- Research article
- Open Access
Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
BMC Plant Biology volume 18, Article number: 29 (2018)
Terminal drought stress leads to substantial annual yield losses in chickpea (Cicer arietinum L.). Adaptation to water limitation is a matter of matching water supply to water demand by the crop. Therefore, harnessing the genetics of traits contributing to plant water use, i.e. transpiration rate and canopy development dynamics, is important to design crop ideotypes suited to a varying range of water limited environments. With an aim of identifying genomic regions for plant vigour (growth and canopy size) and canopy conductance traits, 232 recombinant inbred lines derived from a cross between ICC 4958 and ICC 1882, were phenotyped at vegetative stage under well-watered conditions using a high throughput phenotyping platform (LeasyScan).
Twenty one major quantitative trait loci (M-QTLs) were identified for plant vigour and canopy conductance traits using an ultra-high density bin map. Plant vigour traits had 13 M-QTLs on CaLG04, with favourable alleles from high vigour parent ICC 4958. Most of them co-mapped with a previously fine mapped major drought tolerance “QTL-hotspot” region on CaLG04. One M-QTL was found for canopy conductance on CaLG03 with the ultra-high density bin map. Comparative analysis of the QTLs found across different density genetic maps revealed that QTL size reduced considerably and % of phenotypic variation increased as marker density increased.
Earlier reported drought tolerance hotspot is a vigour locus. The fact that canopy conductance traits, i.e. the other important determinant of plant water use, mapped on CaLG03 provides an opportunity to manipulate these loci to tailor recombinants having low/high transpiration rate and plant vigour, fitted to specific drought stress scenarios in chickpea.
Chickpea (Cicer arietinum L.), the second most important grain legume crops in the world , is widely cultivated on residual soil moisture in the arid and semi-arid agricultural systems of the world. Terminal water deficit is one of the major constraints limiting the chickpea crop productivity  and has been reported to cause yield losses upto 50% in chickpea .
Deeper and more profuse rooting has been hypothesized to be the major factor contributing to yield increase under water limited environments in chickpea, where the assumption was made that these root traits would increase water extraction [4,5,6,7,8]. However it was also shown that chickpea genotypes with deeper and more profuse rooting did not extract more water from the soil profile . Rather, tolerant chickpea genotypes turned out to be those able to somewhat limit water use at vegetative stage and making more water available for the grain filling period [9, 10]. Similar results have been reported in other crops (e.g. in pearl millet , in sorghum ). Therefore, the central hypothesis of the present study is that, given the limited seasonal water budget, any trait allowing water conservation during vegetative growth (e.g. canopy conductivity, canopy size and development) extends the duration of water extraction during pod filling and so increases productivity of chickpea crop under terminal water stress [9,10,11, 13,14,15].
In chickpea, the availability of large scale genomic resources has paved the way to dissect the mechanisms underlying various stresses adaptations [16, 17]. A recent mapping study in chickpea reported a genomic region on CaLG04 referred as a “QTL hotspot” that harbours several drought tolerance traits including rooting depth . Introgression of this region into elite variety JG 11 improved yield under drought . This reported “QTL hotspot” region (spanning ~ 29 cM) was originally associated with seven SSR markers . Further, this “QTL-hotspot” region was refined to~ 14 cM, with additional 49 SNP markers,  using genotyping-by-sequencing (GBS). Skim sequencing (with bin used as markers, based on recombination break points) approach then allowed to fine map this region to ~ 300 Kb . An intriguing feature of the preliminary steps of this research was also the mapping of a major QTL for shoot weight on CaLG04, which co-mapped with a QTLs for root traits (depth and length density), from a study where these traits were assessed in PVC tubes . Interestingly, the percentage of phenotypic variation explained by this QTL was more for the shoot dry weight than for the root traits, suggesting that this QTL region was a QTL for vigour, but this hypothesis was not followed further.
In chickpea, the studies of physiological traits allowing water conservation (e.g. canopy conductivity, canopy size & development; [9, 10]) are very scarce, partially because an accurate assessment of leaf area is a rate limiting step. Recognizing this obstacle, a high throughput phenotyping platform was developed to measure canopy development traits . The high throughput platform was used to phenotype the RIL population (ICC 4958 × ICC 1882), from which the “QTL-hotspot” was reported, for plant vigour traits (leaf area, plant height, rate of leaf area increase) and water saving traits (conductance), as a mean to re-investigate the map location of these traits with regards to the QTL hotspot earlier identified .
Therefore, the overall objective of this study was: i) to assess the phenotypic variation in traits involved in the control of plant water use either from canopy development or canopy conductance, and explore their functional associations in a RIL mapping population previously used for mapping the “drought tolerance QTL” (ICC 4958 × ICC 1882), ii) to map these drought adaptive traits and assess their interactions, iii) to conduct comparative mapping study using differently saturated genetic maps.
Based on trait functionality, these were clustered (Clustering analysis) and grouped into two major clusters: (i) a cluster of plant vigour traits [Plant vigour score (VIG), 3D-leaf area (3D-L), projected leaf area (PL), plant height (PH), 3D-leaf area growth rate (3D-LG), projected leaf area growth rate (PLG), plant height growth rate (PHG), shoot dry weight (SDW), leaf area index (LAI), specific leaf weight (SLW) and specific leaf area (SLA)]; and (ii) a cluster of traits related to canopy conductance [Transpiration (T), evapotranspiration (eT), transpiration rate (TR), evapotranspiration rate (eTR) and the residuals between 3D and projected leaf area (R-3D/PLA, a trait that was interpreted to represent the canopy structure)] (Table 1; Additional file 1).
Plant vigour related traits
The two parental genotypes (ICC 4958 and ICC 1882), as well as RILs, showed significant differences in plant vigour traits in both years (Table 2). For example, 3D-Leaf area (3D-L) was among those showing the largest variation, i.e. a 5-fold range variation in both years (Fig. 1a & Table 2). Continuous variation and normal frequency distribution were found for plant vigour traits (Additional file 2 A, B, C &D). Additional file 3 A & B showed 3D-leaf area & plant height development dynamics of parental lines. The high vigour parent ICC 4958 had faster leaf area and plant height development (canopy development) than low vigour parent ICC 1882.
