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Figure 7 | BMC Plant Biology

Figure 7

From: Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase

Figure 7

Overexpression of AtHaspin-KD-Venus decreased the size of the root meristem. (A, C) At 6 days after imbibition, DNA was stained with DAPI in transformants with inducible AtHaspin-Venus and AtHaspin-KD-Venus with (A) or without induction (C). Arrowheads indicate position of first endocycles in the epidermis. Position of first endoduplicated cells was estimated as described in materials and methods. Scale bar: 50 μm. (B, D) Distance of first endoduplicated cells from QC in epidermis and cell cortex in transformants with inducible AtHaspin-Venus and AtHaspin-KD-Venus with (B) or without induction (D). Mean values ± standard error are shown (n = 5 plants). One-way ANOVA with Bonferroni post-hoc test showed a significant difference between AtHaspin-KD-Venus overexpression plants and Col. (p < 0.001). (E) AtHaspin-GFP was expressed in root tips, except for QC and columella. Cell walls were stained with PI. Merged images show AtHaspin-GFP (green) and PI (magenta). Scale bar: 20 μm.

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