Skip to main content
Download PDF

Meta-QTL analysis explores the key genes, especially hormone related genes, involved in the regulation of grain water content and grain dehydration rate in maize

BMC Plant Biology volume 22, Article number: 346 (2022) Cite this article

Abstract

Background

Low grain water content (GWC) at harvest of maize (Zea mays L.) is essential for mechanical harvesting, transportation and storage. Grain drying rate (GDR) is a key determinant of GWC. Many quantitative trait locus (QTLs) related to GDR and GWC have been reported, however, the confidence interval (CI) of these QTLs are too large and few QTLs has been fine-mapped or even been cloned. Meta-QTL (MQTL) analysis is an effective method to integrate QTLs information in independent populations, which helps to understand the genetic structure of quantitative traits.

Results

In this study, MQTL analysis was performed using 282 QTLs from 25 experiments related GDR and GWC. Totally, 11 and 34 MQTLs were found to be associated with GDR and GWC, respectively. The average CI of GDR and GWC MQTLs was 24.44 and 22.13 cM which reduced the 57 and 65% compared to the average QTL interval for initial GDR and GWC QTL, respectively. Finally, 1494 and 5011 candidate genes related to GDR and GWC were identified in MQTL intervals, respectively. Among these genes, there are 48 genes related to hormone metabolism.

Conclusions

Our studies combined traditional QTL analyses, genome-wide association study and RNA-seq to analysis major locus for regulating GWC in maize.

Peer Review reports

Background

Maize (Zea mays L.) is one of the most important crops in the world and serves as an important source of feed, food, energy and industrial raw materials. However, the excessively high grain water content (GWC) during maize harvest severely limits the efficiency of mechanized harvesting and increases the cost of drying and storage in China [1]. Especially in the Huang-Huai-Hai region of China, farmers usually delay harvesting to reduce GWC. The measure delays the planting time of the next crops, and greatly affects the annual yield [2]. Grain drying rate (GDR) is a key determinant of GWC [3]. Therefore, increasing GDR and reducing GWC at harvest have become the significant goals of modern maize breeding. The GWC at maturity in maize was mainly due to genetic factors [4], although it is also influenced by environmental factors [4]. Consequently, increasing GDR and reducing GWC from a genetic point of view are of great significance for increasing the maize yield in Wheat-Maize Rotation System of Huang-Huai-Hai region.

Dehydration of maize grains is a complex process involving many physiological and biochemical changes. Studies have shown that maize varieties with fast GDR have faster filling speed. The variation of GWC and GDR has significant changes in the expression of genes related starch biosynthesis [5]. In addition, the growth and development of maize grains is regulated by a variety of plant hormones. The dehydration process of maize grains was likely to be affected by the programmed cell death (PCD) process [6]. Ethylene (ETH) and abscisic acid (ABA) were key regulators of PCD during plant development [7]. ETH treatment could induce early endosperm DNA fragmentation in wheat and maize, while the use of substances that inhibit ETH synthesis, such as 2-aminoethoxyvinylglycine (AVG) treatment, could significantly delay endosperm DNA fragmentation [7]. And ABA can inhibit the occurrence of PCD caused by ETH [7]. The dead cells that had experienced PCD could promote the release of auxin (IAA) [8]. Gibberellin (GA) could also regulate the PCD process of the aleurone layer cells in the grain [9]. Increasing the level of ABA in maize grains had also been shown to accelerate the process of dehydration in the grains [10]. Therefore, many hormones may play a role in the process of grain dehydration. The candidate genes were widely present in the metabolism pathways of various hormones. However, it was still unclear whether there were other hormones and which genes related to hormones played a role in this process.

Since the 1990s, there have been many studies on quantitative trait loci (QTL) mapping related to GDR and GWC [11,12,13,14,15,16]. Sala et al. identified 3 QTLs related GDR and 6 QTLs related GWC through 181 F2:3 one-hybrid families in 2006 [12], accounting for 7.00-14.20% and 10.40-19.70% of the phenotypic variation, respectively [12]. Liu et al. used 232 recombinant inbred lines (RILs) to detect 9 QTLs related GDR in 2010, which accounted for 5.77-13.63% of the total GDR variation [11]. Wang et al. used 280 RILs to identify 14 QTLs related GDR in 2012, and each QTL can explain 5.05-16.28% of the total GDR variation [13]. Li et al. used 258 RILs to detect 35 QTLs related GDR and 40 QTLs related GWC during 4 grain filling stages in 2014 [14]. Li et al. used a recombined inbred population of 242 families to detect 4 QTLs related to GWC in 2019, accounting for 2.19-9.28% of the phenotypic variation [16]. Among them, qGm9-1 overlapped with the QTL9/34 confidence interval (CI) of GWC located by study result of Sala et al. [12], so this QTL may have greater research and utilization value. Up to now, QTLs had been reported on all 10 pairs of maize chromosomes related to GDR and GWC. However, the results of the mapping showed great differences with the different test materials and locations. So far, few of the detected QTLs has been widely recognized by everyone.

In order to make the results of QTL mappings that have been obtained can be efficiently applied to maize breeding practice, we need to summarize and integrate them to screen out QTLs with high effects and small CI. Meta-QTL (MQTL) analysis is a good integrated method. It had been used to estimate the CI of many traits of QTL in many crops [17,18,19,20]. In 2012, Sala et al. summarized 184 QTLs related GWC and performed MQTL analysis on GWC of maize, 34 MQTLs related to GWC were obtained [21]. In the same year, Xiang et al. used 96 QTLs from 12 experiments for a MQTL analysis and 44 MQTLs were identified [17]. However, these two studies only performed MQTL analysis for GWC-related QTLS, and did not perform MQTL analysis for GDR related QTLs. Moreover, in both studies, only GWC-related MQTLs with smaller CI were obtained, but genes in the obtained MQTLs were not analyzed.

As 10 years later, some more literatures about QTL mapping related to GDR and GWC have been published [11, 13, 14, 16]. Compared with the articles related GWC published by Sala et al. and Xiang et al. in 2012, we removed some QTLs that lacked sufficient information, and added 87 QTLs from 4 latest articles [14, 16, 22, 23]. In total, for GWC, we summarized 195 QTLs and performed MQTL analysis. For GDR, we conducted a MQTL analysis on the 87 QTLs from 7 experiments [11,12,13,14,15, 22, 23]. Gene ontology (GO) enrichment analysis and RNA-seq data were also used to analyze the genes in the mapped MQTLs. It was believed that our research results could provide guidance for future research and cloning of genes related to GDR and GWC.

Results

Main features of consensus maps and initial QTLs related GDR and GWC

Twenty-five experiments were found to present QTL and mapping data for GDR and GWC as of December of 2021 (Table 1). Only 14 of those provided sufficient information on mapping and QTL characteristics to carry out map projections and MQTL analysis. For the remain 11 experiments, we could not find their genetic map, but the genetic markers or physical position at both ends of the QTLs mapped to them could be found in the final integrated linkage map, so they also could be used for the following research. The integrated linkage maps associated GDR and GWC were assembled from 14 individual linkage maps and one previously published reference map IBM2 2008 Neighbors (https://www.maizegdb.org/data_center/map). The integrated linkage maps contained 20,301 (related GDR) and 20,376 (related GWC) markers, respectively (Supplementary Data 1). The total length of 2 integrated linkage maps is 8427 cM (related GDR) and 8702 cM (related GWC), respectively (Supplementary Data 1). The densities of the average of all groups for the integrated linkage groups were 2.41 and 2.34 markers per cM for GDR and GWC, respectively (Fig. 1, Supplementary Data 1).

