- Research article
- Open Access
Combined linkage and association mapping reveals candidates for Scmv1, a major locus involved in resistance to sugarcane mosaic virus (SCMV) in maize
BMC Plant Biology volume 13, Article number: 162 (2013)
Sugarcane mosaic virus (SCMV) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. Maize resistance to SCMV is associated with two dominant genes, Scmv1 and Scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. We combined both linkage and association mapping to identify positional candidate genes for Scmv1.
Scmv1 was fine-mapped in a segregating population derived from near-isogenic lines and further validated and fine-mapped using two recombinant inbred line populations. The combined results assigned the Scmv1 locus to a 59.21-kb interval, and candidate genes within this region were predicted based on the publicly available B73 sequence. None of three predicted genes that are possibly involved in the disease resistance response are similar to receptor-like resistance genes. Candidate gene–based association mapping was conducted using a panel of 94 inbred lines with variable resistance to SCMV. A presence/absence variation (PAV) in the Scmv1 region and two polymorphic sites around the Zmtrx-h gene were significantly associated with SCMV resistance.
Combined linkage and association mapping pinpoints Zmtrx-h as the most likely positional candidate gene for Scmv1. These results pave the way towards cloning of Scmv1 and facilitate marker-assisted selection for potyvirus resistance in maize.
Sugarcane mosaic virus is one of the most prevalent viral pathogens, causing significant global losses in maize grain and forage yields , especially in China and Germany [1, 2]. A recent report of SCMV in Poland and Central France revealed that the incidence of SCMV continues to increase [3, 4]. The diagnostic symptoms of infection with SCMV include stunting, chlorosis, and reduction in plant weight and grain yield [1, 5]. SCMV, formerly denoted as maize dwarf mosaic virus (MDMV) strain B, and belongs to the family Potyviridae. Other members of the family Potyviridae that also infect maize include MDMV, wheat streak mosaic virus (WSMV), Johnson grass mosaic virus (JGMV), sorghum mosaic virus (SrMV), and Zea mosaic virus (ZeMV) [6, 7]. Control of SCMV by agronomic and chemical means is ineffective owing to the non-persistent transmission mode of the virus by aphid vectors. Thus, deployment of resistant varieties is the most important way to prevent yield losses, which is based on screening and identification of resistant germplasm .
U.S. inbred line Pa405 shows complete resistance to MDMV and SCMV under both field and greenhouse conditions after artificial inoculation . In Europe, a collection of 122 early maturing maize inbred lines was screened for resistance against SCMV, resulting in the identification of three lines, D21, D32, and FAP1360A, that displayed complete resistance to SCMV . In China, many elite inbred lines display complete resistance when challenged with SCMV inoculum [2, 10]. Analysis of quantitative trait loci (QTL) in a cross between the resistant line D32 and susceptible line D145 revealed two major dominant genes on chromosomes 3 and 6, and three minor QTL on chromosomes 1, 5, and 10 [11, 12]. High-resolution mapping using progeny from the cross between FAP1360A (completely resistant to SCMV) and F7 (highly susceptible to SCMV) confirmed that Scmv1 (on the short arm of chromosome 6) and Scmv2 (near the centromere of chromosome 3) are crucial for maize resistance to SCMV . Similar mapping results have been reported in several independent experiments [8, 14, 15]. The Scmv1 locus has also been detected in a resistant Chinese line, Huangzao4, together with additional QTL on chromosomes 1, 3, 5, and 10 . In the progeny of Siyi×Mo17, two major genes were mapped on chromosome 3 (bin 3.04/05) and chromosome 6 (bin 6.00/01), respectively . By using an F9-derived immortalized RIL population obtained from the cross of Huangzao4×Mo17, a major QTL was detected between chromosome bins 6.00 and 6.01, accounting for 50.0% of the total phenotypic variation and decreasing the disease index by 27.9% . The loci on chromosomes 3 and 6 have also been confirmed in tropical maize germplasm from Brazil . With the aid of the whole-genome B73 sequence, the Scmv2 region was fine-mapped to a region of 0.28 cM, covering a physical distance of 1,342.6 kb. Four predicted genes possibly involved in virus movement are likely candidates for Scmv2. Existence of two closely linked resistance genes within the Scmv1 region has been postulated [18, 19]. Interestingly, several QTL that confer resistance to various viruses all map to the same region on chromosome 6 . It is currently uncertain, whether a single pleitropic Scmv1 gene confers resistance to different viruses, or distinct resistance genes are clustered in this region.
