- Research article
- Open Access
Gene loss and genome rearrangement in the plastids of five Hemiparasites in the family Orobanchaceae
BMC Plant Biology volume 18, Article number: 30 (2018)
The chloroplast genomes (plastome) of most plants are highly conserved in structure, gene content, and gene order. Parasitic plants, including those that are fully photosynthetic, often contain plastome rearrangements. These most notably include gene deletions that result in a smaller plastome size. The nature of gene loss and genome structural rearrangement has been investigated in several parasitic plants, but their timing and contributions to the adaptation of these parasites requires further investigation, especially among the under-studied hemi-parasites.
De novo sequencing, assembly and annotation of the chloroplast genomes of five photosynthetic parasites from the family Orobanchaceae were employed to investigate plastome dynamics. Four had major structural rearrangements, including gene duplications and gene losses, that differentiated the taxa. The facultative parasite Aureolaria virginica had the most similar genome content to its close non-parasitic relative, Lindenbergia philippensis, with similar genome size and organization, and no differences in gene content. In contrast, the facultative parasite Buchnera americana and three obligate parasites in the genus Striga all had enlargements of their plastomes, primarily caused by expansion within the large inverted repeats (IRs) that are a standard plastome feature. Some of these IR increases were shared by multiple investigated species, but others were unique to particular lineages. Gene deletions and pseudogenization were also both shared and lineage-specific, with particularly frequent and independent loss of the ndh genes involved in electron recycling.
Five new plastid genomes were fully assembled and compared. The results indicate that plastome instability is common in parasitic plants, even those that retain the need to perform essential plastid functions like photosynthesis. Gene losses were slow and not identical across taxa, suggesting that different lineages had different uses or needs for some of their plastome gene content, including genes involved in some aspects of photosynthesis. Recent repeat region extensions, some unique to terminal species branches, were observed after the divergence of the Buchnera/Striga clade, suggesting that this otherwise rare event has some special value in this lineage.
Beyond its essential roles in photosynthesis, the chloroplast is also the specialized site for synthesis of some pigments, lipids, amino acids, and sulfur compounds [1, 2]. The chloroplast contains its own genome, the plastome. The plastome in vascular plants is believed to have a single endocytotic origin, more than a billion years ago, from a cyanobacterium-like organism [3,4,5]. Over time, most genes of the cyanobacterium have either been lost or have migrated to the nuclear and/or mitochondrial genomes, so that modern plant plastomes contain approximately 10% of the ancestral endosymbiont genes .
Plastomes are under strong selective pressure in most plants and therefore tend to be highly conserved in terms of gene content and order, nucleotide substitution rates, structure, and size . Most plastomes range between 120 to 160 kb in size . Originally, plastomes were thought to be arranged as single circular molecules. Although a percent of plastomes do have this structure, they are also present in dimers and higher order concatemers, in both circular and linear configurations . Plastomes have a quadripartite structure consisting of a large single copy (LSC) region and a small single copy region (SSC) separated by two virtually identical inverted repeat (IRA and IRB) regions . Recombination between the two inverted repeats plays a role in stabilizing the plastome . In angiosperms, LSCs are typically 80–90 kb, SSCs 16–27 kb, and inverted repeats 20–30 kb . The plastome contains genes encoding proteins involved in photosynthesis as well as the other biochemical pathways carried out in the chloroplast. It also encodes 30 tRNAs and several other structural RNAs [10,11,12].
Unlike most species of plants, parasitic plants often contain highly divergent plastomes [13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28]. Non-photosynthetic plants that obtain all their nutrients from the host plant are called holoparasites, while hemiparasites are photosynthetic plants that obtain some nutrients from the host and the rest from photosynthesis. Hemiparasites can be further broken down into obligate parasites that require a host plant during at least part of their life cycle or facultative parasites that are capable of completing their entire life cycle without a host plant, but can parasitize if a host is available. Parasitic plant plastomes can have lower selective pressure due to less reliance on photosynthesis. Not surprisingly, holoparasites have the potential to lose all of their photosynthetic genes, or perhaps the entire chloroplast genome , while hemiparasites are expected to maintain most or all of their photosynthetic genes. Lack of or less selection for retained photosynthetsis and other vital chloroplast functions can allow higher nucleotide substitution rates and rearrangements in the plastome. These changes can include functional gene loss through pseudogenization or physical loss through gene deletion, resulting in a smaller plastome size [14, 16, 17, 19,20,21,22,23, 26]. In some cases, an entire copy of one of the inverted repeats has been lost [19, 20, 29]. Since the repeats play a stabilizing role in plastome structure, this leads to further destabilization and rearrangements in the plastome .