According to Rabinson et al. , heritability (h2%) is classified as low (0-30%), moderate (30-60%) and high (> 60%). Most of the plant vigour related traits had h2% in the range of 60- 90% (e.g. PH, 3DL, 3D-LG, SLW and SDW; Table 2). Among these, plant height (PH) and 3DL showed highest heritability [PH (87.5 and 88%) and 3DL (76 and 89%) in 2014 and 2015 respectively].
Canopy conductance traits
The two parental genotypes (ICC 4958 and ICC 1882) and RILs (progenies) showed significant difference for all canopy conductance traits (T, TR, eT, eTR & R-3D/PLA) in both years (2014-2015; Table 2). For example, T was one among those showing the largest phenotypic variation, i.e. a 5-fold range variation in both years (Table 2). In addition, TR also showed 2-fold range of variation (Fig. 1b). Continuous variation and normal frequency distribution was found for all traits (Additional file 2 E, F, G & H - data not shown for R-3D/PLA). Transpiration and 3D-leaf area were tightly correlated (r2 = 0.68) until the LAI reached a value of 1 (25 DAS). Thereafter, this relationship became much weaker (r2 = 0.22) when the plants reached an LAI between 1 and 2 (38 DAS; see Fig. 2 a & b). At this stage, TR became much more closely related to T (r2 = 0.92), whereas this relationship was weaker (r2 = 0.62) when the LAI was less than 1 (25 DAS; see Fig. 2 c & d). Hence it was interpreted that at a low LAI, leaf area was the main driver of T. By contrast, at a high LAI, TR was the main driver of T. Since the average VPD during the transpiration measurement was high (3.76 kPa), this was interpreted to be caused by TR differences under high VPD.
Most of the canopy conductance traits had low to medium (25 to 68%) heritability (e.g. TR, eT, eTR and R-3D/PLA), except T (high h2 range; 62 and 70% in 2014 and 2015 respectively) (Table 2).
Trait correlation and their relationships
Simple Pearson correlation analysis
Phenotypic correlation coefficients of ICC 4958 x ICC 1882 population are presented in Additional file 4. As expected there were strong relationships within both groups of traits, but also between traits across groups. As expected, 3DL, LAI and SDW (plant vigour traits) were positively correlated with T and eT (canopy conductance traits), whereas 3DL, PL, LAI and SLA were negatively correlated with TR (see Additional file 4). Interestingly, most plant vigour traits were negatively correlated with R-3D/PLA (Canopy structure). By contrast, R-3D/PLA was positively correlated with TR and eTR. A significant correlation was observed among plant vigour traits. For example, plant vigour score (VIG) was significantly correlated with PH (0.73, P = 0.0001), PHG (0.62, P = 0.0001), 3DL (0.46, P = 0.0001), LAI (0.47, P = 0.0001), SDW (0.44, P = 0.0001) (Additional file 4). In addition, a significant correlation was observed among canopy conductance traits. For example, TR was well correlated with eT (0.63, P = 0.0001), eTR (0.61, P = 0.0001) and T (0.44, P = 0.0001) (Additional file 4).
Principal component analysis (PCA)
A principal component analysis was used to identify the relationships between parameters, and group these in a more comprehensive manner. Three principal components (PC) explained 62% of the total variation observed among the RIL population, using BLUPs phenotypic data across years (Additional files 5 and 6). PC1 (34%) had a strong positive loading from SLA and a strong negative loading from 3DL (Additional file 6), which agrees well with the strong negative correlations between these traits (Additional file 4). PC2 (17%) had a strong positive loading from PL and 3DL (plant vigour traits), whereas most canopy conductance traits had a strong negative loading. This also agreed well with the strong negative correlations between plant vigour (PL and 3DL) and canopy conductance (TR and eTR) traits (Additional file 4). PC3 (13%) had a strong positive loading from VIG, PH, PHG, SLA and T, whereas growth rate traits (3D-LG. PLG), canopy structure (R-3D/PLA) and SLW had strong negative loading (Additional file 6). This agreed well with the strong negative correlations between most of the plant vigour and canopy structure (R-3D/PLA) traits (Additional file 4).
Plant vigour traits
QTL analysis for single locus
Plant vigour related traits mapped predominantly on CaLG04. One M-QTL [LOD 36.7 & PVE 53%] for VIG was identified in CaLG04 within the reported refined “QTL-hotspot” region (~ 300 Kb; Additional file 7). For 3DL, three M-QTLs were identified, all (LOD 6-10 & PVE 11-18%) being found in both years within the earlier reported refined “QTL-hotspot” region with the favourable allele from ICC 4958 (Additional file 7). For PL, three QTLs were identified within the “QTL-hotspot”, with the favourable allele from ICC 4958 (Additional file 7). Among these, one was a M-QTL (LOD 6 & PVE 11%) and the remaining two were minor QTLs with PVE 8-9% (Additional file 7). For LAI, one M-QTL (LOD 6 & PVE 11%) was identified within the “QTL-hotspot” with the favourable allele from ICC 4958 (Additional file 7). Two minor QTLs were identified in CaLG04 (2 QTLs) (Additional file 7). For SDW, one M-QTL (LOD 9 & PVE 18%) was found with favourable allele from ICC 4958 within the “QTL-hotspot” (Additional file 7), and one minor QTL was identified in CaLG04 (Additional file 7). For PH, three M-QTLs (LOD 20-22 & PVE 34-39%) were identified in CaLG04 within the “QTL-hotspot” with favourable allele from ICC 4958, and one minor QTL was identified in CaLG04 (Additional file 7). For PHG, three M-QTLs (LOD 7-14 & PVE 13-23%) were found in CaLG04 within the “QTL-hotspot” with favourable allele from ICC 4958, and one minor QTL was identified in CaLG04 (Additional file 7). For SLA, three minor QTL were found in CaLG04 (PVE 4-8%; Additional file 7). Few major and minor QTLs from other CaLGs of plant vigour related traits were presented in Additional file 7.
