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Table 1 Point mutations induced by sodium azide within genomic sequence of Lr34 gene in RL6058

From: Lr67 and Lr34rust resistance genes have much in common – they confer broad spectrum resistance to multiple pathogens in wheat

Mutant

mutation

Position of mutation (bp)

Effect on predicted protein

2G

G to A

7167

amino acid 889 change G to D

4C

G to A

9108

amino acid 1030 change G to E

4G

G to A

3444

amino acid 228 change E to K

2B

C to T

5317

premature stop codon after amino acid 567

3E

G to A

738

splice site mutation at intron 3, premature stop

3F

G to A

9974

splice site mutation at intron 21, premature stop

4E

G to A

276

splice site mutation at intron 1, premature stop

2F

G to A

10495

splice site mutation at intron 22, partial deletion