- Research article
- Open Access
Genetic modification of alternative respiration in Nicotiana benthamianaaffects basal and salicylic acid-induced resistance to potato virus X
BMC Plant Biology volume 11, Article number: 41 (2011)
Salicylic acid (SA) regulates multiple anti-viral mechanisms, including mechanism(s) that may be negatively regulated by the mitochondrial enzyme, alternative oxidase (AOX), the sole component of the alternative respiratory pathway. However, studies of this mechanism can be confounded by SA-mediated induction of RNA-dependent RNA polymerase 1, a component of the antiviral RNA silencing pathway. We made transgenic Nicotiana benthamiana plants in which alternative respiratory pathway capacity was either increased by constitutive expression of AOX, or decreased by expression of a dominant-negative mutant protein (AOX-E). N. benthamiana was used because it is a natural mutant that does not express a functional RNA-dependent RNA polymerase 1.
Antimycin A (an alternative respiratory pathway inducer and also an inducer of resistance to viruses) and SA triggered resistance to tobacco mosaic virus (TMV). Resistance to TMV induced by antimycin A, but not by SA, was inhibited in Aox transgenic plants while SA-induced resistance to this virus appeared to be stronger in Aox-E transgenic plants. These effects, which were limited to directly inoculated leaves, were not affected by the presence or absence of a transgene constitutively expressing a functional RNA-dependent RNA polymerase (MtRDR1). Unexpectedly, Aox-transgenic plants infected with potato virus X (PVX) showed markedly increased susceptibility to systemic disease induction and virus accumulation in inoculated and systemically infected leaves. SA-induced resistance to PVX was compromised in Aox-transgenic plants but plants expressing AOX-E exhibited enhanced SA-induced resistance to this virus.
We conclude that AOX-regulated mechanisms not only play a role in SA-induced resistance but also make an important contribution to basal resistance against certain viruses such as PVX.
Salicylic acid (SA) is an important defensive signal in plants that is required for elicitor-triggered immunity and the establishment of systemic acquired resistance (SAR)[1–8]. Plants exhibiting SAR possess an enhanced state of protection against a broad spectrum of pathogens, including viruses, oomycetes, fungi and bacteria [3, 5]. SA inhibits various phases of the viral life cycle including replication, cell-to-cell movement and systemic movement. However, the precise effects of SA can differ between various host-virus combinations [9–16].
RNA silencing (also known as post-transcriptional gene silencing) is thought likely to contribute to SA-induced virus resistance although it is unlikely to be the only mechanism involved . RNA silencing is a sequence-specific mechanism regulating the synthesis, stability, and translatability of mRNA molecules that is guided by small RNA molecules in the size range 21-26 nt [reviewed in ref. ]. The involvement of RNA silencing in SA-induced virus resistance was first suggested by the discovery that SA can induce expression of a component of the RNA silencing machinery, RNA-directed RNA polymerase 1 (RDR1) . RDR1 may also contribute to defense through regulation of host mRNAs encoding other defensive factors, for example those involved in jasmonic acid-induced defenses [19–24]. Additional evidence for a role for RNA silencing in SA-mediated defense arose from studies showing that viral silencing suppressor proteins, for example the cucumber mosaic virus (CMV) 2b protein or HC-Pro encoded by potyviruses, interfere with SA-mediated signaling and SA biosynthesis [25–28].
There is evidence that mitochondrial signaling processes regulate some aspects of SA-induced virus resistance [discussed by [1, 3]]. Reactive oxygen species (ROS) are constantly generated within mitochondria as by-products of respiratory electron transport chain activity [29–31]. Perturbation in this ROS pool or in mitochondrial redox can function in intracellular signal transduction and, through the poorly understood process of mitochondrial retrograde regulation, affect the pattern of nuclear gene expression [31–35]. This form of signaling is influenced by the alternative oxidase (AOX). AOX is a mitochondrial enzyme that is the sole component of the alternative respiratory pathway. The functions of the alternative respiratory pathway include negative regulation of mitochondrial ROS production and maintenance of primary metabolism under stress conditions [30, 36–40].
Evidence supporting an additional role for mitochondrial signaling and AOX in virus resistance includes observations that non-toxic levels of respiratory inhibitors such as antimycin A or cyanide induce resistance against several plant viruses [9, 12, 13, 15, 16, 41, 42], and that SA, which is a weak cytochrome pathway inhibitor, induces Aox1a gene expression [36, 43, 44]. Murphy and associates  found that expression of an AOX coding sequence by a TMV-derived expression vector enhanced its spread in N. benthamiana plants. Gilliland and colleagues  found that in directly inoculated leaves, SA- and antimycin A-induced resistance to TMV was transiently enhanced in transgenic plants that had decreased alternative respiratory pathway capacities. However, in transformed tobacco plants that had increased alternative respiratory pathway capacities due to constitutive expression of an Aox1a transgene, the induction of resistance to TMV by antimycin A was inhibited, while SA-induced resistance was not .