Even though diverse mapping populations have been used, all studies consistently identified the Scmv1 and Scmv2 genomic regions as being critical to confer resistance to SCMV. In this study, we first conducted the fine-mapping of Scmv1 using a large isogenic mapping population previously shown to segregate solely for Scmv1[13, 18, 19, 21, 22]. Second, we employed two RIL populations from elite parental lines with contrasting resistance to SCMV to confirm and further fine map the Scmv1 locus. Third, we identified a limited number of positional Scmv1 candidate genes based on the combined fine-mapping results. Finally, we conducted candidate gene-based association mapping to characterize the QTL region and candidate genes using a panel of 94 maize inbred lines with variable resistance to SCMV.
Fine-mapping of Scmv1 in a near-isogenic F2population
Of 177 unselected F2 plants, 50 exhibited typical symptoms of SCMV infection within 2–3 weeks after the first inoculation. The numbers of resistant and susceptible plants segregated in a 3:1 ratio (χ2 = 0.83 < χ20.01, 1), in agreement with inheritance of a single dominant gene. Using the 17 polymorphic simple sequence repeat (SSR) markers available from the Maize Genetics and Genomics Database (http://www.maizegdb.org/) (Additional file 1), the Scmv1 locus was mapped between umc1018 and umc1753, with a physical distance of 18.80 Mb according to the B73 physical map (Figure 1A). Owing to the lack of high-density markers, 28 recombinants between these two markers could not be resolved. Accordingly, we developed 11 new markers (Table 1) based on the public maize Bacterial Artificial Chromosome (BAC) sequences accessible in GenBank at the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov). We used these 11 new markers to resolve the 28 recombinants. Subsequent marker-phenotype analysis enabled us to progressively refine the Scmv1 region into a 710.97-kb interval between STS-5 and STS-15 (Figure 1B), with still two recombinants in between those two markers. Thereafter, the markers STS-5 and STS-15 were used to genotype 510 F2 susceptible plants, resulting in 13 new recombinants. Within the STS-5/STS-15 interval, single/low copy sequences were exploited to develop seven additional markers (Table 1). These markers were used to genotype 15 recombinants, which allowed us to delimit the Scmv1 locus into a 112.39-kb interval flanked by the two closest markers, R1-2 and STS-11 (Figures 1C and 1D). There were still six recombinants to the left and eight recombinants to the right side of the Scmv1 locus (Figure 1D). Comparison with the B73 physical map indicated that the two closest markers, R1-2 and the STS-11, reside on the adjacent BACs in GenBank: b0129G15 and b0598N23, respectively.
Validation of Scmv1in RILs
Given that the Scmv1 region could not be narrowed further using the near-isogenic F2 population, we used two RIL populations with parental lines contrasting in resistance to SCMV to confirm and fine-map the Scmv1 region. As both of the Scmv1 and Scmv2 loci are involved in maize resistance to SCMV, each RIL was investigated for its genotype at the Scmv2 locus using the co-segregating marker DJF004 developed by Ingvardsen et al. . Given that one of the Scmv1 flanking markers, STS-11, failed to reveal any polymorphism between parents of each of the two RIL populations, we searched for an alternative marker around STS-11 and finally developed a new marker, B4, which is located approximately 103.75-kb downstream of STS11. The B4 marker, together with the R1-2 marker, were then used to jointly genotype all RILs at the Scmv1 locus. For each RIL population, four sets of 30 RILs, which differed at either the Scmv1 or Scmv2 or both loci, were separately selected for phenotypic evaluation.
