Transporter NRT1.5/NPF7.3 suppresses primary root growth under low K+ stress by regulating the degradation of PIN-FORMED2
BMC Plant Biology volume 22, Article number: 330 (2022)
The availability of potassium is one of the main environmental factors for modifying the plasticity of root architecture. Many potassium channels and transporters are involved in regulating primary root growth in response to low potassium stress. NRT1.5/NPF7.3 transporter is a NO3−/H+ and K+/H+ cotransporter, and participates in NO3− and K+ translocation from the roots to the shoots. However, the underlying mechanism of NRT1.5-regulated primary root growth under low potassium stress is unclear.
We show that NRT1.5/NPF7.3 inhibited primary root growth under low potassium conditions by regulating the accumulation of PIN2 protein and auxin levels. Under low potassium conditions, the mutants nrt1.5 and lks2 exhibited longer primary roots, longer meristem regions and elongation zones of primary roots, and more cell activity in the meristem region compared to WT plants, revealing the involvement of NRT1.5 in LK (low potassium)-inhibition primary root growth. In addition, exogenous auxin (IAA), auxin analogue (NAA, 2.4-D) or auxin precursor (IBA) promoted the primary root growth of WT and the complementation line NRT1.5 COM plants. In addition, the application of NPA inhibited the primary root growth of the nrt1.5 and lks2 mutants. Auxin accumulation was higher in the root tip of nrt1.5 plants than in WT plants, indicating that NRT1.5 regulates root growth inhibition by regulating auxin distribution. Furthermore, PIN2 was degraded more quickly in nrt1.5 plants under LK stress.
Our findings reveal that NRT1.5 inhibits primary root growth by modulating the auxin level in the root tip via the degradation of PIN2.
As sessile organisms, plants have to properly modify their organ development to enhance their ability to capture resources. In particular, the plasticity of root architecture is a highly adaptive response that enables plants to counteract the spatial and temporal changes in the availability of water and mineral ions with different foraging strategies [1, 2]. Nutrient availability can exert a profound impact on root architecture. When grown under limited phosphorus availability, plants show inhibition of primary root elongation and increase the initiation of lateral roots [3,4,5]. In contrast to low phosphorus stress, low nitrogen promotes the growth of primary roots and lateral roots [3, 6].
Potassium (K+), the most abundant cation in living plant cells, plays an essential role in plant growth and development . However, the K+ concentration in soils is low and fluctuates [8, 9]. In addition, the availability of K+ in soils is spatially heterogeneous. Therefore, plants must modulate their root architecture to forge potassium. In Arabidopsis thaliana, K+ deficiency impairs primary root and lateral root development while inducing root hair elongation [10, 11]. Interestingly, diverse species of plants show different responses to K+ deficiency [12,13,14]. For example, low potassium stress reduces the lateral root elongation of Arabidopsis (Col-0) [12, 13], but increases the overall lateral root growth of barley seedlings .
The phytohormone auxin plays crucial roles in plant responses to K+ deficiency [15, 16]. The auxin level of roots is downregulated under LK conditions . The K+ transporters KUP2/6/8 regulate lateral root formation by modulating auxin signalling . In addition, the detection of ProDR5:GUS expression in roots showed that LK stress induces auxin redistribution in primary roots , indicating that auxin distribution is essential for root growth in response to LK stress. Moreover, KUP4 and KUP9, two members of the K+ transporter KUP/HAK family, function as auxin transporters [19,20,21]. The K+ transporter KUP4/TRH1 regulates root hair development and root gravitropism by modulating auxin transport [19, 22]. KUP9 mediates auxin efflux from the ER lumen to the cytoplasm, and regulates primary root growth in response to low potassium stress .
Auxin transport in roots through influx and efflux carriers (such as AUX1 and PINs) promotes auxin-dependent root growth in response to potassium deficiency. Auxin is mobilized via AUX1, AXR1, and AXR2 to root tips to inhibit primary root growth under LK conditions . The inhibition of primary root growth by K+ deficiency can be reversed in auxin-resistant mutants . Auxin transport towards the root tips is maintained by basally localized PIN1, PIN3 and PIN7 in the stele . PIN2 localizes at the epidermal and cortex cells of root tips and mediates the upward and downward flow of auxin from the root tip towards the elongation zone, creating an auxin gradient in roots together with other transporting proteins [25, 26]. The accumulation and subcellular localization of PIN proteins are essential for the formation of the auxin gradient, which is important for the root development response to different stresses [22, 27, 28]. Previous studies have indicated that potassium transporter proteins participate in regulating the accumulation and subcellular localization of PIN proteins. KUP4/TRH1 regulates the distribution of the auxin efflux carrier PIN1 [19, 22, 27]. In addition, the potassium channel AKT1 regulates K+-dependent root growth by modulating auxin transporter PIN1 degradation and auxin redistribution in the root . PIN2 is involved in root hair growth and primary root growth after LP or Peps (plant elicitor peptides) treatment [29, 30]. Whether PIN2 functions in primary root growth under potassium deficiency is still unclear.
