Fig. 5

Overexpression of VaPYL4 enhances cold tolerance of transgenic Arabidopsis plants. A Freezing phenotypes of WT and OE plants. WT and OE plants were grown on 1/2 MS medium at 22℃ for 10 days before being subjected to freezing treatment. The plates were kept at -7℃ for 0.5 h and then incubated at 4℃ for 12 h. Representative photos were taken after recovery for 3 days under normal conditions. Scale bars correspond to 1 cm. B Survival rates of Arabidopsis plants after freezing treatment. C Electrolyte leakage of Arabidopsis plants after freezing treatment. Data are means ± SD. *P < 0.05; **P < 0.01. Similar results were observed in at least three independent experiments