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Between allopatry and secondary contact: differentiation and hybridization among three sympatric Gentiana species in the Qinghai-Tibet Plateau
BMC Plant Biology volume 22, Article number: 504 (2022)
Mountains of the world host a significant portion of all terrestrial biodiversity, and the region of the Qinghai-Tibet Plateau (QTP) stands as one of the most remarkable mountain regions on Earth. Because many explosive radiations occurred there, the QTP is a natural laboratory which is ideal to investigate patterns and processes linked to speciation and diversification. Indeed, understanding how closely related and sympatric species diverged is vital to explore drivers fostering speciation, a topic only rarely investigated in the QTP. By combining genomic and environmental data, we explored the speciation process among three closely related and sympatric species, Gentiana hexaphylla, G. lawrencei and G. veitchiorum in the QTP region.
Combining genome sizes and cytological data, our results showed that G. hexaphylla and G. veitchiorum are diploid, whereas G. lawrencei is tetraploid. Genetic clustering and phylogenetic reconstruction based on genomic SNPs indicated a clear divergence among the three species. Bayesian clustering, migrant, and D-statistic analyses all showed an obvious signature of hybridization among the three species, in particular between G. lawrencei and both G. hexaphylla and G. veitchiorum in almost all populations. Environmental variables related to precipitation and particularly temperature showed significant differences among the three gentians, and in fact a redundancy analysis confirmed that temperature and precipitation were the major climatic factors explaining the genetic differentiation among the three species.
Our study suggested that ancient hybridization, polyploidization, geological isolation and the evolution of different climatic preferences were all likely to be involved in the divergence of the three Gentiana species, as may be the case for many other taxa in the QTP region.
Several major mountain systems of the world are remarkable centres of species diversity, and many of them are classified as hotpots of biodiversity [1,2,3,4]. The accumulation of biodiversity in mountains, over evolutionary times, is usually associated with geological processes (e.g., uplift, erosion) and climatic changes (e.g., climate fluctuations) because they combine to generate a complex topography on which a plethora of highly heterogeneous environments co-exist along altitudinal gradients [2, 5, 6]. This heterogeneity fosters for example divergent natural selection and adaptive radiation [7, 8], which were shown to partly explain the uneven distribution of biodiversity in species richness on Earth [9, 10]. Furthermore, diversification may be promoted by climate-driven cycles modifications of distribution ranges, via a so-called species pump effect . Indeed, depending on the climate state (e.g., Last Inter Glacial (LIG) or Last Glacial Maximum (LGM)), mountain ranges may act either as barriers or facilitators of dispersal, causing distribution ranges of organisms to alternate between fragmentation (isolation; allopatric speciation) [2, 11] and expansion (leading to secondary contact; hybridization) [12, 13].
The highest and largest mountain region of the world, the Qinghai-Tibet Plateau (QTP) region, which includes the QTP platform, the Himalayas and particularly the Hengduan Mountains (HM) , harbours a rich and probably old alpine flora . The HM, also known as the hotspot of biodiversity “Mountains of Southwest China”, are characterized by deep valleys and a warm and seasonally wet climate [16, 17], while the QTP platform is uniformly high and less rugged with a dry climate. It has been showed that the QTP is home to different species assemblages - or motifs - than the HM . Each species of these assemblages is expected to react individually to climate modifications according to its own ecological preferences. Therefore, numerous case studies are needed to unveil and document the most common phylogeographical patterns as well as processes leading up to speciation and diversification in this topographically and climatically dynamic area. Several studies have tackled the phylogeography of a suite of QTP organisms (e.g., [19, 20]), but this necessary work is still ongoing (e.g., reviewed in Muellner-Riehl ).
Geological and climatic dynamics are viewed as major factors fostering intra-specific divergence, speciation and ultimately diversification [14, 21], and only little is known about how biological processes (e.g., adaptation to environment, hybridization) contributed to shape biodiversity in the highly heterogenous environment of the QTP region. For example, in Circaeaster agrestis, isolation both by distance and by local adaptation (via adaptive loci related to stress resistance) have been found , and in Pinus densata, isolation-by-environment explained a significant portion of the genetic structure of the species . These two examples highlight the role of environmental heterogeneity in shaping genetic structure. It was also shown that the molecular signatures of adaptive divergence were similar across two closely related genera in Betulaceae , hinting at a parallel effect of habitat heterogeneity on the evolution of reproductive isolation and speciation. Furthermore, a few studies pointed out the crucial role of hybridization in shaping the flora of the QTP region , but overall, its extent is probably vastly underestimated there . For example, in the genus Saxifraga, the occurrence of hybridization has been reported numerous times in all European clades, whereas it remains almost undetected in the QTP region  despite the much larger number of species in the latter area.