The Orobanchaceae are the largest family of parasitic plants. They contain a single non-parasitic genus, Lindenbergia , in addition to holoparasites and both facultative and obligate hemiparasites . In this study, we looked at two species of facultative hemiparasites, Aureolaria virginica and Buchnera americana, and three species of obligate hemiparasites all in the genus Striga: S. hermonthica, S. forbessii, and S. aspera. The plastome of the autotroph Lindenbergia philippensis had already been fully sequenced and annotated by Wicke et al. 2013, so it provided a valuable outgroup to compare to our parasitic species. Despite the fact that all of our studied species rely on photosynthesis, we discovered numerous cases of gene loss and other forms of rearrangement in their plastomes.
Sample collection, DNA extraction and DNA sequencing
DNA preparations from leaf tissue of Striga species were the same as those previously described . B. americana and A. virginica leaf tissues were collected from live specimens in the Plant Biology Teaching collection at the University of Georgia. High molecular weight DNAs were isolated from these samples by a modified cetyltrimethylammonium bromide procedure  and were used for Illumina library preparation with the NEBNext® Ultra™ II DNA Library Prep Kit as per manufacturer’s instructions. Illumina MiSeq PE250 runs were performed at the Georgia Genomics Facility.
Plastid-homologous sequences were selected from the total cellular DNA reads of Striga hermonthica and then assembled with a de novo assembly pipeline, both as previously described , to create ten contigs. To fill the ten gaps in this assembly, primers were designed for the end of each contig and PCR was set up for each primer pair. Each PCR reaction consisted of 5–20 ng template DNA, 10 μL Q5 Reaction Buffer, 10 μL betaine, 2.5 μL DMSO, 1 μL 10 mM dNTPs, 2.5 μL forward primer, 2.5 μL reverse primer, .5 μL Q5 High-Fidelity DNA Polymerase, and 20 μL water. Touchdown PCR was performed on each reaction on an MJ Research PTC-200 Peltier Thermal Cycler. The program used was 98 °C for 30s; 10 cycles consisting of denaturation at 98 °C for 20 s, annealing at 68 °C - 1 °C each cycle for 15 s, elongation at 72 °C for 1 min; 25 cycles consisting of denaturation at 98 °C for 15 s, 58 °C for 15 s, elongation at 72 °C for 1 min; and a final elongation step at 72 °C for 10 min. PCR products were sent to Macrogen USA (Rockville, MD) for Sanger sequencing. Sequences were manually assembled to the original contig ends in order to fill gaps.
The assembled S. hermonthica plastome was aligned to the Lindenbergia philippensis plastome using Mauve 2.3.1 . All junctions to predicted rearrangements in the S. hermonthica plastome were PCR amplified and Sanger sequenced (Macrogen USA) to confirm that the assembly was correct.
Plastome reads for each of our target species were extracted from raw total DNA reads by identifying reads homologous to our S. hermonthica assembly and used in de novo assemblies with Geneious 8.1.6 . Hence, each de novo assembly was performed independently for each species.
The repeat region of each assembly was identified by the region of the plastome having approximately two times the read coverage compared to the rest of the plastome. In the case of S. hermonthica, we ran PCR on all the junctions between the repeats and the LSC and SSC. Sequencing of these PCR products confirmed the assembly of these junctions.
Plastome annotation and alignment
The genes from the annotated L. philippenis plastome were downloaded from NCBI along with the plastome genes from all plant species from Chloroplast Genome DB . We ran BLAST on all genes against each assembled plastome. All genes identified in the plastome were then mapped to the plastome using Geneious. Reads were also mapped to each gene individually to confirm that the sequence assembly was correct and that there were no genes missing in our assemblies that had reads map to them. Individual genes were aligned across all five parasitic species as well as L. philippensis using Clustal W  to identify mutations, insertions, and deletions. Genes that contained one or more frameshift mutations or premature stop codons were considered potential pseudogenes. The raw reads for the most 5′ predicted stop codons or frameshift mutations that could have created a pseudogene were visually inspected to evaluate the accuracy of the read call. Read depth varied from 34X to 548X across these sites, with 18 cases giving 100% agreement with the mutational call, and the others showing > 95% agreement (data not shown).