To explain these results, it was proposed that SA and antimycin A both stimulate a signaling pathway that is negatively-regulated by AOX. However, RDR1, a factor in resistance to TMV , is also inducible by SA but not by antimycin A [20, 46]. Since RDR1 gene expression is not affected by AOX, it was suggested that this is why an increase in alternative respiratory pathway capacity inhibits antimycin A-induced resistance but does not completely inhibit SA-induced resistance to TMV [20, 46]. To further investigate the AOX-regulated mechanism of resistance to viruses we have used the experimental host plant N. benthamiana. This is highly susceptible to a wide range of viruses, which is explained to some extent by the fact that its RDR1 ortholog, NbRDR1m, encodes an inactive enzyme . Thus, N. benthamiana provides a natural mutant background for exploring the effect of AOX on plant responses to virus infection without the potentially confounding presence of active RDR1.
Construction and characterization of stably transformed N. benthamianaplants with modified alternative respiratory pathway capacity
To increase or decrease alternative respiratory pathway capacity, N. benthamiana plants (non-transgenic or already expressing an RDR1 transgene derived from Medicago truncatula: 22) were transformed with, respectively, either a wild-type tobacco Aox1a cDNA, or a mutant sequence, Aox-E . Aox-E encodes an inactive version of AOX (AOX-E) to act as a dominant negative mutant [45, 47]. Transgenes were fused to an enhanced cauliflower mosaic virus 35S promoter to drive constitutive high-level expression (Additional File 1). Transgene expression and the respiratory characteristics of transgenic plant lines were examined [20, 40, 45] and lines with up- or down-regulated alternative respiratory capacity were selected for subsequent experiments (Table 1).
Altering alternative respiratory pathway capacity affected basal resistance to potato virus X
Modifying alternative respiratory capacity had dramatic effects on disease symptoms in PVX-infected plants (Figure 1) and on virus accumulation in directly-inoculated and systemically infected leaves (Figure 2A). PVX caused mild symptoms in non-transgenic N. benthamiana plants but caused stunting and leaf curling symptoms in Aox-transgenic plants with increased alternative pathway capacities (Figure 1). Accumulation of PVX coat protein was higher in Aox-transgenic plants with than in non-transgenic plants (Figure 2A). Decreasing the alternative pathway capacity by expression of AOX-E did not appear to affect symptom development following infection with PVX (Figure 1). At 14 days post-inoculation significantly lower levels of virus had accumulated in plants expressing AOX-E than those expressing AOX (p = 0.02). Although at 14 days post-inoculation less virus accumulated in plants expressing AOX-E than in non-transgenic plants these differences were not statistically significant (t-test, p = 0.145) (Figure 2B) and were not observed consistently at later time points (Figure 3). These data show that modulating alternative respiratory capacity affects PVX accumulation.
Salicylic acid-induced resistance to PVX is modified in plants with increased and decreased alternative respiratory pathway capacity
Following treatment with SA, PVX coat protein accumulation was monitored in transgenic plants from lines with increased or decreased alternative respiratory capacities at 28 days post-inoculation. Treatment with SA induced resistance to PVX in non-transgenic N. benthamiana but the treatment did not induce resistance to the virus in Aox-transgenic plants (Figure 3). However, as an inducer of resistance to PVX, SA was markedly more effective in these plants than in non-transgenic or Aox-transgenic plants, with virus accumulation decreased to barely detectable levels (Figure 3). These data show that in N. benthamiana plants, suppression of alternative respiratory pathway capacity combined with SA treatment led to greater PVX resistance and that SA-induced resistance to PVX was compromised by enhancement of alternative respiratory pathway capacity. Thus, for PVX, AOX-modulated defensive signalling is the predominant factor in the regulation of SA-induced resistance.
Altering alternative respiratory pathway capacity affects chemical induction of resistance to tobacco mosaic virus in N. benthamiana
Pre-treatment of non-transgenic N. benthamiana plants with SA inhibited TMV-induced symptom development in systemically infected tissues. However, modifying alternative respiratory pathway capacity in transgenic plants did not affect the timing or appearance of systemic disease symptoms induced by TMV regardless of whether or not the transgenic plants had been treated with SA (data not shown), similar to results in tobacco .
In contrast, modifying the alternative respiratory capacity of N. benthamiana plants did affect the induction of resistance by antimycin A and SA to TMV accumulation in directly inoculated leaf tissue (Figure 4). Leaves were infiltrated with a control solution or solutions of antimycin A or SA before inoculation with TMV. Viral coat protein accumulation in these leaves was detected by immunoblot analysis (Figure 4). In Aox-transgenic lines with increased alternative respiratory pathway capacities, antimycin A-induced resistance to TMV was inhibited (Figure 4). However, SA-induced resistance to TMV was not inhibited in these plants (Figure 4A). The concentration of SA (2.5 mM) required to induce resistance to TMV in non-transgenic N. benthamiana was markedly higher than the 0.5 mM concentration that is sufficient to induce resistance to TMV in tobacco  (Figure 4, compare panels A and B). This may reflect the greater susceptibility to virus infection that is characteristic of N. benthamiana , or that inactivation of SA by conversion to its glucoside occurs more effectively in this plant . However, in transgenic plants expressing AOX-E, that have a decreased capacity for alternative respiration, 0.5 mM SA was adequate to induce resistance to TMV infection even though it did not induce resistance to TMV in non-transgenic N. benthamiana leaves (Figure 4B). This was also seen in three other transgenic lines expressing the AOX-E mutant protein (data not shown). Thus, as was found to be the case in tobacco , in N. benthamiana AOX activity can negatively regulate the induction by SA and antimycin A of resistance against TMV. However, the effects of altering Aox gene expression on TMV infection overall were markedly less striking than the effects these alterations had on PVX infection.
