- Research article
- Open Access
Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase
BMC Plant Biology volume 11, Article number: 73 (2011)
Haspin kinases are mitotic kinases that are well-conserved from yeast to human. Human Haspin is a histone H3 Thr3 kinase that has important roles in chromosome cohesion during mitosis. Moreover, phosphorylation of histone H3 at Thr3 by Haspin in fission yeast, Xenopus, and human is required for accumulation of Aurora B on the centromere, and the subsequent activation of Aurora B kinase activity for accurate chromosome alignment and segregation. Although extensive analyses of Haspin have been carried out in yeast and animals, the function of Haspin in organogenesis remains unclear.
Here, we identified a Haspin kinase, designated AtHaspin, in Arabidopsis thaliana. The purified AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. Live imaging of AtHaspin-tdTomato and GFP-α-tubulin in BY-2 cells showed that AtHaspin-tdTomato localized on chromosomes during prometaphase and metaphase, and around the cell plate during cytokinesis. This localization of AtHaspin overlapped with that of phosphorylated Thr3 and Thr11 of histone H3 in BY-2 cells. AtHaspin-GFP driven by the native promoter was expressed in root meristems, shoot meristems, floral meristems, and throughout the whole embryo at stages of high cell division. Overexpression of a kinase domain mutant of AtHaspin decreased the size of the root meristem, which delayed root growth.
Our results indicated that the Haspin kinase is a histone H3 threonine kinase in A. thaliana. AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. The expression and dominant-negative analysis showed that AtHaspin may have a role in mitotic cell division during plant growth. Further analysis of coordinated mechanisms involving Haspin and Aurora kinases will shed new light on the regulation of chromosome segregation in cell division during plant growth and development.
The mitotic phase, which comprises mitosis and cytokinesis, is a fundamental process for faithful transmission of genetic information from one cell generation to the next. The main purpose of mitosis is to segregate sister chromatids into two daughter cells. The regulation of mitotic progression relies mainly on two post-translational mechanisms; protein phosphorylation and proteolysis. Cell division is regulated by mitotic kinases, such as the cyclin-dependent kinase 1 (CDK1), the Polo family, the NIMA (never in mitosis A), and the Aurora family, as well as kinases implicated in mitotic checkpoints, mitotic exit and cytokinesis .
Post-translational modifications of core histones play a crucial role in chromatin structure and gene expression . Although the N-terminal sequence and phosphorylations of histone H3 are highly conserved among eukaryotes, the distribution patterns of phosphorylated histone H3 on the chromosomes differ between animals and plants. In mammalian cells, H3S10ph begins to appear in pericentromeric regions from G2 phase, spreading along the chromosome periphery until metaphase, and then disappearing at late anaphase . The phosphorylation pattern of H3S28 is similar to that of H3S10ph during mitosis [4, 5]. Because the spatial and temporal patterns of H3S10ph and H3S28ph are consistent with chromosome condensation and decondensation, it is thought that H3S10ph and H3S28ph have a crucial role in chromosome condensation in animals. In contrast, H3S10ph and H3S28ph occur in the pericentromeric regions--not along the whole chromosome--from prophase to anaphase in plants [6–8]. These distribution patterns suggest that H3S10ph and H3S28ph play a crucial role in cohesion and segregation of sister chromatids . In plants, AtAUR3 (Arabidopsis thaliana Aurora kinase3) phosphorylates histone H3 at Ser10 and Ser28 in vitro [8, 10, 11]. Inhibition of Aurora kinase by Hesperadin treatment prevents H3S10ph and H3S28ph in tobacco BY-2 cells and H3S10ph in Arabidopsis suspension cells [8, 12]. Thus, Aurora kinases phosphorylate histone H3 at Ser10 and Ser28 in plants.
H3T3 and H3T11 are also phosphorylated, but their distribution patterns differ from those of H3S10ph and H3S28ph during mitosis. In mammalian cells, H3T3ph and H3T11ph occur preferentially at the centromere from prophase to anaphase [13, 14]. In contrast, H3T3ph and H3T11ph are distributed along the entire length of the chromosome in plants [15, 16]. Aurora kinases phosphorylate histone H3 at Ser10 and Ser28, but the kinase responsible for H3T3ph and H3T11ph is yet to be identified in plants.
Haspin (haploid germ cell-specific nuclear protein kinase) was first identified as a testis-specific gene in mice [17, 18]. Although Haspin mRNA levels were highest in the testis, lower levels of Haspin mRNA were detected in other organs, suggesting that expression of Haspin is not truly specific to haploid germ cells . Human Haspin could phosphorylate histone H3 at Thr3 and was involved in chromosome congression during mitosis . The centromeric localization of H3T3ph and the Haspin-knockdown phenotype in human cells indicated that Haspin is required for maintenance of centromeric cohesion during mitosis [20, 21]. Recently, three studies on Saccharomyces cerevisiae, Xenopus, and human revealed the novel cascade leading to the recruitment of mitotic kinases to the centromere [22–24]. In S. cerevisiae, Haspin interacts with cohesin, and the cohesin-associated Haspin phosphorylates histone H3 at Thr3 on the inner centromere . The phosphorylated H3T3 then binds the chromosomal passenger complex (CPC) containing Aurora B, thereby recruiting CPC to the inner centromere [22–24]. Thus, CPC functions in determining the correct kinetochore-microtubule attachment for accurate chromosome alignment and segregation, and this function is regulated via H3 phosphorylation on the inner centromere.
Analyses of Haspin were first carried out in yeast and animals, and although it is clear that this protein has roles in mitosis and cell division, the function of Haspin in organogenesis remains unclear. In this study, we identified A. thaliana Haspin, characterized its kinase activity, and determined its localization during mitosis. Expression of a kinase domain mutant of AtHaspin inhibited root growth, suggesting that Haspin is involved in cell division during mitosis.