In the RIL population of Zheng58×Chang7-2, 28 of 30 RILs that share the same haplotype as Chang7-2 at both Scmv1 and Scmv2 loci (RR/RR) were highly resistant to SCMV. Of 30 RILs with the Chang7-2 haplotype at Scmv1, but the Zheng58 haplotype at Scmv2 (SS/RR), only one RIL was resistant to SCMV. Another two sets of 30 RILs sharing the same haplotype as Zheng58 at the Scmv1 locus (SS/SS and RR/SS) were all susceptible to SCMV. A similar result was observed in the RIL population of X178×HuangC, all 30 RILs sharing the same haplotype as X178 at both Scmv1 and Scmv2 loci (RR/RR) displayed high resistance to SCMV. Of 30 RILs with the X178 haplotype at Scmv1 and HuangC haplotype at Scmv2 (SS/RR), only four RILs were resistant to SCMV. Another two sets of 30 RILs which share the same haplotype as HuangC at the Scmv1 locus (SS/SS or RR/SS) were all susceptible to SCMV. These findings indicate that the presence of both Scmv1 and Scmv2 loci are prerequisite for SCMV resistance as described in previous reports . The significant difference (P < 0.01) in resistance percentages between RILs differing in haplotypes at both Scmv1 and Scmv2 loci suggested the presence of the same resistance loci in both RIL populations (Table 2).
Further fine-mapping of Scmv1in RILs
Given the validation result, the flanking markers B4 and R1-2 were used to screen for recombinants within the 215.47-kb Scmv1 interval. Eight and nine recombinants were detected in the RIL populations Zheng58×Chang7-2 and X178×HuangC, respectively. For the Scmv2 locus, a co-segregating marker DFJ004 was used to determine the genotype for each RIL. All recombinants were repeatedly investigated for their resistance to SCMV under both greenhouse and field conditions. On the other hand, through multiple comparisons of three resistant inbred lines, namely 1145, FAP1360A, and Huangzao4, and one susceptible B73 sequences within the B4/R1-2 interval, ten markers were developed to resolve the 17 recombinants.
Of the eight recombinants screened from the RIL population of Zheng58×Chang7-2, four RILs were as resistant to SCMV as the resistant parent Chang7-2, whereas another four RILs were as susceptible to SCMV as the susceptible parent Zheng58. The Scmv2 locus in three of the susceptible recombinants, Nos.70, 683 and 1045, was identical to that in the susceptible parent Zheng58, and thus they could not be used to define the Scmv1 locus. The four resistant RILs, Nos. 413, 966, 246, and 841, together with one susceptible RIL No. 1012 had the resistance allele as Chang7-2 at the Scmv2 locus and thus could be used to fine-map the Scmv1 locus. The four resistant RILs had the Chang7-2 donor region upstream of the marker B4, and one susceptible RIL No. 1012 had the Chang7-2 donor region downstream of the marker SNP4. These data support that the Scmv1 locus is located upstream of the marker B4 (Figure 2A). The mapped Scmv1 region in the Zheng58×Chang7-2 RIL population spanned the 112.39-kb Scmv1 region revealed by the near-isogenic F2 population and was thus of no value to further narrow down the Scmv1 locus.
Among the nine recombinants screened from the RIL population X178×HuangC, two displayed the same high resistance to SCMV as seen in the resistant parent X178, whereas another seven were as susceptible to SCMV as the parent HuangC. Three highly susceptible RILs, Nos. 1779, 1553, and 1746, carry the HuangC susceptibility allele at the Scmv2 locus. They were thus excluded from fine-mapping of the Scmv1 locus. Four RILs, Nos. 1900, 1876, 2041, and 2062, had the X178 resistance allele at the Scmv2 locus. However, they were all susceptible to SCMV. This finding indicates the absence of the X178 resistance allele at the Scmv1 locus for these four RILs. Among them, Nos. 1876 and 1900 RILs had the donor HuangC region downstream of the marker R1-2, and Nos. 2041 and 2062 RILs had the donor HuangC region upstream of the marker SNP3, suggesting the Scmv1 locus is confined by the markers R1-2 and SNP3. Two highly resistant RILs, Nos. 2009 and 1523, carried the X178 resistance allele at the Scmv2 locus and their Scmv1 regions were flanked by the markers 579P4 and B4. Thus, the Scmv1 locus can be narrowed down into a 59.21-kb region flanked by the markers 579P4 and SNP3 (Figure 2B), located within the 112.39-kb interval defined by the near-isogenic F2 population.
