- Research article
- Open Access
Lr67 and Lr34rust resistance genes have much in common – they confer broad spectrum resistance to multiple pathogens in wheat
© Spielmeyer et al.; licensee BioMed Central Ltd. 2013
- Received: 7 February 2013
- Accepted: 27 June 2013
- Published: 2 July 2013
Adult plant rust resistance genes Lr67 and Lr34 confer race non-specific resistance to multiple fungal pathogens of wheat. Induced, susceptible mutants were characterised for both genes.
Three categories of Lr34 mutants were identified that were either partial susceptible, fully susceptible or hyper-susceptible to stripe rust and leaf rust. The likely impact of the mutational change on the predicted Lr34 protein correlated with differences in response to rust infection. Four independent Lr67 mutants were recovered that were susceptible to stripe rust, leaf rust and stem rust pathogens, including one possible hyper-susceptible Lr67 mutant.
Detailed study of Lr34 mutants revealed that subtle changes in resistance response to multiple pathogens were correlated with mutational changes in the predicted protein. Recovery of independent Lr67 mutants indicates that as for Lr34, a single gene at the Lr67 locus is likely to confer resistance to multiple pathogens. The infection phenotypes of Lr67 mutants closely resembled that of Lr34 mutants.
- Rust resistance
Many rust resistance genes are known in wheat but only a few provide durable, race non-specific resistance to multiple pathogens . The most widely deployed and best characterised of these genes, Lr34, confers durable, adult plant resistance (APR) to leaf rust caused by Puccinia triticina, stripe rust caused by Puccinia striiformis f.sp. tritici and powdery mildew caused by Blumeria graminis[2–5]. Lr34 resistance to each pathogen is partial while a second phenotype, so called leaf tip necrosis which involves necrosis of the tips of flag leaves, is also a characteristic of this gene . More recently, Lr67 was described which has similar characteristics to Lr34 in that it also confers leaf tip necrosis and provides partial, broad spectrum, adult plant resistance to leaf rust and stripe rust [7, 8]. Based on this phenotypic similarity Lr67 is predicted to also provide durable rust resistance, although this remains inconclusive as the gene has not yet been deployed over long periods of time and across a diverse range of germplasm and environments.
When Lr34 and Lr67 were transferred from landraces into the leaf rust susceptible wheat variety ‘Thatcher’ , leaf rust resistant backcross lines also acquired resistance to stem rust (P. graminis f.sp. tritici) at both seedling and adult plant stages [9, 10]. Seedling tests revealed that this stem rust resistance was probably race specific in contrast to the race non-specific responses of Lr34 and Lr67 to leaf rust and stripe rust. Further studies demonstrated that unlinked genetic loci interacted with Lr34 to enable the expression of stem rust resistance in Thatcher and Thatcher derivatives [11–14].
In this study, the wheat line Thatcher was used as a common genetic background for the identification and comparison of mutant phenotypes in the Lr34 and Lr67 genes, respectively. Previous analysis of six Lr34 mutants in the wheat line RL6058, a near-isogenic line of Thatcher with Lr34, confirmed that a single gene encoding a putative ATP-binding cassette transporter protein was responsible for Lr34 resistance to leaf rust, stripe rust and stem rust . We have isolated two additional RL6058 Lr34 mutants and, in conjunction with the six previously identified mutants, undertaken a detailed characterisation of their response to stripe rust and stem rust infection under field conditions and in response to leaf rust at the seedling stage when grown under controlled conditions. Four Thatcher Lr67 mutants were also identified which also showed a range of rust infection phenotypes closely resembling those of Lr34 mutants, further supporting a mechanistic similarity between these two genes.
