The cytosolic thiouridylase CTU2 of Arabidopsis thalianais essential for posttranscriptional thiolation of tRNAs and influences root development
© Philipp et al.; licensee BioMed Central Ltd. 2014
Received: 18 October 2013
Accepted: 22 April 2014
Published: 28 April 2014
A large number of post-transcriptional modifications of transfer RNAs (tRNAs) have been described in prokaryotes and eukaryotes. They are known to influence their stability, turnover, and chemical/physical properties. A specific subset of tRNAs contains a thiolated uridine residue at the wobble position to improve the codon-anticodon interaction and translational accuracy. The proteins involved in tRNA thiolation are reminiscent of prokaryotic sulfur transfer reactions and of the ubiquitylation process in eukaryotes. In plants, some of the proteins involved in this process have been identified and show a high degree of homology to their non-plant equivalents. For other proteins, the identification of the plant homologs is much less clear, due to the low conservation in protein sequence.
This manuscript describes the identification of CTU2, the second CYTOPLASMIC THIOURIDYLASE protein of Arabidopsis thaliana. CTU2 is essential for tRNA thiolation and interacts with ROL5, the previously identified CTU1 homolog of Arabidopsis. CTU2 is ubiquitously expressed, yet its activity seems to be particularly important in root tissue. A ctu2 knock-out mutant shows an alteration in root development.
The analysis of CTU2 adds a new component to the so far characterized protein network involved in tRNA thiolation in Arabidopsis. CTU2 is essential for tRNA thiolation as a ctu2 mutant fails to perform this tRNA modification. The identified Arabidopsis CTU2 is the first CTU2-type protein from plants to be experimentally verified, which is important considering the limited conservation of these proteins between plant and non-plant species. Based on the Arabidopsis protein sequence, CTU2-type proteins of other plant species can now be readily identified.
The accuracy of the translational machinery depends on the fidelity of codon recognition by the anticodons of transfer RNAs (tRNAs). tRNAs are short RNA molecules of 70–80 nucleotides that form defined secondary structures. Over one hundred different modifications of RNAs have been described, which are likely to influence their chemistry, metabolism, and stability . In tRNAs, such modifications can influence the codon-anticodon complex formation. The uridine at the wobble base (U34) of tRNAs for Lys, Glu, and Gln is universally modified to 5-methyl-2-thiouridine derivatives which enhance codon reading accuracy . The process of U34 thiolation has been well studied in a variety of organisms and involves a number of proteins that activate and eventually transfer the sulfur onto the U34. In yeast, the E1 ligase-like protein Uba4p (MOCS3 in humans) activates and thiolates Urm1p (Urm1 in humans), a ubiquitin-related modifier (URM) protein, resulting in a thiocarboxylate at the C-terminal glycine. The sulfur is then further transferred via the activity of two CYTOPLASMIC THIOURIDYLASE proteins (CTUs), Ncs6p and Ncs2p (Ctu1 and Ctu2, respectively, in humans) to the uridine residue of the target tRNAs [3–6]). The two CTU proteins interact with each other and share common motifs, but only CTU1 is able to bind tRNAs. The ATP-binding motif PP-loop in these proteins suggests that the sulfur transfer is an energy-consuming reaction .
This process is reminiscent of the sulfur transfer reaction in prokaryotes  but also shares similarities with the eukaryotic ubiquitylation system. In yeast and human cell lines, the URM proteins have also been shown to be involved in protein conjugation, particularly under oxidative stress conditions. The biological significance of this process, however, remains to be determined [6, 9]. The apparent conservation of the machinery involved in sulfur transfer and protein conjugation suggests an evolutionary relationship between these systems . An additional, interesting aspect of loci involved in tRNA thiolation is that they frequently have been identified as modifiers of the TOR (Target of Rapamycin) network [11, 12], a major controller of cell growth in eukaryotes . Since the regulation of translational activity is a target of the TOR pathway, it is quite possible that the lack of tRNA thiolation, which affects translational accuracy , has an impact on the TOR pathway via a feedback mechanism.
