Panax notoginseng transcription factor WRKY15 modulates resistance to Fusarium solani by up-regulating osmotin-like protein expression and inducing JA/SA signaling pathways
BMC Plant Biology volume 23, Article number: 362 (2023)
Panax notoginseng (Burk) F. H. Chen is a valuable traditional Chinese medicinal plant, but its commercial production is seriously affected by root rot caused by some pathogenic fungi, including Fusarium solani. Nevertheless, the genetic breeding for disease resistance of P. notoginseng remains limited. The WRKY transcription factors have been revealed to play important roles in plant defense responses, which might provide an inspiration for resistance improvement in P. notoginseng.
In this study, the regulatory mechanism of transcription factor PnWRKY15 on P. notoginseng resistance to F. solani infection was revealed. The suppressed expression of PnWRKY15 via RNA interference increased the sensitivity of P. notoginseng to F. solani and decreased the expression levels of some defense-related genes, including PnOLP1, which encodes an osmotin-like protein that confers resistance to F. solani. Ectopic expression of PnWRKY15 in the model plant tobacco significantly enhanced the resistance to F. solani. Moreover, the transcriptome sequencing analysis discovered that some pathogenesis-related genes were expressed at higher levels in the PnWRKY15-overexpressing tobacco than that in the wild-type tobacco. In addition, the jasmonic acid (JA) and salicylic acid (SA) signaling pathways were evidently induced by PnWRKY15-overexpression, that was evidenced by that the JA and SA contents were significantly higher in the PnWRKY15-overexpressing tobacco than that in the wild-type. Furthermore, PnWRKY15, which was localized in the nucleus, can trans-activate and up-regulate PnOLP1 expression according to the EMSA, yeast one-hybrid and co-expression assays.
PnWRKY15 contributes to P. notoginseng resistance to F. solani by up-regulating the expression of resistance-related gene PnOLP1 and activating JA/SA signaling pathways. These findings will help to further elucidate the transcriptional regulatory mechanism associated with the P. notoginseng defense response to F. solani.
WRKY transcription factors (TFs), which belong to one of the largest TF families, contain a highly conserved DNA-binding heptapeptide (WRKYGQK) and a zinc finger motif. They actively respond to pathogen infections and reprogram the transcriptome to trigger the plant immune response. The expression levels of most of the identified WRKY genes in pepper (Capsicum annuum) are up-regulated in plants inoculated with Phytophthora capsicum and pepper mottle virus . A total of 42 Akebia trifoliata WRKY genes have been characterized, of which AktWRKY11/18/21/31/47- 2/51/65/70/74 were up-regulated in response to a Colletotrichum acutatum infection . Additionally, 23 WRKY genes have been identified in the Botrytis-resistant (Botrytis elliptica and Botrytis cinerea) species Lilium regale; the ectopic expression of LrWRKY4 and LrWRKY12 in Arabidopsis thaliana leads to enhanced resistance to B. cinerea .
WRKY TFs bind to the cis-element W-box [(C/T)TGAC(T/C)] in target gene promoters to regulate expression . Changes to any of the nucleotides in the core sequence (TGAC) greatly hinder the ability of WRKY TFs to bind to the W-box cis-element . Apple (Malus domestica) WRKY31 binds specifically to the W-box2 cis-element in the promoter of MdHIR4, which encodes a negative regulator in pathogenic resistance to Botryosphaeria dothidea, and then represses MdHIR4 expression, thereby enhancing apple resistance to B. dothidea . Four cotton (Gossypium hirsutum) group IIc WRKY TFs bind directly to the W-box element in the GhMKK2 promoter to positively regulate its expression, leading to increased resistance to Fusarium oxysporum f. sp. vasinfectum .
Earlier research indicated that WRKY TFs and signal transduction pathways associated with various hormones, including jasmonic acid (JA), ethylene (ET), salicylic acid (SA), and abscisic acid (ABA), cooperatively mediate plant stress responses . The expression of WRKYs in lotus (Nelumbo Nucifera) roots was regulated by JA and SA signaling, especially for NnWRKY40a and NnWRKY40b, both of which were significantly induced after JA treatment . In oil palm (Elaeis guineensis), WRKY gene expression is induced in response to various concentrations of ABA, methyl jasmonate (MeJA), SA, and H2O2 following an exposure to drought stress . Notably, JA and SA are critical hormones mediating signal transduction after a pathogen infection . The JA signaling pathway generally affects the defense response to herbivores and necrotic pathogens, whereas the SA signaling pathway usually mediates the defense response to biotrophic pathogens . However, the cross-talk between different hormone (e.g., JA and SA) signaling pathways is relatively complex. The regulatory effects of JA and SA signaling pathways on plant immune responses to pathogens are antagonistic or synergistic .