The plastomes were aligned using Mauve 2.3.1. The phylogenetic tree was generated with sequence data from all intact genes shared by all five species. These genes were concatenated into a single sequence, which were aligned using Clustal W in Mega 7.02 . L. philippensis was included as an outgroup. Bayesian phylogenetic analyses were done on MrBayes 3.2.1  using Generalized Time Reversible + Γ + I model of evolution. We ran an initial 2.0 × 107 trees with sampling every 10,000 generations. The first 5.0 × 106 trees were not included in the final analysis. The remaining trees were used to generate a consensus tree. The same data were used to generate a phylogenetic tree using MEGA , and this generated nearly identical results, so only the MrBayes tree is presented.
Illumina sequence analysis of total DNA from leaves provided sufficient plastome-homologous sequence data for full genome assembly of all five studied species. All of the initial contiguous sequences were confirmed in their order, and gaps filled, by PCR and Sanger sequencing of the PCR product. Hence, complete assemblies were obtained, and any predicted rearrangements by comparisons of these species genomes were also confirmed by PCR and Sanger sequencing.
Our assemblies indicated that the plastome of A. virginica is 153,547 bp, close in size to the 155,103 bp plastome of L. philippensis. The lengths of the LSC, SSC, and IR (84,317 bp, 17,168 bp, and 26,031 bp, respectively) in A. virginica were all similar in size to those in L. philippensis (85,584 bp, 17,885 bp, and 25,812 bp, respectively). The plastomes from the other four species (S. hermonthica, S. aspera, S. forbessii and B. americana) showed major differences in both total plastome size and the size of each region.
The three Striga species and B. americana all exhibited larger plastome sizes, ranging from 166,596 bp in B. americana to 190,233 bp in S. forbessii. The larger plastome sizes correlate with the larger IR lengths, ranging from 43,864 bp in B. americana to 63,240 bp in S. forbessii. Conversely, the LSC and SSC regions in all four species showed a reduction in size. SSC lengths ranged from 3377 bp in Buchnera to 11,191 bp in S. forbessii, while the LSC lengths ranged from 51,628 bp in S. hermonthica to 75,491 bp in B. americana. All plastome component sizes are shown in Table 1, and are compared to those for some previously studied parasitic plants.
In B. americana, repeat expansion has occurred in both the LSC and SSC regions. Approximately 8 kb of LSC sequence flanking the repeat in L. philippensis is now included in the repeat region in B. americana. This region contains the genes trnH, psbA, matK, trnK, rps16, trnQ, and psbK. The expansion also occurred into both flanking regions of the SSC. On one end of the SSC, ndhF has been lost in B. americana while the adjacent 6 kb region is now part of the repeat region. ndhE, psaC, ndhD, ccsA, trnL, and rpl32 are all contained within this region. On the other end of the SSC, an approximately 5 kb region containing rpl32 and ycf1 has been shifted into the repeat in B. americana.
The three Striga species show an almost identical pattern of IR expansion, with the majority of expansion occurring in the LSC region. The two LSC-flanking regions as well as an internal region are now part of the repeat in all three Striga species. An approximately 12.5 kb region flanking the IR in L. philippensis is part of the IR in Striga. This region contains trnH, psbA, matK, trnK, rps16, trnQ, psbK, psbI, trnS, trnG, trnR, and atpA. On the other end of the LSC, the IR region has expanded into approximately 18.8 kb of adjacent sequence. This region contains 15 genes: clpP, psbB, psbT, psbN, psbH, rpoA, rps11, rpl36, infA, rps8, rpl14, rpl16, rps3, rpl22, and rps19. The internal region is 5.5 kb and contains ycf4, cemA, petA, psbJ, psbL, psbF, and psbE. In L. philippensis, there are 61 kb between this region and the IR on one side and 18 kb between this region and the IR on the other side. Approximately 4.5 kb of the SSC region adjacent to the repeat is now part of the repeat region in all three Striga species. This region contains the ycf1 gene. All details of repeat expansion are shown in Table 1.
Overall gene content
No tRNA or rRNA genes have been lost in any of the five species. All of the plastomes encode 30 different tRNAs and 4 different rRNAs. Compared to the outgroup Lindenbergia, Aureolaria has not lost any protein-coding genes and contains a total of 79 single copy genes and six more that are contained within the repeat region and therefore present in two copies. Buchnera has a total of 77 single copy genes, plus 17 in the repeat regions, with 9 genes being possible pseudogenes. S. forbessii contains 79 single copy genes, plus 35 contained within the repeats, including 14 potential pseudogenes. S. hermonthica has 76 single copy protein-coding genes, 35 in the repeats and 10 potential pseudogenes. S. aspera contains 77 single copy genes, plus 35 in the repeat regions, with 12 potential pseudogenes (Table 2).