In plants, the investigation of the sulfur transfer reaction including thiolation of tRNAs is best investigated in Arabidopsis thaliana. The E1 ligase-like protein CNX5/SIR1 was shown to be important for the biosynthesis of molybdopterin . CNX5/SIR1 also transfers the sulfur to URM11 and URM12, the Arabidopsis homologs of URM proteins of yeast and humans [15, 16]. ROL5 represents the CTU1 homolog of Arabidopsis which binds to URM11 and URM12 and transfers the sulfur group from the C-terminal thiocarboxylate of the URM proteins to the tRNAs. ROL5, URM11 and URM12 can complement corresponding yeast mutants and mutations in the Arabidopsis ROL5, URM11, and URM12 genes interfere with tRNA thiolation. These data show that URM11, URM12, and ROL5 code for the orthologs of the respective yeast proteins and that the sulfur transfer process is conserved in Arabidopsis [15–17]. The CTU2 ortholog, however, has so far not been identified.
In Arabidopsis, mutations in cnx5/sir1 severely impact plant growth, reflecting the position of this protein in several sulfur-dependent processes such as molybdopterin biosynthesis and tRNA thiolation [14, 15]. In contrast, urm11 urm12 double and rol5 single mutants are mainly affected in root growth . rol5 was initially identified as a suppressor of the root hair cell wall formation mutant lrx1 (leucine-rich repeat extensin 1) [17, 18]. Correspondingly, mutating urm11 and urm12 also results in suppression of lrx1 . A likely explanation for this observation is the impact of defective tRNA thiolation on the TOR network in Arabidopsis. Interfering with TOR signaling by RNAi constructs targeting the TOR kinase or using specific inhibitors of the TOR protein activity have similar effects on root development as found for the rol5 or urm11 urm12 mutants [16, 17, 19–21].
This work experimentally identifies the second CYTOPLASMIC THIOURIDYLASE CTU2 of Arabidopsis. CTU2 is ubiquitously expressed in the plant and is essential for tRNA thiolation. Analysis of protein-protein interaction shows binding of CTU2 to ROL5, the CTU1 homolog of Arabidopsis. The analysis of a ctu2 knock-out mutant reveals an effect on root development, suggesting that the modification of tRNAs is particularly important for root developmental processes.
CTU2 is poorly conserved among different species
The Arabidopsis CTU2 homolog is necessary for tRNA thiolation
To analyze a possible effect of the ctu2-2 mutation on tRNA thiolation, tRNA was isolated from wild-type and ctu2-2 mutant seedlings and separated on a polyacrylamide gel supplemented with N-acryloylamino phenyl mercuric chloride (APM) which binds thiolated tRNAs, resulting in a higher molecular weight complex with slower migration in the gel. While tRNA isolated from the wild type showed the expected retarded band corresponding to thiolated tRNAs, these retarded tRNA species were absent in the ctu2-2 mutant (Figure 2C). Complementation of the ctu2-2 mutant with a wild-type CTU2 clone resulted in transgenic plants with reconstituted tRNA thiolation (Figure 2B, D). This suggests that the gene under investigation is indeed involved in tRNA thiolation.
CTU2 undergoes interaction with the CTU1-homolog ROL5
CTU2is ubiquitously expressed and influences root architecture
The rol5 mutant was initially identified as a suppressor of the root hair formation mutant lrx1 [17, 18]. To test whether ctu2-2 would have the same effect on lrx1, an lrx1 ctu2-2 double mutant was produced. While wild-type seedlings developed normal root hairs, those of lrx1 mutant seedlings showed the previously reported deformation phenotype [17, 18]. The lrx1 ctu2-2 double mutant seedlings, however, developed wild type-like root hairs (Figure 5B). The phenotypic penetrance in the different lines was very high and consistent. Hence, also the ctu2-2 mutation acts as a suppressor of lrx1.