Panax notoginseng (Burk) F. H. Chen is an important medicinal plant that is traditionally used to promote blood circulation, stop bleeding, and enhance the human immune system . The medicinal materials derived from P. notoginseng are mainly produced in Wenshan prefecture, Yunnan province, China. As a perennial species, 3-year-old P. notoginseng plants may be used to produce medicine. Unfortunately, P. notoginseng plants are susceptible to many pathogens during their long growth period. For example, Fusarium species, including Fusarium solani, are responsible for P. notoginseng root rot, which can significantly decrease plant yield and quality . The application of fungicidal chemicals is the traditional method used to control root rot, but the available chemicals have limited efficacy. Moreover, these chemicals may pollute the soil environment and result in accumulation of medicinal products unsafe for human consumption. To develop an environmentally friendly and effective method for controlling P. notoginseng root rot, the mechanism underlying the plant defense response to the disease must be elucidated.
Previous studies confirmed that an exogenous MeJA treatment increases the resistance of P. notoginseng to F. solani. Furthermore, a series of JA-responsive F. solani resistance-related genes in P. notoginseng has been isolated, including osmotin-like protein (PnOLP1) . The PnOLP1 expression level is significantly up-regulated by MeJA and SA treatments as well as by F. solani infections. The encoded protein, which is localized in the cell wall, can inhibit F. solani, F. oxysporum, and Fusarium graminearum mycelial growth in vitro. The ectopic expression of PnOLP1 in tobacco (Nicotiana tabacum) reportedly increases the resistance to F. solani . The resistance induced by the application of exogenous MeJA is accompanied by the activated expression of many WRKY genes, including PnWRKY9 and PnWRKY15 . Additionally, PnWRKY9 and the JA signaling pathway synergistically enhance root rot resistance by regulating the expression of a defensin gene, PnDEFL1 . The WRKY TF-mediated network regulating plant defense responses to pathogens is extremely complex. Therefore, in this study, we explored the molecular basis of PnWRKY15 in response to an infection by F. solani in P. notoginseng.
P. notoginseng WRKY15 is a nuclear protein
The P. notoginseng WRKY gene family was identified in . In the current study, we focused on the regulatory effect of PnWRKY15 on the defense response to root rot. The PnWRKY15 cDNA was 960 bp long, with a 420 bp open reading frame (ORF) encoding a protein comprising 139 amino acid residues. The deduced protein (PnWRKY15) was predicted to be 15.87 kDa, with an isoelectric point of 7.51. The sequence analysis revealed PnWRKY15 contains a conserved heptapeptide (WRKYGQK), which is followed by a zinc finger motif (C-X4-5-CX22-23-H-X1-H; X refers to any amino acid) (Fig. 1A). In addition, PnWRKY15 is highly homologous to Jatropha curcas WRKY45 (XP_012075800.1), Quercus lobata WRKY3 (XP_030954519.1), and Manihot esculenta WRKY28 (XP_021596011.1), with sequence identities of 87%, 75%, and 69%, respectively. The PnWRKY15-GFP fusion cascade was expressed in onion epidermal cells to clarify the subcellular localization of PnWRKY15. The fluorescence of PnWRKY15-GFP was detected in the nucleus. The subcellular localization result was confirmed by the colocalization of propidium iodide (PI, a nucleus-staining dye) (Fig. 1B).
Down-regulation of PnWRKY15 expression via RNA interference (RNAi) increased the susceptibility of P. notoginseng to F. solani
There is currently a lack of a practical and efficient method for the genetic transformation in P. notoginseng. An RNAi fragment targeting PnWRKY15 was transiently expressed in the young leaves of P. notoginseng plants to determine whether the gene is involved in defense response to F. solani. The quantitative real-time polymerase chain reaction (qRT-PCR) data indicated that the PnWRKY15 expression level was approximately 50% lower in the P. notoginseng leaves expressing the RNAi fragment than that in the control P. notoginseng leaves carrying the empty vector (Fig. 2A), reflecting a successful RNAi-based down-regulation of PnWRKY15 expression in P. notoginseng leaves. After inoculation with F. solani, PnWRKY15 expression was induced in the RNAi plants, but its expression level was still significantly lower than that in the control plants. In terms of the symptoms caused by the F. solani infection, the decayed area was larger in the leaves of RNAi plants than that of the control (Fig. 2B-C). The expression levels of JA/SA signaling-related genes, including PnAOS, PnMYC2, and PnPR-1, as well as two pathogenesis-related (PR) genes (PnCHI and PnOLP1), decreased in the RNAi P. notoginseng plants (Fig. 2D). Accordingly, the down-regulated expression of PnWRKY15 increased the susceptibility of P. notoginseng to F. solani and suppressed the expression of some disease resistance related genes. These findings suggest that PnWRKY15 encodes a positive regulator in P. notoginseng defense response against F. solani.