Characterisation of Lr34 mutant phenotypes
Point mutations induced by sodium azide within genomic sequence of Lr34 gene in RL6058
Position of mutation (bp)
Effect on predicted protein
G to A
amino acid 889 change G to D
G to A
amino acid 1030 change G to E
G to A
amino acid 228 change E to K
C to T
premature stop codon after amino acid 567
G to A
splice site mutation at intron 3, premature stop
G to A
splice site mutation at intron 21, premature stop
G to A
splice site mutation at intron 1, premature stop
G to A
splice site mutation at intron 22, partial deletion
Similarly, mutant 4G, which showed partial susceptibility to stripe rust in the field, contained an intermediate level of chitin accumulation relative to the parental genotypes. Visual observations in the field did not distinguish mutant 2G from the susceptible Thatcher parent, however, a 30% reduction in chitin accumulation was observed which likely reflects the sensitivity of the chitin assay over empirical visual observations. In contrast, mutant 2F accumulated more chitin biomass than all other mutant lines and the susceptible Thatcher parent consistent with having an enhanced susceptibility to stripe rust infection. The remaining mutants accumulated chitin levels between the susceptible parent and the hyper-susceptible 2F mutant reflecting the increased variability in fungal infection between susceptible lines.
A loss of field resistance to stem rust was also observed in these mutant lines during two seasons in Australia. RL6058 was highly resistant (TR) while Thatcher was partially susceptible to stem rust (40–50 MRMS). Mutants were similar to Thatcher in their response to stem rust without apparent differences between mutants.
Mean infection site areas for leaf rust growth at 10 dpi on RL6058 (wt) and derived mutants 2G, 4C, 3E, 4E, 2B and 2F across three experiments
In summary, the leaf rust results from microscopic and macroscopic studies are in accordance with results obtained for stripe rust using field observations and fungal biomass quantification consistent with these mutants encoding heritable differences in their level of susceptibility to these pathogens. However, no detectable differences between mutants were observed in response to infection by stem rust.
Relating phenotypic differences to mutations in DNA sequence
Isolation and characterisation of Lr67 mutants
Comparison of Lr34 with Lr67
The resistance response of isogenic Lr34 and Lr67 lines to stripe rust and stem rust was similar, although in some field trials Lr34 resistance was marginally stronger. Previous studies also reported that field leaf rust resistance conferred by Lr34 was more effective than Lr67[7, 19]. The response to stripe rust infection revealed differences amongst both Lr34 and Lr67 mutants. Mutations within the Lr34 gene produced partial, full and hyper-susceptible mutants. A hyper-susceptible mutant was also recovered amongst Lr67 mutants and the quantification of fungal biomass suggested that partially susceptible mutants may have also been found but their phenotype was indistinguishable from other mutants under field conditions. An important difference between Lr34 and Lr67 was the lack of seedling resistance to leaf rust in RL6077 when grown under low temperature conditions. Under field conditions, both genes confer resistance to stripe rust, leaf rust and stem rust. Independent Lr34 mutants provided proof that a putative ABC transporter gene was responsible . Having been unable to separate the resistances to the different pathogens by mutation, we hypothesise that a single gene encoded by Lr67 is also responsible for resistance to the three rust pathogens in RL6077.
Different mutations within the Lr34 gene were associated with different levels of susceptibility to stripe rust and leaf rust. Mutants encoding single amino acid substitutions in the first or second nucleotide binding domain of the predicted protein showed only a partial loss of resistance suggesting that transport activity of the protein was compromised but not abolished, in contrast to fully susceptible mutants with predicted complete loss of protein function. Mutants that are expected to produce a severely truncated or non-functional protein were as susceptible as Thatcher, which encodes a predicted full length protein that varies by only two amino acid residues from Lr34 . These data imply that the protein encoded by the Thatcher allele may be non-functional and does not confer a basal level of resistance required for normal plant growth and development. The hypothesis requires further investigation given the level of variability in infection between Thatcher and fully susceptible lines across experiments. The hyper-susceptible 2F mutant encodes a Lr34 protein containing a deletion in the second transmembrane domain which may act as a ‘dominant negative’ mutant that interferes with other related transporters that contribute to basal immunity.