Interaction QTL analysis for multiple loci
Plant vigour and canopy conductance related traits, epistatic QTL (E-QTLs) interactions were analyzed using genotype matrix mapping (GMM). In this section, only selected strongest epistatic QTL (E-QTLs) interactions and high F values with RILs number higher than 10 are discussed (Additional file 8). Additional E-QTLs interactions (Lower F values and RIL number and PVE %) for plant vigour and canopy conductance related traits were found and are shown in Additional file 9. Many E-QTLs interactions were identified for plant vigour traits (VIG, 3D-L, PL, PH, 3D-LG, PLG, PHG, SDW, LAI, SLW and SLA) and these are listed in Additional file 8.
Single locus region explained from − 23% to 17% of the phenotypic variation (Additional file 8). For most of the plant vigour traits, the favourable allele was contributed by the high vigour parent ICC 4958, for instance a single locus QTL [13.5% by LG04, 68.09 (AA)] increased PH by 13.5%. Two loci interactions explained from − 23% to 15% of the phenotypic variations (Additional file 8). For instance, two loci interactions [LG07, 63.45 (AA) + LG04, 68.09 (AA)] increased PH by 15% with favourable alleles from ICC 4958. By contrast, two loci interactions [LG04, 99.17 (BB) + LG04, 68.09 (BB)] strongly decreased PHG by − 25% with favourable allele from ICC 1882 (Additional file 8). Three loci interactions explained from − 25% to 17% of the phenotypic variation (Additional file 8). For instance, three loci interactions [LG04, 68.09 (AA) + LG03, 13.00 (AA) + LG03, 3.08 (AA)] increased PH by 17% with favourable allele from high vigour parent ICC 4958. By contrast, three loci interactions [LG08, 51.27 (−) + LG06, 91.97 (BB) + LG04, 24.82 (BB)] increased SLA by 10% with favourable allele from low vigour parent ICC 1882 (Additional file 8 and Fig. 3).
Canopy conductance traits
QTL analysis for single locus
For TR, one M-QTL (LOD 5 & PVE 10%) was identified in CaLG03 (Additional file 7), and four minor QTL were distributed on CaLG01 (2 QTLs and PVE 5%), CaLG02 (1 QTL and PVE 5%), CaLG07 (1 QTL and PVE 5%; Additional file 7). For eTR, one M-QTL (LOD 6 & PVE 11%) was identified on CaLG04 with favourable allele from ICC 1882. This QTL was located just outside the “QTL hotspot” region (Additional file 7). Along with this, four minor QTL were distributed on CaLG04 (2 QTLs & PVE 5-8%) CaLG03 (1 QTL & PVE 8%) and CaLG07 (1 QTL & PVE 5%; see Additional file 7). For T, three minor QTLs were identified, two of these explaining 8-9% phenotypic variation on CaLG04 (“QTL-hotspot” region) with favourable allele from ICC 4958. Another one QTL for T was present in CaLG05 (PVE 5%) with favourable allele from ICC 1882 (Additional file 7). For eTR, four minor QTLs were distributed on CaLG04 (2 QTLs & PVE 6-7%), CaLG05 (1 QTL & PVE 5%) and CaLG06 (1 QTL & PVE 5%; Additional file 7).
Interaction QTL analysis for multiple loci
E-QTLs interactions identified for canopy conductance (T, TR, eT, eTR & R-3D/PLA) are listed in Additional file 8. Single locus region explained 2.2% to 20% of the phenotypic variation (Additional file 8). For instance, single locus [LG07, 39.00 (BB)] increased R-3D/PLA by 20% with the favourable allele from ICC 1882. Two loci interactions explained from − 43% to 8% of the phenotypic variations (Additional file 8). For instance, two loci interactions [LG07, 37.57 (AA) + LG04, 68.09 (AA)] decreased R-3D/PLA by 43% with favourable allele from ICC 4958. By contrast, two loci interactions [LG07, 12.46 (BB) + LG06, 56.51 (BB)] increased TR by 3.25% with favourable alleles from ICC 1882. Three loci interactions explained from 4% to 31% of the phenotypic variations (Additional file 8). For instance, three loci interactions [LG04, 39.08 (BB) + LG03, 09.09 (BB) + LG01, 16.65 (BB)] increased R-3D/PLA (canopy structure) by 31% with favourable alleles from ICC 1882 (Additional file 8 and Fig. 3). Similarly, a three loci interactions (LG06, 12.48 (BB) + LG04, 44.46 (BB) + LG01, 25.00 (BB)] increased TR by 6.5% with all favourable alleles from ICC 1882 (Additional file 8 and Fig. 3).
Co-localization of plant vigour and drought tolerance related traits
Map position of plant vigour traits reported here was compared to map position of roots and drought tolerance traits reported earlier [18, 20, 21]. With the low density marker map, plant vigour traits co-localized with several root traits [eg. root length density, root dry weight/total plant dry weight ratio); see  from the previously reported “QTL-hotspot” region (Fig. 4-I-A, B & C; Additional file 10).
Similarly, mapping with high density markers data (GBS) showed that plant vigour traits (VIG, 3DL, PL, PH, PHG, LAI and SDW) co-localized with previously identified drought tolerance related traits [roots traits (RLD, RSA, RTR), morphological traits (SDW, PHT, PBS), phenological traits (DF, DM), yield related traits (100 SDW, BM, HI, POD, SPD, YLD) and drought indices (DSI, DTI)] (see, ) on CaLG04, which gave also a refined “QTL hotspot” region (Fig. 4-II-A, B&C; Additional file 11).
Further co-mapping work was done with the ultra-high density bin marker data (skim sequencing approach). Here, plant vigour related traits (VIG, 3DL, PL, PH, PHG, LAI, SDW) co-localized with previously identified drought tolerance related traits [RLD, RTR%, SDW, PHT, DM, POD, 100SDW, HI and DC; see, ] on CaLG04 within the “QTL- hotspot” region (Additional file 7; Fig. 4-III-A, B & C).
