- Research
- Open access
- Published:
Jasmonic acid contributes to rice resistance against Magnaporthe oryzae
BMC Plant Biology volume 22, Article number: 601 (2022)
Abstract
Background
The annual yield losses caused by the Rice Blast Fungus, Magnaporthe oryzae, range to the equivalent for feeding 60 million people. To ward off infection by this fungus, rice has evolved a generic basal immunity (so called compatible interaction), which acts in concert with strain-specific defence (so-called incompatible interaction). The plant-defence hormone jasmonic acid (JA) promotes the resistance to M. oryzae, but the underlying mechanisms remain elusive. To get more insight into this open question, we employ the JA-deficient mutants, cpm2 and hebiba, and dissect the JA-dependent defence signalling in rice for both, compatible and incompatible interactions.
Results
We observe that both JA-deficient mutants are more susceptible to M. oryzae as compared to their wild-type background, which holds true for both types of interactions as verified by cytological staining. Secondly, we observe that transcripts for JA biosynthesis (OsAOS2 and OsOPR7), JA signalling (OsJAZ8, OsJAZ9, OsJAZ11 and OsJAZ13), JA-dependent phytoalexin synthesis (OsNOMT), and JA-regulated defence-related genes, such as OsBBTI2 and OsPR1a, accumulate after fungal infection in a pattern that correlates with the amplitude of resistance. Thirdly, induction of defence transcripts is weaker during compatible interaction.
Conclusion
The study demonstrates the pivotal role of JA in basal immunity of rice in the resistance to M. oryzae in both, compatible and incompatible interactions.
Background
Rice is the staple food for more than half of the world population, but drastically affected by the Rice Blast Fungus, Magnaporthe oryzae. The yield loss is estimated to range up to 30% of the total rice production [1], and thus poses major threats to global food security. This ascomycete pursues a hemi-biotrophic lifestyle, feeding on the living tissue to obtain nutrients during an early biotrophic stage, subsequently switching to necrotrophic growth, killing the host cells by toxins and degrading enzymes. The infection cycle begins with spore attachment to the leaf surface, where spores germinate to develop a specialised penetration structure, the appressorium. The appressorium can develop, by means of osmolyte accumulation, considerable pressure to rupture cuticle and cell wall. After penetration, invasive hyphae form to acquire nutrients from the host cell, while effectors silence the immunity of the host. After 1 week of colonisation, the pathogen sporulates, such that spores can spread to neighbouring plants to initiate a new infection cycle.
In response to pathogen infection, plants have evolved a variety of strategies to protect themselves from invasion by pathogens. The first tier is formed by preformed defence, including constitutive physical or chemical barriers, such as cuticles [2], constitutively produced phytoalexins, called phytoanticipins [3, 4], or constitutive expression of defence genes [5]. Generally, preformed defence is not specific for a certain pathogen, but rather confers a broad and durable resistance to a variety of pathogens [5]. In addition to this preformed defence, plants also can rely on induced defence. The first layer of induced defence is so called pathogen-associated molecular pattern triggered immunity (PTI), activated by conserved molecular features of microbial pathogens, termed pathogen- or microbe-associated molecular patterns (PAMPs or MAMPs). For instance, detection of flagellin allows to sense most bacterial invaders, while detection of chitin allows detection of fungal attacks [6,7,8]. In consequence of co-evolutionary adaptation to certain pathogens, this basal layer of plant immunity can be silenced by effectors from specialised pathogens, which in turn, can activate a second layer of induced defence, called effector-triggered immunity (ETI), which is often, but not always, accompanied by hypersensitive cell death of the infected cell [9].
The complexity of innate immunity requires extensive signalling to deploy responses appropriate for the respective defence context. Plant hormones are therefore key regulators of plant immunity [10]. The biosynthesis of JA starts from the release of linolenic (18:2) and α-linolenic (18:3) acid from the chloroplast membrane, providing substrates for the 9-lipoxygenases (9-LOX) and 13-lipoxygenases (13-LOX) which catalyse the formation of 9S-hydroxyoctadecadienoic acid (HPODE) and 13S-HPODE [11]. In rice, the HPODEs are then converted by allene oxide synthase (OsAOS1 and OsAOS2), and allene oxide cyclase (OsAOC) into 12-oxo phytodienoic acid (12-OPDA) [12, 13]. Subsequently, OPDA is shipped out of the chloroplast by a channel protein JASSY to peroxisomes [14]. Following reduction by 12-oxo phytodienoic acid reductase (OsOPR7) and 3 sequential steps of β-oxidation, OPDA is transformed into JA [15]. JA is further activated via the GH3 amido synthetase OsJAR1 by conjugation of JA to the amino acid isoleucine, leading to the formation of JA-Ile [16, 17]. JA-Ile can bind to the receptor complex SCFCOI1 to direct the degradation of JAZ proteins by way of the 26S-proteasome pathway, which can derepress downstream transcription factors, such as OsMyc2, and subsequent activation of JA-dependent gene expression [18, 19]. Among the 15 JAZ-encoding genes in rice, almost all of them are stress-responsive, with 9 of them particularly responsive to mechanical wounding [20].
JA and its derivatives, collectively termed as jasmonates, are reported to be involved in wounding, regulation of secondary metabolism, and the responses to abiotic and biotic stress. In dicots, jasmonates are generally responsible for defence against necrotrophic pathogens, while salicylic acid is more important for resistance against biotrophic pathogens. There exists a pronounced antagonism between jasmonate-dependent and the SA-dependent pathways, confirmed for 17 plant species so far and, thus, representing an evolutionary ancient acquisition [21]. However, the situation seems different for rice, because, here, JA and SA signalling are synergistic in most cases, although a few examples for mutual antagonism have been reported as well [22, 23]. For example, more than half of genes in rice activated by SA analog benzothiadiazole can also be induced JA, while a third of them is repressed, thus responding inversely. In rice, jasmonates has been implied to contribute to resistance against various pathogens with different lifestyles, including the hemibiotrophic pathogens M. oryzae and Xanthomonas oryzae, the necrotrophic pathogen Rhizoctonia solani, and the biotrophic root knot nematode Meloidogyne graminicola [24,25,26].
With respect to M. oryzae, previous studies have shown a role of jasmonates for different events of the the response to virulent strains. Not only can jasmonates induce the accumulation of reactive oxygen species, but they also modulate diterpenoid and flavonoid phytoalexin production after elicitation by, for instance, CuCl2 and chitin [27,28,29]. Overexpression of OsAOS2 enhanced the accumulation of jasmonates and transcripts for pathogenesis-related (PR) gene expression during infection with the M. oryzae [12]. Conversely, the JA biosynthesis deletion mutants osjar1 (a OsJAR1 retrotransposon insertion mutant), cpm2, and hebiba (OsAOC deletion mutants) displayed a higher susceptibility to the this fungus, accompanied by reduced accumulation of flavonoid phytoalexins, such as sakuranetin, while the production of diterpenoid phytoalexins was not affected [13, 30]. These findings suggest a scenario, where JA can enhance rice basal defence against M. oryzae through promoting oxidative burst, induction of PR genes, and accumulation of the flavonoid phytoalexin sakuranetin [12, 13, 27]. However, data where jasmonate synthesis seems to be dispensable or even negatively correlated with defence against M. oryzae challenge this hypothesis. For example, the OsF78i mutant affected in the expression two omega-3 fatty acid desaturases, OsFAD7 and OsFAD8, leading to marked reduction in 18:3 linolenic acid and, as a consequence, to impaired wounding-induced JA accumulation, is not more susceptible, but more resistant to M. oryzae [31]. Likewise, two JA knock-down mutants, AOCRi and OPRRi, deficient in JA accumulation, develop disease symptoms to the same extent as wild type plants during both, compatible and incompatible, interactions, suggesting that JA is dispensable for the defence against M. oryzae [32]. Even worse, a recent finding that M. oryzae produces jasmonates by itself to promote appressorium formation challenges the role of JA as promoting factor against M. oryzae [33].
We have previously identified two JA-deficient mutants, cpm2 and hebiba, lacking a functional gene for Allene Oxide Cyclase (AOC) which allowed to address the role of host-derived JA for the infection process [13]. Here, we saw a difference between compatible interactions (where the mutants behaved similar to the wildtype) and incompatible interactions (where the mutants were more susceptible). These findings, using detached leaf sheaths as experimental model, indicate a possible role for JA for the hypersensitive response, as characteristic feature of incompatible interactions. There exists considerable controversy with respect to the role of JA for the hypersensitive reaction, with both, positive [34,35,36,37], and negative [38, 39] interactions being proposed. This might be partially due to differences in experimental models and conditions. The conclusions from our previous analysis is limited by the fact that excised leaf sheaths were used rather than entire living plants. Furthermore, the virulent and avirulent M. oryzae strains used in that study differed with respect to their genetic background. Thus, the role of jasmonates in resistance to M. oryzae has remained inconclusive, contrasting with the situation for bacterial blight resistance [40, 41], spikelet development [42] and drought stress [43], their function in rice blast fungus defence, where JAZ genes have been shown to be involved. A second open question is the interaction of JA and SA in this context [22, 44, 45].
