Fig. 5
From: The PPO family in Nicotiana tabacum is an important regulator to participate in pollination
Expression and distribution analysis of NtPPOs in the N.tabtacum self-pollinated stigma/style. For negative control, the primary antibody was replaced with 1×PBS buffer. (a) The morphology of stigma and style in N.tabtacum and IF analysis of NtPPO contents and distribution in the un-pollinated stigma/style, and in the N.tabtacum self-pollinated stigma/style at 2.5, 5, 10, and 15 HAP. NP, un-pollinated; HAP, hours after pollination; Pc, papillae cell; TTS, transmitting tissue; Pt, parenchymatous tissue. The red box indicates the observation sites. Red arrow indicates NtPPO fluorescent signals. Scale bar: 50 μm. (b-c) Quantification of green fluorescence signals intensity. The error bar represents standard deviation from three repeats, ** P < 0.01, Student’s t-test. (d) NtPPOs activity in the N.tabtacum NP stigma/style and self-pollinated stigma/style.Three biological replicates were performed. Error bars with different letters are significantly different from each other (P < 0.05). Data were analyzed by ANOVA