NRT1.5/NPF7.3 acts as a NO3−/H+ and K+/H+ cotransporter, and participates in NO3− and K+ translocation from the roots to the shoots [31, 32]. NRT1.5 also functions as the transporter of IBA, the precursor of auxin indole-3-acetic acid (IAA), and regulates root gravitropism . NRT1.5/NPF7.3 belongs to the NRT1/PTR family, which contains as many as 53 members. Some members of this family have been shown to participate in regulating phytohormone-mediated plant growth and development [34,35,36]. NRT1.1 plays a key role in triggering the stimulation of LR elongation through auxin-signalling pathways . NPF3 can mediate GA transmembrane transportation, and participates in seed germination, hypocotyl, and primary root growth . NRT1.2 can act as an ABA transporter to regulate root growth .
NRT1.5 has been reported to transport IBA and regulate the synthesis of auxin . Here we demonstrated that NRT1.5 has a new function in regulating auxin distribution. Our results showed that NRT1.5 regulated the degradation of PIN2 and promoted upward auxin flow, resulting in a decrease in auxin accumulation in root tips, thereby suppressing root growth under LK conditions.
NRT1.5 participates in LK-mediated inhibition of primary root growth
The nrt1.5 mutant maintains primary root growth under low K+ stress. NRT1.5, one of the most important transporters in Arabidopsis, conducts K+ and NO3− translocation from the roots to the shoots [31, 32]. Our previous report showed that the nrt1.5 mutant displays a phenotype sensitive to low K+ stress, and exhibits chlorosis of the leaves on LK medium . However, it is interesting that the primary root of the nrt1.5 mutant continues to grow, but the wild-type root stops growing after transfer from MS to LK medium . This result suggested that NRT1.5 may play an important role in regulating primary root growth under low potassium stress. In this study, we explored the mechanism of NRT1.5 in regulating primary root growth under low potassium stress.
Both cell division in the meristem zone (MZ) and cell elongation in the elongation zone (EZ) contribute to root growth. To evaluate the role of NRT1.5 in LK-mediated primary root inhibition, we initially observed and measured the meristem zone and elongation zone of primary roots under LK stress. It has been reported that LKS2 is a point-mutational form of NRT1.5 . Therefore, the lks2 (point mutation) and nrt1.5 (T-DNA insertion) mutants were used in this study. We transferred 4-d-old seedlings to LK medium or MS medium for 3 days. In MS medium, there was no obvious difference in the length of MZ and EZ between mutants (nrt1.5 and lks2) and wild-type plants (Fig. 1). However, under LK stress, the meristem zone and elongation zone in the lks2 and nrt1.5 mutants were longer than those in the wild-type plants (Fig. 1). It is worth noting that the meristem of wild-type became dark after a period of growth in LK medium. The meristem is the source of all root cells [37, 38]. The short and dark meristem zones in wild-type plants suggest that meristem activity may be impaired in wild-type plants under LK conditions. To explore the cell activity in various plants under LK conditions, we finally examined the cell activity by trypan blue staining. The results showed that cells in the MZ of the wild-type and NRT1.5 COM plants were stained by trypan blue after 3 days of low potassium treatment, while the cells in lks2 and nrt1.5 plants were not stained (Fig. S1), indicating that cell death occurred in the MZ of wild-type plants after low potassium treatment, which was consistent with the dark root tip of wild-type (Fig. 1).
Then we measured the growth of primary root in various plants after LK treatment for 3 days. We transferred 4-d-old seedlings to LK medium and measured the primary root growth after 3-day treatment. We found that the primary root growth of wild-type plants was blocked in LK medium (Fig. 2). However, the primary roots of the lks2 and nrt1.5 mutants continued growing under LK stress (Fig. 2). The complementation line NRT1.5 COM was able to rescue the primary root growth of nrt1.5 plants to wild-type plant levels (Fig. 2). Taken together, these results suggest that NRT1.5 repress the growth of primary roots by regulating the cell activity, cell division, cell elongation in the root tip response to LK stress.