To address these limitations of our previous study, we now used isogenic strains of M. oryzae carrying the cognate avirulence/resistance genes avrPia/Pia, and we conducted these studies in intact plants to more specifically explore and complete our picture on the role of JA in the interaction between rice and the pathogenic fungus M. oryzae. To get insight into the interaction between JA and SA signalling, we monitored not only responses jasmonate synthesis and signalling genes, but also of genes that are known to be SA responsive. We test the hypothesis that, first, JA confers defence response in both compatible and incompatible interactions, and, second, that JA-dependent defence is paralleled by a second pathway not depending on JA, but possibly linked with SA signalling during both types of interactions. To this end, we used a factorial design with two isogenic strains GY11-EV (compatible) and GY11-AvrPia (incompatible) in combination with three genotypes (Nihonmasari wildtype, cpm2 and hebiba) to examine the role of JA in the defence to M. oryzae, through physiological, histological, and molecular approaches.
Material and methods
Plant material and growth condition
In this study, we compared three rice genotypes (Nihonmasari WT, cpm2 and hebiba) that were a collection of plant materials in our lab and generated as described previously [13]. The mutants cpm2 and hebiba are JA-deficient mutants arose by γ-irradiation in the background of the O. sativa ssp. japonica cultivar Nihonmasari and are affected in the synthesis of jasmonates due to deletions in the gene for allene oxide cyclase (AOC). The cpm2 mutant harbours a 11-bp deletion in exon 1 of AOC, while in the hebiba mutant, a large deletion of 170 kb eliminated not only the entire AOC, but also neighbouring genes [13]. Since homozygous mutants are male sterile, we had to maintain the lines through the heterozygotes, from which we were able to select homozygous plants due to their growth phenotype (the coleoptiles show an altered light response and therefore are long under irradiation, while the heterozygotes or the wild type seedlings show short coleoptiles). Seeds of good quality from each genotype were selected and surface-sterilised with 80% ethanol for 1 min, and then rinsed two times with sterile water, followed by soaking in 5% sodium hypochlorite for 20 min. We removed hypochlorite residues by rinsing the seeds with sterile water three times. The sterilised seeds were sown onto 0.45% phytoagar gel filled in Magenta boxes and allowed to germinate under a 12 h: 12 h light: dark cycle (photosynthesis active radiation (PAR) 120 μmol m− 2 s− 1). We selected seedlings displaying the phenotype (long coleoptiles) as homozygous mutants. The selected mutants and seedlings displaying the wild-type phenotype were subsequently transplanted to pots filled with compost (7/8 Neuhaus compost no. 9, and 1/8 Pozzolana, Neuhaus, France). The soil was humidified every day and fertilised once weekly (1.5 g/L NPK (17–7-22), and 0.25 g/L QUELARTAL Fe (6% w/v, Artal, Valencia). After further 14 days of growth in the greenhouse (20 °C during the night, and 30 °C during daytime), the seedlings had reached the four-leaf stage and were ready for inoculation.
Cultivation of Magnaporthe strains and infection assay
We employed two genetically engineered strains of Magnaporthe oryzae for the current study [46]: strain GY11-EV, harbouring an empty vector was virulent for the rice cultivar Nihonmasari, while the engineered strain-GY11-AvrPia expressed the avirulence gene AvrPia, and consequently was avirulent on Nihonmasari. Both strains were cultivated on rice flour medium and fungal spores harvested [47]. We adjusted the concentration of the suspension to 5 × 104 spores/ml with 0.5% gelatine for inoculation. Following inoculation, the plants were incubated in a dew chamber (25 °C, 100% humidity) in darkness for 16–18 h, and then transferred to a growth chamber (12 h in darkness at 25 °C and 12 h in fluorescent light at 30 °C, photosynthetically available radiation 120 μmol m− 2 s− 1) for the development of the symptoms. At days 1, 2 and 3 post inoculation (dpi), we sampled the fourth leaf of each plant for histological analysis or RNA extraction. At 7 dpi, when symptoms had fully appeared, infected leaves were excised, pasted onto a sheet of scale paper, and scanned digitally. The images were then subsequently used for quantitative image analysis using ImageJ (imagej.nih.gov/ij), scoring lesion areas (Analyze/Tool/ROI Manager), lesion number (Plugins/Analyze/Cell counter), and total leaf area (Analyze/Tool/ROI Manager). The total coverage of lesion areas, as well as the number of lesions were then normalised to total leaf area.
Histological analysis of fungal growth in rice leaves
Infected leaves were sampled at 2 and 3 dpi by cutting the fourth leaf into approximately 4 cm long segments, followed by fixation in 75% (v/v) ethanol, 25% (v/v) chloroform, and 0.15% (v/v) acetic acid for 2 days until the chlorophyll was fully removed. Subsequently, we cleared the tissue by rinsing with distilled water twice for 15 min, and then macerating with 0.05 M NaOH at 90 °C in a water bath for 15 min. Subsequently, we washed away NaOH with distilled water twice for 10 min, followed by buffering the tissue with Tris-HCl (0.1 M, pH 5.8) at 90 °C in a water bath for 30 min. After washing out the buffer with distilled water for 10 min, the tissue was pre-equilibrated with sterilized Phosphate Buffered Saline (PBS, pH 7.4) for 15 min at 20 °C, before staining with 0.002% WGA-Alexa Fluor 488 (Invitrogen, USA) in PBS overnight at 20 °C in darkness. We kept the stained tissue in sterilised 50% (v/v) glycerol at 4 °C until microscopic observation. Fungal hyphae in rice leaf tissue were observed under an epifluorescence microscope (AxioImager Z.1 microscope, Zeiss, Jena) equipped with an ApoTome microscope slider for optical sectioning and a cooled digital CCD camera (AxioCamMRm) using filter set 38 HE (excitation at 470 nm, beam splitter at 495 nm, and emission at 525 nm). To follow fungal development in the tissue, we constructed frequency distributions over the developmental stages sampling at least 100 individual specimens.
As to have a closer look at fungal growth within the plant tissue prior to full expression of symptoms with both GY11-EV virulent strain and GY11-AvrPia avirulent strain, samples from 1, 2 and 3 dpi were used for histological analysis. To get a quantitative approach, fungal growth was classified into the following categories: S non-germinated spore, SG germinated spore, SGA germinated spore with appressorium, IHO invasive hyphae in one cell, and IHM invasive hyphae in multiple cells (Fig. S1). In addition to the classification of the pathogen, the response of the host tissue in the neighbourhood of the spore was classified with respect to absence or presence of a hypersensitive reaction (Fig. S1). It has to be mentioned that stage S (ungerminated spores) and SG (germinated, but not attached) were not considered in quantification of fungal development, because these stages are mostly lost during the staining procedure (Figs. 1d-f and 2d-f).
Inducibility of defence-related genes by jasmonate
To investigate the inducibility of defence-related genes by jasmonate, we sprayed 100 μM (approximately 5 ml for each seedling) of Methyl Jasmonic Acid (MeJA) including 0.5% gelatine, or as mock solution, 0.5% gelatine only, to wildtype Nihonmasari seedlings at the four-leaf stage, raised as mentioned above. The fourth leaf was excised after 24 h of incubation and immediately frozen in liquid nitrogen for RNA extraction and gene expression analysis.
RNA extraction, cDNA synthesis and real-time qPCR
RNA was extracted from leaf samples of both, MeJA application assay at 24 hpi as well as the Magnaporthe infection assay at 2 and 3 dpi were used in this procedure, reversely transcribed into cDNA, and used to measure steady-state transcript levels of target genes (Supplementary Table S1) by Real-Time qPCR (CFX-96, BioRad, Munich) as described previously [48]. The genes for transcript analysis were selected on the principle that these genes were either associated with jasmonate biosynthesis and signalling, or related to defence and reported to be regulated by jasmonate. In the case of jasmonate signalling genes, selection of only five JAZ genes for the analysis was established on a pre-screening test in which these genes were more pronouncedly induced by mechanical wounding (data not shown). The expression of the genes was normalised to the expression of glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) as housekeeping gene as per the 2−ΔΔCT method [49]. The stability of house-keeping gene in expression across all the samples was validated in a preliminary test by comparison with three alternative house-keeping genes, coding for two ubiquitins (UBI5, UBI10), and one actin (ACT11) as described in [50]. To minimise the technical variation between samples and measurements in the qPCR assay, we adopted a strategy, where all samples were run on the same plate and also the reference gene was always measured in the same run with the genes of interest. For each treatment, three biological replicates were conducted, each in 2–3 technical replicates, to obtain robust results. For accession numbers, description of gene functions as well as primer sequences of the genes measured in this study see Supplementary Table S1.