Predicting putative genes within and around the mapped Scmv1region
A 215.47-kb sequence flanked by the markers B4 and R1-2 was retrieved from http://www.maizesequence.org to identify candidate Scmv1 genes. There are still seven gaps left in this region, indicating some missing sequences. Transposable elements account for 55.27% of this region, of which 82.28% are retrotransposons, and 99.87% of the retrotransposons are long terminal repeat elements (data not show). We used Fgenesh software (http://linux1.softberry.com/berry.phtml, version 2.6) to predict ten putative genes from the masked sequence, including seven genes with putative functions, two hypothetical genes, and one gene without significant similarity to known genes (Figure 3C). No typical resistance (R) gene was found in the Scmv1 region. Analysis of possible functions for the seven predicted genes with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation indicated three of the genes were likely to confer resistance to SCMV (Table 3).
Two of the predicted genes are cycloartenol synthase1-like (CAS1-like) genes with high sequence similarity to genes from Arabidopsis thaliana. The first had the coverage of 76% and E-value of 1.00E-129, whereas the second had the coverage of 60% and E-value of 1.00E-74. These two CAS1-like genes have 74.18% similarity (204/275), with an E value of 2.00E-117, although they differ in the lengths of their amino-acid sequences. We designated the larger gene (403 residues) as CAS1-like-1, and the smaller one (360 residues) as CAS1-like-2. The other candidate gene, Zmtrx-h, encodes a putative 119-residue protein that is highly similar to thioredoxin (100% coverage; E = 4.00E-64), an oxidoreductase that acts on sulfur groups of donors, using disulfide as an acceptor.
Integration of the mapping results indicated that Scmv1 localizes to a 59.21-kb region flanked by 579P4 and SNP3 (Figure 3B). Of the five predicted genes located in this region, CAS1-like-2 and Zmtrx-h are more likely than the other three genes to confer resistance to SCMV.
Candidate gene–based association mapping of the Scmv1locus
To verify the candidate region and possibly refine the analysis by identifying quantitative trait nucleotide polymorphisms, we conducted an association mapping study using a panel of 94 inbred lines (Additional file 2), which were divided into six subgroups based on 70 SSR markers (Additional file 3). Multiple sequence alignment analysis identified 30 polymorphisms in six informative amplicons. Linkage disequilibrium (LD) analysis revealed lower r2 between these polymorphisms (Figure 4), with 91.03% of the r2 < 0.20. Two (single nucleotide polymorphism) SNP sites, S454 and S126, separately located on the 3′-end and intron of Zmtrx-h were significantly associated with SCMV resistance (P < 0.05; Table 4 and Additional file 4). Nonetheless, a low r2 value of 0.01 indicated two independent functional variants.
Given that 19 of the 94 maize inbred lines, including Mo17, could not be amplified by polymerase chain reaction (PCR) using any of the 17 primer pairs scattered throughout the Scmv1 region, we were able to identify a large PAV covering the Scmv1 region. This PAV was verified by Springer et al. , who reported a 2.6-Mb region on chromosome 6 including the Scmv1 region present in the B73 genome but not in Mo17. Strikingly, all of the 19 inbred lines were susceptible to SCMV, resulting in a strong association with SCMV resistance (P = 9.96E-4).
Precise phenotypic evaluation is crucial for marker-trait association analysis [24–27], especially for those traits with incomplete penetrance and phenotypic plasticity. Apart from being influenced by two major genes, resistance of maize to SCMV is also affected by genetic background and various environmental factors [13, 17, 18]. To obtain reliable phenotypic data, we developed a F2 mapping population from a near-isogenic pair where the resistance allele was fixed at the Scmv2 locus, and only Scmv1 was segregating . Furthermore, selection of only susceptible individuals from the F2 population for fine-mapping of the Scmv1 locus reduced the possibility of misclassification, as heterozygotes tend to be more plastic in their response to SCMV inoculation.