The alpine biome of the QTP region is the centre of diversity and the biogeographical origin of many species-rich taxa, including Gentiana (Gentianaceae) which is distributed in almost all temperate areas of the world [27, 28]. Gentiana is composed of 13 sections , some of which being endemic or near endemic to the region of the QTP. This is for example the case of G. section Kudoa (Masam.) Satake & Toyok. ex Toyok with 25 out of 26 species occurring there [27, 29]. Section Kudoa continuously attracted biologists’ attention due to its horticultural and medicinal value , its taxonomic complexity  and its relevance for evolutionary studies . Previous phylogenetic studies showed a relatively rapid diversification of section Kudoa since the Pliocene [30, 31]. Although ploidy levels are not known for all species in this section, five of them are known to be diploids and two are tetraploids [32,33,34,35]. In this study, we focused on three closely related species in G. section Kudoa, namely G. hexaphylla Maxim., G. veitchiorum Hemsl. and G. lawrencei Burkill of which distribution ranges are largely sympatric (Fig. 1). The three gentians are used in traditional Chinese and Tibetan Medicine and domesticated for horticultural use. The three species can be distinguished by morphological traits such as the shape and arrangement of their leaves (e.g., opposite or in whorls), and the color and shape of their corolla . All three species are perennials and characterized by little pre-zygotic isolation with most visitations being from generalists such as bumblebees [37; personal observations]. Spatial genetic structures were investigated in all three species, unveiling a north-western and south-eastern clade in both G. veitchiorum and G. lawrencei , as well as a northern and southern clade in G. hexaphylla . These spatial genetic structures at least partly derive from a combination of climate-driven range displacement and geological barriers [19, 38], but it remains unclear whether their respective environmental preferences also contributed to the genetic patterns observed. In addition, at least one clade of G. lawrencei is likely to have experienced hybridization with G. veitchiorum in one refugium shared between the two species . Indeed, as in Saxifraga, hybridization may be more common than previously thought in the QTP region for Gentiana [39,40,41] given the number of cases of hybridizationdetected in Europe [e.g., 25, 42, 43] and the much larger number of closely-related species in the QTP region .
Here, to better understand the mechanisms at work in the process of speciation in Gentiana, we combined genomic and climatic data to detect the factors that may have contributed to the divergence among G. hexaphylla, G. veitchiorum and G. lawrencei by sampling across known and spatially structured populations. We specifically aimed to answer (1) Did geographical isolation and climate preferences foster differentiation among the three Gentiana species? (2) How did hybridization affect their divergence?
For genome size estimation, four replications were performed for G. hexaphylla and G. veitchiorum, and three for G. lawrencei. The mean values of the genome size of G. hexaphylla, G. veitchiorum and G. lawrencei were 3.18 G, 3.25 G and 5.00 G, respectively. The standard deviation in the three species ranged from 0.058 to 0.100 (Table S1).
Data preprocessing and SNP calling
Individuals of G. veitchiorum and G. lawrencei were newly sequenced in this study, and the raw data of 35 individuals of G. hexaphylla were retrieved from Fu et al. . After quality filtering, the number of reads retained per sample varied from 4.45 × 106 to 3.18 × 107, with a median value of 1.04 × 107 (Table S2). The depth per sample varied from 5.76 × to 26.97 ×, averaged at 12.53 ×. After filtering for MAF, linkage-disequilibrium (LD) and missing data, the total number of unlinked SNPs obtained for all samples was 143,611. When the outgroup was included, 144,402 SNPs were kept for downstream analysis.