Ectopic expression of PnWRKY15 in tobacco enhanced the resistance to F. solani
An exogenic gene can be stably expressed in tobacco through Agrobacterium mediated genetic transformation, which is a more reliable method to accurately uncover the function of that gene. In addition, the F. solani causes root rot in tobacco . Therefore, the PnWRKY15 gene was ectopically expressed in the model plant tobacco to verify its function. The T2 generation of PnWRKY15-overexpressing (OE) tobacco lines were developed and examined. Firstly, 15 tobacco lines were selected for the analysis of PnWRKY15 expression. The qRT-PCR data confirmed that PnWRKY15 was significantly over expressed in all of the tested OE lines, although the transcript levels varied (Fig. 3A). More specifically, the PnWRKY15 expression level was highest in line 15 - 2, with a relative expression level of 5.44. Other OE lines, including 15 - 11/-15/-21/-22/-23/-24, had a relative expression level of 2.90, 3.01, 4.07, 3.31, 3.07, and 3.47, respectively.
The resistance of the PnWRKY15-OE tobacco lines to F. solani was subsequently evaluated. Four T2 PnWRKY15-OE tobacco lines (15 - 2/-21/-22/-24) were tested. After a 7-day incubation of tobacco roots with F. solani, the leaves of wild-type (WT) tobacco turned curl, yellow, and withered. Moreover, the roots were visibly black and rotten. In contrast, the PnWRKY15-OE tobacco lines kept healthy growth (Fig. 3B). In addition, after 7 days inoculation with F. solani, the WT tobacco leaves appeared with obvious yellowing and decay, whereas the leaves from four PnWRKY15-OE tobacco lines were only slightly yellow or normal (i.e., no decay) (Fig. 3C). The lesions caused by the F. solani infection were approximately 8-times larger on the WT leaves than that of PnWRKY15-OE leaves (Fig. 3D). Hence, ectopic expression of PnWRKY15 in tobacco significantly increased the resistance to F. solani.
The tobacco transcriptome profile was altered by the overexpression of PnWRKY15
To clarify the regulatory effect of PnWRKY15, one of the PnWRKY15-OE tobacco lines (15 - 2) was selected for a transcriptome sequencing (RNA-seq) analysis. Compared with the WT tobacco, 6,820 genes were differentially expressed in the PnWRKY15-OE tobacco. Of these differentially expressed genes (DEGs), 2,245 and 4,575 were up-regulated and down-regulated, respectively. The enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways among the DEGs are presented in Fig. 4A and Supplementary Material 1: Table S1. The most significantly enriched KEGG pathways were plant–pathogen interaction, plant hormone signal transduction, phenylpropanoid biosynthesis, and photosynthesis (Fig. 4B–E). The expression of several unigenes involved in plant-pathogen interactions was induced in the PnWRKY15-OE tobacco line (Fig. 4B). The expression levels of key genes related to JA biosynthesis, including genes encoding allene oxide synthase (gene_80914, gene_80912, gene_43960, and gene_53065), were much higher in the PnWRKY15-OE tobacco than that of WT tobacco (Fig. 4C, Supplementary Material 1: Table S2). In contrast, Jasmonate ZIM-domain genes (gene_6599, gene_24945, and gene_48999), which are associated with the repression of JA signal transduction, were expressed at lower levels in the PnWRKY15-OE tobacco than that of the WT tobacco. Moreover, the expression levels of the TGA genes (gene_76084, gene_1987, and gene_65035), which encode TFs that regulate SA signaling, were clearly up-regulated in the PnWRKY15-OE tobacco (Fig. 4C). Additionally, the expression of genes related to phenylpropanoid biosynthesis differed significantly between the WT and PnWRKY15-OE tobacco samples, including genes encoding phenylalanine deaminase (gene_62123), cinnamyl alcohol dehydrogenase (gene_3217), and peroxidase (gene_47769, gene_14429, gene_29358, gene_58827, and gene_55552) (Fig. 4D). Furthermore, multiple photosynthesis-related genes had down-regulated expression levels (Fig. 4E). These results indicated that ectopic expression of PnWRKY15 had a wide regulation on tobacco gene expression.