In L. philippensis and A. virginica, ndhA is a 2.2 kb gene containing two exons, 539 bp and 553 bp, separated by a 1.1 kb intron. S. hermonthica is missing the second exon of ndhA, while S. forbessii is missing the first exon. B. americana and S. aspera have both exons, but have a predicted frameshift mutations and premature stop codons in the first exon. All ndh genes are present with the structure of functional genes in A. virginica. B. americana and the three Striga species all have one or more stop codons or frameshifts in ndhB, starting at amino acid 446 in B. americana and 448 in S. forbessii. S. hermonthica and S. aspera both have a frameshift at amino acid 24, suggesting a shared event in their common ancestor, followed by multiple frameshifts and stop codons. ndhC is present in B. americana but contains a stop codon at amino acid 82. S. forbessii contains ndhC with a frameshift starting at amino acid 23 followed by multiple stop codons. ndhC is missing from both S. hermonthica and S. aspera. All four species contain multiple frameshifts and stop codons throughout ndhD starting at amino acid 36 in B. americana, 25 in S. forbessii, 4 in S. hermonthica and 40 in S. aspera. S. forbessii contains a stop codon in ndhE at amino acid 101. B. americana has a stop codon at amino acid 11. S. hermonthica has a stop codon at amino acid 40. B. americana, S. hermonthica, and S. aspera all contain 5′ frameshift mutations, at amino acid 34 in B. americana and amino acid 32 in the Striga species, but all are independent events. All three species have additional stop codons following the beginning of the frameshift. ndhF is missing from B. americana and contains a frameshift at amino acid 20 in S. forbessii followed by multiple stop codons. S. hermonthica and S. aspera both contain a stop codon at the third amino acid followed by additional stop codons and frameshifts. ndhG contains a frameshift and multiple stop codons starting at amino acid 63 in B. americana and amino acid 3 in S. forbessii. ndhG is missing from S. hermonthica and S. aspera. ndhH is missing from B. americana, S. hermonthica, and S. aspera. ndhH is present in S. forbessii but contains frameshifts and multiple stop codons starting at amino acid 11. ndhI contains a stop codon in B. americana at amino acid 90 and in S. forbessii at amino acid 17, followed by a frameshift and additional stop codons. S. hermonthica contains a frameshift starting at amino acid 47 and S. aspera contains a frameshift starting at amino acid 42, both followed by multiple stop codons. ndhJ contains stop codons starting at amino acid 140 in B. americana. In S. forbessii, ndhJ contains a frameshift at amino acid 71 followed by multiple stop codons. ndhJ in S. hermonthica contains stop codons starting at amino acid 102. ndhJ contains a frameshift in S. aspera starting at amino acid 44 followed by stop codons. ndhK contains a stop codon at amino acid 120 in B. americana and amino acid 14 in S. forbessii. Both species contain additional stop codons and S. forbessii contains a frameshift. ndhK in both S. hermonthica and S. aspera contain a frameshift at amino acid 4 followed by stop codons. Pertinent frameshifts and stop codons are shown in Fig. 1. Table 3 shows mutations shared by two or more species at the identical amino acid that resulted in frameshifts or stop codons.
accD is present in all five species. In A. virginica, it appears functional with very few differences in comparison to L. philippensis. In the other four species, there are a numerous predicted mutations including multiple frameshifts. However, the 3′ end of this gene is considerably more conserved than the 5′ end of the gene. It’s possible that accD has a conserved protein domain encoded by the 3′ end of the gene. ycf1 and ycf2 also seem to have multiple frameshift mutations in all species, except ycf2 in A. virginica.
Plastome alignments and phylogenetic analysis
All six plastomes were aligned with Mauve 2.3.1 and are shown in Fig. 2a. A. virginica is largely identical to L. philippensis, except that the SSC is in an inverted orientation. All other plastomes contained multiple rearrangements. Within the Striga genus, S. hermonthica and S. aspera have no rearrangements between them, while S. forbessii has a single rearrangement within the repeat region. At a higher resolution, Fig. 2b shows the example of the Mauve alignment of just S. hermonthica and A. virginica.