Statistical analysis
We conducted the statistical analysis using the add-in Real Statistics Resource Pack (http://www.real-statistics.com/free-download/real-statistics-resource-pack/) for Excel. We used a homoscedastic, two-tailed t-test to test differences in disease severity and fungal growth between the JA-deficient mutants and the Nihonmasari wild type, where significant differences (P < 0.05) are indicated by *, and highly significant difference (P < 0.01) with **. For the multiple comparison of different transcript levels between any pair of treatments during compatible and incompatible interactions, we employed a One-Way ANOVA followed by Duncan’s test using the R package Agricolae, where significant differences (P < 0.05) are indicated by different letters above each bar. In the case of histology and transcript analysis, we conducted three biological replicates (in technical triplicates). For each biological replicate, we pooled segments from 3 to 4 individual seedlings. For the histological analysis, we investigated segments from 2 to 3 individual seedlings per experiment.
Results
During incompatible interaction, jasmonate mutants showed stronger symptoms and faster colonisation
To validate the previously reported [13] phenotype of the JA-mutants with respect to incompatible interaction, we inoculated with the avirulent strain GY11-AvrPia of M. oryzae. Here, the wild type Nihonmasari was clearly more resistant compared to cpm2 and hebiba: in both mutants, lesions covered almost twice the area as compared to the wild type (Fig. 1c). In contrast, there was no significant difference as for the lesion number per leaf area among the three genotypes (Fig. 1b), indicative of a situation, where not the initiation of a lesion, but its expansion differed between mutants and wild type.
Since the individual spores are only loosely connected with the host tissue prior to appressoria formation, they are mostly washed off during the staining procedure. It did not make sense, therefore, to include these early stages into the quantitative analysis. Thus, we could only score the stages SGA, IHO, and IHM in a reliable manner (Fig. S1). When we followed colonisation by the avirulent strain GY11-AvrPia over time, we saw a clear progression through colonisation stages. Specifically, the initial phase (1 dpi) did not reveal any difference between Nihonmasari and the two mutants (Fig. 1d): the majority of attached spores were in the state of appressorium formation (stage SGA), only few had proceeded to invade one cell (stage IHO). The frequency of these two stages were also identical to those seen for the virulent strain GY11-EV (Fig. 2d), indicating that strain differences of the host response were not manifest at this early stage. This changed drastically during the subsequent days. From 2 dpi, we observed a distinct hypersensitive reaction for the avirulent strain (Fig. 1e), manifest from appressorium formation. Infected cells without a hypersensitive reaction were almost absent – no matter, whether in the wild type or the two mutants, which remained true also for 3 dpi (Fig. 1f). However, the progression from the appressorium stage into the subsequent stages (hyphae invading individual or multiple cells) was swifter and more pronounced in amplitude in the two mutants, as compared to the wild type (Fig. 1e and f). Thus, colonisation was more successful in the mutants, resembling the situation seen for the virulent strain GY11-EV (Fig. 2e and f). However, the avirulent strain exhibited a significantly higher difference between wild type and mutants, albeit a hypersensitive reaction was also seen in the mutants.
During compatible interaction, jasmonate biosynthesis mutants showed similar symptoms, but faster colonisation
The situation was slightly different in case of compatible interaction, when we inoculated with the strain GY11-EV. Here, symptom expression in Nihonmasari wild type, and the two jasmonate deficient mutants cpm2 and hebiba did not display significant differences (Fig. 2a, b and c) as reflected also by the quantitative indicators (lesion number / leaf area and lesion area / leaf area). Specifically, the number of lesions in the cpm2 mutant was mildly (by around 25%), that in the hebiba mutant more substantially (by almost 50%) increased over the number in the wild type, but in none of the mutants was the difference significant (Fig. 2b). Also, for the coverage of lesion area, there was no significant difference (Fig. 2c). Taken together, susceptibility in cpm2 and hebiba to the virulent strain GY11-EV was comparable to their wild-type background Nihonmasari. For the cytological analysis in compatible interaction, only the stages SGA, IHO, and IHM (Fig. S1) were scored for the purpose of reliability due to the same reason as described above. When we followed colonisation by the virulent strain GY11-EV over time, we saw a clear progression through subsequent stages: while at 1 dpi most individuals just had formed appressoria (stage SGA, Fig. 2d), at 2 dpi many had invaded individual cells (stage IHO, Fig. 2e), and at 3 dpi many had proceeded to infect multiple cells (stage MHO, Fig. 2f). It should be noted that stages SGA and IHO represent steady states between increase by spores that germinated with some delay and decrease by progression to the next stage. While colonisation proceeded in both wild type and mutant hosts, there were specific differences: In the wild type, from those individuals that had formed appressoria at 1 dpi, around half had invaded the host cells at 2 dpi (roughly half of those just one cell, the other half multiple cells). The other half remained arrested at the stage of appressoria formation. This conclusion is based on the fact that, at 3 dpi, still half of the population had not advanced beyond the appressoria stage, while the sum of stages IHO and MHO had remained the same as 2 dpi. In other words, almost 60% of individuals in stage SGA had not contributed to the subsequent stages during the time interval between 2 dpi and 3 dpi. For the two jasmonate-synthesis mutants, the progression from appressoria formation to the invasion of individual cells (2 dpi) was significantly accelerated, which also holds true for the invasion into neighbouring cells (3 dpi). In contrast to the wild type, there was also a significant recovery in the frequency of cells in stage SGA at 3 dpi as compared to 2 dpi (Fig. 2e, f), indicative of a second wave of appressoria coming from hitherto non-germinated spores. Thus, invasion of virulent GY-EV strain into cells and progression into neighbouring cells proceeded more swiftly in hosts that are not able to activate jasmonate signalling.
Inducibility of defence-related genes by MeJA
To test the inducibility of defence-related genes (based on their reported inducibility by Magnaporthe infection) by jasmonate, we treated wild-type rice with 100 μM of MeJA and scored the induction of steady-state transcript levels at 24 h after inoculation (Fig. S2). The tested genes were selected from four categories, namely jasmonate biosynthesis genes (OsAOS1, OsAOS2, OsAOC, OsOPR7 and OsJAR1), jasmonate signalling genes (OsJAZ8, OsJAZ9, OsJAZ10, OsJAZ11 and OsJAZ13), defence-related genes (OsPR1a, OsPR1b, OsBBTI2, OsPBZ1, OsCPS2 and OsCPS4), and genes involved in phenylpropanoid pathway (OsPAL1, OsNOMT, OsCAD2 and OsCOMT1). The specific function of the genes is detailed in the subsequent sections. Most transcripts showed mild inductions below one order of magnitude. However, some transcripts showed a more prominent induction: OsJAZ8 was induced 25-fold, OsJAZ11, OsBBTI2, and OsNOMT around 15-fold. The genes were highly responsive to MeJA, thus being good candidate genes for following transcript analysis in jasmonate-deficient mutants. Even though some other genes are reported to be highly induced by Magnaporthe infection, their inducibility by MeJA was not as significant as expected. For instance, defence-related genes such as OsPR1a, OsPR1b, OsPBZ1, OsCPS2 and OsCPS4 are key players positively regulated by jasmonate signalling, but their transcripts were only slightly or hardly affected by MeJA treatment (Fig. S2). A similar induction pattern was also found for other jasmonate biosynthesis, jasmonate signalling genes, and for phenylpropanoid pathway genes. Nevertheless, this does not preclude that these genes might show a transient response already declined at the time point of scoring transcripts. Since the responsiveness to MeJA might not completely correlate with the responsiveness to Magnaporthe infection, all selected genes would be still used for subsequent transcription analysis for infected wild type and jasmonate mutants.
Jasmonate synthesis: OsAOS2 is a key gene during incompatible interaction
Since it was clear that the JA deficient mutants were less resistant to the M. oryzae as compared to their wildtype background ‘Nihonasari’, we were probing for potential differences in genes expression correlated to differential resistance. As both mutants were affected in the locus for the JA-biosynthesis gene allene oxide cyclase (AOC), we measured the response of JA biosynthesis genes.