Analysis of Lr34 and Lr67 mutants confirmed previous reports that these genes confer resistance to multiple pathogens in a race non-specific manner. Given that sodium azide produces point mutations, the mutation analysis of Lr67 is consistent with a single gene conferring all resistance phenotypes associated with this locus. While the transfer of Lr34 or Lr67 into susceptible wheat backgrounds results in resistance to leaf rust and stripe rust, these genes do not confer effective stem rust resistance by themselves. For example, wheat lines containing Lr34 such as Chinese Spring, Terenzio and Sumai 3 are susceptible to stem rust. However, transfer of Lr34 into Thatcher results in the expression of effective stem rust resistance possibly through interaction of Lr34 with unlinked Thatcher genes. There is good evidence that the ‘enhancement effect’ of Lr34 on stem rust resistance is race specific suggesting that the mode of action is different to the race non-specific response conferred by this gene against leaf rust and stripe rust [11, 12, 14]. This study has confirmed that Lr67 is also associated with enhanced stem rust resistance in Thatcher. The donor line of Lr67, PI 250413, was stem rust susceptible demonstrating that Lr67 resembles the Lr34 phenotype in that it also interacts with unlinked genes in Thatcher for expression of stem rust resistance . It remains to be determined if the Lr67 response to stem rust is also race specific and if it requires the same Thatcher genes as Lr34 for resistance.
If these genes were combined, would they provide additional protection to three rust pathogens? A putative Lr34/Lr67 line (90RN2491) which was previously developed and screened for rust resistance is unlikely to carry Lr67 after this line was genotyped with a newly developed marker for Lr67 [19, Spielmeyer, unpublished]. New germplasm will need to be developed to evaluate the resistance response of the gene combination. Given the phenotypic similarities, it is possible that Lr34 and Lr67 encode proteins with similar function and lines carrying gene combinations that encode functionally redundant proteins may not out-perform single gene lines. The comparison of phenotypic differences amongst Lr67 mutants to causal mutations awaits the isolation of the gene.
Isolation and characterisation of mutants for Lr67 and Lr34 genes in a common genetic background enabled detailed studies of the resistance response to three rust pathogens. Mutants were assessed visually in the field, by quantifying chitin levels in field samples, macroscopic observations and microscopic analysis of seedlings grown under controlled conditions. The isolation of partial and hyper-susceptible mutants for both genes from within relatively small M2 populations is interesting and may provide insights how predicted proteins function and interact with components of basal resistance in future studies. We anticipate that marker development for Lr67 and as yet unidentified Thatcher- derived gene(s) that interact with Lr34 and Lr67 to confer stem rust resistance will add further value to both APR genes and provide greater rust protection in future cultivars.
Near-isogenic lines were developed by P. Dyck (AgCanada) by transferring Lr34 from the Chinese landrace PI 58548 and Lr67 from the Pakistani landrace PI 250413 by 6 backcross generations into the susceptible Thatcher background to produce RL6068 and RL6607, respectively.
Rust infection and phenotyping under controlled conditions
To screen for leaf rust resistance under low temperature conditions, one week old seedlings were inoculated with P. triticina pathotype 104–1,2,3,(6),(7),11 and incubated for 24 hrs at 20°C and high humidity before being moved to a growth cabinet set at a 10°C constant temperature and 16 hrs photoperiod.