Advances in multiple genomic platforms and analytical methods allow fine-mapping and cloning of QTL responsible for important agronomic traits . In the current study, development of polymorphic markers in the 112.39-kb Scmv1 region was a challenge due to highly repetitive sequences. We thus sequenced the positive BAC clones from the 1145, FAP1360A, and Huangzao4 BAC libraries to mask the repetitive sequences at the Scmv1 locus, in order to reveal single/low-copy sequences for marker development. Generally, re-sequencing of more maize inbred lines  and the availability of additional publicly accessible sequence information (such as for Mo17: http://www.phytozome.net/maize.php; and Palomero: http://www.palomerotoluqueno.org/index.php) will facilitate the development of markers in targeted genome regions. During the process of fine-mapping, we found that the published order of BAC contigs (http://www.maizesequence.org) for the Scmv1 region is incorrect. The BAC-based B73 whole-genome physical map suggests that the adjacent BAC contigs #260 and #261 should be physically close to one another. However, the genetic distance is so large that none of the recombinants within the umc1018/umc1753 interval could be further resolved by the newly developed markers based on the contig #261. Only the markers developed from the contig #263 could resolve the recombinants. This indicates that contig #263 is located between contigs #260 and #261, and that the correct contig order must be #260-#263-#261-#262-#264 (Figure 3A). This correct contig order has been released (http://www.maizesequence.org).
Searching for recombinants with an unambiguous phenotype is crucial for fine-mapping. Thus, we used only highly susceptible individuals in the F2 mapping population for fine-mapping, which allowed us to fine-map the Scmv1 locus into a 112.39-kb region flanked by R1-2 and STS-11. From the fact that the Scmv1 locus has been repeatedly detected [8, 12, 14, 21], we inferred that Scmv1 is likely present in various resistant inbred lines. Based on this hypothesis, two RIL populations were used to fine-map the Scmv1 locus into a 59.21-kb region flanked by the markers 579P4 and SNP3.
The public B73 sequence suggested that two predicted genes, the CAS1-like homolog and Zmtrx-h gene, are the most likely candidates for Scmv1 owing to their potential roles in disease defense response [31, 32], although the potential influence of hypothetical proteins in the Scmv1 region on SCMV resistance cannot be excluded. Comparison with the complete CAS1 gene from Arabidopsis thaliana, which encodes 759 residues, suggests that the putative CAS1-like gene may be partial gene containing the TERPENE_SYNTHASES motif. CAS1 is involved in biosynthesis of steroids and non-steroidal secondary metabolites, such as campesterol and stigmasterol . Campesterol is the precursor of brassinosteroids, an important class of plant hormones that functions in cellular signal transduction, whereas the accumulation of stigmasterol stimulates an important plant metabolic process that occurs following bacterial infection of leaves . Given that phenotypic variance associated with changes in the Scmv1 region are influenced by the stage of plant development , it remains to be established whether the situation with Scmv1 closely resembles that of Hm2. a truncated duplicate of Hm1. Compared to Hm1, Hm2 preferentially functions in adult plants, conferring resistance against the leaf spot and ear mold disease caused by Cochliobolus carbonumrace 1[33, 34].
Thioredoxin is a master regulator of cellular redox status , with h-type thioredoxin reported to function in defense responses to viruses  and fungi . Given that the Zmtrx-h protein in the Scmv1 region lacks the conserved WC(G/P)PC motif, which is essential for redox protein activities, it seems unlikely that Zmtrx-h affects cellular redox status. Hence, additional investigation of the roles of the three candidate genes is required to uncover the mechanism of SCMV resistance.
Linkage and association analysis are two prevalent approaches to map genes or QTL. Both can be used in a complementary manner to dissect the genetic basis of traits of interest [38, 39], as well as to fine-map causative variants of targeted QTL . Six pairs of primers were used to test the association of specific stretches of sequence in the Scmv1 region with SCMV resistance. General linear model (GLM) analysis highlighted three polymorphisms associated with SCMV resistance. The significant association between the PAV and SCMV resistance validates the role of the Scmv1 region with disease resistance, whereas the association between two other SNPs and SCMV resistance provides statistical support for Zmtrx-h as the primary candidate for Scmv1. Moreover, these two SNP located in the intron and 3′-end of Zmtrx-h, respectively, seemingly having an influence on the gene’s expression. The CAS1-like-2 gene cannot yet be excluded, as there was no primer pair available in the vicinity of it. Therefore, a complementary functional test via transformation is still needed to identify which candidate gene, if any, defines Scmv1. These transgenic experiments are underway in our lab.