The steady-state transcript levels of OsAOS1, OsAOS2, OsOPR7, OsAOC and OsJAR1 were generally lower in cpm2 and hebiba, as compared to the wild type (Fig. 3). Moreover, the response of these transcripts to fungal inoculation was almost non-existent. There was one exception, though: OsAOS2 was induced rapidly (from 2 dpi) and strongly (around 10 folds) during incompatible interaction with GY11-AvrPia in the wild type (Fig. 3b). This distinctive expression pattern of OsAOS2 was also validated by hierarchical clustering in a heatmap, where all the jasmonate biosynthesis genes except OsAOS2 were clustered in one group (Fig. S3a). In cpm2, this response was slightly reduced in amplitude (around 8 folds at 2 dpi) and less stable (at 3 dpi it had already dropped to around 2-fold (as compared to more than 8-fold in the wild type). For hebiba, the pattern was different. Here, the induction was delayed, but with an amplitude similar to the wild type. For compatible interaction with GY11-EV, we saw the response as well, but with a delay by 1 day and did not reach the same amplitude. For the wild type, an induction of around 6-fold was seen for wild type, but also for cpm2, while it was only 2-fold in hebiba. Thus, induction of OsAOS2 occurs specifically during fungal infection. This induction is stronger during incompatible interaction. In the two mutants, it occurs with reduced amplitude (cpm2) or initiates later (hebiba).
Jasmonate signalling: OsJAZ9 is the key gene during incompatible interaction
Since the jasmonate deficient mutants were impaired in their defence against the fungus, we wondered if the transcription of genes involved in jasmonate response like OsJAZ could be impaired. Hereby, we scrutinised OsJAZ8, 9, 10, 11 and 13, because they had been reported as wound-inducible [30]. In the beginning, we also considered OsJAZ12, but did not pursue it later. The reason was a non-steady melting curve of the qPCR amplification, indicating inhomogeneities. Therefore, the readout was not reliable. Unfortunately, the nomenclature of rice JAZ genes is not standardised. For the sake of clarity, we have therefore decided to follow the nomenclature given in [20]. Generally speaking, the expression pattern of JAZ genes was clearly clustered in two groups in hierarchical clustering analysis in which JAZ9 was separated from other JAZ genes (Fig. S3b). Specifically, except for JAZ9, the transcript responses of other JAZ genes were of minor amplitudes (in the range of 1 to 5-fold), but still it became evident that both mutants were accumulating significantly lower steady-state levels of OsJAZ8 (Fig. 4a), OsJAZ10 (Fig. 4c), OsJAZ11 (Fig. 4d), and OsJAZ13 (Fig. 4e) as compared to the wild type. These relatively sluggish responses contrasted sharply with the pattern observed for OsJAZ9 (Fig. 4b). Here, the wild type reached much higher levels (more than 15-fold as compared to the control). Like the pattern observed for OsAOS2, the accumulation of OsJAZ9 transcripts became manifest earlier (already at 2 dpi) for the incompatible interaction with GY11-AvrPia, while for the compatible interaction with GY11-EV, this accumulation was reached only 1 day later. The mutants did respond as well, however, the amplitudes where not comparable (remaining below 5-fold induction, Fig. 4b), which is in stark contrast to the situation seen for OsAOS2, where the mutants did reach up to comparable transcript levels as the wild type (Fig. 3b). However, ignoring these differences in relative amplitude, the temporal patterns of OsAOS2 and OsJAZ9 transcription were comparable as validated in Fig. S3c. In cpm2, the response of OsJAZ9 was seen already at 2 dpi (albeit with low amplitude) and found to decline, if scored 1 day later, while for hebiba, the response was still low at 2 dpi, but did increase rather than decline during the following day. The same temporal pattern had been seen in case of OsAOS2, however, the responses of JAZ9 in the mutants where much lower, although significant. This leads to the conclusion that OsJAZ9 is induced specifically during fungal infection. This induction is faster during incompatible interaction, and it was strongly impaired in the jasmonate biosynthesis mutants.
Jasmonates negatively regulate the induction of OsPR1b and OsCPS2
In the next step, we probed the expression of a panel of defence-related genes reported to be jasmonate responsive, to get insight into specific downstream pathways: The pathogenesis-related protein OsPR1a belongs to the acidic PR proteins, reported to be upregulated by MeJA [51]. In parallel, OsPR1b encoding the most prominent member of basic PR proteins, was probed, for which the literature record with respect to JA responsiveness was discrepant (no induction by 100 μM MeJA reported by [51], strong induction by 100 μM JA by [12]. In a third report, the inducibility of OsPR1a and OsPR1b by JA or SA or Magnaporthe had been d found only in the old, but not the young leaves of rice [52].Also, the transcript encoding the Bowman-Birk protease inhibitor BBTI2 [23], and the OsPR10a / PBZ1 coding for a RNase, both reported as JA inducible [53], were included into the study. In addition, we addressed two transcripts for ent-copalyl diphosphate synthases, OsCPS2 and OsCPS4, because they are involved in the formation of labdane-related diterpenoids, discussed as phytoalexins [54]. In the same work, a knockdown of OsCPS2, but not of OsCPS4 caused a modulated response to M. oryzae. Both genes respond to the jasmonate pathway [55].
The resulting patterns (Fig. 5) were clearly specific for the type of gene, the type of interaction (compatible or incompatible), and for the host genotype. Overall, OsPR1b and OsCPS2 sticked out specifically among the tested jasmonate-responsive transcripts responsive to M. oryzae. This was based on two observations: (1) both transcripts showed a more substantial accumulation in mutants, indicative of a negative regulation by jasmonates; (2) both transcripts showed an earlier, but transient amplification in cpm2, while the induction in hebiba initiates later, but is more persistent thereafter, indicating that in addition to the impaired allene oxide cyclase activity other genetic factors absent in hebiba (but still present in cpm2) played a role in the regulation of OsPR1b and OsCPS2 genes (Fig. 5b and e). The distinct role of OsPR1b and OsCPS2 was also verified in the hierarchical clustering analysis, with the cluster of OsCPS2 and OsPR1b being clearly separated from other genes (Fig. S3d). Specifically, OsPR1b encoding a basic PR protein, known as strongly inducible during Magnaporthe infection, was rapidly and transiently induced during incompatible interaction, with a delay as well as a lower amplitude during compatible interaction (Fig. 5b), while OsCPS2 encoding an ent-copalyl diphosphate synthase displayed a similar and strong induction pattern (Fig. 5e). Thus, the transcript of OsPR1b and OsCPS2 shared the following pattern details – earlier induction during incompatible interaction, amplified response in the mutants as compared to the wild type, swifter, and transient response in cpm2, delayed but stronger response in hebiba (Fig. 5b and e). In contrast, transcription patterns of OsPR1a (encoding an acidic PR protein) and OsBBTI2 (a Bowman-Birk protease inhibitor) clustered more closely, both not being differently induced in the compatible interaction between WT and mutants until later stage of infection (namely 3 dpi), but earlier and significantly diverging during the incompatible interaction between WT and mutants (Fig. 5a and c). The induction of OsPR1a and OsBBTI2 seemed to require jasmonate biosynthesis, since it was absent in both mutants. The response of PBZ1 (Fig. 5d) was of a comparatively minor amplitude, again initiating earlier during incompatible interaction. Here, the mutants were not significantly different from the wild type, with one surprising exception: during day 3 of the incompatible interaction, there was a strong upregulation in case of hebiba as a host, while cpm2 only produced the same induction seen in the wild type. Similarly, the induction of OsCPS4 (coding for another ent-copalyl diphosphate synthase) was of a relatively low amplitude in both compatible and incompatible interactions, and was found significantly higher in mutants (either cpm2 or hebiba) than wild type (Fig. 5f).
Jasmonates are required for the activation of a phytoalexin synthesis gene
The accumulation of antifungal defence compounds (phytoalexins) represents an efficient response against invasion. Many plants accumulate phenolic compounds by activation of the phenylpropanoid pathway. In rice, the flavonoid sakuranetin [56] is one of the most relevant phytoalexins. We therefore probed several genes of the phenylpropanoid pathway (Fig. 6e), such as phenyl ammonium lyase (PAL) as first committed step of secondary metabolism, along with naringenin 7-O-methyltransferase (NOMT), the enzyme that gives rise to sakuranetin. To probe for the activity of the monolignol branch of the phenylpropanoid pathway, the transcripts for cinnamyl alcohol dehydrogenase 2 (CAD2), the first committed step of monolignol biosynthesis in rice, along with those for caffeic acid O-methyltransferase (COMT) catalysing the subsequent step giving rise to ferulic acid were measured (Fig. 6e). In the wild type, transcripts for PAL (Fig. 6a) showed only a minute, but significant induction by around twofold. This induction occurred more swiftly in case of incompatible interaction. This induction was also present in cpm2, but delayed in hebiba. Instead, there was a clear and strong response for the NOMT transcripts (Fig. 6b), exceeding one order of magnitude at day 3 and developing more swiftly during incompatible as compared to compatible interaction. This response was completely absent from the mutants indicating that the response of NOMT to infection is strictly dependent on jasmonates. The two tested transcripts representing the monolignol branch were induced (Fig. 6c and d), albeit to a lesser extent (around 5-fold), and with a less strict dependence on jasmonates, because, here, the two mutants still were able to produce a partial response. Thus, NOMT as phytoalexins-synthesis gene clearly sticks out among the four tested candidates of the phenylpropanoid pathway as the transcript with the strongest response. The point was also confirmed in the hierarchical clustering, with NOMT as an independent cluster among the four phenylpropanoid metabolism related genes (Fig. S3e). It was also the transcript with the strictest dependence on jasmonates.