The timing of gene loss events
In many cases, a specific nucleotide location of a sequence change that is predicted to inactivate a gene was shared by more than one species. Hence, it is most likely that such events occurred in a common ancestor of the species that share the event. Because these deletions, frameshifts and/or stop codon creations are predicted to inactivate the gene, then it is appropriate to plot the lineage and relative time of inactivation of each gene onto a phylogenetic tree of the compared species. The phylogenetic tree, with L. philippensis as an outgroup, is shown in Fig. 3, with predicted shared gene inactivations indicated as gene names along specific branches.
Plastome conservation and change
Plastomes perform common and vital functions in virtually all vascular plants, and thus are very powerful tools for phylogenetic analysis. The parasitic plants that are non-photosynthetic lose strong selection for the many plastome genes involved in photosynthesis, but are expected to retain coding capacity for genes that are involved in other essential chloroplast roles. In previous studies of non-photosynthetic plants [13, 14, 18,19,20,21], most or all photosynthetic genes are seen to be pseudogenized or lost. This process appears to be caused by random mutation, and is thus thought to be a gradual process that will only be completed at some distant time after the parasitic/non-photosynthetic lifestyle is established. Our observations, akin to those observed by Wicke et al.  in a broader but less detailed survey of parasitic plants, indicate that some chloroplast genes are lost even in fully photosynthetic parasites, suggesting that chloroplast roles other than generation of photosynthate may now be provided by the host. Hence, extensive gene loss from the plastome does not link exclusively with a holoparastic lifestyle. If hemiparasitism is a routine step toward the evolution of holoparasitism, then it may be that the plastome gene loss process is as much a cause of holoparasitism as it is an outcome.
Parasitism has evolved independently at least 12 to 13 times within angiosperms . Parasitism within Orobanchaceae is believed to have evolved once and is estimated to have occurred 32–64 mya [43,44,45]. It is interesting that these different plastomes have come to somewhat different final gene contents and that many sequences are still present despite their loss of apparent function. This suggests that the gene loss process is very slow, especially compared to nuclear DNA loss , and raises the possibility that the different adaptations of these different species has led to different plastome gene loss outcomes. For instance, most ndh genes were lost early in the lineage leading to the genus Striga, but ndhE, ndhH and ndhJ have only been lost in terminal branches, suggesting a possible transitional role for these genes even when the other ndh genes were no longer functional. Similarly, Pedicularis cheilanthifolia has apparently lost only one plastome gene to pseudogenization, ndhf, a gene that has been independently lost in many lineages . All these results confirm the observation that, in hemiparasites, the ndh pathway is unnecessary or possibly even selected against. Further analysis of the nature of plastome genome change should provide additional insights into which genetic (and, thus, physiological) functions are lost, in what lineages and at what evolutionary rates and times.
Most plant plastomes range in size from 120 to 160 kb. L. philippensis falls within this range. The A. virginica plastome is slightly smaller than that of L. philippensis, but still falls within the typical range. The LSC, SSC, and IR of A. virginica are all similar in size to those of L. philippensis and fall within the general size range for angiosperms. B. americana and the three Striga species, however, all show interesting size difference in both whole plastome length and the lengths of their LSCs, SSCs, and IRs. All have a larger plastome than L. philippensis and above the range generally seen in plants. This was unexpected as most parasitic species studied so far have smaller plastome sizes [14, 16, 17, 19,20,21,22,23]. The increase in size can be explained by an increase in the length of the inverted repeats. All five species have an increased inverted repeat size, although Aureolaria’s is close to Lindenbergia (26,031 bp compared to 25,812 bp) and falls within the typical range of angiosperm inverted repeat size. The other four species have considerably larger repeats above the 20 to 30 kb size generally seen in angiosperms, ranging from 43,864 bp in Buchnera to 63,240 bp in S. forbessii. Small changes of a few hundred bp in inverted repeat size are common in land plants [47,48,49], but larger changes as seen here are rare [47,48,49,50,51,52]. The largest known repeat belongs to Pelargonium hotorum, at 75,741 bp . Slight IR expansion has been seen in the parasitic plastomes of Schwalbea americana and Cistanche phelypaea. However, these expansions were much smaller in size, encompassing 2 genes and 3 genes , respectively, compared to the expansions we saw in Striga and B. americana.
