Fig. 6
Five genes with AS events were affected by GA3. a-c. Wiggle track for three candidate genes, which have differential isoforms after GA3 treated. Gene structures in blue originated from Pacbio SMRT dataset, while green were generated by Cufflinks assemble of RNA-Seq. “P” indicates the AS events predicted with Pacbio SMRT data, and “C” represented events from RNA-Seq data. d. AS events from five genes were validated by RT-PCR gel blot with UBQ as the positive control. “+” indicated GA treatment, and “-” represented non-treatment. e. Validation of AS events by qRT-PCR