was analyzed in triplicates as the positive control .
Experimental validation by semi-quantitative RT-PCR
1 μg RNA was used for cDNA synthesis with PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Cat#RR047A), and PCR was conducted with Premix Taq (TaKaRa Taq Version 2.0 plus dye,Cat#RR901A) in a total 30 μl reaction volume with UBQ as the positive control. All the products from AS and NATs were validated with 1% agarose gel electrophoresis.
- Ces A:
Cellulose synthase genes
cis-nature antisense transcripts
Cellulose synthase-like genes
Differentially expressed genes
False discovery rate
FLOWERING LOCUS C
Fragments per kilobase of transcript per million mapped reads
Long noncoding natural antisense transcripts
Single-molecule real-time sequencing
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We are thankful to Dr. Chen Can in College of Forestry, Fujian Agriculture and Forestry University for detecting chlorophyll and Photosynthetic rate.
This work was supported by the National Key Research and Development Program of China (2018YFD0600101 and 2016YFD0600106), the National Natural Science Foundation of China Grant (Grant No. 31570674), the Natural Science Foundation of Fujian Province, China (Grant No. 2018 J01608), Fujian Innovative Center for Germplasm Resources and Cultivation of Woody plants (no. 125/KLA15001E), and the International Science and Technology Cooperation and Exchange Fund from Fujian Agriculture and Forestry University (KXGH1701).
Availability of data and materials
All the RNA-Seq raw data are available at NCBI under accession number GSE104596. The bigwig files have been uploaded to our website: http://www.forestrylab.org/pub/GA.
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The authors declare that they have no competing interests.
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Table S2. Summary of RNA-Seq mapping. (XLSX 30 kb)
Table S3. List of significant differentially expressed genes. (XLSX 1396 kb)
Table S1. Primer list used in this study. (XLSX 20 kb)
Table S4. GO analysis of DEGs. (XLSX 93 kb)
Table S5. List of 188 significantly altered DEGs engaged in eight principal classes of plant hormones. (XLSX 38 kb)
Table S6. List of differentially AS events including IR, ES, AltA and AltD. (XLSX 207 kb)
Table S7. List of hormone-related and splicing factor genes, which have different isoforms after GA was applied. (XLSX 31 kb)
Table S8. List of cis-NATs in moso bamboo, which were classified into head-to-head (5′-5′), tail-to-tail (3′-3′) and fully overlapping. (XLSX 156 kb)
Table S9. List of 42 differentially expressed cis-NATs upon GA treatment. (XLSX 14 kb)
Table S10. GO analysis of 42 differentially expressed cis-NATs. (XLSX 9 kb)
Table S11. List of 132 differentially expressed TFs. (XLSX 82 kb)
Table S12. List of TFs associated with AS and cis-NATs. (XLSX 31 kb)
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Cite this article
Zhang, H., Wang, H., Zhu, Q. et al. Transcriptome characterization of moso bamboo (Phyllostachys edulis) seedlings in response to exogenous gibberellin applications. BMC Plant Biol 18, 125 (2018). https://doi.org/10.1186/s12870-018-1336-z
- PacBio single molecule real time sequencing
- Nature antisense transcripts
- Alternative splicing
- Moso bamboo