Table 1 Summary of QTL mapping experiments included in MQTL analysis related to GDR and GWC
Full size table
Fig. 1
figure 1

Overview of genome-wide Marker density in the consensus maps related GDR (a) and GWC (b). The ordinate shows the genetic distance along each of the 10 linkage groups corresponding to the maize genome

Full size image

A total of 282 QTLs, including 87 related GDR and 195 related GWC (Fig. 2, Supplementary Data 2), were used to perform the MQTL analysis. The number of initial QTLs for GDR and GWC and their distribution on the maize chromosomes were shown in Fig. 2 and Supplementary Fig. 1a. The results indicated a non-random distribution of QTLs within the maize genome. The individual QTLs were scattered unevenly across chromosomes. For GDR and GWC, chromosome 2 showed the highest number of QTLs (18 QTLs for GDR and 32 QTLs for GWC). Chromosome 10, with 3 QTLs for GDR and 10 QTLs for GWC, had the lowest number of QTLs (Fig. 2, Supplementary Fig. 1a).

Fig. 2
figure 2

Circos diagram for initial QTLs related GDR and GWC. I, colored bars showing the ten maize chromosomes and chro-mosomal positions (cM) presented along the chromosomes. II, distribution of the initial QTLs related GDR on the ten chromosomes of maize (blue). III, proportion of phenotypic variance explained (R2) for each initial QTLs related GDR. IV, distribution of the initial QTLs related GWC on the ten chromosomes of maize (red). V, proportion of phenotypic variance explained (R2) for each initial QTLs related GWC

Full size image

In addition, for GDR, the CI of a single QTL ranged from 4.42 to 295.95 with an average of 56.48 cM (Supplementary Fig. 1b). The phenotypic variation explained (PVE) by a single QTL ranged from 1 to 29% (with an average of 9%), with most of the QTLs exhibiting 5 < PVE ≤ 20% (Supplementary Fig. 1c). For GWC, the CI of a single QTL ranged from 0.74 to 475.76 with an average of 63.67 cM (Supplementary Fig. 1b). The PVE by a single QTL ranged from 1 to 90% (with an average of 14%), with most of the QTLs exhibiting 5 < PVE ≤ 20% (Supplementary Fig. 1c).

MQTL analysis related GDR and GWC

The results of MQTL analysis were listed in Supplementary Table 1. In total, 21 MQTLs related GDR and 47 MQTLs related GWC were identified by means of meta-analysis, each MQTL contained 1 to 16 initial QTLs (Supplementary Table 1).

MQTLs with a large number of initial QTLs detected in multiple environments were considered to be more reliable and stable MQTLs independent of phenotypic environment and genetic background. Therefore, in this study, MQTLs formed from the initial QTLs detected in two or more independent experiments were selected as the final candidate MQTLs. Finally, for GDR, 11 MQTLs were identified (Fig. 3a, Table 2). The average CI of these MQTLs was 24.44 cM, with a maximum and minimum value of 70.84 cM (mGdr2-5 on linkage group 2) and 7.43 cM (mGdr2-3 on linkage group 2), respectively. The MQTL analysis reduced the average interval 57% compared to the average QTL interval for initial QTL (56.48 cM). There were significant differences in the mean CI of multiple QTLs among different chromosomes. For example, the mean CI for MQTLs on chromosome 6 was reduced by 65%, but only reduced by 44% on chromosome 2 (Fig. 3b). 11 MQTLs distributed on chromosomes 2, 3, 4 and 6 with 5, 1, 3 or 2 MQTLs, respectively. The mGdr2-3 with the CI of 7.43 cM on chromosome 2 integrated 6 initial QTLs, the mGdr2-4 with the CI of 13.87 cM on chromosome 2 integrated 9 initial QTLs and mGdr4-2 with the CI of 10.92 cM on chromosome 4 integrated 7 initial QTLs. The remaining 8 MQTLs are all aggregated from less than 5 initial QTLs (Table 2).

Fig. 3
figure 3

Information of MQTLs affecting GDR and GWC. a, positions of MQTLs affecting GDR and GWC in B73 (AGPv4). Dis-tances on the map are in Mb. The MQTLs related to GDR are marked in blue, and those related to GWC are marked in red. b and c, the fold reduction in CI of QTLs after MQTL analysis related GDR (b) and GWC (c). The fold reduction is used here (CI of initial QTLs - CI of MQTLs)/CI of initial QTLs

Full size image
Table 2 Information of GDR and GWC MQTLs we got through MQTL analysis
Full size table

For GWC, in total, 34 MQTLs were formed from the initial QTLs detected in two or more experiments, a reduction of 83% on the number of initial QTL (Fig. 3a, Table 2). The average CI for these MQTLs was 22.13 cM, ranging from 0.74 (mGwc7-3 on linkage group 7) to 91.86 cM (mGwc3-5 on linkage group 3) (Table 2). This is an improvement in resolution of 65% when compared to the average QTL interval for initial QTL (63.67 cM). Similarly, there were significant differences in the mean CI of multiple QTLs among different chromosomes. The mean CI for MQTLs on chromosome 7 was reduced by 91%, but only reduced by 32% on chromosome 9 (Fig. 3c). Compared with 34.1 cM in study result of Xiang et al. [17], the average CI had also been greatly improved. The highest number of MQTLs was 8 which located in linkage group 2, and no MQTLs for GWC were found in linkage group 1.

In order to verify the accuracy of the MQTLs we obtained, we compare our MQTLs results with the research results of Sala et al. [21]. Fourteen overlap-domains were existed between the MQTLs of our obtained and the MQTLs in the study of Sala et al. (Supplementary Table 2). Meanwhile, the accuracy of the these MQTLs we obtained in overlap-domains had been greatly improved. The average CI of these MQTLs we obtained in overlap-domains was 34.04 cM which reduced the average interval 58.48% compared to the average CI for the MQTLs (81.99 cM) that Sala et al. obtained in overlap-domains (Supplementary Table 2). Especially, the mGwc4-2 and S17 were overlapped on 250.2-253.7 cM on chromosome 4. On the basis of S17, the CI of mGwc4-2 was reduced from 118.71 cM to 3.50 cM (Supplementary Table 2). ZmFIE1 (Zm00001d049608) was a gene identified in mGwc4-2. Its homologous gene in Arabidopsis, FIE1, could inhibit the expression of the flowering gene FLC, thereby affecting plant flowering [25]. Changes in plant flowering can lead to changes in plant growth, which can affect grain moisture content at harvest. In addition, the mGwc6-4 and S22 were overlapped on 155.2-161.3 cM on chromosome 6. Relative to the CI of S22 (32.44 cM), the CI of mGwc6-4 was reduced to 6.08 cM. ZmTGL1 (Zm00001d036773) was a gene identified in mGwc6-4. According to the gene annotation on MaizeGDB (https://www.maizegdb.org/), ZmTGL1 catalyzes lipid degradation in maize. Studies showed that lipid content in maize grains was negatively correlated with GDR [26]. ZmTGL1 may affect grain dehydration by affecting the metabolic processes in the grain.