tRNAs of bacteria and eukaryotes are altered by a variety of modifications . The uridine in the wobble position of tRNAs recognizing the split-codon boxes of Lys, Glu, and Gln codons are universally modified to 5-methyl-2-thiouridine derivatives (xm5s2U); the cytosolic tRNAs of eukaryotes containing 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) . The thiolation process requires a number of proteins that were identified in diverse organisms such as yeast, C. elegans, and humans. Among these proteins are an E1-like ligase, a ubiquitin-related modifier and two cytoplasmic thiouridylase (CTU) proteins [3–5, 7]. This work describes the experimental identification of the second CYTOPLASMIC THIOURIDYLASE, CTU2. While other plant homologs of the proteins involved could be readily identified based on amino acid sequence homology, [14–17], the level of homology of CTU2-type proteins across eukaryotes is rather low, making experimental evidence for the correct annotation of the Arabidopsis homolog necessary. The proteins of Arabidopsis and yeast or humans only share 20% identity, which is drastically lower than between CTU1-type proteins. These proteins in yeast and Arabidopsis (Ncs6p and ROL5, respectively) share 54% identical residues . This difference in conservation of the protein sequence suggests that CTU2-type proteins have undergone a stronger divergent evolution than CTU1-type proteins. A low sequence homology can indicate that initial substitutions of amino acids had to be compensated for by secondary mutations to maintain protein function, resulting in constant conformational characteristics despite low sequence homology [27, 28]. Alternatively, the function of CTU2-type proteins might tolerate more changes in the protein without affecting its intrinsic activity. Despite the limited sequence homology, the identified protein is highly likely to be CTU2 since it is essential for tRNA thiolation and interacts with the CTU1-type protein ROL5. The interaction of CTU1- and CTU2-type proteins has been well-established in several species [4, 5]. In in vitro experiments, the CTU1-CTU2 complex has been shown to be sufficient for tRNA thiolation, even though at a very low level . In vivo, however, the accumulation of biologically significant amounts of thiolated tRNAs requires the other protein components of this pathway.
CTU2 appears to be expressed in most if not all tissues. This is supported by the staining of transgenic plants containing an CTU2:GUS fusion construct and by gene expression profile analysis platforms such as Genevestigator  or At GenExpress Visualization Tool  that both indicate a moderate expression in all tissues. Despite the ubiquitous occurrence of tRNA thiolation, an obvious mutant phenotype of ctu2-2 is limited to root tissue. This might be related to the laboratory conditions under which the plants were grown, or the increased susceptibility of this tissue to changes in tRNA modification.
The impact of tRNA thiolation on developmental processes
Thiolation of the wobble position of specific tRNAs is important for effective translation by restricting base-pairing capability, preventing misreading of other near-cognate codons, and influencing the thermostability of the codon-anticodon interaction [29–31]. Yeast and worm mutants defective in this tRNA modification exhibit a temperature-sensitive growth phenotype . By contrast, increased CTU1 activity parallels increased cell growth, as observed in certain types of human cancer . Hence, the level of tRNA thiolation can have a substantial effect on cell viability.
In yeast, blocking tRNA thiolation by mutating Ncs6, Ncs2, or Urm1 causes a change in growth properties, resulting in the failure of yeast cells to induce pseudohyphal growth, an otherwise common response to nutrient-limited conditions in order to improve nutrient uptake efficiency [12, 33]. In plants, the tRNA thiolation mutants ctu2-2, urm11 urm12, and rol5 exhibit a reduction in lateral root density and are also affected in root hair development, exemplified by the suppression of the lrx1 root hair phenotype in lrx1 ctu2-2, lrx1 urm11 urm12, and lrx1 rol5 mutants ([16, 17], this work). Similar to pseudohyphae in yeast, the plant root system is important for nutrient uptake, indicating that changes in tRNA thiolation affect functionally related differentiation processes in very distinct organisms. Rather than being a coincident, this similarity might have a common basis in the TOR pathway, which senses nutrient availability and growth factors and adapts cell growth to prevailing conditions via influencing e.g. translation, mitochondrial activity, or cytoskeleton dynamics . The lack of tRNA thiolation affects translational activity, which is likely to feedback to the TOR network [11, 12], resulting in developmental alterations. Indeed, inhibiting TOR signaling by different means in Arabidopsis results in reduced root development and suppression of the lrx1 root hair phenotype as found for rol5, urm11 urm12, and ctu2-2 [17, 19–21, 35].
This work describes the experimental validation of the CTU2 protein of Arabidopsis thaliana, the second CYTOPLASMIC THIOURIDYLASE. The finding that protein-protein interaction between CTU1- and CTU2-type proteins of Arabidopsis takes place and that the CTU2 protein is essential for tRNA thiolation provides further support that the protein machinery involved in tRNA thiolation is well conserved among eukaryotes. The thiolation of tRNAs is not only physiologically important but is also connected to cell growth control. Hence, in future experiments it will be interesting to assess the potential of modifying plant growth properties and stress responses via modulating expression levels of genes involved in tRNA modification.
