Fig. 6

NtPPOs affect pollen growth in the pistil and fruit weights. (a-b) The pollen tube in the style at 5 and 8 HAP. HAP, hours after self-pollination. Bars = 500 μm. (c) Quantification of pollen tube length in the self-pollinated pistil. n = 15 pollinated pistils were measured across three biological replicates, and the error bar represents the standard deviation. Data were analyzed by ANOVA, P < 0.05. (d-e) The semi in vitro test of the pollen tube growth in the pistil at 24 HAP. Bars = 500 μm. n = 15 pollinated pistils were measured across three biological replicates, and the error bar represents the standard deviation. Data were analyzed by ANOVA, P < 0.05. (f-g) Mature fruits and quantification of fruit weight. Bars = 1 cm. n = 30 fruits were measured across three biological replicates; each group contains 10 fruits. Error bars indicate standard deviation of three biological replicates. Data were analyzed by ANOVA, P < 0.05