Candidate genes related GDR and GWC identified in MQTL regions

By comparing the positions of the genetic markers at both ends of the MQTL on the B73 genome (AGPv4) [27], a great quantity candidate genes were identified in the selected intervals of MQTLs related GDR and GWC. A total of 1494 candidate genes related to GDR and 5011 candidate genes related to GWC were identified in the current MQTL intervals (Table 2, Supplementary Data 3). We then used GO enrichment analysis to assign candidate genes to functional categories (Fig. 4).

Fig. 4
figure 4

GO enriched in the genes in MQTLs related to GDR (a) and GWC (b). The parameters are P-value ≤0.01

Full size image

The results for biological processes showed that the candidate genes related GDR were enriched in many biological processes related to lipid homeostasis, such as “lipid A metabolic process”, “lipid A biosynthetic process”, “fatty acid oxidation” and so on (Fig. 4). Lipid homeostasis could affect the transportation of nutrients from the maternal placenta to the developing endosperm, thereby affecting the grain filling process [28]. Studies had shown that there was a certain relationship between the grain filling rate and GDR [5]. Therefore, lipid homeostasis may affect the dehydration process of grains by affecting grain filling. Among all 27 candidate genes related lipid homeostasis, 15 genes were expressed in seed (Supplementary Fig. 2). 7 genes were highly expressed before 10 days after pollination (DAP), 1 gene was highly expressed between 10 DAP and 30 DAP, 2 genes were highly expressed after 30 DAP, and 5 genes were highly expressed before 10 DAP and after 30 DAP.

Genes associated with GWC were also enriched in some biological processes related to lipid metabolism. In addition, many genes were associated with energy metabolism and organization development, such as “Nicotinamide adenine dinucleotide (NAD) biosynthetic process” and “organ growth” (Fig. 4). The grain filling process required respiration to provide energy, and NAD was an important product of respiration. The NAD biosynthetic process may affect the dehydration process by affecting grain filling. Among all 9 candidate genes related NAD biosynthetic process, 8 genes were expressed in seed. 5 genes were highly expressed before 10 DAP, 2 genes were highly expressed before 30 DAP, and 1 gene was highly expressed during all seed developmental process (Supplementary Fig. 3). Among all 16 candidate genes related organ growth, 14 genes were expressed in seed. Seven genes were highly expressed before 10 DAP, 1 gene was highly expressed between 10 DAP and 30 DAP, 3 genes were highly expressed after 30 DAP, and 3 genes were highly expressed before 10 DAP and after 30 DAP (Supplementary Fig. 3). This indicated that GWC may be influenced by the process of early grain development, particularly that of the embryo, which required a lot of energy. This factor had been neglected in previous studies on GWC, and the process of grain morphogenesis prior to the dehydration process should also be paid attention to.

MQTL analysis indicated that some MQTLs associated with GDR and GWC were clustered in the same regions of certain chromosomes (Fig. 5a). A total of 323 overlapping candidate genes were identified on both of GDR and GWC MQTL (Supplementary Data 4). Go enrichment analysis showed that there were many genes related to the degradation of polysaccharides and proteins, and some genes were related to the regulation of ion channel protein activity (Supplementary Fig. 4). There was a layer of waxy cuticle on the surface of maize grain, which may influence the water loss in maize grain to some extent. Among all 45 candidate genes related polysaccharides and proteins metabolism, 23 genes were expressed in seed. Eleven genes were highly expressed before 10 DAP, 4 genes were highly expressed between 10 DAP and 30 DAP, 4 genes were highly expressed after 30 DAP, and 4 genes were highly expressed before 10 DAP and after 30 DAP (Fig. 5b). In addition, it also contained 5 genes related to the regulation of ion channel protein activity.

Fig. 5
figure 5

Information of overlap domains between the GDR and GWC-related MQTLs. a, chromosome position of the domain on maize genome (AGPv4) which was GDR and GWC-related MQTLs overlap with 95% CI. The MQTLs related to GDR are marked in blue, the MQTLs related to GWC are marked in red, and the overlap domains between the GDR and GWC-related MQTLs are marked in yellow. b, expression pattern of partial genes contained in domain where GDR and GWC-related MQTLs overlap in seed during 0-38 DAP

Full size image

Comparing these GDR and GWC-related candidate genes with the results of genome-wide association study (GWAS) mapping of GDR and GWC published [4, 29,30,31,32], the results showed that there are 7 GDR-related and 14 GWC-related genes in the candidate genes mapped by GWAS (Supplementary Fig. 5, Supplementary Table 3). According to annotation, 5 of these genes were involved in protein degradation, and Zm00001d007793 in mGdr2-5 and Zm00001d002535 in mGdr2-1 and mGwc2-1 were involved in protein degradation through the E3 ubiquitin ligase pathway. Previous reports had confirmed that part of E3 ubiquitin ligase may be involved in water transfer in plants [33, 34].

Analysis of key hormone candidate genes related GDR and GWC

The growth and development of maize grains was regulated by a variety of plant hormones. In total, 72 genes related to hormones were detected (Supplementary Table 4), and we found 48 of these were expressed in seed [35, 36], including 6 genes related ABA, 9 gene related ETH, 4 genes related IAA, 3 genes related GA, 3 genes related cytokinin (CTK), 6 genes related jasmonic acid (JA), 16 genes related salicylic acid (SA) and 1 gene related strigolactone (SL) (Fig. 6).

Fig. 6
figure 6

The presence of MQTLs related to GDR and GWC in ABA, ETH, IAA, GA, CTK, JA, SA and SL metabolism pathways. The genes related to GDR were marked in blue, and those related to GWC were marked in red. The green genes represented candidate genes that are related to both GDR and GWC

Full size image

ABA had been shown to increase GDR during grain development [37]. We found 2 GDR-related candidate genes and 4 GWC-related candidate genes to ABA metabolism (Fig. 6). ZmMOCS2 (Zm00001d006342) was identified in mGwc2-5, and encoded a molybdenum cofactor sulfurase (MoCo). ZmMOCS2 were expressed highly after 30 DAP (Fig. 6). LOS5, a gene encoded MoCo in Arabidopsis, could regulates the last step of ABA biosynthesis [38]. Previous report showed that overexpressed LOS5 in maize could significantly increase the activity of aldehyde oxidase, which resulted in the accumulation of ABA and improved the drought resistance of maize [38]. The zeaxanthin epoxidase (ZEP) encoded by ZmZEP1 (Zm00001d003512) in mGwc2-2 and Zm00001d017199 in mGwc5-4, 9-cis-epoxycarotenoid dioxygenase (NCED) encoded by ZmNCED4 (Zm00001d007876) in mGdr2-5, xanthoxin dehydrogenase (ABA2) encoded by Zm00001d049277 in mGdr4-1 and abscisic acid 8′-hydroxylase (ABH) encoded by Zm00001d011117 in mGwc8-4 were key enzymes in the process of ABA synthesis and degradation [39]. ZmZEP1 was highly expressed before 6 DAP and after 32 DAP (Fig. 6). ZmZEP5, Zm00001d049277 and Zm00001d011117 were expressed highly before 8 DAP, while ZmNCED4 was highly expressed after 30 DAP (Fig. 6). This showed that ABA in the process of grain dehydration was likely to be degraded mainly through 8′-hydroxylase pathways.