Discussion
Our motivation for the current work was to compare the role of the jasmonate pathway for the resistance against and the response to virulent or avirulent strains of M. oryzae. The strains were isogenic and differed only with respect to the cognate avirulence/resistance genes avrPia/Pia. This allowed to address, more specifically and independently of the pathogen background, incompatible and compatible interactions.
We observe that the jasmonate-deficient mutants, cpm2 and hebiba, develop stronger symptoms and allow a faster progression of fungal development. This enhanced susceptibility in mutants correlates with the specific lack of induction of transcripts for OsAOS2 (JA biosynthesis), OsJAZ9 (JA signalling), and OsNOMT (JA-dependent phytoalexin synthesis), especially for incompatible interaction. The fact that JA can suppress some of the defence-related genes, such as OsPR1b, OsCPS2, and that infection and exogenous methyl jasmonate induce different patterns of transcript accumulation indicate that fungal interaction triggers additional pathways in parallel to jasmonate synthesis and signalling. In the following, we will discuss to what extent the jasmonate pathway can orchestrate defence against M. oryzae, whether compatible and incompatible interaction differ in this respect, and what other signals might be involved.
Jasmonate signalling is necessary for defence against M. oryzae
Both jasmonate-depletion mutants (cpm2 and hebiba) showed a more pronounced expression of symptoms (Fig. 1a, b and c) and a faster colonisation of host cells (Fig. 1d, e and f) upon infection in the incompatible interaction. This was consistent with previous observations conducted on detached leaf sheath [13] and extends the phenomenon to entire living plants. Moreover, the faster or more extended invasion (Fig. 1d, e and f) can also explain the larger visible necrosis (Fig. 1a) observed in both mutants upon initiation of hypersensitive response. This is also congruent with the finding that osjar1 mutant (Tos17 insertion mutant affected in the conjugation of isoleucine and, thus, deficient in JA-Ile) was more susceptible than its parental wild type, as well as displaying significantly bigger necrotic lesions during incompatible interaction [30]. Even though the visual difference was not reaching significance in the case of compatible interaction (Fig. 2a, b and c), when we scrutinised the stages of infection (Fig. 2d, e and f), we saw that the transition from formation of an appressorium towards invasion of the first cell represented a bottleneck in case of the wild type, while this step proceeded more swiftly in the mutants. This is in line with the report that the jasmonate over-accumulation restrains fungal growth during compatible interaction compared to the wild type situation [12]. This cytological difference between wild type and jasmonate mutants was independent of the type of interaction, because we saw a similarpattern for both, compatible (Fig. 2e and f) and incompatible interaction (Fig. 1e and f), where, especially at 2 dpi, a hypersensitive response of the host cell ensued (Fig. 1). Only at 3 dpi, differences between interaction types emerged, when more than 20% of infection events were arrested in the first cell (IHO stage) for incompatible interaction, in contrast to less than 5% in compatible interaction. However, still fungal colonisation remained more efficient in both mutants compared to the wild type (Figs. 2f and 3f). Altogether, the jasmonate-deficient mutants (cpm2 and hebiba) were impaired in defence against M. oryzae in both, incompatible and compatible, interactions.
For the wild type, the delay in the transition from appressorium formation to invasion into the first host cell came with a specific induction of transcripts for OsJAZ9 (Fig. 4b), OsAOS2 (Fig. 3b) and OsNOMT, the key enzyme for the phytoalexin sakuranetin (Fig. 6b). The response of OsNOMT was completely absent in the mutants, irrespectively of the type of interaction, indicative of an absolute requirement for jasmonate synthesis. The induction of OsJAZ9 was mostly, but not entirely absent in the mutants, reporting a strong but not exclusive dependence on jasmonates. In contrast, the induction of OsAOS2 was not eliminated, but just delayed, suggesting that a jasmonate-independent pathway can convey the signal, but requires jasmonates to proceed swiftly. The fact that OsJAZ9 and OsAOS2 are partially under control of a jasmonate-independent signal is also consistent with the map of MeJA responsiveness (Fig. S2), where both transcripts were not prominent with respect to induction. This is contrasting with OsNOMT, which exhibited a conspicuous response to MeJA.
These findings lead to a model (Fig. 7), where infection with M. oryzae activates jasmonate synthesis and sakuranetin accumulation (probably through OsJAZ9), which helps to impair the step from appressorium formation to hyphal penetration. In parallel, a jasmonate-independent event activates OsAOS2 in concert with positive feedback from jasmonate signalling. This mechanism is active for both fungal strains but proceeds more swiftly when the interaction is incompatible.
The published record has already demonstrated important implications of this model. For instance, the UV-response of OsNOMT is strictly dependent on bioactive jasmonates. The osjar1 mutant is not able to conjugate isoleucine to jasmonate, and likewise fails to induce OsNOMT in response to UV [57]. Likewise, the key role of OsAOS2 is in line with a study, where overexpression of OsAOS2 enhanced the induction of the defence-genes PR1a, PR3 and PR5 upon infection in compatible interaction [12]. A candidate for the feedback of jasmonate signalling on OsAOS2 expression might be specific WRKY factors. In Arabidopsis, herbivory through Spodoptera exigua results in a calcium dependent phosphorylation of the JAV1-JAZ8-WRKY51 protein complex that can de-repress AOS genes [58]. Specificity on the level of JAZ-proteins is also in line with the finding that resistance of rice to the bacterial pathogen Xanthomonas oryzae pv. oryzae is regulated by a different member (OsJAZ8) of this family [41].
OsAOS2 and OsJAZ9 are two key players in JA-dependent pathway
While the majority of jasmonate-synthesis related transcripts were not very responsive to infection, OsAOS2 was clearly behaving differently as also validated by hierarchical clustering (Fig. S3a). During incompatible interaction, this transcript increased strongly and swiftly (Fig. 3c), while this response occurred 1 day later during compatible interaction. The two mutants were able to deploy this response, albeit at somewhat reduced amplitude (cpm2) or at a later time point (hebiba), suggesting a pivotal role of OsAOS2 during incompatible interaction. The fact that overexpression of OsAOS2 in rice (causing elevated jasmonate levels) led to elevated resistance against a moderately virulent strain of M. oryzae [12] would support the notion that OsAOS2 is also one of the factors underlying basal immunity in compatible interaction. A straightforward hypothesis would be that the ability to potently induce jasmonate biosynthesis is hall marker for strong basal immunity in both compatible and incompatible interactions. If this holds true, one would expect that jasmonate synthesis or stability are target for pathogen effectors. This is consistent with the fact that the virulent strain of M. oryzae GY11 uses the microRNA miR319 to quell the JA biosynthesis genes OsLOX2 and OsLOX5 [59]. Likewise, virulent strains of M. oryzae can secrete a monooxygenase that converts JA in rice plant to 12OH-JA (inactive in JA defence signalling), thereby weakening basal immunity and facilitating colonisation in host [60]. Moreover, exogenous JA increased the resistance to a virulent strain of M. oryzae [13]. In addition, the induction of OsAOS2 seems to proceed via a JA-independent pathway (as we can conclude that it was also present in the mutants), albeit JA seems to promote this pathway (as we can conclude from the lower or later response in the mutants).
In contrast, OsJAZ9, acting as crucial jasmonate response factor during defence to M. oryzae (Fig. S3b), was strictly depending on JA, since this response was completely absent in the mutants (Fig. 4b). Its transcription pattern in response to M. oryzae infection was clustered together with OsAOS2 among the jasmonate biosynthesis and signalling genes, pointing to that OsJAZ9 is also regulated in the same pathway as that activating OsAOS2. Similarly, its response was 1 day earlier during incompatible as compared to compatible interaction, consistent with the hypothesis that fungal effectors (such as miR319) [59] silence JA biosynthesis genes or activate JA catabolism [60] to delay host basal immunity. Taken together, OsJAZ9 is another critical player in combination with OsAOS2 in potentiation of basal immunity.