Overexpression of PnWRKY15 in tobacco activated the JA/SA signaling pathways and plant–pathogen interactions
The RNA-seq analysis revealed the extensive changes in the transcriptome of the PnWRKY15-OE tobacco. Therefore, the relative expression levels of many genes associated with JA/SA signaling pathways and plant-pathogen interactions were analyzed by qRT-PCR. Four PnWRKY15-OE tobacco lines (15 - 2/-21/-22/-24) were included in this experiment (Fig. 5). Five JA biosynthesis-related genes (NtAOC, NtPACX, NtAOS, NtJMT, and NtOPR) were expressed at higher levels in the three PnWRKY15-OE tobacco lines than that of WT tobacco, as was NtMYC, which encodes a TF that positively regulates JA signaling. Some PR genes, such as NtGLU1, NtPR1, Ntosmotin, and NtCHI, were significantly more highly expressed in the PnWRKY15-OE tobacco lines than that of WT. Of these genes, NtPR1 is a marker gene of the SA signaling pathway .
On the basis of above gene expression analysis, JA and SA contents in the PnWRKY15-OE tobacco were measured (Fig. 5). The average SA/JA contents of PnWRKY15-OE tobacco were 2.1-times and 7.5-times higher than that of the WT tobacco, respectively. The significant increases in the JA and SA contents in the PnWRKY15-OE tobacco indicated that PnWRKY15 may activate the JA and SA signaling pathways in tobacco, both of which were reportedly important pathways in plant disease resistance.
PnWRKY15 trans-activated an F. solani resistance-related gene, PnOLP1
Down-regulation of PnWRKY15 through RNAi significantly suppressed PnOLP1 expression (Fig. 2B). Moreover, overexpression of PnWRKY15 induced the expression of PR genes in tobacco, including Ntosmotin (Fig. 5). We previously identified PnOLP1 as an F. solani resistance-related gene . It was reasonable to verify whether PnWRKY15 had a direct regulation on PnOLP1 transcription. Therefore, the PnOLP1 promoter (PPnOLP1) was cloned to determine whether PnWRKY15 can directly bind to PPnOLP1. The PPnOLP1 sequence (965 bp) is shown in Supplementary Material 2. It has a W-box (TTGACC) element (− 31 to − 37 bp) (Supplementary Material 1: Table S3), as well as IAA- and ABA-responsive elements, high-salt- and dark-responsive elements, and a MYB-binding site.
The PnWRKY15-His recombinant protein was expressed in Escherichia coli and purified. The SDS-PAGE analysis indicated that the PnWRKY15-His fusion protein was approximately 36.7 kDa (Supplementary Material 3: Fig. S1 and S2). Additionally, the PPnOLP1 W-box sequence was synthesized and labeled with biotin. Electrophoretic mobility shift assay (EMSA) was conducted and it provided evidence of interaction between PnWRKY15 and the W-box of PPnOLP1 in vitro (Fig. 6A). The delayed migration of the band in lane 2 of the gel reflected the binding of the PnWRKY15-His recombinant protein to the W-box probes to form a complex with a higher molecular weight than PnWRKY15-His. The opposite result was observed in lane 4 and there were no bands that migrated more slowly in the gel than the biotin labeled free probes when the W-box was mutated.
Whether PnWRKY15 can activate the transcription of PnOLP1 was assessed by conducting a yeast one-hybrid (Y1H) assay (Fig. 6B). The yeast cells with pAbAi-PPnOLP1/pGADT7-PnWRKY15 were added to SD/−Leu solid medium containing 200 ng/mL ABA. These cells grew similarly to the positive control cells (i.e., Y1Hgold cells containing pAbAi-p53/pGADT7-P53). Conversely, the negative control yeast cells containing pAbAi-PPnOLP1/pGADT7 were unable to survive on the selection medium. These results indicated that PnWRKY15 can activate PnOLP1 transcription in yeast.
PnOLP1 expression was up-regulated by PnWRKY15
On the basis of the EMSA and Y1H assay results, the PPnOLP1-GUS construct was inserted into tobacco to determine the regulatory effects of PnWRKY15 on PnOLP1 transcription in vivo. Firstly, the PPnOLP1 activity was evaluated by measuring the β-glucuronidase (GUS) activity of the transgenic tobacco carrying PPnOLP1-GUS. Three randomly selected PPnOLP1-GUS tobacco lines were treated with plant hormones, including MeJA, SA, ABA, and gibberellic acid (GA) (Fig. 7A). The GUS activity increased significantly after the MeJA treatment. More specifically, the average GUS activity after the MeJA treatment was approximately 2-times higher than the untreated control. In addition, the GUS activity in the PPnOLP1-GUS tobacco lines treated with ABA and SA was 27–33 pmol 4-methylumbelliferone (4-MU) min− 1 µg− 1 protein, which was higher than the control activity (18.9–20.4 pmol 4-MU min− 1 µg− 1 protein), implying that both SA and ABA can activate PPnOLP1 transcription. Thus, PPnOLP1 is an inducible promoter that is responsive to plant hormones.