Bin-map “QTL-hotspot” region a & b
PH, PHG &VIG had several M-QTLs (LOD 6-37 and PVE 11-53%), and these were identified in the fine mapped “QTL-hotspot”-“a” region (0.23 cM) on CaLG04. In the same region, PHT, POD, 100-SDW, RLD and DC traits were previously mapped by Kale et al. . Similarly, 3DL, LAI and SDW had several M-QTLs (LOD 5-10 and PVE 11-18%) that were mapped in another “QTL-hotspot”-“b” region (0.22 cM). In the same region, RTR and SDW traits were previously mapped .
Asserting QTL location and size in different genetic maps
Different density genetic maps showed QTLs for plant vigour traits on CaLG04 and co-located with the “QTL-hotspot” region. Their size within the “QTL- hotspot” region using the low density (29 cM size), high density (~ 15 cM size) and ultra-high density maps (“QTL-hotspot” a & b (see more details on ) together ~300Kb size) on CaLG04 is discussed in this section.
For plant vigour related traits (VIG, 3DL, PL, PH, PHG, LAI, SDW), 28 and 32 M-QTLs were mapped on the low and high density maps, and their size ranged from 1 cM to 8.0 cM on the low density map and 0.8 cM to 5.6 cM on the high density map. For the same traits, the 15 M-QTLs that were mapped using the ultra-high density marker map (Table 3) had a size ranging from 0.14 cM to 0.15 cM. For instance, Fig. 5-I, II, III, IV-A, B&C showed plant vigour traits (VIG, 3DL, PH and SDW) in three different genetic maps. It showed that gradually LOD and PVE increased with marker density and simultaneously QTL size decreased, being fine-tuned down to 300Kb with the ultra- high density marker map. More details on major and minor QTLs for plant vigour in different density genetic maps are presented in Additional files 7, 10 and 11. In addition, low density genetic map along with plant vigour traits QTLs position are shown in Additional file 12A.
For canopy conductance traits (TR, eTR, T, eT and R-3D/PLA), several QTLs were identified on different linkage groups (CaLG01, LG03, LG04, LG05, LG06, LG07 & LG08) across the genome. A total of 18 and 20 M-QTLs were mapped on different LGs using low and high density maps, respectively (see more details in Additional files 10 and 11). The QTL size ranged from 1 cM to 15.0 cM size in low density map and high density ranged from 0.3 cM to 5.0 cM size (Table 3). Two M-QTLs were mapped on CaLG03 (TR) and just outside the CaLG04 “QTL-hotspot” region (eTR) using ultra-high density map. The QTL size ranged from 0.08 cM (TR) to 0.48 cM (eTR) size (Table 3). For TR, three M-QTLs with 5-13 cM were identified on CaLG07 using low density marker (Table 3). In the high density marker, no M-QTL was detected for TR. But, six minor QTLs were identified on CaLG03 (2QTLs; 4.9-5.1 cM), CaLG07 (1QTL; 2.0 cM), CaLG06 (1QTL; 10.3 cM) and CaLG04 (2.3-11.9 cM; Additional file 11). On the ultra-high density map, one M-QTL for TR was mapped on CaLG03 (0.08 cM). For TR, mapping position varied between low and ultra-high density markers. This might be most of the similar alleles between CaLG03 and CaLG07 (Table 3). Similarly for R-3D/PLA (canopy structure), 10 M-QTLs were identified on different linkage groups [CaLG04 (7 M-QTLs; 1.0-8.0 cM), CaLG06 (2 M-QTLs; 11-15 cM) & CaLG07 (1 M-QTL; 2.0 cM)] using low density markers (Table 3 & see more details in Additional file 10). For high density markers, 13 M-QTLs were identified on different linkage groups [CaLG04 (7 M-QTLs; 0.3-1.3 cM), CaLG06 (2 M-QTLs; 2.6-4.2 cM), CaLG07 (2 M-QTLs; 2.4-2.6 cM) and CaLG01 (2 M-QTLs; 4.1-4.2 cM)] (Table 3 & see more details in Additional file 11). There was no QTL was detected with the ultra-high density markers map (Table 3). More details of canopy conductance traits major and minor QTLs in different density genetic maps are presented in Additional files 7, 10 and 11. In addition, low density genetic map along with canopy conductance traits QTLs position is shown in Additional file 12B, C & D.
The summary of the main results is as follows: i) Genetic variation of 16 phenotypic traits revealed two clusters of plant vigour and canopy conductance traits and their association was clarified with PCA analysis and correlation. ii) Using the ultra-high density map, M-QTLs for plant vigour traits predominantly mapped on CaLG04 and these co-mapped with a previously refined “QTL-hotspot” region (~300Kb) for drought tolerance traits. Canopy conductance traits were mapped in CaLG03 (TR) and CaLG04 (eTR). iii) The refined “QTL-hotspot” region (Bin-Map) was further sub-divided into a “QTL-hotspot- a” and “QTL-hotspot- b” regions. While both “QTL-hotspot” sub-regions co-mapped with previous study , the phenotyping data at a lower level of plant organization gathered here led us to interpret that region ‘a’ (139.22Kb or 0.23 cM) could be a locus for branching and tissue/organ expansive processes while region ‘b’ (153.36Kb or 0.22 cM) could be interpreted as a locus for physiological processes related to biomass accumulation. iv) As marker density increased QTL number and size decreased (~ 29 cM to 0.22 cM); and LOD and PVE (%) increased for most of the QTLs. v) Most of plant vigour traits had alleles from high vigour parent ICC 4958 whereas in the case of canopy conductance traits (eTR and TR) the favourable alleles were contributed by the low vigour parent ICC 1882. vi) Plant vigour traits mapped mostly on CaLG04 whereas canopy conductance traits mapped on CaLG03, providing an opportunity to manipulate these loci to tailor recombinants having lower transpiration rate and high plant vigour desirable for water limited environments.