SA might be involved in the OsJAZ9-independent pathway
Among potential downstream genes, some show different patterns, where the induction in jasmonate-deficient mutants is stronger than in the wild type, indicative of involvement of an OsJAZ9-independent pathway. In this context, the strong and specific response of the acidic PR protein OsPR1b (Fig. 5b) is worth mentioning because it increased more strongly JA mutants, in both compatible and incompatible interactions. Although this gene is responsive to exogenous jasmonates [12, 61], it remains responsive in the mutants as well, and is even inducible to a higher degree. Thus, this response does not require jasmonates and seems to run independently of OsJAZ9. Since OsPR1b can also be strongly induced by salicylic acid, suggesting that this second pathway involving salicylic acid can sometimes act in synergy with jasmonate signalling in terms of M. oryzae resistance [21, 51]. A similar conclusion was reached for OsCPS2. This was also confirmed in hierarchical clustering analysis in which OsPR1b and OsCPS2 clustered differently from OsJAZ9 (Fig. S3f), suggesting that the OsJAZ9-independent pathway was involved in regulating OsPR1b and OsCPS2. Similar conclusions derive from the patterns for OsPBZ1 (Fig. 5d) and OsCPS4 (Fig. 5f) that, in certain cases, are more responsive in the mutants as well. Both genes have been reported to be independent of JA [62, 63], again corroborating a role for OsJAZ9-independent signalling. A candidate for such a SA-dependent pathway would be signalling through OsWRKY45. In fact, the SA analog benzothiadiazole was shown to prime rice plants to rapidly upregulate OsCPS2 and OsCPS4 transcripts and subsequent diterpene phytoalexin production in response to Magnaporthe infection in a OsWRKY45-dependent manner [63].
OsNOMT is a marker gene of the JA-dependent pathway
In addition to the transcript with the most pronounced JA-dependence, OsNOMT (Fig. 6b), also the transcript of the Bowman-Birk trypsin inhibitor OsBBTI2 responded more swiftly during incompatible interaction in a strict dependence on jasmonates, as evident from the lacking induction in the mutants (Fig. 5c). Further support comes from the strong response of this transcript to exogenous MeJA (Fig. S2), which was more conspicuous than the induction upon infection. This inhibitor, in concert with heat shock transcription factor HSF23, mediates constitutive defence against M. oryzae in rice [64]. Even though the transcription of OsPR1a displayed a certain JA-dependence as well, this was less strict since the transcript level during incompatible interaction at 3 dpi were comparable between hebiba and wildtype (Fig. 5a). Since hebiba harbours multiple gene deletions extending beyond the lack of OsAOC, the difference to cpm2 indicates that JA is not the main player here. This is also congruent with the publishedrecords, where OsPR1a has been reported to be strongly inducible in response to SA and H2O2, and moderately by abscisic acid [65]. The same holds true for OsCAD2 (Fig. 6c) and OsCOMT1 (Fig. 6d). Both transcripts were positively correlated with JA levels in most cases (less induced but not abolished in JA mutants), indicating a role in JA-dependent signalling in this specific scenario. Both genes encode enzymes for lignin biosynthesis in rice, which might be relevant as physical barrier to pathogen penetration [66,67,68]. Altogether, it is OsNOMT that qualifies as best marker gene for JA signalling, followed to some extent by OsBBTI2.
Outlook - is SA the second player during incompatible interaction?
Both mutants display necrotic lesions that extend beyond the degree seen in the wildtype (Fig. 1a). This allows two conclusions. First, PCD seems to proceed independently of jasmonate signalling. Second, JA might be a negative regulator of PCD itself, or, more likely, impair cell-cell spread of the pathogen.
The literature record on the role of JA in PCD is quite discrepant, to put it mildly. In some cases, it seems to attenuate PCD by antagonistic crosstalk with SA-mediated PCD [35, 38] or constraining concurrent 9-LOX derived oxylipin signalling [69,70,71]. In other cases, JA enhances stress induced PCD by synergy with SA signalling [36, 37]. This discrepancy may stem from the fact that the functional context matters and that possible different types of PCD are involved [72].
To get more insight in the role of salicylic acid, we are currently analysing its interaction with jasmonate dependent signalling, because this interaction might be a target of co-evolution between M. oryzae and its host, O. sativa.
Both JA mutants displayed significant difference from the wild type with respect to the cytological aspects of both, compatible and incompatible interactions, especially at 2 dpi. Likewise, as discussed above, the activation of OsAOS2 at 2 dpi most likely involves factors that are independent of JA. Even though WRKY transcription factors qualify as potential candidates, we still need to address this experimentally, for instance, by conducting a transcriptomic analysis during the early stages of response (1 dpi or even earlier) in order to identify specific factors responsible for the observed increase of susceptibility in jasmonate-deficient mutants and for the induction of OsAOS2.
Availability of data and materials
The data used and analyzed in this study are available from the corresponding author on reasonable request.
References
Nalley L, Tsiboe F, Durand-Morat A, Shew A, Thoma G. Economic and environmental impact of rice blast pathogen (Magnaporthe oryzae) alleviation in the United States. PLoS One. 2016;11(12):e0167295. https://doi.org/10.1371/journal.pone.0167295.
Serrano M, Coluccia F, Torres M, L'Haridon F, Metraux JP. The cuticle and plant defense to pathogens. Front Plant Sci. 2014;5:274–82. https://doi.org/10.3389/fpls.2014.00274.
Osbourn AE. Preformed antimicrobial compounds and plant defense against fungal attack. Plant Cell. 1996;8(10):1821–31. https://doi.org/10.1105/tpc.8.10.1821.
Piasecka A, Jedrzejczak-Rey N, Bednarek P. Secondary metabolites in plant innate immunity: conserved function of divergent chemicals. New Phytol. 2015;206(3):948–64. https://doi.org/10.1111/nph.13325.
Vergne E, Grand X, Ballini E, Chalvon V, Saindrenan P, Tharreau D, et al. Preformed expression of defense is a hallmark of partial resistance to rice blast fungal pathogen Magnaporthe oryzae. BMC Plant Biol. 2010;10(1):206–23. https://doi.org/10.1186/1471-2229-10-206.
Li P, Lu YJ, Chen H, Day B. The lifecycle of the plant immune system. Crit rev. Plant Sci. 2020;39(1):72–100. https://doi.org/10.1080/07352689.2020.1757829.
Cook DE, Mesarich CH. Thomma BP: understanding plant immunity as a surveillance system to detect invasion. Annu Rev Phytopathol. 2015;53:541–63. https://doi.org/10.1146/annurev-phyto-080614-120114.
Peng Y, van Wersch R, Zhang Y. Convergent and divergent signaling in pamp-triggered immunity and effector-triggered immunity. Mol Plant Microbe Interact. 2018;31(4):403–9. https://doi.org/10.1094/MPMI-06-17-0145-CR.
Jones JD, Dangl JL. The plant immune system. Nature. 2006;444(7117):323–9. https://doi.org/10.1038/nature05286.
Robert-Seilaniantz A, Grant M, Jones JD. Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism. Annu Rev Phytopathol. 2011;49:317–43. https://doi.org/10.1146/annurev-phyto-073009-114447.
Feussner I, Wasternack C. The lipoxygenase pathway. Annu Rev Plant Biol. 2002;53(1):275–97. https://doi.org/10.1146/annurev.arplant.53.100301.135248.
Mei C, Qi M, Sheng G, Yang Y. Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection. Mol Plant Microbe Interact. 2006;19(10):1127–37. https://doi.org/10.1094/mpmi-19-1127.
Riemann M, Haga K, Shimizu T, Okada K, Ando S, Mochizuki S, et al. Identification of rice allene oxide cyclase mutants and the function of jasmonate for defence against Magnaporthe oryzae. Plant J. 2013;74(2):226–38. https://doi.org/10.1111/tpj.12115.
Guan L, Denkert N, Eisa A, Lehmann M, Sjuts I, Weiberg A, et al. JASSY, a chloroplast outer membrane protein required for jasmonate biosynthesis. Proc Natl Acad Sci U S A. 2019;116(21):10568–75. https://doi.org/10.1073/pnas.1900482116.
Tani T, Sobajima H, Okada K, Chujo T, Arimura S-i, Tsutsumi N, et al. Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice. Planta. 2008;227(3):517–26. https://doi.org/10.1007/s00425-007-0635-7.
Wakuta S, Suzuki E, Saburi W, Matsuura H, Nabeta K, Imai R, et al. OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signalling. Biochem Biophys Res Commun. 2011;409(4):634–9. https://doi.org/10.1016/j.bbrc.2011.05.055.
Svyatyna K, Jikumaru Y, Brendel R, Reichelt M, MithÖfer A, Takano M, et al. Light induces jasmonate-isoleucine conjugation via OsJAR1-dependent and -independent pathways in rice. Plant Cell Environ. 2014;37(4):827–39. https://doi.org/10.1111/pce.12201.
Ogawa S, Miyamoto K, Nemoto K, Sawasaki T, Yamane H, Nojiri H, et al. OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability. Sci Rep. 2017;7:40175–89. https://doi.org/10.1038/srep40175.
Uji Y, Taniguchi S, Tamaoki D, Shishido H, Akimitsu K, Gomi K. Overexpression of OsMYC2 results in the up-regulation of early JA-rresponsive genes and bacterial blight resistance in rice. Plant Cell Physiol. 2016;57(9):1814–27. https://doi.org/10.1093/pcp/pcw101.