The effects of altering Aox and RDR1 gene expression on chemical induction of resistance to tobacco mosaic virus in N. benthamiana
Previously, we hypothesized that the reason that antimycin A-induced TMV resistance, but not SA-induced resistance, is inhibited in Aox-transgenic transgenic plants is because SA triggers increased expression of the antiviral enzyme RDR1, while antimycin A does not induce RDR1 [20, 46]. We investigated the effect of constitutive RDR1 expression on chemically-induced resistance to TMV using plants doubly transformed with Aox-derived transgenes and a transgene expressing the M. truncatula RDR1 sequence (MtRDR1). MtRDR1-transgenic plants are more resistant to TMV-induced disease and so, in contrast to transgenic control and non-transgenic plants, were not killed by systemic infection with TMV (Additional File 2 and ref. 22). However, the expression of MtRDR1 did not result in any marked effect on TMV accumulation in the directly inoculated leaves (Figure 5), suggesting that the major effect of RDR1 is to protect plants from systemic infection. Constitutive expression of MtRDR1 did not prolong the inhibitory effect of SA on TMV accumulation (Figure 5A), nor did it affect the characteristics of antimycin A-induced resistance to TMV. Although the suppression of antimycin A-induced resistance to TMV was slightly stronger in line 22 than in line 2, in neither of these AOX-expressing lines did the constitutive expression of MtRDR1 rescue chemically-induced resistance to the virus (Figure 5B).
In line with a model proposed by Singh and colleagues , modifying alternative respiration by constitutive expression of AOX or the dominant-negative mutant AOX-E affected the outcome of infection by viruses. For PVX, our data indicates that SA-induced resistance is regulated strongly by AOX and, unexpectedly, that this enzyme also plays a role in maintaining basal resistance to this virus. The interactions of these transgenic N. benthamiana plants with TMV are similar to those observed previously in tobacco plants transformed with Aox-derived transgenes [20, 50]. Thus, as in tobacco, SA-induced resistance to TMV was enhanced in N. benthamiana plants with diminished alternative respiratory pathway capacity but apparently unaffected in plants with an enhanced respiratory capacity. Antimycin A-induced resistance to TMV was, respectively, enhanced or inhibited in transgenic plants in which alternative respiratory pathway capacity had been diminished or increased. However, basal resistance to TMV in N. benthamiana was not affected by modification of alternative respiratory capacity.
Previous studies showed that in tobacco, NtRDR1 activity contributes to the maintenance of basal resistance to PVX whilst in N. benthamiana it is NbRDR6, not NbRDR1, which is a major determinant of basal resistance against this virus [19, 22, 51]. Our data suggests that in N. benthamiana the defensive signal transduction pathway regulated by AOX also plays an important role in basal resistance to PVX. This result was unexpected since evidence from Gilliland and colleagues  had indicated that AOX-regulated defensive signaling plays no discernable role in basal resistance to TMV.
The result was further surprising since PVX, like TMV, is a positive-strand RNA virus . Thus, these viruses replicate in a similar fashion, although they have differing strategies for gene expression and cell-to-cell movement [reviewed by ]. It may be that TMV is more effective than PVX at subverting or inhibiting the antiviral mechanisms that are regulated by AOX. TMV, for example, appears to be able to interfere with a wide range of SA-mediated responses through interaction between its replicase proteins and a transcription factor that regulates basal defense . Alternatively, it may be that PVX is able to evade some of the SA-induced resistance mechanisms that inhibit TMV infection. For instance, although the accumulation of TMV and PVX was inhibited by NtRDR1, the RDR1 of Medicago truncatula inhibited TMV accumulation but PVX appears to be unaffected by this RDR1 ortholog [19, 22].
Another possibility is that PVX, but not TMV, elicits a reaction similar or analogous to PAMP-triggered immunity. PAMPs or pathogen-associated molecular patterns are chemical signatures produced by cellular pathogens (bacteria, fungi, etc) that are perceived by receptor-like kinases, resulting in the triggering of localized defense responses including the generation of ROS . These localized defense responses can underlie non-host resistance (in which plants are not susceptible to the pathogen) or provide some minimal level of resistance to infection (basal resistance) in susceptible plants. Typically, PAMPs are quite generic in their nature and for bacterial pathogens include fragments of flagellin or translation factors . Baurès and colleagues  have theorized that in PVX and other potexviruses a highly conserved amino acid sequence or folded structure in the coat protein might function as a PAMP. It is also known that the PVX coat protein interacts with a factor, the RanGAP2 protein, which may act as a target or decoy for the PVX coat protein and (in plants harbouring the Rx resistance gene) may participate in triggering resistance [58–60]. It is possible that interactions of the PVX coat protein with an unknown receptor, or with RanGAP2 in susceptible plants (such as the N. benthamiana used in this study), result in induction of basal resistance mechanisms via the production of ROS. Maxwell and colleagues  showed that AOX can affect ROS levels throughout the cell, so it could be that this signaling mechanism is affected in plants with modified alternative respiration. Possibly consistent with this idea are observations showing that potexvirus infections are associated with complex changes in ROS and in the activity of anti-oxidant enzymes .