Phenotyping at different level of plant organization
The vigour traits (3DL, PL, SDW, PH, and 3D-LG) were tightly linked to plant water use traits. These traits were reported to be linked to crop biomass production and then crop yield [25, 26]. The co-localization study clearly demonstrated the close relationship between traits from the present study at a lower level of plant organization (eg. 3D-Leaf area, growth rate) and the agronomic traits (eg.shoot biomass, harvest index) studied previously by Varshney et al. . Canopy development traits had also a clear effect on crop production . Although phenotyping of traits at a lower level of plant organization is usually laborious and time-consuming process, it was facilitated by the use of a high throughput phenotyping platform (LeasyScan). Most of the plant vigour traits had high heritabilities, making them suitable for breeding applications. The high vigour parent ICC 4958 had higher biomass and water use (absolute T) than the low vigour parent ICC 1882. By contrast, the high vigour parent had lower transpiration rate (TR; g of water transpired per unit of leaf area) than low vigour parent. Hence, the cause for such response in water-use was the difference in leaf area (vigour/canopy development). The effect of such combination, having high vigour and lower TR would be then of high value to test across time and geographical scale using crop simulation analysis. Crop simulation modelling of water saving traits (eg. limited transpiration rate) has indeed shown a clear yield advantage under terminal drought stress conditions (Soybean-, Maize- and Sorghum-).
Co-localization of plant vigour traits and previously identified drought tolerance traits in different genetic maps
Early plant vigour is an important trait for water limited environments. It may contribute to shading of the soil surface, thereby reducing evaporation of water from the soil and leaving more water available for the crop [25, 29, 30]. In the present study, most of the plant vigour traits had several M-QTLs on CaLG04 and co-mapped with the earlier reported fine mapped “QTL hotspot” region [18, 20, 21] with QTLs for root traits. The alleles for these vigour traits were contributed by high vigour parent ICC 4958. Here is a first detailed study reporting the co-mapping of plant vigour traits with root and so-called drought tolerance traits. This is also a confirmation of the earlier observations that shoot dry weight and root length density QTL co-mapping in preliminary results . This result, therefore, suggests that the drought tolerance reported earlier to be associated with that QTL in the hotspot region (241 Low density SSR marker-; 1007-High density GBS markers- and Ultra-high density Bin maps-) would actually be conferred by plant vigour aspects. Such result was also predicted by a crop simulation study  that concluded that in the short duration environments where chickpea cultivation is now mostly cultivated, a high plant vigour associated with faster rooting would be necessary to reach the water available deep in the soil profile. Similarly, in recent pearl millet mapping studies [32, 33] it was reported that plant vigour traits also co-localized with agronomic traits related to terminal drought tolerance ; drought index of stover yield, grain yield, biomass yield and harvest index [35,36,37]. Similarly, another study in a high- resolution cross (HRC) population of pearl millet showed that plant vigour traits (3D-leaf area, plant growth rate, plant height) measured from LeasyScan co-localized with yield traits measured in the field under different water stress treatments (Tharanya et al-unpublished data. The present study suggests that high root length density obtained earlier  could be more easily proxied by vigour traits at the canopy level, which would then ease the phenotyping of that particular trait. Overall, plant vigour traits might lead to high biomass, which would then link to higher yield potential. Therefore, the genotypes that have alleles from ICC 4958 would be beneficial for water limited conditions.
Binmap QTL hotspot region
With the ultra-high density marker, the refined QTL-hotspot was sub-divided into two sub-regions “QTL-hotspot”-“a” & “QTL-hotspot”-“b”. Our interpretation, on the basis of the phenotyping at a lower level of plant organization done in the present study, is that these two regions could control two domains of physiological processes. “QTL-hotspot”-“a” region, which had QTL for traits related to vigour and growth rate (PH and VIG), could be interpreted as a region coding for branching and expansive processes. We interpret the possible effect on the branching from the two fairly opposite phenotypes of the parents of the population used here, i.e. highly branched ICC 1882 with low height versus less branched but taller ICC 4958. More work would be needed to decipher in more details the possible interaction between height and branching. The interpretation of the expansive processes comes from recent genetic work on regions controlling leaf expansion in maize , and where vigour could simply be consequences of differences in the expansive processes leading to larger organ sizes and quicker development. Interestingly, this region ‘a’ was earlier reported to harbour QTL for pod number per plant, 100-seed weight and plant height , although a finer analysis of the plant processes possibly involved was not done. It was particularly interesting to see that this region led to seed size differences, which then raises the question whether seed size is not itself controlled by expansive processes at the time of embryo development and seed formation. “QTL-hotspot”-“b” region could then simply be a locus controlling physiological processes involved in biomass accumulation, which was corroborated by the QTLs found here for 3DL, LAI and SDW, or for biomass traits SDW and RTR traits in Kale et al. .
Twelve candidate genes were reported from this fine mapped “QTL hotspot” region (see, ) stating that most of the genes were involved in abiotic stress tolerance. The same genes were also reported to be associated with plant growth and development related functions (e.g. genes of serine threonine-protein kinases, E3 ubiquitin ligases, Leucine-rich repeat extension (LRXs), Protein IQ domain and Vicilin 47 K and Cotyledon vascular pattern (CVP2) genes that were reported to be associated with drought stress adaptation by Kale et al.,  were also reported to be associated with plant growth and development related process [39,40,41,42,43,44,45,46,47,48]. These reports additionally suggest that earlier reported “QTL-hotspot” region to be associated more likely with vigour related traits.