Ye H, Du H, Tang N, Li X, Xiong L. Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice. Plant Mol Biol. 2009;71(3):291–305. https://doi.org/10.1007/s11103-009-9524-8.
Thaler JS, Humphrey PT, Whiteman NK. Evolution of jasmonate and salicylate signal crosstalk. Trends Plant Sci. 2012;17(5):260–70. https://doi.org/10.1016/j.tplants.2012.02.010.
Tamaoki D, Seo S, Yamada S, Kano A, Miyamoto A, Shishido H, et al. Jasmonic acid and salicylic acid activate a common defense system in rice. Plant Signal Behav. 2013;8(6):e24260. https://doi.org/10.4161/psb.24260.
Rakwal R, Kumar Agrawal G, Jwa NS. Characterization of a rice (Oryza sativa L.) Bowman-Birk proteinase inhibitor: tightly light regulated induction in response to cut, jasmonic acid, ethylene and protein phosphatase 2A inhibitors. Gene. 2001;263(1–2):189–98. https://doi.org/10.1016/s0378-1119(00)00573-4.
De Vleesschauwer D, Xu J, Hofte M. Making sense of hormone-mediated defense networking: from rice to Arabidopsis. Front Plant Sci. 2014;5:611. https://doi.org/10.3389/fpls.2014.00611.
De Vleesschauwer D, Gheysen G, Hofte M. Hormone defense networking in rice: tales from a different world. Trends Plant Sci. 2013;18(10):555–65. https://doi.org/10.1016/j.tplants.2013.07.002.
Trang Nguyen H, Thi Mai To H, Lebrun M, Bellafiore S, Champion A. Jasmonates-the master regulator of rice development, adaptation and defense. Plants. 2019;8(9):339–58. https://doi.org/10.3390/plants8090339.
Li Y, Nie Y, Zhang Z, Ye Z, Zou X, Zhang L, et al. Comparative proteomic analysis of methyl jasmonate-induced defense responses in different rice cultivars. Proteomics. 2014;14(9):1088–101. https://doi.org/10.1002/pmic.201300104.
Nojiri H, Sugimori M, Yamane H, Nishimura Y, Yamada A, Shibuya N, et al. Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cells. Plant Physiol. 1996;110(2):387–92. https://doi.org/10.1104/pp.110.2.387.
Rakwal R, Tamogami S, Kodama O. Role of jasmonic acid as a signaling molecule in copper chloride-elicited rice phytoalexin production. Biosci Biotechnol Biochem. 2014;60(6):1046–8. https://doi.org/10.1271/bbb.60.1046.
Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, et al. OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice. Biosci Biotechnol Biochem. 2013;77(7):1556–64. https://doi.org/10.1271/bbb.130272.
Yara A, Yaeno T, Hasegawa M, Seto H, Montillet JL, Kusumi K, et al. Disease resistance against Magnaporthe grisea is enhanced in transgenic rice with suppression of omega-3 fatty acid desaturases. Plant Cell Physiol. 2007;48(9):1263–74. https://doi.org/10.1093/pcp/pcm107.
Yara A, Yaeno T, Hasegawa M, Seto H, Seo S, Kusumi K, et al. Resistance to Magnaporthe grisea in transgenic rice with suppressed expression of genes encoding allene oxide cyclase and phytodienoic acid reductase. Biochem Biophys Res Commun. 2008;376(3):460–5. https://doi.org/10.1016/j.bbrc.2008.08.157.
Liu Y, Pagac M, Yang F, Patkar RN, Naqvi NI. Fungal jasmonate as a novel morphogenetic signal for pathogenesis. J Fungi. 2021;7(9):693–712. https://doi.org/10.3390/jof7090693.
Liu L, Sonbol FM, Huot B, Gu Y, Withers J, Mwimba M, et al. Salicylic acid receptors activate jasmonic acid signalling through a non-canonical pathway to promote effector-triggered immunity. Nat Commun. 2016;7:13099–109. https://doi.org/10.1038/ncomms13099.
Rao MV, Lee H, Creelman RA, Mullet JE, Davis KR. Jasmonic acid signaling modulates ozone-induced hypersensitive cell death. Plant Cell. 2000;12(9):1633–46. https://doi.org/10.1105/tpc.12.9.1633.
Zhang L, Jia C, Liu L, Zhang Z, Li C, Wang Q. The involvement of jasmonates and ethylene in Alternaria alternata f. sp. lycopersici toxin-induced tomato cell death. J Exp Bot. 2011;62(15):5405–18. https://doi.org/10.1093/jxb/err217.
Reinbothe C, Springer A, Samol I, Reinbothe S. Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence. FEBS J. 2009;276(17):4666–81. https://doi.org/10.1111/j.1742-4658.2009.07193.x.
Andi S, Taguchi F, Toyoda K, Shiraishi T, Ichinose Y. Effect of methyl jasmonate on harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of PAL mRNA in tobacco suspension cultured BY-2 cells. Plant Cell Physiol. 2001;42(4):446–9. https://doi.org/10.1093/pcp/pce056.
Gong P, Riemann M, Dong D, Stoeffler N, Gross B, Markel A, et al. Two grapevine metacaspase genes mediate ETI-like cell death in grapevine defence against infection of Plasmopara viticola. Protoplasma. 2019;256(4):951–69. https://doi.org/10.1007/s00709-019-01353-7.
Onohata T, Gomi K. Overexpression of jasmonate-responsive OsbHLH034 in rice results in the induction of bacterial blight resistance via an increase in lignin biosynthesis. Plant Cell Rep. 2020;39(9):1175–84. https://doi.org/10.1007/s00299-020-02555-7.
Yamada S, Kano A, Tamaoki D, Miyamoto A, Shishido H, Miyoshi S, et al. Involvement of OsJAZ8 in jasmonate-induced resistance to bacterial blight in rice. Plant Cell Physiol. 2012;53(12):2060–72. https://doi.org/10.1093/pcp/pcs145.
Cai Q, Yuan Z, Chen M, Yin C, Luo Z, Zhao X, et al. Jasmonic acid regulates spikelet development in rice. Nat Commun. 2014;5:3476–89. https://doi.org/10.1038/ncomms4476.
Fu J, Wu H, Ma S, Xiang D, Liu R, Xiong L. OsJAZ1 attenuates drought resistance by regulating ja and aba signaling in rice. Front. Plant Sci. 2017;8:2108–21. https://doi.org/10.3389/fpls.2017.02108.
Liang B, Wang H, Yang C, Wang L, Qi L, Guo Z, et al. Salicylic acid is required for broad-spectrum disease resistance in rice. Int J Mol Sci. 2022;23(3):1354–93. https://doi.org/10.3390/ijms23031354.
Ke Y, Kang Y, Wu M, Liu H, Hui S, Zhang Q, et al. Jasmonic acid-involved OsEDS1 signaling in rice-bacteria interactions. Rice. 2019;12(1):25–37. https://doi.org/10.1186/s12284-019-0283-0.
Liao J, Huang H, Meusnier I, Adreit H, Ducasse A, Bonnot F, et al. Pathogen effectors and plant immunity determine specialization of the blast fungus to rice subspecies. Elife. 2016;5. https://doi.org/10.7554/eLife.19377.
Berruyer R, Adreit H, Milazzo J, Gaillard S, Berger A, Dioh W, et al. Tharreau D: identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1. Theor Appl Genet. 2003;107(6):1139–47. https://doi.org/10.1007/s00122-003-1349-2.
Tang G, Ma J, Hause B, Nick P, Riemann M. Jasmonate is required for the response to osmotic stress in rice. Environ Exp Bot. 2020;175. https://doi.org/10.1016/j.envexpbot.2020.104047.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001;25(4):402–8. https://doi.org/10.1006/meth.2001.1262.
Silver N, Best S, Jiang J, Thein SL. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 2006;7:33–42. https://doi.org/10.1186/1471-2199-7-33.
Mitsuhara I, Iwai T, Seo S, Yanagawa Y, Kawahigasi H, Hirose S, et al. Characteristic expression of twelve rice PR1 family genes in response to pathogen infection, wounding, and defense-related signal compounds. Mol Genet Genomics. 2008;279(4):415–27. https://doi.org/10.1007/s00438-008-0322-9.
Xie XZ, Xue YJ, Zhou JJ, Zhang B, Chang H, Takano M. Phytochromes regulate SA and JA signaling pathways in rice and are required for developmentally controlled resistance to Magnaporthe grisea. Mol Plant. 2011;4(4):688–96. https://doi.org/10.1093/mp/ssr005.
Rakwal R, Agrawal GK, Yonekura M. Light-dependent induction of OsPR10 in rice (Oryza sativa L.) seedlings by the global stress signaling molecule jasmonic acid and protein phosphatase 2A inhibitors. Plant Sci. 2001;161(3):469–79. https://doi.org/10.1016/S0168-9452(01)00433-2.