Our rationale for using N. benthamiana in this study was that the NbRDR1m gene encodes a protein that lacks RDR activity . Thus, it was envisaged that we would be able to observe the effects of modifying alternative respiratory capacity on the effectiveness of SA-induced resistance to TMV without interference from RDR1 activity. However, we found that although antimycin A-induced resistance to TMV was affected in plants with either increased, or decreased, alternative respiratory capacity, SA-induced resistance to this virus was only affected (enhanced) in plants with a decreased capacity. The results are similar to those seen in transgenic tobacco plants with increased alternative pathway capacities . Performing experiments with plants expressing MtRDR1 constitutively showed that although these plants are more resistant to systemic TMV-induced disease, the expression of this enzyme has no detectable effects on TMV accumulation in the directly-inoculated leaves or on chemically induced resistance in these tissues. Thus, our hypothesis that SA-induced resistance to TMV in tobacco could be a result of the combined action of AOX-regulated mechanisms and RDR1-mediated viral RNA turnover was overly simplistic. Our results with N. benthamiana do not rule out a role for RDR1 activity in SA-induced resistance to this virus, or a role for AOX as a regulator of this resistance. But the results indicate that additional, unknown factors also participate in SA-induced TMV resistance (Figure 6).
The study revealed that for PVX, SA-induced resistance is much simpler in its execution than SA-induced resistance to TMV. For PVX, AOX-modulated defensive signaling is the predominant factor in controlling SA-induced resistance. In conclusion, the results of this study show that AOX-regulated signaling constitutes an important part of plant antiviral resistance. However, it is also clear that the phenomena of SA-induced resistance and basal resistance to viruses both result from the operation of multiple antiviral mechanisms that do not inhibit all viruses to an equal extent.
Plant Growth Conditions
Seeds of Nicotiana benthamiana (Domin.) non-transgenic and transgenic plant lines were, as appropriate, germinated on soil or under sterile conditions on 1% (w/v) agar containing Murashige and Skoog medium (Melford Ltd, Ipswich, UK). For germination of transgenic seed, agar media were supplemented with kanamycin (50 μg.ml-1), hygromycin B (29 μg.ml-1), or both antibiotics as appropriate. After transfer to soil, all plants were maintained in a growth room (Conviron Ltd., Winnipeg, Manitoba, Canada) under a 16 h photoperiod (200 μE.m-2.s-1 of photosynthetically active radiation) at 22°C and 60% relative humidity.
Generation of transgenic tobacco and N. benthamiana constitutively expressing wild-type and mutant Aox1agene sequences
Adapting a method previously used , pDJSnAOX-E was generated by mutagenesis of the Aox1 cDNA insert of pDJSn  to replace the codon for the active site glutamate 221 with one for alanine using the oligonucleotides 5'-GAAGCTGAAAATGCCAGGATGCACCTCATGAC-3' and 5'-GTCATGAGGTGCATCCTGGCATTTTCAGCTTC-3', and the Stratagene Quikchange XL site-directed mutagenesis kit (http://www.stratagene.com/). Agrobacterium tumefaciens strain GV3101 cells were transformed with DNA for pDJSn or PDJSnAOX-E using the freeze-thaw method . The leaf disc method  was used for A. tumefaciens-mediated transformation of N. benthamiana and transgenic N. benthamiana harboring the 35S:MtRDR1 transgene . Conditions used for transformation of N. benthamiana were similar to those used for tobacco , except that shooting and rooting media were modified to contain 1 μM 1-naphthaleneacetic acid. Transformed lines were screened for transgene incorporation and expression using PCR and RT-PCR, respectively. Immunoblot analysis was used to detect constitutive AOX production and altered alternative respiratory pathway capacity was determined using established methods [20, 45, 40, 64]. Lines of doubly-transformed plants derived from MtRDR1-transgenic plants that had been super-transformed with pDJSn were also checked for expression of the MtRDR1 by RT-PCR and using in vitro RDR activity assays as described by Xie et al. .
Virus strains, detection of infection and chemical treatments
TMV (Genus, Tobamovirus; Species, Tobacco mosaic virus) strain U1  and PVX (Genus Potexvirus; Species Potato virus X) strain UK3  were used in this study. Immunoblot detection of TMV and PVX using appropriate anti-coat protein sera followed a previously described method . For quantification of PVX accumulation, leaf samples were homogenized as described by Murphy et al.  and used in double-antibody sandwich-ELISA  using reagents and antibodies and following instructions supplied by Bioreba (http://www.bioreba.ch/). Quantification was achieved with a standard curve using known amounts of purified virus. Data from transgenic plants were assessed for statistically-significant (p < 0.05) differences from non-transgenic controls using two-tailed t-tests [statistical software Genstat® 2010 Thirteenth Edition, © Lawes Agricultural Trust (Rothamsted Research), VSN International Ltd., Hemel Hempstead, UK.; ].