The GUS activity was also measured in the PPnOLP1-GUS/PnWRKY15-OE tobacco lines (Fig. 7B). There was no significant difference in the GUS activity between the pBI121-35S-GUS/PnWRKY15 tobacco and the pBI121-35S-GUS tobacco, indicating PnWRKY15 does not interact with the CaMV 35S promoter. GUS activity was reduced when it was fused with PPnOLP1 promoter, however, when it was co-expressed with PnWRKY15, the GUS activity (approximately 40 pmol 4-MU min− 1 µg− 1 protein) was about 2-times of that from the PPnOLP1-GUS tobacco. These results indicated that PnWRKY15 can activate the transcription of PnOLP1.
The WRKY family is one of the largest TF families in plants, with important roles in immune responses to pathogens. Specifically, WRKY TFs can directly interact with their target genes to activate or inhibit plant defense activities. Moreover, WRKY TFs, mitogen-activated protein kinases, and hormone signaling pathways cooperatively regulate immune responses . In this study, a P. notoginseng WRKY TF (WRKY15) was identified and functionally characterized in terms of its contribution to the plant response to root rot. The subcellular localization experiment indicated PnWRKY15 is a nuclear protein. The down-regulated expression of PnWRKY15 via RNAi in P. notoginseng leaves increased the susceptibility to root rot pathogen F. solani. In contrast, ectopic expression of PnWRKY15 in transgenic tobacco conferred a high level of resistance to F. solani. Thus, PnWRKY15 positively regulates P. notoginseng resistance to root rot, similar to other WRKY TFs. For example, overexpression of the Jatropha curcas WRKY2 gene in tobacco reportedly increases the resistance to Macrophomina phaseolina . The virus-induced silencing of the Paeonia lactiflora WRKY65 gene increases the susceptibility of plants to Alternaria tenuissima .
In response to biotic and abiotic stresses, multiple TFs, including WRKY TFs, are activated to modify the expression of defense-related genes (i.e., transcriptome reprogramming). In apple, WRKY46 enhances the resistance to B. dothidea by binding to the W-box element in the MdPBS3.1 promoter and up-regulating MdPBS3.1 expression . In wild lily (Lilium regale Wilson) infected with F. oxysporum, WRKY1 positively modulates the expression of the resistance gene LrPR10-5, leading to strong Fusarium-wilt resistance . In the current study, the expression levels of three disease resistance-related genes (Ntosmotin, NtGLU1, and NtCHI) were up-regulated in the PnWRKY15-OE tobacco lines. Additionally, PnOLP1 expression was induced by MeJA and SA. An earlier study showed PnOLP1 is involved in the defense response to F. solani . The PnOLP1 recombinant protein expressed in E. coli has antifungal effects on several phytopathogens, including F. solani. Moreover, PnOLP1-OE tobacco lines exhibit increased resistance to F. solani. The EMSA and Y1H assay results revealed that PnWRKY15 directly and specifically binds to the PnOLP1 promoter and trans-activates PnOLP1. The subsequent co-expression of PnOLP1 promoter and PnWRKY15 in tobacco indicated that PnWRKY15 can activate the transcription of PnOLP1. These findings of this study suggest PnWRKY15 alters PnOLP1 transcription to enhance the P. notoginseng defense response to root rot.
In addition to modulating the expression of many disease resistance-related genes, WRKY TFs are involved in the cross-talk between plant hormone signaling pathways under stress conditions. In cotton, GhWRKY70 has a major role in the regulation of the JA and SA signaling pathways in response to Verticillium dahliae infections . More specifically, it negatively regulates the resistance to V. dahliae by activating the expression of GhNPR1 and GhPR1, but inhibiting the expression of GhPDF1.2 and GhPR3. In tomato (Solanum lycopersicum), the overexpression of WRKY46 represses the expression of JA and SA signaling pathway marker genes, PR1, and protease inhibitor genes (PI I and PI II), which increases the susceptibility to B. cinerea . In the present study, we completed an in-depth investigation of the mechanism underlying the regulatory effects of PnWRKY15 on P. notoginseng resistance to F. solani. The RNA-seq analysis showed that SA and JA signaling pathways were more active in the PnWRKY15-OE tobacco than that in the WT tobacco. The qRT-PCR data and hormone contents confirmed that JA/SA biosynthesis and signaling were induced by the ectopic expression of PnWRKY15 in tobacco. Therefore, the positive regulatory effects of PnWRKY15 on JA and SA signaling pathways are important for activating the P. notoginseng defense response to F. solani (Fig. 8).
