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Table 2 Comparison of phytochemical profile from TI sample obtained in February 2018 and extracted using cold versus hot water traditional extraction methods

From: Season, storage and extraction method impact on the phytochemical profile of Terminalia ivorensis

Phytochemical class

Phytochemical metabolites

Hot water (ng/mg)

Hot water relative abundance (%)

Cold water (ng/mg)

Cold water relative abundance (%)

Acetophenones

3,4,5-Trimethoxyacetophenone

1.8 ± 0.4

0.00

6.5 ± 1.8***

0.01

 

4-Hydroxy-3-methoxyacetophenone

6.8 ± 1.7

0.01

33.3 ± 8.2***

0.04

 

4-Hydroxyacetophenone

8.0 ± 2.0

0.01

80.4 ± 20.1***

0.09

Amines

Serotonin

9.1 ± 2.2

0.02

5.0 ± 1.2

0.01

 

Tyramine

0.7 ± 0.2

0.00

1.1 ± 0.4

0.00

Benzaldehydes

3,4-Dimethoxybenzaldehyde

10.0 ± 2.2

0.02

16.7 ± 4.1

0.02

 

P-Hydroxybenzaldehyde

5.8 ± 1.4

0.01

28 ± 6.9***

0.03

 

Protocatachaldehyde

133.2 ± 33.3

0.24

348.4 ± 87.1***

0.39

 

Syringin

10.8 ± 2.4

0.02

40.8 ± 9.8***

0.05

 

Vanillin

51.2 ± 12.8

0.09

155.6 ± 38.8***

0.18

Benzoic acids

Benzoic acid

107.6 ± 26.9

0.19

1480 ± 370***

1.67

 

Chlorogenic acid

10.4 ± 2.6

0.02

11.9 ± 2.9

0.01

 

Gallic acid

4040 ± 1010

7.30

9000 ± 2250***

10.15

 

P-Hydroxybenzoic acid

44.8 ± 11.2

0.08

137.2 ± 34.3***

0.15

 

Protocatechuic acid

524 ± 131

0.95

860 ± 215*

0.97

 

Salicylic acid

32.3 ± 8.0

0.06

56.4 ± 14.1

0.06

 

Syringic acid

36.9 ± 9.2

0.07

73.6 ± 18.4**

0.08

 

Vanillic acid

109.2 ± 27.2

0.20

254.8 ± 63.7**

0.29

Cinnamic acids

4-Methoxycinnamic acid

7.8 ± 1.9

0.01

7.7 ± 1.9

0.01

 

Caffeic acid

95.2 ± 23.8

0.17

328.4 ± 82.1***

0.37

 

Ferulic acid

131.6 ± 32.9

0.24

289.6 ± 72.4***

0.33

 

P-Coumaric acid

108.8 ± 27.2

0.20

226.4 ± 56.5**

0.26

 

Sinapic acid

1.0 ± 0.2

0.00

14.2 ± 3.5***

0.02

Flavonoids

Apigenin

25.2 ± 6.3

0.05

49.2 ± 12.3*

0.06

 

Biochanin A

16.3 ± 4.0

0.03

18.9 ± 4.7

0.02

 

Catechin

27,640 ± 6910

49.95

52,800 ± 13,200**

59.52

 

Epicatechin

2840 ± 710

5.13

1688 ± 422

1.90

 

Epigallocatechin

2128 ± 532

3.85

1808 ± 452

2.04

 

Epigallocatechin gallate

5320 ± 1330

9.61

5160 ± 1290

5.82

 

Formononetin

3.1 ± 0.7

0.01

3.2 ± 0.8

0.00

 

Gallocatechin

7680 ± 1920

13.88

9040 ± 2260

10.19

 

Genstein

28.6 ± 7.1

0.05

53.6 ± 13.4*

0.06

 

Hesperitin

5.9 ± 1.9

0.01

10.9 ± 2.7*

0.01

 

Kaempferol

89.2 ± 22.3

0.16

184.8 ± 46.2**

0.21

 

Luteolin

59.6 ± 14.9

0.11

79.6 ± 19.9

0.09

 

Myricetin

210 ± 52.5

0.38

186.4 ± 46.6

0.21

 

Naringenin

179.6 ± 44.9

0.32

285.2 ± 71.3*

0.32

 

Phloretin

225.2 ± 56.3

0.41

420 ± 105*

0.47

 

Phloridzin

91.2 ± 22.8

0.16

141.6 ± 35.4

0.16

 

Quercetin

170 ± 42.5

0.31

262.8 ± 65.7*

0.30

 

Quercetin-3-glucoside

83.6 ± 20.9

0.15

182.8 ± 45.7**

0.21

 

Resveratrol

41.6 ± 10.4

0.08

51.2 ± 12.8

0.06

 

Scopoletin

42 ± 10.5

0.08

114 ± 28.5***

0.13

 

Tangeretin

10.7 ± 2.6

0.02

4.4 ± 1.1*

0.00

 

Taxifolin

226.4 ± 56.6

0.41

432 ± 108**

0.49

Indoles

Indole

24.2 ± 16.1

0.04

110.4 ± 27.6***

0.12

 

Indole-3-acetic acid

6.2 ± 1.5

0.01

56.4 ± 14.1***

0.06

Lignans

Indole-3-carboxaldehyde

19.6 ± 4.9

0.04

83.6 ± 20.9***

0.09

 

Pinoresinol

35.0 ± 8.7

0.06

35.1 ± 8.7

0.04

 

Secoisolariciresinol

151.6 ± 37.9

0.27

247.2 ± 61.8

0.28

 

Syringaresinol

1312 ± 329

2.37

916 ± 229

1.03

Phenols

Hydroxytyrosol

64.4 ± 16.1

0.12

108.4 ± 27.1**

0.12

Phenylacetic acids

Phenylacetic acids

14.9 ± 3.7

0.03

45.6 ± 11.4***

0.05

Phenyllactic acids

Phenyllactic acid

1.7 ± 0.4

0.00

4.2 ± 1.0*

0.00

Phenylpyruvic acids

4-hydroxyphenylpyruvic acid

1100 ± 275

1.99

664 ± 166**

0.75

 

SUM

55331.8

88703.5

100

100

  1. Data are presented as mean amount ± SD (ng/mg) and mean relative abundances (%), n = 3, *p < 0.05, **p < 0.01 and ***p < 0.001 as compared to hot water extraction of TI sample (obtained in dry season) by ANOVA followed by Bonferroni’s post hoc test