For whole-plant treatments with SA, five-to-six week old N. benthamiana plants were sprayed for four consecutive days with either a control solution [0.05% (w/v) ethanol] or 1 mM SA dissolved in 0.05% (w/v) ethanol before rub inoculation with PVX or TMV on one or two lower leaves as previously described for tobacco [11, 20]. Chemical treatment of leaf tissue by infiltration was carried out as described by Gilliland et al.  using SA or antimycin A concentrations described in Results and figure legends.
Carr JP, Lewsey MG, Palukaitis P: Signaling in induced resistance. Adv Virus Res. 2010, 76: 57-121. full_text.
Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, Uknes S, Ward E, Kessmann H, Ryals J: Requirement of salicylic acid for the induction of systemic acquired resistance. Science. 1993, 261: 754-756. 10.1126/science.261.5122.754.
Hammerschmidt R: Systemic acquired resistance. Adv Bot Res. 2009, 51: 173-222. full_text.
Király L, Hafez YM, Fodor J, Király Z: Suppression of tobacco mosaic virus-induced hypersensitive-type necrotization in tobacco at high temperature is associated with downregulation of NADPH oxidase and superoxide and stimulation of dehydroascorbate reductase. J Gen Virol. 2008, 89: 799-808.
Loebenstein G: Local lesions and induced resistance. Adv Virus Res. 2009, 75: 73-117. full_text.
Malamy J, Carr JP, Klessig DF, Raskin I: Salicylic acid- A likely endogenous signal in the resistance response of tobacco to viral infection. Science. 1990, 250: 1002-1004. 10.1126/science.250.4983.1002.
Métraux JP, Signer H, Ryals J, Ward E, Wyss-Benz M, Gaudin J, Raschdorf K, Schmid E, Blum W, Inverardi B: Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science. 1990, 250: 1004-1006.
Mur LAJ, Bi YM, Darby RM, Firek S, Draper J: Compromising early salicylic acid accumulation delays the hypersensitive response and increases viral dispersal during lesion establishment in TMV-infected tobacco. Plant J. 1997, 12: 1113-1126. 10.1046/j.1365-313X.1997.12051113.x.
Chivasa S, Carr JP: Cyanide restores N gene-mediated resistance to tobacco mosaic virus in transgenic tobacco expressing salicylic acid hydroxylase. Plant Cell. 1998, 10: 1489-1498. 10.1105/tpc.10.9.1489.
Chivasa S, Murphy AM, Naylor M, Carr JP: Salicylic acid interferes with tobacco mosaic virus replication via a novel, salicylhydroxamic acid-sensitive mechanism. Plant Cell. 1997, 9: 547-557. 10.1105/tpc.9.4.547.
Naylor M, Murphy AM, Berry JO, Carr JP: Salicylic acid can induce resistance to plant virus movement. Mol Plant-Microbe Interact. 1998, 11: 860-868. 10.1094/MPMI.1918.104.22.1680.
Love AJ, Yun BW, Laval V, Loake GJ, Milner JJ: Cauliflower mosaic virus, a compatible pathogen of Arabidopsis, engages three distinct defense-signaling pathways and activates rapid systemic generation of reactive oxygen species. Plant Physiol. 2005, 139: 935-948. 10.1104/pp.105.066803.
Love AJ, Laval V, Geri C, Laird J, Tomos AD, Hooks MA, Milner JJ: Components of Arabidopsis defense- and ethylene-signaling pathways regulate susceptibility to Cauliflower mosaic virus by restricting long-distance movement. Mol. Plant-Microbe Interact. 2007, 20: 659-670. 10.1094/MPMI-20-6-0659.
Murphy AM, Carr JP: Salicylic acid has cell-specific effects on Tobacco mosaic virus replication and cell-to-cell movement. Plant Physiol. 2002, 128: 552-563. 10.1104/pp.010688.
Wong CE, Carson RA, Carr JP: Chemically induced virus resistance in Arabidopsis thaliana is independent of pathogenesis-related protein expression and the NPR1 gene. Mol Plant-Microbe Interact. 2002, 15: 75-81. 10.1094/MPMI.2002.15.1.75.
Mayers CN, Lee KC, Moore CA, Wong SM, Carr JP: Salicylic acid induced resistance to Cucumber mosaic virus in squash and Arabidopsis thaliana: Contrasting mechanisms of induction and antiviral action. Mol Plant-Microbe Interact. 2005, 18: 428-434. 10.1094/MPMI-18-0428.
Lewsey MG, Carr JP: DICER-LIKE proteins 2, 3 and 4 are not essential for salicylic acid-mediated resistance against two RNA viruses. J Gen Virol. 2009, 90: 3010-3014. 10.1099/vir.0.014555-0.
Csorba T, Pantaleo V, Burgyán J: RNA silencing: An antiviral mechanism. Adv Virus Res. 2009, 75: 35-71. full_text.
Xie Z, Fan B, Chen C, Chen Z: An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense. Proc Natl Acad Sci USA. 2001, 98: 6516-6521. 10.1073/pnas.111440998.