Population genetic structure and genetic divergence
Genomic SNPs showed that G. lawrencei had a slightly higher genetic diversity (e.g., Ar, Ho) than G. hexaphylla and G. veitchiorum (Table 1). The mean Ar vs. Ho in G. lawrencei, G. hexaphylla and G. veitchiorum were 1.1839 vs. 0.1626, 1.1633 vs. 0.1545 and 1.1542 vs. 0.1228, respectively. The Mantel test showed no-significant negetive correlations between altitude and Ar (r2 = 0.042, p = 0.402) and Hoo (r2 = 0.042, p = 0.399), respectively. The CV errors from Admixture analyses showed lowest value at K = 3 (Fig. S1), indicating the three species shall be clustering into three groups, so the inferred three genetic clusters corresponded to the three species included in this study (Fig. 2B). Based on the clustering analyses, introgression was detected from G. lawrencei to G. hexaphylla and G. veitchiorum, and from G. veitchiorum to G. lawrencei. One individual in a population (Fu2016087) of G. hexaphylla contained almost equal genetic composition of G. hexaphylla and G. lawrencei, respectively. From the PCA plot, the first principal component (PC1), which explained 20.46% of all genetic variance, differentiated the three species; the second principal component (PC2), which explained 13.25% of all genetic variance, differentiated the three species as well (Fig. 2 A). Generally, the pairwise FST values were much higher among species than within species (Fig. 3 A). Detailed pairwise FST values between populations were showed in Table S3. The weighted FST values between G. hexaphylla and G. veitchiorum, G. hexaphylla and G. lawrencei, and G. veitchiorum and G. lawrencei were 0.237, 0.187, 0.149, respectively. Plotting FST/(1-FST) and geographic distances among populations in the three Gentiana species showed a significant positive correlation in both within species (r2 = 0.624, p < 2.2e− 16) and between species (r2 = 0.089, p = 0.0007) (Fig. 3B).
Phylogenetic relationship and hybridization among species
Genomic SNPs data resulted in a well-supported tree (Fig. 4). In general, samples clustered together according to the species they were attributed to, except for one population (Fu2016070) of G. lawrencei, which was sister to G. veitchiorum. Gentiana hexaphylla diverged first, and thus was the sister lineages to both G. lawrencei and G. veitchiorum (Fig. 4).
Patterson’s D-statistic revealed strong signals of introgression between G. lawrencei and the other two Gentiana species (Fig. 5). The DBBAA values between G. lawrencei and G. hexaphylla showed that introgression was detected in most population pairs between the two species with p < 1 × 10− 6 (below a Bonferroni-adjusted P-value threshold of 0.001). The signal of introgression between most population pairs was also detected in populations of G. lawrencei and G. veitchiorum (Fig. 5). Week signal of introgression was observed between G. hexaphylla and G. veitchiorum (Fig. 5).
Relationship between genomic differentiation and environmental variables
When all the 19 climate variables were analyzed, 16 out of 19 variables (except bio5, bio10 and bio14) showed significant differences between at least two of the three gentians (Fig. 6). The 16 variables could be grouped into two categories related to temperature (bio1–bio4, bio6-bio9, bio11) and precipitation (bio12–bio13, bio15–bio19). Gentiana lawrencei showed significant differences with the other two gentians in bio1, bio4, bio6–bio9, bio11, bio12, bio17 and bio19. One variable (bio12; Annual Precipitation) showed significant difference among the three gentians, namely G. hexaphylla, G. veitchiorum and G. lawrencei preferred higher, intermediate, and relatively lower annual mean precipitation, respectively (Fig. 6).
After the Pearson correlation analysis, 10 climatic variables (bio1-bio6, bio12, bio14, bio15, bio17) were kept for the analysis. The results of RDA analysis showed that the 10 combined bioclimatic variables had variables contribution in the first two principal components (Fig. 7). Seven variables, bio2 (Mean Diurnal Range), bio3 (Isothermality), bio4 (Temperature Seasonality), bio6 (Min Temperature of Coldest Month), bio14 (Precipitation of Driest Month), bio15 (Precipitation Seasonality) and bio17 (Precipitation of Driest Quarter) had a more substantial contribution on the first principal component (PC1, 25.06%), and the remaining variables on the second principal component (PC2, 15.05%). Among the variables, bio3, bio4, bio6, bio12 (Annual Precipitation), bio14, bio15 and bio17 were significantly differed (P < 0.05, Table 2). Gentiana hexaphylla differed with G. veitchiorum and G. lawrencei along PC1, and the latter two species differed along PC2. For G. hexaphylla and the another two gentians, the variables that explained this differentiation were related to temperature and precipitation in months outside of the growing season, for example bio6 (Min Temperature of Coldest Month), bio14 (Precipitation of Driest Month) and bio17 (Precipitation of Driest Quarter) (Fig. 7). For G. veitchiorum and G. lawrencei, the variables that explained the differentiation were mainly related to bio5 (Max Temperature of Warmest Month).
Genetic divergence among the threeGentianaspecies.
