Figure 3

CTU2 interacts with ROL5. Protein-protein interaction between CTU2 and ROL5 (CTU1-type protein of Arabidopsis) was investigated by a yeast-two-hybrid experiment. In the presence of both proteins (left lane), yeast cells were able to grow under selective conditions and showed strong β-galactosidase activity, indicative of the interaction of the two proteins. No growing yeast cells were observed when transformation was done with the pGAD-CTU2 or pLEX-ROL5 constructs together with the empty second plasmid (pLEX and pGAD, respectively), indicating absence of self-activation function of either of the two proteins.