The repeat size increase that we observed is due to an expansion into the single copy regions. All five parasitic species have smaller SSC and LSC regions compared to L. philippensis. The Striga expansions differ dramatically from the expansion in B. americana. In B. americana, most of the repeat expansion has been into the SSC region. In the three Striga species, there has been some repeat expansion into the SSC region, but most of the expansion has been into the LSC region. There are two similarities in the expansion patterns between the Striga species and Buchnera. The first is the 8 kb in the LSC region adjacent to the IR (spanning from trnH to psbK). This region is contained within the repeat of all four species, although the Striga IRs extend a further 4.5 kb region into this region. It is possible that the initial 8 kb expansion occurred before the species diverged and was followed by an additional 4.5 kb expansion in the Striga lineage. The second similarity is the expansion into the SSC that contains the ycf1 gene. The B. americana IR includes an additional 0.5 kb containing rpl32, suggesting that this was an additional expansion after the lineages diverged. Other than these two similarities, the rest of the repeat expansions are unique to either B. americana or the Striga species and most likely occurred after the two lineages diverged. These several independent repeat expansions indicate either selection for this phenomenon or a mechanistic anomaly in these lineages that makes this outcome unusually likely, compared to other studied plants. Functionally, these increases in the duplicated regions do not appear to have created any new genes. Because of the process of gene conversion across these recombining repeats , it is not expected that any new gene functions would evolve through this IR expansion.
S. aspera and S. hermonthica show no rearrangements between them or differences between the repeat expansions. There is one major structural difference between the plastomes of these two and that of S. forbessii. Within the repeat, a 2.5 kb region containing cemA and petA is located in a different part of the region in an inverse orientation. One possibility is that repeat expansion occurred independently in the S. forbessii lineage and the shared S. hermonthica/aspera lineage after the two lineages diverged. The other possibility is that the repeat expansion occurred before the common ancestor of these three species diverged, followed by lineage-specific rearrangement(s) after divergence.
The NAD(P)H-dehydrogenase complex is involved in one of the multiple pathways that recycles electrons around photosystem I [54, 55, 60]. The NAD(P)H complex functions under stressful conditions and may be essential for photosynthesis under conditions of highly variable light intensities [56, 57]. Eleven subunits are encoded by the plastome: ndhA, B, C, D, E, F, G, H, I, J, and K . These genes have been lost several times in land plants and are commonly missing in parasitic plants [13, 16, 17, 19, 20, 23, 59,60,61,62,63].
Ycf1was the first plastid-encoded protein identified whose presence was shown to be essential for the survival of green plants. Ycf1 protein is an essential component of the translocon that is responsible for chloroplast protein transport in green plants . However, no ycf1 homolog was detected among Poales, except Typha latifolia . In our five studied species, ycf1 was observed to be highly divergent, with predicted frameshift mutations. The gene ycf2 still has an unknown function  and has a functional structure in A. virginica, but contains multiple frameshift mutations in B. americana and the three Striga species. Both ycf1 and ycf2 have high substitution rates in most of the land plant, including non-parasites, and may have become pseudogenes in some lineages [67, 68]. The 5′ ends of both genes tend to be relatively conserved, while the remaining portions of the genes are more divergent. We observed a similar pattern with the most conserved regions of both genes being in the 5′ end. Complete losses of both ycf1 and ycf2 have occurred in some monocots .
AccD encodes the beta subunit of the multimeric acetyl-CoA carboxylase, which mediates the conversion of acetyl-CoA to malonyl-CoA during fatty acid synthesis. The accD gene has been lost several times in angiosperms , where its function is taken over by nuclear copies . The 3′ region of the accD gene in all five of our studied species is considerably more conserved than the 5′ end. Higher divergence or truncation of the 5′ end of the accD gene also has been observed in several species [19, 20]. We do not have a nuclear genome sequence for any of the species investigated in this manuscript, but we predict that a nuclear accD homologue is now functional in the B. americana and Striga lineages. We also predict that the 3′ end of the accD gene may have a separate, plastid-specific, function.
A. virginica has no major genic rearrangements compared to L. philippensis except an inverted SSC region. The SSC region is frequently flipped in plastomes, with opposite SSC orientations often present in a 50:50 ratio in plant cells . Comparisons between A. virginica and B. americana are interesting as they are both facultative hemiparasites. Because they are both capable of living without a host, thereby depending heavily on photosynthesis, it is expected that they would have a relatively small number of rearrangements compared to autotrophic plant plastomes. This is the case with A. virginica. However, B. americana has multiple rearrangements compared to A. virginica and L. philippensis. The three Striga species also have several rearrangements compared to A. virginica and L. philippensis, as well as compared to B. americana. There are no rearrangements between S. hermonthica and S. aspera, and a single rearrangement between these two and S. forbessii. These patterns all fall within an appropriate phylogenetic order that predicts their time and lineage of origin.