Gilliland A, Singh DP, Hayward JM, Moore CA, Murphy AM, York CJ, Slator J, Carr JP: Genetic modification of alternative respiration has differential effects on antimycin A-induced versus salicylic acid-induced resistance to Tobacco mosaic virus. Plant Physiol. 2003, 132: 1518-1528. 10.1104/pp.102.017640.
Yu D, Fan B, MacFarlane SA, Chen Z: Analysis of the involvement of an inducible Arabidopsis RNA-dependent RNA polymerase in antiviral defense. Molec Plant-Microbe Interact. 2003, 16: 206-216. 10.1094/MPMI.2003.16.3.206.
Yang SJ, Carter SA, Cole AB, Cheng NH, Nelson RS: A natural variant of a host RNA-dependent RNA polymerase is associated with increased susceptibility to viruses by Nicotiana benthamiana. Proc Natl Acad Sci USA. 2004, 101: 6297-6302. 10.1073/pnas.0304346101.
Pandey SP, Baldwin IT: RNA-directed RNA polymerase 1 (RdR1) mediates the resistance of Nicotiana attenuata to herbivore attack in nature. Plant J. 2007, 50: 40-53. 10.1111/j.1365-313X.2007.03030.x.
Rakhshandehroo F, Takeshita M, Squires J, Palukaitis P: The influence of RNA-dependent RNA polymerase 1 on Potato virus Y infection and on other antiviral response genes. Mol Plant-Microbe Interact. 2009, 22: 1312-1318. 10.1094/MPMI-22-10-1312.
Ji L-H, Ding SW: The suppressor of transgene RNA silencing encoded by Cucumber mosaic virus interferes with salicylic acid-mediated virus resistance. Mol Plant-Microbe Interact. 2001, 14: 715-724. 10.1094/MPMI.2001.14.6.715.
Krečič-Stres H, Vucak C, Ravnikar M, Kovač M: Systemic Potato virus YNTN infection and levels of salicylic and gentisic acids in different potato genotypes. Plant Pathology. 2005, 54: 441-447.
Alamillo JM, Saénz P, García JA: Salicylic acid-mediated and RNA-silencing defense mechanisms cooperate in the restriction of systemic spread of plum pox virus in tobacco. Plant J. 2006, 48: 217-227. 10.1111/j.1365-313X.2006.02861.x.
Lewsey MG, Murphy AM, MacLean D, Dalchau N, Westwood JH, Macaulay K, Bennett MH, Moulin M, Hanke DE, Powell G, Smith AG, Carr JP: Disruption of two defensive signaling pathways by a viral RNA silencing suppressor. Molec Plant-Microbe Interact. 2010, 23: 835-845. 10.1094/MPMI-23-7-0835.
Noctor G, Foyer CH: Ascorbate and glutathione keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol. 1998, 49: 249-279. 10.1146/annurev.arplant.49.1.249.
Yip JYH, Vanlerberghe GC: Mitochondrial alternative oxidase acts to dampen the generation of active oxygen species during a period of rapid respiration induced to support a high rate of nutrient uptake. Physiologia Plantarum. 2001, 112: 327-333. 10.1034/j.1399-3054.2001.1120305.x.
Noctor G, De Paepe R, Foyer CH: Mitochondrial redox biology and homeostasis in plants. Trends Plant Sci. 2007, 12: 125-134. 10.1016/j.tplants.2007.01.005.
Maxwell DP, Nickels R, McIntosh L: Evidence of mitochondrial involvement in the transduction of signals required for the induction of genes associated with pathogen attack and senescence. Plant J. 2002, 29: 269-279. 10.1046/j.1365-313X.2002.01216.x.
Umbach AL, Fiorani F, Siedow JN: Characterization of transformed Arabidopsis with altered alternative oxidase levels and analysis of effects on reactive oxygen species in tissue. Plant Physiol. 2005, 139: 1806-1820. 10.1104/pp.105.070763.
Rhoads DM, Umbach AL, Subbiah CC, Siedow JN: Mitochondrial reactive oxygen species. Contribution to oxidative stress and interorganellar signalling. Plant Physiol. 2006, 141: 357-366. 10.1104/pp.106.079129.
Rhoads DM, Subbiah CC: Mitochondrial retrograde regulation in plants. Mitochondrion. 2007, 7: 177-194. 10.1016/j.mito.2007.01.002.
Maxwell DP, Wang Y, McIntosh L: The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. Proc Natl Acad Sci USA. 1999, 96: 8271-8276. 10.1073/pnas.96.14.8271.
Affourtit C, Krab K, Moore AL: Control of plant mitochondrial respiration. Biochimica et Biophysica Acta- Bioenergetics. 2001, 1504: 58-69. 10.1016/S0005-2728(00)00239-5.
Affourtit C, Albury MSW, Crichton PG, Moore AL: Exploring the molecular nature of alternative oxidase regulation and catalysis. FEBS Lett. 2002, 510: 121-126. 10.1016/S0014-5793(01)03261-6.
Moore AL, Albury MS, Crichton PG, Affourtit C: Function of the alternative oxidase: Is it still a scavenger?. Trends Plant Sci. 2002, 7: 478-481. 10.1016/S1360-1385(02)02366-X.