Haspin candidate gene in Arabidopsis thaliana
Genes encoding Haspin homologs have been identified in a wide variety of eukaryotes including vertebrates, invertebrates, plants, and fungi, but not in prokaryotes and archaea  (Figure 1A). Except for Caenorhabditis elegans and S. cerevisiae, most organisms have one Haspin kinase gene. In a BLAST search of the A. thaliana genome, one Haspin candidate gene showed high similarity (BLAST score = 196, E-value = 3E-50) to human Haspin kinase in the kinase domain. The second hit gene showed lower similarity (BLAST score = 46.6, E-value = 4E-5). One Haspin candidate gene has been identified in some plant species, including ferns, mosses, and algae (Figure 1A). Although there were two putative genes identified in Glycine max and Medicago truncatula, the synteny analysis from Phytozome  suggested that these genes were duplicated. In the A. thaliana genome, the putative Haspin gene (At1g09450) is designated as AtHaspin (A. thaliana Haspin-related gene). The C-terminal regions of Haspin proteins have a conserved kinase domain  (Figure 1B). The amino acid sequence of AtHaspin cDNA showed 38% similarity with human Haspin in the kinase domain. Recently, the crystal structure of the kinase domain of human Haspin was solved [28, 29]. Although AtHaspin showed low similarity to human Haspin across the entire kinase domain, the residues that act as ATP and Mg2+ ion-binding sites were conserved between human and A. thaliana (Figure 1C). These data suggested that the mitotic kinase function of Haspin may be conserved in plants.
AtHaspin phosphorylates histone H3 at Thr3 and Thr11 in vitro
The human Haspin protein K511A, which contains a mutation of a single conserved lysine residue that is important for ATP binding, has no kinase activity . To examine whether purified GST-AtHaspin has kinase activity, an in vitro kinase assay was performed using purified GST-AtHaspin and GST-AtHaspin KD (kinase dead) (K309A) with or without ATP. Phosphorylated proteins were detected by ProQ Diamond Phosphoprotein Stain. As expected, GST-AtHaspin-KD was not autophosphorylated even in the presence of ATP (Figure 2A, second lane). However, GST-AtHaspin was autophosphorylated in the presence or absence of ATP (Figure 2A, first and third lanes). This result indicated that autophosphorylation of AtHaspin was not dependent on addition of ATP, and that this lysine residue is also required for autophosphorylation of AtHaspin. This result also suggested that GST-AtHaspin was autophosphorylated during production in Escherichia coli.
The only known substrate of Haspin is the Thr3 of histone H3 . To determine whether AtHaspin is a histone H3 Thr3 kinase, we carried out an in vitro kinase assay using purified GST-AtHaspin, a positive control (GST-AtAUR3), and plant histone H3 as the substrate. The positive control, GST-AtAUR3, is a histone H3 Ser10 and Ser28 kinase . GST-AtAUR3 phosphorylated histone H3 at Ser10 and Ser28, while GST-AtHaspin phosphorylated histone H3 at Thr3 in vitro. Surprisingly, GST-AtHaspin also phosphorylated histone H3 at Thr11 in vitro (Figure 2B).
To confirm the specificity of antibodies against H3T3ph and H3T11ph, an in vitro kinase assay was performed with purified GST-histone H3 tail proteins containing mutations at Thr3, Thr11, and at several residues adjacent to them (Arg2, Lys4, Ser10, and Gly12). Using anti-H3T3ph antibodies, bands were detected in the case of normal histone H3 and S10A, T11A, and G12A mutants, but not in the case of R2A, T3A, or K4A mutants (Figure 2C). Using anti-H3T11ph antibodies, bands were detected in the case of R2A, T3A, and K4A mutants, but not in the case of S10A, T11A, or G12A mutants (Figure 2C). GST-AtHaspin-KD (kinase domain mutant; K309A) had no kinase activity towards H3 at Thr3 and Thr11 in vitro. These results indicated that the AtHaspin kinase phosphorylates histone H3 at Thr3 and Thr11 in vitro.
Subcellular localization of AtHaspin in BY-2 cells
To analyze the subcellular localization of AtHaspin during cell division, we transformed Nicotiana tabacum cv. Bright Yellow-2 (tobacco BY-2) cultured cells with GFP-fused AtHaspin and observed tobacco BY-2 cells stably expressing AtHaspin-GFP with DNA stained by Hoechst 33342 (Figure 3A). During interphase, AtHaspin was mainly localized in the cytoplasm and at the nuclear periphery. After nuclear envelope breakdown (NEBD), AtHaspin invaded the nuclear region. During metaphase, fluorescent signals of AtHaspin-GFP were also observed on the chromosome (Figure 3A, arrowhead). After metaphase, AtHaspin-GFP was localized with the phragmoplast from its initial formation at the center of the equatorial plane to its expansion towards the cell periphery as the cell cycle progressed.
