Fig. 4
From: The PPO family in Nicotiana tabacum is an important regulator to participate in pollination
Expression and distribution analysis of NtPPOs in the reproductive tissues by IF. For negative control, the primary antibody was replaced with 1×PBS buffer. (a) In vitro growing pollen tubes. The arrow indicates NtPPOs in the pollen tube tip. (b) Stigma micrograph. The arrow shows the NtPPOs in the interface of the papillae cell layer and transmitting tissue. (c) Style micrograph. The arrow shows the NtPPOs in the transmitting tissue and parenchymatous tissue. (d) Ovary micrograph. The arrow shows NtPPOs in the ovary wall and ovule. Ct, connective tissue; Aw, anther wall; Pc, papillae cell; Pt, parenchymatous tissue; TTS, transmitting tissue