Pasqualini S, Paolocci F, Borgogni A, Morettini R, Ederli L: The overexpression of an alternative oxidase gene triggers ozone sensitivity in tobacco plants. Plant Cell Environ. 2007, 30: 1545-1556. 10.1111/j.1365-3040.2007.01730.x.
Wang J, Wang X, Liu C, Zhang JD, Zhu CX, Gu XQ: The NgAOX1a gene cloned from Nicotiana glutinosa is implicated in the response to abiotic and biotic stresses. Biosci Rep. 2008, 28: 259-266. 10.1042/BSR20080025.
Fu LJ, Shi K, Gu M, Zhou YH, Dong DK, Liang WS, Song FM, Yu JQ: Systemic induction and role of mitochondrial alternative oxidase and nitric oxide in a compatible tomato-Tobacco mosaic virus interaction. Molec Plant-Microbe Interact. 2010, 23: 39-48. 10.1094/MPMI-23-1-0039.
Xie Z, Chen Z: Salicylic acid induces rapid inhibition of mitochondrial electron transport and oxidative phosphorylation in tobacco cells. Plant Physiol. 1999, 120: 217-225. 10.1104/pp.120.1.217.
Norman C, Howell KA, Millar AH, Whelan JM, Day DA: Salicylic acid is an uncoupler and inhibitor of mitochondrial electron transport. Plant Physiol. 2004, 134: 492-501. 10.1104/pp.103.031039.
Murphy AM, Gilliland A, York CJ, Hyman B, Carr JP: High-level expression of alternative oxidase protein sequences enhances the spread of viral vectors in resistant and susceptible plants. J Gen Virol. 2004, 85: 3777-3786. 10.1099/vir.0.80385-0.
Singh DP, Moore CA, Gilliland A, Carr JP: Activation of multiple antiviral defence mechanisms by salicylic acid. Mol Plant Pathol. 2004, 5: 57-63. 10.1111/j.1364-3703.2004.00203.x.
Herskowitz I: Functional inactivation of genes by dominant negative mutations. Nature. 1987, 329: 219-222. 10.1038/329219a0.
Goodin MM, Zaitlin D, Naidu RA, Lommel SA: Nicotiana benthamiana: Its history and future as a model for plant-pathogen interactions. Molec Plant-Microbe Interact. 2008, 21: 1015-1026. 10.1094/MPMI-21-8-1015.
Henning J, Malamy J, Grynkiewicz G, Indulski J, Klessig DF: Interconversion of the salicylic acid signal and its glucoside in tobacco. Plant J. 1992, 4: 593-600. 10.1046/j.1365-313X.1993.04040593.x.
Ordog SH, Higgins VJ, Vanlerberghe GC: Mitochondrial alternative oxidase is not a critical component of plant viral resistance but may play a role in the hypersensitive response. Plant Physiol. 2002, 129: 1858-1865. 10.1104/pp.003855.
Schwach F, Vaistij FE, Jones L, Baulcombe DC: An RNA dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiol. 2005, 138: 1842-1852. 10.1104/pp.105.063537.
Verchot-Lubicz J, Ye CM, Bamunusinghe D: Molecular biology of the potexviruses: Recent advances. J Gen Virol. 2007, 88: 1643-1655. 10.1099/vir.0.82667-0.
Verchot-Lubicz J: A new cell-to-cell transport model for Potexviruses. Molec Plant-Microbe Interact. 2005, 18: 283-229. 10.1094/MPMI-18-0283.
Wang X, Goregaoker SP, Culver JN: Interaction of the Tobacco mosaic virus replicase protein with a NAC domain transcription factor is associated with the suppression of systemic host defenses. J Virol. 2009, 83: 9720-9730. 10.1128/JVI.00941-09.
Boller T, He S-Y: Innate immunity in plants: An arms race between pattern recognition receptors in plants and effectors in microbial pathogens. Science. 2009, 324: 742-744. 10.1126/science.1171647.
Lindeberg M, Cunnac S, Collmer A: The evolution of Pseudomonas syringae host specificity and Type III effector repertoires. Mol Plant Pathol. 2009, 10: 767-775. 10.1111/j.1364-3703.2009.00587.x.
Baurès I, Candresse T, Leveau A, Bendahmane A, Sturbois B: The Rx gene confers resistance to a range of Potexviruses in transgenic Nicotiana plants. Mol Plant-Microbe Interact. 2008, 21: 1154-1164.
Sacco MA, Mansoor S, Moffett P: A RanGAP protein physically interacts with the NB-LRR protein Rx, and is required for Rx-mediated viral resistance. Plant J. 2007, 52: 82-93. 10.1111/j.1365-313X.2007.03213.x.
Tameling WIL, Baulcombe DC: Physical association of the NB-LRR resistance protein Rx with a Ran GTPase-activating protein is required for extreme resistance to Potato virus X. Plant Cell. 2007, 19: 1682-1694. 10.1105/tpc.107.050880.
Moffett P: Mechanisms of recognition in R gene mediated resistance. Adv Virus Res. 2009, 75: 2-33.
Clarke SF, Guy PL, Burritt DJ, Jameson PE: Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment. Physiologia Plantarum. 2002, 114: 157-164. 10.1034/j.1399-3054.2002.1140201.x.