To analyze the relationship between AtHaspin and microtubules, we observed transgenic BY-2 cells expressing GFP-α-tubulin and inducibly expressing AtHaspin-tdTomato. After NEBD, AtHaspin-tdTomato immediately invaded the nucleus, while α-tubulin remained at the nuclear periphery. During prometaphase and metaphase, microtubules organized the mitotic spindle, while AtHaspin-tdTomato was widely distributed over the spindle. AtHaspin-tdTomato signals were observed on the chromosomes aligned at the equatorial plate (Figure 3B, arrowhead). During anaphase, AtHaspin-tdTomato localized with the sister chromatids, and during telophase, it colocalized with the phragmoplast. As the phragmoplast expanded toward the cell periphery, AtHaspin was moved toward the cell periphery. However, the movement of AtHaspin-tdTomato differed from that of phragmoplast (Figure 3C, Additional file 1). At the onset of cell division in higher plants, the pre-prophase band (PPB), which is a dense band of cortical microtubules, begins to form at the future-cell-division plane. Until NEBD, AtHaspin-tdTomato was localized at the cytoplasm but not at the PPB (Figure 3C; a, arrowhead), and then AtHaspin-tdTomato dispersed after NEBD (Figure 3C; b and c). As the cell plate expanded during telophase, the phragmoplast was depolymerized in the center of the equatorial plate and repolymerized along the edge of the growing cell plate. Although the phragmoplast expanded toward the cell periphery, AtHaspin-tdTomato remained around the cell plate until the end of telophase (Figure 3C; f). Considering the dynamics of AtHaspin-tdTomato, AtHaspin could not be directly involved in the regulation of the dynamics of microtubules during mitosis.
To reveal whether AtHaspin phosphorylates histone H3 at Thr3 and Thr11 in vivo, we performed indirect immunofluorescence using anti-H3T3ph, H3T11ph, and H3S28ph antibodies in BY-2 cells (Figures 4A and 4B). During interphase, no signals of H3T3ph, H3T11ph, and H3S28ph were observed. The H3T3ph signal was first detected on chromosomes in early prophase before NEBD, whereas the H3T11ph signal was first detected on chromosomes in late prophase after NEBD. During late prophase, H3T3ph signals were observed along the chromosome, while H3S28ph signals were observed at pericentromeric regions. During prometaphase, the H3T3ph signals were stronger at pericentromeric regions than on the chromosome arms. Signals of H3T3ph increased at the pericentromeric region until late metaphase. In contrast, phosphorylated H3T11 was entirely localized on the chromosome from prometaphase to anaphase. Signals of H3T3ph disappeared after chromosome segregation during anaphase, while H3T11ph signals were still localized on the chromosome. A moderate-strength AtHaspin-tdTomato signal was observed on the chromosome during prometaphase and metaphase (Figures 3A and 3B, arrowhead). This localization of AtHaspin-tdTomato overlapped with those of phosphorylated H3T3 and H3T11 from after NEBD until anaphase in BY-2 cells (Figures 4A and 4B).
Then, we observed the localization of AtHaspin-tdTomato in fixed BY-2 cells with paraformaldehyde (Figure 4C). At early prophase before NEBD, AtHaspin-tdTomato was localized in the cytoplasm around the nucleus (Figure 4C; Pro). At prophase after NEBD, AtHaspin-tdTomato enveloped the entire chromosome (Figure 4C; Prometa). This localization also suggested that AtHaspin phosphorylates histone H3 at Thr3 and Thr11 during mitosis.
Expression of AtHaspin in developing organs
According to the microarray data publically available at Genevestigator , AtHaspin is expressed at relatively high levels in the root tips and shoot apex. We plotted the RNA profiles using published microarray datasets . Expression of AtHaspin was activated at 8 to 16 h after removal of the DNA synthesis inhibitor, aphidicolin (Figure 5A). This profile of AtHaspin expression was very similar to those of AtAURs and mitotic AtCycB1;3, indicating that AtHaspin is a mitotic-specific kinase. RT-PCR analyses showed that AtHaspin was expressed in multiple tissues (Figure 5B). Although Haspin was first identified as a testis-specific gene in mice [17, 18], this expression profile indicated that AtHaspin is not a reproduction-specific gene in A. thaliana. AtHaspin showed relatively high expression in flower buds and flowers with high cell division activity.
To investigate the expression patterns of AtHaspin in Arabidopsis plants, we produced transgenic plants expressing AtHaspin-GFP under the control of a native promoter region (the region 1672-bp upstream of the translation initiation codon of AtHaspin). AtHaspin-GFP was expressed in meristems and primordia of root tips, lateral roots (Figures 5C and 5D), the shoot apex, leaf (Figures 5E-5H), and flowers (Figures 5I-5K). Fluorescent signals of AtHaspin-GFP were observed in ovules (Figure 5J). During embryogenesis, AtHaspin-GFP was expressed in embryos and suspensors from the one-cell stage to the four-cell stage (Figures 5L and 5M). The expression of AtHaspin-GFP persisted in the torpedo-stage embryo (Figures 5N and 5O). Thus, the expression patterns of AtHaspin-GFP were strongly correlated with cell division during organ development.
To study the subcellular localization of AtHaspin in detail, we performed time-lapse analysis of AtHaspin-GFP in root tips and the embryo. At interphase, the fluorescent signals of AtHaspin-GFP were observed in the cytoplasm. During mitosis, AtHaspin-GFP was invaded the nucleus at prometaphase and expanded toward the cell periphery at cytokinesis (Additional files 2, 3 and 4). These localization patterns corresponded to those observed in BY-2 cells.