Broad-spectrum resistance (BSR), resistance against two or more types of pathogen species or the majority of races of the same pathogen species , is essential to improve the resistance of crops to various diseases. The quantitative resistance gene Lr34 cloned recently confers resistance to leaf rust, stripe rust, powdery mildew and various other diseases of wheat . The evidence for the presence of multiple disease resistance genes in a maize association population  revealed that quantitative BSR can be conferred by a single gene. The Scmv1 region has been reported to associate with resistance to other members of the Potyviridae family, including WSMV and MDMV [44, 45]. The near isogenic line F7RR/RR, which was used as a resistant parental line in this study, was also found to be resistant to MDMV and ZeMV . According to a model proposed by Kou , a single gene conferring BSR may function in either basal-resistance pathways, in overlapping pathways that confer race-specific resistances, or at sites of cross-talk between different defense pathways. Given that both candidate genes have putative roles in basal resistance, Scmv1 likely confers BSR, which would be advantageous in environments where plants are threatened by multiple pathogens. However, this speculation remains to be verified by artificial inoculation of transgenic plants and recombinants with the appropriate range of pathogens.
We have fine-mapped Scmv1 to a 59.21-kb region through integrating mapping data from different populations. This provides indispensable data for initiating molecular breeding to improve SCMV resistance in maize, a crop that is critical to world food security. We identified two candidates, of which the Zmtrx-h gene seems the most promising. Map-based cloning of Scmv1 and transgenic analyses will shed light on the molecular mechanism of this important plant-virus interaction.
Populations used for fine-mapping
The F7 near-isogenic lines, F7RR/RR, F7RR/SS, and F7SS/RR, derived from two early-maturing European maize inbred lines, FAP1360A (completely resistant to SCMV) and F7 (highly susceptible to SCMV), have been developed as described by Ingvardsen  (Additional file 5). A total of 2,200 F2 plants derived from the cross of F7RR/RR×F7RR/SS were collected as a mapping population for fine-mapping of Scmv1. Initially, a panel of 177 plants, randomly selected from the F2 mapping population, was used as an unselected mapping population to delimit the Scmv1 region. Each plant was mechanically inoculated with SCMV twice and scored at a weekly interval to evaluate resistance to SCMV. Additional markers in the Scmv1 region were developed to genotype the unselected F2 population. In parallel, the remaining 2,023 F2 plants were investigated for their resistance to SCMV, resulting in the identification of 510 plants with typical SCMV symptoms indistinguishable from their susceptible parent F7. These 510 susceptible F2 plants, together with 28 recombinants from the unselected F2 mapping population, formed the F2 sub-population for fine-mapping of Scmv1.
Validation of the Scmv1locus in two RIL populations
Two single-seed descent F6 RIL populations were employed to validate and further fine map the Scmv1 locus. One was developed from the cross between Zheng58 (susceptible to SCMV) and Chang7-2 (resistant to SCMV), a commercial hybrid (ZD958) that is currently widely cultivated in China. The other RIL population was derived from the cross between X178 (completely resistant to SCMV) and HuangC (highly susceptible to SCMV), another commercial hybrid (ND108) widely grown in China. A total of 2,206 F6 RILs (1,285 from Zheng58×Chang7-2 and 921 from X178×HangC) were planted in the experimental station at Shangzhuang, Beijing, during the summer of 2010. All RILs were genotyped using the markers R1-2 and B4 flanking the Scmv1 locus, as well as a co-segregating marker DJF004 at the Scmv2 locus . For each RIL population, four sets of 30 RILs, which differed at the Scmv1 or Scmv2 locus or both loci, were separately selected for phenotypic evaluation, whereas all recombinants between R1-2 and B4 were investigated for their SCMV resistance.
Seeds were sown in a soil/vermiculite mixture in 0.80-L pots maintained under greenhouse conditions. Virus inocula (Chinese isolate) were prepared from the SCMV-infected susceptible F7 plants. Young leaf tissue with typical mosaic symptoms was homogenized in five volumes of 0.01 M sodium phosphate buffer, pH 7.0. Carborundum was added to the sap to facilitate rub-inoculation. All leaves of each plant were rub-inoculated at the three- to four-leaf stage. One week after inoculation, all the symptomless plants were re-inoculated to avoid any escape. During sap preparation and mechanical inoculation, the inocula were put on ice to maintain low-temperature conditions.