By sampling G. hexaphylla, G. veitchiorum and G. lawrencei across populations known to be spatially structured and thus building upon previous studies [19, 38, 44], our genomic data showed clear genetic divergence among the three sympatric gentians (Fig. 2). The three species are distinct genetic entities, as supported by several morphological traits such as opposite or whorl leaves, shape of leaves, corolla and calyx lobes, as well as corolla color [27, 36]. Nevertheless, the differentiation among the three species was not very strong as the FST value between species ranged from 0.149 to 0.243. We observed that the genetic divergence within species was sometimes larger than that between species and was sometimes associated with geographical distances (Fig. 3). Interestingly, a weak but significant positive correlation was detected between interspecific genetic distance and geological distance (Fig. 3B), rather than the expected negative correlation [45, 46]. This shows the impact of geographical scale on interspecific divergence in the three gentians. Our findings suggest that both physical barriers and heterogeneous environments may have caused isolation and strengthened differentiation among the three Gentiana species. Indeed, previous studies showed that intra-species genetic geographical patterns in G. veitchiorum and G. lawrencei were shaped by isolation in a southeastern and a northwestern refugia [19, 44], whereas the pattern in G. hexaphylla was mainly shaped by geological features at the center of the HM . Genomic data showed that adaption to heterogeneous environments in the QTP region could have produced a high intraspecific divergence [FST=0.89, 20] and a parallel adaptive divergence in a number plants taxa . Therefore, we suggest that geographical isolation as well as adaption to heterogeneous environments could have fostered the differentiation among the three Gentiana species, and thus had a profound impact in their divergence.
Ancient hybridization and polyploidization versus divergence
With a remarkable number of radiations occurring in the alpine biome of the region of the QTP , of which many closely related species are sympatric, it is now crucial to gather more evidence on the role of hybridization and introgression for adaptation, speciation and ultimately diversification. Our analyses showed obvious gene flow among the three gentians (Figs. 2 and 5), which should come as no surprise since hybridization appears to be more common than previously thought in Gentiana. Indeed, it was observed in at least two regions of the world, including the QTP region [e.g., 38–40] and Europe (listed in Favre et al. ), as well as in different sections of Gentiana (e.g., sect. Ciminalis, sect. Gentiana, sect. Cruciata, etc.). A more frequent occurrence of hybridization in Gentiana would also explain some major challenges encountered in taxonomical work and species identification within the genus, as for example in section Chondrophyllae. This is also the case for several taxa of G. section Kudoa, where continuous values for some morphological diagnostic traits were observed [27, 36]. In this section, hybridization may even occur among more species, in this case suggesting a reticulate evolution in its infancy.
In our study, signatures of gene flow were detected especially between G. lawrencei towards both G. hexaphylla and G. veitchiorum in almost all populations (Fig. 5), indicating that their history of hybridization could date back to the origin of these species. Supporting this fact is for example the higher genetic diversity index in G. lawrencei, and the lower FST value between G. lawrencei and the other two species. In fact, previous studies have already detected some cases of hybridization between G. lawrencei and G. veitchiorum in a refugium shared by the two species . Furthermore, species distribution models showed that the three species have had a large overlap of potential distribution through time, which increased since the LIG [19, 38], rendering hybridization and introgression progressively more likely in the last few thousand years.
Hybridization may be followed by allopolyploidization, sometimes setting up the base for the emergence of new taxa, as seen for example in birch trees . Whether this has been the case in section Kudoa or elsewhere in the genus is still difficult to verify, but it is possible since 30.43% of Gentiana species (28 out of 92) for which karyological data are available are in fact polyploids. For the hybridizing species investigated here, karyological studies showed that G. veitchiorum is diploid (2n = 2x = 24, [32, 34]), whereas G. lawrencei is tetraploid (2n = 4x = 48, [33, 34]). In this study, we estimated that the respective genome sizes of G. veitchiorum, G. lawrencei and G. hexaphylla are 3.25 G, 5.00 G and 3.18 G. These results thus suggest that G. hexaphylla – of which ploidy level is still technically unknown - is likely to be a diploid, possibly with ca. 24 chromosomes. Including G. hexaphylla, two out of six species in section Kudoa are tetraploids [32,33,34,35]. Taking their background of hybridization into account, it is not impossible that allopolyploidization participated in the divergence of the three Kudoa species investigated in this study. Yet, a more complete investigation on hybridization and allopolyploidization including all species of that section may be needed.
Did climatic preferences contribute to divergence?