ETH was a key regulator of starch synthesis and PCD during plant development [6]. We found 1 GDR-related candidate genes and 9 GWC-related candidate genes to ETH metabolism (Fig. 6). ZmACS2 (Zm00001d002592) was a gene present in both mGdr2-1 and Gwc2-1. ZmACS2 was highly expressed after 30 DAP (Fig. 6). ZmACS2 and ZmACS3 (Zm00001d045479) in mGwc9-2 encoded a ACC synthase (ACS). ZmACCO5 (Zm00001d011208) in mGwc8-4 and Zm00001d042996 in mGwc3-5 encoded a ACC oxidase (ACO). ACS and ACO were all key enzymes in the process of ethylene synthesis [40]. ZmACS3 was highly expressed after 14 DAP, ZmACCO5 was highly expressed before 8 DAP and Zm00001d042996 was highly expressed before 6 DAP and after 30 DAP (Fig. 6). Moreover, the 5′-methylthioadenosine nuclease (MTN) encoded by ZmMTN1 (Zm00001d049823) in mGwc4-3 and ZmMTN2 (Zm00001d049823) in mGwc9-2, transaminase encoded by ZmTIDP3358 (Zm00001d013992) in mGwc5-1 were both enzymes in the synthesis of ETH. ZmMTN1 and ZmMTN2 were highly expressed before 8 DAP, and ZmTIDP3358 was highly expressed after 12 DAP (Fig. 6). The glutamyltransferase (GGT) encoded by ZmIDP2449 (Zm00001d003446) in mGwc2-2 and jasmonate resistant 1 (JAR1) encoded by ZmAAS11 (Zm00001d009714) in mGwc8-2 could inactivate ACC, the direct precursor of ETH synthesis [41]. Both ZmIDP2449 and ZmAAS11 were mainly expressed before 8 DAP and after 30 DAP (Fig. 6). For ZmAAS11, except for ETH, it also plays an important role in the metabolic pathways of JA which indicates that these hormones may act together in the dehydration process of grains [42].

IAA could regulate plenty of aspects of plant growth and development. Recent studies showed that IAA could promote the expression of starch biosynthesis genes in rice [43]. In addition, IAA could promote plant resistance to environmental stress by controlling the expression of Gretchen Hagen 3 (GH3) and other IAA-responsive family genes [44]. We found 4 GWC-related candidate genes to IAA metabolism (Fig. 6). ZmAas3 (Zm00001d007395) identified in mGdr2-7 and ZmAas7 (Zm00001d043350) identified in mGwc3-5 were genes of the GH3 family. ZmAas3 and ZmAas7 were highly expressed after 30 DAP (Fig. 6). The expression of ZmAas3 in shoots could be induced by sodium chloride treatment, which may be related to the response of maize to water deficit stress [45]. Zm00001d043235 in mGwc3-5 was a gene related IAA decomposition, which was highly expressed before 6 DAP (Fig. 6). ZmDE18 (Zm00001d023718) in mGwc10-1 was a gene in the synthesis of IAA [46], which was highly expressed between 8 DAP and 28 DAP (Fig. 6).

GA was an important type of plant hormone, which played an important role in the process of plant flowering induction and fruit development [47, 48]. We found 1 GDR-related and 2 GWC-related candidate genes to GA biosynthesis [49] (Fig. 6). The ent-kaurenoic acid oxidase (KAO) encoded by ZmD3 (Zm00001d045563) in Gwc9-2, GA13oxidase (GA13Ox) encoded by ZmGA13Ox1 (Zm00001d007180) in mGdr2-4 and GA2oxidase (GA2Ox) encoded by ZmGA2Ox3 (Zm00001d043411) in mGdr3-5. ZmD3 was highly expressed after 20 DAP, ZmGA13Ox1 was highly expressed before 4 DAP and after 38 DAP (Fig. 6). ZmGA2Ox3 was highly expressed before 8 DAP (Fig. 6).

CTK plays a prominent role in promoting cell division, and the CTK in maize grains mainly occurs in the endosperm, and the concentration of CTK in the developing embryo is very low. Therefore, it is speculated that CTK promotes cell division in the endosperm [50]. We found 3 GWC-related candidate genes to CTK metabolism (Fig. 6). Cytokinin-activating enzyme encoded by Zm00001d021450 in mGwc7-4 and Zm00001d011246 in mGwc8-4 was a key enzyme in the synthesis of CTK [51]. The expression levels of these two genes were highly before 4 DAP (Fig. 6). The CTK oxidase (CKO) encoded by ZmCKO4 (Zm00001d043293) in mGwc3-5 could catalyze the degradation of CTK [51], which was highly expressed in the whole grain development stage (Fig. 6).

JA and SA mainly played a role in the defense of plant diseases and insect pests, and few studies had been done on their role in the development of maize grains. It was currently known that SA may antagonize each other with IAA [52] and affected the formation of sugar in maize grains [53]. For JA, 6 genes related GWC were detected (Fig. 6). Of them, the expression of Zm00001d047340 in mGwc9-4 was significantly higher than that of other genes. It encoded an allene oxide cyclase (AOC) and was highly expressed before 8 DAP (Fig. 6). Furthermore, the 16 SA-related candidate genes we detected were all genes related to SA synthesis, and no expressed SA degradation-related genes were detected [54] (Fig. 6). Among them, ZmGOT1 (Zm00001d043382) in mGwc3-5 had the highest expression level, and the expression levels of other SA-related genes were 30 to 40 times higher. Its expression level was high after 10 DAP.

SL played an important role in regulating flowering time of plants, and could regulate flowering time of plants together with melatonin [55]. Previous studies had shown that there was a “safe threshold” for melatonin regulation of flowering in Arabidopsis, within which SL inhibits flowering by inhibiting the expression of SPL gene. Outside the threshold range, strigolactone inhibits flowering by inhibiting melatonin synthesis, which in turn promotes the expression of the flowering suppressor gene FLC [55]. ZmY9 (Zm00001d023655) in mGwc10-1 encoded a 15-cis-ζ-carotene isomerase (Z-ISO) and played a role in the biosynthesis of SL [56]. It was highly expressed after 30 DAP (Fig. 6).

Discussion

Maize is one of the most important crops in the world and an important source of feed, food, energy and industrial raw materials. However, the excessively high GWC during maize harvest in my country severely limits the efficiency of mechanized harvesting and increases the cost of drying and storage [57]. Therefore, breeders regard GDR and GWC at harvest as one of the important indicators of molecular marker-assisted breeding.

Genetic architecture of GDR and GWC revealed by MQTL analysis

To gain a better understanding of the genetic architecture of GDR and GWC, we performed MQTL analysis using reported QTL from previous mapping experiments. In total, 282 QTLs from 25 previous QTL mapping experiments were projected onto the integrated linkage map successfully (Table 1, Supplementary Data 2).