Inducible AtHaspin-KD decreases meristem size in roots
We searched T-DNA tagging lines to elucidate the function of AtHaspin; however, there were no AtHaspin knockout mutants. Considering the possibility that the loss-of function mutant of AtHaspin is embryonic lethal, we constructed a line with chemical-inducible overexpression of a kinase domain mutant of AtHaspin (AtHaspin-KD) using the estradiol-inducible XVE system . When grown on vertically oriented plates with 10 μM 17-β-estradiol, AtHaspin-KD-Venus plants exhibited decreased primary root growth from 11 days after imbibition, compared with root growth of Col. and AtHaspin-Venus plants (Figures 6A and 6B). To investigate the effect of overexpression of AtHaspin-KD-Venus on root tip cells, 6-day-old roots were stained with 4',6-diamidino-2-phenylindole (DAPI) to observe the meristem by detecting the DNA ploidy level of the cells. The root meristem was smaller in AtHaspin-KD-Venus plants than in Col. plants, but the meristem was not affected in AtHaspin-Venus plants (Figures 7A and 7B). Moreover, most AtHaspin-KD-Venus plants showed abnormally oriented cell plates (4/5 plants, Additional file 5). These results suggested that misoriented cell divisions were responsible for the abnormal cell pattern in the root tips. These phenotypes were not observed in transgenic plants without induction of AtHaspin-Venus and AtHaspin-KD-Venus (Figure 6C, 7C, and 7D). AtHaspin-GFP was expressed in the meristem, but not in the quiescent center (QC) or the columella of root tips (Figure 7E). These results suggest that AtHaspin may have a role in mitotic cell division.
AtHaspin phosphorylates histone H3 at both Thr3 and Thr11 in vitro
In this study, we identified a plant Haspin kinase in A. thaliana. Haspin kinases are conserved from yeast to human. Most organisms have one Haspin kinase gene, except for C. elegans and S. cerevisiae. Some plant species, including A. thaliana, have one Haspin candidate gene (Figure 1A). Even in the kinase domain, there is low homology (37%) between AtHaspin and human Haspin. However, the important residues for kinase activity, such as those associated with ATP/Mg2+ ion-binding, are well-conserved in A. thaliana (Figure 1C).
As expected, GST-AtHaspin phosphorylated histone H3 at Thr3 in vitro. Surprisingly, GST-AtHaspin was also able to phosphorylate histone H3 at Thr11 in vitro. It has not been determined whether the Haspin of fission yeast or Xenopus can phosphorylate histone H3 at Thr11, however, the human Haspin phosphorylates histone H3 at Thr3, but not Thr11 in vitro [13, 22, 24]. In the case of Aurora kinase, because its consensus sequence is (RXS/T), AtAUR3 can phosphorylate histone H3 at Ser10 (A7R8K9S10) and Ser28 (A25R26K27S28) . However, the amino acid sequence around Thr3 (A1R2T3K4) differs from that around Thr11 (K9S10T11G12). Point mutational analysis of human Haspin revealed the important residues for histone H3 phosphorylation in the catalytic cleft . These residues are almost all conserved in A. thaliana (Figure 1C, green), with only one residue differing between human (Asp709) and Arabidopsis (Tyr507). The human Haspin mutant D709N shows reduced affinity for the histone H3 tail and impaired ability to phosphorylate H3 . Although AtHaspin can phosphorylate histone H3, it is possible that its substrate specificity is wider than that of the human Haspin. In mammalian cells, the Dlk/ZIP kinase phosphorylates histone H3 at Thr11 in vitro , but there is no direct evidence that it phosphorylates histone H3 at Thr11 in vivo. However, the centromeric localization of Dlk/ZIP during mitosis suggests that this kinase is responsible for H3T11ph. The A. thaliana genome contains no Dlk/ZIP kinase orthologues, and thus, AtHaspin has an additional role as a H3 Thr11 kinase in A. thaliana.
Phosphorylation of histone H3 at Thr3 and Thr11
Mitotic phosphorylation of histone H3 at Ser10, Ser28, Thr3, and Thr11 is highly conserved among eukaryotes. Although phosphorylation of histone H3 was first observed more than 30 years ago, the functions of this modification remain unclear . There is an apparent correlation between H3S10ph and chromosome condensation during mitosis, suggesting that H3S10ph is important for chromosome structure. However, H3S10ph is not required for chromosome condensation in some species . In animals, H3S10ph and H3S28ph occur along the entire chromosome, while H3T3ph and H3T11ph occur only at pericentromeric regions. The functions of H3T3ph and H3T11ph have not been characterized. In contrast to the case in animals, H3T3ph and H3T11ph are preferentially distributed along the entire chromosome in plants (Figures 4A and 4B). Thus, the localization and timing of histone H3 Ser and Thr phosphorylation during mitosis differ between plants and animals. However, the fact that Haspin and Aurora kinases are responsible for histone H3 phosphorylations in plants and animals suggests that the functions of histone H3 phosphorylations are conserved among eukaryotes.
Fluorescent protein fused to AtHaspin (AtHaspin-FP) was localized in the cytoplasm during interphase until NEBD. The interphase localization of AtHaspin differs from that of human Haspin, which localizes in the nucleus during interphase . After NEBD, AtHaspin-FP invaded into the nucleus and spread along the chromosomes and throughout the cytoplasm until metaphase. A strong AtHaspin-FP signal was observed on the chromosome during prometaphase and metaphase. These localization patterns of AtHaspin-FP were consistent with that of AtHaspin-GFP driven by the native promoter in A. thaliana (Additional files 2, 3 and 4). Although no contigs containing Haspin orthologues were found in the BY-2 EST database , AtHaspin shares 61% amino acid sequence identity in the kinase domain to a tomato Haspin orthologue from the Kazusa Tomato SBM Database , suggesting that the localization and dynamics of AtHaspin-FP reflect those of an endogenous Haspin protein in BY-2 cells.