Plant material, growth conditions, and molecular markers
All Arabidopsis lines used are in the Columbia background. The lrx1-1 and rol5-1 alleles used in this study were previously described [17, 36]. The ctu2-1 and the ctu2-2 allele are the T-DNA insertion lines Salk_30197 and the Gabi-kat line 686B10-022973, respectively. Seed sterilization and plant growth was done as described .
For selection of mutant plants, the lrx1-1 allele was detected by a marker described in Diet et al. . The T-DNA insertion in ctu2-1 was detected with the gene-specific primer CTU2_F.1 TATGATGGATCAATGACTACTGAAG and the T-DNA specific primer SALK_Lb GCGTGGACCGCTTGCTGCAACT. For ctu2-2, the gene-specific primer CTU2_1050F CTCGTGTTTGTCTCCACCTGCTAA and the T-DNA specific primer Gabi_LB CCCATTTGGACGTGAATGTAGACAC were used. The CTU2 wild-type copy in ctu2-1 was amplified with the primers CTU2_F.1 TATGATGGATCAATGACTACTGAAG and CTU2_R.1 GTAGATGCTTCATTCAATTGCTC; the one of ctu2-2 was amplified with the primers CTU2_1050F CTCGTGTTTGTCTCCACCTGCTAA and CTU2_1777R CTGCCCTGCCCAGAATATGTGACG.
For complementation of the ctu2-2 allele, the genomic clone of CTU2 including 2.1 kb of promoter and 0.75 kb of terminator sequence was amplified from wild-type Columbia genomic DNA with the primers CTU2_promF TGGCATACCGACTTACTAGCTTG and CTU2_TermR TCTCACCATTCTAAAGCTTTGATC and cloned in pGEM-T easy (Promega). The insert of a correct clone was cut out with NotI and cloned into the plant transformation vector pBART  which is identical to pART27  but contains a basta- instead of kanamycin-resistance gene for plant selection. For the CTU2:GUS fusion construct, the CTU2 promoter was cloned into the plant transformation vector pGPTV-Bar .
For the yeast-two-hybrid experiment, a CTU2 cDNA was amplified from Columbia with the primers XbaI_At4g35910_1F TCTAGAATGGCTTGTAATTCCTCAGG and BamHI_At4g35910_1R GGATCCTTAGACAACCTCTTCATCGT and cloned into pGAD-HA cut with XbaI and BamHI. The ROL5 cDNA clone was amplified from a previously amplified cDNA clone  with the primers KpnI_At2g44270_1F GGTACCATGGAGGCCAAGAACAAGAAAGCAG and SmaI_At2g44270_1R CCCGGGTTAGAAATCCAGAGATCCACATTG and inserted in pLEXA_N (Dualsystems) cut with KpnI and SmaI.
Plant transformation and GUS staining
Plant transformation was performed as described , and transgenic seedlings were selected using 20 mg/L Basta and propagated to the next generation. GUS staining was performed in the T2 generation of five independent transgenic lines, in 50 mM Na-phosphate pH 7.0, 10 mM EDTA, 0.1% Triton X-100, and 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid during 4 hrs at 37°C.
tRNA extraction and analysis
Arabidopsis seedlings were grown vertically on half-strength MS plates for 14 days as described . Approximately 250 seedlings were used per extraction. The seedlings were grinded in liquid nitrogen and the material was extracted two times with 8 mL acidic phenol (Sigma), 0.8 mL chloroform and once with 4 mL acidic phenol, 0.4 mL chloroform. After extraction, tRNA was purified with AX100 columns from MACHEREY NAGEL following the manufacturer’s instructions. For analysis, the purified tRNA was separated on an acrylamide gel supplemented with N-acryloylamino phenyl mercuric chloride (APM) by the method adapted from Björk et al. .
Wild-type Columbia and ctu2-2 mutant seedlings were grown as descried  for 7 days in a vertical orientation. Entire seedlings were frozen in liquid nitrogen, grinded, and total RNA was extracted using the SV Total RNA Isolation System kit (Promega). The reverse transcription was conducted with 900 ng of total RNA using the i_script kit (Biorad). One tenth of the volume of the cDNA synthesis reaction was then used for RT-PCR using the primer pairs ACTIN2F AATGAGCTTCGTATTGCTCC and ACTIN2R GCACAGTGTGAGACACACC, and CTU2_rt_for CTCGTGTTTGTCTCCACCTGCTAA and CTU2_rt_rev TAGACAACCTCTTCATCGTCCAAG. The ACTIN2 PCR was done with 25 cycles, the CTU2 PCRs with 34 cycles of amplification.