When comparing climatic preferences of G. hexaphylla, G. veitchiorum and G. lawrencei, we found significant differences regarding some components of precipitation and temperature. For example, G. hexaphylla and G. veitchiorum appear to occur in areas with more precipitation, higher temperature, and smaller temperature annual range than G. lawrencei (Fig. 6). In particular, G. hexaphylla prefers habitats with more precipitation than the other two species, consistent with its range limited to the HM, which are characterized by a wetter climate than other areas in the QTP region. Our phylogenetic analysis showed that G. veitchiorum and G. lawrencei diverged from G. hexaphylla (Fig. 4), and that divergence among G. hexaphylla, G. veitchiorum and G. lawrencei occurred in the Pleistocene , in parallel with the local climate becoming progressively cooler and drier [15, 48, 49]. These results showed that divergence among the three gentians shall be correlated to the evolution of climate preferences and corresponding habitat shifts under past climate modifications.
When associating environmental variables with genetic structure to evaluate the impact of environmental heterogeneity on genetic divergence in the three gentians, we indeed found association between species divergence and environmental variables. In this study, we found a significant association between genetic variation and temperature variables (bio3, bio4, bio6) as well as precipitation (bio14, bio15, bio17) (Fig. 7; Table 2). The results suggested that temperature and precipitation were the important drivers of genetic variation among G. hexaphylla, G. veitchiorum and G. lawrencei, as detected in several plants in different continents [20, 50, 51]. Due to elevation-dependent warming, high-mountain environments experienced more rapid changes in temperature than environments at lower elevations . Therefore, considering the ongoing climate warming, temperature appears to be a key driver for adaptation of the three gentians in the future. Studies about trees in the QTP region showed that altitude, rather than other environmental factors, was the key factor affecting genetic diversity (e.g. [53, 54]), but it is not the scenario we observe in the three herbaceous gentians, although a limited number of populations were tested in this study. Finally, since our analysis did not include other environmental variables, such as soil characteristics, composition and structure, we cannot rule out that habitat adaptation to these environmental variables played a significant role in promoting genetic divergence in the three gentians, as was probably the case in European gentians . Considering soil characteristics would now be necessary to investigate speciation and diversification further in the QTP region, especially since rudimentary knowledge on edaphic preferences of QTP species remains vastly unavailable in Gentiana and many other genera.
Materials and methods
Studying species and sampling
We chose six, five and eight populations of Gentiana hexaphylla, G. veitchiorum and G. lawrencei, respectively, to represent their ranges and the main genetic clades found in two earlier studies [19, 38]. In total, this study included 35, 24 and 42 individuals of G. hexaphylla, G. veitchiorum and G. lawrencei, respectively. Information about geographical locations and voucher specimens are list in Table 1. All voucher specimens were deposited in the herbarium of Luoyang Normal University.
Genome size estimation
We used flow cytometry to estimate the genome size of each of the target species using dry material and following the procedure described in Doležel et al. . We used a LB01 lysis buffer to isolate the nuclei for the dried material. One individual of each species was measured three to four times in order to estimate the genome sizes. The cotton (Gossypium hirsutum, TM-1, 2.5 G) or tobacco (Nicotiana tabacum, Yanyan 97, 4.5 G) were used as standards.
Library construction, sequencing and SNP calling
Total genomic DNA was extracted from dry leaves using a Dzup plant genomic DNA extraction kit (Sangon, Shanghai, China). For RAD library construction and sequencing , each sample was digested with the restriction enzyme EcoRI, followed by ligation, purification and size selection as described in Fu et al. . Paired-end reads 150 bp in length were generated using the Illumina Novaseq 6000 (Tianjin, China). Raw reads were filtered and trimmed with Trimmomatic v0.32  with default parameters to remove adaptor sequences and low-quality reads and sites, and then checked for quality with FastQC v0.11.2.
Since RAD-seq study employing reference-based approaches was recommended , we mapped the raw reads against the chromosome-level genome of Gentiana dahurica (PRJNA799480; ), the closest available genome, with bwa-men v2.2.1 , and produced the sequence alignment/map format files with SAMtools v1.9 . We marked the PCR duplications with sambamba v0.8.1 , and called variations with freebayes v0.9.21  with default parameters. Only SNPs were retained in vcftools 0.1.13  for downstream analysis. SNPs with a minor allele frequency (MAF, less than 5%) and missing frequency of more than 0.8 among individuals were removed using vcftools v0.1.13 . Linkage-disequilibrium SNP pruning was performed in vcftools to excludes variants from each pair closer than 100 bp. We used PGDSpider v18.104.22.168  to convert the final VCF file into different formats to perform further analyses.