Ideotyping of plant vigour and canopy conductance genomic regions
An ideal ideotype for water limited environment would be the one having higher plant vigour (the proxy for higher biomass and yield) potential with restriction of transpiration under high VPD conditions. These combinations would achieve higher water use efficiency, eventually soil moisture conservation, and then ultimately lead to crop production success. The plant vigour traits were mapped on CaLG04 and the canopy conductance (eg. TR) traits were present on CaLG03. These two genomic regions contributed more than 75% QTLs for plant water use (vigour and conductance) traits. Therefore, CaLG04 (plant vigour) and CaLG03 (canopy conductance) provide an opportunity to manipulate these loci to tailor recombinants having alleles with lower transpiration rate along with high plant vigour. This ideotype might be useful in enhancing the water stress adaptation in chickpea. Similar kind of ideotyping was recommended in pearl millet [32, 33]. Recent modelling reports on sorghum  showed that alteration of leaf area (plant vigour components) and transpiration rate increased grain yield under severe stress conditions. This study suggests that plant vigour and transpiration rate trait assessed in the current study might also have an effect on crop production success in specific target environments.
The present study has shown that a previously identified “QTL hotspot” region on LG04 of chickpea and harbouring QTL for root traits and so-called terminal drought tolerance in chickpea was a vigor locus, with favourable alleles from high vigour parent ICC 4958. Our phenotypic analysis at a lower level of plant organization led us to interpret that this locus may be divided into two sub-regions, one coding for expansive processes and one for biomass accumulation. Another genomic region on CaLG03 harboured QTL for canopy conductance traits (e.g. TR). Plant vigour and canopy conductance traits were somewhat negatively related but being mapped on different chromosome provides an opportunity to manipulate these loci to tailor recombinants having lower transpiration rate and high plant vigour which would be useful enhancing the drought adaptation in chickpea. In addition, potential genomic region on CaLG04 with simple vigour traits (e.g vigour score) could be used for breeding programs through marker assisted backcross (MAB) to devolep improved variety. Enrichment of the marker density reduced QTL size and increased in LOD and PVE% for all plant vigour and canopy conductance traits.
The genetic material was a set of 232 recombinant inbred lines from a population derived by single seed descent method from the cross between ICC 4958 and ICC 1882 and advanced to F10+ generation . Genotype ICC 4958 is a drought tolerant breeding line developed by Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, and Madhya Pradesh, India. It has a large root system, early vigour is early to reach 50% of flowering (608 cumulative degrees) and maturity (1650 cumulative degrees). The ICC 1882 landrace was collected in India and added to the ICRISAT’s genebank in 1973. It has a small root system, late vigour, is later to reach 50% of flowering (779 cumulative degrees) and maturity (1806 cumulative degrees) compared to ICC 4958 [8, 18, 49]. These two parental lines were contrasting for root traits and plant vigour i.e. were used for mapping population development. Additional detail account on parental lines and mapping population are provided in Varshney et al. .
Plant growth conditions
Phenotyping was performed from November to December 2014 & 2015 in the LeasyScan facility . Plants were sown during the post-rainy chickpea sowing window (November). Plants were grown in 27 cm diameter plastic pots filled initially with 9 kg of dry black soil (Vertisol) collected from ICRISAT farm. Each experimental unit in the LeasyScan platform was composed of 2 pots, each containing 4 healthy plants. These experimental units being of 65 × 40 cm, i.e. approximately 0.25 m2, the sowing density was 32 plant m− 2, which is equivalent to the sowing density in the field. In other words, phenotyping was done on a crop canopy that had close similarities with a field situation. Sowing was done with 6-8 seeds per pot and seedlings were thinned to maintain four homogeneous seedlings per pot at 12 days after sowing (DAS). Fertilizers were provided with single super phosphate (SSP) as basal dose at the rate of 0.3 g/kg of soil. The experimental design was an Alpha lattice with 4 replications and 24 blocks of 10 genotypes in each replication to avoid geographical variations. Plants were maintained under well water conditions the throughout experiment. During the crop grown period, 11/35.8 °C minimum and maximum temperature and 17.2/93.2% relative humidity were observed.
Phenotypic traits evaluated
Sixteen phenotypic traits were measured and categorized into three groups: (i) Canopy traits (measured by LeasyScan) (ii) Transpiration traits (measured by gravimetric balance system) and (iii) Biomass traits.
i) Canopy traits
LeasyScan PlantEye® scanners measured canopy development related traits [3DLeaf area (3D-L), projected leaf area (PL) and plant height (PH)] on the hourly basis during crop growth periods. Using these traits, plant growth rate related traits [3D-Leaf area growth rate (3D-LG), projected leaf area growth rate (PLG), plant height growth rate (PHG)] were calculated. Plant growth rate (3DLG, PLG, PHG) was calculated based on the average difference in respective leaf area and plant height between consecutive days during the exponential growth phase. The leaf area index (LAI) was estimated as the projected leaf area PL divided by the area of the pots in the sector. Plant vigour score was estimated by visual eye basis, on a scale from 1 (low vigour) to 5 (high vigour) at 20 DAS after sowing, all four replications being scored by one person eye visual score. Similar protocal was reported in other crop species such as wheat  and maize . Residual (canopy structure) was calculated by using 3D-leaf area and projected leaf area.
ii) Transpiration traits
Transpiration (evapotranspiration (eT)) was measured by a gravimetric method (see ). The pots were watered abundantly and drained overnight to attain field capacity. An extra 20 pots without plants were also brought to field capacity and were there to evaluate soil evaporation. Following day, plants were manually weighed (Model FCB 24 K0.2B, KERN & Sohn GmbH, Baligen, Germany.). All four replications were weighed between 6 and 7 am (Initial weight; average VPD~ 0.8 kPa). Pots were weighed again late afternoon between 3 and 4 pm (final weight; average VPD ~ 3.76 kPa), following the same sequence of pot weighing as in the morning. Evapotranspiration was calculated by the difference between initial and final pot weight. Further, plant transpiration (T) was estimated by subtracting an estimate of soil evaporation (pot without plant soil evaporation). Briefly, it was assumed that soil evaporation in planted pot would be maximum with zero plant cover, and would be zero at a leaf area index of 2.
Therefore, the projected leaf area was used to infer a LAI. Briefly,
LAI = PL/area of the pots in the sector.