Root phenotype analysis
Phenotypic observations and GUS activity analysis were done with a Leica LZ M125 stereomicroscope. Data points of lateral root development were taken after 8 days of growth in a vertical orientation. A t-test was performed to assess statistical significance of differences in lateral root formation. For the root hair phenotype, over 30 seedlings of each line were analyzed. Phenotypic variation was very small, i.e. each genotype showed a highly consistent root hair phenotype.
Yeast strains and growth conditions
Transformation and growth of yeast for the yeast-two-hybrid experiment was done following manufacturer’s instructions (Dualsystems).
The accession numbers of the genes used in this study are as follows: CTU2: At4g35910; LRX1: At1g12040; ROL5: At2g44270.
MP and FJ have made substantial contributions to the acquisition of the data and have been involved in writing the manuscript. CR has designed the project, contributed to data acquisition and written the manuscript. All authors read and approved the final manuscript.
We are grateful to Anja Meury Bechtel for excellent technical assistance and to Dr. S. Leidel for providing APM. This work was supported by the Swiss National Science Foundation grants Nr. 122157 and 138472.
- Rozenski J, Crain PF, McCloskey JA: The RNA modification database: 1999 update. Nucleic Acids Res. 1999, 27 (1): 196-197. 10.1093/nar/27.1.196.PubMed CentralView ArticlePubMedGoogle Scholar
- Björk GR, Huang B, Persson OP, Byström AS: A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast. RNA. 2007, 13 (8): 1245-1255. 10.1261/rna.558707.PubMed CentralView ArticlePubMedGoogle Scholar
- Schlieker CD, Van der Veen AG, Damon JR, Spooner E, Ploegh HL: A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway. Proc Natl Acad Sci U S A. 2008, 105 (47): 18255-18260. 10.1073/pnas.0808756105.PubMed CentralView ArticlePubMedGoogle Scholar
- Leidel S, Pedrioli PG, Bucher T, Brost R, Costanzo M, Schmidt A, Aebersold R, Boone C, Hofmann K, Peter M: Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature. 2009, 458 (7235): 228-232. 10.1038/nature07643.View ArticlePubMedGoogle Scholar
- Noma A, Sakaguchi Y, Suzuki T: Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res. 2009, 37 (4): 1335-1352.PubMed CentralView ArticlePubMedGoogle Scholar
- Van der Veen AG, Schorpp K, Schlieker C, Buti L, Damon JR, Spooner E, Ploegh HL, Jentsch S: Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier. Proc Natl Acad Sci U S A. 2011, 108 (5): 1763-1770. 10.1073/pnas.1014402108.PubMed CentralView ArticlePubMedGoogle Scholar
- Dewez M, Bauer F, Dieu M, Raes M, Vandenhaute J, Hermand D: The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity. Proc Natl Acad Sci U S A. 2008, 105 (14): 5459-5464. 10.1073/pnas.0709404105.PubMed CentralView ArticlePubMedGoogle Scholar
- Miranda HV, Nembhard N, Su D, Hepowit N, Krause DJ, Pritz JR, Phillips C, Söll D, Maupin-Furlow JA: E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea. Proc Natl Acad Sci U S A. 2011, 108 (11): 4417-4422. 10.1073/pnas.1018151108.PubMed CentralView ArticlePubMedGoogle Scholar
- Goehring AS, Rivers DM, Sprague GF: Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell. 2003, 14 (11): 4329-4341. 10.1091/mbc.E03-02-0079.PubMed CentralView ArticlePubMedGoogle Scholar
- Petroski MD, Salvesen GS, Wolf DA: Urm1 couples sulfur transfer to ubiquitin-like protein function in oxidative stress. Proc Natl Acad Sci U S A. 2011, 108 (5): 1749-1750. 10.1073/pnas.1019043108.PubMed CentralView ArticlePubMedGoogle Scholar