The network regulating plant immune responses, which includes WRKY TFs, is highly complex. The synergistic effects of P. notoginseng WRKY9 and the JA signaling pathway on the expression of an antimicrobial peptide gene (PnDef1) in response to F. solani have been reported . Ocimum sanctum WRKY1 positively regulates the phenylpropanoid pathway, resulting in increased rosmarinic acid contents in transgenic A. thaliana and enhanced resistance to Pseudomonas syringae pv. tomato . The ectopic expression of WRKY31, which was identified in an Erysiphe necator-resistant grape (Vitis quinquangularis), protects Vitis vinifera from E. necator by promoting SA signaling and inducing stilbene biosynthesis . Interestingly, compared with the WT tobacco, the phenylpropanoid pathway was more active in the PnWRKY15-OE tobacco, but photosynthetic activities were substantially suppressed. Photosynthesis is essential for normal plant growth and development, but it is affected when plants are subjected to biotic and abiotic stresses . Future studies should explore whether PnWRKY15 promotes phenylpropanoid accumulation and regulates the dynamic equilibrium between growth and defense responses following an infection by F. solani. Furthermore, development of a genetic transformation system for P. notoginseng will enable researchers to comprehensively characterize the mechanism by which WRKY TFs regulate the P. notoginseng defense response to root rot.
In this study, silencing of PnWRKY15 increased the sensitivity of P. notoginseng to the root rot pathogen F. solani, whereas its ectopic expression in tobacco increased the resistance to F. solani. The PnWRKY15 protein was localized in the nucleus and activated the transcription of the F. solani resistance-related gene PnOLP1 by binding to the W-box element in the promoter. The PnWRKY15 TF positively modulated PnOLP1 expression and induced the SA and JA signaling pathways as well, during the defense response to F. solani. The data generated in this study indicate that PnWRKY15 is a TF that enhances root rot resistance by up-regulating PnOLP1 expression and inducing JA/SA signaling pathways.
Materials and methods
Plant and fungal materials
The 1-year-old P. notoginseng plants and wild-type tobacco (Nicotiana tabacum L.) seeds were provided by Dr. Guanze Liu from Yunnan Agricultural University, Kunming, China. The young leaves of P. notoginseng plants grown under a shade net were used for the cloning of PnWRKY15 and PPnOLP1 as well as the RNAi experiment. Fusarium solani was preserved in our laboratory and activated on potato dextrose agar medium before use. The wild-type tobacco seeds were surface-sterilized in 75% alcohol and a 0.1% HgCl2 solution before they were sown on quarter-strength Murashige and Skoog (MS) medium. Two-month-old seedlings were used for the genetic transformation.
Cloning of PnWRKY15
Gene-specific primers (Supplementary Material 1: Table S4) were designed on the basis of the PnWRKY15 cDNA sequence obtained from  to amplify the PnWRKY15 ORF via RT-PCR using the Eastep® RT Master Mix kit (Promega, USA). The PCR product was sequenced after T-A cloning to verify that the PnWRKY15 sequence was correct. The conserved domain in the PnWRKY15 amino acid sequence was used to search the NCBI database (https://www.ncbi.nlm.nih.gov/guide/homology/). The PnWRKY15 amino acid sequence homology was analyzed using the tblast online tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi).
The PnWRKY15 ORF was ligated into the pBINm-gfp5 vector digested with BamHI and XbaI to generate the PnWRKY15-GFP construct (Supplementary Material 1: Table S4). The recombinant vector was transferred into Agrobacterium tumefaciens EHA105 cells using liquid nitrogen (i.e., quick freezing-based method). Onion epidermal cells were transformed with A. tumefaciens containing pBINm-gfp5-PnWRKY15 or the pBINm-gfp5 empty vector and then cultured on MS medium for 2 days in darkness before examining the localization of green fluorescence using a laser scanning confocal microscope (Nikon, Japan). The red fluorescence of propidium iodide was observed to indicate the nucleus in the onion epidermal cells.