This localization of AtHaspin-FP is consistent with that of phosphorylated H3T3 and H3T11 in BY-2 cells (Figures 3 and 4). The timing of phosphorylation and dephosphorylation of H3T3 and H3T11 are distinct during mitosis. The H3T3ph begins at early prophase before NEBD, while H3T11ph occurs at prophase after NEBD. Dephosphorylation of H3T3 occurs at anaphase, while that of H3T11 occurs in telophase. These results suggested that there are different phosphatases responsible for H3T3 and H3T11 dephosphorylation in plants. We cannot exclude the possibility that there is another histone H3 Thr3 and Thr11 kinase in addition to AtHaspin, because AtHaspin was in the cytoplasm, but H3T3ph signals were detected before NEBD. Another possibility is the specificity of the antibodies against H3T3ph and H3T11ph. The in vitro kinase assay revealed that the antibodies against H3T3ph and H3T11ph did not show cross-reactivity with other histone H3 phosphorylations (Figure 2B and 2C). However, we do not know whether these antibodies react with other histone modifications in vivo. An Aurora kinase inhibitor prevents both H3S10ph and H3S28ph, even though these two phosphorylation events occur at different times during mitosis in tobacco BY-2 cells . Similarly, AtHaspin could phosphorylate histone H3 at Thr3 and Thr11 in planta.
AtHaspin is involved in cell division
Because of the presence of the plant cell wall, cell division, including cytokinesis, is more complex in plants than in animals. A plant-specific cytoskeletal structure, the phragmoplast, is required for completion of cytokinesis in plants . AtHaspin-tdTomato was localized at the phragmoplast after chromosome segregation. Although the phragmoplast expanded to the cell wall, AtHaspin-tdTomato remained around the cell plate (Figure 3C; f). These dynamics of AtHaspin-tdTomato localization and movement suggested that AtHaspin is associated with cell division.
Analyses of Arabidopsis plants expressing AtHaspin-GFP driven by its own promoter indicated that AtHaspin is expressed in root meristems, shoot meristems, floral meristems, and embryos with high cell-division activities. In addition, overexpression of AtHaspin-KD-Venus resulted in defects in root growth. Plant growth is regulated by cell division and cell expansion, and defects in these processes resulted in delayed root growth. Cell expansion is regulated by microtubule-associated proteins (MAPs) . MAPs have roles in microtubule polymerization, depolymerization, bundling, and nucleation. However, no filamentous structures of fluorescent protein-fused AtHaspin were observed at interphase or during mitosis in Arabidopsis and BY-2 cells (Figure 3, Additional files 1, 2, 3 and 4). The expression of AtHaspin-GFP in the root meristem, except for QC and columella cells, indicated that AtHaspin may have a role in cell division during mitosis (Figure 7E). Furthermore, some abnormally oriented cells were observed in root rips of plants overexpressing AtHaspin-KD-Venus (Additional file 5). Although further analysis of down-regulation of AtHaspin is needed, these results suggested that AtHaspin-KD-Venus may inhibit cell division in a dominant negative manner.
In this study, the Haspin kinase in A. thaliana was identified as a mitotic histone H3 threonine kinase. The expression and dominant-negative analysis showed that AtHaspin may have a role in cell division during mitosis. The functions of H3 threonine phosphorylation remain obscure in animals and plants. Recent studies have shown that H3T3ph by S. cerevisiae, Xenopus and human Haspin is required for the accumulation of Aurora B on the centromere, and for subsequent activation of Aurora B kinase activity [22–24]. Therefore, AtHaspin and AtAUR3 coordinately regulate cell division during mitosis via H3 phosphorylation. As previously described [11, 41], homologues of CPC components (INCENP, Survivin, and Borealin/DasraB) could not be found in plants via sequence similarities. However, the CPC has an important role in proper chromosome segregation via regulation of Haspin and Aurora B in animals and yeast [22–24]. The functions and localizations of Haspin and Aurora kinases are partly conserved in A. thaliana [8, 10–12, 42], suggesting that functional analogues of CPC components exist in plants. Further analyses of the substrates of AtHaspin and their downregulating mechanisms will provide insights into the regulation of cell division during plant growth and development.
A. thaliana (ecotype Columbia) seeds were grown on plates containing half-strength Murashige and Skoog salts, Gamborg B5 vitamins, 0.05% MES-KOH (pH 5.8), and 1% agar in a growth chamber at 22°C under continuous light. Tobacco 'Bright Yellow-2' (BY-2) cells were maintained as previously described by Kurihara et al. .
A full-length cDNA for the candidate Haspin kinase (RAFL15-11-J01) was obtained from the RIKEN BioResource Center . Site-directed mutation of AtHaspin for P454L was generated by PCR-based mutagenesis. For construction of the GST fusion protein, the cDNA of AtHaspin was cloned into the pDEST15 expression vector by Gateway technology (Invitrogen). Site-directed mutations of GST-AtHaspin for K309A and of the GST-H3 tail for R2A, T3A, K4A, S10A, T11A, and G12A were generated by PCR-based mutagenesis from the GST-AtHaspin and GST-H3 tail expression vectors, as described elsewhere .
AtHaspin cDNA was cloned into the spUC-GFP vector, which contains the CaMV 35S promoter. spUC-AtHaspin-GFP was digested and ligated with pEBis-kH2 as described by Fujimoto et al. .
For inducible expression constructs, AtHaspin cDNA was cloned into the spUC-tdTomato vector . For Venus expression constructs, tdTomato was replaced by Venus from mVenus/pRSETB . PCR fragments of AtHaspin-tdTomato (or Venus) were cloned into pX7-GFP .
For construction of the GFP expression vector driven by the AtHaspin native promoter, the promoter and genomic regions were amplified from A. thaliana genomic DNA. PCR products were cloned into pENTR using a pENTR/D-TOPO cloning kit (Invitrogen), and then into the pGWB4 expression vector by the LR reaction of the Gateway Cloning System (Invitrogen).
In vitrokinase assay
The in vitro kinase assay was performed with purified GST-AtHaspin or GST-AtHaspin-KD, the GST-histone H3 tail, and mutants as substrates as previously described by Kurihara et al. .