- Chan TF, Carvalho J, Riles L, Zheng XFS: A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR). Proc Natl Acad Sci U S A. 2000, 97 (24): 13227-13232. 10.1073/pnas.240444197.PubMed CentralView ArticlePubMedGoogle Scholar
- Goehring AS, Rivers DM, Sprague GF: Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p. Eukaryot Cell. 2003, 2 (5): 930-936. 10.1128/EC.2.5.930-936.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Laplante M, Sabatini DM: mTOR signaling in growth control and disease. Cell. 2012, 149 (2): 274-293. 10.1016/j.cell.2012.03.017.PubMed CentralView ArticlePubMedGoogle Scholar
- Teschner J, Lachmann N, Schulze J, Geisler M, Selbach K, Santamaria-Araujo J, Balk J, Mendel RR, Bittner F: A novel role for Arabidopsis mitochondrial ABC transporter ATM3 in molybdenum cofactor biosynthesis. Plant Cell. 2010, 22 (2): 468-480. 10.1105/tpc.109.068478.PubMed CentralView ArticlePubMedGoogle Scholar
- Nakai Y, Harada A, Hashiguchi Y, Nakai M, Hayashi H: Arabidopsis molybdopterin biosynthesis protein CNX5 collaborates with the ubiquitin-like protein URM11 in the thio-modification of tRNA. J Biol Chem. 2012, 287 (36): 30874-30884. 10.1074/jbc.M112.350090.PubMed CentralView ArticlePubMedGoogle Scholar
- John F, Philipp M, Leiber RM, Errafi S, Ringli C: Ubiquitin-Related modifiers of Arabidopsis thaliana influence root development. PLoS One. 2014, 9 (1): 22-View ArticleGoogle Scholar
- Leiber RM, John F, Verhertbruggen Y, Diet A, Knox JP, Ringli C: The TOR pathway modulates the structure of cell walls in Arabidopsis. Plant Cell. 2010, 22 (6): 1898-1908. 10.1105/tpc.109.073007.PubMed CentralView ArticlePubMedGoogle Scholar
- Baumberger N, Ringli C, Keller B: The chimeric leucine-rich repeat/extensin cell wall protein LRX1 is required for root hair morphogenesis in Arabidopsis thaliana. Genes Dev. 2001, 15 (9): 1128-1139. 10.1101/gad.200201.PubMed CentralView ArticlePubMedGoogle Scholar
- Deprost D, Yao L, Sormani R, Moreau M, Leterreux G, Nicolai M, Bedu M, Robaglia C, Meyer C: The Arabidopsis TOR kinase links plant growth, yield, stress resistance and mRNA translation. EMBO Rep. 2007, 8 (9): 864-870. 10.1038/sj.embor.7401043.PubMed CentralView ArticlePubMedGoogle Scholar
- Ren MZ, Venglat P, Qiu SQ, Feng L, Cao YG, Wang E, Xiang DQ, Wang JH, Alexander D, Chalivendra S, David L, Autar M, Gopalan S, Raju D: Target of Rapamycin signaling regulates metabolism, growth, and life span in Arabidopsis. Plant Cell. 2012, 24 (12): 4850-4874. 10.1105/tpc.112.107144.PubMed CentralView ArticlePubMedGoogle Scholar
- Xiong Y, Sheen J: Rapamycin and glucose-target of rapamycin (TOR) protein signaling in plants. J Biol Chem. 2012, 287 (4): 2836-2842. 10.1074/jbc.M111.300749.PubMed CentralView ArticlePubMedGoogle Scholar
- Bork P, Koonin EV: A P-loop-like motif in a widespread ATP pyrophosphatase domain: implications for the evolution of sequence motifs and enzyme activity. Proteins. 1994, 20 (4): 347-355. 10.1002/prot.340200407.View ArticlePubMedGoogle Scholar
- Zimmermann P, Hirsch-Hoffmann M, Hennig L, Gruissem W: GENEVESTIGATOR: Arabidopsis microarray database and analysis toolbox. Plant Physiol. 2004, 136 (1): 2621-2632. 10.1104/pp.104.046367.PubMed CentralView ArticlePubMedGoogle Scholar
- Schmid M, Davison TS, Henz SR, Pape UJ, Demar M, Vingron M, Scholkopf B, Weigel D, Lohmann JU: A gene expression map of Arabidopsis thaliana development. Nat Genet. 2005, 37 (5): 501-506. 10.1038/ng1543.View ArticlePubMedGoogle Scholar
- Phizicky EM, Hopper AK: tRNA biology charges to the front. Genes Dev. 2010, 24 (17): 1832-1860. 10.1101/gad.1956510.PubMed CentralView ArticlePubMedGoogle Scholar