Gene-specific primers with attB linkers were designed (Supplementary Material 1: Table S4) for the PCR amplification of a PnWRKY15 fragment (420 bp) fused with attB linkers. The pHellsgate 2-PnWRKY15 vector was constructed using the Gateway BP Clonase™ II Enzyme Mix kit (Invitrogen, USA) and was then transformed into A. tumefaciens EHA105 cells. Young leaves from 1-year-old P. notoginseng plants grown under normal conditions were wounded using a sterile syringe and then divided into two groups. The A. tumefaciens cells containing pHellsgate 2-PnWRKY15 were added to the wounded leaves in one group, whereas the A. tumefaciens cells containing the pHellsgate 2 empty vector were added to the wounded leaves in the other group. The leaves in both groups were placed on moistened sterile filter paper and incubated for 24 h at 25 °C in darkness. All of the leaves were inoculated with F. solani spore suspensions and incubated for 72 h under light. The disease symptoms were recorded using a digital camera (Nikon). The lesion areas were calculated using Adobe Photoshop 2020. The PnWRKY15, PnAOS, PnPR-1, PnMYC2, PnCHI, and PnOLP1 expression levels in the leaves were analyzed by qRT-PCR using gene-specific primers (Supplementary Material 1: Table S5).
Development of PnWRKY15-OE tobacco lines and analysis of disease resistance
The pCAMBIA2300S-PnWRKY15 vector was constructed as described above (primer sequences are listed in Supplementary Material 1: Table S4) and then transferred into WT tobacco leaf discs via A. tumefaciens LBA4404. The leaf discs were induced to produce adventitious buds in MS medium containing kanamycin, 1-naphthylacetic acid, and cytokinin. The buds were transferred to half-strength MS medium containing kanamycin to induce rooting under light (26 °C, 16-h light:8-h dark cycle). Genomic DNA was extracted from the regenerated tobacco seedlings using cetyltrimethylammonium bromide (CTAB) to identify transgenic plants. The confirmed transgenic seedlings were transferred to the greenhouse. The T2 lines used in the subsequent experiments were obtained through self-pollination.
Selected T2PnWRKY15-OE tobacco lines were used for a qRT-PCR analysis of PnWRKY15 expression levels using the Eastep® qPCR Master Mix kit (Promega), with WT tobacco serving as the negative control (primer sequences are listed in Supplementary Material 1: Table S5). The tobacco Ntactin gene (AB158612.1) was chosen as the internal reference to calculate relative gene expression levels according to the 2−ΔΔCt method. This experiment included three biological replicates. The generated data were analyzed by performing t-tests.
The four PnWRKY15-OE tobacco lines with high PnWRKY15 expression levels were analyzed regarding their resistance to F. solani. The F. solani spore suspension was prepared after activating the fungus on potato dextrose agar medium. Young tobacco leaves were harvested and wounded using a sterile pipette tip before they were inoculated with the spore suspension. The inoculated leaves were placed on moistened filter paper and incubated for 7 days under light (26 °C, 16-h light:8-h dark cycle). Tobacco roots were immersed in an F. solani spore suspension for 30 min. The inoculated tobacco plants were grown under hydroponic conditions in an illumination incubator for 7 days. The disease symptoms were recorded using a digital camera and the lesion areas were calculated.
Transcriptome sequencing analysis of the PnWRKY15-OE tobacco
Total RNA was extracted from the young leaves of WT and PnWRKY15-OE tobacco (line 15 - 2) plants grown under normal conditions. After determination of the RNA concentration, purity, and integrity using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA) and the Agilent Bioanalyzer 2100 (Agilent Technologies Inc., USA), the high-quality RNA was used to construct the sequencing library, which was analyzed using the Illumina NovaSeq 6000 sequencing platform. The RNA-seq analysis was performed by Shanghai Majorbio Bio-pharm Technology Co., Ltd. The transcriptome quality assessment, transcriptome assembly, analysis of the correlation between samples, analysis of the DEGs, gene ontology functional annotation, and KEGG pathway analysis [30,31,32] were performed using the Majorbio Cloud Platform (www.majorbio.com). The RNA-seq experiment involved three biological replicates.
To verify the DEGs identified by the RNA-seq analysis, the relative expression levels of some genes related to JA biosynthesis and JA/SA signal transduction as well as a few PR genes were determined by qRT-PCR. This analysis was completed using line 15 - 2 (i.e., the line used for the RNA-seq analysis) and two randomly selected tobacco lines (15-21/-24). The qRT-PCR assay was performed as described above using gene-specific primers (Supplementary Material 1: Table S5).
Hormone contents determination of PnWRKY15-OE tobacco lines
An ultra-high performance liquid chromatography-mass spectrometry system was used to measure the JA and SA contents in the PnWRKY15-OE tobacco lines (15 − 2/-21/-24) and the WT tobacco as previously described . The chromatographic peak area for the JA or SA in each sample and the linear equation of the standard curve were used to calculate the corresponding concentrations in the tested samples.
