Table 5 Primer sequence of the studied polyphenol biosynthetic pathways genes
Primer name | Abbreviation |  | (5’-3’) | Product size (bp) | Melting Temp (°C) | Reference |
|---|---|---|---|---|---|---|
Phenylalanine ammonia lyase 1 | PAL1 | F R | ACGGGTTGCCATCTAATCTGACA CGAGCAATAAGAAGCCATCGCAAT | 92 | 80 | AY005474 |
Cinnamic acid 4-hydroxylase | C4H | F R | CCCAGT TTTTGGAAATTGGCTTCA GCCCCATTCTAAGCAAGAGAACAT C | 104 | 75 | CK157495 |
Chalcone synthase | CHS | F R | CACCGTGGAGGAGTATCGTAAGGC TGATCAACACAGTTGGAAGGCG | 93 | 82 | EU408770 |
Chalcone isomerase 2 | CHI2 | F R | GGCAGGCCATTGAAAAGTTCC CTAATCGTCAATGATCCAAGCGG | 103 | 78 | AB187026 |
Flavanone 3-hydroxylase | F3H | F R | CCAAGGCATGTGTGGATATGGACC CCTGGATCAGTATGTCGTTCAGCC | 103 | 78 | AB187027 |
Flavonoid 3’-hydroxylase | F3’H | F R | TGGGTATACCCAAACTCATTCCG AAAAGCCCAAAGTTGATGTGAAAGG | 96 | 77 | AY519468 |
Dihydroflavonol-4-reductase | DFR | F R | TCACAGGAGCAGCTGGATTTATCG TCAGGATCACGAACAGTAGCATGG | 91 | 79 | AY209183 |
Flavonol synthase 1 | FLS1 | F R | CCTCCTTCCTACAGGGAAGCAAA CAAGCCCAAGTGACAAGCTCCTAA | 91 | 79 | KJ193852 |
Anthocyanin 1 | AN1 | F R | CCTCAACCTCAGAAATTCAGAAGC TCGTTGTTGTTGTCGTTCGATGC | 102 | 75 | MG979067 |
Anthocyanin 2 | AN2 | F R | ACAAGATGCCACTTTCCTTCACC TGTGCATCGTTGGGAGTTAGG | 101 | 75 | XM_044522067 |
Hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase | HQT | F R | CCCAATGGCTGGAAGATTAGCTA CATGAATCACTTTCAGCCTCAACAA | 99 | 76 | OP295931 |
Hydroxycinnamoyl-CoA shikimate hydroxycinnamoyl transferase | HCT | F R | TCTCCAACCCCTTTTAACGAACC CAACTTGTCCTTCTACCACAGGGAA | 103 | 80 | OP295858 |
4-coumarate 3-hydroxylase | C3H | F R | TTGGTGGCTACGACATTCCTAAGG GGTCTGAACTCCAATGGGTTATTCC | 100 | 82 | CD895891 |
α-tubulin (Reference gene) | α-tubulin | F R | TATCTGCTACCAGGCTCCCGAGAA TGGTGTTGGACAGCATGCAGACAG | 116 | 85 |  |
β-actin (Reference gene) | β-actin | F R | GTGGGCCGCTCTAGGCACCAA CTCTTTGATGTCACGCACGATTTC | 540 | 88 |  |