Lu X, Zhang J, Brown B, Li R, Rodriguez-Romero J, Berasategui A, et al. Inferring roles in defense from metabolic allocation of rice diterpenoids. Plant Cell. 2018;30(5):1119–31. https://doi.org/10.1105/tpc.18.00205.
Okada K, Abe H, Arimura G. Jasmonates induce both defense responses and communication in monocotyledonous and dicotyledonous plants. Plant Cell Physiol. 2015;56(1):16–27. https://doi.org/10.1093/pcp/pcu158.
Kodama O, Miyakawa J, Akatsuka T, Kiyosawa S. Sakuranetin, a flavanone phytoalexin from ultraviolet-irradiated rice leaves. Phytochemistry. 1992;31(11):3807–9. https://doi.org/10.1016/S0031-9422(00)97532-0.
Miyamoto K, Enda I, Okada T, Sato Y, Watanabe K, Sakazawa T, et al. Jasmonoyl-l-isoleucine is required for the production of a flavonoid phytoalexin but not diterpenoid phytoalexins in ultraviolet-irradiated rice leaves. Biosci Biotechnol Biochem. 2016;80(10):1934–8. https://doi.org/10.1080/09168451.2016.1189319.
Yan C, Fan M, Yang M, Zhao J, Zhang W, Su Y, et al. Injury activates Ca2+/calmodulin-dependent phosphorylation of JAV1-JAZ8-WRKY51 complex for jasmonate biosynthesis. Mol Cell. 2018;70(1):136–49. https://doi.org/10.1016/j.molcel.2018.03.013.
Zhang X, Bao Y, Shan D, Wang Z, Song X, Wang Z, et al. Magnaporthe oryzae induces the expression of a microrna to suppress the immune response in rice. Plant Physiol. 2018;177(1):352–68. https://doi.org/10.1104/pp.17.01665.
Patkar RN, Benke PI, Qu Z, Chen YY, Yang F, Swarup S, et al. A fungal monooxygenase-derived jasmonate attenuates host innate immunity. Nat Chem Biol. 2015;11(9):733–40. https://doi.org/10.1038/nchembio.1885.
Nahar K, Kyndt T, De Vleesschauwer D, Hofte M, Gheysen G. The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice. Plant Physiol. 2011;157(1):305–16. https://doi.org/10.1104/pp.111.177576.
Kim SG, Kim ST, Wang Y, Yu S, Choi IS, Kim YC, et al. The RNase activity of rice probenazole-induced protein1 (PBZ1) plays a key role in cell death in plants. Mol Cells. 2011;31(1):25–31. https://doi.org/10.1007/s10059-011-0004-z.
Akagi A, Fukushima S, Okada K, Jiang CJ, Yoshida R, Nakayama A, et al. WRKY45-dependent priming of diterpenoid phytoalexin biosynthesis in rice and the role of cytokinin in triggering the reaction. Plant Mol Biol. 2014;86(1–2):171–83. https://doi.org/10.1007/s11103-014-0221-x.
Grand X, Espinoza R, Michel C, Cros S, Chalvon V, Jacobs J, et al. Identification of positive and negative regulators of disease resistance to rice blast fungus using constitutive gene expression patterns. Plant Biotechnol J. 2012;10(7):840–50. https://doi.org/10.1111/j.1467-7652.2012.00703.x.
Agrawal GK, Rakwal R, Jwa N-S, Agrawal VP. Signalling molecules and blast pathogen attack activates rice OsPR1a and OsPR1b genes: a model illustrating components participating during defence/stress response. Plant Physiol Biochem. 2001;39(12):1095–103. https://doi.org/10.1016/S0981-9428(01)01333-X.
Koshiba T, Hirose N, Mukai M, Yamamura M, Hattori T, Suzuki S, et al. Characterization of 5-hydroxyconiferaldehyde O-methyltransferase in Oryza sativa. Plant Biotechnol. 2013;30(2):157–67. https://doi.org/10.5511/plantbiotechnology.13.0219a.
Ookawa T, Inoue K, Matsuoka M, Ebitani T, Takarada T, Yamamoto T, et al. Increased lodging resistance in long-culm, low-lignin gh2 rice for improved feed and bioenergy production. Sci Rep. 2014;4:6567–76. https://doi.org/10.1038/srep06567.
Miedes E, Vanholme R, Boerjan W, Molina A. The role of the secondary cell wall in plant resistance to pathogens. Front. Plant Sci. 2014;5:358–71. https://doi.org/10.3389/fpls.2014.00358.
Montillet JL, Chamnongpol S, Rusterucci C, Dat J, van de Cotte B, Agnel JP, et al. Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves. Plant Physiol. 2005;138(3):1516–26. https://doi.org/10.1104/pp.105.059907.
Marmey P, Jalloul A, Alhamdia M, Assigbetse K, Cacas JL, Voloudakis AE, et al. The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum. Plant Physiol Biochem. 2007;45(8):596–606. https://doi.org/10.1016/j.plaphy.2007.05.002.
Christensen SA, Huffaker A, Kaplan F, Sims J, Ziemann S, Doehlemann G, et al. Maize death acids, 9-lipoxygenase-derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc Natl Acad Sci U S A. 2015;112(36):11407–12. https://doi.org/10.1073/pnas.1511131112.
van Doorn WG. Classes of programmed cell death in plants, compared to those in animals. J Exp Bot. 2011;62(14):4749–61. https://doi.org/10.1093/jxb/err196.
Acknowledgements
The first author thanks China Scholarship Council and Karlsruhe House of Young Scientists for supporting the Ph.D programme. We also sincerely thank Isabelle Meusnier for her excellent technical assistance.
Funding
Open Access funding enabled and organized by Projekt DEAL. This research was supported by a fellowship to JM from the China Scholarship Council and the Karlsruhe House of Young Scientists.
Author information
Authors and Affiliations
Contributions
JBM and MR conceived and designed research. JM conducted experiments. JM and PN analysed data. JM and PN wrote the manuscript. All authors read and approved the manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
All experimental research, including the collection of plant material and work with pathogenic fungus M. oryzae, complies with relevant institutional, national, and international guidelines and legislation.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Additional file 1: Table S1.
Primers used in this experiment for real-time PCR. Fig. S1. Representative images to illustrate the staging system used to classify the colonisation of plant tissue by including both strains GY11-EV and GY11-Avrpia. a-e. without host HR response; f-h. with host HR response. a. Spore without germination. b. Spore with germination; c. Spore with germ tube and appressorium formation but without host HR response; d. Invasive hyphae in one cell without host HR response; e. Invasive hyphae in multiple cells without host HR response; f. Spore with germ tube and appressorium formation with host HR response; g. Invasive hyphae in one cell with host HR response; h. Invasive hyphae in multiple cells with host HR response. Sp spore; Ap appressorium; IH invasive hypha; SGA germinated spore with appressorium; IHO invasive hyphae in one cell; IHM invasive hyphae in multiple cells. Fig. S2. Steady-state transcript levels for genes of jasmonate biosynthesis, jasmonate signalling, defence, and phenylpropanoid metabolism in response to 200 μM of MeJA, scored 24 hours after the onset of the treatment in leaves of the wildtype. The heat map shows the fold-induction over the mock treatment. Fig. S3. Hierarchical clustering of the transcript levels for genes of jasmonate biosynthesis (a), signalling (b), both jasmonate biosynthesis and signalling (c), defence (d), phenylpropanoid metabolism (e), OsAOS2 + OsJAZ9 + defence+phenylpropanoid metabolism (f) and all tested genes (g) in response to mock treatment, or inoculation with the compatible strain GY11-EV, or the incompatible strain GY11-AvrPia in WT and the two jasmonate biosynthesis mutants. Note: numbers for the heatmap row names represent the following: 1: 2 dpi-mock-WT, 2: 2 dpi-mock-cpm2, 3: 2 dpi-mock-hebiba, 4: 2 dpi-GY11-WT, 5: 2 dpi-GY11-cpm2, 6: 2 dpi-GY11-hebiba, 7: 2 dpi-GY11-AvrPia-WT, 8: 2 dpi-GY11-AvrPia-cpm2, 9: 2 dpi-GY11-AvrPia-hebiba, 10: 3 dpi-mock-WT, 11: 3 dpi-mock-cpm2, 12: 3 dpi-mock-hebiba, 13: 3 dpi-GY11-WT, 14: 3 dpi-GY11-cpm2, 15: 3 dpi-GY11-hebiba, 16: 3 dpi-GY11-AvrPia-WT, 17: 3 dpi-GY11-AvrPia-cpm2, 18: 3 dpi-GY11-AvrPia-hebiba.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Ma, J., Morel, JB., Riemann, M. et al. Jasmonic acid contributes to rice resistance against Magnaporthe oryzae. BMC Plant Biol 22, 601 (2022). https://doi.org/10.1186/s12870-022-03948-4
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s12870-022-03948-4