Auxin participates in NRT1.5-suppressed root growth
The biosynthesis and transport of the phytohormone auxin are critical in regulating primary root growth in response to LK. We speculated that NRT1.5 may regulate primary root growth through auxin signalling. To monitor the auxin distribution in roots after LK treatment, a biosensor ProDR5:GUS line  containing DIRECT REPEAT 5 (DR5)-driven GUS was crossed with the nrt1.5 mutant. As shown in Fig. 3, there was no obvious difference in the GUS signals between wild-type and the nrt1.5 mutant after transferred to MS or LK medium for 0.5 days and 1 day (Fig. 3). But the GUS signals in the wild-type root tips was significantly declined after transferred to LK medium for 2 days and 3 days, while these signals in the nrt1.5 root tips were stable (Fig. 3). The results indicated that the nrt1.5 mutant possessed a higher auxin concentration in the primary root tip than wild-type under LK stress, which was consistent with the root growth phenotype (Fig. 2). These data confirm that NRT1.5 regulates primary root growth through regulating the accumulation or distribution of auxin.
To verify whether the long-root phenotype of nrt1.5 mutants is dependent on auxin, we treated plants with endogenous auxin IAA (indole-3-acrtic acid), its synthesis precursor IBA (indole-3-butyric acid), and synthetic auxin analogue 2.4-D. We found that both IAA and 2.4-D dramatically inhibited root growth nrt1.5 mutant, but didn’t significantly inhibit root growth in Col (Fig. 4). Interestingly, the addition of 25 nM IBA significantly enhanced the wild-type root growth, and inhibited root growth in nrt1.5 seedlings. Higher doses of IBA at concentrations more than 50 nM dramatically inhibited nrt1.5 root growth, but did not significantly enhanced wild-type root growth (Fig. 4), suggesting that NRT1.5-regulated primary root growth is related to auxin concentration. Therefore, we blocked auxin biosynthesis with TAA1/TAR inhibitor Kyn (L-kynurenine), and found that Kyn also have an inhibitory effect on nrt1.5 mutant root growth, we also added the auxin NAA (1-naphthaleneacetic acid) into MS and LK medium. After addition of NAA into the LK medium, primary root growth was affected. On LK medium, exogenous NAA promoted the primary roots of the wild-type and the complementation line plants, but there was no effect on the primary roots of the nrt1.5 and lks2 plants (Fig. 5). Moreover, the auxin transport inhibitor NPA (N-1-naphtylphthalamic acid) inhibited the primary root growth of nrt1.5 and lks2 (Fig. 5), further supporting that our hypothesis that NRT1.5 regulates primary root growth by regulating auxin accumulation.
We assumed that auxin signalling is involved in NRT1.5-regulated primary root growth under LK conditions. The application of auxins increased the auxin concentration in both wild-type and nrt1.5 seedlings primary root. As auxins regulate root growth in a dose-dependent manner, the increase of auxin concentration promoted root growth in wild-type, but inhibited the root growth of nrt1.5 mutant, which is consistent with the expression of ProDR5:GUS. Furthermore, both auxin polar transport inhibitor (NPA) and auxin biosynthesis inhibitor (Kyn) inhibited the primary root growth of nrt1.5 plants, further confirming the hypothesis that NRT1.5-inhibited primary root growth is dependent on auxin.