At the time of eT measurements and transpiration values were estimated from this correction. While this may have induced some error, we made the assumption the method would be correct for genotypic comparison and QTL analysis. Transpiration rate (TR) and evapotranspiration rate (eTR) were calculated by transpiration and evapotranspiration divided by 3D-leaf area and time .
iii) Biomass traits
At the end of the experiment (canopy covered maximum in the pot; 35 DAS), shoot samples were harvested and over dried at 65 °C for 48 h. Further, shoot dry weights (SDW) were weighed using gravimetric balance (KERN 3Kg) method. Specific leaf area (SLA) was estimated by leaf area divided by shoot dry weight. Specific leaf weight (SLW) was estimated by 1/SLA (inverse of SLA).
QTL analysis- single locus
QTL analysis was conducted independently using three genetic maps developed earlier [18, 20, 21] and phenotyping data generated in this study. QTL Cartographer version 2.5, composite interval mapping (CIM) method was employed . For ultra-high density bin markers, inclusive composite interval mapping-Additive mapping (ICIM-ADD) method was used for identification of QTLs using IciMapping software (v3.2; ). LOD threshold was set by using 1000 permutation and p value ≤0.05. Constructed linkage map was visualized using Mapchart 2.2  software. When the PVE (phenotypic variation explained) was above 10%, QTLs were considered major QTLs (M-QTLs) and PVE below 10% were minor QTLs.
Interactions QTL analysis-multi-loci
The QTL interactions influencing the traits were identified using Genotype Matrix Mapping software (GMM; v. 2.1; , http://www.kazusa.or.jp/GMM). Using GMM, two and three loci interactions were tested. GMM analysis showed interactions between loci and different linkage groups of plant vigour and canopy conductance related traits. The current study identified allelic interactions that contributed to either a positive (increase) or negative (decrease) effect on the phenotypic value of the trait. In most cases, single locus QTL identified using GMM analysis were similar to those identified with CIM analysis, even though two approaches use different algorithms. In the following text, symbols “AA”, “BB” and “stand for alleles originated from the high vigour parent (AA; ICC 4958) and low vigour parent (BB; ICC 1882)” and not distinguished from any parent (−), respectively.
To find the phenotypic variations and their significance in the population, ANOVA was performed for all observed parameters individually using GENSTAT 14.0 (VSN International Ltd., Hemel Hempstead, UK). Similarly, to find the phenotypic variations and their significance in parental lines were analyzed with statistical program package CoStat version 6.204 (Cohort Software, Monterey, CA, USA). One-way ANOVA was carried out to test for genotypic difference between the genotypes. Means were compared using Tukey-Kramer test and Least Significant Difference (at P ≤ 0.05). Normal histograms with frequency distribution analysis for phenotypic traits were done using SPSS 16 desktop version (IBM, SPSS Statistical software). Principal component analysis (PCA) was used to visualize the relationships between traits in a multidimensional space using R software (version 2.11.1). To find the trait correlation of all phenotypic traits, simple Pearson correlation was performed using R software (version 2.11.1). For QTL and PCA analysis, Best Linear Unbiased Predictors (BLUPs) data were estimated by using GENSTAT 14.0 were used. The clustering analysis was performed by PCA loadings using R software (version 2.11.1). Genotypic and residuals mean square components were obtained from ANOVA through GENSTAT 14.0, which was used to calculate the broad sense heritability (h2). The broad-sense heritability (h2) was calculated as h2 = σ 2 G/ (σ 2 G + σ 2 E) [31, 32], where σ 2 G is the genetic variance and σ 2 E is the error variance.
Analysis of variance
Best linear unbiased predictions
Composite interval mapping
Days after sowing
Genotype by sequencing
Genotype matrix mapping
- h2 :
Inclusive composite interval mapping-Additive mapping
Logarithm of odds
Least significant difference
Principal component analysis
Phenotypic variation explained
Quantitative trait loci
Recombinant inbred lines
Single nucleotide polymorphisms
Simple sequence repeats
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The authors acknowledge Dr. Grégoire Hummel and Dr. Uladzimir Zhokavets from Phenospex for co-designing the LeasyScan platform with Dr. Vincent Vadez. Thanks, Mr. P.V.D Maheswar Rao and Mr. M. Anjaiah for their help in planting, irrigation and crop management practices.
This research was financially supported by ICRISAT for the capital investment in the LeasyScan facility and Blue Sky Research Project on “Developing crops with high productivity at high temperatures”. Additional funds were provided by CGIAR Research Program (CRP) on Grain Legumes.
Availability of data and materials
All data generated or analyzed during this study are included in the supplementary files as well as in the manuscript.
Vincent Vadez is working as Principal Scientist and Theme Leader – System Analysis for Climate Smart Agriculture (for more details see www.gems.icrisat.org) at ICRISAT in India, and now a Principal Scientist at IRD (Institut de Recherche pour le Developpement), Montpellier, France. He focuses mainly on phenotyping for drought adaptive traits in different legume and cereal crops. In addition, he has expertise in crop modelling of various drought adaptive traits and their possible production benefits in SAT agro-ecologies. He has collaborators across disciples from various international institutes.
Kaliamoorthy Sivasakthi is a Research Scholar pursuing his PhD in Crop Physiology Laboratory at ICRISAT in India. His Ph.D. work mainly focuses on Contribution of water saving traits for drought adaptation in chickpea (Cicer arietinum L.) through Physiological, Molecular and Genetics approaches.
Mahendar Thudi is currently working as Senior Scientist (Chickpea Genomics) at ICRISAT in India and leading the genomics and molecular breeding activities of chickpea. He developed large genetic and genomic resources in chickpea. Played a significant role in the generation of genome sequence of chickpea and re-sequencing of ~ 500 chickpea genotypes.
Murugesan Tharanya is a Research Scholar pursuing her PhD in Crop Physiology Laboratory at ICRISAT in India. Her Ph.D. work focuses mainly on “Contribution of water saving traits for drought adaptation in Pearl Millet (Pennisetum glaucum (L.) R. Br.) through Physiological, Molecular and Genetics approaches”.