For phenotypic evaluation of inbred lines and selected RILs, 12 kernels of each line were planted in four 0.80-L seeding pots. Whereas 10 plants were rub-inoculated with virus inoculum, two plants were not inoculated. These RILs were evaluated along with their parent lines as positive and negative controls for all tests. RILs with the same symptoms (P > 0.05, t-test) as the susceptible parent or the resistant parent were regarded as susceptible (S) or resistant (R). Three independent experiments were performed during September 2010 to July 2011 to ensure accurate phenotyping.
DNA extraction and molecular analyses
Immature leaf samples were harvested from each individual and immediately frozen in liquid nitrogen. DNA was extracted using the SDS method described by Murray and Thompson .
Apart from new markers developed from public BAC sequences, all SSR markers were retrieved from the Maize Genetics and Genomics Database (http://www.maizegdb.org) (Additional file 1) and synthesized by Invitrogen (Beijing, China). To design specific markers for fine-mapping, we first anchored the QTL in bin 6.01 to the B73 BAC-based whole-genome physical map (http://www.maizesequence.org/) using flanking SSR markers. We next extracted BACs, expressed sequence tags, and Indel polymorphic sequences (http://www.ncbi.nlm.nih.gov/; http://magi.plantgenomics.iastate.edu/browseMarkers.do) available in the targeted region to develop new polymorphic markers (SSR, STS, SNP, and CAPS). The retrieved sequences were analyzed with RepeatMasker (http://www.repeatmasker.org/cgi-bin/WEBRepeatMasker) followed by BLAST analysis against maize high-throughput genome sequence and genome sequence survey to remove redundant sequences . All primers for the single/low-copy sequences were designed using the software PRIMER 5.0 with the criteria that the primer length should be 20 nucleotides, with a 40–60% GC content, no secondary structure, and no consecutive tracts of the same nucleotide. The microsatellite search tool SSRHunter1.3  was used to mine SSR sequences of 4–6 tandem copies of >2 base pairs.
For PCR-mediated detection of SSR and STS markers, reactions were prepared in a 10-μL reaction volume containing 100 ng of genomic DNA, 1 μL of 10× PCR buffer, 0.2 mM of each dNTP, 0.2 μM of each forward and reverse primer, and 1 U of Taq polymerase. To detect SNP and CAPS markers, 50 μL reactions contained 300 ng of template DNA, 5 μL of 10× PCR buffer, 1 mM of each dNTP, 1 μM of each forward and reverse primer, and 5 U Taq polymerase (Beijing Transgen Biotech Co.LTD). All PCR reactions were performed using a PTC-200 Peltier Thermal Cycler (MJ Research Inc., Canada) with particular annealing temperature and elongation duration adapted to each reaction. The PCR products were subjected to electrophoresis using either 1% agarose gels (visualized using a gel imaging system from Bio-Rad Laboratories Inc., USA) or 6% polyacrylamide gels (amplification products were visualized by silver staining). SNP markers were sequenced at the Beijing Genomic Institute.
Analysis of candidate genes
To predict candidate genes, we retrieved the DNA sequence of the Scmv1 region from the B73 whole-genome sequence (http://www.maizesequence.org) for candidate gene prediction. The repetitive sequence in the Scmv1 region was analyzed by RepeatMasker version 3.3.0 (http://www.repeatmasker.org). We used the Fgenesh program (http://linux1.softberry.com/berry.phtml, version 2.6) to predict genes present in the single/low-copy Scmv1 region. For functional analysis, the deduced amino-acid sequence of each putative gene was compared against the “nr” database via BLASTP on National Center of Biotechnology Information website (http://blast.ncbi.nlm.nih.gov/). Detailed GO and KEGG annotation was applied to the gene sequences predicted by BLAST searches (http://www.geneontology.org/ and http://www.genome.jp/kegg/).