Holsters M, de Waele D, Depicker A, van Montagu M, Schell J: Transfection and transformation of Agrobacterium tumefaciens. Molec Gen Genetics. 1978, 163: 181-187. 10.1007/BF00267408.
Horsch RB, Fry JE, Hoffman NL, Eichholz D, Rogers SG, Fraley RT: A simple and general method for transferring genes into plants. Science. 1985, 227: 1229-1231. 10.1126/science.227.4691.1229.
Elthon TE, Nickels RL, McIntosh L: Monoclonal antibodies to the alternative oxidase of higher plant mitochondria. Plant Physiol. 1989, 89: 1311-1317. 10.1104/pp.89.4.1311.
Carr JP: Tobacco mosaic virus. Plant-Pathogen Interactions. Edited by: Talbot NJ. Blackwell, Oxford; 2004, 27-67.
Köhm BA, Goulden MG, Gilbert JE, Kavanaugh TA, Baulcombe DC: A potato virus X resistance gene mediates an induced, non-specific resistance in protoplasts. Plant Cell. 1993, 5: 913-920.
Murphy AM, Otto B, Brearley CA, Carr JP, Hanke DE: A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens. Plant J. 2008, 56: 638-652. 10.1111/j.1365-313X.2008.03629.x.
Barbara DJ, Clark MF: A simple indirect ELISA using F(ab')2 fragments of immunoglobulin. J Gen Virol. 1982, 58: 315-322. 10.1099/0022-1317-58-2-315.
Payne RW, Harding SA, Murray DA, Soutar DM, Baird DB, Glaser AI, Channing IC, Welham SJ, Gilmour AR, Thompson R, Webster R: The guide to Genstat release 13, Part 2: Statistics. Oxford: VSN International; 2010.
The authors thank Alex Murphy, Androulla Gilliland and Mat Lewsey for useful discussions and Zhixiang Chen for advice on performing in vitro RDR assays. We thank Rick Nelson for MtRDR1-expressing N. benthamiana and the transformed control line, Tom Elthon for anti-AOX monoclonal antibody, and Mike Wilson for anti-TMV CP polyclonal serum. FSF was funded by grants from the Cambridge Overseas Trust and the Ministry of Education of Taiwan, and WSL was funded by a studentship from the Biotechnology and Biological Sciences Research Council (BBSRC). JVL was funded by a USDA CSREES-NRI-2007-01530 Research Sabbatical Grant. Work in JPC's laboratory is supported by grants from The Leverhulme Trust (F/09 741/F), BBSRC (BB/D008204/1; BB/F014376/1), the EU and the Cambridge University Isaac Newton Trust.
WSL and FSF carried out the experimental procedures, with additional experiments by JVL on PVX accumulation. JPC initiated and directed the research and wrote the manuscript. All authors read and approved the final manuscript.
Wing-Sham Lee, Shih-Feng Fu contributed equally to this work.
Electronic supplementary material
Additional file 1: Detection of AOX-E protein expression and over-expression of AOX protein in transgenic Nicotiana benthamiana plant lines used in this study. Immunoblot detection of AOX (A) or mutant AOX-E (B) present in non-transgenic (NT) N. benthamiana plants and T2 generation transformed plants belonging to various independent lines (numbered) harboring AOX or AOX-E transgenes expressed under the control of the 35S constitutive promoter. Equal amounts of Triton X-100 soluble proteins were denatured in the presence of 0.1 M dithiothreitol and subjected to immunoblot analysis using an anti-AOX monoclonal antibody. Anti-AOX binding was detected using anti-mouse immunoglobulin conjugated to horseradish peroxidase and a chemiluminescent substrate. A protein sample extracted from a plant of the Sn6 transgenic tobacco plant line, which over-expresses AOX (Murphy et al., 2004), served as the Positive Control. The major cross-reacting band in all cases corresponded in size (apparent Mr c.35kDa) to the reduced form of AOX. Pre-stained Mr markers were not visible on the X-ray film. All lines express AOX or AOX-E at much higher levels than the native AOX protein which is not detectable on this western blot. (PDF 152 KB)
Additional file 2: Image showing TMV-induced systemic symptoms of TMV on non-transgenic, control transgenic and MtRDR1-transgenic N. benthamiana. Non-transgenic (NT) Nicotiana benthamiana plants, plants transformed with an 'empty' transformation vector (Control) and transgenic lines constitutively expressing MtRDR1 (35S:MtRDR1: Reference 22) were inoculated with TMV U1 strain (0.05 ug/ml) and photographed four weeks later. Mock-inoculated NT plants are shown for comparison. The scale bar is 8 cm. (PDF 2 MB)
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Lee, WS., Fu, SF., Verchot-Lubicz, J. et al. Genetic modification of alternative respiration in Nicotiana benthamianaaffects basal and salicylic acid-induced resistance to potato virus X. BMC Plant Biol 11, 41 (2011). https://doi.org/10.1186/1471-2229-11-41
- Salicylic Acid
- Tobacco Mosaic Virus
- Basal Resistance
- Benthamiana Plant
- Tobacco Mosaic Virus Infection