NRT1.5 reduces PIN2 abundance under LK stress
The local auxin concentration in the root apex is highly determined by polar auxin transport. In Arabidopsis, PIN family proteins, especially PIN1, PIN2, PIN3, PIN4, and PIN7, are components of the auxin efflux machinery that precisely mediate polar auxin transport and function in roots [40,41,42,43]. Given that the auxin level in nrt1.5 root tips is higher than that in Col root tips, we reasoned that NRT1.5 might modify polar auxin transport in the root under LK stress. To test this idea, we examined the expression and protein abundance of PINs. First, we analyzed the mRNA levels of PIN2 and PIN3 in nrt1.5 and wild-type plants. The results showed that the PIN2 transcript in the wild-type roots was markedly induced when plants were transferred from MS to LK medium (Fig. S2). However, the PIN2 transcript in the nrt1.5 mutant was weakly induced after LK treatment (Fig. S2). RT-qPCR analysis revealed that the PIN3 transcript in wild-type and nrt1.5 plants was inhibited. In addition, the PIN3 transcript in nrt1.5 plants were higher than that in wild-type in both MS plants and LK media (Fig. S2). Second, we crossed ProPIN2:PIN2-GFP or ProPIN3:PIN3-GFP with nrt1.5 plants to obtain the ProPIN2:PIN2-GFP/nrt1.5 and ProPIN3:PIN3-GFP/nrt1.5 plants and examined the distribution and abundance of PIN2 and PIN3 under low potassium stress. We observed similar PIN2 fluorescence distribution patterns in wild-type and nrt1.5 roots before LK treatment (Fig. 6). We then examined the effect of low potassium stress on PIN2-GFP abundance in the nrt1.5 mutant and wild-type plants. The levels of PIN2-GFP fluorescent signals in wild-type roots were comparable within 6 h of LK treatment. After 9 h of LK treatment, the PIN2-GFP signals were slightly decreased in wild-type plants. However, PIN2 levels in nrt1.5 mutants obviously decreased within 3 h of LK treatment (Fig. 6). The PIN2-GFP signals in nrt1.5 plants maintained a very low level within 6 h, 9 h, and 12 h of LK treatment (Fig. 6). When compared with wild-type plants, nrt1.5 showed weaker PIN2-GFP fluorescent signals under LK stress. The plasma membrane (PM)-located PIN2 proteins cycle between the PM and endosomal compartments, which in turn determines the direction and activity of auxin transport . To explore whether NRT1.5 may alter the subcellular localization of PIN2, a more-detailed view of PIN2-GFP was observed after LK treatment for 3 h and 6 h. As shown in Fig. S3, PIN2-GFP abundance was reduced after LK treatment in nrt1.5 mutant, but PIN2-GFP-labeled endosomal compartments wasn’t detected. We also examined similar PIN3-GFP fluorescence distribution patterns in wild-type and nrt1.5 roots under MS and LK medium (Fig. S4). These results showed that the lack of NRT1.5 decreased PIN2 accumulation, implying that NRT1.5 plays a negative role in regulating the degradation of PIN2 under LK stress.
The availability of mineral nutrients in the soil is heterogeneous, which is one of the main factors limiting crop production. To capture more resources for high crop production, plant roots have to respond to nutritional status. The root system architectural response is one of the most important strategies for adapting to fluctuating nutrient availability in the soil [1, 2]. To date, we have a detailed understanding of the effects of nutrients, especially low nutrient stresses, on primary root growth [3, 4, 28]. In Arabidopsis, low Pi and low K+ stress results in a reduction in primary root length, while low N stress enhances primary root growth [3, 4, 28]. In this study, we found that low potassium inhibited primary root growth, which is consistent with a previous report .
NRT1.5 is an important transporter that translocates NO3− and K+ from the roots to the shoots, regulating tolerance to stresses (salt, drought and cadmium), and lateral root development [31, 32, 45, 46]. Our data indicate that NRT1.5 has a major role in regulating primary root growth under low potassium stress, leading to a decrease in primary root growth. Both mutants (nrt1.5 and lks2) showed strongly increased primary root growth under low potassium conditions (Fig. 2). Moreover, the length of the meristem zone and elongation zone were strongly increased in the nrt1.5 mutants (Fig. 1), which indicated that NRT1.5 regulated cell division and elongation under low potassium stress.
Auxin is a key regulator of root plastic growth, and its abundance decreases in the root tip under potassium deficiency . External application of NAA significantly alleviated LK-induced root growth inhibition (Fig. 5), indicating that the reduction in auxin levels in the root tip is the main reason for primary root growth inhibition. Moreover, primary root growth is affected by polar auxin transport under low potassium conditions. Previous studies revealed that potassium transporters are involved in auxin-regulated root growth by regulating auxin redistribution and the auxin response [18, 20, 27, 28]. TRH1/KUP4 may possess auxin transport activity, and regulates localization of the auxin efflux carrier PIN1, root hair development and root gravitropism [19, 20, 27]. KUP2/6/8 regulate the auxin response and lateral root formation . KUP9 functions as an auxin efflux carrier in QC cells and regulates root growth under low potassium stress . AKT1 was reported to be required for the regulation of K+-dependent root growth by modulating PIN1 degradation and auxin redistribution in the root . Here, we demonstrated that NRT1.5 may alleviate the degradation of PIN2 under LK, maintained upward auxin recycling, and decreased the auxin level in wild-type roots in LK medium. LK treatment induced the degradation of PIN2 in nrt1.5 roots. The degradation of PIN2 decreased the increase in auxin flow and maintained auxin levels for root growth, resulting in primary root growth under low potassium. These results indicated that NRT1.5 may regulate auxin redistribution in primary roots under LK stress. Moreover, NRT1.5 directly mediates indole-3-butyric acid (IBA), a precursor of IAA, in yeast . These data suggest that KUP4, KUP9, AKT1, and NRT1.5 coordinately control Arabidopsis root growth by regulating auxin homeostasis.