Association mapping of the Scmv1region
A total of 94 maize inbred lines were phenotyped in two distinct experiments (October 2007, May 2010) under greenhouse conditions using the same artificial inoculation procedure as RIL populations. The same 94 inbred lines were genotyped using a set of 70 SSR markers that were evenly distributed throughout the maize genome (Additional file 6). Based on the B73 sequence and using Primer 3 (http://frodo.wi.mit.edu/) or Premier 5.00 (PREMIER Biosoft International, USA), we designed 17 pairs of PCR primers (Additional file 7) flanking the three candidate genes. We used these primers to first screen for polymorphisms between F7, FAP1360A, Zheng58, Chang7-2, HuangC, and X178. Most primer pairs were discarded because of unreliable amplification. Six primer pairs with polymorphic products were used to amplify these 94 inbred lines to identify potential polymorphic sites. Of these six polymorphic amplicons, two and three are located in the vicinity of CAS1-like-1 and Zmtrx-h, respectively, whereas no primer pair around CAS1-like-2 was available. Nucleotide sequences alignments were done using the multiple sequence alignment software MUSCLE  and subsequently refined manually. Q was inferred using STRUCTURE 2.2 [51, 52] with 70 SSR markers. Five independent runs were performed setting the number of populations (k) from 1 to 10, burn in time and MCMC (Markov Chain Monte Carlo) replication number both to 500,000, and a model for admixture and correlated allele frequencies. The k value was determined by LnP(D) in STRUCTURE output and an ad hoc statistic Δk based on the rate of change in LnP(D) between successive k. Lines with membership probabilities ≥ 0.75 were assigned to corresponding clusters; lines with membership probabilities < 0.75 were assigned to a mixed group. Groups were further subdivided into subgroups using a similar methodology. The runs most consistent with breeder’s knowledge about pedigree were used to assign lines into clusters. The levels of LD between two sites were calculated using TASSEL2.0 . The associations between the extracted polymorphic sites with Minor Allele Frequency (MAF) ≥ 0.05 and SCMV resistance were carried out using GLM in TASSEL2.0.
Bacterial artificial chromosome
Kyoto encyclopedia of genes and genomes
Minor Allele Frequency
Johnson grass mosaic virus
General linear model
Polymerase chain reaction
Quantitative trait loci
Recombinant inbred line
Sugarcane mosaic virus
Single nucleotide polymorphism
Sorghum mosaic virus
Simple sequence repeat
Wheat streak mosaic virus
Zea mosaic virus.
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This study was financially supported by the NSFC (31371625), Ministry of Agriculture of China (2011ZX08009-003-001), National High-tech and Development Program of China (2012AA10A306 and 2012AA101104). We thank Dr. Xiaohong Yang at the China Agricultural University for critical guidance of association analysis in this study.
The authors declare that they have no competing interests.
LJ, QL, CRI and UKF performed mapping experiment in segregating population. BW and JL provided two RIL populations. YT, QL, carried out fine-mapping in these two RIL populations. YZ, RZ and YT completed the association mapping work. MX and TL supervised the research, designed the experiments and were involved in data analysis. YT wrote the manuscript draft, MX and TL edited and revised the manuscript. All authors read and approved the final manuscript.
Yongfu Tao, Lu Jiang, Qingqing Liu contributed equally to this work.
Electronic supplementary material
Additional files 2: The 94 maize inbred lines and their resistance performance to SCMVR means resistance to SCMV, while S means susceptible to SCMV.(DOCX 18 KB)
Additional files 3: Population structure of 94 maize inbred lines estimated using 70 SSRs. Population structure was assessed by STRUCTURE. Each individual is represented by a vertical bar, partitioned into colored segments with the length of each segment representing the proportion of the individual’s genome from k = 6 groups. For all classes, a given group is represented: red, REID; Blue, P; green, LAN; Yellow, TSPT; pink, Z330; turquoise, OTHER. (DOCX 86 KB)
Additional files 4: Associations between SCMV resistance and polymorphic sites in the Scmv1 genome region. a: P-value calculated using GLM. #: polymorphic sites associated with SCMV resistance. (DOCX 16 KB)
Additional files 7: Primers designed for association mapping. *: The PCR products amplified by these primers contained polymorphic sites that could be used for association mapping. (DOCX 15 KB)
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Tao, Y., Jiang, L., Liu, Q. et al. Combined linkage and association mapping reveals candidates for Scmv1, a major locus involved in resistance to sugarcane mosaic virus (SCMV) in maize. BMC Plant Biol 13, 162 (2013). https://doi.org/10.1186/1471-2229-13-162
- Association mapping