- Suzuki T: Biosynthesis and function of tRNA wobble modifications. Topics in Current Genetics. Edited by: Grosjean H. 2005, 23-69. vol. 12Google Scholar
- Flores TP, Orengo CA, Moss DS, Thornton JM: Comparison of conformational characteristics in structurally similar protein pairs. Protein Sci. 1993, 2 (11): 1811-1826. 10.1002/pro.5560021104.PubMed CentralView ArticlePubMedGoogle Scholar
- Williams SG, Lovell SC: The effect of sequence evolution on protein structural divergence. Mol Biol Evol. 2009, 26 (5): 1055-1065. 10.1093/molbev/msp020.View ArticlePubMedGoogle Scholar
- Kruger MK, Pedersen S, Hagervall TG, Sorensen MA: The modification of the wobble base of tRNA (Glu) modulates the translation rate of glutamic acid codons in vivo. J Mol Biol. 1998, 284 (3): 621-631. 10.1006/jmbi.1998.2196.View ArticlePubMedGoogle Scholar
- Yarian C, Townsend H, Czestkowski W, Sochacka E, Malkiewicz AJ, Guenther R, Miskiewicz A, Agris PF: Accurate translation of the genetic code depends on tRNA modified nucleosides. J Biol Chem. 2002, 277 (19): 16391-16395. 10.1074/jbc.M200253200.View ArticlePubMedGoogle Scholar
- Murphy FV, Ramakrishnan V, Malkiewicz A, Agris PF: The role of modifications in codon discrimination by tRNA (Lys) UUU. Nat Struct Mol Biol. 2004, 11 (12): 1186-1191. 10.1038/nsmb861.View ArticlePubMedGoogle Scholar
- Yousef GM, Borgono CA, Michael IP, Davidian C, Stephan C, Jung K, Diamandis EP: Molecular cloning of a new gene which is differentially expressed in breast and prostate cancers. Tumour Biol. 2004, 25 (3): 122-133. 10.1159/000079144.View ArticlePubMedGoogle Scholar
- Gimeno CJ, Ljungdahl PO, Styles CA, Fink GR: Unipolar cell divisions in the yeast Saccharomyces cerevisiae lead to filamentous growth-regulation by starvation and RAS. Cell. 1992, 68 (6): 1077-1090. 10.1016/0092-8674(92)90079-R.View ArticlePubMedGoogle Scholar
- Wullschleger S, Loewith R, Hall MN: TOR signaling in growth and metabolism. Cell. 2006, 127 (3): 5-19.Google Scholar
- Sormani R, Yao L, Menand B, Ennar N, Lecampion C, Meyer C, Robaglia C: Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility. BMC Plant Biol. 2007, 7: 26-10.1186/1471-2229-7-26.PubMed CentralView ArticlePubMedGoogle Scholar
- Diet A, Brunner S, Ringli C: The enl mutants enhance the lrx1 root hair mutant phenotype of Arabidopsis thaliana. Plant Cell Physiol. 2004, 45: 734-741. 10.1093/pcp/pch084.View ArticlePubMedGoogle Scholar
- Kuhn BM, Geisler M, Bigler L, Ringli C: Flavonols accumulate asymmetrically and affect auxin transport in Arabidopsis. Plant Physiol. 2011, 156 (2): 585-595. 10.1104/pp.111.175976.PubMed CentralView ArticlePubMedGoogle Scholar
- Stintzi A, Browse J: The Arabidopsis male-sterile mutant, opr3, lacks the 12-oxophytodienoic acid reductase required for jasmonate synthesis. Proc Natl Acad Sci U S A. 2000, 97 (19): 10625-10630. 10.1073/pnas.190264497.PubMed CentralView ArticlePubMedGoogle Scholar
- Gleave AP: A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol. 1992, 20 (6): 1203-1207. 10.1007/BF00028910.View ArticlePubMedGoogle Scholar
- Becker D, Kemper E, Schell J, Masterson R: New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol. 1992, 20 (6): 1195-1197. 10.1007/BF00028908.View ArticlePubMedGoogle Scholar
- Diet A, Link B, Seifert GJ, Schellenberg B, Wagner U, Pauly M, Reiter WD, Ringli C: The Arabidopsis root hair cell wall formation mutant lrx1 is suppressed by mutations in the RHM1 gene encoding a UDP-L-rhamnose synthase. Plant Cell. 2006, 18 (7): 1630-1641. 10.1105/tpc.105.038653.PubMed CentralView ArticlePubMedGoogle Scholar
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