Genetic diversity and population genetic structure
We computed allelic richness (Ar), observed heterozygosity (HO), gene diveristy (HS), and Wright’s inbreeding coefficient (FIS) using the R package hierfstat . To assess the levels of genetic differentiation among populations, we estimated pairwise FST among populations using the Weir and Cockerham method  in R package hierfstat . A Mantel test was performed in R v. 4.0.1  to check the correlation between genetic diversity (Ar and HO) and altitude in each species. The pairwise FST was graphically displayed with package pheatmap (https://cran.r-project.org/web/packages/pheatmap/) using R. We plotted FST/(1-FST) against pairwise geographic distances among populations to illustrate the range-wide isolation by distance (IBD) pattern in R.
For exploring the genetic clusters, we used a Bayesian clustering method implemented in Admixture  based on the identified SNPs, with assumed clusters (K) from 1 to 20. The cross-validation (CV) procedure performed 10-fold CV (--cv = 10). The CV errors were plotted in R to assess the model complexity for the data. Graphical representation of individual cluster assignments was performed using DISTRUCT v1.1 . The same data set was used to perform a principal component analysis (PCA), with ten main principal components (PCs) extracted in PLINK v1.90 , and visualized using R.
We constructed a phylogenetic tree based on the genomic SNPs using maximum likelihood (ML) in IQ-TREE v.1.6.8  with 1000 ultrafast bootstraps . The best-fitted substitution model was chosen in ModelFinder v2 . The Python script ‘vcf2phylip’ (https://github.com/edgardomortiz/vcf2phylip) was used to transfer the SNPs data for tree building. Gentiana waltonii (specimen no. Fu2020030) was served as the outgroup.
We tested for introgression among the three Gentiana species using Patterson’s D-statistic . The D-statistic uses asymmetry in gene tree topologies to quantify introgression between either of two lineages which share a common ancestor (P1 and P2) and one other lineage (P3) that diverged from the common ancestor of P1 and P2 earlier. We calculated Patterson’s D-statistic for all possible population trios using the Dtrios function of Dsuite v0.5.r44  with default parameters. We fixed G. waltonii as the outgroup. We assessed significance of each test using 100 jackknife resampling runs, and visualized the D-statistic estimates in R.
Environmental data analysis
We obtained 19 climate variables based on the average values from 1950 to 2000 for G. hexaphylla, G. veitchiorum and G. lawrencei from WorldClim (https://www.worldclim.org) using the R package raster  at 30 arc-second resolutions. Significant differences among the three species for these environmental factors were visualized in R.
Highly correlated variables (Pearson’s correlation coefficient > 0.8, p < 0.01) were detected in SPSS and removed to reduce the number of predictors. We used the function rda from the R package vegan  to perform the redundancy analysis (RDA) in order to identify potential environmental factors driving genomic differentiation. We used the function anova.cca from the R package vegan to check the significance of each predictor.
The dataset supporting the conclusions of this article is available in the Figshare (doi: https://doi.org/10.6084/m9.figshare.20445879). The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive  in National Genomics Data Center , China National Center for Bioinformation/Beijing Institute of Genomics, Chinese Academy of Sciences (GSA: CRA008165) that are publicly accessible at https://ngdc.cncb.ac.cn/gsa.
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We are grateful to Zhizhong Li of Wuhan Botanical Garden, Chinese Academy of Sciences for help in data analysis, and to Shulin Zhang of Henan University to offer cotton seeds in genome size estimation.
This work was financially supported by the Foundation of Educational Commission of Henan Province [22A180024] to S.S, the National Natural Science Foundation of China  to P.F.
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We comply with the IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora and confirm that all methods were performed in accordance with the relevant guidelines/regulations/legislation. These wild species samples in this study have not been included in the list of key protected plants. The sampling of wild species in this study was met local policy requirements and did not affect the survival or reproduction of the species. The planting material were identified by Dr. Pengcheng Fu. Voucher specimens were deposited in the herbarium of Luoyang Normal University.
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Fu, P., Favre, A., Wang, R. et al. Between allopatry and secondary contact: differentiation and hybridization among three sympatric Gentiana species in the Qinghai-Tibet Plateau. BMC Plant Biol 22, 504 (2022). https://doi.org/10.1186/s12870-022-03879-0
- Climatic preference
- Genomic SNPs