Local auxin distribution is achieved by the distinct localization of PIN proteins, which dynamically change in response to developmental cues, such as nutrient levels of the soil. PIN2, which localizes at the cortex and in epidermal cells, channels auxin flow from the root tip towards the elongation zone, creating the auxin gradient in roots together with other auxin transporting proteins [5, 47, 48]. Auxin efflux carriers PIN2 is important for modulation of root system architecture in response to different nutrient levels of the soil. In Arabidopsis thaliana, phosphorus deficiency promotes root hair number and length via suppressing vacuolar degradation of PIN2, thus enhancing root absorption capacity and efficiency . PIN2 also plays key role in regulating primary and lateral root growth in response to nitrate, as nitrate-treated pin2 mutants showed longer primary roots and less lateral roots number than wild-type plants . Furthermore, the phosphorylation status of PIN2, which regulates polar plasma membrane localization, is necessary for root system architecture modulation in response to nitrate. Here, we found that the degradation of PIN2 was more quickly in nrt1.5 mutant than wild-type under LK condition (Fig. 6), suggesting NRT1.5 may regulate the accumulation of PIN2 protein, accelerated the upward flow of auxin to the elongation zone, and blocked the auxin accumulation in the root tips.
In this study, we demonstrated the crucial roles of the transporter NRT1.5 in regulating primary root growth in response to LK stress. A mechanistic study indicated that NRT1.5 slowed the degradation of the auxin efflux carrier PIN2 and decreased auxin levels in the root tips.
Materials and methods
Plant materials and growth conditions
All the Arabidopsis thaliana seeds described in this manuscript were in Columbia (Col) ecotype. The seeds of Col, nrt1.5 , lks2 , ProDR5:GUS , ProPIN2:PIN2-GFP  and ProPIN3:PIN3-GFP  were obtained from Professor Weihua Wu’ Lab at China Agricultural University. The Col, nrt1.5 (SALK_043036) were originally from the Arabidopsis Biological Resource Center (ABRC, OhioState University, Columbus, OH, USA). The lks2 mutant was isolated from EMS-mutagenized Col, from Professor Weihua Wu’ Lab at China Agricultural University. The ProDR5:GUS is originally from Dr. Alonso at North Carolina State University, and ProPIN2:PIN2-GFP and ProPIN3:PIN3-GFP were originally from Dr. Scheres at Utrecht University. For generating ProDR5:GUS/nrt1.5, ProPIN2:PIN2-GFP/nrt1.5 and ProPIN3:PIN3-GFP/nrt1.5, the ProDR5:GUS, ProPIN2:PIN2-GFP and ProPIN3:PIN3-GFP reporter lines were genetically crossed with nrt1.5, respectively. The homozygous lines were identified by PCR and GFP fluorescence or GUS observation. Although newly hybrid plant materials are not deposited in publicly seed platform, they are available for other researchers from the corresponding author on reasonable request. And plants are used complying with relevant and national regulations.
Seeds were initially surface sterilized with 6% (v/v) of NaClO and 0.1% (v/v) Triton X-100 for 10 min and then washed with sterile water for five times. After stratification at 4 °C for 2 days in the dark, the sterilized seeds were sown on Murashige and Skoog (MS) medium with 0.8% agar and 3% (w/v) sucrose, and germinated at 22 °C under constant illumination at 80–90 μmol m− 2 s− 1 for 3 days. The LK medium was prepared by modification of MS medium described previously . KNO3 and KH2PO4 in MS medium were replaced by NH4NO3 and NH4H2PO4, respectively. So, the LK medium contained (in mM): 28.74 NH4NO3, 1.5 MgSO4·7H2O, 1.5 NH4H2PO4, 3 CaCl2·2H2O. The potassium almost came from agar in the LK medium.
For seed harvest and hybridization, Arabidopsis seedlings were grown in potting soil mixture (rich soil: vermiculite = 2:1, v/v) and kept in growth chambers at 22 °C with illumination at 120 μmol m− 2 s− 1 (16 h light/8 h dark period). The relative humidity was approximately 70% (±5%).