Cloning of PPnOLP1
Genomic DNA was extracted from young P. notoginseng leaves using the CTAB method. Two nested PCR primers (PnOLP1-GSP1 and PnOLP1-GSP2, Supplementary Material 1: Table S4) were designed to amplify the promoter of PnOLP1, which encodes an osmotin-like protein that confers resistance to F. solani . The PPnOLP1 sequence was cloned according to the Universal GenomeWalker 2.0 user manual (Takara, Japan). The cis-elements in PPnOLP1 were identified using PLANTCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/).
Gene-specific primers were designed and used to amplify PnWRKY15 (without the stop codon) (Supplementary Material 1: Table S4). The pET32a-PnWRKY15 recombinant vector was constructed using the ClonExpress II One Step Cloning Kit (Vazyme, China) and then inserted into E. coli BL21 (DE3) cells (Tsingke Biotechnology, China). The expression of the recombinant PnWRKY15 protein was induced by adding 2 mM isopropyl-β-D-1-thiogalactoside to the bacterial solution and incubating at 25 °C for 8 h. The protein was denatured and purified as previously described . The PPnOLP1 sequence was used to design the following probes containing the W-box element: biotin labeled probe, unlabeled competitor probe, and biotin labeled mutant probe (i.e., mutated W-box) (Supplementary Material 1: Table S4). The probes were synthesized and labeled with biotin (Sangon Biotech, China), except for the unlabeled competitor probe. The EMSA was performed using the LightShift™ Chemiluminescent EMSA kit (Pierce, USA).
A pair of gene-specific primers (Supplementary Material 1: Table S5) was designed to construct the pAbAi-PPnOLP1 decoy vector (Clontech, USA). The pAbAi-PPnOLP1 recombinant vector was incorporated into Y1Hgold yeast cells, which were then grown on SD/−Ura medium. The pGADT7-PnWRKY15 prey vector (Clontech) was constructed after the PnWRKY15 ORF was amplified by PCR using gene-specific primers (Supplementary Material 1: Table S4). The prey vector was inserted into Y1Hgold yeast cells, which were grown on SD/−Leu medium. The pAbAi-p53 decoy vector and the pGADT7-P53 prey vector from the Matchmaker® Gold Yeast One-Hybrid Library Screening System Kit (Clontech) were used as positive controls. The Y1Hgold yeast cells carrying pAbAi-p53/pGADT7-P53 (positive control), pAbAi-PPnOLP1/pGADT7 AD (negative control), and pAbAi-PPnOLP1/pGADT7-PnWRKY15 were added to plates containing SD/−Leu/AbA medium supplemented with 200 ng/mL ABA. The plates were incubated at 28 °C for 3 days to confirm whether PnWRKY15 can interact with PPnOLP1.
Analysis of GUS activity
The pBI121-PPnOLP1-GUS recombinant vector was constructed and then inserted into A. tumefaciens LBA4404 cells for the transformation of WT tobacco and PnWRKY15-OE tobacco. The tobacco shoots were induced in a tissue culture system. The PPnOLP1-GUS and PPnOLP1-GUS/PnWRKY15 transgenic tobacco plants were verified by PCR analysis. Leaf discs from three PPnOLP1-GUS transgenic tobacco plants were treated with sterile water (control), 100 µM MeJA, 100 µM SA, 100 µM ABA, or 100 µM GA solutions for 2 h. The GUS activity was measured using a fluorescence spectrophotometer as previously described . The GUS activity of the PPnOLP1-GUS/PnWRKY15 transgenic tobacco was determined using pBI121-GUS transgenic tobacco as the control. The GUS activity assay included three biological replicates.
The gene expression, lesion area, GUS activity, and JA/SA content data were analyzed using Excel 2019 and SPSS 23.0.
The original contributions presented in the study are publicly available. The PPnOLP1 data generated in this study have submitted to NCBI GenBank database (https://www.ncbi.nlm.nih.gov/, and the GenBank accession number is OP970657).
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We thank Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript.
This work was supported by the National Natural Science Foundation of China (grant number 82060693).
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All our experiments have complied with relevant institutional, national, and international guidelines and legislation.
Statement Plant materials of Panax notoginseng were collected from Yunnan Provincial Key Laboratory of Panax notoginseng, Kunming, Yunnan, China. Plant materials of Nicotiana tabacum were collected from Yunnan Agricultural University, Kunming, China and kindly provided by Professor Guanze Liu. The plant materials don’t include any wild species at risk of extinction. We comply with relevant institutional, national, and international guidelines and legislation for plant study.
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Su, L., Zheng, L., Wang, H. et al. Panax notoginseng transcription factor WRKY15 modulates resistance to Fusarium solani by up-regulating osmotin-like protein expression and inducing JA/SA signaling pathways. BMC Plant Biol 23, 362 (2023). https://doi.org/10.1186/s12870-023-04373-x