Sandip M Kale is currently working as Visiting Scientist at Center of Excellence in Genomics at ICRISAT in India.
Jana Kholova` is currently working as Senior Scientist in System Analysis for Climate Smart Agriculture (SACSA) team at ICRISAT in India. She is mainly focuses on phenotyping for drought adaptive traits in semi-arid crops (Sorghum, Pearl millet, Chickpea etc.). In addition, she is expertise in APSIM crop modelling for various drought adaptive traits and their possible productions benefits in SAT agro-ecologies.
Mahamat Hissene Halime is a Research Fellow and completed her PhD in Crop Physiology Laboratory at ICRISAT in India.
Deepa Jaganathan is Research scholar and completed her PhD in Center of Excellence in Genomics at ICRISAT in India.
Rekha Baddum is working as Scientific Officer in Crop Physiology Laboratory at ICRISAT in India.
Thiyagarajan Thirunalasundari is working as Professor and Head, Department of Industrial Biotechnology, Bharathidasan University at Tiruchirappalli in India.
Pooran M. Gaur is Principal Scientist (Chickpea Breeding) at ICRISAT in India. He has extensive expertise on chickpea mapping population development and marker assisted breeding.
Rajeev K Varshney is Research Program Director – Genetic Gains at ICRISAT in India. Heis internationally recognized for his contribution in genome sequencing of pigeonpea, chickpea, peanut, pearl millet, sesame, mungbean and azuki bean and development of molecular breeding products.
Ethics approval and consent to participate
Not applicable – Data were generated from our own trials. The genetic material that was tested was readily available at ICRISAT and did not required us any kind of permit or request.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Cluster dendrogram analysis for collected phenotypic traits. Cluster analysis performed by major principal components using R-package. Two clusters (1 and 2) were shown on plant vigour traits and canopy conductance traits. (PPTX 55 kb)
Frequency distribution of plant vigour and canopy conductance related traits. Frequency distribution of plant vigour (A, B, C & D) and canopy conductance (E, F, G & H) related traits in chickpea mapping population (ICC 4958 x ICC 1882) showing normal distribution. A, B, C & D represent the plant vigour, plant height, 3D-leaf area and shoot dry weight (Plant vigour related traits) and E, F, G & H represent transpiration rate, evapotranspiration rate, transpiration and evapotranspiration (Canopy conductance related traits). P1 and P2 represent the ICC 4958 and ICC 1882). (PPTX 220 kb)
Growth dynamics of canopy development in contrasting parental lines. Growth dynamics of A) 3-leaf area and B) plant height in contrasting parental lines [High vigour parent (ICC 4958) and low vigour parent (ICC 1882) at vegetative stage calculated on the basis of thermal time (228-806 degree days for A and 114-806 degree days for B). (PPTX 181 kb)
Trait correlation analysis for the plant vigour and canopy conductance related traits. All the traits were evaluated under high throughput plant phenotyping platform (LeasyScan). (XLSX 10 kb)
Graphical representation of principal component analysis (PCA) for plant vigour and canopy conductance related traits. The plant vigour and canopy conductance traits vectors are represented by red arrows. The numbers represent recombinant inbred lines numbers (RIL numbers) and its position represents the particular trait loadings with respect to PC1 and PC2. BLUPs data across years were used for PCA analysis. (PPTX 117 kb)
Details on principal component analysis (PCA) for plant vigour and canopy conductance traits. (XLSX 10 kb)
Summary of QTLs for plant vigour and canopy conductance related traits using ultra-high density map. The QTLs were identified using ICIM (QTL IciMapping) software on ICC 4958 × ICC 1882 derived mapping population. (XLSX 12 kb)
Selected E-QTL interactions for plant vigour and canopy conductance traits. The E-QTLs were identified using genotype matrix mapping (GMM) software on ICC 4958 × ICC 1882 derived mapping population. (XLSX 12 kb)
Summary of E-QTL interactions for plant vigour and canopy conductance traits. The E-QTLs were identified using genotype matrix mapping (GMM) software on ICC 4958 × ICC 1882 derived mapping population. (XLSX 24 kb)
Summary of QTLs for plant vigour and canopy conductance related traits using low density marker. The QTLs were identified using QTL Cartographer software on ICC 4958 × ICC 1882 derived mapping population. (XLSX 17 kb)
Summary of QTLs for plant vigour and canopy conductance related traits using high density marker. The QTLs were identified using QTL Cartographer software on ICC 4958 × ICC 1882 derived mapping population. (XLSX 20 kb)
Genetic map of chickpea RIL population derived from ICC 4958 x ICC 1882. The genetic map represents marker position and corresponding marker name in linkage group. Genetic distances (cM) were shown on the left and markers are shown on the right side of the bars. The map was constructed using Map chart software. The Q represents QTL and R3 represents the population name. Year of mapping was represented by the symbols: # (2014), + (2015) and $ (across the year). A represent CaLG04 with identified QTLs position and its corresponding marker. The markers of the QTLs regions within the hotspot (most plant vigour related traits) were represented in red and outside the hotspot were represented in pink (QR3R-3D/PLA # + $), green (QR3-eTR+ $) and brown (QR3R-3D/PLA $). B) Map represents CaLG07 with QTLs for transpiration rate (QR3-TR) (markers highlighted in red colour) and residuals from 3D and projected leaf area (QR3R-3D/PLA) (markers highlighted in pink colour). C) Map represents CaLG06 with QTLs for residuals from 3D and projected leaf area (QR3R-3D/PLA) (markers highlighted in red colour). D) Map represents CaLG08 with QTLs for transpiration (QR3-T) (markers highlighted in red colour). (ZIP 283 kb)
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Sivasakthi, K., Thudi, M., Tharanya, M. et al. Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome. BMC Plant Biol 18, 29 (2018). https://doi.org/10.1186/s12870-018-1245-1
- Plant vigour
- Transpiration rate
- Quantitative trait loci (QTL)
- Drought stress