Root growth phenotype analyses
The seeds were germinated and grown vertically on MS medium for 4 days, and then transferred to MS or LK medium with or without the chemicals. In order to prepare MS or LK medium with different concentrations (10 nM 2.4-D, 50 nM 2.4-D, 25 nM IAA, 50 nM IAA, 25 nM IBA, 50 nM IBA, 0.1 μM Kyn, 1 μM Kyn, 30 nM NAA, 60 nM NAA) of chemicals, first prepare stock solutions (10 μM IAA, 100 μM 2,4-D, 50 μM IBA, 5 mM Kyn, and 100 μM NAA). Then corresponding volumes of stock solutions were added into medium until the medium was sterilized by autoclaving at 121 °C for 20 min and cooled to 60 °C.
The photographs were taken after transferring for 3 days. The length of primary root growth was measured with software Image J. Experiments were performed three times. Data were from 20 plants and presented as the mean ± SE. The student’s t test was used to analyze statistical significance. Asterisks in the figures denote significant differences: *P < 0.05, **P < 0.01.
For measuring the length of the meristem zone (MZ) and the elongation zone (EZ), the primary roots were imaged under a dissecting microscope (Nikon) equipped with a Canon DSLR camera. Three independent repetitious were performed. The student’s t test was used to analyze statistical significance. Asterisks in the figures denote significant differences: *P < 0.05, **P < 0.01.
After transferred to MS or LK medium for 3 days, the GUS reporter lines were incubated with GUS staining solution (1 mg mL− 1 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid, 0.1% Triton X-100, 10 mM EDTA, 2 mM potassium ferrocyanide, 2 mM potassium ferricyanide, and 50 mM sodium phosphate buffer, pH 7.2) for 3 hours. After staining, the seedlings were washed with 75% ethanol and then imaged under a dissecting microscope (Nikon) equipped with a Canon DSLR camera.
Trypan blue staining
For trypan blue staining, 4-day-old seedlings were transferred onto MS or LK medium for 3 days. The roots were stained with 0.4% Trypan blue for 5 min, rinsed 3 times, and photographed under a dissecting microscope (Nikon) equipped with a Canon DSLR camera.
Confocal laser scanning microscopy
After germination on MS medium for 4 days, the seedlings of GFP crossing lines were transferred to MS or LK medium for 3 h, 6 h, 9 h and 12 h. Images were captured on the confocal laser scanning microscope (Carl Zeiss LSM710 META). Scanner and detector settings were optimized to avoid saturation and to maximize resolution and kept unchanged throughout the experiment.
Availability of data and materials
All data supporting the findings of this study are available within the paper and within its supplementary data published online.
Nitrate Transporter 1.5
High-affinity K+ transporter/K+ uptake permease
Low K+ Sensitive 2
Polymerase Chain Reaction
Green Fluorescent Protein
Murashige and Skoog
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We thank Dr. Yi Wang for nrt1.5, lks2, ProDR5:GUS, ProPIN1:PIN1:GFP, ProPIN2:PIN2:GFP, ProPIN3:PIN3:GFP seeds.
This work was supported by the National Natural Science Foundation of China (31800198) and the Natural Science Foundation of Jiangsu Higher Education Institutions (18KJB180010) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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Trypan blue staining of wild-type and nrt1.5 roots. The 4-d-old seedlings were transferred onto LK and MS medium for 3 days. The roots were stained with 0.4% Trypan for 5 min and photographed under a dissecting microscope (Nikon) equipped with a Canon DSLR camera. Bars = 0.1 mm. Figure S2. The transcript of PIN2 and PIN3 in wild-type and nrt1.5 mutants under LK stress. The 4-d-old seedlings were transferred to LK and MS medium for 3 days. The roots were collected for analysis the transcript of PIN2 and PIN3. Figure S3. A more-detailed view of PIN2-GFP in wild-type and nrt1.5 mutant under LK stress. The ProPIN2:PIN2:GFP crossing lines were transferred to LK medium for 3 h and 6 h. The GFP fluorescence in plants roots was observed. Bars = 10 μm. Figure S4. Analysis the PIN3 in wild-type and nrt1.5 mutant under LK stress. The ProPIN3:PIN3:GFP crossing lines were transferred to LK medium for 3 d. The GFP fluorescence in plants roots was observed. Bar = 50 μm.
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Wang, Y., Wang, R., Zhao, S. et al. Transporter NRT1.5/NPF7.3 suppresses primary root growth under low K+ stress by regulating the degradation of PIN-FORMED2. BMC Plant Biol 22, 330 (2022). https://doi.org/10.1186/s12870-022-03730-6