Since the 1990s, there had been many experiments on QTL mapping related to maize grain moisture [12, 15, 22, 24]. These QTL mapping experiments could be divided into two categories: QTL mapping related to GDR and GWC. However, the number of experiments and QTLs related to GWC was far more than them related to GDR. Reasons may be that GDR was more susceptible to environmental conditions than GWC. Undoubtedly, the effectiveness of marker—assisted selection strongly depended on the accuracy of QTL mapping results in terms of position and effects of the detected QTL. Analysis of quantitative trait relationships is important to evaluate the feasibility of joint selection of two or more traits [58, 59]. Previous studies have shown that GDR is a key determinant of GWC [3]. Here, we performed co-localization analysis of GDR and GWC-related MQTLs and found that there were only 3 overlapping regions containing 323 candidate genes. Col-localization of MQTLs for the GDR and GWC is in line with the results of previous studies, showing that the variability in GDR and GWC of maize may be due to distinct genetic architectures [4]. However, many of the 323 genes could affect GWC at harvest in various ways. Therefore, in the process of QTL mapping and marker—assisted selection, we could use the GWC at harvest as an indicator and GDR as a reference to improve the effectiveness of marker—assisted selection to a certain extent [60]. There are many co-localized genes between GDR and GWC, suggesting that there may be some common regulatory mechanisms between these two traits.

The interaction between different QTLs had a greater impact on GWC than a single QTL. Among the results of QTL mapping for GWC, the likelihood of odd (LOD) value of the final QTL was mostly high, but the explanatory variation rate of the phenotype was mostly very low. The maximum explanatory variation rate of the phenotype in many articles was below 10%. Combined with the existing research results, there were significant interactions between QTL and QTL (more effective in the early stage of grain dehydration), and between QTL and the environment (more effective in the late stage of grain dehydration) [4]. These two interactions may have a more significant impact on grain moisture than a single QTL. This indicated that it may be a more effective method to study the dehydration process of seeds from a biological process rather than from genes.

Previous studies had shown that the grain dehydration process may be affected by biological processes such as starch synthesis and PCD [10]. Hormones played an important regulatory role in these two biological processes. ABA and ETH could antagonize and regulate the PCD process of endosperm cells, and increasing the ratio of ABA to ETH had also been shown to improve the grain filling process of rice. The increase in ABA content had been shown to increase GDR and reduce GWC at harvest. Therefore, it was a good choice to study the grain dehydration process from the perspective of hormones. Among the candidate genes in the MQTLs we obtained, 48 genes related to hormone metabolism were detected (Fig. 6). These genes and corresponding hormones had complex interactions in the process of grain development. Studying these 48 genes related to hormone metabolism could help us better understand the process of grain dehydration.

Other influencing factors of GDR and GWC

The dehydration process of grains was affected by many factors [5]. In addition to the starch synthesis and PCD that had been reported before, it was also affected by many other factors. The results of GO enrichment analysis contained many biological processes related to lipid homeostasis, “lipid A metabolic process”, “lipid A biosynthetic process”, “fatty acid oxidation” and so on (Fig. 4), indicating that lipid homeostasis may also have a certain impact on the dehydration process of grains. The large enrichment of genes related to energy metabolism and organization development in GWC-related genes. There were some genes related to early embryo development of grains contained in GWC-related genes, which indicated that the early development of grain, which had been neglected in many previous studies, may also affect grain dehydration. Many genes related to energy metabolism were enriched, which was consistent with the high energy requirement during grain development.

Conclusions

In this study, MQTL analysis was performed using 282 QTLs from 25 studies to propose MQTL on a high-density genetic linkage map. For GDR and GWC, a total of 11 and 34 MQTLs were identified. The MQTLs identified possessed a narrower CI than the original QTLs. with the CI of MQTLs related to GDR and GWC were 24.44 and 22.13 cM, respectively. A total of 1494 candidate genes related to GDR and 5011 candidate genes related to GWC were identified in MQTL intervals. Among these genes, there are 48 genes related to hormone metabolism, indicating that hormones may play an important role in the process of grain dehydration. Of these 48 genes, 45 genes are highly expressed across before 8 DAP or after 30 DAP in grain and many genes are related to the starch synthesis and PCD. In addition, factors such as lipid homeostasis, energy metabolism and organization development may also have a certain impact on the process of grain dehydration. Our results provide new ideas for studying grain dehydration, analyzing major locus for regulating GWC in maize, which will help maize breeding.

Methods

Collection of QTL information related GDR and GWC of maize

All data were collected from 25 experiments on QTLs related GDR and GWC of maize published from 1992 to 2020 (Table 1). Here, we defined that an experiment was as the QTL analysis of one population for a given trait in a given environment. Collected information related to QTL mapping related GDR and GWC of maize in the literatures, including name, size, type and mapping function of mapping population, trait information, name of QTL, location of chromosome, LOD value, phenotypic contribution rate, additive effect and heredity number and location of map markers.

QTL projection and MQTL analysis

Projected the identified QTL onto a reference map for MQTL analysis. The projection was performed using BioMercator (v4.2) [61]. For the experiments with complete genetic map and QTL information, the information was arranged according to the format required by the software. For the experiments that did not give the LOD value, the LOD value was uniformly taken as 2.5 [17]; if only CI (95%) or R2 value of a QTL was known, it could be inferred based on the following formulas:

$$\mathrm{CI}=530\div \left(\mathrm{N}\times {R}^2\right)$$
(1)
$$\mathrm{CI}=163\div \left(\mathrm{N}\times {R}^2\right)$$
(2)

In the two formulas, “CI” was the confidence interval of QTL; “N” represented the size of the mapping population; “R2” represented the genetic contribution rate of QTL; formula (1) applied to backcross and F2 mapping populations [62]; formula (2) applied to recombinant inbred lines mapping populations [63].

For experiments without genetic map but with markers at both ends of QTLs, put the QTLs of it compare to the reference genetic map IBM2 2008 Neighbors (https://www.maizegdb.org/data_center/map). QTLs that could not be compared with the reference genetic map at both ends of the marker was discarded directly. For QTLs that only one segment of marker can be compared to the reference genetic map successfully, after the CI was calculated according to the above formula, the genetic position of the marker on the other end can be calculated according to the genetic position of the marker on the comparison. In the experiments of QTL mapping with SNP markers, SNP markers could not be associated with other genetic maps, so MQTL analysis cannot be performed directly. However, the physical location information of QTL obtained by SNP markers was given. Therefore, I manually converted the physical locations at both ends of QTLs to the corresponding genetic locations on the reference genetic map IBM2 2008 Neighbors, and then conducted MQTL analysis.

The MQTL analysis of GDR and GWC were performed separately. All information related to a single genetic map used in this experiment was collected based on published maps. Take the IBM2 2008 Neighbors high-density genetic linkage map as the reference map. The detailed projection program was executed according to the method of Chardon et al. described [64]. We integrated the genetic maps of literatures and the IBM2 2008 Neighbors reference genetic map to get the consensus map. In a few cases, the display order of common markers was inconsistent (the order between maps). Whenever the removal of the marker would not degrade the construction of the consensus graph, it would be discarded from the analysis. The QTL in their original map was projected onto the consensus map, using markers shared by the two maps through similar functions. According to the location and CI of each QTL, the software tested the akaike type criteria (AIC) values of the AIC model when the number of MQTLs on a specific chromosome was 1-10. When selecting the number of MQTL on each chromosome, the number of MQTL corresponding to AIC minimum was considered first. Then performed meta-analysis again to synthesize MQTL analysis.

In order to facilitate the display of the initial QTLs, the genetic map used in Fig. 2 was a genetic map obtained by integrating the GDR and GWC-related integrated genetic maps using IBM2 2008 Neighbors as the reference genetic map by BioMercator (v4.2).