Rust infection and phenotyping under field conditions
Lr34 mutants, parental lines and susceptible Thatcher control were grown in replicated field rows in a rust nursery at Cobbitty (Australia) in 2007, 2008, 2009 and 2012. Stripe rust Pucciniia striiformis f. sp. tritici pathotypes 134 E16 A + and 104 E137 A- +17, leaf rust P. triticina pathotypes 104–1,2,3,(6),(7),9,11; 104–1,2,3,(6),(7),11,13; 10–1,3,7,9,10,12; 76–3,5,9,10 + Lr37 and stem rust P. graminis f.sp. tritici pathotypes 98–1,2,3,5,6 and 34–1,2,7 + Sr38 were progressively released from tillering to late stem elongation stage. The modified Cobb scale was used to rate the disease responses with TR = trace resistant, MR = moderately resistant, MS = moderately susceptible and S = susceptible . Lr67 mutants, parental lines and controls were progeny tested in replicated field rows in 2010 at Cobbitty where they were scored for stripe rust and leaf rust resistance. In 2011, Lr67 mutants were evaluated in replicated rows at Cobbitty for stripe rust, leaf rust and stem rust resistance and again for stripe rust resistance (natural infection) in an experimental site at Canberra, Australia. In 2012, Lr67 mutants were pathotyped again at two sites in Cobbitty, Australia.
Isolation of mutants
Seed of RL6058 and RL6077 was pre-soaked in water for 12 hrs at 4°C before treating the grains in an oxygenated solution of 7 mM sodium azide at pH 3.0 for 2 hrs. The grains were rinsed and planted in the field. For each parental line approximately 1000 M2 single ear rows were scored for stripe rust resistance under field conditions. Seed was collected from putative mutants and resistant sibs and progeny plants of each were tested over several seasons for stripe rust, leaf rust and stem rust resistance in rust nurseries at Cobbitty and Canberra, Australia.
Microsopy and measurements of rust infection site areas
Wheat leaves (mostly 1st leaf) were inoculated with leaf rust and after 10 days prepared and stained with WGA-FITC as described in . Infection site areas which had produced infection hyphae were photographed under blue light using x10 magnification. Approx. 30–40 randomly selected infection sites were photographed for each line in one experiment and infection site areas calculated from photographic images using the AnalySIS Life Science Professional program (Olympus, Mt. Waverley, Australia). Kruskal-Wallis one way analysis of variance and Tukey test for the pairwise differences between means was used for statistical analysis of the data.
Wheat germ Agglutinin Chitin (WAC) assay
Flag leaves were harvested from 20–30 plants, at or near anthesis, grown in replicated field rows that were inoculated with stripe rust (see above). Tissue was cut into approx 2 cm long segments and mixed together before sub-sampling 1–2 grams of tissue for the chitin assay. Chitin quantification was undertaken as described in . Briefly, tissue segments were autoclaved in 1 M KOH and then neutralised in 50 mM Tris pH 7.0. The tissue was then homogenised by sonication and resuspended at a final concentration of 200 mg/ml. Replicated 20 mg samples were stained with WGA-FITC and tissues then washed three times with 50 mM Tris pH 7.0 buffer. Samples were finally resuspend in 100ul and fluorescence measured using a Wallac Victor 1420 multilabel counter (Perkin and Elmer Life Science) fluorometer with 485 nm adsorption and 535 nm emission wavelengths and a 1.0 second measurement time. A minimum of three replicates were undertaken for all samples.
We thank Ms. Melanie Soliveres for carrying out the chitin assay and Mr. Keshab Kandel for planting and maintaining field experiments.