The phylogenetic tree agrees with existing phylogenetic analysis as far as the relationship between L. philippensis, A. virginica, B. americana, and the Striga genus , as well as the relationship of the three Striga species to each other with S. hermonthica and S. aspera being more closely related to each other than to S. forbessii .
Plastome instability and parasitic plant function
Although all five of the plant species investigated in this study are obligate photosynthetic organisms, plastome instability was found to be extensive in four species. These results are in agreement with previous observations for other parasitic plants [13,14,15,16,17,18,19,20,21], and has also been seen more rarely in non-parasites [29, 45, 53, 65, 73,74,75,76,77,78,79]. Hence, plastome instability is a source of genomic variation that provides the raw material for natural selection. In any green plant, this selection is expected to lead to overall conservation of gene content, with exceptions for any chloroplast function that can be provided by the environment directly. For parasitic plants, the host plant is a primary environmental contributor, potentially for photosynthate and many of the numerous small molecules that are generated by pathways localized to the chloroplast. The ndh pathway is lost in many lineages, suggesting that its role in photosynthesis is often dispensable. Given the rapid and near-complete loss of this pathway independently in so many lineages, it suggests that ndh may actually be selected against in some plant lineages. Future studies that return this pathway to a species from which it has been naturally lost would provide an excellent opportunity to investigate the lineages and conditions under which the ndh gene array might benefit or debilitate a plant.
In this same vein, all of the gene changes observed in this study could be investigated for their role in plant fitness. Expression levels of the genes that differ in their copy number across these taxa, for instance due to their inclusion or lack of inclusion in the IRs, could suggest a role for the generation and retention of such rearrangements across many plant lineages. Natural variation for plastome structure should be tested across more Orobanchaceae species, both to acquire better ideas of the lineages and rates of such rearrangements, but also to provide the raw material for comparisons of the functional outcomes of these rearrangements. With the ability to transform chloroplasts now available in many plant species [80,81,82,83], investigations of natural plastome variation and its role in organismal function could be directly compared to experiments that test these gene effects in engineered transgenics.
We sequenced and assembled the chloroplast genomes from five species of hemiparasitic plants from the Orobanchaceae. We compared the assemblies to the available plastome assembly from Lindenbergia philippensis, an autotroph from the Orobanchaceae. Aureolaria virginica was almost identical to L. philippensis in terms of plastome structure, gene content, and gene order. B. americana, S. forbessii, S. hermonthica, and S. aspera all showed a high number of rearrangements and both physical and functional gene loss. The most interesting result was the increase in the plastome size in these four species, since most parasites have plastomes with decreased sizes. The increase was due to the repeat region expanding into the single copy regions, although what effect this has on the plastome is not yet known. B. americana, S. hermonthica, and S. aspera all had missing ndh genes, while these three species and additionally S. forbessii had multiple ndh genes that are potentially nonfunctional due to predicted frameshift mutations and stop codons. No other genes were missing from any of the species, but accD, ycf1, and ycf2 all had high levels of divergence with frameshifts in B. americana, S. forbessii, S. hermonthica, and S. aspera, as well as stop codons in ycf1 and ycf2 in S. hermonthica and S. aspera.
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We thank Prof. J. Palmer (Indiana University) and Prof. S. Wicke (University of Muenster) for their helpful comments.
This research was supported by USDA/NIFA grant award #2012–67013-19473 and the endowment associated with the Giles Professorship in the Department of Genetics at the University of Georgia.
Availability of data and materials
The datasets generated and/or analyzed during the current study are available in the GenBank repository, https://www.ncbi.nlm.nih.gov/genbank/. with accession numbers MF780870, MF780871, MF780872, MF780873, and MF780874.
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Frailey, D.C., Chaluvadi, S.R., Vaughn, J.N. et al. Gene loss and genome rearrangement in the plastids of five Hemiparasites in the family Orobanchaceae. BMC Plant Biol 18, 30 (2018). https://doi.org/10.1186/s12870-018-1249-x
- Chromosome rearrangement
- Gene deletion