Acquisition of candidate gene and GO enrichment analysis

After obtaining MQTLs given by the software, MQTLs obtained from at least two experiments were screened out. The physical location of partial markers on B73 (AGPv4) of IBM2 2008 Neighbors could be found on MaizeGDB (https://www.maizegdb.org/) [27], and the physical location of genetic markers closest to both ends of MQTLs was first checked on IBM2 2008 Neighbors. Finally, the precise physical positions at both ends of MQTLs could be obtained by using the following formula [65].

$$\mathrm{p}=\mathrm{p}1+\left(\mathrm{p}2-\mathrm{p}1\right)\times \left(\mathrm{g}-\mathrm{g}1\right)\div \left(\mathrm{g}2-\mathrm{g}1\right)$$

“g1” and “g2” were the genetic locations of bilateral genetic markers, “g” was the genetic location of target genetic markers, “p1” and “p2” were the physical locations of bilateral genetic markers, and “p” was the physical location of target genetic markers.

Finally used the “qTeller” module on MaizeGDB to look up the genes contained in MQTLs as candidate genes.

We used the Agrigo (v2.0) website (http://systemsbiology.cau.edu.cn/agriGOv2/) to perform GO enrichment analysis on candidate genes related GDR and GWC, respectively. When the P-value of the term was less than 0.01, we considered it enriched in the corresponding gene set. For the display of GO enrichment analysis results, we used the form of “bubble chart” to display. The drawing of “bubble chart” was done using the R language package (ggplot2) [66].

RNA-seq data processing and drawing of the chart

For the RNA-seq of maize grains downloaded from the NCBI from 0 to 38 days after pollination [35, 36], we now divided the expression levels at different time points by the maximum observed fragments per kilobase of transcript per million mapped reads (FPKM) to calculate the normalized expression value of the gene. Then used MeV (v4.9) software to draw a heat map of gene expression. The GDR and GWC of the RNA-seq samples was not obtained [35]. According to previous results, the variation trend of GWC of different hybrids that planted at the close planting place and obtained at the similar developmental stage with the RNA-seq samples, was showing a “S”-shaped downward trend of “slow-fast-slow” [67, 68] and the GDR showed a unimodal curve that first increased and then decreased with the increase of silking days [68].

Availability of data and materials

All data supporting the conclusion of this article are provided within the article and its supplementary.

Abbreviations

ABA:

Abscisic acid

ABH:

Abscisic acid 8′-hydroxylase

ACO:

ACC oxidase

ACS:

ACC synthase

AIC:

Akaike type criteria

AOC:

Allene oxide cyclase

AVG:

2-aminoethoxyvinylglycine

CI:

Confidence interval

CKO:

CTK oxidase

CTK:

Cytokinin

DAP:

Days after pollination

ETH:

Ethylene

FPKM:

Fragments per kilobase of transcript per million mapped reads

GA:

Gibberellin

GA2Ox:

GA2oxidase

GA13Ox:

GA13oxidase

GDR:

Grain drying rate

GGT:

Glutamyltransferase

GH3:

Gretchen Hagen 3

GO:

Gene ontology

GWAS:

Genome-wide association study

GWC:

Grain water content

IAA:

Auxin

JA:

Jasmonic acid

JAR1:

Jasmonate resistant 1

KAO:

ent-kaurenoic acid oxidase

LOD:

Likelihood of odd

MoCo:

Molybdenum cofactor sulfurase

MTN:

5′-methylthioadenosine nuclease

MQTL:

Meta-QTL

NAD:

Nicotinamide adenine dinucleotide

NCED:

9-cis-epoxycarotenoid dioxygenase

PCD:

Programmed cell death

PVE:

Phenotypic variation explained

QTL:

Quantitative trait locus

RIL:

Recombinant inbred line

SA:

Salicylic acid

SL:

Strigolactone

ZEP:

Zeaxanthin epoxidase

Z-ISO:

15-cis-ζ-carotene isomerase

References

  1. Chai Z, Wang K, Guo Y. The current situation of mechanical corn harvest quality and its relationship with moisture content. Sci Agric Sin. 2017;50(11):2036–43.

    Google Scholar 

  2. Wang K, Li S. Analysis of factors affecting dehydration rate of maize kernels. Sci Agric Sin. 2017;50(011):2027–35.

    Google Scholar 

  3. Chase SS. Relation of yield and number of days from planting to flowering in early maturity maize hybrids of equivalent grain moisture at harvest. Crop Sci. 1964;4(1):111–2.

    Article  Google Scholar 

  4. Li W, Yu Y, Wang L, Luo Y, Peng Y, Xu Y, et al. The genetic architecture of the dynamic changes in grain moisture in maize. Plant Biotechnol J. 2021;19(6):1195–205.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Qu J, Zhong Y, Ding L, Liu X, Xu S, Guo D, et al. Biosynthesis, structure and functionality of starch granules in maize inbred lines with different kernel dehydration rate. Food Chem. 2022;368:130796–805.

    Article  CAS  PubMed  Google Scholar 

  6. Young TE, Gallie DR. Programmed cell death during endosperm development. Plant Mol Biol. 2000;44(3):283–301.

    Article  CAS  PubMed  Google Scholar 

  7. Young TE, Gallie DR. Regulation of programmed cell death in maize endosperm by abscisic acid. Plant Mol Biol. 2000;42(2):397–414.

    Article  CAS  PubMed  Google Scholar 

  8. Jiang C, Wang J, Leng H, Wang X, Liu Y, Lu H, et al. Transcriptional regulation and signaling of developmental programmed cell death in plants. Front Plant Sci. 2021;12:702928–72935.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Zhang H, Xiao Y, Deng X, Feng H, Li Z, Zhang L, et al. OsVPE3 mediates GA-induced programmed cell death in Rice Aleurone layers via interacting with actin microfilaments. Rice (New York, N.Y.). 2020;13(1):22.

    Google Scholar 

  10. Zhang L, Liang X, Shen S, Yin H, Zhou L, Gao Z, et al. Increasing the abscisic acid level in maize grains induces precocious maturation by accelerating grain filling and dehydration. Plant Growth Regul. 2018;86(1):65–79.

    Article  CAS  Google Scholar 

  11. Liu X, Wang Z, Wang X, Li T, Zhang L. Primary mapping of QTL for dehydration rate of maize kernel after Physiolo- gical maturing. Acta Agron Sin. 2010;36(1):47–52.

    Article  CAS  Google Scholar 

  12. Sala RG, Andrade FH, Camadro EL, Cerono JC. Quantitative trait loci for grain moisture at harvest and field grain drying rate in maize (Zea mays, L.). Theor Appl Genet. 2006;112(3):462–71.

    Article  CAS  PubMed  Google Scholar 

  13. Wang Z, Wang X, Zhang L, Liu X, Di H, Li T, et al. QTL underlying field grain drying rate after physiological maturity in maize (Zea Mays L.). Euphytica. 2012;185(3):521–8.

    Article  Google Scholar 

  14. Li Y, Dong Y, Yang M, Dong Y. QTL detection for grain water relations and genetic correlations with grain matter accumulation at four stages after pollination in maize. J Plant Biochem Physiol. 2014;02(01):1000121–9.

    Google Scholar 

  15. Capelle V, Remoue C, Moreau L, Reyss A, Mahe A, Massonneau A, et al. QTLs and candidate genes for desiccation and abscisic acid content in maize kernels. BMC Plant Biol. 2010;10:2–23.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  16. Li L, Zhang L, Cui M. QTL mapping of traits related to water content in maize kernels. Jiangsu Agric Sci. 2019;47(09):93–6.