Transformation of BY-2 cells and BY-GT16 cells  was carried out using Agrobacterium-mediated methods as described previously . Stable transgenic BY-2 cells expressing AtHaspin-GFP were observed using a fluorescence microscope (IX-81; Olympus). For induction of AtHaspin-tdTomato, 10 μM 17-β-estradiol (Sigma), or 0.1% ethanol (control) was added to 2-day-old cells, and then cells were cultured for a further 48 h. The cells were observed using a fluorescence microscope (IX-81; Olympus) equipped with a Nipkow disk confocal unit (CSU-X1; Yokogawa Electric). Indirect immunofluorescence was performed as described previously .
The floral-dip method was used for Agrobacterium-mediated Arabidopsis transformation . Transgenic Arabidopsis plants expressing AtHaspin-GFP driven by the native promoter were observed using an upright fluorescence microscope (BX-51; Olympus), or FluoView FV1000 (Olympus). Cell walls were stained with 10 μg/ml propidium iodide (PI).
Root phenotype analysis
For induction of AtHaspin-Venus and AtHaspin-KD-Venus, 10 μM 17-β-estradiol (Sigma) was added to MS medium (pH 5.8, 1.5% agar) to induce expression during seed germination in sterile conditions. The root length of vertically grown seedlings was measured with MBF ImageJ software.
The size of root meristems was determined by the position of the first endoduplicated cells, which were identified on the basis of their DNA content. Roots were fixed with 4% paraformaldehyde and 0.1% Triton X-100 for 40 min and stained with CyStain staining solution containing DAPI (Partec) for 5 min. Images were acquired using the IX-81 fluorescence microscope with a 0.5-μm section at the Z axis. These 2D images were used to construct 3D deconvolution images using AutoQuant X (Media Cybernetics). Cell ploidy was calculated using Metamorph (Molecular Devices). When three cells with more than 4C DNA content were aligned continuously, the top cell was defined as the first endoduplicated cell. Cell wall patterning was analyzed using the mPS-PI method . PI-staining images were acquired under a fluorescence microscope (IX-81, Olympus) equipped with a confocal scanning unit (CSU-X1, Yokogawa Electric). Statistical analyses (one-way ANOVA, followed by Bonferroni post-hoc test) were performed using GraphPad Prism version 5.04 for Windows (GraphPad Software).
analysis of variance
basic local alignment search tool
chromosomal passenger complex
expressed sequence tag
green fluorescent protein
phosphorylation of histone H3 at Ser10
phosphorylation of histone H3 at Ser28
phosphorylation of histone H3 at Thr3
phosphorylation of histone H3 at Thr11
nuclear envelope breakdown
Nigg EA: Mitotic kinases as regulators of cell division and its checkpoints. Nat Rev Mol Cell Biol. 2001, 2 (1): 21-32.
Kouzarides T: Chromatin modifications and their function. Cell. 2007, 128 (4): 693-705. 10.1016/j.cell.2007.02.005.
Hendzel MJ, Wei Y, Mancini MA, Van Hooser A, Ranalli T, Brinkley BR, Bazett-Jones DP, Allis CD: Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma. 1997, 106 (6): 348-360. 10.1007/s004120050256.
Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, Okawa K, Iwamatsu A, Okigaki T, Takahashi T, et al: Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation. J Biol Chem. 1999, 274 (36): 25543-25549. 10.1074/jbc.274.36.25543.
Goto H, Yasui Y, Nigg EA, Inagaki M: Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation. Genes Cells. 2002, 7 (1): 11-17. 10.1046/j.1356-9597.2001.00498.x.
Houben A, Wako T, Furushima-Shimogawara R, Presting G, Kunzel G, Schubert II, Fukui K: Short communication: the cell cycle dependent phosphorylation of histone H3 is correlated with the condensation of plant mitotic chromosomes. Plant J. 1999, 18 (6): 675-679. 10.1046/j.1365-313x.1999.00496.x.
Gernand D, Demidov D, Houben A: The temporal and spatial pattern of histone H3 phosphorylation at serine 28 and serine 10 is similar in plants but differs between mono- and polycentric chromosomes. Cytogenet Genome Res. 2003, 101 (2): 172-176. 10.1159/000074175.
Kurihara D, Matsunaga S, Kawabe A, Fujimoto S, Noda M, Uchiyama S, Fukui K: Aurora kinase is required for chromosome segregation in tobacco BY-2 cells. Plant J. 2006, 48 (4): 572-580. 10.1111/j.1365-313X.2006.02893.x.
Zhang X, Li X, Marshall JB, Zhong CX, Dawe RK: Phosphoserines on maize CENTROMERIC HISTONE H3 and histone H3 demarcate the centromere and pericentromere during chromosome segregation. Plant Cell. 2005, 17 (2): 572-583. 10.1105/tpc.104.028522.
Demidov D, Van Damme D, Geelen D, Blattner FR, Houben A: Identification and dynamics of two classes of aurora-like kinases in Arabidopsis and other plants. Plant Cell. 2005, 17 (3): 836-848. 10.1105/tpc.104.029710.
Kawabe A, Matsunaga S, Nakagawa K, Kurihara D, Yoneda A, Hasezawa S, Uchiyama S, Fukui K: Characterization of plant Aurora kinases during mitosis. Plant Mol Biol. 2005, 58 (1): 1-13. 10.1007/s11103-005-3454-x.
Demidov D, Hesse S, Tewes A, Rutten T, Fuchs J, Ashtiyani RK, Lein S, Fischer A, Reuter G, Houben A: Aurora1 phosphorylation activity on histone H3 and its cross-talk with other post-translational histone modifications in Arabidopsis. Plant J. 2009, 59 (2): 221-230. 10.1111/j.1365-313X.2009.03861.x.