- McIntosh RA, Wellings CR, Park RF: Wheat Rusts: An Atlas of Resistance genes. Australia: CSIRO Publications:1995,View ArticleGoogle Scholar
- Dyck PL: The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome. 1987, 29: 467-469. 10.1139/g87-081.View ArticleGoogle Scholar
- McIntosh RA: Close genetic linkage of genes conferring adult-plant resistance to leaf rust and stripe rust in wheat. Plant Path. 1992, 41: 523-527.View ArticleGoogle Scholar
- Morgounov A, Tufan HA, Sharma R, Akin B, Bagci A, Brown HJ, Kaya Y, Keser M, Payne TS, Sonder K, McIntosh R: Global incidence of wheat rusts and powdery mildew during 1969–2010 and durability of resistance of winter wheat variety Bezostaya 1. Eur J Plant Path. 2012, 132: 323-340. 10.1007/s10658-011-9879-y.View ArticleGoogle Scholar
- Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES: Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet. 2005, 111: 731-735. 10.1007/s00122-005-2058-9.PubMedView ArticleGoogle Scholar
- Singh RP: Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in wheat. Crop Sci. 1992, 32: 874-878. 10.2135/cropsci1992.0011183X003200040008x.View ArticleGoogle Scholar
- Hiebert CW, Thomas JB, McCallum BD, Humphreys DG, DePauw RM, Hayden MJ, Mago R, Schnippenkoetter W, Spielmeyer W: An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor Appl Genet. 2010, 121: 1083-1091. 10.1007/s00122-010-1373-y.PubMedView ArticleGoogle Scholar
- Herrera-Foessel SA, Lagudah ES, Huerta-Espino J, Hayden MJ, Bariana HS, Sing D, Sing RP: New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet. 2011, 122: 239-249. 10.1007/s00122-010-1439-x.PubMedView ArticleGoogle Scholar
- Dyck PL: Genetics of leaf rust reaction in three introductions of common wheat. Can J Genet Cytol. 1977, 19: 711-716.View ArticleGoogle Scholar
- Dyck PL, Samborski DJ: Adult-plant leaf rust resistance in PI 250413, an introduction of common wheat. Can J Plant Sci. 1979, 59: 329-332. 10.4141/cjps79-053.View ArticleGoogle Scholar
- Kerber ER, Aung T: Leaf rust resistance gene Lr34 associated with nonsuppression of stem rust resistance in the wheat cultivar Canthatch. Phytopathology. 1999, 89: 518-521. 10.1094/PHYTO.19220.127.116.118.PubMedView ArticleGoogle Scholar
- Vanegas CDG, Garvin DF, Kolmer JA: Genetics of stem rust resistance in the spring wheat cultivar Thatcher and the enhancement of stem rust resistance by Lr34. Euphytica. 2008, 159: 391-401. 10.1007/s10681-007-9541-0.View ArticleGoogle Scholar
- Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES: Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet. 2008, 116: 481-490. 10.1007/s00122-007-0684-0.PubMedView ArticleGoogle Scholar
- Hiebert CW, Fetch TG, Zegeye T, Thomas JB, Somers DJ, Humphreys DG, McCallum BD, Cloutier S, Singh D, Knott DR: Genetics and mapping of seedling resistance to Ug99 stem rust in Canadian wheat cultivars ‘Peace’ and ‘AC Cadillac’. Theor Appl Genet. 2010, 122: 143-149.PubMedView ArticleGoogle Scholar
- Krattinger SG, Lagudah ES, Spielmeyer W, Sing RP, Huerta-Espino J, McFadden H, Bossolini E, Selter L, Keller B: A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science. 2009, 323: 1360-1363. 10.1126/science.1166453.PubMedView ArticleGoogle Scholar
- Ayliffe M, Periyannan SK, Feechan A, Dry I, Schumann U, Wang M-B, Pryor A, Lagudah E: A simple method for comparing fungal biomass in infected plant tissues. Mol Plant Microbe Inter. in pressGoogle Scholar
- Rubiales D, Niks RE: Characterisation of Lr34, a major gene conferring non-hypersensitive resistance to wheat leaf rust. Plant Dis. 1995, 79: 1208-1212. 10.1094/PD-79-1208.View ArticleGoogle Scholar
- Ayliffe M, Devilla R, Mago R, White R, Talbot M, Pryor A, Leung H: Nonhost resistance of rice to rust pathogens. Mol Plant Microbe Interact. 2011, 24: 1143-1155. 10.1094/MPMI-04-11-0100.PubMedView ArticleGoogle Scholar
- Dyck PL, Kerber ER, Aung T: An interchromosomal reciprocal translocation in wheat involving leaf rust resistance gene Lr34. Genome. 1994, 37: 556-559. 10.1139/g94-079.PubMedView ArticleGoogle Scholar
- Peterson RF, Campbell AB, Hannah AE: A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Canad J Res Sect C. 1948, 26: 496-500.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.