    Google Scholar 

  17. Xiang K, Reid LM, Zhang Z, Zhu X, Pan G. Characterization of correlation between grain moisture and ear rot resistance in maize by QTL meta-analysis. Euphytica. 2012;183(2):185–95.

    Article  CAS  Google Scholar 

  18. Liu Y, Salsman E, Wang R, Galagedara N, Zhang Q, Fiedler JD, et al. Meta-QTL analysis of tan spot resistance in wheat. Theor Appl Genet. 2020;133(8):2363–75.

    Article  CAS  PubMed  Google Scholar 

  19. Izquierdo P, Astudillo C, Blair MW, Iqbal AM, Raatz B, Cichy KA. Meta-QTL analysis of seed iron and zinc concentration and content in common bean (Phaseolus vulgaris L.). Theor Appl Genet. 2018;131(8):1645–58.

    Article  CAS  PubMed  Google Scholar 

  20. Daryani P, Darzi Ramandi H, Dezhsetan S, Mirdar Mansuri R, Hosseini Salekdeh G, Shobbar Z. Pinpointing genomic regions associated with root system architecture in rice through an integrative meta-analysis approach. Theor Appl Genet. 2022;135(1):81–106.

    Article  CAS  PubMed  Google Scholar 

  21. Sala RG, Andrade FH, Cerono JC. Quantitative trait loci associated with grain moisture at harvest for line per se and testcross performance in maize: a meta-analysis. Euphytica. 2012;185(3):429–40.

    Article  Google Scholar 

  22. Liu J, Yu H, Liu Y, Deng S, Liu Q, Liu B, et al. Genetic dissection of grain water content and dehydration rate related to mechanical harvest in maize. BMC Plant Biol. 2020;20(1):118–33.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Yin S, Liu J, Yang T, Li P, Xu Y, Fang H, et al. Genetic analysis of the seed dehydration process in maize based on a logistic model. Crop J. 2020;8(02):182–93.

    Article  Google Scholar 

  24. Austin DF, Lee M, Veldboom LR, Hallauer AR. Genetic mapping in maize with hybrid progeny across testers and generations: grain yield and grain moisture. Crop Sci. 2000;40(1):30–8.

    Article  Google Scholar 

  25. Latrasse D, Germann S, Houba-Hérin N, Dubois E, Bui-Prodhomme D, Hourcade D, et al. Control of flowering and cell fate by LIF2, an RNA binding partner of the polycomb complex component LHP1. PLoS One. 2011;6(1):e16592.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Misevic D, Alexander DE. Twenty four cycles of phenotypic recurrent selection for percent oil in maize: 1. Per se and test-cross performance. Crop Sci. 1989;29(2):320–4.

    Article  CAS  Google Scholar 

  27. Jiao Y, Peluso P, Shi J, Liang T, Stitzer MC, Wang B, et al. Improved maize reference genome with single-molecule technologies. Nature. 2017;546(7659):524–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Mingjian H, Haiming Z, Bo Y, Shuang Y, Haihong L, He T, et al. ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels. New Phytol. 2021;232(6):2384–99.

    Article  CAS  Google Scholar 

  29. Dai L, Wu L, Dong Q, Zhang Z, Wu N, Song Y, et al. Genome-wide association study of field grain drying rate after physiological maturity based on a resequencing approach in elite maize germplasm. Euphytica. 2017;213(8):182–93.

    Article  CAS  Google Scholar 

  30. Zhou G, Hao D, Xue L, Chen G, Lu H, Zhang Z, et al. Genome-wide association study of kernel moisture content at harvest stage in maize. Breed Sci. 2018;68(5):622–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Jia T, Wang L, Li J, Ma J, Cao Y, Lübberstedt T, et al. Integrating a genome-wide association study with transcriptomic analysis to detect genes controlling grain drying rate in maize (Zea may, L.). Theor Appl Genet. 2020;133(2):623–34.

    Article  CAS  PubMed  Google Scholar 

  32. Zhang J, Guo J, Liu Y, Zhang D, Zhao Y, Zhu L, et al. Genome-wide association study identifies genetic factors for grain filling rate and grain drying rate in maize. Euphytica. 2016;212(2):201–12.

    Article  CAS  Google Scholar 

  33. Ruirui Y, Tao W, Wensen S, Siyu L, Zhibin L, Jianmei W, et al. E3 ubiquitin ligase ATL61 acts as a positive regulator in abscisic acid mediated drought response in Arabidopsis. Biochem Biophys Res Commun. 2020;528(2):292–8.

    Article  CAS  Google Scholar 

  34. Brugière N, Zhang W, Xu Q, Scolaro EJ, Lu C, Kahsay RY, et al. Overexpression of RING domain E3 ligase ZmXerico1 confers drought tolerance through regulation of ABA homeostasis. Plant Physiol. 2017;175(3):1350–69.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  35. Chen J, Zeng B, Zhang M, Xie S, Wang G, Hauck A, et al. Dynamic Transcriptome landscape of maize embryo and endosperm development. Plant Physiol. 2014;166(1):252–64.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  36. Yi F, Gu W, Chen J, Song N, Gao X, Zhang X, et al. High temporal-resolution Transcriptome landscape of early maize seed development. Plant Cell. 2019;31(5):974–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Gallie DR, Young TE. The ethylene biosynthetic and perception machinery is differentially expressed during endosperm and embryo development in maize. Mol Gen Genomics. 2004;271(3):267–81.

    Article  CAS  Google Scholar 

  38. Liu L, Duan L, Zhang J, Mi G, Zhang X, Zhang Z, et al. Arabidopsis LOS5 gene enhances chilling and salt stress tolerance in cucumber. J Integr Agric. 2013;12(5):825–34.

    Article  Google Scholar 

  39. Chen K, Li GJ, Bressan RA, Song CP, Zhu JK, Zhao Y. Abscisic acid dynamics, signaling, and functions in plants. J Integr Plant Biol. 2020;62(1):25–54.

    Article  CAS  PubMed  Google Scholar 

  40. Houben M, Van de Poel B. 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO): the enzyme that makes the plant hormone ethylene. Front Plant Sci. 2019;10:695.

    Article  PubMed  PubMed Central  Google Scholar 

  41. Nascimento FX, Rossi MJ, Glick BR. Ethylene and 1-Aminocyclopropane-1-carboxylate (ACC) in plant-bacterial interactions. Front Plant Sci. 2018;9:114.

    Article  PubMed  PubMed Central  Google Scholar 

  42. Li M, Yu G, Cao C, Liu P. Metabolism, signaling, and transport of jasmonates. Plant Commun. 2021;2(5):100231.

    Article  PubMed  PubMed Central  Google Scholar 

  43. Zhang D, Zhang M, Liang J. RGB1 regulates grain development and starch accumulation through its effect on OsYUC11-mediated Auxin biosynthesis in Rice endosperm cells. Front Plant Sci. 2021;12:585174–85.

    Article  PubMed  PubMed Central  Google Scholar 

  44. Wang R, Li M, Wu X, Wang J. The gene structure and expression level changes of the GH3 gene family in Brassica napus relative to its diploid ancestors. Genes. 2019;10(1):58.