Dai J, Sultan S, Taylor SS, Higgins JM: The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes Dev. 2005, 19 (4): 472-488. 10.1101/gad.1267105.
Preuss U, Landsberg G, Scheidtmann KH: Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003, 31 (3): 878-885. 10.1093/nar/gkg176.
Houben A, Demidov D, Rutten T, Scheidtmann KH: Novel phosphorylation of histone H3 at threonine 11 that temporally correlates with condensation of mitotic and meiotic chromosomes in plant cells. Cytogenet Genome Res. 2005, 109 (1-3): 148-155. 10.1159/000082394.
Caperta AD, Rosa M, Delgado M, Karimi R, Demidov D, Viegas W, Houben A: Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant mitosis and meiosis. Cytogenet Genome Res. 2008, 122 (1): 73-79. 10.1159/000151319.
Tanaka H, Yoshimura Y, Nishina Y, Nozaki M, Nojima H, Nishimune Y: Isolation and characterization of cDNA clones specifically expressed in testicular germ cells. FEBS Lett. 1994, 355 (1): 4-10. 10.1016/0014-5793(94)01155-9.
Tanaka H, Yoshimura Y, Nozaki M, Yomogida K, Tsuchida J, Tosaka Y, Habu T, Nakanishi T, Okada M, Nojima H, et al: Identification and characterization of a haploid germ cell-specific nuclear protein kinase (Haspin) in spermatid nuclei and its effects on somatic cells. J Biol Chem. 1999, 274 (24): 17049-17057. 10.1074/jbc.274.24.17049.
Higgins JM: The Haspin gene: location in an intron of the integrin alphaE gene, associated transcription of an integrin alphaE-derived RNA and expression in diploid as well as haploid cells. Gene. 2001, 267 (1): 55-69. 10.1016/S0378-1119(01)00387-0.
Dai J, Sullivan BA, Higgins JM: Regulation of mitotic chromosome cohesion by Haspin and Aurora B. Dev Cell. 2006, 11 (5): 741-750. 10.1016/j.devcel.2006.09.018.
Dai J, Kateneva AV, Higgins JM: Studies of haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion. J Cell Sci. 2009, 122 (Pt 22): 4168-4176.
Yamagishi Y, Honda T, Tanno Y, Watanabe Y: Two histone marks establish the inner centromere and chromosome bi-orientation. Science. 2010, 330 (6001): 239-243. 10.1126/science.1194498.
Wang F, Dai J, Daum JR, Niedzialkowska E, Banerjee B, Stukenberg PT, Gorbsky GJ, Higgins JM: Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis. Science. 2010, 330 (6001): 231-235. 10.1126/science.1189435.
Kelly AE, Ghenoiu C, Xue JZ, Zierhut C, Kimura H, Funabiki H: Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B. Science. 2010, 330 (6001): 235-239. 10.1126/science.1189505.
Higgins JM: Structure, function and evolution of haspin and haspin-related proteins, a distinctive group of eukaryotic protein kinases. Cell Mol Life Sci. 2003, 60 (3): 446-462. 10.1007/s000180300038.
Phytozome v 6.0. [http://www.phytozome.net/]
Higgins JM: Haspin-like proteins: a new family of evolutionarily conserved putative eukaryotic protein kinases. Protein Sci. 2001, 10 (8): 1677-1684. 10.1110/ps.49901.
Eswaran J, Patnaik D, Filippakopoulos P, Wang F, Stein RL, Murray JW, Higgins JM, Knapp S: Structure and functional characterization of the atypical human kinase haspin. Proc Natl Acad Sci USA. 2009, 106 (48): 20198-20203. 10.1073/pnas.0901989106.
Villa F, Capasso P, Tortorici M, Forneris F, de Marco A, Mattevi A, Musacchio A: Crystal structure of the catalytic domain of Haspin, an atypical kinase implicated in chromatin organization. Proc Natl Acad Sci USA. 2009, 106 (48): 20204-20209. 10.1073/pnas.0908485106.
Zimmermann P, Hirsch-Hoffmann M, Hennig L, Gruissem W: GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol. 2004, 136 (1): 2621-2632. 10.1104/pp.104.046367.
Menges M, Hennig L, Gruissem W, Murray JA: Cell cycle-regulated gene expression in Arabidopsis. J Biol Chem. 2002, 277 (44): 41987-42002. 10.1074/jbc.M207570200.
Guo HS, Fei JF, Xie Q, Chua NH: A chemical-regulated inducible RNAi system in plants. Plant J. 2003, 34 (3): 383-392. 10.1046/j.1365-313X.2003.01723.x.
Ohashi S, Sakashita G, Ban R, Nagasawa M, Matsuzaki H, Murata Y, Taniguchi H, Shima H, Furukawa K, Urano T: Phospho-regulation of human protein kinase Aurora-A: analysis using anti-phospho-Thr288 monoclonal antibodies. Oncogene. 2006, 25 (59): 7691-7702. 10.1038/sj.onc.1209754.
Gurley LR, Walters RA, Tobey RA: Sequential phsophorylation of histone subfractions in the Chinese hamster cell cycle. J Biol Chem. 1975, 250 (10): 3936-3944.
Johansen KM, Johansen J: Regulation of chromatin structure by histone H3S10 phosphorylation. Chromosome Res. 2006, 14 (4): 393-404. 10.1007/s10577-006-1063-4.
Dai J, Higgins JM: Haspin: a mitotic histone kinase required for metaphase chromosome alignment. Cell Cycle. 2005, 4 (5): 665-668. 10.4161/cc.4.5.1683.