    Article  PubMed Central  CAS  Google Scholar 

  45. Feng S, Yue R, Tao S, Yang Y, Zhang L, Xu M, et al. Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses. J Integr Plant Biol. 2015;57(9):783–95.

    Article  CAS  PubMed  Google Scholar 

  46. Casanova-Sáez R, Mateo-Bonmatí E, Ljung K. Auxin metabolism in plants. Cold Spring Harb Perspect Biol. 2021;13(3):a39867.

    Article  CAS  Google Scholar 

  47. Agliassa C, Narayana R, Bertea CM, Rodgers CT, Maffei ME. Reduction of the geomagnetic field delays Arabidopsis thaliana flowering time through downregulation of flowering-related genes. Bioelectromagnetics. 2018;39(5):361–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Huang B, Qian P, Gao N, Shen J, Hou S. Fackel interacts with gibberellic acid signaling and vernalization to mediate flowering in Arabidopsis. Planta. 2017;245(5):939–50.

    Article  CAS  PubMed  Google Scholar 

  49. Guillory A, Bonhomme S. Phytohormone biosynthesis and signaling pathways of mosses. Plant Mol Biol. 2021;107(4-5):245–77.

    Article  CAS  PubMed  Google Scholar 

  50. Chen L, Zhao J, Song J, Jameson PE. Cytokinin dehydrogenase: a genetic target for yield improvement in wheat. Plant Biotechnol J. 2019;18(3):614–30.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  51. Tomáš H, Lucia H, Neil ERJ. The hulks and the Deadpools of the Cytokinin universe: a dual strategy for Cytokinin production, translocation, and signal transduction. Biomolecules. 2021;11(2):209–46.

    Article  CAS  Google Scholar 

  52. DeWald DB, Sadka A, Mullet JE. Sucrose modulation of soybean Vsp gene expression is inhibited by Auxin. Plant Physiol. 1994;104(2):439–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. LeClere S, Schmelz EA, Chourey PS. Cell wall invertase-deficient miniature1 kernels have altered phytohormone levels. Phytochemistry. 2008;69(3):692–9.

    Article  CAS  PubMed  Google Scholar 

  54. Lefevere H, Bauters L, Gheysen G. Salicylic acid biosynthesis in plants. Front Plant Sci. 2020;11:338.

    Article  PubMed  PubMed Central  Google Scholar 

  55. Zhang Z, Hu Q, Liu Y, Cheng P, Cheng H, Liu W, et al. Strigolactone represses the synthesis of melatonin, thereby inducing floral transition in Arabidopsis thaliana in an FLC-dependent manner. J Pineal Res. 2019;67(2):e12582.

    Article  PubMed  CAS  Google Scholar 

  56. Yao R, Li J, Xie D. Recent advances in molecular basis for strigolactone action. Sci China Life Sci. 2018;61(3):277–84.

    Article  CAS  PubMed  Google Scholar 

  57. Li S, Zhao J, Dong S, Zhao M, Li C. Advances and prospects of maize cultivation in China. Sci Agric Sin. 2017;50(11):1941–59.

    Google Scholar 

  58. Dhurai SY, Reddy DM, Ravi S. Correlation and path analysis for yield and quality characters in Rice (Oryza sativa L.). Rice Genomics Genet. 2016;24:7.

  59. Calayugan MIC, Formantes AK, Amparado A, Descalsota-Empleo GI, Nha CT, Inabangan-Asilo MA, et al. Genetic analysis of agronomic traits and grain Iron and zinc concentrations in a doubled haploid population of Rice (Oryza sativa L.). Sci Rep. 2020;10(1):2283.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Mallimar M, Surendra P, Hundekar R, Jogi M, Lakkangoudar S. Correlation studies for micronutrients, yield and yield components in F3 population of rice (Oryza Sativa L.). Res Environ Life Sci. 2016;9(9):1140–2.

    Google Scholar 

  61. Arcade A, Labourdette A, Falque M, Mangin B, Chardon F, Charcosset A, et al. BioMercator: integrating genetic maps and QTL towards discovery of candidate genes. Bioinformatics. 2004;20(14):2324–6.

    Article  CAS  PubMed  Google Scholar 

  62. Darvasi A, Soller M. A simple method to calculate resolving power and confidence interval of QTL map location. Behav Genet. 1997;27(2):125–32.

    Article  CAS  PubMed  Google Scholar 

  63. Guo B, Sleper DA, Lu P, Shannon JG, Nguyen HT, Arelli PR. QTLs associated with resistance to soybean cyst nematode in soybean: Meta-analysis of QTL locations. Crop Sci. 2006;46(2):595–602.

    Article  Google Scholar 

  64. Chardon F, Virlon B, Moreau L, Falque M, Joets J, Decousset L, et al. Genetic architecture of flowering time in maize as inferred from quantitative trait loci meta-analysis and synteny conservation with the rice genome. Genetics. 2004;168(4):2169–85.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Xiao Y, Tong H, Yang X, Xu S, Pan Q, Qiao F, et al. Genome-wide dissection of the maize ear genetic architecture using multiple populations. New Phytol. 2016;210(3):1095–106.

    Article  CAS  PubMed  Google Scholar 

  66. Ginestet C. ggplot2: elegant graphics for data analysis. J R Stat Soc. 2011;174(1):245–6.

    Article  Google Scholar 

  67. Shi W, Shao H, Sha Y, Shi R, Shi D, Chen Y, et al. Grain dehydration rate is related to post-silking thermal time and ear characters in different maize hybrids. J Integr Agric. 2022;21(4):964–76.

    Article  CAS  Google Scholar 

  68. Liu X, Yu Q, Dou K, Guo X, Yao D, Hao R, et al. Effect of planting density on grouting and dehydration of spring maize in eastern Hebei Province. Guangdong Agric Sci. 2021;48(02):1–10.

    Google Scholar 

Download references

Acknowledgments

The authors thank Dongdong Li, PhD, China Agricultural University, for his excellent advice on the meta-analysis.

Funding

This work was supported by the National Natural Science Foundation of China (32001558 to F.Y.).

Author information

Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, Engineering Research Center of Plant Growth Regulator, Ministry of Education &College of Agronomy and Biotechnology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian, Beijing, 100193, China

    Wei Wang, Zhaobin Ren, Lu Li, Yiping Du, Yuyi Zhou, Mingcai Zhang, Zhaohu Li, Fei Yi & Liusheng Duan

  2. College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China

    Liusheng Duan

Authors
  1. Wei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhaobin Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yiping Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuyi Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mingcai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhaohu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fei Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Liusheng Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

L.D. and F.Y. designed and conceived the experiments. W.W., Z.R., L. L. and Y. D carried out the experiments, analyzed and interpreted the data. W.W. and F.Y. prepared the manuscript. Y.Z., M.Z. and Z.L. conceived the study and participated in its design. L.D. and F. Y revised the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Fei Yi.

Ethics declarations

Ethics approval and consent to participate

We declare that the experiments obey the ethical standards of the IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora.

Competing interests

The authors declare no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, W., Ren, Z., Li, L. et al. Meta-QTL analysis explores the key genes, especially hormone related genes, involved in the regulation of grain water content and grain dehydration rate in maize. BMC Plant Biol 22, 346 (2022). https://doi.org/10.1186/s12870-022-03738-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s12870-022-03738-y

Keywords

BMC Plant Biology

ISSN: 1471-2229

Contact us