Matsuoka K, Demura T, Galis I, Horiguchi T, Sasaki M, Tashiro G, Fukuda H: A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells. Plant Cell Physiol. 2004, 45 (9): 1280-1289. 10.1093/pcp/pch155.
Kazusa Tomato SBM Database. [http://www.kazusa.or.jp/tomato/]
Verma DP: Cytokinesis and Building of the Cell Plate in Plants. Annu Rev Plant Physiol Plant Mol Biol. 2001, 52: 751-784. 10.1146/annurev.arplant.52.1.751.
Sedbrook JC, Kaloriti D: Microtubules, MAPs and plant directional cell expansion. Trends Plant Sci. 2008, 13 (6): 303-310. 10.1016/j.tplants.2008.04.002.
Houben A, Demidov D, Caperta AD, Karimi R, Agueci F, Vlasenko L: Phosphorylation of histone H3 in plants--a dynamic affair. Biochim Biophys Acta. 2007, 1769 (5-6): 308-315.
Kurihara D, Matsunaga S, Uchiyama S, Fukui K: Live cell imaging reveals plant aurora kinase has dual roles during mitosis. Plant Cell Physiol. 2008, 49 (8): 1256-1261. 10.1093/pcp/pcn098.
Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K, Oono Y, et al: Functional annotation of a full-length Arabidopsis cDNA collection. Science. 2002, 296 (5565): 141-145. 10.1126/science.1071006.
Kurihara D, Kawabe A, Matsunaga S, Nakagawa K, Fujimoto S, Uchiyama S, Fukui K: Characterization of a splicing variant of plant Aurora kinase. Plant Cell Physiol. 2007
Fujimoto S, Matsunaga S, Yonemura M, Uchiyama S, Azuma T, Fukui K: Identification of a novel plant MAR DNA binding protein localized on chromosomal surfaces. Plant Mol Biol. 2004, 56 (2): 225-239. 10.1007/s11103-004-3249-5.
Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A: A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol. 2002, 20 (1): 87-90. 10.1038/nbt0102-87.
Kumagai F, Yoneda A, Tomida T, Sano T, Nagata T, Hasezawa S: Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells. Plant Cell Physiol. 2001, 42 (7): 723-732. 10.1093/pcp/pce091.
Clough SJ, Bent AF: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998, 16 (6): 735-743. 10.1046/j.1365-313x.1998.00343.x.
Truernit E, Bauby H, Dubreucq B, Grandjean O, Runions J, Barthelemy J, Palauqui JC: High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis. Plant Cell. 2008, 20 (6): 1494-1503. 10.1105/tpc.107.056069.
We are grateful to RIKEN BRC for the RAFL15-11-J01 cDNA clone, Dr. S. Hasezawa for providing the transgenic BY-2 cell line GT-16, Dr. A. Miyawaki for the Venus expression vector, Dr. R. Y. Tsien for the tdTomato expression vector, Dr. N. H. Chua for the pX7-GFP vector, and Reiko Isobe for technical assistance. This work was supported by the Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to S.M., and by grants from the Japan Science and Technology Agency (BIRD and Program-T to S.M.). D.K. was supported by the Research Fellowships for Young Scientists from the Japan Society for the Promotion of Science.
DK conceived and designed the study, carried out the experiments, performed the statistical analyses, and wrote the manuscript. SM participated in the design of the study, carried out the ploidy analysis and the observations of AtHaspin-KD overexpression in root tips, and revised the draft of the manuscript. TO carried out the phenotypic analyses of plants overexpressing AtHaspin-KD. TH and KF participated in the coordination of the study. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: Dynamics of AtHaspin-tdTomato and GFP-α-tubulin in tobacco BY-2 cells. Dynamics of AtHaspin-tdTomato (magenta) and GFP-α-tubulin (green) from late G2 phase to early G1 phase in BY-2 cells after 48-h induction with 10 μM 17-β-estradiol. Images were acquired at 30-s intervals under a 40 × objective lens, and movie is displayed at 15 frames per second (fps). Numbers indicate time (h: min). (MOV 2 MB)
Additional file 2: Dynamics of AtHaspin-GFP in Arabidopsisroot tips. Images were acquired at 1-min intervals under a 40 × objective lens, and movie is displayed at 15 fps. Numbers indicate time (h: min). Arrowheads indicate mitotic cells. (MOV 5 MB)
Additional file 3: Dynamics of AtHaspin-GFP in Arabidopsistorpedo embryo. Images were acquired at 1-min intervals under a 40 × objective lens, and movie is displayed at 10 fps. Numbers indicate time (h: min). Arrowheads indicate mitotic cells. (MOV 3 MB)
Additional file 4: Dynamics of AtHaspin-GFP in Arabidopsisroot tips. Images were acquired at 90-s intervals under a 100 × objective lens, and movie is displayed at 5 fps. Numbers indicate time (h: min). Arrowheads indicate mitotic cells. (MOV 1 MB)
Additional file 5: Abnormality of cell orientation in AtHaspin-KD plants. At 6 days after imbibition, Col. plants and transformants with inducible AtHaspin-KD-Venus vectors with or without induction were analyzed using the mPS-PI method. In AtHaspin-KD plants, arrows indicate abnormalities in orientations of cell walls between transverse neighboring cells. Scale bar: 50 μm. (TIFF 3 MB)
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Kurihara, D., Matsunaga, S., Omura, T. et al. Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase. BMC Plant Biol 11, 73 (2011). https://doi.org/10.1186/1471-2229-11-73
- Root Meristem
- Kinase Assay
- Aurora Kinase
- Pericentromeric Region
- Native Promoter